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Stem Cells Derived from Pure Chorionic Trophoblast Layer and Cell Therapy Comprising Same

a technology of chorionic trophoblast layer and stem cells, which is applied in the direction of nerve system cells, skeletal/connective tissue cells, skeletal disorders, etc., can solve the problems of limited use as a cellular therapeutic agent, insufficient efficient commercialization, and insufficient research on stem cells derived from pure chorionic trophoblast layer which is a part of the tissues of the placenta. , to achieve the effect of excellent differentiation into cartilage cells,

Inactive Publication Date: 2016-11-10
SAMSUNG LIFE PUBLIC WELFARE FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention is about stem cells found in the pure chorionic trophoblast layer (which is without villi) of the placenta. These stem cells have better growth and differentiation characteristics compared to stem cells from the whole placenta. They can easily differentiate into cartilage cells, which makes them useful for treating cartilage damage and defects.

Problems solved by technology

For this disease treatment of the human, organ transplantation, gene therapy, and the like have been proposed before, but efficient commercialization is not sufficient due to immune rejection, supplied organs shortage, and lack of knowledge about vector development or disease genes.
Since the embryonic stem cells have ethical issue, there is a limitation to be used as a cellular therapeutic agent.
However, until now, researches on stem cells derived from a pure chorionic trophoblast layer which is a part of the tissues of the placenta are not yet conducted.

Method used

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  • Stem Cells Derived from Pure Chorionic Trophoblast Layer and Cell Therapy Comprising Same
  • Stem Cells Derived from Pure Chorionic Trophoblast Layer and Cell Therapy Comprising Same
  • Stem Cells Derived from Pure Chorionic Trophoblast Layer and Cell Therapy Comprising Same

Examples

Experimental program
Comparison scheme
Effect test

example 1

Preparing of Stem Cells Derived from a Pure Chorionic Trophoblast Layer which is a Part of Tissues of Placenta

[0065]The placenta was collected from the mother agreeing on donation in a normal cesarean delivery at the Samsung Seoul Hospital according to a guideline for clinical test ethics commission of the Samsung Seoul Hospital. The placenta tissue was put in a sterile container and then transferred. After an amniotic membrane was removed from the transferred placenta tissue, a chorionic trophoblast layer positioned between a chorionic membrane (CM) and decidua (DC) was isolated and a pure chorionic trophoblast layer (a chorionic trophoblast layer without villi, CT-V) of the chorionic trophoblast layer was carefully isolated by using sterilized forcep W and knife. A cross-sectional view of the placenta was illustrated in FIG. 1.

[0066]The isolated pure chorionic trophoblast layer tissue was washed several times by using PBS to remove blood and blood cells and then finely cut as much...

example 2

Subculture of Stem Cells Derived from Pure Chorionic Trophoblast Layer which is a Part of Tissues of Placenta

[0069]The stem cells derived from the pure chorionic trophoblast layer which was a part of the tissues of the placenta obtained in Example 1 were washed with PBS and cultured by replacing a DMEM medium including fetal bovine serum and antibiotics without a growth factor every 2 to 3 days. When the stem cells were grown 80% or more, the stem cells was treated with TryPLE to be isolated from the culture container, and the isolated stem cells were diluted in a ratio of ¼ and then cultured in another culture container to perform a subculture. While repetitively performing the subculture, the passage number which was not sub-cultured at all was measured, and cell forms before the subculture (P0) and after long-term subculture were observed with a microscope. Further, by using the stem cells derived from the whole placenta (Pla) obtained in Comparative Example 1, the subculture was...

example 3

Analysis of Colony Formation Capacity of Stem Cells Derived from Pure Chorionic Trophoblast Layer which is a Part of Tissues of Placenta

[0072]A colony formation capacity of the stem cells derived from the pure chorionic trophoblast layer as a part of the tissues of the placenta obtained in Example 1 was verified. More particularly, in the stem cells derived from the pure chorionic trophoblast layer obtained in Example 1, the first subculture was performed by the method of Example 2, and the stem cells were seeded by 5×103 in a dish of 100 mm at the time when the subculture was completed and then cultured in a DMEM medium including fetal bovine serum and antibiotics without a growth factor for 10 days. The number of colonies formed in the stem cells was counted by performing a Giemsa staining method in the cultured stem cells and an average value thereof was calculated. Further, by using the stem cells derived from the whole placenta obtained in Comparative Example 1, the colony form...

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Abstract

The present disclosure relates to stem cells derived from a pure chorionic trophoblast layer (chorionic trophoblast layer without villi, CT-V), which is a part of the tissues of the placenta, and cell therapy comprising same. Stem cells derived from a pure chorionic trophoblast layer according to the present invention exhibit uniform growth characteristic, and superb proliferation and differentiation characteristics compared to the conventional stem cells derived from the whole placenta, and particularly, exhibit excellent differentiation into cartilage cells, thus can be effectively used in cell therapy for treating cartilage damage, deficiency and such, and as a composition for tissue regeneration.

Description

TECHNICAL FIELD[0001]The present disclosure relates to stem cells derived from a pure chorionic trophoblast layer (chorionic trophoblast layer without villi, CT-V) which is a part of the tissues of the placenta and a cellular therapeutic agent including the same.BACKGROUND ART[0002]Recently, biotechnology has proposed the possibility of new solutions to the food, environmental, and health problems as a final goal of human welfare, and among them, a technique using stem cells is emerging as a new technique of incurable disease treatment. For this disease treatment of the human, organ transplantation, gene therapy, and the like have been proposed before, but efficient commercialization is not sufficient due to immune rejection, supplied organs shortage, and lack of knowledge about vector development or disease genes.[0003]As a result, an interest in the stem cells is increased, and totipotent stem cells having ability to generate all organs through proliferation and differentiation ar...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N5/073A61K35/50
CPCC12N5/0605C12N2506/025A61K35/50A61P19/00A61P19/02A61P19/04A61P19/08A61P25/00C12N5/0655C12N5/0668C12N2506/1392A61K35/12C12N5/0606C12N5/0619C12N5/0653C12N5/0654C12N5/066C12N2506/03
Inventor HA, CHUL WONKIM, JIN A.
Owner SAMSUNG LIFE PUBLIC WELFARE FOUND
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