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Compositions comprising a b subunit of shiga toxin and a means stimulating nkt cells

a technology of shiga toxin and nkt cells, which is applied in the field of antigen-related diseases, can solve the problems of unsafe or poorly immunogenic vaccines, laborious generation and ex vivo manipulation of dcs, and difficult production of vaccines, and achieve the effect of stimulating an immune respons

Inactive Publication Date: 2014-07-24
UNIV RENE DESCARTES PARIS V
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention is about a composition that can be used to treat antigen-related conditions in individuals. The composition includes a B subunit of Shiga toxin or a functional equivalent thereof which can bind to a specific receptor, and a ligand of CD1 that can stimulate natural killer T cells. The composition can be administered to individuals in a therapeutically effective amount to stimulate an immune response against the antigen and treat the antigen-related condition. The invention also includes a pharmaceutical kit and a vaccine comprising the composition.

Problems solved by technology

Live attenuated pathogens or whole inactivated organisms have been shown to activate both arms of the immune system in human but these vaccines are difficult to produce, potentially unsafe or poorly immunogenic.
Development of subunit vaccines to elicit a robust specific CD8+ T cell response represents therefore an ongoing competitive challenge.
However, generation and ex vivo manipulation of DCs are laborious.
However, when myeloid DCs derived from bone marrow were incubated with STxB, no maturation of these cells has been observed.
However, the Applicant observed and confirmed that all combinations of vectors and adjuvant are not equivalent for the induction of CD8+ T cells response.

Method used

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  • Compositions comprising a b subunit of shiga toxin and a means stimulating nkt cells
  • Compositions comprising a b subunit of shiga toxin and a means stimulating nkt cells
  • Compositions comprising a b subunit of shiga toxin and a means stimulating nkt cells

Examples

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examples

[0229]In the following description, all molecular biology experiments for which no detailed protocol is given are performed according to standard protocol.

[0230]The following examples are included to demonstrate preferred embodiments of the invention. It should be appreciated by those of skill in the art that the techniques disclosed in the examples which follow represent techniques discovered by the inventor to function well in the practice of the invention, and thus can be considered to constitute preferred modes for its practice. However, those of skill in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific embodiments which are disclosed and still obtain a like or similar result without departing from the spirit and scope of the invention.

[0231]I. Material and Methods.

[0232]Mice

[0233]Female C57BL / 6 (H-2b) mice were purchased from Charles River Laboratories (L'Arbresle, France). OT-I TCR transgenic (TG) mice specific for th...

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Abstract

The present invention relates to a composition comprising a) a B subunit of Shiga toxin or a functional equivalent thereof which is able to bind the Gb3 receptor, complexed with an antigen and b) at least one ligand of CDl capable of stimulating NK T cells; and to a pharmaceutical composition and a medicament comprising said composition.

Description

FIELD OF THE INVENTION[0001]The present invention relates to the field of antigen-related disease and especially to the field of therapeutic vaccines. In particular, the present invention aims to elicit an immune response in an individual in need thereof, using a B subunit of Shiga toxin or a functional equivalent thereof complexed to an antigen and a means stimulating NKT cells.BACKGROUND OF THE INVENTION[0002]Vaccine delivery system and adjuvants approved for human use (aluminium salts, MF59, virosomes . . . ) primarily stimulate humoral immune responses. However, preclinical studies strongly suggest that successful vaccines against pathogens (HIV, mycobacterium tuberculosis, malaria . . . ) but also cancer vaccines will likely require both humoral and cellular responses. Live attenuated pathogens or whole inactivated organisms have been shown to activate both arms of the immune system in human but these vaccines are difficult to produce, potentially unsafe or poorly immunogenic. ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K39/39
CPCA61K39/39A61K39/12A61K39/385A61K2039/5256A61K2039/55511A61K2039/55544A61K2039/55555A61K2039/6037A61P31/00A61P31/04A61P31/12A61P35/00A61P37/04C07K14/005C07K14/25C07K2319/00C12N7/00C12N2710/20022C12N2710/20034C12N2710/24143
Inventor TARTOUR, ERIC
Owner UNIV RENE DESCARTES PARIS V
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