A kind of method and application of cross pollination of celery

Abstract

The invention discloses a hybrid pollination method of celery and application of the method. The hybrid pollination method of celery comprises the following steps: firstly, carrying out transform breeding to obtain cytoplasmic male sterility (CMS for abbreviation) female parents and corresponding maintenance lines of the celery, selecting vernalized strong seed plants of CMS female parents and male parents to plant in pairs, arranging isolation net bags before bolting until flowering, carrying out artificial supplementary pollination in the flowering period, and ensuring that the CMS female parents in a hybridized combination only can obtain effective pollination of the male parents. The invention aims to ensure that the CMS female parent plants in the celery hybridized combination can obtain the effective pollination of the male parents; 100% pollen isolation between different combinations is implemented; the varietal complexity caused by mixture of pollen can be avoided; the breeding efficiency is improved.

Description

technical field

[0001] The invention belongs to the technical field of agricultural vegetable planting, and relates to a method and application of cross pollination of celery. Background technique

[0002] Celery is one of the important vegetable crops in my country. It belongs to the genus Celery of Umbelliferae. It is a biennial vegetable with entomophilous flowers. It is often cross-pollinated. The flowers are small and large, and the flower branches are soft and overlapping. The flowering period is long and scattered. It is very difficult to carry out strict cross-pollination artificially. Big. In the past, in order to ensure the purity of a variety, it was generally necessary to keep a natural isolation distance of more than 1,000 meters from other varieties during seed production. However, this approach is far from meeting the needs of modern commercial breeding work, because commercial breeding must follow the principle of high efficiency and precision, there are a lo...

Examples

Embodiment 1

[0036] The celery first-generation hybrid variety "Jinrui 1" with CMS traits was used to cross with the target transgenic material "Ventura", and F1 was backcrossed with "Ventura" for 4 consecutive generations to obtain the target CMS male sterile line female. This "C01-3A", "Ventura" became the corresponding maintainer line "C01-3B" of the CMS female parent "C01-3A" after selfing.

[0037] In spring, select the strong celery CMS female parent "C01-3A" and male parent "Qianfang" that have passed vernalization, remove the peripheral old leaves and the upper half of the petiole respectively, and prepare them with 1 million units of veterinary streptomycin Soak the roots with 1000 times the solution, and then plant the CMS female parent and male parent plants in pairs at a distance of 0.5 meters, and keep a distance of 0.8 meters between each pair of plants and adjacent plants. Immediately insert a field recording sign next to each pair of seed plants, step on the topsoil of the ...

Embodiment 2

[0042] The first-generation celery hybrid variety "Kuai Nen 60" with CMS traits was used to cross with the target transgenic material "Youventus", and F1 was backcrossed with "Youventus" for 4 consecutive generations to obtain the target CMS male sterile line The female parent "C10-80A" and "Youventus" became the corresponding maintainer line "C10-80B" of the CMS female parent "C10-80A" after selfing.

[0043] In spring, select the strong celery CMS female parent "C10-80A" and male parent "California Emperor" strains that have passed vernalization, remove the peripheral old leaves and the upper half of the petiole, and prepare them with 1 million units of veterinary streptomycin 1000 times the solution was soaked in the roots, and then the CMS female parent and male parent plants were planted in pairs at a distance of 0.5 meters, and a distance of 0.6 meters was maintained between each pair of plants and adjacent plants. Immediately insert a field recording sign next to each p...

technical field

[0001] The invention belongs to the technical field of agricultural vegetable planting, and relates to a method and application of cross pollination of celery. Background technique

[0002] Celery is one of the important vegetable crops in my country. It belongs to the genus Celery of Umbelliferae. It is a biennial vegetable with entomophilous flowers. It is often cross-pollinated. The flowers are small and large, and the flower branches are soft and overlapping. The flowering period is long and scattered. It is very difficult to carry out strict cross-pollination artificially. Big. In the past, in order to ensure the purity of a variety, it was generally necessary to keep a natural isolation distance of more than 1,000 meters from other varieties during seed production. However, this approach is far from meeting the needs of modern commercial breeding work, because commercial breeding must follow the principle of high efficiency and precision, there are a lo...

Examples

Embodiment 1

[0036] The celery first-generation hybrid variety "Jinrui 1" with CMS traits was used to cross with the target transgenic material "Ventura", and F1 was backcrossed with "Ventura" for 4 consecutive generations to obtain the target CMS male sterile line female. This "C01-3A", "Ventura" became the corresponding maintainer line "C01-3B" of the CMS female parent "C01-3A" after selfing.

[0037] In spring, select the strong celery CMS female parent "C01-3A" and male parent "Qianfang" that have passed vernalization, remove the peripheral old leaves and the upper half of the petiole respectively, and prepare them with 1 million units of veterinary streptomycin Soak the roots with 1000 times the solution, and then plant the CMS female parent and male parent plants in pairs at a distance of 0.5 meters, and keep a distance of 0.8 meters between each pair of plants and adjacent plants. Immediately insert a field recording sign next to each pair of seed plants, step on the topsoil of the ...

Embodiment 2

[0042] The first-generation celery hybrid variety "Kuai Nen 60" with CMS traits was used to cross with the target transgenic material "Youventus", and F1 was backcrossed with "Youventus" for 4 consecutive generations to obtain the target CMS male sterile line The female parent "C10-80A" and "Youventus" became the corresponding maintainer line "C10-80B" of the CMS female parent "C10-80A" after selfing.

[0043] In spring, select the strong celery CMS female parent "C10-80A" and male parent "California Emperor" strains that have passed vernalization, remove the peripheral old leaves and the upper half of the petiole, and prepare them with 1 million units of veterinary streptomycin 1000 times the solution was soaked in the roots, and then the CMS female parent and male parent plants were planted in pairs at a distance of 0.5 meters, and a distance of 0.6 meters was maintained between each pair of plants and adjacent plants. Immediately insert a field recording sign next to each p...

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