A kind of method for tissue culture and breeding of Dawu Lixinba

Abstract

A tissue culture breeding method for cymbaria dahurica comprises the following steps: (1) taking a cymbaria dahurica seed as an explant, and disinfecting the explant; (2) placing the disinfected explant in an MS minimal medium, and inducing the explant, so as to obtain a sterile test-tube plantlet; (3) placing the sterile test-tube plantlet in an MS breeding culture medium, and carrying out test-tube plantlet rapid breeding culture, so as to obtain cluster buds. According to the invention, the growth coefficient of the cymbaria dahurica cluster buds obtained by adopting the method can reach 15-25 times, and cymbaria dahurica high-grade seedlings with high survival rate can be provided in a short period, so that the problem about the large-scale seedling culture of cymbaria dahurica is effectively solved.

Description

technical field

[0001] The invention relates to a method for plant propagation, in particular to a method for tissue culture and propagation of Dauri simba. Background technique

[0002] Cymbaria dahurica L. is a plant of the genus Cymbaria dahurica in the family Scrophulariaceae, also known as big yellow flower and white Artemisia tea. The Mongolian medicine name is "Alatan-Agei". 1. It mostly grows in desert grasslands and mountain grasslands between 620-110 meters above sea level, and is mainly distributed in Inner Mongolia, Heilongjiang, Hebei and other places. The whole herb is used as medicine, which has the functions of drying "Xie Ri Wu Su", clearing away heat, dispelling rheumatism, diuresis, and hemostasis.

[0003] At present, the medicinal materials of Xinba on the market mainly come from wild resources. With the improvement of its medicinal value, the demand for medicinal materials in the market has increased, resulting in excessive mining of Dawuli Xinba, and ...

Examples

Embodiment 1

[0014] An example of the tissue culture rapid propagation method of Dawu Lixinba of the present invention, comprises the following steps:

[0015] (1) Selection and disinfection of explants: Take the plump fruit of Dauri simba, peel off the peel and take out the seeds as explants, soak the explants in 2v / v% detergent aqueous solution for 5min in turn, linear Rinse with tap water for 15-30min, add 2-3 drops of Tween-20 to 100ml of 0.1v / v% mercuric chloride for disinfection for 8-10min, rinse with sterile water for 3-5 times, and finally remove surface moisture with sterile filter paper to obtain bacterial explants;

[0016] (2) Induction of the first generation of explants to obtain sterile test-tube plantlets: inoculate the sterile explants obtained in step (1) into MS medium, at a culture temperature of 23-27°C, with a light intensity of 1500 lux and a light time of 8 Cultivate 45 days under the condition of -10 hours / day to obtain sterile test-tube plantlets, wherein in the...

Embodiment 2

[0019] Another example of the tissue culture rapid propagation method of Dawu Lixinba of the present invention comprises the following steps:

[0020] (1) Selection and disinfection of explants: Take the plump fruit of Dauri simba, peel off the peel and take out the seeds as explants, soak the explants in 2v / v% detergent aqueous solution for 5min in turn, linear Rinse with tap water for 15-30min, add 2-3 drops of Tween-20 to 100ml of 0.1v / v% mercuric chloride for disinfection for 8-10min, rinse with sterile water for 3-5 times, and finally remove surface moisture with sterile filter paper to obtain bacterial explants;

[0021] (2) Induction of the first generation of explants to obtain sterile test-tube plantlets: inoculate the sterile explants obtained in step (1) into MS medium, at a culture temperature of 23-27°C, with a light intensity of 1500 lux and a light time of 8 Cultivate 45 days under the condition of -10 hours / day to obtain sterile test-tube plantlets, wherein in...

Embodiment 3

[0024] Another example of the tissue culture rapid propagation method of Dawu Lixinba of the present invention comprises the following steps:

[0025] (1) Selection and disinfection of explants: Take the plump fruit of Dauri simba, peel off the peel and take out the seeds as explants, soak the explants in 2v / v% detergent aqueous solution for 5min in turn, linear Rinse with tap water for 15-30min, add 2-3 drops of Tween-20 to 100ml of 0.1v / v% mercuric chloride for disinfection for 8-10min, rinse with sterile water for 3-5 times, and finally remove surface moisture with sterile filter paper to obtain bacterial explants;

[0026] (2) Induction of the first generation of explants to obtain sterile test-tube plantlets: inoculate the sterile explants obtained in step (1) into MS medium, at a culture temperature of 23-27°C, with a light intensity of 1500 lux and a light time of 8 Cultivate 45 days under the condition of -10 hours / day to obtain sterile test-tube plantlets, wherein in...

technical field

[0001] The invention relates to a method for plant propagation, in particular to a method for tissue culture and propagation of Dauri simba. Background technique

[0002] Cymbaria dahurica L. is a plant of the genus Cymbaria dahurica in the family Scrophulariaceae, also known as big yellow flower and white Artemisia tea. The Mongolian medicine name is "Alatan-Agei". 1. It mostly grows in desert grasslands and mountain grasslands between 620-110 meters above sea level, and is mainly distributed in Inner Mongolia, Heilongjiang, Hebei and other places. The whole herb is used as medicine, which has the functions of drying "Xie Ri Wu Su", clearing away heat, dispelling rheumatism, diuresis, and hemostasis.

[0003] At present, the medicinal materials of Xinba on the market mainly come from wild resources. With the improvement of its medicinal value, the demand for medicinal materials in the market has increased, resulting in excessive mining of Dawuli Xinba, and ...

Examples

Embodiment 1

[0014] An example of the tissue culture rapid propagation method of Dawu Lixinba of the present invention, comprises the following steps:

[0015] (1) Selection and disinfection of explants: Take the plump fruit of Dauri simba, peel off the peel and take out the seeds as explants, soak the explants in 2v / v% detergent aqueous solution for 5min in turn, linear Rinse with tap water for 15-30min, add 2-3 drops of Tween-20 to 100ml of 0.1v / v% mercuric chloride for disinfection for 8-10min, rinse with sterile water for 3-5 times, and finally remove surface moisture with sterile filter paper to obtain bacterial explants;

[0016] (2) Induction of the first generation of explants to obtain sterile test-tube plantlets: inoculate the sterile explants obtained in step (1) into MS medium, at a culture temperature of 23-27°C, with a light intensity of 1500 lux and a light time of 8 Cultivate 45 days under the condition of -10 hours / day to obtain sterile test-tube plantlets, wherein in the...

Embodiment 2

[0019] Another example of the tissue culture rapid propagation method of Dawu Lixinba of the present invention comprises the following steps:

[0020] (1) Selection and disinfection of explants: Take the plump fruit of Dauri simba, peel off the peel and take out the seeds as explants, soak the explants in 2v / v% detergent aqueous solution for 5min in turn, linear Rinse with tap water for 15-30min, add 2-3 drops of Tween-20 to 100ml of 0.1v / v% mercuric chloride for disinfection for 8-10min, rinse with sterile water for 3-5 times, and finally remove surface moisture with sterile filter paper to obtain bacterial explants;

[0021] (2) Induction of the first generation of explants to obtain sterile test-tube plantlets: inoculate the sterile explants obtained in step (1) into MS medium, at a culture temperature of 23-27°C, with a light intensity of 1500 lux and a light time of 8 Cultivate 45 days under the condition of -10 hours / day to obtain sterile test-tube plantlets, wherein in...

Embodiment 3

[0024] Another example of the tissue culture rapid propagation method of Dawu Lixinba of the present invention comprises the following steps:

[0025] (1) Selection and disinfection of explants: Take the plump fruit of Dauri simba, peel off the peel and take out the seeds as explants, soak the explants in 2v / v% detergent aqueous solution for 5min in turn, linear Rinse with tap water for 15-30min, add 2-3 drops of Tween-20 to 100ml of 0.1v / v% mercuric chloride for disinfection for 8-10min, rinse with sterile water for 3-5 times, and finally remove surface moisture with sterile filter paper to obtain bacterial explants;

[0026] (2) Induction of the first generation of explants to obtain sterile test-tube plantlets: inoculate the sterile explants obtained in step (1) into MS medium, at a culture temperature of 23-27°C, with a light intensity of 1500 lux and a light time of 8 Cultivate 45 days under the condition of -10 hours / day to obtain sterile test-tube plantlets, wherein in...