Tissue Culture and Rapid Propagation Method of Orchid chinensis

Abstract

The invention discloses a method for tissue culture rapid propagation of orchid orchid orchids, comprising the following steps: step 1, taking newly germinated shoots of orchid orchid orchids as explants, placing them in the first culture medium for cultivation, and obtaining Sterile test-tube plantlets; step 2, placing the sterile test-tube plantlets obtained in step one in the second culture medium to obtain test-tube plantlets with clustered buds; step 3, placing the test-tube plantlets with clustered shoots obtained in step 2 in the third medium Cultivate to get strong seedlings; step 4, place the strong seedlings obtained in step 3 in the fourth culture medium to obtain rooted test-tube plantlets. The invention can rapidly and effectively improve the propagation speed and quality of the orchid orchid orchid seedlings, and realize industrial seedling cultivation of the high-quality orchid orchid orchid orchid seedlings, so as to meet the needs of production.

Description

Technical field

[0001] The invention belongs to the field of tissue culture and plant propagation, and in particular relates to a method for tissue culture and rapid propagation of C. vulgaris. Background technique

[0002] Bulbophyllum delitescens Hance (Bulbophyllum delitescens Hance) is a plant of the Orchidaceae Glycine max, with thick rhizomes, creeping roots, internodes covered by membranous sheaths or fibers remaining after the sheath rots, one at every 3-11cm interval Fake bulb. Scape originates from rhizome nodes with pseudobulbs, umbels with 2-4 flower petals sickle-lanceolate, apex is truncated and concave notch with 1 short awn in the center, with 3 veins , The whole edge. The lips are fleshy, tongue-like, and curved outwards. The flowers are unique and have a long flowering period, which is of great ornamental value. Seed reproduction is difficult to germinate due to incomplete embryo development. Coupled with the pressure of artificial excavation, the deteriora...

Examples

example 1

[0030] A method of tissue culture and rapid propagation of straight lip curly orchids includes the following steps:

[0031] Step 1. Take the newly germinated buds of C. serrata as explants and place them in the first culture medium to obtain sterile test tube plantlets; wherein, the first culture medium includes: Huabao No. 1 medium , 0.3mg / L methionazine, 1.5mg / L naphthaleneacetic acid, 1.5mg / L polyvinylpyrrolidone, 25g / L sucrose and 4.5g / L agar;

[0032] Step 2. Place the sterile test-tube plantlet obtained in step 1 in a second medium for culturing to obtain a clumping bud of the test-tube plantlet; wherein, the second medium includes: Huabao No. 1 medium, 0.5 mg / L A Thiadazine, 1.0mg / L naphthaleneacetic acid, 0.2mg / L kinetin, 1.5mg / L polyvinylpyrrolidone, 25g / L sucrose and 4.5g / L agar;

[0033] Step 3. Place the test-tube clumping buds obtained in step 2 in the third medium to obtain robust seedlings; wherein, the third medium includes: MS, 0.1 mg / L naphthalene acetic acid, 1.2...

example 2

[0047] A method of tissue culture and rapid propagation of straight lip curly orchids includes the following steps:

[0048] Step 1. Take the newly germinated buds of C. serrata as explants and place them in the first culture medium to obtain sterile test tube plantlets; wherein, the first culture medium includes: Huabao No. 1 medium , 0.7mg / L methionazine, 2.5mg / L naphthaleneacetic acid, 2.5mg / L polyvinylpyrrolidone, 35g / L sucrose and 5.5g / L agar;

[0049] Step 2. Place the sterile test-tube plantlet obtained in step 1 in a second medium for cultivation, to obtain a clumping bud of the test-tube plantlet; wherein, the second medium includes: Huabao No. 1 medium, 1.5 mg / L A Thiadazine, 2.5mg / L naphthaleneacetic acid, 0.5mg / L kinetin, 2.5mg / L polyvinylpyrrolidone, 35g / L sucrose and 5.5g / L agar;

[0050] Step 3. Place the test-tube clumping buds obtained in step 2 into the third medium to obtain robust seedlings; wherein, the third medium includes: MS, 0.3 mg / L naphthalene acetic acid...

example 3

[0064] A method of tissue culture and rapid propagation of straight lip curly orchids includes the following steps:

[0065] Step 1. Take the newly germinated buds of C. serrata as explants and place them in the first culture medium to obtain sterile test tube plantlets; wherein, the first culture medium includes: Huabao No. 1 medium , 0.5mg / L methionazine, 2mg / L naphthaleneacetic acid, 2mg / L polyvinylpyrrolidone, 30g / L sucrose and 5g / L agar;

[0066] Step 2. Place the sterile test tube plantlet obtained in step 1 in a second medium for cultivation to obtain a cluster of test tube plantlets; wherein the second medium includes: Huabao No. 1 medium, 0.5-1.5 mg / L Thioridazine, 1.0-2.5mg / L naphthaleneacetic acid, 0.2-0.5mg / L kinetin, 1.5-2.5mg / L polyvinylpyrrolidone, 25-35g / L sucrose and 4.5-5.5g / L Agar

[0067] Step 3. Place the test-tube clumping buds obtained in step 2 in the third medium to obtain robust seedlings; wherein, the third medium includes: MS, 0.2 mg / L naphthalene acetic...

Technical field

[0001] The invention belongs to the field of tissue culture and plant propagation, and in particular relates to a method for tissue culture and rapid propagation of C. vulgaris. Background technique

[0002] Bulbophyllum delitescens Hance (Bulbophyllum delitescens Hance) is a plant of the Orchidaceae Glycine max, with thick rhizomes, creeping roots, internodes covered by membranous sheaths or fibers remaining after the sheath rots, one at every 3-11cm interval Fake bulb. Scape originates from rhizome nodes with pseudobulbs, umbels with 2-4 flower petals sickle-lanceolate, apex is truncated and concave notch with 1 short awn in the center, with 3 veins , The whole edge. The lips are fleshy, tongue-like, and curved outwards. The flowers are unique and have a long flowering period, which is of great ornamental value. Seed reproduction is difficult to germinate due to incomplete embryo development. Coupled with the pressure of artificial excavation, the deteriora...

Examples

example 1

[0030] A method of tissue culture and rapid propagation of straight lip curly orchids includes the following steps:

[0031] Step 1. Take the newly germinated buds of C. serrata as explants and place them in the first culture medium to obtain sterile test tube plantlets; wherein, the first culture medium includes: Huabao No. 1 medium , 0.3mg / L methionazine, 1.5mg / L naphthaleneacetic acid, 1.5mg / L polyvinylpyrrolidone, 25g / L sucrose and 4.5g / L agar;

[0032] Step 2. Place the sterile test-tube plantlet obtained in step 1 in a second medium for culturing to obtain a clumping bud of the test-tube plantlet; wherein, the second medium includes: Huabao No. 1 medium, 0.5 mg / L A Thiadazine, 1.0mg / L naphthaleneacetic acid, 0.2mg / L kinetin, 1.5mg / L polyvinylpyrrolidone, 25g / L sucrose and 4.5g / L agar;

[0033] Step 3. Place the test-tube clumping buds obtained in step 2 in the third medium to obtain robust seedlings; wherein, the third medium includes: MS, 0.1 mg / L naphthalene acetic acid, 1.2...

example 2

[0047] A method of tissue culture and rapid propagation of straight lip curly orchids includes the following steps:

[0048] Step 1. Take the newly germinated buds of C. serrata as explants and place them in the first culture medium to obtain sterile test tube plantlets; wherein, the first culture medium includes: Huabao No. 1 medium , 0.7mg / L methionazine, 2.5mg / L naphthaleneacetic acid, 2.5mg / L polyvinylpyrrolidone, 35g / L sucrose and 5.5g / L agar;

[0049] Step 2. Place the sterile test-tube plantlet obtained in step 1 in a second medium for cultivation, to obtain a clumping bud of the test-tube plantlet; wherein, the second medium includes: Huabao No. 1 medium, 1.5 mg / L A Thiadazine, 2.5mg / L naphthaleneacetic acid, 0.5mg / L kinetin, 2.5mg / L polyvinylpyrrolidone, 35g / L sucrose and 5.5g / L agar;

[0050] Step 3. Place the test-tube clumping buds obtained in step 2 into the third medium to obtain robust seedlings; wherein, the third medium includes: MS, 0.3 mg / L naphthalene acetic acid...

example 3

[0064] A method of tissue culture and rapid propagation of straight lip curly orchids includes the following steps:

[0065] Step 1. Take the newly germinated buds of C. serrata as explants and place them in the first culture medium to obtain sterile test tube plantlets; wherein, the first culture medium includes: Huabao No. 1 medium , 0.5mg / L methionazine, 2mg / L naphthaleneacetic acid, 2mg / L polyvinylpyrrolidone, 30g / L sucrose and 5g / L agar;

[0066] Step 2. Place the sterile test tube plantlet obtained in step 1 in a second medium for cultivation to obtain a cluster of test tube plantlets; wherein the second medium includes: Huabao No. 1 medium, 0.5-1.5 mg / L Thioridazine, 1.0-2.5mg / L naphthaleneacetic acid, 0.2-0.5mg / L kinetin, 1.5-2.5mg / L polyvinylpyrrolidone, 25-35g / L sucrose and 4.5-5.5g / L Agar

[0067] Step 3. Place the test-tube clumping buds obtained in step 2 in the third medium to obtain robust seedlings; wherein, the third medium includes: MS, 0.2 mg / L naphthalene acetic...