Paracoccus sp. and culture application thereof

Abstract

The invention relates to paracoccus sp. and a culture application thereof. The strain is paracoccus sp. FSTB-2, collected at 'China General Microbiological Culture Collection Center' on June 1, 2015, and the preservation number is CGMCC No. 10938. The strain can be applied to efficiently removing COD (chemical oxygen demand) in salty wastewater with salt content of 1.0wt%-8.0wt%, and can grow at the high temperature of 40 DEG C. The invention further provides COD removing salt-tolerant bacteria which comprise the paracoccus sp. FSTB-2. The COD in the high-salt wastewater can be rapidly degraded by the paracoccus sp., the paracoccus sp. is high in salt resistance and good in treatment effect, combining strains are omitted, and the production cost of the salt-tolerant bacteria is greatly reduced.

Description

technical field

[0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a salt-tolerant deCOD paracoccus and its cultivation application. The bacterial strain is especially suitable for efficient removal of COD in high-salt wastewater. Background technique

[0002] Highly saline wastewater with a salt content higher than 1% from petroleum, chemical, pharmaceutical, and fertilizer industries generally contains high COD, and the continuous discharge of pollutants in wastewater into the environment will cause serious pollution problems. The shortage of fresh water resources is becoming more and more serious, and the treatment and recycling of waste water have been paid more attention by people.

[0003] Physical or chemical treatment of COD in high-saline wastewater is costly and may cause secondary pollution. Traditional biological methods have great advantages in treating low-salinity wastewater, but in high-salt, high-acid-bas...

Examples

Embodiment 2

[0028] Embodiment 2 screening and separation and purification

[0029] The activated sludge obtained from a petrochemical enterprise in Yueyang City, Hunan Province for the treatment of epichlorohydrin production wastewater was domesticated and cultivated for 15 days by gradually increasing the salt concentration in the culture medium.

[0030] The above-mentioned domesticated activated sludge was taken, and the method of dilution coating was used for preliminary separation in the enrichment medium, thereby obtaining 15 colonies with good growth status and different characters. The main components of the enrichment medium used are: 1000mg / L COD, 1000mg / L calcium ion concentration, 10000mg / L total salt, and 2.0% agar content in the solid medium used.

[0031] Then, the obtained colony was transferred by streaking on a plate, and transferred to a screening medium by streaking for further separation and screening. The main components of the screening medium used are: 1000mg / L CO...

Embodiment 3

[0033] The investigation of embodiment 3 paracoccus FSTB-2 salt tolerance

[0034] The cultivation method of Paracoccus FSTB-2 of the present invention comprises: bacterial strain activation, liquid seed solution cultivation, aeration cultivation, concrete process is as follows:

[0035] (1) Strain activation: Paracoccus ( Paracoccus sp. ) FSTB-2 was inoculated into a plate containing FSTB solid medium, cultured at 35°C for 48 hours, and then stored in a refrigerator at 4°C until use. The FSTB solid medium is: FeSO 4 •7H 2 O 25mg / L, NH 4 NO 3 286mg / L, KCl 929mg / L, CaCl 2 2769mg / L, NaCl 21008mg / L, beef extract 5g / L, peptone 10g / L, agar 20g / L, pH 7.8.

[0036] (2) Liquid seed liquid culture: Prepare FSTB liquid medium, divide it into Erlenmeyer flasks, sterilize and cool to room temperature, use an inoculation loop to pick the activated strains on the plate and inoculate them into Erlenmeyer flasks under a sterile environment. Incubate at 35°C for 48 hours. The FSTB ...

technical field

[0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a salt-tolerant deCOD paracoccus and its cultivation application. The bacterial strain is especially suitable for efficient removal of COD in high-salt wastewater. Background technique

[0002] Highly saline wastewater with a salt content higher than 1% from petroleum, chemical, pharmaceutical, and fertilizer industries generally contains high COD, and the continuous discharge of pollutants in wastewater into the environment will cause serious pollution problems. The shortage of fresh water resources is becoming more and more serious, and the treatment and recycling of waste water have been paid more attention by people.

[0003] Physical or chemical treatment of COD in high-saline wastewater is costly and may cause secondary pollution. Traditional biological methods have great advantages in treating low-salinity wastewater, but in high-salt, high-acid-bas...

Examples

Embodiment 2

[0028] Embodiment 2 screening and separation and purification

[0029] The activated sludge obtained from a petrochemical enterprise in Yueyang City, Hunan Province for the treatment of epichlorohydrin production wastewater was domesticated and cultivated for 15 days by gradually increasing the salt concentration in the culture medium.

[0030] The above-mentioned domesticated activated sludge was taken, and the method of dilution coating was used for preliminary separation in the enrichment medium, thereby obtaining 15 colonies with good growth status and different characters. The main components of the enrichment medium used are: 1000mg / L COD, 1000mg / L calcium ion concentration, 10000mg / L total salt, and 2.0% agar content in the solid medium used.

[0031] Then, the obtained colony was transferred by streaking on a plate, and transferred to a screening medium by streaking for further separation and screening. The main components of the screening medium used are: 1000mg / L CO...

Embodiment 3

[0033] The investigation of embodiment 3 paracoccus FSTB-2 salt tolerance

[0034] The cultivation method of Paracoccus FSTB-2 of the present invention comprises: bacterial strain activation, liquid seed solution cultivation, aeration cultivation, concrete process is as follows:

[0035] (1) Strain activation: Paracoccus ( Paracoccus sp. ) FSTB-2 was inoculated into a plate containing FSTB solid medium, cultured at 35°C for 48 hours, and then stored in a refrigerator at 4°C until use. The FSTB solid medium is: FeSO 4 •7H 2 O 25mg / L, NH 4 NO 3 286mg / L, KCl 929mg / L, CaCl 2 2769mg / L, NaCl 21008mg / L, beef extract 5g / L, peptone 10g / L, agar 20g / L, pH 7.8.

[0036] (2) Liquid seed liquid culture: Prepare FSTB liquid medium, divide it into Erlenmeyer flasks, sterilize and cool to room temperature, use an inoculation loop to pick the activated strains on the plate and inoculate them into Erlenmeyer flasks under a sterile environment. Incubate at 35°C for 48 hours. The FSTB ...

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