A cell culture dish that can apply a three-dimensional magnetic field

Abstract

A cell culture dish capable of applying a three-dimensional magnetic field comprises a culture dish, a culture dish cover is arranged on the top of the culture dish, the culture dish is fixedly arranged on a vertical magnetic field generating sheet, the vertical magnetic field generating sheet is used to generate a vertical magnetic field, a sheet support is arranged below the vertical magnetic field generating sheet, the vertical magnetic field generating sheet and the sheet support are movably connected, the sheet support is arranged on a base, magnet slots are arranged on the bases on both sides of the sheet support, electromagnets are arranged in the magnet slots, and the electromagnets are used to generate a horizontal magnetic field. The above structure can apply magnetic fields at multiple angles to the culture dish, so as to better study the influence of the magnetic field on cells.

Description

Technical Field

[0001] The invention relates to the field of cell culture devices, in particular to a cell culture dish capable of applying a three-dimensional magnetic field. Background Art

[0002] Cell culture refers to a method of isolating and culturing body cells or establishing cell lines under in vitro conditions to simulate the normal physiological environment and nutritional conditions in the body, and allowing cells to grow and proliferate for a long time in a culture container. Cell culture is carried out under artificial conditions, and has the advantages of being easy to control and observe. It has important application value in the fields of molecular genetics, molecular biology, modern medicine, biochemistry, etc. For example, fetal cells shed into amniotic fluid are obtained through amniocentesis, and chromosome analysis is performed after cell culture to diagnose whether the fetus has a genetic disease, providing technical support for eugenics. Recent studies have found that external field stimulation (such as electric field, gravity field, light field, magnetic field, etc.) may cause stress responses in cultured cells, thereby affecting their proliferation, differentiation, morphology, structure, function, and even the structure of their internal organelles. In-depth research on the effects of external fields on cells has important practical significance in the study of material metabolism, chromosome variation, expression and regulation of genetic material, etc. Research on the effects of external fields on cells not only poses new challenges to cell culture technology, but also puts forward new requirements for cell culture devices.

[0003] Magnetic field, as a special form of objective existence of matter, diffuses in the vast physical space. Studies have shown that specific magnetic fields may produce significant cell biological effects. For example, after several days of weak magnetic field irradiation, the cell division rate of Ochra tatarica will be significantly accelerated; steady-state magnetic field can reduce the content of cAMP in the cyclic adenosine monophosphate system of rat pineal gland; low-frequency magnetic field or static steady magnetic field has a significant effect on the proliferation of leukemia cell line (Jurkat Clone E6-1). Although some similar studies have been carried out, due to the large differences between cell types and the strong adjustability of magnetic field parameters, people still have a huge space for exploration in understanding the cell biological effects of magnetic field. At present, in such experimental studies, cell culture and magnetic field generation often use separate devices, which is not conducive to experimental operation and experimental control.

[0004] In summary, providing a cell culture dish device that can apply a three-dimensional magnetic field will greatly facilitate the study of the cell biological effects of magnetic fields and has important application value. At present, no similar device has been found in China. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a cell culture dish capable of applying a three-dimensional magnetic field, and to apply magnetic fields at multiple angles to the culture dish, so as to better study the influence of the magnetic field on cells.

[0006] In order to solve the above technical problems, the technical solution adopted by the present invention is: a cell culture dish that can apply a three-dimensional magnetic field, including a culture dish, a culture dish cover is provided on the top of the culture dish, the culture dish is fixedly set on a vertical magnetic field generating piece, the vertical magnetic field generating piece is used to generate a magnetic field in the vertical direction, a plate holding platform is provided below the vertical magnetic field generating piece, the vertical magnetic field generating piece and the plate holding platform are movably connected, the plate holding platform is set on a base, magnet grooves are provided on the base on both sides of the plate holding platform, electromagnets are provided in the magnet grooves, and the electromagnets are used to generate a magnetic field in the horizontal direction.

[0007] In a preferred solution, a fastening rod is provided on the bottom surface of the vertical magnetic field generating piece, a fastening rod fixing hole is provided at the center of the piece support table, and the fastening rod is inserted into the fastening rod fixing hole.

[0008] In a preferred solution, the fastening rod is provided with a threaded section, the threaded section of the fastening rod is provided with a nut, the nut is arranged on the fastening rod below the bottom surface of the wafer support platform, and an anti-slip handle is provided on the outer wall of the nut.

[0009] In a preferred embodiment, a marking line is provided on the side wall of the vertical magnetic field generating sheet, and an angular orientation marking line is provided on the side wall of the sheet support platform.

[0010] In a preferred solution, the base is divided into an upper base layer and a lower base layer, the upper base layer and the lower base layer are two cylindrical structures with different cross-sectional diameters, and the cross-sectional diameter of the upper base layer is smaller than that of the lower base layer, and the wafer support table is fixedly arranged on the upper base layer;

[0011] An inner ring fence and an outer ring fence are arranged on the top surface of the lower layer of the base, and the inner ring fence and the outer ring fence are fixedly arranged on the top surface of the lower layer of the base through fence support rods, and vertical support plates are arranged on the inner ring fence and the outer ring fence, and arc-shaped bayonet matching with the inner ring fence and the outer ring fence is arranged at the bottom of the support plate, and the bottom of the support plate is fixed to the inner ring fence and the outer ring fence by bite-engaging the arc-shaped bayonet;

[0012] The magnet slot is arranged on the top of the supporting plate.

[0013] In the preferred embodiment, the magnet slot is a "U"-shaped slot structure, the electromagnet is fixedly arranged in the magnet slot, a groove is arranged on the inner bottom surface of the magnet slot, an electromagnet wire is arranged in the groove, and a scale line is arranged on the lower edge of the outer wall of the magnet slot.

[0014] In a preferred embodiment, a bolt slot is provided on the side wall of the magnet slot, a horizontal fastening bolt is provided in the bolt slot, and the fastening end of the fastening bolt passes through the bolt slot and extends into the magnet slot to contact the electromagnet, thereby positioning the electromagnet in the magnet slot.

[0015] In a preferred solution, an annular ball groove is provided on the top surface of the wafer support platform, and a ball is provided in the ball groove, and the ball contacts the bottom surface of the wafer in a vertical magnetic field.

[0016] In a preferred embodiment, at least two evenly distributed bayonet inserts are provided on the outer wall of the culture dish, and wall-attached columns are provided on the upper top surface of the base on both sides of the wafer support platform, and bayonet inserts matching the bayonet inserts are provided on the top of the wall-attached columns.

[0017] In a preferred embodiment, at least one first wire hole is provided on the wafer support platform, at least one second wire hole is provided on the upper layer of the base, an oblique wire hole is provided on the wall-attached column, and a third wire hole is provided on the side wall of the lower layer of the base.

[0018] In a preferred embodiment, a plurality of parallel metal strips 37 are provided on the top surface of the vertical magnetic field generating sheet 5, and electrodes 36 are provided at both ends of the metal strips 37, and the electrodes 36 are connected to a power source through a wire 38;

[0019] In a preferred embodiment, a plurality of vertically cross-arranged metal strips 37 are arranged on the top surface of the vertical magnetic field generating sheet 5, and electrodes 36 are arranged at both ends of the metal strips 37, and the electrodes 36 are connected to the power supply through wires 38;

[0020] In a preferred embodiment, a plurality of magnetic blocks 39 are provided on the top surface of the vertical magnetic field generating sheet 5 .

[0021] The cell culture dish capable of applying a three-dimensional magnetic field provided by the present invention has the following beneficial effects by adopting the above structure:

[0022] (1) The purpose of applying a three-dimensional magnetic field to cells in a culture dish was achieved, making it easier to study the effects of magnetic fields on cells;

[0023] (2) The strength and direction of the magnetic field can be adjusted automatically, so that the effects of magnetic field forces of different directions and sizes on cells in the culture dish can be studied. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The present invention will be further described below in conjunction with the accompanying drawings and embodiments:

[0025] Figure 1 It is a schematic diagram of the overall structure of the present invention.

[0026] Figure 2 This is a schematic diagram of the ball structure at the top of the vertical magnetic field generating piece of the present invention.

[0027] Figure 3 It is a schematic diagram of the magnet slot structure of the present invention.

[0028] Figure 4-6 Schematic diagram of the top surface of different vertical magnetic field generating sheets of the present invention.

[0029] In the figure: culture dish cover 1, culture dish 2, bayonet insert 3, vertical magnetic field magnetic lines 4, vertical magnetic field generating sheet 5, marking line 6, fastening rod 7, threaded section 8, nut 9, non-slip handle 10, first wire hole 11, fastening rod fixing hole 12, ball 13, sheet support platform 14, bayonet 15, oblique threading hole 16, wall column 17, angular orientation marking line 18, horizontal magnetic field magnetic lines 19, electromagnet 20, magnet slot 21, scale line 22, support plate 23, second wire hole 24, inner annular fence 25, outer annular fence 26, upper layer of base 27, fence support rod 28, third wire hole 29, lower layer of base 30, fastening bolt 31, groove 32, electromagnet wire 33, bolt slot 34, arc bayonet 35, electrode 36, metal strip 37, wire 38, magnetic block 39, ball groove 40. DETAILED DESCRIPTION

[0030] Embodiment 1:

[0031] like Figure 1 In the invention, a cell culture dish capable of applying a three-dimensional magnetic field comprises a culture dish 2, a culture dish cover 1 is arranged on the top of the culture dish 2, the culture dish 2 is fixedly arranged on a vertical magnetic field generating sheet 5, and the vertical magnetic field generating sheet 5 is used to generate a magnetic field in a vertical direction, and the magnetic field lines are as follows: Figure 1 As shown in the vertical magnetic field magnetic field lines 4, a wafer support 14 is provided below the vertical magnetic field generating sheet 5, and the vertical magnetic field generating sheet 5 is movably connected to the wafer support 14. The wafer support 14 is arranged on a base, and magnet grooves 21 are provided on the bases on both sides of the wafer support 14. Electromagnets 20 are provided in the magnet grooves 21. The electromagnets 20 are used to generate a horizontal magnetic field. The magnetic field lines are shown in FIG. Figure 1 The horizontal magnetic field lines are shown as 19.

[0032] In a preferred solution, a fastening rod 7 is provided on the bottom surface of the vertical magnetic field generating sheet 5 , a fastening rod fixing hole 12 is provided at the center of the sheet support platform 14 , and the fastening rod 7 is inserted into the fastening rod fixing hole 12 .

[0033] In a preferred embodiment, the fastening rod 7 is provided with a threaded section 8, and a nut 9 is provided on the threaded section 8 of the fastening rod 7. The nut 9 is arranged on the fastening rod 7 below the bottom surface of the wafer support table 14, and an anti-slip handle 10 is provided on the outer wall of the nut 9.

[0034] In a preferred embodiment, a marking line 6 is provided on the side wall of the vertical magnetic field generating sheet 5 , and an angular orientation marking line 18 is provided on the side wall of the sheet support platform 14 .

[0035] In a preferred embodiment, at least two evenly distributed bayonet inserts 3 are provided on the outer wall of the culture dish 2, and wall-attached columns 17 are provided on the top surface of the base upper layer 27 on both sides of the wafer support platform 14, and bayonet 15 matching the bayonet inserts 3 is provided on the top of the wall-attached columns 17.

[0036] Embodiment 2:

[0037] On the basis of Example 1, the base is divided into an upper base layer 27 and a lower base layer 30. The upper base layer 27 and the lower base layer 30 are two cylindrical structures with different cross-sectional diameters, and the cross-sectional diameter of the upper base layer 27 is smaller than the cross-sectional diameter of the lower base layer 30. The wafer support table 14 is fixedly arranged on the upper base layer 27.

[0038] An inner ring fence 25 and an outer ring fence 26 are provided on the top surface of the base lower layer 30, and the inner ring fence 25 and the outer ring fence 26 are fixed on the top surface of the base lower layer 30 through fence support rods 28. A vertical support plate 23 is provided on the inner ring fence 25 and the outer ring fence 26, and an arc-shaped bayonet 35 matching the inner ring fence 25 and the outer ring fence 26 is provided at the bottom of the support plate 23. The bottom of the support plate 23 is fixed to the inner ring fence 25 and the outer ring fence 26 by the arc-shaped bayonet 35;

[0039] The magnet slot 21 is arranged on the top of the support plate 23 .

[0040] On the basis of adopting the above structure, by adjusting the position of the support plate 23 on the circular fences 25 and 26, the direction of the horizontal magnetic field lines 19 of the two horizontal magnetic fields can be adjusted. When the angle between the horizontal magnetic field lines 19 generated by the two horizontal magnetic fields is not 180°, the entire device generates a three-dimensional magnetic field, and the angle between the two magnetic fields in the horizontal direction can be adjusted arbitrarily, which is convenient for studying cell changes under different magnetic field conditions.

[0041] Embodiment 3:

[0042] like Figure 3 The magnet slot 21 is a "U"-shaped slot structure, the electromagnet 20 is fixedly arranged in the magnet slot 21, a groove 32 is arranged on the inner bottom surface of the magnet slot 21, an electromagnet wire 33 is arranged in the groove 32, and a scale line 22 is arranged on the lower edge of the outer wall of the magnet slot 21.

[0043] In a preferred embodiment, a bolt slot 34 is provided on the side wall of the magnet slot 21, and a horizontal fastening bolt 31 is provided in the bolt slot 34. The fastening end of the fastening bolt 31 passes through the bolt slot 34 and extends into the magnet slot 21 to contact the electromagnet 20, thereby realizing the positioning of the electromagnet 20 in the magnet slot 21.

[0044] Embodiment 4:

[0045] like Figure 2 On the basis of Example 1, an annular ball groove 40 is provided on the top surface of the wafer support platform 14, and a ball 13 is provided in the ball groove 40, and the ball 13 is in contact with the bottom surface of the vertical magnetic field generating sheet 5.

[0046] Embodiment 5:

[0047] On the basis of embodiments 1, 2 and 3, the wafer support platform 14 is provided with at least one first wire hole 11, the upper layer 27 of the base is provided with at least one second wire hole 24, the wall-attached column 17 is provided with an oblique wire hole 16, and the side wall of the lower layer 30 of the base is provided with a third wire hole 29.

[0048] The wire of the vertical magnetic field generating sheet 5 passes through the first wire hole 11, the second wire hole 24 and the third wire hole 29 in sequence, and the electromagnet wire 33 of the electromagnet 20 passes through the oblique wire hole 16, the second wire hole 24 and the third wire hole 29 in sequence.

[0049] The top surface of the vertical magnetic field generating sheet 5 is provided with a structure for patterning the magnetic field;

[0050] The structure can be a plurality of metal strips 37 arranged in parallel, with electrodes 36 at both ends of the metal strips 37, and the electrodes 36 are connected to a power source through a wire 38 (such as Figure 4 );

[0051] There may be a plurality of metal strips 37 arranged vertically and crosswise, with electrodes 36 at both ends of the metal strips 37, and the electrodes 36 are connected to a power source via wires 38 (such as Figure 5 );

[0052] It can also be a plurality of magnetic blocks 39 (such as Figure 6 ).

[0053] After the above-mentioned structure for patterning the magnetic field is arranged on the top surface of the vertical magnetic field generating sheet 5, the vertical magnetic field can be adjusted by observing the patterned magnetic field.

[0054] When the first two structures are adopted, the wire 38 is connected to an external power supply, the power supply voltage is 0-220V, and the power supply is direct current.

[0055] When the magnetic block 39 is used, a sputtered Fe / Ni permanent magnet alloy block array is used to generate a magnetic field.

[0056] Embodiment 6:

[0057] On the basis of Examples 1-5:

[0058] The film support table 14 and the wall-attached column 17 are made of acrylonitrile-butadiene-styrene copolymer plastic material;

[0059] The magnet slot 21 is made of polyethylene plastic material, and the entire horizontal magnetic field generating mechanism adopts a detachable structure.

[0060] The electromagnet 20 can be replaced by a magnet, which is a rectangular rare earth permanent magnet with a size of 2×2×4 cm.

[0061] The working process of the whole device is as follows:

[0062] Place the device in the cell culture operation box. Pass the fastening rod 7 at the lower end of the vertical magnetic field generating sheet 5 through the fastening rod fixing hole 12 in the center of the wafer support 14, rotate the vertical magnetic field generating sheet 5 to the designed angle, use the nut 9 to fasten the vertical magnetic field generating sheet 5 to the wafer support 14, pass the wire of the vertical magnetic field generating sheet 5 through the first wire hole 11, the second wire hole 24, and finally through the third wire hole 29 on the side wall of the lower layer 30 of the base, connect it to the external power supply, adjust the power supply size and AC / DC form to obtain the designed magnetic field;

[0063] The horizontal magnetic field generating component (i.e., the magnet slot 21 part) is symmetrically fixed on the circular fence 25 and 26 of the base. The number of magnet slots 21 is determined according to the specific experimental design. The position of the magnet slots 21 is adjusted to obtain magnetic fields in different directions. If the magnet is an electromagnet, the magnitude of the magnetic field can be adjusted. The wire is passed through the oblique wire hole 16, the second wire hole 24, and finally through the third wire hole 29 on the side wall of the lower layer 30 of the base to connect to an external power supply.

[0064] Thereafter, the glass culture dish 2 is inserted into the bayonet at the upper end of the wall-attached column 17 through the bayonet insert 3;

[0065] Add cell culture medium and cultured cells into the culture dish 2, cover the culture dish with the cover 1, and observe the growth state of the cells under the irradiation of the magnetic field.

Claims

1. A cell culture dish capable of applying a three-dimensional magnetic field, comprising a culture dish (2), characterized in that: A culture dish cover (1) is provided on the top of the culture dish (2); the culture dish (2) is fixedly mounted on a vertical magnetic field generating sheet (5); the vertical magnetic field generating sheet (5) is used to generate a magnetic field in a vertical direction; a sheet support platform (14) is provided below the vertical magnetic field generating sheet (5); the vertical magnetic field generating sheet (5) and the sheet support platform (14) are movably connected; the sheet support platform (14) is arranged on a base; magnet grooves (21) are provided on the bases on both sides of the sheet support platform (14); electromagnets (20) are provided in the magnet grooves (21); and the electromagnets (20) are used to generate a magnetic field in a horizontal direction; The base is divided into an upper base layer (27) and a lower base layer (30), the upper base layer (27) and the lower base layer (30) are two cylindrical structures with different cross-sectional diameters, and the cross-sectional diameter of the upper base layer (27) is smaller than the cross-sectional diameter of the lower base layer (30), and the wafer support platform (14) is fixedly arranged on the upper base layer (27); An inner annular fence (25) and an outer annular fence (26) are provided on the top surface of the lower layer (30) of the base. The inner annular fence (25) and the outer annular fence (26) are fixedly arranged on the top surface of the lower layer (30) of the base via fence support rods (28). A vertical support plate (23) is provided on the inner annular fence (25) and the outer annular fence (26). An arc-shaped bayonet (35) matching the inner annular fence (25) and the outer annular fence (26) is provided at the bottom of the support plate (23). The bottom of the support plate (23) is fixed to the inner annular fence (25) and the outer annular fence (26) by snapping the arc-shaped bayonet (35). The magnet slot (21) is arranged on the top of the support plate (23); A structure for patterning a magnetic field is provided on the top surface of the vertical magnetic field generating sheet (5), and the structure for patterning a magnetic field adopts a plurality of metal strips (37) arranged in parallel or a plurality of metal strips (37) arranged in a vertical and cross pattern or a plurality of magnetic blocks (39).

2. A cell culture dish capable of applying a three-dimensional magnetic field according to claim 1, characterized in that: A fastening rod (7) is provided on the bottom surface of the vertical magnetic field generating sheet (5), a fastening rod fixing hole (12) is provided at the center of the sheet support platform (14), and the fastening rod (7) is inserted into the fastening rod fixing hole (12).

3. A cell culture dish capable of applying a three-dimensional magnetic field according to claim 2, characterized in that: The fastening rod (7) is provided with a threaded section (8), the threaded section (8) of the fastening rod (7) is provided with a nut (9), the nut (9) is arranged on the fastening rod (7) below the bottom surface of the wafer support platform (14), and an anti-slip handle (10) is provided on the outer wall of the nut (9).

4. A cell culture dish that can apply a three-dimensional magnetic field according to claim 2, characterized in that: The vertical magnetic field generating sheet (5) is provided with a marking line (6) on the side wall, and the sheet support platform (14) is provided with an angular orientation marking line (18) on the side wall.

5. A cell culture dish that can apply a three-dimensional magnetic field according to claim 1, characterized in that: The magnet slot (21) is a "U"-shaped slot structure. The electromagnet (20) is fixedly arranged in the magnet slot (21). A groove (32) is provided on the inner bottom surface of the magnet slot (21). An electromagnet wire (33) is provided in the groove (32). A scale mark (22) is provided on the lower edge of the outer wall of the magnet slot (21).

6. The cell culture dish capable of applying a three-dimensional magnetic field according to claim 5, characterized in that: A bolt slot (34) is provided on the side wall of the magnet slot (21), a horizontal fastening bolt (31) is provided in the bolt slot (34), and a fastening end of the fastening bolt (31) passes through the bolt slot (34) and extends into the magnet slot (21) to contact the electromagnet (20), thereby achieving positioning of the electromagnet (20) in the magnet slot (21).

7. The cell culture dish capable of applying a three-dimensional magnetic field according to claim 5, characterized in that: An annular ball groove (40) is provided on the top surface of the sheet support platform (14), a ball (13) is provided in the ball groove (40), and the ball (13) is in contact with the bottom surface of the vertical magnetic field generating sheet (5).

8. The cell culture dish capable of applying a three-dimensional magnetic field according to claim 1, characterized in that: At least two evenly distributed bayonet inserts (3) are provided on the outer wall of the culture dish (2), and wall-attached columns (17) are provided on the top surface of the base upper layer (27) on both sides of the wafer support platform (14), and bayonet inserts (15) matching the bayonet inserts (3) are provided on the top of the wall-attached columns (17).

9. The cell culture dish capable of applying a three-dimensional magnetic field according to claim 8, characterized in that: The wafer support platform (14) is provided with at least one first wire hole (11), the upper layer (27) of the base is provided with at least one second wire hole (24), the wall-attached column (17) is provided with an oblique wire hole (16), and the side wall of the lower layer (30) of the base is provided with a third wire hole (29).

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