A dynamic cell culture device

Abstract

The present invention relates to the field of experimental instruments, and discloses a dynamic cell culture device, which includes a cell culture plate and an oscillating device, wherein the cell culture plate includes a support plate, a cell culture hole, a first drive device, a support seat, a first drive shaft, a first gear, a connecting rod, a culture medium tank, a reserved hole, and an impeller; the oscillating device includes an oscillating device body, a tray, a second drive device, a second drive shaft, a second gear, and a lever; baffles are arranged around the tray of the oscillating device, and the support plate of the cell culture plate is nested in a cavity enclosed by the baffles around the tray. The dynamic cell culture device of the present invention solves the problem that most existing cell culture devices culture cells statically and cannot simulate the dynamic environment in the body to dynamically culture cells, and has the advantages of small size, easy use, and can improve the accuracy and scientificity of experimental results.

Description

Technical Field

[0001] The present invention relates to the field of experimental instruments, and more specifically, to a dynamic cell culture device. Background Art

[0002] At present, most of the cell culture devices commonly used in laboratories are static culture devices. The culture medium is static and the cells are cultured in a static environment. In animals, tissue fluid can flow, so the cells live in a dynamic environment. The state and function of cells cultured on static cell culture devices are different from those cultured under dynamic conditions, and the experimental results may also be different from the cell detection results in animals. Therefore, it is necessary to use dynamic cell culture devices to culture cells in a dynamic environment to maintain normal cell function, differentiation, secretion, etc., and improve the accuracy and scientificity of the experimental results.

[0003] There is also an instrument on the market that can culture cells under flow conditions. However, it is mainly used to detect fluid shear force. It is a fluid shear force system. Its shortcomings are: 1. It is not suitable for various experiments and has a small scope of use; 2. The instrument is expensive, and its cell plate is also expensive and costly; 3. It requires many auxiliary devices to be connected during use, which is inconvenient to use; 4. It is large in size and is not convenient to put into a cell culture box or clean bench for use.

[0004] Since the living environment of cells in animals is dynamic, and animals are in motion in most cases, it is necessary to develop a dynamic cell culture device that is small in size, easy to use, and can simulate the real living environment of cells and combine it with the overall movement of the animal body to culture cells, thereby improving the accuracy and scientificity of experimental results. Summary of the invention

[0005] In order to overcome at least one defect (shortcoming) of the above-mentioned prior art, the present invention provides a dynamic cell culture device with small size, low cost and convenient use.

[0006] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0007] A dynamic cell culture device, characterized in that the dynamic cell culture device comprises a cell culture plate and an oscillating device:

[0008] The cell culture plate comprises a support plate, a cell culture hole, a first driving device, a support seat, a first driving shaft, a first gear, a connecting rod, a culture medium tank, a reserved hole and an impeller, wherein the first driving device is connected to the support seat, the support seat is connected to the support plate, the first driving shaft is connected to the first driving device, the first driving device drives the first gear and the connecting rod to rotate through the first driving shaft, the connecting rod penetrates the side wall of the culture medium tank and an impeller is arranged at the end of the connecting rod, and the culture medium tank is provided with a cell culture hole and a reserved hole;

[0009] The oscillating device comprises an oscillating device body, a tray, a second driving device, a second driving shaft, a second gear and a lever, wherein the second driving device is arranged inside the oscillating device body, one end of the second driving shaft is connected to the second driving device, the other end of the second driving shaft is connected to the center of the second gear, the tooth top of the second gear contacts the lever, the two ends of the lever are movably connected to the oscillating device body, and the tray is connected to the middle of the lever through a support column;

[0010] The tray of the oscillating device is provided with baffles around it, and the tray of the cell culture plate is nested in the cavity enclosed by the baffles around it. A clamping component is provided between the tray and the tray, and the clamping component is a baffle needle, one end of which is movably connected to the baffle, and the other end of which can be moved to press the tray onto the tray, and a plurality of clamping components can be provided between the tray and the tray.

[0011] The dynamic cell culture device of the present invention is small in size, low in cost, and easy to use. It simulates the real living environment of cells to culture cells, thereby improving the accuracy and scientificity of experimental results.

[0012] Furthermore, the culture medium tanks of the cell culture plate are symmetrically distributed on the support plate in a square shape, the first driving device is arranged on the upper part of the support seat, one end of the first driving shaft is connected to the first driving device, and the other end of the first driving shaft is movably connected to the support rod arranged on the support plate, and the surface of the first driving shaft is provided with external threads at equal intervals, and the external threads are meshed with the first gear fixedly arranged in the middle of the connecting rod, and the two ends of the connecting rod respectively penetrate the corresponding side walls of the culture medium tank and the two ends of the connecting rod are provided with impellers. The culture medium tanks are distributed in a square shape, which can adapt to more experimental occasions. The gear transmission method can not only realize the change of direction of the driving force, but also has the characteristics of stable transmission, high efficiency and compact structure.

[0013] Furthermore, the number of culture medium slots of the cell culture plate can be increased or decreased in even numbers according to the needs of the experiment, and at least two are symmetrically arranged on the support plate, and a cell culture well and a reserved hole are arranged in the culture medium slot. The cell culture well is used to culture adherent cells, and the reserved hole can be arranged to form a special flow channel of the culture medium in the culture medium slot by utilizing its outer shape structure, and cells can also be statically cultured in the reserved hole, or used as a control observation area for cell culture.

[0014] Furthermore, two cell culture holes and one reserved hole may be provided in the culture medium tank. The purpose of providing two cell culture holes is to culture two kinds of cells, and each cell culture hole cultures one kind of cell, so that the two kinds of cells live in the same flowing culture medium, and the interaction and influence between the two kinds of cells can be analyzed and observed. The reserved hole can be provided to form a special flow channel of the culture medium in the culture medium tank by utilizing its external shape structure, and cells can also be statically cultured in the reserved hole, or used as a control observation area for cell culture.

[0015] Furthermore, the culture medium tanks of the cell culture plate are distributed in a circular shape on the support plate, the first driving device is installed inside the support seat, the support seat is arranged above the support plate through a supporting column, one end of the first driving shaft is connected to the first driving device, the other end of the first driving shaft is provided with a first gear, the first gear is a bevel gear, one end of the connecting rod is provided with a small bevel gear, the small bevel gear is meshed with the first gear, the other end of the connecting rod passes through the corresponding culture medium tank side wall and the end is provided with an impeller, and there is a cell culture hole and a reserved hole in the culture medium tank. The culture medium tank is distributed in a circular shape, which can adapt to more experimental occasions, and the number of culture medium tanks can be increased or decreased according to the needs of the experiment, and it only needs to increase or decrease the number of connecting rods with small bevel gears at the same time. The gear transmission method can not only realize the change of direction of the driving force, but also has the characteristics of stable transmission, high efficiency and compact structure.

[0016] Furthermore, the cell culture wells of the cell culture plate are symmetrically provided with through holes on the side walls, and a detachable circular sleeve is provided inside each cell culture well. The cell culture wells of the cell culture plate are symmetrically provided with through holes on the side walls, and a detachable circular sleeve is provided inside each cell culture well. The height of the sleeve is slightly higher than the cell culture well, so that it is easy to take out from the cell culture well. The cell culture well is suitable for culturing adherent cells. In the early stage of the experiment, the sleeve is first placed in the cell culture well, so that the through holes on the side walls of the cell culture well are blocked, and the culture medium in the cell culture well is in a closed and static state, and the cells can adhere to the bottom plate inside the cell culture well. After the cells are completely adhered, the sleeve is removed, so that the culture medium in the cell culture well and the culture medium in the culture medium tank can be merged into one.

[0017] Furthermore, a rubber sealing ring is provided at the joint where the connecting rod of the cell culture plate passes through the side wall of the culture medium tank. In this way, during the rotation of the connecting rod, it can reduce the wear of the joint between the side wall of the culture medium tank and the connecting rod and eliminate noise, and it can also play a sealing role to prevent the culture medium from leaking to the outside. At the same time, it can also serve as a fulcrum of the connecting rod to ensure the dynamic balance of the connecting rod.

[0018] Furthermore, the impeller may be an open impeller, a semi-open impeller or a closed impeller. The present invention prefers a closed impeller because the closed impeller has a higher efficiency and can prevent the culture solution from splashing around, thereby ensuring that the culture medium flows smoothly under the action of the impeller.

[0019] Furthermore, the portion where the lever of the oscillating device contacts the tooth top of the second gear is a claw-shaped structure, the bottom end of the claw-shaped structure is connected to the lever, and the top end of the claw-shaped structure contacts the tooth top of the second gear.

[0020] Furthermore, both ends of the lever of the oscillation device are movably connected to the oscillation device body through a pivot structure, and the pivot structure includes a first support shaft and a second support shaft, the first support shaft is arranged at one end of the lever connected to the claw-shaped structure, and the second support shaft is arranged at the other end of the lever, one end of the first support shaft passes through the middle of the claw-shaped structure, the other end of the first support shaft is connected to the oscillation device body, the middle part of the second support shaft passes through the lever, and both ends of the second support shaft are fixedly connected to the oscillation device body.

[0021] Furthermore, the oscillation device also includes two limit rods, which are respectively arranged on both sides of the lever at the lower part of the claw-shaped structure, one end of the limit rod is fixedly connected to the oscillation device body, and the other end of the limit rod is suspended.

[0022] Furthermore, the shapes of the culture medium grooves, cell culture holes and reserved holes on the cell culture plate can be made into any shapes as long as the technical effects of the present invention can be achieved and the requirements for dynamic cell culture can be met.

[0023] Furthermore, in order to prevent the cell sample from being contaminated, the dynamic cell culture plate can be equipped with a transparent cover, and the material of the transparent cover can be made of plastic, organic glass and other materials, which is convenient for observing the cell culture state and can isolate the cell sample from the outside world to prevent the cell sample from being contaminated. The shape of the transparent cover can be made into any shape, as long as it can isolate the cell sample on the cell culture plate from the external environment, and it will not be described in detail in the present invention.

[0024] In some embodiments, the first driving device is a motor device, the motor device is fixedly connected to the support base, one side of the motor device is connected to the first driving shaft, a battery box and a power line are arranged inside the support base, the power line connects the motor and the battery box, a battery is used to provide power for the motor, and a first power switch is arranged outside the support base. The motor drive has a simple structure, a small size, is easy to use, and operates stably and permanently.

[0025] In some embodiments, the first driving device is a clockwork device, the clockwork device is fixedly connected to the support seat, one side of the clockwork device is connected to the first driving shaft, and the other side of the clockwork device is provided with a first clockwork handle. The clockwork drive does not require energy consumption, has a simple structure, and is easy to use.

[0026] In some embodiments, the second driving device is a motor device, the motor device is arranged inside the oscillating device body, one end of the second driving shaft is connected to the motor, the other end of the second driving shaft is fixedly connected to the center of the second gear, a battery box is arranged inside the oscillating device body to provide power for the motor, and a second power switch is arranged outside the body. The motor drive has a simple structure, a small size, is easy to use, and has a long-lasting and stable operation.

[0027] In some embodiments, the second driving device is a clockwork device, the clockwork device is arranged inside the oscillating device body, a second clockwork handle is arranged on one side of the clockwork device, the second clockwork handle penetrates the side wall of the oscillating device body and then emerges from the oscillating device body, and the other side of the clockwork device is fixedly connected to the center of the second gear through the second driving shaft. The clockwork drive does not require energy consumption, has a simple structure, and is easy to use.

[0028] When the dynamic cell culture device disclosed in the present invention is in use, the first driving device is started, and the first gear is driven to rotate by the first driving shaft connected to the first driving device, and the first gear then drives the connecting rod and the impeller at the end of the connecting rod to rotate. Under the action of the impeller, the culture medium will flow in the culture medium groove, so that the cells in the cell hole are in a dynamic environment, and then the second driving device is started, and the second driving device drives the second gear to rotate through the second driving shaft. During the rotation of the second gear, the tooth top of the second gear contacts with the claw structure at one end of the lever, pushing the claw structure to produce displacement, so that the lever drives the tray to swing; when the second gear continues to rotate, the tooth top of the second gear is disengaged from the claw structure at the end of the lever, and the claw structure returns to its original position. Under the action of inertia, the claw structure will continue to produce displacement in the opposite direction, and the lever drives the tray to continue to swing in the opposite direction of the previous swinging direction. In this way, the movement of the lever and the tray is similar to the principle of simple pendulum motion. In this cycle, the tray swings left and right, which plays an oscillating role on the cell culture plate placed on the tray, and realizes dynamic cell culture.

[0029] The descriptions of “first” and “second” mentioned above are for descriptive purposes only and should not be understood as indicating or suggesting their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as “first” and “second” may explicitly or implicitly include at least one such feature.

[0030] Compared with the prior art, the technical solution of the present invention has the following beneficial effects:

[0031] 1. Small size: easy to put into cell culture box, clean bench and other instruments;

[0032] 2. Easy to use: simple operation, no need to connect too many devices and AC power supply;

[0033] 3. Cultivating cells by simulating the dynamic environment in animals can improve the accuracy and scientificity of experimental results. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0035] Figure 1 is a schematic diagram of the three-dimensional structure of implementation mode 1;

[0036] Figure 2 is a schematic diagram of the three-dimensional structure of implementation mode 2;

[0037] Figure 3 It is a schematic diagram of the three-dimensional structure of implementation mode 3;

[0038] Figure 4 is a schematic diagram of the three-dimensional structure of implementation mode 4;

[0039] Figure 5 is a schematic diagram of the three-dimensional structure of implementation mode 5;

[0040] Figure 6 is a schematic diagram of the three-dimensional structure of implementation mode 6;

[0041] Figure 7 is a schematic diagram of the three-dimensional structure of implementation mode 7;

[0042] Figure 8 is a schematic diagram of the three-dimensional structure of implementation mode 8;

[0043] Fig. 9 is a schematic diagram of the three-dimensional structure of implementation mode 9;

[0044] Fig.10is a schematic diagram of the three-dimensional structure of implementation mode 10;

[0045] Fig.11 is a schematic diagram of the three-dimensional structure of implementation mode 11;

[0046] Fig.12 is a schematic diagram of the three-dimensional structure of implementation mode 12;

[0047] Fig.13 It is a schematic diagram of the three-dimensional structure of the motor-driven oscillation device;

[0048] Fig.14 for Fig.13 AA section view;

[0049] Fig.15 It is a schematic diagram of the three-dimensional structure of the spring-driven oscillation device;

[0050] Fig.16 for Fig.15 BB cross-section diagram;

[0051] Fig.17 is a schematic diagram of the connection structure between the second gear and the claw structure;

[0052] Fig.18 It is a side view of the connection structure between the first gear (large bevel gear) and the small bevel gear 152;

[0053] Fig.19 It is a bottom view of the connection structure between the first gear (large bevel gear) and the small bevel gear 152;

[0054] Fig. 20 It is a schematic diagram of the structure of the cell culture hole and the sleeve;

[0055] Fig.21 Schematic diagram of the three-dimensional structure of the impeller;

[0056] Fig. 22 for Fig.21 AA cross-section diagram.

[0057] In the figure: 11-support plate, 12-cell culture hole, 121-sleeve, 122-through hole, 13-first driving device, 131-first driving shaft, 132-support rod, 133-support seat, 134-support column, 14-first gear, 141-external thread, 15-connecting rod, 151-impeller, 152-small bevel gear, 16-culture medium tank, 161-reserved hole, 17-rubber sealing ring, 18-first spring handle, 19-first power switch;

[0058] 21-oscillation device body, 22-tray, 221-pillar, 23-second driving device, 231-second driving shaft, 24-second gear, 241-claw structure, 25-lever, 251-first supporting shaft, 252-second supporting shaft, 26-limiting rod, 27-second spring handle, 28-second power switch, 29-battery box;

[0059] 3-Clamping parts. DETAILED DESCRIPTION

[0060] The drawings are only used for illustrative purposes and should not be construed as limitations on this patent. In order to better illustrate this embodiment, some parts of the drawings may be omitted, enlarged, or reduced, and do not represent the size of the actual product. For those skilled in the art, it is understandable that some well-known structures and their descriptions in the drawings may be omitted.

[0061] The descriptions of “first” and “second” involved in the embodiments of the present invention are for descriptive purposes only and should not be understood as indicating or suggesting their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as “first” and “second” may explicitly or implicitly include at least one of such features.

[0062] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, which can be said to be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0063] In order to have a clearer understanding of the technical features, purposes and effects of the present invention, specific embodiments of the present invention are now described in detail with reference to the accompanying drawings, but the embodiments of the present invention are not limited thereto.

[0064] Implementation 1:

[0065] like Figure 1 , Fig.13 and Fig.14 As shown, a dynamic cell culture device comprises a cell culture plate and an oscillating device:

[0066] The cell culture plate includes a support plate 11, a cell culture hole 12, a first driving device 13, a support seat 133, a first driving shaft 131, a first gear 14, a connecting rod 15, a culture medium tank 16, a reserved hole 161 and an impeller 151, wherein the first driving device 13 is arranged on the upper part of the supporting seat 133, and the supporting seat 133 is arranged on the support plate 11, the first driving shaft 131 is connected to the first driving device 13, and the first driving device 13 drives the first gear 14 and the connecting rod 15 to rotate through the first driving shaft 131, the connecting rod 15 passes through the side wall of the culture medium tank 16 and the end of the connecting rod 15 is provided with an impeller 151, and the cell culture hole 12 and the reserved hole 161 are arranged inside the culture medium tank 16;

[0067] The oscillating device comprises an oscillating device body 21, a tray 22, a second driving device 23, a second driving shaft 231, a second gear 24 and a lever 25, wherein the second driving device 23 is arranged inside the oscillating device body 21, one end of the second driving shaft 231 is connected to the second driving device 23, the other end of the second driving shaft 231 is connected to the center of the second gear 24, the tooth top of the second gear 24 is in contact with the lever 25, both ends of the lever 25 are movably connected to the oscillating device body 21, and the tray 22 is connected to the middle of the lever 25 through a pillar 221;

[0068] The tray 22 of the oscillation device is provided with baffles on all sides, and the support plate 11 of the cell culture plate is nested in the cavity enclosed by the baffles on all sides of the tray 22. A clamping component 3 is provided between the tray 22 and the support plate 11. The clamping component 3 is a baffle needle, one end of which is movably connected to the baffle, and the other end of the baffle needle can be moved to press the support plate 11 onto the tray 22. A plurality of clamping components 3 are provided between the support plate 11 and the tray 22.

[0069] The culture medium tank 16 of the cell culture plate is symmetrically distributed on the support plate 11 in a square shape, one end of the first driving shaft 131 is connected to the first driving device 13, and the other end of the first driving shaft 131 is movably connected to the support rod 132 arranged on the support plate 11. The surface of the first driving shaft 131 is provided with external threads 141 at equal intervals, and the external threads 141 are meshed with the first gear 14 fixedly arranged in the middle of the connecting rod 15. The two ends of the connecting rod 15 respectively penetrate the corresponding side walls of the culture medium tank 16, and the two ends of the connecting rod 15 are provided with impellers 151. The culture medium tank 16 is distributed in a square shape, which can adapt to more experimental occasions.

[0070] The culture medium tank 16 is provided with a cell culture hole 12 and a reserved hole 161. The cell culture hole 12 is used to culture adherent cells, and the reserved hole 161 can be used to form a special flow channel for the culture medium in the culture medium tank 16 by utilizing its outer structure, and can also be used to statically culture cells in the reserved hole 161, or as a control observation area for cell culture.

[0071] The connecting rod 15 of the cell culture plate passes through the joint of the side wall of the culture medium groove 16 and is provided with a rubber sealing ring 17. In this way, during the rotation of the connecting rod 15, it can not only reduce the wear of the joint between the side wall of the culture medium groove 16 and the connecting rod 15 and eliminate noise, but also play a sealing role to prevent the culture medium from leaking to the outside. At the same time, it can also serve as a fulcrum of the connecting rod 15 to ensure the dynamic balance of the connecting rod 15.

[0072] like Fig. 20 As shown, the side walls of the cell culture wells 12 of the cell culture plate are symmetrically provided with through holes 122, and each cell culture well 12 is equipped with a detachable circular sleeve 121, and the height of the sleeve 121 is slightly higher than the cell culture well 12, so that it is easy to be taken out from the cell culture well 12. The cell culture well 12 is suitable for culturing adherent cells. In the early stage of the experiment, the sleeve 121 is first placed in the cell culture well 12, so that the through holes 122 on the side walls of the cell culture well 12 are blocked, and the culture medium in the cell culture well 12 is in a closed and static state, and the cells can adhere to the bottom plate inside the cell culture well 12. After the cells are completely attached to the wall, the sleeve 121 is removed, so that the culture medium in the cell culture well 12 and the culture medium in the culture medium tank 16 can be merged into one.

[0073] like Fig.21 and Fig. 22 As shown, the impeller 151 is preferably a closed impeller, because the closed impeller has higher efficiency and can prevent the culture solution from splashing around, thereby ensuring that the culture medium flows smoothly under the action of the impeller 151.

[0074] like Fig.13 , Fig.14 and Fig.17 As shown, the part where the lever 25 of the oscillating device contacts the tooth top of the second gear 24 is a claw structure 241 , the bottom end of the claw structure 241 is connected to the lever 25 , and the top end of the claw structure 241 contacts the tooth top of the second gear 24 .

[0075] Both ends of the lever 25 of the oscillation device are movably connected to the oscillation device body 21 through a pivot structure, and the pivot structure includes a first support shaft 251 and a second support shaft 252. The first support shaft 251 is arranged at one end of the lever 25 connected to the claw structure 241, and the second support shaft 252 is arranged at the other end of the lever 25. One end of the first support shaft 251 passes through the middle of the claw structure 241, and the other end of the first support shaft 251 is connected to the oscillation device body 21. The middle part of the second support shaft 252 passes through the lever 25, and both ends of the second support shaft 252 are fixedly connected to the oscillation device body 21.

[0076] The oscillating device further comprises two limiting rods 26 , which are respectively arranged on both sides of the lever 25 at the lower part of the claw-shaped structure 241 , one end of the limiting rod 26 is fixedly connected to the oscillating device body 21 , and the other end of the limiting rod 26 is suspended.

[0077] like Figure 1 As shown, the first driving device 13 is a motor device, one side of which is connected to a first driving shaft 131. A battery is used to provide power for the motor, and the bottom of the motor device is fixedly connected to a support base 133, a battery box and a power cord are arranged inside the support base 133, and the power cord connects the motor and the battery box, and a first power switch 19 is arranged outside the support base 133. The motor device is used for driving, which has a simple structure, a small size, is easy to use, and has a long-lasting and stable operation.

[0078] like Fig.14 As shown, the second driving device 23 is a motor device, which is arranged inside the oscillating device body 21, one end of the second driving shaft 231 is connected to the motor device, and the other end of the second driving shaft 231 is fixedly connected to the center of the second gear 24, a battery box is arranged inside the oscillating device body 21 to provide power for the motor, and a second power switch 28 is arranged outside the body. The motor device is used for driving, which has a simple structure, a small size, is easy to use, and has a long-lasting and stable operation.

[0079] When the dynamic cell culture device disclosed in the present invention is used, the first driving device 13 is started, and the first driving shaft 131 connected to the first driving device 13 drives the first gear 14 to rotate. The first gear 14 drives the connecting rod 15 and the impeller 151 at the end of the connecting rod 15 to rotate through the external thread 141. Under the action of the impeller 151, the culture medium will flow in the culture medium tank 16, so that the cells in the cell hole are in a dynamic environment. Then the second driving device 23 is started, and the second driving device 23 drives the second gear 24 to rotate through the second driving shaft 231. During the rotation of the second gear 24, the tooth top of the second gear 24 and the claw at one end of the lever 25 are engaged. The lever 25 contacts the second gear 24 and pushes the claw structure 241 to move, so that the lever 25 drives the tray 22 to swing; when the second gear 24 continues to rotate, the tooth top of the second gear 24 disengages from the claw structure 241 at the end of the lever 25, and the claw structure 241 returns to its original position. Under the action of inertia, the claw structure 241 will continue to move in the opposite direction, and the lever 25 drives the tray 22 to continue to swing in the opposite direction of the previous swinging direction. In this way, the movement of the lever 25 and the tray 22 is similar to the principle of simple pendulum motion. In this cycle, the tray 22 swings left and right, which has an oscillating effect on the cell culture plate placed on the tray 22, thereby realizing dynamic cell culture.

[0080] Implementation 2:

[0081] like Figure 1 and Figure 2 As shown, the difference between Embodiment 2 and Embodiment 1 is that the driving mode of the second driving device 23 is different.

[0082] like Figure 2 and Fig.16 As shown, the first driving device 13 is a motor device, one side of which is connected to a first driving shaft 131. A battery is used to provide power for the motor, and the bottom of the motor device is fixedly connected to a support base 133, a battery box and a power cord are arranged inside the support base 133, and the power cord connects the motor and the battery box, and a first power switch 19 is arranged outside the support base 133. The motor device is used for driving, which has a simple structure, a small size, is easy to use, and has a long-lasting and stable operation.

[0083] The second driving device 23 is a clockwork device, which is arranged inside the oscillating device body 21. A second clockwork handle 27 is arranged on one side of the clockwork device, and the second clockwork handle 27 penetrates the side wall of the oscillating device body 21 and is exposed outside the oscillating device body 21. The other side of the clockwork device is fixedly connected to the center of the second gear 24 through the second driving shaft 231. The clockwork device is driven without energy consumption, has a simple structure, and is easy to use.

[0084] Implementation 3:

[0085] like Figure 1 and Figure 3 As shown, the difference between Embodiment 3 and Embodiment 1 is that the driving mode of the first driving device 13 is different.

[0086] like Figure 3 and Fig.14 As shown, the first driving device 13 is a clockwork device, one side of which is connected to the first driving shaft 131, and the other side of which is provided with a first clockwork handle 18. The clockwork device is driven without energy consumption, has a simple structure, and is easy to use.

[0087] The second driving device 23 is a motor device, which is arranged inside the oscillating device body 21, one end of the second driving shaft 231 is connected to the motor, and the other end of the second driving shaft 231 is fixedly connected to the center of the second gear 24. A battery box 29 is arranged inside the oscillating device body 21 to provide power for the motor, and a second power switch 28 is arranged outside the body. The motor device is used for driving, which has a simple structure, a small size, is easy to use, and has a long-lasting and stable operation.

[0088] Implementation 4:

[0089] like Figure 1 and Figure 4 As shown, the difference between Embodiment 4 and Embodiment 1 is that the driving modes of the first driving device 13 and the second driving device 23 are different.

[0090] like Figure 4 and Fig.16 As shown, the first driving device 13 is a clockwork device, one side of which is connected to the first driving shaft 131, and the other side of which is provided with a first clockwork handle 18. The clockwork device is driven without energy consumption, has a simple structure, and is easy to use.

[0091] The second driving device 23 is a clockwork device, which is arranged inside the oscillating device body 21. A second clockwork handle 27 is arranged on one side of the clockwork device, and the second clockwork handle 27 penetrates the side wall of the oscillating device body 21 and is exposed outside the oscillating device body 21. The other side of the clockwork device is fixedly connected to the center of the second gear 24 through the second driving shaft 231. The clockwork device is driven without energy consumption, has a simple structure, and is easy to use.

[0092] Implementation 5:

[0093] like Figure 5 , Fig.13 and Fig.14 As shown, a dynamic cell culture device comprises a cell culture plate and an oscillating device:

[0094] The cell culture plate includes a support plate 11, a cell culture hole 12, a first driving device 13, a support seat 133, a first driving shaft 131, a first gear 14, a connecting rod 15, a culture medium tank 16, a reserved hole 161 and an impeller 151, wherein the first driving device 13 is arranged on the upper part of the supporting seat 133, and the supporting seat 133 is arranged on the support plate 11, the first driving shaft 131 is connected to the first driving device 13, and the first driving device 13 drives the first gear 14 and the connecting rod 15 to rotate through the first driving shaft 131, the connecting rod 15 passes through the side wall of the culture medium tank 16 and the end of the connecting rod 15 is provided with an impeller 151, and the cell culture hole 12 and the reserved hole 161 are arranged inside the culture medium tank 16;

[0095] The oscillating device comprises an oscillating device body 21, a tray 22, a second driving device 23, a second driving shaft 231, a second gear 24 and a lever 25, wherein the second driving device 23 is arranged inside the oscillating device body 21, one end of the second driving shaft 231 is connected to the second driving device 23, the other end of the second driving shaft 231 is connected to the center of the second gear 24, the tooth top of the second gear 24 is in contact with the lever 25, both ends of the lever 25 are movably connected to the oscillating device body 21, and the tray 22 is connected to the middle of the lever 25 through a pillar 221;

[0096] The tray 22 of the oscillation device is provided with baffles on all sides, and the support plate 11 of the cell culture plate is nested in the cavity enclosed by the baffles on all sides of the tray 22. A clamping component 3 is provided between the tray 22 and the support plate 11. The clamping component 3 is a baffle needle, one end of which is movably connected to the baffle, and the other end of the baffle needle can be moved to press the support plate 11 onto the tray 22. A plurality of clamping components 3 are provided between the support plate 11 and the tray 22.

[0097] The culture medium tank 16 of the cell culture plate is symmetrically distributed on the support plate 11 in a square shape, one end of the first driving shaft 131 is connected to the first driving device 13, and the other end of the first driving shaft 131 is movably connected to the support rod 132 arranged on the support plate 11. The surface of the first driving shaft 131 is provided with external threads 141 at equal intervals, and the external threads 141 are meshed with the first gear 14 fixedly arranged in the middle of the connecting rod 15. The two ends of the connecting rod 15 respectively penetrate the corresponding side walls of the culture medium tank 16, and the two ends of the connecting rod 15 are provided with impellers 151. The culture medium tank 16 is distributed in a square shape, which can adapt to more experimental occasions.

[0098] The culture medium tank 16 is provided with two cell culture holes 12 and a reserved hole 161. The purpose of providing the two cell culture holes 12 is to culture two types of cells, and each cell culture hole 12 cultures one type of cell, so that the two types of cells live in the same flowing culture medium, and the interaction and influence between the two types of cells can be analyzed and observed. The reserved hole 161 can be provided to form a special flow channel for the culture medium in the culture medium tank 16 by utilizing its external shape structure, and cells can also be statically cultured in the reserved hole 161, or used as a control observation area for cell culture.

[0099] The connecting rod 15 of the cell culture plate passes through the joint of the side wall of the culture medium groove 16 and is provided with a rubber sealing ring 17. In this way, during the rotation of the connecting rod 15, it can not only reduce the wear of the joint between the side wall of the culture medium groove 16 and the connecting rod 15 and eliminate noise, but also play a sealing role to prevent the culture medium from leaking to the outside. At the same time, it can also serve as a fulcrum of the connecting rod 15 to ensure the dynamic balance of the connecting rod 15.

[0100] like Fig. 20 As shown, the side walls of the cell culture wells 12 of the cell culture plate are symmetrically provided with through holes 122, and each cell culture well 12 is equipped with a detachable circular sleeve 121, and the height of the sleeve 121 is slightly higher than the cell culture well 12, so that it is easy to be taken out from the cell culture well 12. The cell culture well 12 is suitable for culturing adherent cells. In the early stage of the experiment, the sleeve 121 is first placed in the cell culture well 12, so that the through holes 122 on the side walls of the cell culture well 12 are blocked, and the culture medium in the cell culture well 12 is in a closed and static state, and the cells can adhere to the bottom plate inside the cell culture well 12. After the cells are completely attached to the wall, the sleeve 121 is removed, so that the culture medium in the cell culture well 12 and the culture medium in the culture medium tank 16 can be merged into one.

[0101] like Fig.21 and Fig. 22 As shown, the impeller 151 is preferably a closed impeller, because the closed impeller has higher efficiency and can prevent the culture solution from splashing around, thereby ensuring that the culture medium flows smoothly under the action of the impeller 151.

[0102] like Fig.13 , Fig.14 and Fig.17 As shown, the part where the lever 25 of the oscillating device contacts the tooth top of the second gear 24 is a claw structure 241 , the bottom end of the claw structure 241 is connected to the lever 25 , and the top end of the claw structure 241 contacts the tooth top of the second gear 24 .

[0103] Both ends of the lever 25 of the oscillation device are movably connected to the oscillation device body 21 through a pivot structure, and the pivot structure includes a first support shaft 251 and a second support shaft 252. The first support shaft 251 is arranged at one end of the lever 25 connected to the claw structure 241, and the second support shaft 252 is arranged at the other end of the lever 25. One end of the first support shaft 251 passes through the middle of the claw structure 241, and the other end of the first support shaft 251 is connected to the oscillation device body 21. The middle part of the second support shaft 252 passes through the lever 25, and both ends of the second support shaft 252 are fixedly connected to the oscillation device body 21.

[0104] Furthermore, the oscillation device further comprises two limiting rods 26, which are respectively arranged on both sides of the lever 25 at the lower part of the claw-shaped structure 241, one end of the limiting rod 26 is fixedly connected to the oscillation device body 21, and the other end of the limiting rod 26 is suspended.

[0105] like Figure 5 As shown, the first driving device 13 is a motor device, one side of which is connected to a first driving shaft 131. A battery is used to provide power for the motor, and the bottom of the motor device is fixedly connected to a support base 133, a battery box and a power cord are arranged inside the support base 133, and the power cord connects the motor and the battery box, and a first power switch 19 is arranged outside the support base 133. The motor device is used for driving, which has a simple structure, a small size, is easy to use, and has a long-lasting and stable operation.

[0106] like Fig.14 As shown, the second driving device 23 is a motor device, which is arranged inside the oscillating device body 21, one end of the second driving shaft 231 is connected to the motor, and the other end of the second driving shaft 231 is fixedly connected to the center of the second gear 24, a battery box 29 is arranged inside the oscillating device body 21 to provide power for the motor, and a second power switch 28 is arranged outside the body. The motor device is used for driving, which has a simple structure, a small size, is easy to use, and has a long-lasting and stable operation.

[0107] When the dynamic cell culture device disclosed in the present invention is used, the first driving device 13 is started, and the first driving shaft 131 connected to the first driving device 13 drives the first gear 14 to rotate. The first gear 14 drives the connecting rod 15 and the impeller 151 at the end of the connecting rod 15 to rotate through the external thread 141. Under the action of the impeller 151, the culture medium will flow in the culture medium tank 16, so that the cells in the cell hole are in a dynamic environment. Then the second driving device 23 is started, and the second driving device 23 drives the second gear 24 to rotate through the second driving shaft 231. During the rotation of the second gear 24, the tooth top of the second gear 24 and the claw at one end of the lever 25 are engaged. The lever 25 contacts the second gear 24 and pushes the claw structure 241 to move, so that the lever 25 drives the tray 22 to swing; when the second gear 24 continues to rotate, the tooth top of the second gear 24 disengages from the claw structure 241 at the end of the lever 25, and the claw structure 241 returns to its original position. Under the action of inertia, the claw structure 241 will continue to move in the opposite direction, and the lever 25 drives the tray 22 to continue to swing in the opposite direction of the previous swinging direction. In this way, the movement of the lever 25 and the tray 22 is similar to the principle of simple pendulum motion. In this cycle, the tray 22 swings left and right, which has an oscillating effect on the cell culture plate placed on the tray 22, thereby realizing dynamic cell culture.

[0108] Implementation 6:

[0109] like Figure 5 and Figure 6 As shown, the difference between Embodiment 6 and Embodiment 5 is that the driving mode of the second driving device 23 is different.

[0110] like Figure 6 and Fig.16 As shown, the first driving device 13 is a motor device, one side of which is connected to a first driving shaft 131. A battery is used to provide power for the motor, and the bottom of the motor device is fixedly connected to a support base 133, a battery box and a power cord are arranged inside the support base 133, and the power cord connects the motor and the battery box, and a first power switch 19 is arranged outside the support base 133. The motor device is used for driving, which has a simple structure, a small size, is easy to use, and has a long-lasting and stable operation.

[0111] The second driving device 23 is a clockwork device, which is arranged inside the oscillating device body 21. A second clockwork handle 27 is arranged on one side of the clockwork device, and the second clockwork handle 27 penetrates the side wall of the oscillating device body 21 and is exposed outside the oscillating device body 21. The other side of the clockwork device is fixedly connected to the center of the second gear 24 through the second driving shaft 231. The clockwork device is driven without energy consumption, has a simple structure, and is easy to use.

[0112] Implementation 7:

[0113] like Figure 5 and Figure 7 As shown, the difference between Embodiment 7 and Embodiment 5 is that the driving mode of the first driving device 13 is different.

[0114] like Figure 7 and Fig.14 As shown, the first driving device 13 is a clockwork device, one side of which is connected to the first driving shaft 131, and the other side of which is provided with a first clockwork handle 18. The clockwork device is driven without energy consumption, has a simple structure, and is easy to use.

[0115] The second driving device 23 is a motor device, which is arranged inside the oscillating device body 21, one end of the second driving shaft 231 is connected to the motor device, and the other end of the second driving shaft 231 is fixedly connected to the center of the second gear 24. A battery box 29 is arranged inside the oscillating device body 21 to provide power for the motor, and a second power switch 28 is arranged outside the body. The motor device is used for driving, which has a simple structure, a small size, is easy to use, and has a long-lasting and stable operation.

[0116] Implementation 8:

[0117] like Figure 5 and Figure 8 As shown, the difference between Embodiment 8 and Embodiment 5 is that the driving modes of the first driving device 13 and the second driving device 23 are different.

[0118] like Figure 8 and Fig.16 As shown, the first driving device 13 is a clockwork device, one side of which is connected to the first driving shaft 131, and the other side of which is provided with a first clockwork handle 18. The clockwork device is driven without energy consumption, has a simple structure, and is easy to use.

[0119] The second driving device 23 is a clockwork device, which is arranged inside the oscillating device body 21. A second clockwork handle 27 is arranged on one side of the clockwork device, and the second clockwork handle 27 penetrates the side wall of the oscillating device body 21 and is exposed outside the oscillating device body 21. The other side of the clockwork device is fixedly connected to the center of the second gear 24 through the second driving shaft 231. The clockwork device is driven without energy consumption, has a simple structure, and is easy to use.

[0120] Implementation 9:

[0121] like Fig. 9 , Fig.13 and Fig.14 As shown, a dynamic cell culture device comprises a cell culture plate and an oscillating device:

[0122] The cell culture plate includes a support plate 11, a cell culture hole 12, a first driving device 13, a support seat 133, a first driving shaft 131, a first gear 14, a connecting rod 15, a culture medium tank 16, a reserved hole 161 and an impeller 151, wherein the first driving device 13 is arranged inside the supporting seat 133, and the supporting seat 133 is arranged above the support plate 11 through a supporting column 134, the first driving shaft 131 is connected to the first driving device 13, and the first driving device 13 drives the first gear 14 and the connecting rod 15 to rotate through the first driving shaft 131, the connecting rod 15 passes through the side wall of the culture medium tank 16 and an impeller 151 is arranged at the end of the connecting rod 15, and the cell culture hole 12 and the reserved hole 161 are arranged inside the culture medium tank 16;

[0123] The oscillating device comprises an oscillating device body 21, a tray 22, a second driving device 23, a second driving shaft 231, a second gear 24 and a lever 25, wherein the second driving device 23 is arranged inside the oscillating device body 21, one end of the second driving shaft 231 is connected to the second driving device 23, the other end of the second driving shaft 231 is connected to the center of the second gear 24, the tooth top of the second gear 24 is in contact with the lever 25, both ends of the lever 25 are movably connected to the oscillating device body 21, and the tray 22 is connected to the middle of the lever 25 through a pillar 221;

[0124] The tray 22 of the oscillation device is provided with baffles on all sides, and the support plate 11 of the cell culture plate is nested in the cavity enclosed by the baffles on all sides of the tray 22. A clamping component 3 is provided between the tray 22 and the support plate 11. The clamping component 3 is a baffle needle, one end of which is movably connected to the baffle, and the other end of the baffle needle can be moved to press the support plate 11 onto the tray 22. A plurality of clamping components 3 are provided between the support plate 11 and the tray 22.

[0125] like Fig. 9 , Fig.18 and Fig.19 As shown, the culture medium tank 16 of the cell culture plate is distributed in a circular shape on the support plate 11, and there is a cell culture hole 12 and a reserved hole 161 in the culture medium tank 16. The first driving device 13 is arranged inside the support seat 133, and the support seat 133 is arranged above the support plate 11 through a supporting column 134. One end of the first driving shaft 131 is connected to the first driving device 13, and the other end of the first driving shaft 131 is provided with a first gear 14, and the first gear 14 is a large bevel gear. One end of the connecting rod 15 is provided with a small bevel gear 152, and the small bevel gear 152 is meshed with the first gear 14 (large bevel gear). The other end of the connecting rod 15 passes through the corresponding side wall of the culture medium tank 16 and an impeller 151 is provided at the end.

[0126] The connecting rod 15 of the cell culture plate passes through the joint of the side wall of the culture medium groove 16 and is provided with a rubber sealing ring 17. In this way, during the rotation of the connecting rod 15, it can not only reduce the wear of the joint between the side wall of the culture medium groove 16 and the connecting rod 15 and eliminate noise, but also play a sealing role to prevent the culture medium from leaking to the outside. At the same time, it can also serve as a fulcrum of the connecting rod 15 to ensure the dynamic balance of the connecting rod 15.

[0127] like Fig. 20 As shown, the side walls of the cell culture wells 12 of the cell culture plate are symmetrically provided with through holes 122, and each cell culture well 12 is equipped with a detachable circular sleeve 121, and the height of the sleeve 121 is slightly higher than the cell culture well 12, so that it is easy to be taken out from the cell culture well 12. The cell culture well 12 is suitable for culturing adherent cells. In the early stage of the experiment, the sleeve 121 is first placed in the cell culture well 12, so that the through holes 122 on the side walls of the cell culture well 12 are blocked, and the culture medium in the cell culture well 12 is in a closed and static state, and the cells can adhere to the bottom plate inside the cell culture well 12. After the cells are completely attached to the wall, the sleeve 121 is removed, so that the culture medium in the cell culture well 12 and the culture medium in the culture medium tank 16 can be merged into one.

[0128] like Fig.21 and Fig. 22 As shown, the impeller 151 is a closed impeller, because the closed impeller has a higher efficiency and can prevent the culture solution from splashing around, ensuring that the culture medium flows smoothly under the action of the impeller 151.

[0129] like Fig.13 , Fig.14 and Fig.17 As shown, the part where the lever 25 of the oscillating device contacts the tooth top of the second gear 24 is a claw structure 241 , the bottom end of the claw structure 241 is connected to the lever 25 , and the top end of the claw structure 241 contacts the tooth top of the second gear 24 .

[0130] Both ends of the lever 25 of the oscillation device are movably connected to the oscillation device body 21 through a pivot structure, and the pivot structure includes a first support shaft 251 and a second support shaft 252. The first support shaft 251 is arranged at one end of the lever 25 connected to the claw structure 241, and the second support shaft 252 is arranged at the other end of the lever 25. One end of the first support shaft 251 passes through the middle of the claw structure 241, and the other end of the first support shaft 251 is connected to the oscillation device body 21. The middle part of the second support shaft 252 passes through the lever 25, and both ends of the second support shaft 252 are fixedly connected to the oscillation device body 21.

[0131] The oscillating device further comprises two limiting rods 26 , which are respectively arranged on both sides of the lever 25 at the lower part of the claw-shaped structure 241 , one end of the limiting rod 26 is fixedly connected to the oscillating device body 21 , and the other end of the limiting rod 26 is suspended.

[0132] like Fig. 9 As shown, the first driving device is a motor device, which is installed in the support seat 133, and the first driving shaft 131 passes through the bottom of the support seat 133 and is connected to the motor device. A battery box and a power line are arranged inside the support seat 133, and a battery is used to provide power for the motor. The power line connects the motor and the battery box, and a first power switch 19 is arranged outside the support seat 133. The motor device is used for driving, which has a simple structure, a small size, is easy to use, and has a long-lasting and stable operation.

[0133] like Fig.14 As shown, the second driving device 23 is a motor device, which is arranged inside the oscillating device body 21, one end of the second driving shaft 231 is connected to the motor device, and the other end of the second driving shaft 231 is fixedly connected to the center of the second gear 24, a battery box 29 is arranged inside the oscillating device body 21 to provide power for the motor, and a second power switch 28 is arranged outside the body. The motor device is used for driving, which has a simple structure, a small size, is easy to use, and has a long-lasting and stable operation.

[0134] When the dynamic cell culture device disclosed in the present invention is used, the first driving device 13 is started, and the first gear 14 (large bevel gear) is driven to rotate through the first driving shaft 131 connected to the first driving device 13. The first gear 14 (large bevel gear) drives the connecting rod 15 and the impeller 151 at the end of the connecting rod 15 to rotate through the small bevel gear 152. Under the action of the impeller 151, the culture medium will flow in the culture medium tank 16, so that the cells in the cell hole are in a dynamic environment. Then the second driving device 23 is started, and the second driving device 23 drives the second gear 24 to rotate through the second driving shaft 231. During the rotation of the second gear 24, the tooth top of the second gear 24 and the lever 2 5, the claw-shaped structure 241 at one end of the lever 25 is pushed to move, so that the lever 25 drives the tray 22 to swing; when the second gear 24 continues to rotate, the tooth top of the second gear 24 disengages from the claw-shaped structure 241 at the end of the lever 25, and the claw-shaped structure 241 returns to its original position. Under the action of inertia, the claw-shaped structure 241 continues to move in the opposite direction, and the lever 25 drives the tray 22 to continue to swing in the opposite direction of the previous swinging direction. In this way, the movement of the lever 25 and the tray 22 is similar to the principle of simple pendulum motion. In this cycle, the tray 22 swings left and right, which plays an oscillating role on the cell culture plate placed on the tray 22, and realizes dynamic cell culture.

[0135] Implementation 10:

[0136] like Fig. 9 and Fig.10 As shown, the difference between Embodiment 10 and Embodiment 9 is that the driving mode of the second driving device 23 is different.

[0137] like Fig.10 and Fig.16 As shown, the first driving device is a motor device, which is installed in the support seat 133, and the first driving shaft 131 passes through the bottom of the support seat 133 and is connected to the motor device. A battery box and a power line are arranged inside the support seat 133, and a battery is used to provide power for the motor. The power line connects the motor and the battery box, and a first power switch 19 is arranged outside the support seat 133. The motor device is used for driving, which has a simple structure, a small size, is easy to use, and has a long-lasting and stable operation.

[0138] The second driving device 23 is a clockwork device, which is arranged inside the oscillating device body 21. A second clockwork handle 27 is arranged on one side of the clockwork device, and the second clockwork handle 27 penetrates the side wall of the oscillating device body 21 and is exposed outside the oscillating device body 21. The other side of the clockwork device is fixedly connected to the center of the second gear 24 through the second driving shaft 231. The clockwork device is driven without energy consumption, has a simple structure, and is easy to use.

[0139] Implementation 11:

[0140] like Fig. 9 and Fig.11 As shown, the difference between Embodiment 11 and Embodiment 9 is that the driving mode of the first driving device 13 is different.

[0141] like Fig.10 and Fig.16 As shown, the first driving device is a clockwork device, which is installed inside the support seat 133, the first driving shaft 131 passes through the bottom of the support seat 133 and is connected to one side of the clockwork device, and the first clockwork handle 18 passes through the top of the support seat 133 and is connected to the other side of the clockwork device. The clockwork device is used for driving, no energy consumption is required, the structure is simple, and it is easy to use.

[0142] The second driving device 23 is a motor device, which is arranged inside the oscillating device body 21, one end of the second driving shaft 231 is connected to the motor device, and the other end of the second driving shaft 231 is fixedly connected to the center of the second gear 24. A battery box is arranged inside the oscillating device body 21 to provide power for the motor, and a second power switch 28 is arranged outside the body. The motor device is used for driving, which has a simple structure, a small size, is easy to use, and has a long-lasting and stable operation.

[0143] Implementation 12:

[0144] like Fig. 9 and Fig.12 As shown, the difference between Embodiment 10 and Embodiment 9 is that the driving modes of the first driving device 13 and the second driving device 23 are different.

[0145] like Fig.10 and Fig.16 As shown, the first driving device is a clockwork device, which is installed inside the support seat 133, the first driving shaft 131 passes through the bottom of the support seat 133 and is connected to one side of the clockwork device, and the first clockwork handle 18 passes through the top of the support seat 133 and is connected to the other side of the clockwork device. The clockwork device is used for driving, no energy consumption is required, the structure is simple, and it is easy to use.

[0146] The second driving device 23 is a clockwork device, which is arranged inside the oscillating device body 21. A second clockwork handle 27 is arranged on one side of the clockwork device, and the second clockwork handle 27 penetrates the side wall of the oscillating device body 21 and is exposed outside the oscillating device body 21. The other side of the clockwork device is fixedly connected to the center of the second gear 24 through the second driving shaft 231. The clockwork device is driven without energy consumption, has a simple structure, and is easy to use.

[0147] In the figure, the description of the positional relationship is only for illustrative purposes and should not be understood as a limitation on this patent; obviously, the above embodiments of the present invention are only examples for clearly illustrating the present invention, and are not limitations on the implementation methods of the present invention. For ordinary technicians in the relevant field, other different forms of changes or modifications can be made on the basis of the above description. It is not necessary and impossible to list all the implementation methods here. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the scope of protection of the claims of the present invention.

Claims

1. A dynamic cell culture device, characterized in that: The dynamic cell culture device comprises a cell culture plate and an oscillating device: The cell culture plate comprises a support plate, a cell culture hole, a first driving device, a support seat, a first driving shaft, a first gear, a connecting rod, a culture medium tank, a reserved hole and an impeller, wherein the first driving device is connected to the support seat, the first driving shaft is connected to the first driving device, the first driving device drives the first gear and the connecting rod to rotate through the first driving shaft, the connecting rod penetrates the side wall of the culture medium tank and an impeller is arranged at the end of the connecting rod, and the cell culture hole and the reserved hole are arranged inside the culture medium tank; The oscillating device comprises an oscillating device body, a tray, a second driving device, a second driving shaft, a second gear and a lever, wherein the second driving device is arranged inside the oscillating device body, one end of the second driving shaft is connected to the second driving device, the other end of the second driving shaft is connected to the center of the second gear, and the tooth top of the second gear contacts the lever. The two ends of the lever are movably connected to the oscillating device body, and the tray is connected to the middle of the lever through a support column; The tray of the oscillating device is provided with baffles on all sides, the support plate of the cell culture plate is nested in a cavity surrounded by the baffles on all sides of the tray, and clamping components are symmetrically arranged between the tray and the support plate; The clamping component is a stopper pin, one end of which is movably connected to the baffle plates around the tray, and the other end of which can be moved to press the tray onto the tray. A plurality of clamping components can be provided between the tray and the tray. The side walls of the cell culture wells are symmetrically provided with through holes, and each cell culture well is equipped with a detachable circular sleeve, the height of the sleeve is higher than the height of the cell culture well, so as to facilitate taking out the cell from the cell culture well; The connecting rod is provided with a rubber sealing ring at the joint portion penetrating the side wall of the culture medium tank, and the impeller is a closed impeller; The part where the lever of the oscillating device contacts the tooth top of the second gear is a claw-shaped structure, the bottom end of the claw-shaped structure is connected to the lever, and the top end of the claw-shaped structure contacts the tooth top of the second gear; The two ends of the lever of the oscillating device are movably connected to the oscillating device body through a pivot structure, and the pivot structure includes a first support shaft and a second support shaft, the first support shaft is arranged at one end of the lever connected to the claw structure, and the second support shaft is arranged at the other end of the lever, one end of the first support shaft passes through the middle of the claw structure, the other end of the first support shaft is connected to the oscillating device body, the middle of the second support shaft passes through the lever, and the two ends of the second support shaft are fixedly connected to the oscillating device body; The oscillating device further comprises two limiting rods, which are respectively arranged on both sides of the lever at the lower part of the claw-shaped structure, one end of the limiting rod is fixedly connected to the oscillating device body, and the other end of the limiting rod is suspended; The cell culture plates can be set up as follows: The first type, the culture medium tank of the cell culture plate is symmetrically distributed on the support plate in a square shape, a cell culture hole and a reserved hole are arranged inside the culture medium tank, the first driving device is arranged on the upper part of the support seat, the support seat is arranged on the support plate, one end of the first driving shaft is connected to the first driving device, the other end of the first driving shaft is movably connected to the support rod arranged on the support plate, the surface of the first driving shaft is evenly spaced with external threads, the external threads are meshed with the first gear fixedly arranged in the middle of the connecting rod, and the two ends of the connecting rod respectively penetrate the corresponding culture medium tank side walls and the two ends of the connecting rod are provided with impellers; The second type, the culture medium tank of the cell culture plate is square and symmetrically distributed on the support plate, two cell culture holes and a reserved hole are arranged inside the culture medium tank, the first driving device is arranged on the upper part of the support seat, the support seat is arranged on the support plate, one end of the first driving shaft is connected to the first driving device, the other end of the first driving shaft is movably connected to the support rod arranged on the support plate, the surface of the first driving shaft is evenly spaced with external threads, the external threads are meshed with the first gear fixedly arranged in the middle of the connecting rod, and the two ends of the connecting rod respectively penetrate the corresponding culture medium tank side walls and the two ends of the connecting rod are provided with impellers; The third type, the culture medium tank of the cell culture plate is distributed in a circular shape on the support plate, a cell culture hole and a reserved hole are arranged inside the culture medium tank, the first driving device is arranged inside the support seat, the support seat is arranged above the support plate through a supporting column, one end of the first driving shaft is connected to the first driving device, the other end of the first driving shaft is provided with a first gear, the first gear is a bevel gear, one end of the connecting rod is provided with a small bevel gear, the small bevel gear is meshed with the first gear, the other end of the connecting rod passes through the corresponding culture medium tank side wall and an impeller is arranged at the end; The impeller is an open impeller, a semi-open impeller or a closed impeller.

2. The dynamic cell culture device according to claim 1, characterized in that: The first driving device is a motor device, one side of which is connected to a first driving shaft; The second driving device is a motor device, which is arranged inside the oscillation device body. One end of the second driving shaft is connected to the motor device, and the other end of the second driving shaft is fixedly connected to the center of the second gear.

3. The dynamic cell culture device according to claim 1, characterized in that: The first driving device is a motor device, one side of which is connected to a first driving shaft; the second driving device is a spring device, which is arranged inside the oscillation device body, and a second spring handle is arranged on one side of the spring device, and the second spring handle penetrates through the side wall of the oscillation device body and is exposed outside the oscillation device body, and the other side of the spring device is fixedly connected to the center of the second gear through the second driving shaft.

4. The dynamic cell culture device according to claim 1, characterized in that: The first driving device is a clockwork device, one side of the clockwork device is connected to the first driving shaft, and the other side of the clockwork device is provided with a first clockwork handle; The second driving device is a motor device, which is arranged inside the oscillation device body. One end of the second driving shaft is connected to the motor device, and the other end of the second driving shaft is fixedly connected to the center of the second gear.

5. The dynamic cell culture device according to claim 1, characterized in that: The first driving device is a clockwork device, one side of the clockwork device is connected to the first driving shaft, and the other side of the clockwork device is provided with a first clockwork handle; The second driving device is a clockwork device, which is arranged inside the oscillation device body. A second clockwork handle is arranged on one side of the clockwork device. The second clockwork handle penetrates the side wall of the oscillation device body and is exposed outside the oscillation device body. The other side of the clockwork device is fixedly connected to the center of the second gear through a second driving shaft.

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