Southern catfish sex chromosome-specific molecular markers and genetic sex identification method based on the molecular markers and parthenogenesis method

Abstract

The invention relates to a sex-specific molecular marker for catfish, a genetic sex identification method for catfish based on the molecular marker, and a production method for parthenic fish. The present invention screens for the first time the specific molecular markers of the X and Y chromosomes of the southern catfish, and the PCR identification method of the genetic sex of the southern catfish based on the molecular markers has the characteristics of high efficiency, accuracy and stability, and is applicable to laboratories and wild southern catfish in different watersheds. Economically, quickly and accurately distinguish normal female fish, normal male fish, transformed female fish, transformed male fish and super male fish, realize large-scale production of all female fish fry, significantly increase aquaculture production, reduce aquaculture costs, and improve economic benefits; In addition, a large number of single-sex fry can also be obtained for basic research.

Description

technical field

[0001] The invention belongs to the field of molecular breeding, and relates to a sex-specific molecular marker for catfish, a genetic sex identification method for catfish based on the molecular marker and a production method for parthenomorphic fish. Background technique

[0002] Fish sex determination, differentiation mechanism and sex control technology breeding are of great significance in aquaculture research and application. Many fishes are sexually dimorphic. ) and carp (Cyprinus carpio) and other females grow significantly faster than males, and the realization of all-feminization breeding through sex control technology is one of the key technologies to improve their breeding production (Chen Songlin et al., 2013; Bye and Lincoln, 1986; Shao et al. , 2014). On the contrary, males of Nile tilapia (Oreochromis niloticus), yellow catfish (Pelteobagrus fulvidraco) and channel catfish (Ictalurus punctatus) are significantly better than females in growth,...

Examples

Embodiment Construction

[0027] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0028] The present invention does not indicate the experimental method of concrete condition, generally according to routine condition, such as the condition described in molecular cloning experimental guideline (third edition, J. Sambrook et al., Huang Peitang et al. translation, Science Press, 2002) , or as recommended by the manufacturer.

[0029] 1. Obtainment of sex-specific molecular markers in catfish

[0030] 1. Genomic DNA extraction

[0031] Cut 10mg of southern catfish fin rays, put it into 600μl of lysate [10mM Tris-HCl(pH8.0)+100mM EDTA(pH8.0)+100mM NaCl+5mg / ml SDS] for homogenization, and add the final concentration to 20mg / ml Proteinase K and ribonuclease A (RnaseA) with a final concentration of 100 μg / ml were digested in a water bath at 55°C until clarified, and then 600 μl of phenol-chloroform-isoamyl alcohol (volume rati...

technical field

[0001] The invention belongs to the field of molecular breeding, and relates to a sex-specific molecular marker for catfish, a genetic sex identification method for catfish based on the molecular marker and a production method for parthenomorphic fish. Background technique

[0002] Fish sex determination, differentiation mechanism and sex control technology breeding are of great significance in aquaculture research and application. Many fishes are sexually dimorphic. ) and carp (Cyprinus carpio) and other females grow significantly faster than males, and the realization of all-feminization breeding through sex control technology is one of the key technologies to improve their breeding production (Chen Songlin et al., 2013; Bye and Lincoln, 1986; Shao et al. , 2014). On the contrary, males of Nile tilapia (Oreochromis niloticus), yellow catfish (Pelteobagrus fulvidraco) and channel catfish (Ictalurus punctatus) are significantly better than females in growth,...

Examples

Embodiment Construction

[0027] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0028] The present invention does not indicate the experimental method of concrete condition, generally according to routine condition, such as the condition described in molecular cloning experimental guideline (third edition, J. Sambrook et al., Huang Peitang et al. translation, Science Press, 2002) , or as recommended by the manufacturer.

[0029] 1. Obtainment of sex-specific molecular markers in catfish

[0030] 1. Genomic DNA extraction

[0031] Cut 10mg of southern catfish fin rays, put it into 600μl of lysate [10mM Tris-HCl(pH8.0)+100mM EDTA(pH8.0)+100mM NaCl+5mg / ml SDS] for homogenization, and add the final concentration to 20mg / ml Proteinase K and ribonuclease A (RnaseA) with a final concentration of 100 μg / ml were digested in a water bath at 55°C until clarified, and then 600 μl of phenol-chloroform-isoamyl alcohol (volume rati...

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