Method for using a device integrating colorimetric and quantitative infusion functions

Abstract

The present invention discloses a device integrating colorimetric and quantitative infusion functions and a method for using the device, characterized in that the main body of the colorimetric detection and quantitative infusion device is a precision injection pump, the piston rod and the piston sleeve of the pump constitute a colorimetric cell with a variable optical path; a light source is provided at one end of the piston rod close to the colorimetric cell; a light through hole is provided at the end of the piston sleeve, and a photoelectric receiver is provided on the light through hole; and a liquid inlet hole and a liquid outlet are provided at the end of the piston sleeve. The present invention changes the optical path of the colorimetric cell and takes and discharges liquid by moving the piston rod. When the concentration of the tested sample is moderate, the concentration of the water sample is measured at a fixed optical path; when the absorbance of the measured water sample is not within the optimal range, the optical path of the colorimetric cell is changed by continuously moving the piston rod, the absorbance of the standard sample and the water sample at different optical paths is recorded, and the optical path of the standard curve with the best value is selected to calculate the concentration of the water sample. The present invention can effectively expand the measurement range of the determination method.

Description

Technical Field

[0001] The invention relates to a colorimetric detection and quantitative infusion device of a colorimetric analysis instrument and a use method thereof, and in particular to a device integrating colorimetric and quantitative infusion functions and a use method thereof. Background Art

[0002] Spectrophotometry is a method of qualitative or quantitative analysis of a substance by measuring its absorbance of light within a certain wavelength range or at a specific wavelength. It is widely used as a detection method for analyzers due to its high sensitivity, simple operation and rapidity.

[0003] According to the requirements of photometric error, the optimal range of absorbance value is 0.2-0.8. Excessive or insufficient absorbance will affect the reliability of the measured data. Therefore, when using spectrophotometry, the absorbance of the sample to be tested is usually adjusted to a suitable value by diluting the sample or changing the size of the colorimetric cell. General analyzers use colorimetric cells with fixed optical path lengths, which cannot continuously adjust the optical path length of the colorimetric cell according to the absorbance of the sample, affecting the sensitivity and applicability of the analyzer. Summary of the invention

[0004] In view of the above problems, the present invention provides a convenient and practical colorimetric detection and quantitative infusion device and a method of using the same.

[0005] The specific technical solutions of the present invention are as follows:

[0006] A method for using a device that integrates colorimetric and quantitative infusion functions, characterized in that the main body of the device is a precision injection pump, the piston rod and piston sleeve of the pump constitute a colorimetric cell with a variable optical path; the precision injection pump comprises a screw motor, a reset optical coupler, the screw of the motor is connected to the end of the piston rod without a light source through a screw nut, the screw nut is fixedly connected to the piston rod, and the reset optical coupler is used to reset the piston rod to reset the optical path of the colorimetric cell to zero; the piston rod of the pump is hollow, a light source is provided at one end close to the colorimetric cell, and is sealed with a light-transmitting material; a light through hole is provided at the end of the piston sleeve, and a photoelectric receiver is provided on the light through hole; a liquid inlet hole and a liquid outlet hole are respectively provided on both sides of the end of the piston sleeve, and the lower edge position of the hole position should not be lower than the lowest surface of the photoelectric receiver, and the liquid inlet hole and the liquid outlet hole should be Used in conjunction with a valve; the device integrates photometric detection and quantitative infusion functions. When in use, the device is used in conjunction with an automatic control system to control the motor to drive the piston rod to move according to corresponding instructions, and read the optical path and photovoltage of the colorimetric device in real time, and finally calculate the concentration value of the water sample to be tested according to the absorbance and the standard curve, including the following two situations: S1: When the concentration of the water sample to be tested is moderate, the optical path of the colorimetric device is fixed, the absorbance of the standard sample and the water sample at the fixed optical path is measured, and the concentration of the substance to be tested in the water sample is calculated by the absorbance; S2: When the absorbance of the water sample to be tested is not within the optimal range, the piston rod is moved, and the absorbance of the standard sample and the water sample at different optical path lengths is recorded in stages or continuously, and the standard curve and absorbance at the optical path length with the best standard curve are selected to calculate the concentration of the water sample to be tested.

[0007] Preferably, the reference solution used in the absorbance calculation may be distilled water or a single solution containing a low concentration of a reaction product.

[0008] Preferably, the reference solution may also be a solution after reaction with a low-concentration standard sample or a solution that has not reacted after adding reagents.

[0009] The present invention has the following beneficial effects:

[0010] The present invention is suitable for measuring water samples in different concentration ranges, and is particularly suitable for water samples with a large concentration range change. It can effectively improve the sensitivity of the instrument, reduce the determination lower limit of the analysis method, and expand the measurement range of the instrument. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] The present invention is further described below in conjunction with the accompanying drawings.

[0012] Figure 1 It is a structural schematic diagram of the present invention;

[0013] Figure 2 for Figure 1 sectional view of

[0014] Figure 3A schematic diagram of the device of the present invention used in conjunction with a colorimetric analysis analyzer;

[0015] In the figure: 1-piston rod; 2-piston sleeve; 3-colorimetric cell; 4-light source; 5-light hole; 6-photoelectric receiver; 7-liquid inlet; 8-liquid outlet; 9-reset optical coupler; 10-single-way valve; 11-cleaning solution; 12-water sample; 13-standard solution 1; 14-standard solution 2; 15-reagent 1; 16-reagent 2; 17-waste liquid; 18-air; 19-automatic control system; 20-reaction cell; 21-multi-way valve. DETAILED DESCRIPTION

[0016] Embodiment 1:

[0017] like Figure 1 , Figure 2 As shown, a device integrating colorimetry and quantitative infusion functions and a method of using the device of the present invention, the main body of the device is a precision injection pump, the piston rod 1 and the piston sleeve 2 of the pump constitute a colorimetric cell 3 with a variable optical path; the piston rod 1 is hollow, and a light source 4 is provided at one end close to the colorimetric cell 3, and is sealed with a light-transmitting material; a light hole 5 is provided at the end of the piston sleeve 2, and a photoelectric receiver 6 is provided on the light hole 5; a liquid inlet hole 7 and a liquid outlet hole 8 are respectively provided on both sides of the end of the piston sleeve 2, and the lower edge position of the hole position should not be lower than the lowest surface of the photoelectric receiver 6.

[0018] like Figure 3 As shown, the device extracts the standard sample / water sample or reagent into the colorimetric cell 3 from the valve port corresponding to the multi-way valve. After the extraction, the multi-way valve 21 turns to the valve port corresponding to the reaction cell 20. The device then pushes the standard sample / water sample or reagent taken from the colorimetric cell to the reaction cell 20 for reaction. After the reaction is completed, the automatic control system issues an instruction to automatically clean the device. After cleaning, it is reset to the optical path L=0 of the colorimetric cell. When resetting, the multi-way valve 21 turns to the waste liquid port 18, the single-way valve 10 is closed, and the piston rod 1 is pushed to L=0. After resetting, the multi-way valve turns to the port corresponding to the reaction cell 20, the single-way valve 10 is closed, and the colorimetric device extracts the reacted solution from the reaction cell 20. When extracting the sample liquid, the piston rod 1 moves downward until the colorimetric cell 3 is filled with the solution.

[0019] When the absorbance value of the measured water sample is within the optimal range, according to the optical path L set by the automatic control system 19, the piston rod pushes the excess sample liquid out of the colorimetric cell 3 from the waste liquid port 18 of the multi-way valve 21 until the optical path of the colorimetric cell 3 is equal to the optical path L set by the automatic control system 19, and the automatic control system records the photovoltage value U measured by the device under the optical path L; under the same conditions and optical path L, the photovoltage U0 is recorded with distilled water as the reference liquid, and the concentration value of the measured water sample is calculated according to the following formula.

[0020]

[0021] In the formula: C2—concentration value of standard 2;

[0022] C1—standard concentration value;

[0023] A2—absorbance value of the second marker, A2=log( ), U2 is the photovoltage value measured at the second marker of the optical path L;

[0024] A1—absorbance value of the first mark, A1=log( ), U1 is the photovoltage value measured at the first mark of the optical path L;

[0025] A—absorbance value of sample solution, A=log();

[0026] C—Concentration value of water sample.

[0027] Embodiment 2:

[0028] When the absorbance of the water sample to be tested is not within the optimal range, the piston rod is continuously moved to record the absorbance of the standard sample and the water sample at different optical path lengths in segments or continuously, and the standard curve and absorbance at the optical path length with the best standard curve are selected to calculate the concentration of the water sample to be tested.

[0029] Embodiment three:

[0030] According to Example 1, the difference of this embodiment is that: when the concentration of the measured water sample is low, after the colorimetric cell is filled with solution or distilled water, the precision syringe pump is pushed according to the set number of steps, and the photovoltage value of the solution under different optical path lengths is recorded at the same time, and the corresponding absorbance is calculated, and the absorbance under the optimal optical path length of the standard curve is selected to calculate the concentration of the measured water sample according to the formula of Example 1.

[0031] Embodiment 4:

[0032] According to Example 1 and Example 2, the difference of this embodiment is that the selected reference solution is a standard sample of a reaction product with a low concentration.

[0033] Embodiment five:

[0034] According to Example 1 and Example 2, the difference of this embodiment is that the selected reference solution is a solution after the reaction of the low-concentration standard sample.

[0035] Embodiment six:

[0036] According to Example 1 and Example 2, the difference of this embodiment is that the selected reference solution is a solution of the low-concentration standard sample after the reagent is added but not reacted.

Claims

1. A method for using a device integrating colorimetry and quantitative infusion functions, characterized in that: The main body of the device is a precision injection pump, and the piston rod and piston sleeve of the pump constitute a colorimetric cell with a variable optical path; the precision injection pump includes a screw motor, a reset optical coupler, and the screw of the motor is connected to the end of the piston rod without a light source through a screw nut, and the screw nut is fixedly connected to the piston rod, and the reset optical coupler is used to reset the piston rod to reset the optical path of the colorimetric cell to zero; the piston rod of the pump is hollow, and a light source is provided at one end close to the colorimetric cell and sealed with a light-transmitting material; a light through hole is provided at the end of the piston sleeve, and a photoelectric receiver is provided on the light through hole; a liquid inlet and a liquid outlet are respectively provided on both sides of the end of the piston sleeve, and the lower edge position of the hole should not be lower than the lowest surface of the photoelectric receiver, and the liquid inlet and outlet should be used in conjunction with a valve; the device collects light The device integrates measurement and quantitative infusion functions. When in use, it is used in conjunction with an automatic control system to control the motor to drive the piston rod to move according to corresponding instructions, and read the optical path and photovoltage of the colorimetric device in real time, and finally calculate the concentration value of the water sample to be tested according to the absorbance and the standard curve, including the following two situations: S1: When the concentration of the water sample to be tested is moderate, the optical path of the colorimetric device is fixed, the absorbance of the standard sample and the water sample at the fixed optical path is measured, and the concentration of the substance to be tested in the water sample is calculated by the absorbance; S2: When the absorbance of the water sample to be tested is not within the optimal range, the piston rod is moved, the absorbance of the standard sample and the water sample at different optical path lengths is recorded in stages or continuously, and the standard curve and absorbance at the optical path length with the best standard curve are selected to calculate the concentration of the water sample to be tested.

2. The method for using the device integrating colorimetry and quantitative infusion functions according to claim 1, characterized in that: The reference solution used in the absorbance calculation can be distilled water or a single solution containing a low concentration of reaction product.

3. The method for using the device integrating colorimetry and quantitative infusion functions according to claim 1, characterized in that: The reference solution can also be a solution after a low concentration standard sample reacts or a solution that has not reacted after adding reagents.

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