A cytological sub-magnetic effect experimental system

Abstract

The present invention relates to a cytological sub-magnetic effect experimental system, including a sub-magnetic experimental component and a control component, wherein the sub-magnetic experimental component includes an end cover, an experimental component sample rack, a cylindrical tubular coil, a magnetic isolation component, a convection fan and a slip ring; the slip ring and the convection fan are fixed to one end of the magnetic isolation component; the cylindrical tubular coil is installed inside the magnetic isolation component; the experimental component sample rack is installed inside the cylindrical tubular coil through a track inside the cylindrical tubular coil; the end cover is buckled at the other end of the magnetic isolation component; the control component is used to establish an experimental environment for the control sample group of the experiment, including a cylinder cover, a control component sample rack, a track, a transmission block, and a cylinder; the track is bonded inside the cylinder, the transmission block is fixed at both ends of the outer surface of the cylinder, the control component sample rack is installed on the track, and the cylinder cover is buckled at the open end of the cylinder. The present invention can establish a sub-magnetic environment, realize automatic control of the cell culture environment, and support the ground to carry out sub-magnetic composite environment cytological effect research.

Description

Technical Field

[0001] The invention belongs to the technical field of cell biology, and in particular relates to a cytological submagnetic effect experimental system. Background Art

[0002] As people's exploration of space continues to deepen, the cytological effects of the space environment, especially the research on the cytological compound effects of the space environment, has attracted more and more attention. However, due to the limited opportunities for real-time flight experiments in space and the extremely limited experimental resources, conducting simulation research on related space compound effects on the ground has become a necessary way for related disciplines to explore the mysteries of space and accumulate space knowledge.

[0003] Conducting research on cytological complex effects related to space environment on the ground requires the support of necessary simulation equipment, but there is currently no cytological effects research platform with complete functions, especially no platform with the ability to establish different levels of low gravity and hypomagnetic complex environments.

[0004] At present, the simulation research of space microgravity effect often uses a series of rotators (Rotate Wall Vessels, RWVs) produced in the United States, while the research on submagnetic cytology effect uses shielding boxes, etc. The technical defects of using these independent and unrelated equipment to carry out space composite effect research include:

[0005] 1) The experimental mode using the above-mentioned independent equipment cannot effectively carry out experiments on the effects of the hypomagnetic environment, which limits the research on the cytological effects of hypomagnetism on the ground.

[0006] 2) The above-mentioned existing equipment does not have an integrated cell culture environment automatic control system and cannot independently maintain the conditions required for cell culture experiments, which restricts the long-term development of related experiments. Summary of the invention

[0007] The purpose of the present invention is to provide a cytological hypomagnetic effect experimental system, establish a hypomagnetic environment, and realize automatic control of the cell culture environment, so as to support the terrestrial hypomagnetic environment cytological effect research.

[0008] The present invention provides a cytology hypomagnetic effect experimental system, comprising a hypomagnetic experimental component and a control component. The hypomagnetic experimental component is used to establish a hypomagnetic environment with a low magnetic field strength that meets the experimental requirements in the area where the cell sample is located, and comprises an end cover, an experimental component sample rack, a cylindrical tubular coil, a magnetic isolation component, a convection fan and a slip ring; the slip ring and the convection fan are fixed to one end of the magnetic isolation component; the cylindrical tubular coil is installed inside the magnetic isolation component; the experimental component sample rack is installed inside the cylindrical tubular coil through a track inside the cylindrical tubular coil; the end cover is buckled on the other end of the magnetic isolation component;

[0009] The control component is used to establish an experimental environment for the control sample group of the experiment, and includes a cylinder cover, a control component sample rack, a track, a transmission block, and a cylinder; the track is bonded to the inside of the cylinder, the transmission block is fixed at both ends of the outer surface of the cylinder, the control component sample rack is installed on the track, and the cylinder cover is buckled at the open end of the cylinder.

[0010] Furthermore, the end cover is composed of an inner cover, a magnetic isolation plate and an outer cover, and the inner cover and the outer cover are respectively bonded to two sides of the magnetic isolation plate;

[0011] The experimental component sample rack is used to install the experimental sample, and includes a main structure and a buffer, and the buffer is bonded to the main structure;

[0012] The cylindrical tubular coil is used to generate a uniform magnetic field required by the experiment, and includes a track, a support tube, and an enameled wire spirally wound outside the support tube, wherein the track is bonded to the inner surface of the support tube;

[0013] The magnetic isolation component is used to isolate the magnetic field in the experimental environment, and includes a magnetic isolation layer, a protective outer layer, a protective inner layer and a transmission block. The magnetic isolation layer is sleeved outside the protective inner layer, the protective outer layer is sleeved outside the magnetic isolation layer, and the transmission block is fixedly connected to both ends of the protective outer layer.

[0014] Furthermore, the system also includes an outer shell, which is a box structure. The submagnetic experimental component and the control component are arranged in the outer shell, and a closed cell experimental area is formed in the outer shell.

[0015] Furthermore, the shell is composed of a main body structure, an upper cover, a back cover, a hinge and a thermal insulation layer. The main body structure and the upper cover are connected by the hinge, the main body structure and the back cover are connected by screws, and the thermal insulation layer is installed inside the main body structure.

[0016] Furthermore, the system also includes a cell culture environment control component and a control system; the cell culture environment control component is used to establish the temperature and CO2 concentration conditions required for the cell experiment in the cell experiment area; the control system is used to regulate the cell culture environment control component to work according to the experimental requirements.

[0017] Furthermore, the cell culture environment control component includes a temperature control component and a CO2 concentration control component, the CO2 concentration control component includes a CO2 gas cylinder, a pressure reducing valve, a solenoid valve, a CO2 concentration sensor and a pressure pipe, the pressure reducing valve is installed on the CO2 gas cylinder, the pressure reducing valve and the solenoid valve are connected through the pressure pipe, and the CO2 concentration sensor is arranged in the cell experiment area inside the shell; the temperature control component is used to adjust the temperature of the cell experiment area, and includes a heater, a convection fan and a temperature sensor, and the temperature sensor is arranged in the cell experiment area inside the shell.

[0018] Furthermore, the control system includes a control circuit board and a display screen, the control circuit board is installed on the back cover of the shell, the display screen is installed on the upper cover of the shell, and the control circuit board is electrically connected to the cell culture environment control component and the display screen.

[0019] Through the above scheme, through the cytological sub-magnetic effect experimental system, it is possible to establish a sub-magnetic environment and realize automatic control of the cell culture environment, supporting the ground-based cytological effect research on the sub-magnetic environment.

[0020] The above description is only an overview of the technical solution of the present invention. In order to more clearly understand the technical means of the present invention and implement it according to the contents of the specification, the following is a detailed description of the preferred embodiments of the present invention in conjunction with the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the structure of the submagnetic experimental assembly of the present invention;

[0022] Figure 2 It is a schematic diagram of the sample rack structure of the present invention;

[0023] Figure 3 It is a schematic diagram of the structure of the control component of the present invention;

[0024] Figure 4 It is a schematic diagram of the shell structure of the present invention;

[0025] Figure 5 The present invention is a cytological effect experimental system integrated with a low-gravity experimental component;

[0026] Figure 6 It is a schematic diagram of the structure of the low gravity experiment assembly of the present invention;

[0027] Figure 7 It is a schematic diagram of the structure of the basic components of the present invention;

[0028] Figure 8 It is a schematic diagram of the structure of the transmission component of the present invention.

[0029] Numbers in the figure:

[0030] 1-hypermagnetic experimental assembly; 11-end cover; 12-experimental assembly sample rack; 121-main structure; 122-buffer; 13-cylindrical tubular coil; 14-magnetic isolation component; 15-convection fan; 16-slip ring;

[0031] 2-control component; 21-cylinder cover; 22-control component sample rack; 23-track; 24-transmission block; 25-cylinder;

[0032] 3-low gravity experiment assembly; 31-basic component; 311-bottom plate; 312-side plate; 313-active support frame; 314-driven support frame; 315-bearing; 32-transmission component; 321-motor; 322-movable seat; 323-active pulley; 324-synchronous belt; 325-driven pulley; 326-active rotating end; 327-driven rotating end; 33-sample tray;

[0033] 4-housing; 41-body structure; 42-upper cover; 43-back cover; 44-hinge; 45-insulation layer;

[0034] 5-Control system;

[0035] 6-Cell culture environment control components. DETAILED DESCRIPTION

[0036] The specific implementation of the present invention is further described in detail below in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0037] Ginseng Figure 1 As shown, this embodiment provides a cytological sub-magnetic effect experimental system, including a sub-magnetic experimental component 1 and a control component 2, wherein the sub-magnetic experimental component is used to establish a sub-magnetic environment with a low magnetic field strength that meets the experimental requirements in the area where the cell sample is located, and the control component is used to establish an experimental environment for the control sample group of the experiment.

[0038] The sub-magnetic experimental assembly 1 is composed of an end cover 11, an experimental assembly sample holder 12, a cylindrical tubular coil 13, a magnetic isolation component 14, a convection fan 15, and a slip ring 16. Among them, the slip ring 16 and the convection fan 15 are fixed to one end of the magnetic isolation component 14 by screws; the cylindrical tubular coil 13 is installed inside the magnetic isolation component 14; the experimental assembly sample holder 12 is installed inside the cylindrical tubular coil 13 through the track inside the cylindrical tubular coil 13, and is used to install the experimental sample; the end cover 11 is buckled on the other end (open end) of the magnetic isolation component 14, so that the magnetic isolation component 14 forms a closed space inside.

[0039] The magnetic isolation component 14 is used to isolate the magnetic field in the experimental environment, including a magnetic isolation layer, a protective outer layer, a protective inner layer and a transmission block. The magnetic isolation layer is inserted outside the protective inner layer, the protective outer layer is inserted outside the magnetic isolation layer, and the transmission block is welded to both ends of the protective outer layer. The cylindrical tubular coil 13 is used to generate a uniform magnetic field required by the experiment, including a track, a support tube, and an enameled wire spirally wound outside the support tube. The track is bonded to the inner surface of the support tube. The end cover 11 is composed of an inner cover, a magnetic isolation plate and an outer cover. The inner cover and the outer cover are respectively bonded to the two sides of the magnetic isolation plate.

[0040] Ginseng Figure 2 As shown, the experimental component sample rack 12 includes a main structure 121 and a buffer member 122 , and the buffer member 122 is bonded to the main structure 121 .

[0041] Ginseng Figure 3 As shown, the control assembly 2 includes a cylinder cover 21, a control assembly sample rack 22, a track 23, a transmission block 24, and a cylinder 25. The track 23 is bonded inside the cylinder 25, the transmission block 24 is welded to both ends of the outer surface of the cylinder 25, the control assembly sample rack 22 is installed on the track 23, and the cylinder cover 21 is buckled at the open end of the cylinder 25.

[0042] Ginseng Figure 4 As shown, the housing 4 is the installation base and protective structure of all the above components. The housing is a box structure. The submagnetic experimental component 1 and the control component 2 can be arranged in the housing 4, and a closed cell experimental area is formed in the housing 4. Specifically, the housing 4 is composed of a main body structure 41, an upper cover 42, a rear cover 43, a hinge 44 and a thermal insulation layer 45. The main body structure 41 is connected to the upper cover 42 by a hinge 44, the main body structure 41 is connected to the rear cover 43 by screws, and the thermal insulation layer 45 is installed inside the main body structure 41.

[0043] Ginseng Figure 5 As shown, in order to realize automatic control of the cell culture environment, a control system 5 and a cell culture environment control component 6 may also be provided.

[0044] Specifically, the cell culture environment control component 6 includes a temperature control component and a CO2 concentration control component. The CO2 concentration control component includes a CO2 cylinder, a pressure reducing valve, a solenoid valve, a CO2 concentration sensor and a pressure pipe. The pressure reducing valve is installed on the CO2 cylinder. The pressure reducing valve and the solenoid valve are connected by a pressure pipe. The CO2 concentration sensor is installed on the bottom plate 311 of the base component 31. After the pressure of the CO2 gas in the CO2 cylinder is reduced by the pressure reducing valve, it flows into the solenoid valve through the pressure pipe. The control system 5 adjusts the opening of the solenoid valve. After the solenoid valve is opened, the CO2 gas flows into the cell experimental area surrounded by the base component 31 through the pipeline. After the CO2 concentration sensor detects that the CO2 concentration meets the experimental requirements, the control system 5 adjusts the solenoid valve to close, and the CO2 gas is cut off and stops flowing into the cell experimental area surrounded by the base component 31. The temperature control component consists of a heater, a convection fan and a temperature sensor, which is used to adjust the temperature of the cell experimental area.

[0045] The control system 5 is composed of several control circuit boards and display screens. The control circuit boards are installed on the back (rear cover) of the shell 4, and the display screen is installed on the upper cover of the shell 4. The control circuit boards are connected to the cell culture environment control components and the display screen through wires.

[0046] As shown in 5, in order to simultaneously realize the simulation of low gravity and hypomagnetic composite environment and the automatic control of cell culture environment, a low gravity experiment component 3 can also be integrated on the basis of the cytological hypomagnetic effect experimental system. Through this system, experiments can be carried out independently without the support of other peripheral equipment, and the automatic control of the cell culture environment can be realized, supporting the research on the cytological effects of low gravity and hypomagnetic composite environment in space on the ground.

[0047] Ginseng Figure 6 As shown, the low gravity experiment assembly 3 includes a base component 31, four sets of transmission components 32 (which can be increased or decreased according to experimental requirements) and a sample tray 33. The transmission component 32 is installed in the base component 31, and the sample tray 33 is installed on the upper part of the transmission component 32. The base component 31 is the installation base of the low gravity experiment assembly 3, and is used to support the transmission component 32 and the sample tray 33 in the low gravity experiment assembly 3. The transmission component 32 is used to drive the submagnetic experiment assembly 1 and the control assembly 2 to rotate according to the experimental requirements.

[0048] Ginseng Figure 7As shown, the basic component 31 includes a bottom plate 311, a side plate 312, an active support frame 313, a driven support frame 314, and a bearing 315. The basic component 31 is composed of the bottom plate 311, the side plate 312, the active support frame 313, and the driven support frame 314 to form a rectangular box structure with an open upper end. The bottom plate 311 is arranged on the bottom surface of the rectangular box structure, the active support frame 313 and the driven support frame 314 are arranged on a group of opposite sides of the rectangular box, and the two side plates 312 are arranged on another group of opposite sides of the rectangular box. The active support frame 313 and the driven support frame 314 are provided with a plurality of bearing mounting holes, and the bearing 315 is installed in the bearing mounting holes on the active support frame 313 and the driven support frame 314.

[0049] Ginseng Figure 8 As shown, the transmission component 32 includes a motor 321, a movable seat 322, a driving pulley 323, a synchronous belt 324, a driven pulley 325, a driving rotating end 326 and a driven rotating end 327. The output end of the motor 321 is fixed to the movable seat 322 by screws, the driving pulley 323 is installed on the output shaft of the motor 321, the movable seat 322 with the motor 321 and the driving pulley 323 is fixed to the active support frame 313 by screws, and the active rotating end 326 passes through the bearing of the active support frame 313 through its end shaft. The driving pulley 323 is installed on the end shaft of the active rotating end 326, and the driven rotating end 327 passes through the inner hole of the bearing of the driven supporting frame 314 through its end shaft and is installed on the driven supporting frame 314. The synchronous belt 324 is installed between the driving pulley 323 and the driven pulley 325. The end faces of the active rotating end 326 and the driven rotating end 327 are both provided with sliding grooves that match the transmission blocks on the sub-magnetic experimental component 1 and the control component 2, so that the sub-magnetic experimental component 1 and the control component 2 can be driven to rotate.

[0050] The heater and convection fan in the cell culture environment control component 6 are installed on the driven support frame 314, and the temperature sensor is installed in the middle of the base plate 311. The control circuit board in the control system is electrically connected to the gravity experiment low component 3.

[0051] In a specific embodiment, the inner cover and the outer cover of the end cover 11 are both made of ABS material, the outer cover has a diameter of 120mm and a thickness of 5mm, the inner cover has a diameter of 85mm and a thickness of 5mm; the magnetic isolation plate is made of 1J85 material and is in the shape of a disc, with a diameter of 85mm and a thickness of 1mm. 502 glue is used to coaxially bond the outer cover, the magnetic isolation plate and the inner cover in sequence.

[0052] The main structure 121 of the experimental component sample rack 12 is formed by 3D printing of resin materials, and can be installed with 2 T25 cell culture bottles (the installation requirements of experimental samples of other specifications can be achieved by adjusting the size of the main structure). The silk surface of nylon hook and loop is used as a cushion layer (buffer 122), which is cut and pasted on the inner surface of the main structure 121 with 502 glue to ensure that the T25 cell culture bottle will not slip when the experimental component sample rack 12 moves. The control component sample rack 22 can adopt the same structure as the experimental component sample rack 12, which will not be described in detail here.

[0053] The supporting tube of the cylindrical tubular coil 13 is made of organic glass material, with an inner diameter of 85mm, a wall thickness of 2mm, a length of 400mm, and a spirally wound layer of 3mm diameter enameled wire on the outer surface (multiple layers of enameled wire of other diameters can also be used); the grooved track is machined from ABS material and adhered to the inner surface of the supporting tube with 502 glue, and the track and the conduction block are installed on the same plane.

[0054] The magnetic isolation layer of the magnetic isolation component 14 is made of 1mm thick 1J85 plate bent into a round tube and welded by argon arc welding. The inner diameter of the magnetic isolation layer is 95mm and the length is 400mm (it can also be bent into multiple magnetic isolation layers as needed); the protective outer layer is made of aluminum alloy 2A12 material and is machined, with an inner diameter of 104mm, an outer diameter of 120mm, and a length of 430mm; the protective inner layer is made of aluminum alloy 2A12 material and is machined, with an inner diameter of 95mm, an outer diameter of 102mm, and a length of 400mm; the transmission block is made of aluminum alloy 2A12 material and is machined, 20mm long and 10mm thick, and is welded to the two ends of the outer surface of the protective outer layer by argon arc welding.

[0055] The convection fan 15 adopts a 40406 series ultra-quiet convection fan with an overall size of 40mm×40mm×6mm and an operating voltage of 12DCV. It is fixed to one end of the protective outer layer of the magnetic isolation component 14 with four M3 screws.

[0056] The slip ring 16 is a 4-way cylindrical micro slip ring with a diameter of 5 mm and a length of 11.4 mm, 2 of which are used to power the cylindrical tubular coil 13 and 2 of which are used to power the convection fan 15. The slip ring 16 is bonded to the center of one end of the protective outer layer of the magnetic isolation component 14 with 502 glue.

[0057] The cylinder 25 of the control component 2 is machined from ABS material, with an inner diameter of 85 mm, an outer diameter of 95 mm, and a length of 400 mm; the internal track 23 is also machined from ABS material, with a length of 400 mm, and is bonded to the inside of the cylinder 25 with 502 glue; the transmission block 24 is made of ABS, machined, 20 mm long, 10 mm thick, and is bonded to both ends of the inner and outer surfaces of the cylinder 25 with 502 glue, and the track 23 and the transmission block 24 are on the same plane.

[0058] In the basic component 31 of the low-gravity experiment assembly 3, the active support frame 313 and the driven support frame 314 are both made of aluminum alloy 2A12 by machining, with an outer size of 490mm×200mm×30mm, an inner hollow area size of 480mm×200mm×20mm, and 8 bearing holes distributed on the upper part, with a hole diameter of There are 8 rolling bearings 315 on each of the active support frame 313 and the passive support frame 314. The outer diameter of the bearing 315 is 20mm, the inner diameter is 8mm, and the thickness is 5mm. The bottom plate 311 is made of 2A12 material, with a length of 510mm, a width of 490mm, and a thickness of 10mm. The two side plates 312 are both machined from 2A12 and have an outer dimension of 510mm×200mm×3mm. The active support frame 313 and the passive support frame 314 are each fixed to the two ends of the bottom plate 311 with 4 M4 screws, and the two side plates 312 are fixed to the sides of the active support frame 313 and the passive support frame 314 with M3 screws.

[0059] The motor 321 of the transmission component 32 is a 24V DC motor with an outer diameter of 38mm and a length of 60mm; the movable seat 322 is machined from aluminum alloy 2A12, with an outer diameter of 50mm, a length of 30mm and an inner diameter of 38mm; the active pulley 323 is machined from aluminum alloy 2A12, with 24 T-shaped teeth, a pitch circle diameter of 28mm and a thickness of 10mm; the driven pulley 325 is machined from aluminum alloy 2A12, with 72 T-shaped teeth, a pitch circle diameter of 84mm and a thickness of 10mm; the synchronous belt 324 adopts an XL series light-load belt with a bandwidth of 8mm; the active rotating end 326 and the driven rotating end 327 are both machined from aluminum alloy 2A12, with an outer diameter of 120mm and a thickness of 10mm, one end of which is provided with a slide groove matching the transmission blocks on the submagnetic experimental component 1 and the control component 2, with a groove width of 10mm, and the other end is provided with a straight shaft with a diameter of 8mm and a length of 35mm. The motor 321 is fixed in the movable seat 322 with two M3 set screws. After the driving pulley 3223 is installed on the motor shaft, the movable seat 322 is installed on the lower part of one side of the active support frame 313 and fixed with four M3 screws. After the shaft of the active rotating end 326 passes through the bearing hole on the upper part of the active support frame 313, the driven pulley 325 is installed on it and bonded with 502 to prevent slipping. The synchronous belt 324 is installed between the driving pulley 323 and the driven pulley 325. The driven rotating end 327 passes through the inner hole of the driven support frame 314 bearing through the end shaft on it and is installed on the driven support frame 314, and is also bonded with 502 to prevent slipping.

[0060] The sample tray 33 is formed by stamping a 1 mm thick stainless steel plate, and the bottom of the tray is evenly distributed with small holes with a diameter of 10 mm.

[0061] The main body structure 41, upper cover 42 and rear cover 43 of the shell 4 are all formed by 3D printing with photosensitive resin, with a wall thickness of 3 mm. The upper cover 42 and rear cover 43 are fixed to the main body structure 41 by M3 screws; the insulation layer 45 is integrally foamed by polyurethane foam material, and the insulation layer 45 is installed inside the main body structure 41.

[0062] The CO2 gas cylinder in the cell culture environment control component 6 is an aluminum flat-bottom 2L pressure bottle, the pressure reducing valve is made of bipolar stainless steel, the solenoid valve is a 12V normally closed gas solenoid valve, the CO2 concentration sensor is an infrared detection sensor with a measurement range of 0-10%, and the pressure tube uses a 3mm outer diameter polytetrafluoroethylene material hard gas tube with an inner diameter of 1mm. The solenoid valve and the CO2 concentration sensor are fixed to the base plate by 2 M2 screws.

[0063] The heater in the temperature control component adopts a polyimide film heater with a heating voltage of 24V, a heating resistance of 24Ω, a size of 40mm◇40mm, and is directly attached to the lower part of the driven support frame 314 through adhesive. The 12V convection fan is installed in front of the polyimide film heater and fixed with 4 M3 screws. The temperature sensor adopts a standard digital sensor module and is installed in the middle position of the base plate 311 through 4 M2 screws.

[0064] The working mode is as follows:

[0065] Two sub-magnetic experimental components 1 and two control components 2 are installed on the transmission component 32. Three sample racks are installed in each sub-magnetic experimental component 1 and control component 2 to accommodate 6 T25 cell culture bottles. A total of 24 T25 samples are installed on the four components. Multiple spare T25 cell samples can be placed on the sample tray 33 as spare experimental samples. Close the upper cover 42 to form a closed experimental area in the device. Enter the experimental parameters as required on the display screen, and the control system 5 will control one sub-magnetic experimental component 1 and one control component 2 to rotate according to the set parameters, and the other one sub-magnetic experimental component 1 and one control component 2 remain stationary, so that four experimental samples in the normal experimental group, low gravity experimental group, sub-magnetic experimental group, and low gravity and sub-magnetic composite experimental group can be established synchronously, as well as spare samples cultured in the same environment. At the same time, in the control system 5, the temperature of the cell experiment area is controlled within the range of 37℃±0.5℃, and the CO2 concentration is controlled within the range of 5%±0.3%, forming environmental conditions that meet the cell growth in the cell experiment area and ensuring the long-term development of related experiments. During the experiment, the experimental status is displayed in real time on the display screen.

[0066] The system has the following technical effects:

[0067] 1) Ability to establish low-gravity experimental environments at different levels;

[0068] 2) Ability to establish a uniform hypomagnetic environment with different magnetic field strengths that meets the requirements of cytological experiments;

[0069] 3) Ability to establish a low gravity and hypomagnetic combined cytological experimental environment;

[0070] 4) Ability to simultaneously establish five types of experimental samples: normal experimental group, normal control group, low gravity experimental group, hypomagnetic experimental group, and low gravity and hypomagnetic combined experimental group;

[0071] 5) It can autonomously regulate the environmental parameters of the cell experimental area during the experiment to meet the requirements of cell growth and realize the long-term development of related experiments.

[0072] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. It should be pointed out that a person skilled in the art can make several improvements and modifications without departing from the technical principles of the present invention, and these improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A cytological submagnetic effect experimental system, characterized in that: It comprises a hypomagnetic experimental component and a control component. The hypomagnetic experimental component is used to establish a hypomagnetic environment with a low magnetic field strength that meets the experimental requirements in the area where the cell sample is located, and comprises an end cover, an experimental component sample rack, a cylindrical tubular coil, a magnetic isolation component, a convection fan and a slip ring; the slip ring and the convection fan are fixed to one end of the magnetic isolation component; the cylindrical tubular coil is installed inside the magnetic isolation component; the experimental component sample rack is installed inside the cylindrical tubular coil through a track inside the cylindrical tubular coil; the end cover is buckled on the other end of the magnetic isolation component; The control assembly is used to establish an experimental environment for the control sample group of the experiment, and includes a cylinder cover, a control assembly sample rack, a track, a transmission block, and a cylinder; the track is bonded inside the cylinder, the transmission block is fixed at both ends of the outer surface of the cylinder, the control assembly sample rack is installed on the track, and the cylinder cover is buckled at the open end of the cylinder; The end cover is composed of an inner cover, a magnetic isolation plate and an outer cover, and the inner cover and the outer cover are respectively bonded to two sides of the magnetic isolation plate; The experimental component sample rack is used to install the experimental sample, and includes a main structure and a buffer, and the buffer is bonded to the main structure; The cylindrical tubular coil is used to generate a uniform magnetic field required by the experiment, and includes a track, a support tube, and an enameled wire spirally wound outside the support tube, wherein the track is bonded to the inner surface of the support tube; The magnetic isolation component is used to isolate the magnetic field in the experimental environment, and includes a magnetic isolation layer, a protective outer layer, a protective inner layer and a transmission block. The magnetic isolation layer is sleeved outside the protective inner layer, the protective outer layer is sleeved outside the magnetic isolation layer, and the transmission block is fixedly connected to both ends of the protective outer layer. The cytological hypermagnetic effect experimental system also includes a shell, which is a box structure, and the hypermagnetic experimental components and the control components are arranged in the shell to form a closed cell experimental area in the shell; The housing is composed of a main body structure, an upper cover, a rear cover, a hinge and a heat-insulating layer. The main body structure is connected to the upper cover by the hinge, the main body structure is connected to the rear cover by screws, and the heat-insulating layer is installed inside the main body structure. The cytological submagnetic effect experimental system also includes a cell culture environment control component and a control system; the cell culture environment control component is used to establish the temperature and CO2 concentration conditions required by the cell experiment in the cell experiment area; the control system is used to regulate the cell culture environment control component to work according to the experimental requirements; the cell culture environment control component includes a temperature control component and a CO2 concentration control component, the CO2 concentration control component includes a CO2 gas cylinder, a pressure reducing valve, a solenoid valve, a CO2 concentration sensor and a pressure pipe, the pressure reducing valve is installed on the CO2 gas cylinder, the pressure reducing valve and the solenoid valve are connected through the pressure pipe, and the CO2 concentration sensor is arranged in the cell experiment area in the shell; the temperature control component is used to adjust the temperature of the cell experiment area, and includes a heater, a convection fan and a temperature sensor, and the temperature sensor is arranged in the cell experiment area in the shell; The control system includes a control circuit board and a display screen, wherein the control circuit board is installed on the rear cover of the housing, and the display screen is installed on the upper cover of the housing, and the control circuit board is electrically connected to the cell culture environment control component and the display screen; The cytological hypomagnetic effect experimental system integrates a low-gravity experimental component for simultaneously realizing low-gravity and hypomagnetic composite environment simulation and automatic control of the cell culture environment; the low-gravity experimental component includes a basic component, a transmission component and a sample tray; the transmission component is installed in the basic component, and the sample tray is installed on the upper part of the transmission component; the basic component is used to support the transmission component and the sample tray in the low-gravity experimental component; the transmission component is used to drive the hypomagnetic experimental component and the control component to rotate according to experimental requirements; the basic component includes a bottom plate, a side plate, an active support frame, a driven support frame, and a bearing, and the basic component is composed of a bottom plate, a side plate, an active support frame, and a driven support frame to form a rectangular box structure with an open upper end, the bottom plate is arranged on the bottom surface of the rectangular box structure, the active support frame and the driven support frame are arranged on one group of opposite sides of the rectangular box, and the two side plates are arranged on another group of opposite sides of the rectangular box. The support frame is provided with a plurality of bearing mounting holes, and the bearings are mounted in the bearing mounting holes on the active support frame and the driven support frame; the transmission component comprises a motor, a movable seat, a driving pulley, a synchronous belt, a driven pulley, an active rotating end and a driven rotating end, the motor output end is fixed to the movable seat by screws, the driving pulley is mounted on the motor output shaft, the movable seat with the motor and the driving pulley is fixed to the active support frame by screws, the active rotating end passes through the inner hole of the active support frame bearing through its end shaft and is mounted on the active support frame, the driving pulley is mounted on the end shaft of the active rotating end, the driven rotating end passes through the inner hole of the driven support frame bearing through its end shaft and is mounted on the driven support frame, the synchronous belt is mounted between the driving pulley and the driven pulley, and the end faces of the active rotating end and the driven rotating end are provided with sliding grooves matching the transmission blocks on the sub-magnetic experimental component and the control component, so as to drive the sub-magnetic experimental component and the control component to rotate; The heater and convection fan in the cell culture environment control component are installed on the driven support frame, and the temperature sensor is installed in the middle position of the base plate of the basic component; the control circuit board in the control system is electrically connected to the gravity experiment low component.

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