A fully enclosed reaction liquid preparation device

Abstract

The present invention relates to a fully enclosed reaction liquid preparation device, including a reagent chamber shell, the reagent chamber shell is provided with a connecting through hole, a reagent chamber, a waste liquid chamber and a liquid exchanger installation position, the reagent chamber bottom is provided with a reagent chamber bottom hole, an upper cover is installed above the reagent chamber, a piston or an air bag is arranged in the liquid exchanger, and a communicating vessel is connected at the bottom. The present invention has a simple structure and occupies a small space; the working time is short: the reaction liquid configuration can be completed within 2 minutes, and the speed can be increased by 5-10 times compared with manual operation, and the fully automatic preparation is convenient to operate: sample dilution, volume quantification, reagent mixing, etc. are integrated in a cartridge, and various reaction liquid components do not need to be added manually, so that the whole process is fully automated.

Description

Technical Field

[0001] The invention belongs to the technical field of biological experiments and relates to a fully enclosed reaction liquid preparation device. Background Art

[0002] The preparation of nucleic acid detection reaction solution generally requires diluting the nucleic acid sample to the required concentration for detection, and then according to the detection requirements, a certain amount of nucleic acid sample, reaction buffer, enzyme solution, and primer solution are drawn and mixed evenly. Currently, nucleic acid detection has become a common detection method, but the preparation of nucleic acid detection reaction solution is usually mainly manual. The entire process not only requires professional technicians to complete, but also requires a separate liquid preparation laboratory to prevent cross contamination, which not only prolongs the time of nucleic acid detection but also limits the promotion and application of nucleic acid detection technology. Summary of the invention

[0003] The object of the present invention is to provide a fully enclosed reaction liquid preparation device that can solve the above-mentioned problems.

[0004] According to the technical solution provided by the present invention: a fully enclosed reaction liquid preparation device comprises a reagent chamber shell, the reagent chamber shell is provided with a connecting through hole, a reagent chamber, a waste liquid chamber and a liquid exchanger, the bottom of the reagent chamber is provided with a reagent chamber bottom hole, an upper cover is installed above the reagent chamber, a piston or an air bag is arranged in the liquid exchanger, and the lower part is connected to a communicating vessel; the slider is driven by a driving element, and the driving element comprises a mounting frame, a motor is vertically installed on the upper part of the mounting frame, a slider is slidably installed on the side of the mounting frame through a guide rail, the motor output shaft is connected to a screw rod, a nut seat is arranged outside the screw rod, one end of the nut seat is connected to the slider, and the other end is connected to the piston or the air bag through a transmission rod.

[0005] As a further improvement of the present invention, one or more sample addition holes are provided on the upper cover.

[0006] As a further improvement of the present invention, a connecting hole vertically penetrates the outer side of the liquid exchanger, and a connecting groove is opened on the connecting vessel. One end of the connecting groove is connected to the inner cavity of the liquid exchanger, and the other end is connected to the connecting hole.

[0007] As a further improvement of the present invention, the communicating hole and the bottom hole of the reagent chamber are on the same diameter.

[0008] As a further improvement of the present invention, the reagent chamber includes a sample chamber, a diluent chamber, a quantitative chamber, and a mixing chamber; the quantitative chamber is adjacent to the waste liquid chamber and can be connected to each other.

[0009] As a further improvement of the present invention, the sample chamber, the diluent chamber, the quantitative chamber, and the mixing chamber may be one or more.

[0010] As a further improvement of the present invention, a sample cavity bottom hole is opened at the bottom of the sample cavity, a diluent cavity bottom hole is opened at the bottom of the diluent cavity, a quantitative cavity bottom hole and a quantitative cavity liquid inlet channel are provided in the quantitative cavity, and a mixing cavity bottom hole is opened at the bottom of the mixing cavity.

[0011] As a further improvement of the present invention, the quantitative chamber bottom hole is located at the bottom of the quantitative chamber, the quantitative chamber liquid inlet channel liquid outlet is located at the upper part of the quantitative chamber, and the quantitative chamber liquid inlet channel liquid inlet is located at the bottom of the quantitative chamber.

[0012] As a further improvement of the present invention, the communicating vessel and the liquid exchanger are integrally connected.

[0013] As a further improvement of the present invention, the mixing chamber may be pre-loaded with a liquid reagent or a solid freeze-dried reagent.

[0014] The positive effects of this application are:

[0015] 1. The present invention has a simple structure and occupies little space; the working time is short: the reaction liquid can be prepared within 2 minutes, which is 5-10 times faster than manual operation.

[0016] 2. The present invention is fully automatic in preparation and easy to operate: sample dilution, volume quantification, reagent mixing, etc. are integrated in one cartridge, and there is no need to manually add various reaction solution components, thus achieving full automation of the entire process.

[0017] 3. The sealed card box of the present invention: only the sample needs to be added, and the rest of the operations are performed in the sealed card box, with multiple anti-pollution and anti-leakage reagents, reducing the risk of nucleic acid aerosol contamination, and no risk of leakage of test samples and reagents. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a structural schematic diagram of the present invention.

[0019] Figure 2 1 is a top view of the reagent chamber housing 100 of the present invention.

[0020] Figure 3 It is a top view of the upper cover 200 of the present invention.

[0021] Figure 4 It is a structural diagram of the coordination between the communicating vessel 300 and the base plate of the liquid exchanger 400 in the present invention. DETAILED DESCRIPTION

[0022] The specific implementation of the present invention will be further described below in conjunction with the accompanying drawings.

[0023] Figure 1-4It includes a reagent chamber shell 100, a sample chamber 101, a diluent chamber 102, a quantitative chamber liquid inlet channel 103, a quantitative chamber 104, a quantitative chamber bottom hole 105, a mixing chamber 106, an upper cover 200, a connecting vessel 300, a liquid exchanger 400, etc.

[0024] like Figure 1-4 As shown, the present invention is a fully enclosed reaction liquid preparation device, comprising a reagent chamber shell 100, an upper cover 200, a communicating vessel 300, and a liquid exchanger 400; the reagent chamber shell 100 has an instrument connection through hole 109 for connecting the reagent chamber shell 100 with an experimental instrument, a reagent chamber and a waste liquid chamber 110 are provided in the reagent chamber shell 100, an upper cover 200 is provided on the reagent chamber shell 100, a sample addition hole 201 is provided on the upper cover 200, and a sample addition hole 202 is provided in the reagent chamber shell 100. There is a liquid exchanger 400, in which a piston or an air bag is provided, and a connecting vessel 300 is provided below the liquid exchanger 400. A connecting groove 301 is opened on the connecting vessel 300. One end of the connecting groove 301 is connected to the inner cavity of the liquid exchanger 400, and the other end is connected to the connecting hole 401. The connecting hole 401 and the bottom hole of the reagent chamber are on the same diameter. The inner cavity of the liquid exchanger 400 and the bottom hole of the reagent chamber can be selectively connected by rotating the connecting vessel 300.

[0025] The reagent chamber at least includes a sample chamber 101, a diluent chamber 102, a quantitative chamber 104, and a mixing chamber 106. A reagent chamber bottom hole is provided at the bottom of each chamber, which can communicate with the inner cavity of the liquid exchanger 400 through a connecting vessel 300, and the position of the sample chamber 101 corresponds to the sample addition hole 201.

[0026] A connecting through hole 109 is provided inside the reagent chamber housing 100 for connecting with an experimental instrument.

[0027] The reagent chamber housing 100 has an inverted buckle, and the communicating vessel 300 is fastened by the inverted buckle of the chamber housing.

[0028] The quantitative chamber 104 and the waste liquid chamber 110 are adjacent to each other and can be communicated with each other.

[0029] The quantitative cavity 104 includes a quantitative cavity bottom hole 105 and a quantitative cavity liquid inlet channel 103. The quantitative cavity bottom hole 105 is located at the bottom of the quantitative cavity 104, the quantitative cavity liquid inlet channel 103 is arranged in the quantitative cavity 104, the liquid inlet of the quantitative cavity liquid inlet channel 103 is located at the bottom of the quantitative cavity 104, and the liquid outlet of the quantitative cavity liquid inlet channel 103 is located at the upper part of the quantitative cavity 104.

[0030] The communicating vessel 300 is freely rotatable.

[0031] The communicating vessel 300 and the liquid exchanger 400 may be integrally connected.

[0032] The diluent chamber 102 is pre-filled with diluent.

[0033] The mixing chamber 106 may be pre-loaded with liquid reagents or solid lyophilized reagents.

[0034] In the present invention, different reagents can be pre-installed in the sealed reagent chamber and no leakage can be achieved.

[0035] The reagent chamber is connected to the inner cavity of the liquid exchanger 400 through the communicating vessel 300. The liquid exchanger 400 can be connected to different reagent chambers by rotating the communicating vessel 300. The liquid can be transferred back and forth between the reagent chamber and the inner cavity of the liquid exchanger 400 by the up and down movement of the piston or the airbag.

[0036] The piston or airbag is driven by a driving element 500, which includes a mounting frame 501, a motor 502 is vertically mounted on the upper part of the mounting frame 501, a slider 502 is slidably mounted on the side of the mounting frame 501 through a guide rail, the output shaft of the motor 502 is connected to a screw rod 503, a nut seat 504 is arranged outside the screw rod 503, one end of the nut seat 504 is connected to the slider 502, and the other end is connected to the piston or airbag through a transmission rod.

[0037] The mounting frame 501 is installed on the top of the liquid exchanger 400 .

[0038] The motor 502 drives the nut seat 504 to move up and down through the screw rod 503, so as to achieve the rise or fall of the piston or the air bag.

[0039] The driving element may also be a micro cylinder or a micro oil cylinder.

[0040] 1. Sample dilution

[0041] The communicating vessel 300 is rotated to connect the bottom hole 111 of the sample chamber with the communicating vessel 300, and the piston or the airbag rises to transfer the sample to the liquid exchanger 400. The communicating vessel 300 is rotated to connect the bottom hole 112 of the diluent chamber with the communicating vessel 300, and the piston or the airbag descends to transfer the sample in the liquid exchanger 400 to the diluent chamber 102. Then the piston or the airbag rises and repeats up and down 5 times to fully mix the sample and the diluent.

[0042] 2. Volume Quantification

[0043] The communicating vessel 300 is rotated to connect the bottom hole 112 of the diluent chamber with the communicating vessel 300, the piston or the air bag rises, and the liquid in the diluent chamber 102 is transferred to the liquid exchanger 400, the communicating vessel 300 is rotated to connect the quantitative chamber liquid inlet channel 103 with the communicating vessel 300, the piston or the air bag descends, and the liquid in the liquid exchanger 400 is transferred from the upper part of the quantitative chamber 104 to the quantitative chamber 104, and the excess liquid overflows from the quantitative chamber 104 and flows into the waste liquid chamber 110.

[0044] 3. Transfer quantitative solution

[0045] The communicating vessel 300 is rotated to connect the bottom hole 105 of the quantitative chamber with the communicating vessel 300 , and the piston or the air bag rises to transfer the liquid in the quantitative chamber 104 to the liquid exchanger 400 .

[0046] 4. Dissolving lyophilized reagents

[0047] The communicating vessel 300 is rotated to connect the bottom hole 107 of the mixing chamber with the communicating vessel 300, the piston or the air bag descends, and the liquid in the liquid exchanger 400 is transferred to the reagent tube in the mixing chamber 106, and then the piston or the air bag rises and moves up and down 5 times to fully dissolve and mix the freeze-dried reagent.

[0048] 5. Post-treatment of reaction solution

[0049] The piston or the airbag rises to transfer the liquid in the reagent tube to the liquid exchanger 400, and the communicating vessel 300 is rotated to connect the sample chamber bottom hole 111 and the communicating vessel 300, and then the piston or the airbag descends to transfer the liquid in the liquid exchanger 400 to the sample chamber 101. The prepared reaction liquid in the sample chamber 101 can be directly taken out from the sample hole for subsequent processing.

[0050] In the present invention, other reserved cavities are activated when necessary.

[0051] In the present invention, different reagents can be pre-installed in the sealed reagent chamber and no leakage can be achieved.

[0052] In the present invention, the inner cavity of the liquid exchanger 400 is connected to each reagent cavity by rotating the communicating vessel 300, and the liquid is transferred back and forth between the reagent cavity and the inner cavity of the liquid exchanger 400 by the up and down movement of the piston or the airbag.

[0053] In the present invention, the reagent chamber housing 100 can be fixed and the reagent chamber can be connected by means of a module assembly (including a fixed chamber housing module, a piston or airbag driving assembly, and a communicating vessel driving assembly). The liquid is transferred between the reagent chamber and the liquid exchanger 400 by rotating the communicating vessel 300 and driving the piston or airbag up and down. The above functions can realize the fully enclosed automatic preparation function of the nucleic acid detection reaction solution.

[0054] In the present invention, subsequent processing of the reaction solution, such as heating, amplification and detection, etc., can be achieved by adding other structures or devices.

Claims

1. A fully enclosed automatic preparation method for nucleic acid detection reaction solution, characterized in that: The method uses a fully enclosed reaction liquid preparation device, which comprises a reagent chamber shell (100) and a driving element (500). The reagent chamber shell (100) is provided with a connecting through hole (109), a reagent chamber, a waste liquid chamber (110) and a liquid exchanger (400). The bottom of the reagent chamber is provided with a reagent chamber bottom hole. An upper cover (200) is installed above the reagent chamber. A piston or an air bag is arranged in the liquid exchanger (400), and the lower part is connected to a communicating vessel (300). The driving element (500) comprises a mounting frame (501). A motor (502) is vertically mounted on the upper part of the mounting frame (501); a slider is slidably mounted on the side of the mounting frame (501) via a guide rail; the slider is driven by a driving element (500); an output shaft of the motor (502) is connected to a screw rod (503); a nut seat (504) is disposed on the outer surface of the screw rod (503); one end of the nut seat (504) is connected to the slider; and the other end is connected to the piston or the airbag via a transmission rod; a connecting hole (401) is vertically penetrated through the outer side of the liquid exchanger (400); and a connecting vessel (300) is provided on the connecting vessel (300). A connecting groove (301) is provided, one end of the connecting groove is connected to the inner cavity of the liquid exchanger (400), and the other end is connected to the connecting hole (401); the connecting hole (401) and the bottom hole of the reagent chamber are on the same diameter; the reagent chamber comprises a sample chamber (101), a diluent chamber (102), a quantitative chamber (104), and a mixing chamber (106); the quantitative chamber (104) and the waste liquid chamber (110) are adjacent and connected to each other; a sample chamber bottom hole (111) is provided at the bottom of the sample chamber (101), and the diluent chamber (102) is provided at the bottom of the sample chamber (101). The bottom of the quantitative chamber (102) is provided with a diluent chamber bottom hole (112), the quantitative chamber (104) is provided with a quantitative chamber bottom hole (105) and a quantitative chamber liquid inlet channel (103), and the bottom of the mixing chamber (106) is provided with a mixing chamber bottom hole (107); the quantitative chamber bottom hole (105) is located at the bottom of the quantitative chamber (104), the liquid outlet of the quantitative chamber liquid inlet channel (103) is located at the upper part of the quantitative chamber (104), and the liquid inlet of the quantitative chamber liquid inlet channel (103) is located at the bottom of the quantitative chamber (104); the method comprises the following steps:

1. Sample dilution The communicating vessel (300) is rotated to connect the bottom hole (111) of the sample chamber with the communicating vessel (300), the piston or the air bag is raised to transfer the sample to the liquid exchanger (400), the communicating vessel (300) is rotated to connect the bottom hole (112) of the diluent chamber with the communicating vessel (300), the piston or the air bag is lowered to transfer the sample in the liquid exchanger (400) to the diluent chamber (102), and then the piston or the air bag is raised, and the up-and-down reciprocating motion is repeated (5 times) to fully mix the sample with the diluent; 2. Volume Quantification The communicating vessel (300) is rotated to connect the bottom hole (112) of the diluent chamber with the communicating vessel (300), the piston or the air bag is raised, and the liquid in the diluent chamber (102) is transferred to the liquid exchanger (400); the communicating vessel (300) is rotated to connect the quantitative chamber liquid inlet channel (103) with the communicating vessel (300), the piston or the air bag is lowered, and the liquid in the liquid exchanger (400) is transferred from the upper part of the quantitative chamber (104) to the quantitative chamber (104), and the excess liquid overflows from the quantitative chamber (104) and flows into the waste liquid chamber (110); 3. Transfer quantitative solution The communicating vessel (300) is rotated to connect the bottom hole (105) of the quantitative chamber with the communicating vessel (300), and the piston or the air bag rises to transfer the liquid in the quantitative chamber (104) to the liquid exchanger (400); 4. Dissolving lyophilized reagents The communicating vessel (300) is rotated to connect the bottom hole (107) of the mixing chamber with the communicating vessel (300), the piston or the air bag is lowered, the liquid in the liquid exchanger (400) is transferred to the reagent tube in the mixing chamber (106), and then the piston or the air bag is raised and reciprocated up and down (5) times to fully dissolve and mix the freeze-dried reagent; 5. Subsequent treatment of reaction solution The piston or the air bag rises to transfer the liquid in the reagent tube to the liquid exchanger (400), and the communicating vessel (300) is rotated to connect the bottom hole (111) of the sample chamber with the communicating vessel (300). Then, the piston or the air bag descends to transfer the liquid in the liquid exchanger (400) to the sample chamber (101); the prepared reaction liquid in the sample chamber (101) can be directly taken out from the sample hole for subsequent processing.

2. The fully enclosed automatic preparation method of nucleic acid detection reaction solution according to claim 1, characterized in that: The upper cover (200) is provided with one or more sample addition holes (201).

3. The fully enclosed automatic preparation method of nucleic acid detection reaction solution according to claim 1, characterized in that: There are one or more sample chambers (101), diluent chambers (102), quantitative chambers (104), and mixing chambers (106).

4. The fully enclosed automatic preparation method of nucleic acid detection reaction solution according to claim 1, characterized in that: The communicating vessel (300) and the liquid exchanger (400) are integrally connected.

5. The fully enclosed automatic preparation method of nucleic acid detection reaction solution according to claim 1, characterized in that: The mixing chamber (106) is pre-loaded with a solid freeze-dried reagent.

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