Test hole calibration device and calibration method for ultra-micro spectrophotometer

Abstract

The present invention relates to a test hole calibration device and calibration method for an ultra-micro spectrophotometer, wherein the ultra-micro spectrophotometer to be calibrated comprises an upper base and a lower base, an upper movable base is mounted on the upper base, a lower movable base is mounted on the lower base, an upper test hole is provided on the upper movable base, a lower test hole is provided on the lower movable base, and the diameters of the upper test hole and the lower test hole are equal and relatively arranged. The calibration device comprises a locating pin for inserting into the upper test hole and the lower test hole, and the diameter of the locating pin is equal to the diameter of the upper test hole. Aiming at the problem of misalignment of the upper and lower bases in the existing ultra-micro spectrophotometer, the present invention adopts a mechanical locating pin to calibrate the upper and lower bases, and takes the locating pin smooth and not stuck as effective calibration, so that the upper test hole and the lower test hole are concentric, which is convenient for alignment when installing the optical fiber, and the coaxial deviation value is within the range of 0.06mm, thereby improving the stability and accuracy of the spectrophotometer.

Description

Technical Field

[0001] The invention relates to the field of spectrophotometer calibration, and in particular to a test hole calibration device and a calibration method for an ultra-micro spectrophotometer. Background Art

[0002] The ultra-micro spectrophotometer is an instrument that integrates nucleic acid detection, protein detection, cell solution detection, and full-wavelength spectrum detection. It needs to detect nucleic acids, proteins, and cell solutions quickly and accurately, so it must have a precise structure to make this compact instrument popular and recognized in any field, whether in biological laboratories or research institutes. This instrument is easy to use, consumes less sample (only 0.5-2μl), does not require preheating, can quickly clean up residual samples, does not require cuvettes or other sample positioning devices, and does not require sample dilution.

[0003] The accuracy of the test results of the ultra-micro spectrophotometer depends on whether the test hole base is aligned. If the test base is not aligned, the amount of light passing through the sample solution each time will be inconsistent, and the amount of light absorbed will be inconsistent, resulting in extremely poor stability of each measurement. Because the absorbance test depends on the amount of light passing through the liquid column, the instability of the light passing through the liquid column will ultimately cause inaccurate test results, large data deviations, and large coefficients of variation, which will directly affect the researcher's subsequent downstream experiments. Summary of the invention

[0004] The technical problem to be solved by the present invention is the problem of inaccurate base alignment in existing ultra-micro spectrophotometers.

[0005] In order to solve the above technical problems, the present invention first proposes a test hole calibration device for an ultra-micro spectrophotometer, wherein the ultra-micro spectrophotometer to be calibrated includes an upper base and a lower base, the upper base is installed with an upper movable seat, the lower base is installed with a lower movable seat, the upper movable seat is provided with an upper test hole, the lower movable seat is provided with a lower test hole, the upper test hole and the lower test hole have equal diameters and are arranged relatively, and the calibration device includes positioning pins for inserting into the upper test hole and the lower test hole, and the diameter of the positioning pin is equal to the diameter of the upper test hole.

[0006] As a further improvement of the test hole calibration device of the ultra-micro spectrophotometer of the present invention, the upper movable seat includes a matching SMA upper fiber seat and an SMA upper nut, the SMA upper fiber seat is a capped bolt, the SMA upper fiber seat is provided with the upper test hole passing through its central axis, the screw of the SMA upper fiber seat is inserted upward into the upper base, and the cap of the SMA upper fiber seat abuts against the lower surface of the upper base. The lower movable seat includes a matching SMA lower fiber seat and an SMA lower nut, the SMA lower fiber seat is a capped bolt, the SMA lower fiber seat is provided with the lower test hole passing through its central axis, the screw of the SMA lower fiber seat is inserted downward into the lower base, and the cap of the SMA lower fiber seat abuts against the upper surface of the lower base. The calibration device also includes a positioning seat, which is a cylindrical structure, and its two end faces are respectively provided with grooves for accommodating the cap body of the SMA upper fiber seat and the cap body of the SMA lower fiber seat, and the radial dimensions of the grooves at both ends are respectively equal to the outer diameters of the cap body of the SMA upper fiber seat and the cap body of the SMA lower fiber seat, and a through-hole is also provided between the grooves at both ends, and the diameter of the connecting hole is ≥ the diameter of the upper test hole. Ultra-micro means that the spectrophotometer requires less test sample, only 0.5-2μL.

[0007] As a further improvement of the test hole calibration device of the ultra-micro spectrophotometer of the present invention, the outer shape of the positioning seat is cylindrical.

[0008] As a further improvement of the test hole calibration device of the ultra-micro spectrophotometer of the present invention, the diameter of the connecting hole is equal to the diameter of the positioning pin.

[0009] As a further improvement of the test hole calibration device of the ultra-micro spectrophotometer of the present invention, the groove depths at both ends of the positioning seat are respectively equal to the cap lengths of the SMA upper fiber seat and the SMA lower fiber seat.

[0010] As a further improvement of the test hole calibration device of the ultra-micro spectrophotometer of the present invention, the upper end of the positioning pin has a protruding ring with a diameter larger than the diameter of the upper test hole.

[0011] As a further improvement of the test hole calibration device of the ultra-micro spectrophotometer of the present invention, the upper test hole and the lower test hole are cylindrical holes, and the positioning pin is cylindrical.

[0012] As a further improvement of the test hole calibration device of the ultra-micro spectrophotometer of the present invention, the length of the positioning pin is greater than the sum of the lengths of the upper test hole and the lower test hole.

[0013] The present invention also provides a test hole calibration method for an ultra-micro spectrophotometer, which is applied to the test hole calibration device for the ultra-micro spectrophotometer. The calibration method comprises the following steps:

[0014] S1, insert the screw of the SMA upper fiber holder upward into the upper base, and screw the SMA upper nut halfway into the screw of the SMA upper fiber holder; insert the screw of the SMA lower fiber holder downward into the lower base, and screw the SMA lower nut halfway into the screw of the SMA lower fiber holder;

[0015] S2, lift the upper base of the spectrophotometer, so that the groove on the upper end surface of the positioning seat accommodates the cap body of the SMA upper fiber seat, and lower the upper base of the spectrophotometer so that the groove on the lower end surface of the positioning seat accommodates the cap body of the SMA lower fiber seat;

[0016] S3, inserting the positioning pin into the upper test hole, the connecting hole and the lower test hole from top to bottom, tightening the SMA upper nut and the SMA lower nut, and during the tightening process, fine-tuning the positions of the SMA upper fiber holder and the SMA lower fiber holder so that the positioning pin can be smoothly inserted and removed;

[0017] S4, take out the positioning pin, lift up the upper base of the spectrophotometer, and take out the positioning seat.

[0018] The spectrophotometer has two optical fiber lines, an upper optical fiber line, and an upper metal ring surrounding the central optical fiber is provided at the head of the upper optical fiber line. The upper metal ring is provided with an internal thread that matches the upper optical fiber seat of the SMA. The head of the lower optical fiber line is provided with a lower metal ring surrounding the central optical fiber. The lower metal ring is provided with an internal thread that matches the lower optical fiber seat of the SMA.

[0019] As a further improvement of the test hole calibration method of the ultra-micro spectrophotometer of the present invention, there is a step S5 after step S4: insert the central optical fiber of the upper optical fiber line of the spectrophotometer from top to bottom into the upper test hole of the SMA upper optical fiber seat, and tighten the upper metal ring on the screw of the SMA upper optical fiber seat; insert the central optical fiber of the lower optical fiber line of the spectrophotometer from bottom to top into the lower test hole of the SMA lower optical fiber seat, and tighten the lower metal ring on the screw of the SMA lower optical fiber seat to complete the alignment of the upper central optical fiber and the lower central optical fiber. The diameter of the upper central optical fiber = the diameter of the lower central optical fiber. After alignment, the spacing between the upper central optical fiber and the lower central optical fiber is 0.5mm. When performing a spectral test, the liquid to be tested is dripped onto the lower central optical fiber with a pipette, and the upper base of the spectrophotometer is covered. The gap between the upper central optical fiber and the lower central optical fiber forms a liquid column of the liquid to be tested, and the test can be started.

[0020] The present invention aims at the problems that the upper and lower bases of the existing ultra-micro spectrophotometer are not aligned, or the state in which the liquid column is not visible after the fully closed base is covered, or the liquid column formed by the automatic base is prone to bubbles, or the selected method is costly and difficult, etc., and the upper and lower bases are calibrated by mechanical positioning pins, which is convenient for alignment when installing the optical fiber, and the coaxial deviation value is within the range of 0.06mm. The calibration device and method of the present invention are simple and effective, convenient to wipe after dropping the sample, easy to observe, the liquid column is visible to the naked eye, the measurement data is stable, the reliability is strong, the accuracy is high, and the stability is good. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 The present invention is a cross-sectional schematic diagram of the test hole calibration device of the ultra-micro spectrophotometer in the upper base and the lower base.

[0022] Figure 2 A schematic diagram of the appearance of an ultra-micro spectrophotometer used in the calibration device of the present invention.

[0023] Figure 3 for Figure 1 A three-dimensional view of the positioning seat in FIG.

[0024] Figure 4 for Figure 1 A three-dimensional image of the SMA lower fiber holder.

[0025] Reference numerals: upper base 1 , lower base 2 , positioning pin 3 , SMA upper optical fiber seat 4 , SMA upper nut 5 , SMA lower optical fiber seat 6 , SMA lower nut 7 , positioning seat 8 , upper cover 11 , cross bar 21 . DETAILED DESCRIPTION

[0026] The embodiments of the present invention are described in detail below with reference to the accompanying drawings.

[0027] like Figure 1 and Figure 2 As shown, the present invention first proposes a test hole calibration device for an ultra-micro spectrophotometer, wherein the ultra-micro spectrophotometer to be calibrated includes an upper base 1 and a lower base 2, one end of the upper base 1 and one end of the lower base 2 are hinged by a rotating shaft, and the upper base 1 can rotate relative to the lower base 2. The upper base 1 is mounted with an upper movable seat, and the lower base 2 is mounted with a lower movable seat, the upper movable seat is provided with a cylindrical upper test hole, and the lower movable seat is provided with a cylindrical lower test hole, the upper test hole and the lower test hole have equal diameters and are arranged oppositely, and the calibration device includes a cylindrical positioning pin 3 for inserting into the upper test hole and the lower test hole, the diameter of the positioning pin 3 is equal to the diameter of the upper test hole, and the upper end of the positioning pin 3 has a protruding ring with a diameter greater than the diameter of the upper test hole.

[0028] The upper movable seat includes a matching SMA upper fiber seat 4 and an SMA upper nut 5. The SMA upper fiber seat 4 is a capped bolt. The SMA upper fiber seat 4 is provided with the upper test hole running through its central axis. The screw of the SMA upper fiber seat 4 is inserted upward into the upper base 1, and the cap of the SMA upper fiber seat 4 abuts against the lower surface of the upper base 1. The lower movable seat includes a matching SMA lower fiber seat 6 and an SMA lower nut 7. Figure 4 : is a three-dimensional diagram of the SMA lower fiber holder 6, the SMA lower fiber holder 6 is a capped bolt, the SMA lower fiber holder 6 is provided with the lower test hole penetrating its central axis, the screw of the SMA lower fiber holder 6 is inserted downward into the lower base, and the cap of the SMA lower fiber holder 6 abuts against the upper surface of the lower base 2. The SMA upper fiber holder 4 and the SMA lower fiber holder 6 are symmetrically arranged and have the same structure, and the SMA upper nut 5 and the SMA lower nut 7 are symmetrically arranged and have the same structure.

[0029] The calibration device also includes a positioning seat 8, such as Figure 3 As shown, the positioning seat 8 is a cylindrical structure, and grooves are respectively provided on the two end surfaces for accommodating the cap body of the SMA upper fiber seat 4 and the cap body of the SMA lower fiber seat 6. The radial dimensions of the grooves at both ends are respectively equal to the outer diameters of the cap body of the SMA upper fiber seat 4 and the cap body of the SMA lower fiber seat 6, and the depth of the grooves is respectively equal to the length of the cap body of the SMA upper fiber seat 4 and the cap body of the SMA lower fiber seat 6. A through-hole is also provided between the grooves at both ends, and the diameter of the through-hole = the diameter of the upper test hole = the diameter of the positioning pin.

[0030] The length of the positioning pin 3 is greater than the sum of the lengths of the upper test hole, the connecting hole and the lower test hole.

[0031] The present invention also provides a test hole calibration method for an ultra-micro spectrophotometer, which is applied to the test hole calibration device for the ultra-micro spectrophotometer. The calibration method comprises the following steps:

[0032] S1, the upper base 1 has an upper cover 11 (such as Figure 2 ), open the upper cover 11, insert the screw of the SMA upper fiber seat 4 upward into the upper base 1, and screw the SMA upper nut 5 halfway into the screw of the SMA upper fiber seat 4; insert the screw of the SMA lower fiber seat 6 downward into the lower base 2, and screw the SMA lower nut 7 halfway into the screw of the SMA lower fiber seat 6;

[0033] S2, lift the upper base 1 of the spectrophotometer, so that the groove on the upper end surface of the positioning seat 8 accommodates the cap body of the SMA upper fiber seat 4, and put down the upper base 1 of the spectrophotometer so that the groove on the lower end surface of the positioning seat 8 accommodates the cap body of the SMA lower fiber seat 6;

[0034] S3, insert the positioning pin 3 into the upper test hole, the connecting hole and the lower test hole from top to bottom, tighten the SMA upper nut 5 and the SMA lower nut 7, and during the tightening process, fine-tune the positions of the SMA upper fiber holder 4 and the SMA lower fiber holder 6 so that the positioning pin 3 can be smoothly inserted and removed;

[0035] S4, take out the positioning pin 3, lift up the upper base 1 of the spectrophotometer, take out the positioning seat 8, and close the upper cover 11.

[0036] The spectrophotometer has two optical fiber lines, upper and lower. The head of the upper optical fiber line is provided with an upper metal ring surrounding the central optical fiber, the outer surface of the central optical fiber is provided with a plastic outer skin, the upper metal ring is fixed on the plastic outer skin through a hard plastic part, and the upper metal ring is provided with an internal thread that matches the SMA upper optical fiber seat 4. The head of the lower optical fiber line is provided with a lower metal ring surrounding the central optical fiber, the outer surface of the central optical fiber is provided with a plastic outer skin, the lower metal ring is fixed on the plastic outer skin through a hard plastic part, and the lower metal ring is provided with an internal thread that matches the SMA lower optical fiber seat 6.

[0037] After step S4, there is step S5: insert the central optical fiber of the upper optical fiber line of the spectrophotometer from top to bottom into the upper test hole of the SMA upper optical fiber seat 4, and tighten the upper metal ring on the screw of the SMA upper optical fiber seat 4; insert the central optical fiber of the lower optical fiber line of the spectrophotometer from bottom to top into the lower test hole of the SMA lower optical fiber seat 6, and tighten the lower metal ring on the screw of the SMA lower optical fiber seat 6 to complete the alignment of the upper central optical fiber and the lower central optical fiber. The diameter of the upper central optical fiber = the diameter of the lower central optical fiber. After alignment, close the upper base 1 and the lower base 2. The spacing between the upper central optical fiber and the lower central optical fiber is 0.5mm, and the coaxial deviation value is within the range of 0.06mm. Among them, an abutment mechanism is provided between the upper base 1 and the lower base 2 to limit the distance between the upper center fiber and the lower center fiber. Specifically, a convex hemisphere is provided on the lower surface of the upper base 1, and a cross bar 21 is provided on the upper surface of the lower base 2. When the upper base 1 is closed, the convex hemisphere abuts on the cross bar 21, and the distance between the upper center fiber and the lower center fiber is limited to 0.5 mm. When performing a spectrum test, the liquid to be tested is dripped onto the lower center fiber with a pipette, and the upper base 1 of the spectrophotometer is covered. The gap between the upper center fiber and the lower center fiber forms a liquid column of the liquid to be tested, and the test can be started.

[0038] The present invention aims at the problems that the upper and lower bases of the existing ultra-micro spectrophotometer are not aligned, or the state in which the liquid column is not visible after the fully closed base is covered, or the liquid column formed by the automatic base is prone to bubbles, or the selected method is costly and difficult, etc., and a mechanical positioning pin is used to calibrate the upper and lower bases, and the positioning pin is smooth and not stuck as effective calibration, so that the SMA upper fiber seat 4 and the SMA lower fiber seat 6 are concentric (the upper test hole and the lower test hole are concentric), which is convenient for alignment when installing the optical fiber, and the coaxial deviation value is within the range of 0.06mm, thereby improving the stability and accuracy of the spectrophotometer. The calibration device and method of the present invention are simple and effective, convenient to wipe after dropping the sample, easy to observe, the liquid column is visible to the naked eye, the measurement data is stable, the reliability is strong, the accuracy is high, and the stability is good.

Claims

1. A test hole calibration device for an ultra-micro spectrophotometer, wherein the ultra-micro spectrophotometer to be calibrated comprises an upper base and a lower base, the upper base is mounted with an upper movable seat, the lower base is mounted with a lower movable seat, the upper movable seat is provided with an upper test hole, the lower movable seat is provided with a lower test hole, the upper test hole and the lower test hole have equal diameters and are arranged oppositely, characterized in that: The calibration device includes a positioning pin for inserting into the upper test hole and the lower test hole, and the diameter of the positioning pin is equal to the diameter of the upper test hole; the upper movable seat includes a matching SMA upper fiber seat and an SMA upper nut, the SMA upper fiber seat is a capped bolt, the SMA upper fiber seat is provided with the upper test hole passing through its central axis, the screw of the SMA upper fiber seat is inserted upward into the upper base, and the cap body of the SMA upper fiber seat abuts against the lower surface of the upper base; the lower movable seat includes a matching SMA lower fiber seat and an SMA lower nut, the SMA lower fiber seat is a capped bolt, the SMA lower fiber seat is provided with the lower test hole passing through its central axis, and the SMA lower The screw of the optical fiber seat is inserted downward into the lower base, and the cap body of the SMA lower optical fiber seat abuts against the upper surface of the lower base; the calibration device also includes a positioning seat, which is a cylindrical structure, and its two end surfaces are respectively provided with grooves for accommodating the cap body of the SMA upper optical fiber seat and the cap body of the SMA lower optical fiber seat, and the radial dimensions of the grooves at both ends are respectively equal to the outer diameters of the cap body of the SMA upper optical fiber seat and the cap body of the SMA lower optical fiber seat, and a connecting hole is also provided between the grooves at both ends, and the diameter of the connecting hole is ≥ the diameter of the upper test hole; the external shape of the positioning seat is cylindrical; the depth of the grooves at both ends of the positioning seat is respectively equal to the length of the cap body of the SMA upper optical fiber seat and the cap body of the SMA lower optical fiber seat.

2. The test hole calibration device of the ultra-micro spectrophotometer according to claim 1, characterized in that: The upper end of the positioning pin is provided with a protruding ring whose diameter is larger than the diameter of the upper testing hole.

3. The test hole calibration device of the ultra-micro spectrophotometer according to claim 1, characterized in that: The upper test hole and the lower test hole are cylindrical holes, and the positioning pin is cylindrical.

4. The test hole calibration device for an ultra-micro spectrophotometer according to claim 1, characterized in that: The length of the positioning pin is greater than the sum of the lengths of the upper test hole and the lower test hole.

5. A test hole calibration method for an ultra-micro spectrophotometer, characterized in that: Applicable to the test hole calibration device of the ultra-micro spectrophotometer according to claim 1, the calibration method comprises the following steps: S1, insert the screw of the SMA upper fiber holder upward into the upper base, and screw the SMA upper nut halfway into the screw of the SMA upper fiber holder; insert the screw of the SMA lower fiber holder downward into the lower base, and screw the SMA lower nut halfway into the screw of the SMA lower fiber holder; S2, lift the upper base of the spectrophotometer, so that the groove on the upper end surface of the positioning seat accommodates the cap body of the SMA upper fiber seat, and lower the upper base of the spectrophotometer so that the groove on the lower end surface of the positioning seat accommodates the cap body of the SMA lower fiber seat; S3, inserting the positioning pin into the upper test hole, the connecting hole and the lower test hole from top to bottom, tightening the SMA upper nut and the SMA lower nut, and during the tightening process, fine-tuning the positions of the SMA upper fiber holder and the SMA lower fiber holder so that the positioning pin can be smoothly inserted and removed; S4, take out the positioning pin, lift up the upper base of the spectrophotometer, and take out the positioning seat.

6. The test hole calibration method of the ultra-micro spectrophotometer according to claim 5, characterized in that: The spectrophotometer has two optical fiber lines, an upper optical fiber line and a lower optical fiber line. The head of the upper optical fiber line is provided with an upper metal ring surrounding the central optical fiber, and the upper metal ring is provided with an internal thread that matches the upper optical fiber seat of the SMA. The head of the lower optical fiber line is provided with a lower metal ring surrounding the central optical fiber, and the lower metal ring is provided with an internal thread that matches the lower optical fiber seat of the SMA. The diameter of the upper central optical fiber = the diameter of the lower central optical fiber; After step S4, there is a step S5: inserting the central optical fiber of the upper optical fiber line of the spectrophotometer into the upper test hole of the SMA upper optical fiber holder from top to bottom, and tightening the upper metal ring on the screw of the SMA upper optical fiber holder; inserting the central optical fiber of the lower optical fiber line of the spectrophotometer into the lower test hole of the SMA lower optical fiber holder from bottom to top, and tightening the lower metal ring on the screw of the SMA lower optical fiber holder, so as to complete the alignment of the upper central optical fiber and the lower central optical fiber.

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