A device for collecting corn root bleeding fluid

Abstract

A device for collecting corn root bleeding sap, which relates to a device for collecting root bleeding sap of crops. It solves the problem that the absorbent cotton becomes saturated and the bleeding sap overflows when the bleeding sap is collected in the prior art, resulting in inaccurate research results. The timing device and the linkage device of the present invention are connected by a lifting rod, and the linkage device is assembled with the lifting rod. The upper end of the lifting rod is fixed at the center of the circle of the upper cover plate of the cover body, and the lower end of the lifting rod is connected to the center of the circle of the upper surface of the extruder. The absorbent cotton is arranged below the extruder, and an adjustable fixing ring is provided below the absorbent cotton. An annular liquid collector is provided on the outer side of the adjustable fixing ring, and a liquid collecting bottle is provided below the right side of the annular liquid collector. The present invention blocks foreign objects such as wind, rain, and insects from entering the cover body, ensures the cleanliness of the cover body during sampling, saves labor, and can arbitrarily set the sampling time interval.

Description

Technical Field

[0001] The invention relates to a device for collecting crop root bleeding sap, in particular to a device for collecting corn root bleeding sap. Background Art

[0002] The root system is one of the three major organs of corn. It has the function of absorbing water and nutrients from the soil, and also has the function of synthesizing amino acids and growth regulators. Some of these substances absorbed and synthesized by the root system participate in the synthesis of root assimilates and various physiological activities, and are transported to the aboveground part together with the xylem sap, affecting the growth and yield formation of corn. Researchers have realized that understanding the changes in the composition of xylem sap in living plants is the first basic condition for understanding the root-crown relationship. It is now recognized that xylem sap and its components and changes in the concentration of each component are affected by many factors, such as the physiological activity of the root system and the aboveground part, the aeration of the land, the changes in soil moisture, temperature and other conditions will be reflected in the xylem. Researchers have paid extensive attention to corn xylem sap, because by studying the flow intensity of xylem sap transported from the root system to the aboveground part, and the composition of xylem sap, the absorption and synthesis functions of the root system can be explored. Therefore, the study of the quantity and composition of xylem sap is an important part of the study of corn root physiology. Whether the sap collected when studying the composition of xylem sap can reflect the changes in sap in corn under natural conditions has become a technical problem faced by researchers. At present, the method of collecting root bleeding sap is usually adopted. Root bleeding sap connects the aboveground part and the root, and transports the root absorption and synthesis substances to the aboveground part. Root bleeding sap is an effective indicator for evaluating the overall physiological activity of the root system without actually digging the root system. This method is a natural method of collecting bleeding sap by relying on corn root pressure. When it is necessary to collect bleeding sap from corn after jointing, all the aboveground parts of the corn are removed with a sharp and clean knife at the base of the corn stalk, and the aboveground part is discarded. The blade of the corn stalk at the base is horizontal and not tilted. Then, put the prepared absorbent cotton on the blade, and then put a plastic bag on the outside of the absorbent cotton, and put them on the base of the stem node with a rubber band. The root bleeding sap continues to flow out from the wound and is absorbed by the absorbent cotton. When the absorbent cotton is saturated, remove the rubber band and plastic bag, squeeze out the liquid in the absorbent cotton, and put the absorbent cotton on the blade of the stem node again until the collection time is over. When collecting bleeding fluid, the cotton wool often becomes saturated, the bleeding fluid overflows, and is lost after flowing out, which leads to inaccurate research results. In addition, due to the strong absorption capacity of the corn root system, it is often necessary to manually remove the cotton wool midway, squeeze out the liquid inside, and then re-place it on the incision to continue collecting the bleeding fluid. This requires researchers to repeat the operation regularly, which is time-consuming and laborious. In addition, if it rains during the collection process, rain splashes soil onto the surface of the plastic bag, or rain falls on the surface of the plastic bag. In the process of removing the plastic bag, it is very easy for rain or rain with soil to contact with the cotton wool, thus affecting the accuracy of the experimental results. Summary of the invention

[0003] The purpose of the present invention is to solve the problem that when collecting corn root bleeding sap, the absorbent cotton is often saturated and the bleeding sap overflows and is lost, thereby causing inaccurate research results. A device for collecting corn root bleeding sap is provided. The specific technical solution to solve the problem is as follows:

[0004] The invention discloses a device for collecting corn root bleeding fluid, which comprises a cover body, an upper cover plate of the cover body, a timing device, a lifting rod connection line, a linkage device, a lifting rod, an extruder, absorbent cotton, an annular liquid collector, a liquid guide tube, a liquid collecting bottle and an adjustable fixing ring. The upper cover plate of the cover body is located at the upper end of the cover body, the timing device is fixed on the upper cover plate of the cover body, the timing device and the linkage device are connected by a lifting rod connection line, the linkage device and the lifting rod are assembled, the upper end of the lifting rod is fixed at the center of the circle of the upper cover plate of the cover body, the lower end of the lifting rod is connected to the center of the circle of the upper surface of the extruder, absorbent cotton is arranged below the extruder, the lower surface of the absorbent cotton is connected to the horizontal incision of the corn stem, an adjustable fixing ring is arranged below the absorbent cotton, an annular liquid collector is arranged on the outer side of the adjustable fixing ring, a liquid collecting bottle is arranged at the lower right side of the annular liquid collector, and the annular liquid collector and the liquid collecting bottle are connected by the liquid guide tube.

[0005] The advantages of the device for collecting corn root bleeding sap of the present invention are: 1. The device uses a cover to isolate the corn stems to be sampled from the outside world, blocking foreign objects such as wind, rain, and insects from entering the cover, ensuring the cleanliness of the internal space of the cover during the sampling time; 2. A timer is used to control the operation of the lifting rod and the squeezer, so that the squeezer can pressurize the liquid in the absorbent cotton at a regular time, eliminating the manual timed squeezing of the absorbent cotton, thereby saving labor; 3. The device is not restricted by time, and the sampling time interval can be set arbitrarily. In the past, manual operations were used to sample the root bleeding sap, mostly from the morning to the next 12 hours. Sampling is mostly carried out during the day, which brings limitations to the research work. However, this device can collect corn root bleeding sap 24 hours a day for analysis and research. BRIEF DESCRIPTION OF THE DRAWINGS

[0006] Figure 1 is a schematic diagram of the structure of the device of the present invention, Figure 2 yes Figure 1 Schematic diagram of the structure of the absorbent cotton, adjustable fixing ring, annular liquid collector and liquid collecting bottle. DETAILED DESCRIPTION

[0007] Specific implementation method 1. Combination Figure 1 , Figure 2Describe this embodiment, this embodiment is composed of a cover body 1, a cover body upper cover plate 2, a timing device 3, a lifting rod connection line 4, a linkage device 5, a lifting rod 6, a squeezer 7, an absorbent cotton 8, an annular liquid collector 9, a liquid guide tube 10, a liquid collection bottle 11 and an adjustable fixing ring 12. The cover body upper cover plate 2 is located at the upper end of the cover body 1, the timing device 3 is fixed on the cover body upper cover plate 2, the timing device 3 and the linkage device 5 are connected by the lifting rod connection line 4, the linkage device 5 is equipped with the lifting rod 6, and the lifting rod 6 is fixed at the center of the upper cover plate 2 of the cover body, the lower end of the lifting rod 6 is connected to the center of the upper surface of the squeezer 7, the absorbent cotton 8 is arranged below the squeezer 7, the lower surface of the absorbent cotton 8 is connected to the horizontal incision of the corn stem, an adjustable fixing ring 12 is provided below the absorbent cotton 8, an annular liquid collector 9 is provided on the outside of the adjustable fixing ring 12 of the corn stem, a liquid collecting bottle 11 is provided at the lower right side of the annular liquid collector 9, and the annular liquid collector 9 and the liquid collecting bottle 11 are connected by a liquid guide tube 10.

[0008] Specific implementation method 2: Combination Figure 1 , Figure 2 Describing this embodiment, the annular liquid collector 9 and the adjustable fixing ring 12 described in this embodiment are both elliptical.

[0009] Specific implementation method 3: Combination Figure 1 , Figure 2 Describing this embodiment, the lower part of the annular liquid collector 9 described in this embodiment is elliptical.

[0010] Specific implementation method 4. Combination Figure 1 , Figure 2 Describing this embodiment, the centers of the annular liquid collector 9 and the adjustable fixing ring 12 described in this embodiment are coaxial with the lifting rod 6 .

[0011] Specific implementation method V. Combination Figure 1 , Figure 2 Describing this embodiment, the inner wall of the adjustable fixing ring 12 described in this embodiment is close to the outer diameter of the corn stem 13.

[0012] The steps of a method for collecting corn root bleeding fluid of the present invention are as follows:

[0013] 1. Preparation of corn stems: Use a sharp and clean knife to remove all the upper parts of the main stem of the corn to be tested, so that the horizontal incision on the surface of the corn stem 13 is elliptical, and its major diameter and minor diameter are d1 and d2 respectively, and the height from the corn stem incision to the ground is h1 (the height of the corn stem is determined according to the requirements and the actual growth of the corn plant);

[0014] Second, clean the side wall below the corn stem 13, and put the adjustable fixing ring 12 on the outer wall of the upper part of the corn stem 13 that has been cleaned in advance;

[0015] 3. The absorbent cotton 8 is made into an ellipse, the shape of the ellipse is similar to the cut of the corn stem 13, the major diameter of the absorbent cotton 8 is D1 and the minor diameter is D2, d1+h2≥D1≥d1, d2+h2≥D2>d2, the thickness of the absorbent cotton 8 is h3, and the range of h3 is 0.4-1.5 cm;

[0016] Fourth, the extrusion time of the timing device 3 of the device is set, and the extruder 7 extrudes once every 1.5 to 2.5 hours;

[0017] 5. Place the cover 1 so that the corn stalk 13 is located at the center of the cover 1, and the lower part of the cover 1 is in contact with the ground soil. The maximum extension distance of the lifting rod 6 is such that the squeezer 7 below the lifting rod 6 squeezes the absorbent cotton 8 according to the preset time. The maximum length of the lifting rod 6 Lmax = cover height H-corn stalk height h1-squeezer thickness h4;

[0018] Sixth, the lifting rod 6 moves up and down according to the interval time of the timing device 3, so that the squeezer 7 contacts with the absorbent cotton 8 and squeezes the absorbent cotton 8. As the lifting rod 6 descends, the lower surface of the squeezer 7 squeezes the liquid flowing out of the corn root system and absorbed by the absorbent cotton 8 into the annular liquid collector 9, and the liquid flows into the liquid collection bottle 11 along the liquid guide tube 10;

[0019] 7. When the collection is completed, the liquid collection bottle 11 is removed from the liquid guide tube 10 to complete the collection of the corn root bleeding sap.

Claims

1. A device for collecting corn root bleeding fluid, comprising a cover body, a cover upper cover plate, a timing device, a lifting rod connection line, a linkage device, a lifting rod, an extruder, absorbent cotton, an annular liquid collector, a liquid guide tube, a liquid collection bottle and an adjustable fixing ring, characterized in that: The upper cover plate of the cover body is located at the upper end of the cover body, the timing device is fixed on the upper cover plate of the cover body, the timing device and the linkage device are connected by a lifting rod, the linkage device is equipped with the lifting rod, the upper end of the lifting rod is fixed at the center of the upper cover plate of the cover body, the lower end of the lifting rod is connected to the center of the upper surface of the extruder, the absorbent cotton is arranged below the extruder, the lower surface of the absorbent cotton is connected to the horizontal incision of the corn stem, an adjustable fixing ring is provided below the absorbent cotton, the adjustable fixing ring is elliptical, the inner wall of the adjustable fixing ring is close to the outer diameter of the corn stem, an annular liquid collector is provided on the outside of the adjustable fixing ring, a liquid collecting bottle is provided at the lower right side of the annular liquid collector, the annular liquid collector and the liquid collecting bottle are connected by a liquid guide tube, and the lower part of the annular liquid collector cavity is elliptical.

2. The device for collecting corn root bleeding fluid according to claim 1, characterized in that: The centers of the annular liquid collector and the adjustable fixing ring are coaxial with the lifting rod.

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