A clinical-grade batch umbilical cord tissue inoculation device

Abstract

The present invention discloses a clinical-grade batch umbilical cord tissue inoculation device, which relates to the field of biotechnology and includes a connecting hose and a folding inoculation plate. The folding inoculation plate includes a first folding plate and a second folding plate hinged to the first folding plate, and a plurality of discharge pipes are arranged on the first folding plate and / or the second folding plate, and an inoculation hole is arranged at the end of any discharge pipe; the discharge end of the connecting hose is connected to the folding inoculation plate, and the head end of any discharge pipe is connected to the connecting hose; the feed end of the connecting hose is used to introduce umbilical cord tissue, and the umbilical cord tissue reaches the inoculation hole in turn through the connecting hose and the discharge pipe, and forms an inoculation tissue block in the inoculation hole. The present invention realizes clinical-grade, high-efficiency, batch inoculation tissue blocks, and the formed tissue blocks are flat, uniform in volume, neatly arranged, and standardized inoculation, and the umbilical cord tissue is not easy to lose moisture, the technology is simple, the operation is reliable, and the aseptic control of the production operation process is guaranteed, meeting the clinical mass production needs.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology, and relates to a primary culture and inoculation technology of umbilical cord tissue, and in particular to a clinical-grade batch umbilical cord tissue inoculation device for primary culture and inoculation of umbilical cord tissue. Background Art

[0002] At present, when umbilical cord tissue is inoculated, it is mostly inoculated by hand using glass rods, pipettes, plastic tubes, stainless steel tubes, etc. Pure manual inoculation is not only time-consuming and long, but also cannot achieve quantitative inoculation, low efficiency, cannot quantify the volume of each pile of tissue, and cannot achieve normalized and standardized primary tissue inoculation. Moreover, due to the large manual operation error, the umbilical cord block piles formed after inoculation are of different sizes and have no specifications, which makes it difficult to ensure the quantity and quality of primary cells. In addition, umbilical cord tissue inoculation is mostly carried out in a biological safety cabinet, and the airflow in the biological safety cabinet is easy to air-dry the tissue blocks for a long time, and the air-dried tissue cannot grow primary cells. Even if the degree of air-drying is not reached, manual inoculation is an open or semi-open operation, and the operation time is long, it is easy to introduce exogenous factors, causing pollution of primary cells, waste of resources, and causing certain economic losses. Summary of the invention

[0003] The purpose of the present invention is to provide a clinical-grade batch umbilical cord tissue inoculation device to solve the problem that the above-mentioned prior art cannot achieve quantitative inoculation of umbilical cord tissue, thereby resulting in low inoculation efficiency.

[0004] To achieve the above object, the present invention provides the following solutions:

[0005] The present invention provides a clinical-grade batch umbilical cord tissue inoculation device, which mainly comprises:

[0006] A folding inoculation plate, the folding inoculation plate comprising a first folding plate and a second folding plate hinged to the first folding plate, the first folding plate and / or the second folding plate being provided with a plurality of discharge pipes, and an inoculation hole being provided at the end of any of the discharge pipes;

[0007] A connecting hose, the discharge end of which is connected to the foldable inoculation plate, and the head end of any one of the discharge pipes is connected to the connecting hose; the feed end of the connecting hose is used to introduce umbilical cord tissue, and the umbilical cord tissue reaches the inoculation hole in turn through the connecting hose and the discharge pipe, and forms an inoculation tissue block in the inoculation hole.

[0008] Preferably, a funnel is further included, wherein the funnel is connected to the feeding end of the connecting hose and is used for introducing the umbilical cord tissue.

[0009] Preferably, it also includes a bracket that can be stably placed on the clean bench, and the funnel is placed on the bracket.

[0010] Preferably, a switch valve is provided at the bottom end of the funnel to control the opening and closing of the discharge port of the funnel.

[0011] Preferably, the feed end of the connecting hose is arranged at a higher height than the discharge end.

[0012] Preferably, the first folding plate and the second folding plate are hinged via a winding shaft; a sleeve is provided inside the winding shaft, one end of the sleeve is connected to the connecting hose, and the other end of the sleeve branches out into a plurality of the discharge pipes.

[0013] Preferably, the other end of the sleeve branches out into 8 pairs of the discharge pipes, and the two discharge pipes in any pair of the discharge pipes are respectively arranged on the first folding plate and the second folding plate, and the two discharge pipes in any pair of the discharge pipes are symmetrically distributed with the straight line around the tube axis as the center line.

[0014] Preferably, it also includes a folding control component, which includes a main rib, a supporting rib and a hinge seat; the main rib is vertically fixed at the hinge of the first folding plate and the second folding plate, and the hinge seat is slidably installed on the main rib; three supporting ribs are provided, and the three supporting ribs are distributed on the outer periphery of the main rib, and the top ends of the three supporting ribs are hinged on the hinge seat, and the bottom ends of the three supporting ribs are respectively slidably hinged with the first folding plate, the second folding plate and the hinge of the first folding plate and the second folding plate; the bottom end of any one of the supporting ribs is connected to a steel wire pull rod, and by pushing and pulling the steel wire pull rod, the bottom end of the corresponding supporting rib can be pushed and pulled, and the top end of the supporting rib is linked to drive the hinge seat to rise and fall along the main rib, thereby driving the remaining two supporting ribs to move synchronously, so as to achieve the purpose of regulating the folding angle between the first folding plate and the second folding plate.

[0015] Preferably, the bottom end of the umbrella supporting rib slidably hinged at the hinge of the first folding plate and the second folding plate is connected to the wire pull rod, and the wire pull rod can drive the umbrella supporting rib to slide along the hinge axis between the first folding plate and the second folding plate; the sliding trajectories of the bottom ends of the other two umbrella supporting ribs are perpendicular to the hinge axis between the first folding plate and the second folding plate.

[0016] Preferably, the inoculation hole is a circular inoculation hole.

[0017] Compared with the prior art, the present invention has achieved the following technical effects:

[0018] The clinical-grade batch umbilical cord tissue inoculation device proposed by the present invention has a simple and reasonable structure. By arranging a plurality of discharge pipes on the folding inoculation plate, and configuring inoculation holes at the end of any discharge pipe, the umbilical cord tissue introduced through the connecting hose can be diffused to multiple discharge pipes at the same time, and inoculation tissue blocks are formed in the inoculation holes, which not only realizes one-time batch inoculation and improves the inoculation efficiency, but also makes the specifications of multiple inoculation tissue blocks formed at one time uniform due to the uniform specifications of each discharge pipe and each inoculation hole, and realizes quantitative precision. When in use, the folding inoculation plate is folded and placed in a culture bottle (culture dish), and then the folding inoculation plate is controlled to unfold at a certain angle, and the umbilical cord tissue is introduced and reaches the inoculation hole to form an inoculation tissue block, and then the folding inoculation plate is controlled to fold, so that the formed inoculation tissue block can fall into the culture bottle (culture dish); the whole operation process is relatively closed, the umbilical cord tissue is not easy to lose moisture, and the technology is simple, the operation is reliable, the operation is flexible, and the labor saving and fast operation not only ensures the aseptic control of the production operation process, but also can meet the clinical mass production needs, and has strong practicality. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0020] Figure 1 This is a schematic diagram of the overall structure of a clinical-grade batch umbilical cord tissue inoculation device disclosed in an embodiment of the present invention;

[0021] Figure 2 It is a schematic structural diagram of a folding inoculation plate disclosed in an embodiment of the present invention;

[0022] Figure 3 It is a schematic diagram of the installation of the folding control assembly disclosed in the embodiment of the present invention;

[0023] Figure 4 It is a structural schematic diagram of a folding control component disclosed in an embodiment of the present invention;

[0024] Among them, the accompanying drawings are marked as: 001, clinical-grade batch umbilical cord tissue inoculation device; 1, bracket; 2, switch valve; 3, connecting hose; 4, funnel; 5, folding inoculation plate; 51, first folding plate; 52, second folding plate; 6, inoculation hole; 7, sleeve; 8, winding axis; 9, discharge pipe; 10, main rib; 11, supporting rib; 12, fixing clamp; 13, wire pull rod; 14, hinged seat; 15, first slide groove; 16, second slide groove; 17, slider; 18, pull ring; 19, limit block; 20, funnel cover. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] The purpose of the present invention is to provide a clinical-grade batch umbilical cord tissue inoculation device to solve the problem that the prior art cannot achieve quantitative inoculation of umbilical cord tissue, resulting in low inoculation efficiency.

[0027] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0028] Embodiment 1:

[0029] like Figure 1-3 As shown, this embodiment provides a clinical-grade batch umbilical cord tissue inoculation device 001, which mainly includes five parts: a bracket 1, a connecting hose 3, a funnel 4, a folding inoculation plate 5, and a folding control assembly. The folding inoculation plate 5 includes a first folding plate 51 and a second folding plate 52 hinged to the first folding plate 51. The first folding plate 51 and the second folding plate 52 are both provided with a plurality of discharge pipes 9, and an inoculation hole 6 is provided at the end of any discharge pipe 9. The funnel 4 can be stably placed on the bracket 1, and a switch valve 2 is provided at its bottom end, which is connected to the folding inoculation plate 5 through a connecting hose 3. One end of the connecting hose 3 is connected to the funnel 4, and the other end is connected to the folding inoculation plate 5. A tube axis 8 (hinge axis) is arranged at the folded part of the first folding plate 51 and the second folding plate 52. Both the first folding plate 51 and the second folding plate 52 can be turned over around the tube axis 8. A sleeve 7 is arranged inside the tube axis 8. One end of the sleeve 7 is connected to the connecting hose 3, and the other end branches out 8 pairs of discharge pipes 9. Among them, two discharge pipes 9 in any pair of discharge pipes 9 are arranged on the first folding plate 51 and the second folding plate 52 respectively, and the two discharge pipes 9 in any pair of discharge pipes 9 are symmetrically distributed with the straight line where the tube axis 8 is located as the center line, such as Figure 2 Each discharge pipe 9 is provided with an inoculation hole 6 at its end, so that the umbilical cord tissue can be inoculated into a culture bottle (or culture dish) in 8×2 (rows).

[0030] In this embodiment, Figure 1 As shown, the funnel 4 is provided with a funnel cover 20 to isolate the tissue in the funnel 4 from contact with the outside world.

[0031] In this embodiment, Figure 3As shown, a folding control assembly for controlling the folding of the folding inoculation board 5 is installed on the folding inoculation board 5. The folding control assembly includes a main rib 10, three supporting ribs 11 and a hinge seat 14. The main rib 10 is vertically fixed at the hinge of the first folding plate 51 and the second folding plate 52, that is, around the tube axis 8. The hinge seat 14 is slidably installed on the main rib 10; the three supporting ribs 11 are distributed on the periphery of the main rib 10, the top ends of the three supporting ribs 11 are all hinged on the hinge seat 14, and the bottom ends of the three supporting ribs 11 are respectively connected to the first folding plate 51, the second folding plate 52 and the hinge seat 14 around the tube axis. The upper top surface of the main umbrella rib 10 is hinged and slidable, and a first slide groove 15 is provided on the upper top surface of the winding tube shaft 8. The first slide groove 15 is located on one side of the main umbrella rib 10. A second slide groove 16 perpendicular to the first slide groove 15 is provided on the first folding plate 51 and the second folding plate 52 respectively. A slider 17 is installed in each of the first slide groove 15 and the two second slide grooves 16. The slider 17 can slide in the corresponding slide groove under the action of external force. The bottom ends of the three supporting umbrella ribs 11 are hinged on the three sliders 17 respectively. When in use, the folding inoculation plate 5 needs to be folded and loaded into a culture bottle (culture dish). Due to the small diameter of the culture bottle, it is inconvenient to directly control the folding of the folding inoculation plate 5. Therefore, the present embodiment also provides a wire pull rod 13. One end of the wire pull rod 13 is connected to the slider 17 located in the first slide groove 15, and the other end is connected to a pull ring 18 after being led out from the winding tube shaft 8. By pushing the wire pull rod 13 inward, the slider 17 in the first slide groove 15 can be pushed toward the bottom end of the main rib 10. At the same time, the top end of the supporting rib 11 connected to the slider 17 pushes the hinge seat 14 to slide upward along the main rib 10, thereby driving the top ends of the other two supporting ribs 11 to rise synchronously. The bottom ends of the other two supporting ribs 11 drive their respective sliders 17 along the second slide groove 16 to approach the bottom end of the main rib 10, thereby realizing the folding of the three supporting ribs 11 toward the main rib 10. When the three supporting ribs 11 are all attached to the outer periphery of the main rib 10, the first folding plate 51 and the second folding plate 52 are completely folded, and the angle between the two is substantially 0°. On the contrary, by pulling the wire rod 13 outward, the slider 17 in the first slide groove 15 can be pulled to slide toward the end away from the main rib 10. At the same time, the top of the supporting rib 11 connected to the slider 17 pulls the hinge seat 14 to slide downward along the main rib 10, thereby driving the tops of the other two supporting ribs 11 to descend synchronously, and the bottom ends of the other two supporting ribs 11 drive their respective sliders 17 to slide along the second slide groove 16 toward the end away from the main rib 10, thereby realizing that the three supporting ribs 11 are opened outward relative to the main rib 10 (similar to the opening principle of the umbrella frame). When the slider 17 at the bottom end of any supporting rib 11 can no longer slide, the opening angle of the three supporting ribs 11 reaches the maximum. Preferably, at this time, the first folding plate 51 and the second folding plate 52 are fully unfolded, and the angle between the two is or is approximately 180°. By controlling the wire rod 13 in the folding control assembly, the purpose of adjusting the folding angle between the first folding plate 51 and the second folding plate 52 can be achieved.

[0032] In this embodiment, Figure 3 As shown, a limiting block 19 is provided at the top of the main rib 10 to prevent the hinge seat 14 from slipping off the top of the main rib 10 .

[0033] In this embodiment, the first slide groove 15 is preferably arranged along the axial direction of the tube axis 8, and the two second slide grooves 16 are symmetrically arranged around the tube axis 8 as the center line, and the two second slide grooves 16 are arranged perpendicular to the first slide groove 15. The wire pull rod 13 can directly drive the umbrella supporting rib 11 to slide along the first slide groove 15, and the sliding tracks of the bottom ends of the other two umbrella supporting ribs 11 are perpendicular to the sliding track of the slider in the first slide groove 15.

[0034] In this embodiment, Figure 3 As shown, in order to ensure that the wire rod 13 keeps sliding horizontally when pushing and pulling the wire rod 13, a fixing clip 12 can be added between the bottom end of the main rib 10 and the end of the winding tube shaft 8. The fixing clip 12 is installed on the winding tube shaft 8 to fix the middle part of the wire rod 13 to prevent the middle part of the wire rod 13 from tilting during the pushing and pulling process, thereby affecting the folding of the folding inoculation plate 5.

[0035] In this embodiment, during actual operation, the feed end of the connecting hose 3 is higher than the discharge end to form a height difference between the feed end and the discharge end, so that the tissue fluid in the tube flows downward more easily, making it easier for the solution to flow into the folded inoculation plate 5.

[0036] In this embodiment, Figure 1 As shown, the bracket 1 can be stably placed in the clean bench.

[0037] In this embodiment, the foldable inoculation plate 5 is preferably made of stainless steel.

[0038] The specific method of using the clinical-grade batch umbilical cord tissue inoculation device 001 in this embodiment is as follows: a square bottom film with a length of 60 mm, a width of 60 mm, and a thickness of 1 mm is placed on the clean bench as a fixed base, and the clinical-grade batch umbilical cord tissue inoculation device 001 is placed on the fixed base; the funnel 4 can be stably placed in the bracket 1, and the funnel 4 is preferably a glass funnel with a volume of 20-30 ml; the length of the connecting hose 3 is 10-35 cm; the two sides of the folding inoculation plate 5 are symmetrical, and the first folding plate 51 and the second folding plate 52 are exactly the same in size, both of which are rectangular plate structures with a width of 10-30 mm and a length of 60-150 mm; the inoculation hole 6 is preferably 0.2-0.9 cm in area. 2inoculation circular hole; the through pipe between the sleeve 7 and the inoculation hole 6, that is, the length of the discharge pipe 9 is 3-10mm; the length of the main rib 10 and the supporting rib 11 is preferably 8mm; the length of the wire rod 13 is 60-70mm; the distance between two adjacent discharge pipes on the same folding plate is 2-15mm; the adjustable angle of the folding inoculation plate is 0-180 degrees.

[0039] When in use, first pour the umbilical cord tissue fluid into the funnel 4, open the switch valve 2, and let the umbilical cord tissue flow into the sleeve 7 of the folding inoculation plate 5 through the connecting hose 3. Tilt the folding inoculation plate at 45° and place it at 10 cm 2 In the culture dish, the umbilical cord tissue fluid is completely flowed into the sleeve 7, and the wire rod 13 is pulled to make the folded inoculation plate 5 flat in the culture dish, and flow into the inoculation hole 6 through the discharge pipe 9, so that the umbilical cord tissue is inoculated in the culture dish in 8×2 (rows), and the inoculation plate is folded by the wire rod 13 to seed the umbilical cord tissue into the culture dish in sequence. This embodiment realizes clinical efficient batch inoculation of tissue blocks, uniform flattening, and umbilical cord tissue moisture is not easy to lose, simple technology, reliable operation, flexible operation, labor-saving and fast, ensuring the aseptic control of the production operation process, and meeting the clinical mass production needs.

[0040] Since the folding angle between the first folding plate 51 and the second folding plate 52 in the folding inoculation plate 5 is adjustable, and the folding angles between the first folding plate 51 and the second folding plate 52 are different, the spacing between the inoculated tissue block arrangement rows (8 tissue blocks per row) formed on the first folding plate 51 and the inoculated tissue block arrangement rows (8 tissue blocks per row) formed on the second folding plate 52 are different. Therefore, the column spacing when the umbilical cord tissue blocks are implanted in the culture dish can be adjusted by adjusting the folding angle between the first folding plate 51 and the second folding plate 52.

[0041] It can be seen that the clinical-grade batch umbilical cord tissue inoculation device of this embodiment can complete the tissue block inoculation work in a very short time, and the inoculation is carried out in a relatively closed environment, which greatly reduces the risk of contamination by exogenous factors. In addition, the inoculation device can achieve quantitative inoculation, uniform volume of each pile of tissue, simple and controllable operation, and has good normalization and standardization, which is convenient for establishing standardized operating procedures for primary cell preparation, can significantly reduce batch differences, and provide favorable guarantees for the industrial preparation of umbilical cord mesenchymal stem cells.

[0042] The clinical-grade batch umbilical cord tissue inoculation device disclosed in the present invention is also suitable for the preparation of primary cells from tissue samples such as placental tissue and skin tissue, and can be used for scientific research projects using the tissue block adherence method and clinical-grade primary cell expansion culture.

[0043] It should be noted that it is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention, and any figure mark in the claims should not be regarded as limiting the claims involved.

[0044] The present invention uses specific examples to illustrate the principles and implementation methods of the present invention. The above examples are only used to help understand the method and core ideas of the present invention. At the same time, for those skilled in the art, according to the ideas of the present invention, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A clinical-grade batch umbilical cord tissue inoculation device, characterized in that: The invention comprises a folding inoculation plate, a connecting hose and a folding control assembly, wherein: The folding inoculation plate comprises a first folding plate and a second folding plate hinged to the first folding plate, the first folding plate and / or the second folding plate are provided with a plurality of discharge pipes, and an inoculation hole is provided at the end of any of the discharge pipes; The discharge end of the connecting hose is connected to the folding inoculation plate, and the head end of any of the discharge pipes is connected to the connecting hose; the feed end of the connecting hose is used to introduce umbilical cord tissue, and the umbilical cord tissue reaches the inoculation hole in sequence through the connecting hose and the discharge pipe, and forms an inoculation tissue block in the inoculation hole; The first folding plate and the second folding plate are hinged by a winding axis; a sleeve is arranged in the winding axis, one end of the sleeve is connected to the connecting hose, and the other end of the sleeve branches out a plurality of the discharge pipes; the discharge pipes are arranged in pairs, and the two discharge pipes in any pair of the discharge pipes are respectively arranged on the first folding plate and the second folding plate, and the two discharge pipes in any pair of the discharge pipes are symmetrically distributed with the straight line where the winding axis is located as the center line; The folding control assembly comprises a main rib, a supporting rib and a hinge seat; the main rib is vertically fixed to the hinge of the first folding plate and the second folding plate, and the hinge seat is slidably installed on the main rib; three supporting ribs are provided, and the three supporting ribs are distributed on the periphery of the main rib, and the top ends of the three supporting ribs are hinged to the hinge seat, and the bottom ends of the three supporting ribs are respectively slidably hinged to the first folding plate, the second folding plate and the hinge of the first folding plate and the second folding plate; any one of the supporting ribs is hinged to the hinge of the first folding plate and the second folding plate; The bottom end of the umbrella rib is connected to a wire pull rod, and the folding angle between the first folding plate and the second folding plate can be adjusted by pushing and pulling the wire pull rod, wherein the bottom end of the supporting umbrella rib slidably hinged at the hinge of the first folding plate and the second folding plate is connected to the wire pull rod, and the wire pull rod can drive the supporting umbrella rib to slide along the hinge axis between the first folding plate and the second folding plate; the sliding tracks of the bottom ends of the other two supporting umbrella ribs are perpendicular to the hinge axis between the first folding plate and the second folding plate.

2. The clinical-grade batch umbilical cord tissue inoculation device according to claim 1, characterized in that: A funnel is also included, which is connected to the feeding end of the connecting hose and is used for introducing the umbilical cord tissue.

3. The clinical-grade batch umbilical cord tissue inoculation device according to claim 2, characterized in that: It also includes a bracket that can be stably placed on a clean bench, and the funnel is placed on the bracket.

4. The clinical-grade batch umbilical cord tissue inoculation device according to claim 2, characterized in that: A switch valve is provided at the bottom end of the funnel to control the opening and closing of the discharge port of the funnel.

5. The clinical-grade batch umbilical cord tissue inoculation device according to claim 1, characterized in that: The feed end of the connecting hose is arranged at a higher height than the discharge end.

6. The clinical-grade batch umbilical cord tissue inoculation device according to claim 1, characterized in that: The other end of the sleeve branches out into 8 pairs of discharge pipes.

7. The clinical-grade batch umbilical cord tissue inoculation device according to claim 1, characterized in that: The inoculation hole is a circular inoculation hole.

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