A temperature-adjustable biological cell engineering culture device and its use method

Abstract

The present invention provides a temperature-adjustable biological cell engineering culture device and a method of using the same, and relates to the field of cell engineering technology. The temperature-adjustable biological cell engineering culture device and a method of using the same include an incubator, a temperature control device, an air blowing device, a cover, a placement mechanism, a shock absorbing mechanism, and a support plate. The top of the incubator is fixedly connected with a temperature control device, the rear side of the incubator is fixedly connected with an air blowing device, the front side of the incubator is rotatably connected with a cover, the incubator is evenly provided with support plates, the support plate is provided with a placement mechanism, the culture dish placed on the placement mechanism is fixed, the bottom end of the placement mechanism is fixedly connected with the top of the support plate, the two sides of the support plate are fixedly connected with shock absorbing mechanisms, and the shock absorbing mechanism is fixedly connected with the inner wall of the incubator. The present invention provides a temperature-adjustable biological cell engineering culture device and a method of using the same, and solves the problem of shaking and collision of the culture dish in the incubator when the cover of the incubator is opened.

Description

Technical Field

[0001] The invention relates to the technical field of cell engineering, and in particular to a temperature-adjustable biological cell engineering culture device and a use method thereof. Background Art

[0002] Cell engineering is an important aspect of bioengineering. It applies the theories and methods of cell biology and molecular biology to carry out genetic operations at the cellular level and large-scale cell and tissue culture according to people's design blueprints. The main technical fields currently involved in cell engineering include cell culture, cell fusion, cell splitting, chromosome manipulation and gene transfer. Through cell engineering, useful biological products can be produced or valuable plants can be cultivated, and new species or strains can be generated.

[0003] When conducting cell experiments, culture dishes need to be placed in an incubator. When the lid is opened or closed, the incubator will vibrate. Since the support plate is generally disassembled and washed for easy cleaning and disinfection, the support plate will vibrate. When the support plate vibrates, the culture dishes placed on the support plate will shake, and the culture dishes may collide with each other and break. Summary of the invention

[0004] 1. Technical issues to be solved

[0005] In view of the deficiencies in the prior art, the present invention provides a temperature-adjustable biological cell engineering culture device and a method for using the same, which solves the problem of shaking and colliding of culture dishes in the culture box when the cover of the culture box is opened.

[0006] (II) Technical solution

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions: a temperature-adjustable biological cell engineering culture device, including an incubator, a temperature control device, an air blowing device, a box cover, a placement mechanism, a shock absorbing mechanism, and a support plate. The top of the incubator is fixedly connected to the temperature control device, the rear side of the incubator is fixedly connected to the air blowing device, the front side of the incubator is rotatably connected to the box cover, support plates are evenly arranged in the incubator, a placement mechanism is arranged on the support plate, the bottom end of the placement mechanism is fixedly connected to the top of the support plate, the two sides of the support plate are fixedly connected to the shock absorbing mechanism, and the shock absorbing mechanism is fixedly connected to the inner wall of the incubator.

[0008] Preferably, one end of the shock absorbing mechanism close to the blowing device passes through the incubator and is fixedly connected to the blowing device, and the placement mechanisms are evenly arranged on the top of the support plate, and there is a certain distance between the placement mechanisms.

[0009] Preferably, the placement mechanism includes a support block fixedly connected to the top of the support plate, strip openings are evenly arranged on the outside of the support block, a support rod is slidably connected in the opening, an arc-shaped fixed disk is fixedly connected to the top of the support rod, an arc-shaped cross-shaped elastic airbag is fixedly connected between the support blocks, a cross support bar is fixedly connected to the top of the arc-shaped cross-shaped elastic airbag, an arc-shaped fixed disk is fixedly connected to the top of the four sides of the cross support bar, a support spring is fixedly connected to the bottom end of the middle of the support block, an arc-shaped connecting rod is fixedly connected near the bottom end of the support block between adjacent support blocks, and the outer side of the arc-shaped connecting rod is fixed A first electromagnet is connected, an L-shaped sliding rod is provided below the bottom end of the arc-shaped connecting rod, the L-shaped sliding rod is slidably connected to the top end of the support plate, a second electromagnet is fixedly connected to the side of the straight rod part of the L-shaped sliding rod close to the support block, a buffer rod is fixedly connected to the side of the top end of the straight rod of the L-shaped sliding rod close to the support block, an arc plate is fixedly connected to the other side of the buffer rod, fixed balls are fixedly connected to both ends of the arc plate, a wavy buffer plate is fixedly connected to the side of the top end of the straight rod of the L-shaped sliding rod away from the support block, an annular airbag is provided in the support block, and the bottom end of the support rod is fixedly connected to the top end of the annular airbag.

[0010] Preferably, the L-shaped sliding rod is L-shaped, and an arc-shaped fixing plate is fixedly connected to the top middle part of the support block. The arc-shaped fixing plate arranged at the top middle part of the support block is slightly lower than the arc-shaped fixing plate arranged on the support rod and the cross support bar. The arc-shaped plate is arc-shaped, and one side of the arc-shaped recess of the arc-shaped plate faces the direction of the cross support bar.

[0011] Preferably, the shock absorbing mechanism includes spherical supporting capsules evenly arranged on both sides of the supporting plate and fixedly connected to the supporting plate, the top and bottom ends of the spherical supporting capsules are provided with partition bars, the partition bars are fixedly connected to the inner wall of the incubator, an arc-shaped rubber sheet is fixedly connected between the end of the partition bar away from the incubator and the supporting plate, an air guide tube is fixedly connected to the side of the spherical supporting capsule away from the supporting plate, the side of the air guide tube away from the spherical supporting capsule is placed in the inner wall of the incubator, the spherical supporting capsule passes through the inner wall of the incubator and is fixedly connected to the air supply end of the blowing device, an air outlet pipe is fixedly connected to the front end of the partition bar, the air outlet pipe is fixedly connected to the inner walls of both sides of the incubator, an air inlet pipe is provided at the top and bottom ends of the air outlet pipe, the air inlet pipe is fixedly connected to the inner walls of the top and bottom ends of the incubator, the air inlet pipe passes through the inner wall between the top and bottom ends of the incubator and is fixedly connected to the air extraction end of the blowing device, an air barrier strip is provided on the inner side of the air inlet pipe, and the top end of the air barrier strip is fixedly connected to the inner wall of the incubator.

[0012] Preferably, circular air outlets are provided on the outer side of the spherical support bag near the top and bottom ends, the arc-shaped rubber sheet is arc-shaped, the air barrier strip is placed between the air inlet pipe and the support plate, the air outlet pipe is slightly longer than the support plate, a strip air outlet is provided on the side of the air outlet pipe away from the inner wall of the incubator, and a strip air inlet is provided on the end of the air inlet pipe away from the incubator.

[0013] A method for using a temperature-adjustable biological cell engineering culture device comprises the following steps:

[0014] S1: When the box cover is opened, the air blowing device is started, and air is filled into the air guide tube through the air supply end of the air blowing device. The air entering the spherical support bag enters the spherical support bag to expand the spherical support bag. When the spherical support bag expands, the air in the spherical support bag enters between the partition bar and the arc-shaped rubber sheet through the circular air outlet on the spherical support bag, so that the arc-shaped rubber sheet expands to the side away from the incubator to fix the support plate;

[0015] S2: When the spherical support bag and the arc-shaped rubber sheet expand, the excess air in the partition strip and the arc-shaped rubber sheet is ejected from the strip-shaped air outlet on the air outlet pipe, and the air extraction end of the air extraction device sucks the air flow ejected from the air outlet pipe into the air inlet pipe, so that a flowing air curtain is formed between the air outlet pipe and the air inlet pipe, preventing the outside air with different temperatures from the incubator from entering the incubator and affecting the temperature in the incubator, and reducing the temperature loss in the incubator by setting the air separation strip between the air inlet pipe and the support plate;

[0016] S3: When the box cover is opened, the current passed into the second electromagnet is changed so that the magnetic poles of the second electromagnet and the first electromagnet are the same, and the L-shaped sliding rod is away from the support block, so that the culture dish is placed on the arc-shaped fixed plate. When the culture dish is placed on the top of the arc-shaped fixed plate, the culture dish is fixed by multiple arc-shaped fixed plates. When the temperature in the incubator changes, the annular airbag and the arc-shaped cross-shaped elastic airbag will expand and contract with the change in temperature so that the top of the arc-shaped fixed plate can always be close to the bottom of the culture dish to fix the culture dish. When the box cover is closed, the current passed into the second electromagnet is changed so that the magnetic poles of the second electromagnet and the first electromagnet are opposite. Under the action of the magnetic force, the L-shaped sliding rod slides to the side where the support block is located, and the sliding of the L-shaped sliding rod drives the fixed ball to slide to fix the outer edge of the culture dish.

[0017] (III) Beneficial effects

[0018] (1) The present invention prevents the culture dish from slipping out of the hands and colliding with the support plate between the support block and causing the culture dish to be broken during the placement process by means of the support block, the support rod, the arc-shaped fixing plate, the arc-shaped cross-shaped elastic airbag, and the annular airbag arranged in the placement mechanism. In addition, the annular airbag arranged in the support block enables the arc-shaped fixing plate to always fit the bottom of the culture dish to fix the culture dish when the temperature in the incubator changes, thereby preventing the culture dishes from colliding and breaking when the support plate vibrates.

[0019] (2) The present invention fixes the outer edge of the culture dish placed on the arc-shaped fixed plate by means of an arc-shaped connecting rod, a first electromagnet, an L-shaped sliding rod, a second electromagnet, a buffer rod, an arc-shaped plate, and a fixing ball arranged in the placement mechanism, thereby enhancing the fixing effect of the culture dish placed on the arc-shaped fixed plate and preventing the culture dish from shaking left and right and colliding with adjacent culture dishes so as to break the culture dish and affect the normal cell engineering experiment.

[0020] (3) The present invention, through the spherical support bag, the spacer, the arc-shaped rubber sheet, the air guide tube and the blowing device arranged in the shock-absorbing mechanism, can fix the support plate by the expansion of the spherical support bag and the expansion of the arc-shaped rubber sheet outward when the box cover is opened or closed, so as to prevent the support plate from colliding with the wall of the incubator when the cover is opened and closed, thereby preventing the culture dish placed on the support plate from shaking and colliding.

[0021] (4) The present invention prevents the outside air with a temperature different from that in the incubator from entering the incubator when the lid is opened, thereby causing a significant change in the temperature in the incubator and affecting normal cell engineering experiments, by means of an air duct, an air outlet duct, an air inlet duct, and an air barrier strip disposed in the shock absorbing mechanism. The air barrier strip disposed between the air inlet duct and the support plate reduces the loss of temperature in the incubator. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the structure of the present invention;

[0023] Figure 2 This is a schematic diagram of the internal structure of the incubator of the present invention;

[0024] Figure 3 It is a front cross-sectional view of the incubator of the present invention;

[0025] Figure 4 It is a schematic diagram of the structure of the shock absorbing mechanism of the present invention;

[0026] Figure 5 It is a schematic diagram of the placement mechanism structure of the present invention;

[0027] Figure 6 It is a front cross-sectional view of the support block of the present invention;

[0028] Figure 7 For the present invention Figure 3 A magnified view of the structure at center;

[0029] Figure 8 For the present invention Figure 4 Enlarged view of the structure at point B in the middle.

[0030] Among them, the incubator 1, the temperature control device 2, the blowing device 3, the box cover 4, the placement mechanism 5, the support block 501, the support rod 502, the arc-shaped fixed plate 503, the arc-shaped cross-shaped elastic airbag 504, the cross support bar 505, the support spring 506, the arc-shaped connecting rod 507, the first electromagnet 508, the L-shaped sliding rod 509, the second electromagnet 510, the buffer rod 511, the arc-shaped plate 512, the fixed ball 513, the wavy buffer plate 514, the annular airbag 515, the shock absorbing mechanism 6, the spherical support bag 601, the partition bar 602, the arc-shaped rubber sheet 603, the air guide tube 604, the air outlet pipe 605, the air inlet pipe 606, the air partition strip 607, and the support plate 7. DETAILED DESCRIPTION

[0031] The following will be combined with the accompanying drawings of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] like Figure 1-8 As shown, an embodiment of the present invention provides a temperature-adjustable biological cell engineering culture device, including a culture box 1, a temperature control device 2, an air blowing device 3, a box cover 4, a placement mechanism 5, a shock absorbing mechanism 6, and a support plate 7. The temperature control device 2 is fixedly connected to the top of the culture box 1, the air blowing device 3 is fixedly connected to the rear side of the culture box 1, and the box cover 4 is rotatably connected to the front side of the culture box 1. Support plates 7 are evenly arranged in the culture box 1, and placement mechanisms 5 are arranged on the support plates 7. The bottom end of the placement mechanism 5 is fixedly connected to the top of the support plate 7, and shock absorbing mechanisms 6 are fixedly connected to both sides of the support plate 7. The shock absorbing mechanism 6 is fixedly connected to the inner wall of the culture box 1, and one end of the shock absorbing mechanism 6 close to the air blowing device 3 passes through the culture box 1 and is fixedly connected to the air blowing device 3. The placement mechanisms 5 are evenly arranged on the top of the support plate 7, and there is a certain distance between the placement mechanisms 5.

[0033] The placing mechanism 5 includes a supporting block 501 fixedly connected to the top of the supporting plate 7, the outer side of the supporting block 501 is evenly provided with a strip opening, a supporting rod 502 is slidably connected in the opening, an arc-shaped fixed disk 503 is fixedly connected to the top of the supporting rod 502, an arc-shaped cross-shaped elastic airbag 504 is fixedly connected between the supporting blocks 501, a cross supporting bar 505 is fixedly connected to the top of the arc-shaped cross-shaped elastic airbag 504, and an arc-shaped fixed disk 503 is fixedly connected to the top of the four sides of the cross supporting bar 505, a supporting spring 506 is fixedly connected to the bottom end of the middle part of the supporting block 501, an arc connecting rod 507 is fixedly connected near the bottom end of the supporting block 501 between adjacent supporting blocks 501, a first electromagnet 508 is fixedly connected to the outer side of the arc connecting rod 507, an L-shaped sliding rod 509 is provided below the bottom end of the arc connecting rod 507, the L-shaped sliding rod 509 is slidably connected to the top of the supporting plate 7, and the straight rod part of the L-shaped sliding rod 509 is close to the supporting block 5 A second electromagnet 510 is fixedly connected to one side of 01, a buffer rod 511 is fixedly connected to the top of the L-shaped sliding rod 509 on the side close to the support block 501, and an arc plate 512 is fixedly connected to the other side of the buffer rod 511. Fixed balls 513 are fixedly connected to both ends of the arc plate 512. A wavy buffer plate 514 is fixedly connected to the top of the L-shaped sliding rod 509 away from the support block 501. An annular air bag 515 is arranged in the support block 501, and the bottom end of the support rod 502 is fixedly connected to the top of the annular air bag 515. The L-shaped sliding rod 509 is L-shaped, and an arc-shaped fixed disk 503 is fixedly connected to the top of the middle part of the support block 501. The arc-shaped fixed disk 503 arranged at the top of the middle part of the support block 501 is slightly lower than the arc-shaped fixed disk 503 arranged on the support rod 502 and the cross support bar 505. The arc plate 512 is arc-shaped, and one side of the arc-shaped recessed arc plate 512 faces the direction of the cross support bar 505.

[0034] The shock absorbing mechanism 6 includes a spherical support capsule 601 which is evenly arranged on both sides of the support plate 7 and fixedly connected to the support plate 7. The top and bottom ends of the spherical support capsule 601 are provided with a partition bar 602, and the partition bar 602 is fixedly connected to the inner wall of the incubator 1. An arc-shaped rubber sheet 603 is fixedly connected between the end of the partition bar 602 away from the incubator 1 and the support plate 7. An air guide tube 604 is fixedly connected to the side of the spherical support capsule 601 away from the support plate 7. The side of the air guide tube 604 away from the spherical support capsule 601 is placed in the inner wall of the incubator 1. The spherical support capsule 601 passes through the inner wall of the incubator 1 and is fixedly connected to the air supply end of the blowing device 3. An air outlet pipe 605 is fixedly connected to the front end of the partition bar 602. The air outlet pipe 605 is fixedly connected to the inner walls of the two sides of the incubator 1. An air inlet pipe 606 is provided at the top and bottom of the air outlet pipe 605. The air inlet pipe 606 is connected to the incubator 1. The inner walls of the top and bottom ends of the incubator 1 are fixedly connected, the air inlet pipe 606 passes through the inner wall between the top and bottom ends of the incubator 1 and is fixedly connected to the exhaust end of the blowing device 3, an air barrier strip 607 is provided on the inner side of the air inlet pipe 606, the top of the air barrier strip 607 is fixedly connected to the inner wall of the top end of the incubator 1 inside 606, a circular air outlet is provided on the outer side of the spherical support capsule 601 near the top and the bottom, the arc-shaped rubber sheet 603 is arc-shaped, the air barrier strip 607 is placed between the air inlet pipe 606 and the support plate 7, the support plate 7 is arranged in the incubator 1 on the inner side of the air barrier strip, the air outlet pipe 605 is slightly longer than the support plate 7, a strip air outlet is provided on the side of the air outlet pipe 605 away from the inner wall of the incubator 1, a strip air inlet is provided on the end of the air inlet pipe 606 away from the incubator 1, the air barrier strip 607 has a certain flexibility and there is a certain gap between the air barrier strips 607.

[0035] A method for using a temperature-adjustable biological cell engineering culture device comprises the following steps:

[0036] S1: When the box cover 4 is opened, the air blowing device 3 is started, and air is filled into the air guide tube 604 through the air supply end of the air blowing device 3, and the air expands the spherical support capsule 601. When the spherical support capsule 601 expands, the air in the spherical support capsule 601 enters between the partition bar 602 and the arc-shaped rubber sheet 603 through the circular air outlet on the spherical support capsule 601, so that the arc-shaped rubber sheet 603 expands to the side away from the incubator 1 to fix the support plate 7;

[0037] S2: When the spherical support bag 601 and the arc-shaped rubber sheet 603 expand, the excess air in the partition strip 602 and the arc-shaped rubber sheet 603 is ejected from the strip-shaped air outlet on the air outlet pipe 605, and the air extraction end of the air extraction device sucks the air flow ejected from the air outlet pipe 605 into the air inlet pipe 606, so that a flowing air curtain is formed between the air outlet pipe 605 and the air inlet pipe 606, preventing the outside air with different temperatures from the incubator 1 from entering the incubator 1 and affecting the temperature in the incubator 1, and reducing the temperature loss in the incubator 1 too fast by the air separation strip 607 arranged between the air inlet pipe 606 and the support plate 7;

[0038] S3: When the box cover 4 is opened, the current passed into the second electromagnet 510 is changed so that the magnetic poles of the second electromagnet 510 and the first electromagnet 508 are the same, and the L-shaped sliding rod 509 is away from the support block 501, so that it is convenient to place the culture dish on the arc-shaped fixed disk 503. When the culture dish is placed on the top of the arc-shaped fixed disk 503, the culture dish is fixed by multiple arc-shaped fixed disks 503. When the temperature in the incubator 1 changes, the annular airbag 515 and the arc-shaped cross-shaped elastic airbag 504 will expand and contract with the change of temperature so that the top of the arc-shaped fixed disk 503 can always be close to the bottom of the culture dish to fix the culture dish. When the box cover 4 is closed, the current passed into the second electromagnet 510 is changed so that the magnetic poles of the second electromagnet 510 and the first electromagnet 508 are opposite. Under the action of magnetic force, the L-shaped sliding rod 509 slides to the side where the support block 501 is located, and the sliding of the L-shaped sliding rod 509 drives the fixed ball 513 to slide to fix the outer edge of the culture dish.

[0039] During use, when the box cover 4 is opened or closed, the incubator 1 will vibrate. Since the support plate 7 is generally detachable and washable for easy cleaning and disinfection, the support plate 7 will vibrate. When the support plate 7 vibrates, the culture dishes placed on the support plate 7 will shake, and the culture dishes may collide with each other. When the box cover 4 is opened, the blowing device 3 is started, and the blowing device 3 blows air into the air guide tube 604 through the air supply end. The air entering the air guide tube 604 enters the partition bar 602 to expand the spherical support bag 601, and the spherical support bag 601 is inflated. After the support capsule 601 is expanded, the air in the spherical support capsule 601 is discharged into the partition bar 602 through the circular air outlet arranged on the spherical support capsule 601. The air entering the partition bar 602 makes the arc-shaped rubber sheet 603 expand toward the side away from the incubator 1. The expansion of the spherical support capsule 601 and the arc-shaped rubber sheet 603 fixes the support plate 7 to prevent the support plate 7 from vibrating when the box cover 4 is opened, causing the culture dish placed on the support plate 7 to shake and collide. The expansion of the spherical support capsule 601 prevents the support plate 7 from colliding with the culture dish when the box cover 4 is opened. The collision between the inner walls of the box 1 causes the culture dishes on the support plate 7 to move and collide with each other under the action of inertia, resulting in the phenomenon that the culture dishes are broken, and the air between the partition bars 602 enters the air outlet pipe 605 through the front end of the partition bar 602 and is ejected from the strip-shaped air outlet on the air outlet pipe 605. Under the action of the air inlet pipe 606 and the air extraction end of the blowing device 3, the air ejected from the air outlet pipe 605 is sucked into the air inlet pipe 606, so that a flowing air curtain is formed between the air outlet pipe 605 and the air inlet pipe 606. When the box cover 4 is opened, excessive outside air and the culture dishes are Air with different temperatures in the incubator 1 enters the incubator 1, affecting the temperature inside the incubator 1. The air barrier 607 arranged between the air inlet pipe 606 and the support plate 7 reduces the temperature loss in the incubator 1, and reduces the influence of the flowing air curtain between the air outlet pipe 605 and the air inlet pipe 606 on the temperature inside the incubator 1. When the box cover 4 is closed, the blowing device 3 stops working, and the spherical support bag 601 gradually shrinks. During the shrinking process, the spherical support bag 601 also has a certain buffering effect, thereby preventing the support plate 7 from vibrating when the box cover 4 is closed.

[0040] When the box cover 4 is opened to place the culture dish on the support plate 7, the direction of the current passed through the second electromagnet 510 is changed so that the magnetic poles of the second electromagnet 510 and the first electromagnet 508 are the same, so that the L-shaped sliding rod 509 slides on the support plate 7, so that the fixing ball 513 is away from the support block 501, which is convenient for placing the culture dish on the arc-shaped fixing plate 503. When the culture dish is placed on the arc-shaped fixing plate 503, the arrangement of multiple arc-shaped fixing plates 503 prevents the culture dish from sliding on the arc-shaped fixing plate 503 when the support plate 7 vibrates. When the temperature in the incubator 1 decreases, the annular airbag 515 arranged in the support block 501 contracts due to the cold, so that the support rod 509 is 02 descends so that the arc-shaped fixing plate 503 arranged at the top of the support block 501 can contact the bottom of the culture dish to enhance the anti-skid effect of the culture dish, thereby preventing the culture dishes from colliding with each other when they are in a low temperature state for a long time, and making it easier to break. The arc-shaped cross-shaped elastic airbag 504 arranged between the support blocks 501 prevents the culture dish from falling out of the hand if the hand slips when placing the culture dish, and prevents the edge of the culture dish from colliding with the support plate 7 between the support blocks 501 to cause the culture dish to break. When the temperature in the incubator 1 changes, the arc-shaped cross-shaped elastic airbag 504 will change together with the annular airbag 515 to The arc-shaped fixing plate 503 placed above the arc-shaped cross-shaped elastic airbag 504 can always fit in the middle of the bottom end of the culture dish to fix the culture dish, thereby enhancing the fixing effect of the culture dish. When the box cover 4 is closed, the current passed into the second electromagnet 510 changes so that the magnetic poles of the second electromagnet 510 and the first electromagnet 508 are opposite. Under the action of the magnetic force, the L-shaped sliding rod 509 slides toward the side where the support block 501 is located. The sliding of the L-shaped sliding rod 509 drives the fixing ball 513 to move toward the side where the support block 501 is located, so that the fixing ball 513 is closely attached to the surface of the culture dish to fix the outer edge of the culture dish, thereby enhancing the fixation of the culture dish. The arc plate 512 and the buffer rod 511 arranged at the top of the L-shaped sliding rod 509 can buffer the impact force on the culture dish when the fixed ball 513 contacts the culture dish, preventing the fixed ball 513 from approaching the edge of the culture dish at a high speed due to the influence of the magnetic force and breaking the culture dish. The wavy buffer plate 514 arranged on the side of the L-shaped sliding rod 509 away from the support block 501 can buffer the collision between the L-shaped sliding rods 509, preventing the L-shaped sliding rod 509 from colliding with the adjacent L-shaped sliding rod 509 when sliding toward the side away from the support block 501, causing the culture dish placed on the arc-shaped fixed plate 503 to shake.

Claims

1. A temperature-adjustable biological cell engineering culture device, comprising a culture box (1), a temperature control device (2), an air blowing device (3), a box cover (4), a placement mechanism (5), a shock absorbing mechanism (6), and a support plate (7), characterized in that: The top of the incubator (1) is fixedly connected to a temperature control device (2), the rear side of the incubator (1) is fixedly connected to an air blowing device (3), the front side of the incubator (1) is rotatably connected to a box cover (4), support plates (7) are evenly arranged inside the incubator (1), a placement mechanism (5) is arranged on the support plate (7), the bottom end of the placement mechanism (5) is fixedly connected to the top of the support plate (7), shock absorbing mechanisms (6) are fixedly connected to both sides of the support plate (7), and the shock absorbing mechanisms (6) are fixedly connected to the inner wall of the incubator (1); The end of the shock absorbing mechanism (6) close to the air blowing device (3) passes through the incubator (1) and is fixedly connected to the air blowing device (3); the placement mechanisms (5) are evenly arranged on the top of the support plate (7), and there is a certain distance between the placement mechanisms (5); The placement mechanism (5) comprises a support block (501) fixedly connected to the top of the support plate (7); strip openings are evenly arranged on the outside of the support block (501); a support rod (502) is slidably connected in the opening; an arc-shaped fixed disk (503) is fixedly connected to the top of the support rod (502); an arc-shaped cross-shaped elastic airbag (504) is fixedly connected between the support blocks (501); a cross support bar (505) is fixedly connected to the top of the arc-shaped cross-shaped elastic airbag (504); the arc-shaped fixed disk (503) is fixedly connected to the top of the four sides of the cross support bar (505); a support spring (506) is fixedly connected to the bottom of the middle part of the support block (501); an arc-shaped connecting rod (507) is fixedly connected between adjacent support blocks (501) near the bottom of the support block (501); and a first electromagnet (507) is fixedly connected to the outside of the arc-shaped connecting rod (507). 08), an L-shaped sliding rod (509) is provided below the bottom end of the arc-shaped connecting rod (507), the L-shaped sliding rod (509) is slidably connected to the top end of the support plate (7), a second electromagnet (510) is fixedly connected to the side of the straight rod portion of the L-shaped sliding rod (509) close to the support block (501), a buffer rod (511) is fixedly connected to the side of the top end of the straight rod of the L-shaped sliding rod (509) close to the support block (501), an arc-shaped plate (512) is fixedly connected to the other side of the buffer rod (511), fixed balls (513) are fixedly connected to both ends of the arc-shaped plate (512), a wavy buffer plate (514) is fixedly connected to the side of the top end of the straight rod of the L-shaped sliding rod (509) away from the support block (501), an annular air bag (515) is provided in the support block (501), and the bottom end of the support rod (502) is fixedly connected to the top end of the annular air bag (515); The shock absorbing mechanism (6) comprises spherical support capsules (601) evenly arranged on both sides of the support plate (7) and fixedly connected to the support plate (7); the top and bottom ends of the spherical support capsule (601) are provided with partition bars (602); the partition bars (602) are fixedly connected to the inner wall of the incubator (1); an arc-shaped rubber sheet (603) is fixedly connected between the end of the partition bar (602) away from the incubator (1) and the support plate (7); an air guide tube (604) is fixedly connected to the side of the spherical support capsule (601) away from the support plate (7); the side of the air guide tube (604) away from the spherical support capsule (601) is placed in the inner wall of the incubator (1); and the spherical support capsule (601) is fixedly connected to the support plate (7); 01) passes through the inner wall of the incubator (1) and is fixedly connected to the air supply end of the air blowing device (3); an air outlet pipe (605) is fixedly connected to the front end of the partition bar (602); the air outlet pipe (605) is fixedly connected to the inner walls of both sides of the incubator (1); an air inlet pipe (606) is provided at the top and bottom ends of the air outlet pipe (605); the air inlet pipe (606) is fixedly connected to the inner walls of the top and bottom ends of the incubator (1); the air inlet pipe (606) passes through the inner wall between the top and bottom ends of the incubator (1) and is fixedly connected to the air extraction end of the air blowing device (3); an air partition bar (607) is provided on the inner side of the air inlet pipe (606); the top end of the air partition bar (607) is fixedly connected to the inner wall of the incubator (1).

2. The temperature-adjustable biological cell engineering culture device according to claim 1, characterized in that: The L-shaped sliding rod (509) is L-shaped, and an arc-shaped fixing plate (503) is fixedly connected to the top of the middle part of the support block (501). The arc-shaped fixing plate (503) arranged at the top of the middle part of the support block (501) is slightly lower than the arc-shaped fixing plate (503) arranged on the support rod (502) and the cross support bar (505). The arc-shaped plate (512) is arc-shaped, and one side of the arc-shaped concave of the arc-shaped plate (512) faces the direction where the cross support bar (505) is located.

3. The temperature-adjustable biological cell engineering culture device according to claim 2, characterized in that: The outer side of the spherical support bag (601) is provided with circular air outlets near the top and bottom ends, the arc-shaped rubber sheet (603) is arc-shaped, the air barrier strip (607) is placed between the air inlet pipe (606) and the support plate (7), the air outlet pipe (605) is slightly longer than the support plate (7), a strip-shaped air outlet is provided on the side of the air outlet pipe (605) away from the inner wall of the incubator (1), and a strip-shaped air inlet is provided on the end of the air inlet pipe (606) away from the incubator (1).

4. A method for using a temperature-adjustable biological cell engineering culture device according to claim 3, characterized in that: The following steps are included: S1: When the box cover (4) is opened, the air blowing device (3) is started, and air is filled into the air guide tube (604) through the air supply end of the air blowing device (3), and the air entering the spherical support capsule (601) enters the spherical support capsule (601) to expand the spherical support capsule (601). When the spherical support capsule (601) expands, the air in the spherical support capsule (601) enters between the partition bar (602) and the arc-shaped rubber sheet (603) through the circular air outlet on the spherical support capsule (601), so that the arc-shaped rubber sheet (603) expands toward the side away from the incubator (1) to fix the support plate (7); S2: When the spherical support bag (601) and the arc-shaped rubber sheet (603) expand, the excess air in the partition strip (602) and the arc-shaped rubber sheet (603) is ejected from the strip-shaped air outlet on the air outlet pipe (605), and the air extraction end of the air extraction device sucks the air flow ejected from the air outlet pipe (605) into the air inlet pipe (606), so that a flowing air curtain is formed between the air outlet pipe (605) and the air inlet pipe (606), thereby preventing the outside air with a temperature different from that in the incubator (1) from entering the incubator (1) and affecting the temperature in the incubator (1), and reducing the temperature loss in the incubator (1) by the air separation strip (607) provided between the air inlet pipe (606) and the support plate (7); S3: When the box cover (4) is opened, the current flowing into the second electromagnet (510) is changed so that the magnetic poles of the second electromagnet (510) and the first electromagnet (508) are the same, so that the L-shaped sliding rod (509) is away from the support block (501), so that the culture dish can be placed on the arc-shaped fixed plate (503) conveniently. When the culture dish is placed on the top of the arc-shaped fixed plate (503), the culture dish is fixed by the multiple arc-shaped fixed plates (503). When the temperature in the incubator (1) changes, the annular airbag (515) and the arc-shaped cross-shaped elastic airbag (50 4) The arc-shaped fixed plate (503) expands and contracts with the change of temperature so that the top end of the arc-shaped fixed plate (503) can always be in close contact with the bottom end of the culture dish to fix the culture dish. When the box cover (4) is closed, the current passed into the second electromagnet (510) changes so that the magnetic poles of the second electromagnet (510) and the first electromagnet (508) are opposite. Under the action of the magnetic force, the L-shaped sliding rod (509) slides toward the side where the support block (501) is located. The sliding of the L-shaped sliding rod (509) drives the fixed ball (513) to slide to fix the outer edge of the culture dish.

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