A method for extracting and applying Erjingfang polysaccharide

Abstract

The present invention discloses an extraction method and application of Erjingfang polysaccharide. The extraction method of Erjingfang polysaccharide of the present invention comprises the following steps: step S1, mixing wolfberry powder and polygonatum powder, adding a first ethanol solution for reflux treatment, and obtaining a defatted medicinal material powder; step S2, adding water to the defatted medicinal material powder at a solid-liquid ratio of 1g:10-30mL, after soaking and ultrasonic treatment, suction filtering and collecting the filtrate, and then adding a second alcohol ethanol solution for alcohol precipitation treatment after concentration, collecting the precipitate, and drying to obtain Erjingfang polysaccharide. The experiment shows that the Erjingfang polysaccharide prepared by the present invention has excellent antioxidant and anti-fatigue effects, which can make it have broad prospects in the fields of health care products and cosmetics. In addition, the polysaccharide extraction process of the present invention is simple, and the extraction time is short, the cost is low, and the efficiency is high. It can be used for industrial production and provide technical support for industrial production related products.

Description

Technical Field

[0001] The invention relates to a polysaccharide extraction process, in particular to an extraction method and application of Erjingfang polysaccharide. Background Art

[0002] Polysaccharides are a very important class of natural macromolecules in higher plants, animals, and microbial cells. They are a natural biomacromolecule formed by the polymerization of more than 10 monosaccharides through α- or β-glycosidic bonds. The molecular weight ranges from tens of thousands to millions. They are expressed in the general formula (C6H 10 O5) n It is widely present in plants, animals and microorganisms, and is an important component of higher plant and animal cell membranes and microbial cell walls. Polysaccharides are usually composed of monosaccharides such as xylose, arabinose, ribose, rhamnose, fructose, fucose, mannose, galactose, glucose and glucuronic acid. Plant polysaccharides are rich in sources, and finding functional ingredients from natural plants is a current research hotspot. In recent years, reports on the biological functions of active polysaccharides mainly include polysaccharides' antioxidant, anti-tumor, anti-coagulation, lipid-lowering, anti-viral, and immune function enhancement. In addition to being used as drugs for treatment, Chinese medicine polysaccharides also have the effects of delaying aging, whitening, after-sun repair, acne and anti-inflammatory, and promoting wound healing in the field of skin. There are many types of plant polysaccharides, and they are non-cytotoxic and highly safe for use in organisms, so they have been better promoted and applied in the pharmaceutical and health care products industries.

[0003] Polygonatum sibiricum is the dried rhizome of Polygonatum kingianum Coll.et Hemsl., Polygonatum sibiricum Red. or Polygonatum cyrtonema Hua, all of the Liliaceae family. It has the effects of nourishing qi and yin, strengthening the spleen, moistening the lungs and benefiting the kidneys. Polygonatum sibiricum has a variety of chemical components, mainly including polysaccharides, steroidal saponins, flavonoids, phenylpropanoids, alkaloids and other components. As one of the main active ingredients of Polygonatum sibiricum, Polygonatum sibiricum polysaccharide has pharmacological effects such as enhancing immune function, inhibiting bacteria and inflammation, and regulating blood sugar and blood lipids. It is also the only quality control component of Polygonatum sibiricum specified in the 2020 edition of the Chinese Pharmacopoeia. Wang Yan et al. obtained the purified product PSP-1a of Polygonatum sibiricum polysaccharide through separation and purification. It is mainly composed of galactose, arabinose, rhamnose, xylose and glucose, accounting for 0.18%, 1.50%, 97.25%, 0.77% and 0.30% respectively. Tao Aien et al. analyzed the polysaccharide composition of medicinal polygonatum from three sources. Through pattern recognition, they found that the common monosaccharide components in the three polygonatum polysaccharides are D-mannose, D-ribose, L-rhamnose and other 7 monosaccharides and uronic acid.

[0004] Lycium barbarum L., the dried mature fruit of Lycium barbarum L., is a traditional Chinese food ingredient that is both edible and medicinal. It has the effects of improving eyesight, strengthening the body, nourishing yin and moistening the lungs. Lycium barbarum polysaccharide is one of the main active ingredients in Lycium barbarum, accounting for 5-8% of the dried fruit and 1.02-2.48% of the raw material. It has many effects such as lowering blood sugar, anti-oxidation, anti-tumor, anti-aging, neuroprotection, and immune regulation. More than 40 polysaccharides can be isolated from the fruit, with a molecular weight range of 8-241kDa. They include galacturonic acid, 18 amino acids and 9 monosaccharides, namely xylose (Xyl), glucose (Glc), arabinose (Ara), rhamnose (Rha), mannose (Man), galactose (Gal), fucose (Fuc), galacturonic acid (GalA), and glucuronic acid (GlcA).

[0005] The prescription composed of Polygonatum sibiricum and Lycium barbarum is also known as Erjing Pills, which comes from "Volume 21 of Miraculous Prescriptions: Various Voids". In addition, "Shengji Zonglu" mentioned this prescription: "Regular use can help Qi and replenish essence, replenish Dantian, activate blood circulation and keep the face, and live forever." Polygonatum sibiricum and Lycium barbarum in the prescription are both Chinese medicines with medicinal and edible origins. Polygonatum sibiricum nourishes the kidney and moisturizes dryness, replenishes the spleen and replenishes Qi, and is an important medicine for replenishing Qi and Yin; Lycium barbarum nourishes the liver and kidney, replenishes essence and improves eyesight, and is an important medicine for balancing the yin and yang of the liver and kidney. The two medicines are used together, and the effect of nourishing Yin, replenishing kidney and replenishing essence is particularly good. Modern studies have shown that the total polysaccharide of Erjing Pills can resist vascular dementia, improve the learning and memory ability of rats with kidney yin deficiency model, and reduce blood sugar and blood lipids in type 2 diabetes. However, there are still few studies on the polysaccharide extraction of Erjing Pills, and there are still problems such as low extraction content, long extraction time, and more pigments. In addition, there are still few studies on the antioxidant and anti-fatigue effects of Erjing Pills. CN113509524A discloses a method for preparing a medicine of Erjingfang Polygonatum and Lycium barbarum for preventing and treating type II diabetes, but the polysaccharide extraction method thereof takes a long time and has low efficiency.

[0006] Therefore, the present invention will study the extraction process of polygonatum and wolfberry polysaccharides to improve the extraction efficiency and utilization rate of this prescription, and study its antioxidant and anti-fatigue effects to enrich the efficacy research data of its prescription. Summary of the invention

[0007] The present invention aims to solve at least one of the technical problems existing in the prior art. To this end, the present invention provides an extraction method and application of Erjingfang polysaccharide, the extraction method is simple and convenient, the extraction rate is high, and the extracted Erjingfang polysaccharide has excellent antioxidant and anti-fatigue effects.

[0008] The invention also provides a kind of dimethicone polysaccharide.

[0009] The invention also provides an application of Erjingfang polysaccharide in preparing products with antioxidant activity.

[0010] The invention also provides an application of Erjingfang polysaccharide in preparing anti-fatigue products.

[0011] The first aspect of the present invention provides a method for extracting polysaccharides from Erjingfang, comprising the following steps:

[0012] Step S1, mixing wolfberry powder and polygonatum sibiricum powder, adding a first ethanol solution for reflux treatment, and obtaining defatted medicinal material powder;

[0013] Step S2, adding water to the defatted medicinal material powder at a solid-liquid ratio of 1 g: 10-30 mL, after soaking and ultrasonic treatment, filtering and collecting the filtrate, then adding a second alcohol ethanol solution for alcohol precipitation after concentration, collecting the precipitate, and obtaining Erjingfang polysaccharide after drying.

[0014] The extraction method according to the embodiment of the present invention has at least the following beneficial effects:

[0015] (1) The present invention combines defatting technology, hot water extraction technology and ultrasound-assisted extraction technology, and optimizes the extraction process to effectively improve the extraction rate of Erjingfang polysaccharide.

[0016] (2) The polysaccharide extraction process of the present invention has simple steps, short extraction time, low cost and high efficiency, and can be used in industrial production to provide technical support for industrial production-related products.

[0017] In some embodiments of the present invention, the mass ratio of the wolfberry powder to the polygonatum powder is 1:0.8-1.2;

[0018] Preferably, the mass ratio of the wolfberry powder to the polygonatum powder is 1:1.

[0019] In some embodiments of the present invention, the concentration of ethanol in the first ethanol solution is greater than 98%;

[0020] Preferably, the first ethanol solution is anhydrous ethanol.

[0021] In some embodiments of the present invention, the mass volume ratio of the mixture of the wolfberry powder and the polygonatum powder to the first ethanol solution is 1 g: 8 to 12 mL;

[0022] Preferably, the mass volume ratio of the mixture of the wolfberry powder and the polygonatum powder to the first ethanol solution is 1 g:10 mL.

[0023] In some embodiments of the present invention, the reflux treatment time is 0.8h to 1.2h.

[0024] Preferably, the reflux treatment time is 1 hour.

[0025] In some embodiments of the present invention, in step S2, the material-liquid ratio is 1 g: 10-20 mL.

[0026] More preferably, the material-liquid ratio is 1 g: 10-15 mL;

[0027] In some embodiments of the present invention, in step S2, the soaking temperature is 55°C to 65°C;

[0028] Preferably, the soaking temperature is 60°C.

[0029] In some embodiments of the present invention, the soaking time is 20 min to 150 min;

[0030] Preferably, the soaking time is 30 min to 90 min;

[0031] More preferably, the soaking time is 30 min to 60 min.

[0032] In some embodiments of the present invention, the ultrasonic treatment time is 2 min to 65 min;

[0033] Preferably, the ultrasonic treatment time is 5 min to 55 min;

[0034] More preferably, the ultrasonic treatment time is 10 min to 55 min;

[0035] More preferably, the ultrasonic treatment time is 20 min to 55 min.

[0036] In some embodiments of the present invention, the solid-liquid ratio is 1 g:15 mL, the soaking time is 30 min, and the ultrasonic treatment time is 35 min.

[0037] In some embodiments of the present invention, the ultrasonic power of the ultrasonic treatment is 550W to 650W;

[0038] Preferably, the ultrasonic power of the ultrasonic treatment is 600W.

[0039] In the present invention, the cavitation effect, mechanical effect and thermal effect of ultrasound are utilized to accelerate the flow, penetration, dissolution and diffusion of the solvent, thereby achieving the purpose of improving the extraction efficiency of polysaccharides.

[0040] In some embodiments of the present invention, the concentration rate of the concentration is 8 to 12%;

[0041] Preferably, the concentration rate of the concentration is 10%.

[0042] In some embodiments of the present invention, in the alcohol precipitation treatment, the final concentration of the second alcohol ethanol solution is 85% to 95%;

[0043] Preferably, the final concentration of the second alcohol ethanol solution is 90%.

[0044] In some embodiments of the present invention, the alcohol precipitation treatment time is 8 to 36 hours;

[0045] Preferably, the alcohol precipitation treatment time is 8 to 12 hours.

[0046] In some embodiments of the present invention, the drying temperature is 55 to 65°C;

[0047] Preferably, the drying temperature is 60°C.

[0048] In some embodiments of the present invention, after step S2, the method further includes: mixing the dimethicone polysaccharide with water and activated carbon and performing a decolorization treatment.

[0049] In some embodiments of the present invention, the final concentration of the activated carbon is 0.0075-0.035 g / mL.

[0050] Preferably, the final concentration of the activated carbon is 0.0075 to 0.0175 g / mL;

[0051] More preferably, the final concentration of the activated carbon is 0.0075-0.01 g / mL.

[0052] In some embodiments of the present invention, the temperature of the decolorization treatment is 30°C to 80°C.

[0053] In some embodiments of the present invention, the decolorization treatment time is 10 min to 60 min.

[0054] The second aspect of the present invention provides a Erjingfang polysaccharide, which is prepared by the above-mentioned Erjingfang polysaccharide extraction method.

[0055] The Erjingfang polysaccharide according to the embodiment of the present invention has at least the following beneficial effects: the Erjingfang polysaccharide prepared by the above method has excellent antioxidant activity and anti-fatigue activity.

[0056] The third aspect of the present invention provides a use of Erjingfang polysaccharide in preparing products with antioxidant activity.

[0057] The fourth aspect of the present invention provides a use of Erjingfang polysaccharide in the preparation of anti-fatigue products.

[0058] Other features and advantages of the present invention will be set forth in the description which follows, and in part will be apparent from the description, or may be learned by practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0059] The present invention will be further described below with reference to the accompanying drawings and embodiments, wherein:

[0060] Figure 1 It is the glucose standard curve diagram of the present invention.

[0061] Figure 2 This is a statistical diagram of the effects of different solid-liquid ratios on polysaccharide extraction rates in the present invention.

[0062] Figure 3 This is a statistical diagram of the effect of different soaking times on the polysaccharide extraction rate of the present invention.

[0063] Figure 4 This is a statistical diagram of the effect of different ultrasonic treatment times on the polysaccharide extraction rate of the present invention.

[0064] Figure 5 This is a response surface orthogonal experiment result diagram of the material-liquid ratio and ultrasonic time of the present invention.

[0065] Figure 6 This is a response surface orthogonal experimental result diagram of the ultrasonic time and immersion time of the present invention.

[0066] Figure 7 It is a response surface orthogonal experiment result diagram of the material-liquid ratio and soaking time of the present invention.

[0067] Figure 8 This is a statistical chart showing the effects of different polysaccharides on DPPH radical scavenging rate.

[0068] Fig. 9 This is a statistical chart showing the effects of different polysaccharides on the scavenging rate of hydroxyl radicals.

[0069] Fig.10 This is a statistical chart showing the effects of different polysaccharides on superoxide anion clearance rate.

[0070] Fig.11 Comparison of weighted swimming time among mice in different groups.

[0071] Fig.12 Are the measurement results of biochemical indicators of mice, where K is the blank group, E is the Erjingfang polysaccharide intervention group, H is the Polygonatum sibiricum polysaccharide intervention group, G is the Lycium barbarum polysaccharide intervention group, and Vc is the positive control group. DETAILED DESCRIPTION

[0072] The following will be combined with the embodiments to clearly and completely describe the concept of the present invention and the technical effects produced, so as to fully understand the purpose, characteristics and effects of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, other embodiments obtained by those skilled in the art without creative work are all within the scope of protection of the present invention.

[0073] In the description of the present invention, the description with reference to the terms "one embodiment", "some embodiments", "illustrative embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0074] In the embodiment of the present invention, the wolfberry powder is Ningxia wolfberry dry powder, and the polygonatum powder is Yunnan polygonatum dry powder, and the sieve mesh number is 60 mesh.

[0075] If the specific conditions are not specified in the examples, the experiments were carried out under conventional conditions or conditions recommended by the manufacturer. If the manufacturers of the reagents or instruments are not specified, they are all conventional products that can be purchased commercially.

[0076] Example 1

[0077] This embodiment is a method for extracting polysaccharides from Erjingfang, which specifically includes the following steps:

[0078] Step S1, taking 10g of wolfberry and polygonatum powder, mixing them well and adding them into a Soxhlet extractor, then adding 10 times the amount of anhydrous ethanol and refluxing for 1h for degreasing, then collecting the filter residue and drying it in a 60°C oven to obtain defatted medicinal material powder;

[0079] Step S2, taking the above-mentioned defatted medicinal powder, adding water to the defatted medicinal powder at a solid-liquid ratio of 1g:10mL, soaking it at 60°C for 60min, and then ultrasonically treating it at an ultrasonic power of 600W for 35min. After the ultrasonic treatment, centrifuge it at 5000rpm for 10min, filter it, collect the filtrate, concentrate it 10 times on a rotary evaporator, collect the concentrate, and then precipitate it with 90% (final concentration) ethanol for 12h, collect the precipitate, and dry it in an oven at 60°C to obtain Erjingfang polysaccharide.

[0080] Example 2

[0081] This embodiment is a method for extracting polysaccharides from Erjingfang, which differs from the above-mentioned embodiment 1 in that the material-liquid ratio in step S2 is 1 g:15 mL, and the other conditions remain unchanged.

[0082] Example 3

[0083] This embodiment is a method for extracting polysaccharides from Erjingfang, which differs from the above-mentioned embodiment 1 in that the material-liquid ratio in step S2 is 1 g:20 mL, and the other conditions remain unchanged.

[0084] Example 4

[0085] This embodiment is a method for extracting polysaccharides from Erjingfang, which differs from the above-mentioned embodiment 1 in that the material-liquid ratio in step S2 is 1 g:25 mL, and the other conditions remain unchanged.

[0086] Example 5

[0087] This embodiment is a method for extracting polysaccharides from Erjingfang, which is different from the above-mentioned embodiment 1 in that the material-liquid ratio in step S2 is 1 g:30 mL, and the other conditions remain unchanged.

[0088] Example 6

[0089] This embodiment is a method for extracting polysaccharides from Erjingfang, which specifically includes the following steps:

[0090] Step S1, taking 10g of wolfberry and polygonatum powder, mixing them well and adding them into a Soxhlet extractor, then adding 10 times the amount of anhydrous ethanol and refluxing for 1h for degreasing, then collecting the filter residue and drying it in a 60°C oven to obtain defatted medicinal material powder;

[0091] Step S2, taking the above-mentioned defatted medicinal powder, adding water to the defatted medicinal powder at a solid-liquid ratio of 1g:10mL, soaking it at 60°C for 30min, and then ultrasonically treating it at an ultrasonic power of 600W for 35min. After the ultrasonic treatment, centrifuge it at 5000rpm for 10min, filter it, collect the filtrate, concentrate it 10 times on a rotary evaporator, collect the concentrate, and then precipitate it with 90% (final concentration) ethanol for 12h, collect the precipitate, and dry it in an oven at 60°C to obtain Erjingfang polysaccharide.

[0092] Example 7

[0093] This embodiment is a method for extracting Erjingfang polysaccharide, which is different from the above-mentioned embodiment 6 in that the soaking time in step S2 is 60 minutes, and the other conditions remain unchanged.

[0094] Example 8

[0095] This embodiment is a method for extracting Erjingfang polysaccharide, which is different from the above-mentioned embodiment 6 in that the soaking treatment time in step S2 is 90 minutes, and the other conditions remain unchanged.

[0096] Example 9

[0097] This embodiment is a method for extracting Erjingfang polysaccharide, which is different from the above-mentioned embodiment 6 in that the soaking treatment time in step S2 is 120 minutes, and the other conditions remain unchanged.

[0098] Example 10

[0099] This embodiment is a method for extracting Erjingfang polysaccharide, which is different from the above-mentioned embodiment 6 in that the soaking treatment time in step S2 is 150 minutes, and the other conditions remain unchanged.

[0100] Example 11 (ultrasonic treatment time)

[0101] This embodiment is a method for extracting polysaccharides from Erjingfang, which specifically includes the following steps:

[0102] Step S1, taking 10g of wolfberry and polygonatum powder, mixing them well and adding them into a Soxhlet extractor, then adding 10 times the amount of anhydrous ethanol and refluxing for 1h for degreasing, then collecting the filter residue and drying it in a 60°C oven to obtain defatted medicinal material powder;

[0103] Step S2, taking the above-mentioned defatted medicinal powder, adding water to the defatted medicinal powder at a solid-liquid ratio of 1g:10mL, soaking it at 60°C for 30min, and then ultrasonically treating it at an ultrasonic power of 600W for 5min. After the ultrasonic treatment, centrifuge it at 5000rpm for 10min, filter it, collect the filtrate, concentrate it 10 times by rotary evaporator, collect the concentrate, and then precipitate it with 90% (final concentration) ethanol for 12h, collect the precipitate, and dry it in an oven at 60°C to obtain Erjingfang polysaccharide.

[0104] Example 12

[0105] This embodiment is a method for extracting polysaccharides from Erjingfang, which differs from the above-mentioned embodiment 11 in that the ultrasonic treatment time in step S2 is 20 minutes, and the other conditions remain unchanged.

[0106] Embodiment 13

[0107] This embodiment is a method for extracting polysaccharides from Erjingfang, which differs from the above-mentioned embodiment 11 in that the ultrasonic treatment time in step S2 is 35 minutes, and the other conditions remain unchanged.

[0108] Embodiment 14

[0109] This embodiment is a method for extracting polysaccharides from Erjingfang, which differs from the above-mentioned embodiment 11 in that the ultrasonic treatment time in step S2 is 50 minutes, and the other conditions remain unchanged.

[0110] Embodiment 15

[0111] This embodiment is a method for extracting Erjingfang polysaccharide, which differs from the above-mentioned embodiment 11 in that the ultrasonic treatment time in step S2 is 65 minutes, and the other conditions remain unchanged.

[0112] Example 16

[0113] This embodiment is a method for extracting polysaccharides from Erjingfang, which specifically includes the following steps:

[0114] Step S1, taking 10g of wolfberry and polygonatum powder, mixing them well and adding them into a Soxhlet extractor, then adding 10 times the amount of anhydrous ethanol and refluxing for 1h for degreasing, then collecting the filter residue and drying it in a 60°C oven to obtain defatted medicinal material powder;

[0115] Step S2, taking the above-mentioned defatted medicinal material powder, adding water to the defatted medicinal material powder at a solid-liquid ratio of 1g:10mL, soaking for 30min at 60°C, and then ultrasonically treating for 35min at an ultrasonic power of 600W, centrifuging at 5000rpm for 10min after the ultrasonic treatment, filtering, collecting the filtrate, concentrating 10 times by rotary evaporator, collecting the concentrated solution, and then precipitating with 90% (final concentration) ethanol for 12h, collecting the precipitate, and drying in a 60°C oven to obtain di-fine square crude polysaccharide;

[0116] Step S3, adding distilled water to the above-mentioned crude polysaccharide to prepare a crude polysaccharide solution with a concentration of 1 mg / ml, then adding activated carbon with a final concentration of 0.010 g / mL, decolorizing at 50°C for 30 minutes, filtering to remove the activated carbon, and obtaining the polysaccharide.

[0117] Embodiment 17

[0118] This embodiment is a method for extracting polysaccharides from Erjingfang, which differs from the above-mentioned embodiment 16 in that the final concentration of activated carbon in step S3 is 0.015 g / mL, and the other conditions remain unchanged.

[0119] Embodiment 18

[0120] This embodiment is a method for extracting polysaccharides from Erjingfang, which differs from the above-mentioned embodiment 16 in that the final concentration of activated carbon in step S3 is 0.020 g / mL, and the other conditions remain unchanged.

[0121] Embodiment 19

[0122] This embodiment is a method for extracting polysaccharides from Erjingfang, which differs from the above-mentioned embodiment 16 in that the final concentration of activated carbon in step S3 is 0.030 g / mL, and the other conditions remain unchanged.

[0123] Embodiment 20

[0124] This embodiment is a method for extracting polysaccharides from Erjingfang, which differs from the above-mentioned embodiment 16 in that the final concentration of activated carbon in step S3 is 0.035 g / mL, and the other conditions remain unchanged.

[0125] Embodiment 21

[0126] This embodiment is a method for extracting polysaccharides from Erjingfang, which specifically includes the following steps:

[0127] Step S1, taking 10g of wolfberry and polygonatum powder, mixing them well and adding them into a Soxhlet extractor, then adding 10 times the amount of anhydrous ethanol and refluxing for 1h for degreasing, then collecting the filter residue and drying it in a 60°C oven to obtain defatted medicinal material powder;

[0128] Step S2, taking the above-mentioned defatted medicinal material powder, adding water to the defatted medicinal material powder at a solid-liquid ratio of 1g:10mL, soaking for 30min at 60°C, and then ultrasonically treating for 35min at an ultrasonic power of 600W, centrifuging at 5000rpm for 10min after the ultrasonic treatment, filtering, collecting the filtrate, concentrating 10 times by rotary evaporator, collecting the concentrated solution, and then precipitating with 90% (final concentration) ethanol for 12h, collecting the precipitate, and drying in a 60°C oven to obtain di-fine square crude polysaccharide;

[0129] Step S3, adding distilled water to the above-mentioned crude polysaccharide to prepare a crude polysaccharide solution with a concentration of 1 mg / ml, then adding activated carbon with a final concentration of 0.015 g / mL, decolorizing at 50°C for 10 minutes, filtering to remove the activated carbon, and obtaining the polysaccharide.

[0130] Embodiment 22

[0131] This embodiment is a method for extracting Erjingfang polysaccharide, which differs from the above-mentioned embodiment 21 in that the decolorization treatment time in step S3 is 20 minutes, and the other conditions remain unchanged.

[0132] Embodiment 23

[0133] This embodiment is a method for extracting Erjingfang polysaccharide, which differs from the above-mentioned embodiment 21 in that the decolorization treatment time in step S3 is 30 minutes, and the other conditions remain unchanged.

[0134] Embodiment 24

[0135] This embodiment is a method for extracting Erjingfang polysaccharide, which differs from the above-mentioned embodiment 21 in that the decolorization treatment time in step S3 is 40 minutes, and the other conditions remain unchanged.

[0136] Embodiment 25

[0137] This embodiment is a method for extracting Erjingfang polysaccharide, which differs from the above-mentioned embodiment 21 in that the decolorization treatment time in step S3 is 50 minutes, and the other conditions remain unchanged.

[0138] Embodiment 26

[0139] This embodiment is a method for extracting Erjingfang polysaccharide, which differs from the above-mentioned embodiment 21 in that the decolorization treatment time in step S3 is 60 minutes, and the other conditions remain unchanged.

[0140] Embodiment 27

[0141] This embodiment is a method for extracting polysaccharides from Erjingfang, which specifically includes the following steps:

[0142] Step S1, taking 10g of wolfberry and polygonatum powder, mixing them well and adding them into a Soxhlet extractor, then adding 10 times the amount of anhydrous ethanol and refluxing for 1h for degreasing, then collecting the filter residue and drying it in a 60°C oven to obtain defatted medicinal material powder;

[0143] Step S2, taking the above-mentioned defatted medicinal material powder, adding water to the defatted medicinal material powder at a solid-liquid ratio of 1g:10mL, soaking for 30min at 60°C, and then ultrasonically treating for 35min at an ultrasonic power of 600W, centrifuging at 5000rpm for 10min after the ultrasonic treatment, filtering, collecting the filtrate, concentrating 10 times by rotary evaporator, collecting the concentrated solution, and then precipitating with 90% (final concentration) ethanol for 12h, collecting the precipitate, and drying in a 60°C oven to obtain di-fine square crude polysaccharide;

[0144] Step S3, adding distilled water to the above-mentioned crude polysaccharide to prepare a crude polysaccharide solution with a concentration of 1 mg / ml, and then adding activated carbon with a final concentration of 0.015 g / mL, decolorizing at 30°C for 30 minutes, filtering to remove the activated carbon, and obtaining the polysaccharide.

[0145] Embodiment 28

[0146] This embodiment is a method for extracting Erjingfang polysaccharide, which differs from the above-mentioned embodiment 27 in that the temperature of the decolorization treatment in step S3 is 40°C, and the other conditions remain unchanged.

[0147] Embodiment 29

[0148] This embodiment is a method for extracting Erjingfang polysaccharide, which differs from the above-mentioned embodiment 27 in that the temperature of the decolorization treatment in step S3 is 50°C, and the other conditions remain unchanged.

[0149] Embodiment 30

[0150] This embodiment is a method for extracting Erjingfang polysaccharide, which differs from the above-mentioned embodiment 27 in that the temperature of the decolorization treatment in step S3 is 60°C, and the other conditions remain unchanged.

[0151] Embodiment 31

[0152] This embodiment is a method for extracting Erjingfang polysaccharide, which differs from the above-mentioned embodiment 27 in that the temperature of the decolorization treatment in step S3 is 70°C, and the other conditions remain unchanged.

[0153] Embodiment 32

[0154] This embodiment is a method for extracting Erjingfang polysaccharide, which differs from the above-mentioned embodiment 27 in that the temperature of the decolorization treatment in step S3 is 80°C, and the other conditions remain unchanged.

[0155] Embodiment 33

[0156] This embodiment is a method for extracting polysaccharides from Erjingfang, which specifically includes the following steps:

[0157] Step S1, taking 10g of wolfberry and polygonatum powder, mixing them well and adding them into a Soxhlet extractor, then adding 10 times the amount of anhydrous ethanol and refluxing for 1h for degreasing, then collecting the filter residue and drying it in a 60°C oven to obtain defatted medicinal material powder;

[0158] Step S2, taking the above-mentioned defatted medicinal powder, adding water to the defatted medicinal powder at a solid-liquid ratio of 1g:15mL, soaking it at 60°C for 40min, and then ultrasonically treating it at an ultrasonic power of 600W for 45min. After the ultrasonic treatment, centrifuge it at 5000rpm for 10min, filter it, collect the filtrate, concentrate it 10 times by rotary evaporator, collect the concentrate, and then precipitate it with 90% (final concentration) ethanol for 12h, collect the precipitate, and dry it in an oven at 60°C to obtain Erjingfang polysaccharide.

[0159] Embodiment 34

[0160] This embodiment is a method for extracting polysaccharides from Erjingfang, which specifically includes the following steps:

[0161] Step S1, taking 10g of wolfberry and polygonatum powder, mixing them well and adding them into a Soxhlet extractor, then adding 10 times the amount of anhydrous ethanol and refluxing for 1h for degreasing, then collecting the filter residue and drying it in a 60°C oven to obtain defatted medicinal material powder;

[0162] Step S2, taking the above-mentioned defatted medicinal powder, adding water to the defatted medicinal powder at a solid-liquid ratio of 1g:15mL, soaking it at 60°C for 40min, and then ultrasonically treating it at an ultrasonic power of 600W for 25min. After the ultrasonic treatment, centrifuge it at 5000rpm for 10min, filter it, collect the filtrate, concentrate it 10 times by rotary evaporator, collect the concentrate, and then precipitate it with 90% (final concentration) ethanol for 12h, collect the precipitate, and dry it in an oven at 60°C to obtain Erjingfang polysaccharide.

[0163] Embodiment 35

[0164] This embodiment is a method for extracting polysaccharides from Erjingfang, which specifically includes the following steps:

[0165] Step S1, taking 10g of wolfberry and polygonatum powder, mixing them well and adding them into a Soxhlet extractor, then adding 10 times the amount of anhydrous ethanol and refluxing for 1h for degreasing, then collecting the filter residue and drying it in a 60°C oven to obtain defatted medicinal material powder;

[0166] Step S2, taking the above-mentioned defatted medicinal powder, adding water to the defatted medicinal powder at a solid-liquid ratio of 1g:10mL, soaking it at 60°C for 20min, and then ultrasonically treating it at an ultrasonic power of 600W for 35min. After the ultrasonic treatment, centrifuge it at 5000rpm for 10min, filter it, collect the filtrate, concentrate it 10 times by rotary evaporator, collect the concentrate, and then precipitate it with 90% (final concentration) ethanol for 12h, collect the precipitate, and dry it in an oven at 60°C to obtain Erjingfang polysaccharide.

[0167] Embodiment 36

[0168] This embodiment is a method for extracting polysaccharides from Erjingfang, which specifically includes the following steps:

[0169] Step S1, taking 10g of wolfberry and polygonatum powder, mixing them well and adding them into a Soxhlet extractor, then adding 10 times the amount of anhydrous ethanol and refluxing for 1h for degreasing, then collecting the filter residue and drying it in a 60°C oven to obtain defatted medicinal material powder;

[0170] Step S2, taking the above-mentioned defatted medicinal powder, adding water to the defatted medicinal powder at a solid-liquid ratio of 1g:20mL, soaking it at 60°C for 30min, and then ultrasonically treating it at an ultrasonic power of 600W for 45min. After the ultrasonic treatment, centrifuge it at 5000rpm for 10min, filter it, collect the filtrate, concentrate it 10 times by rotary evaporator, collect the concentrate, and then precipitate it with 90% (final concentration) ethanol for 12h, collect the precipitate, and dry it in an oven at 60°C to obtain Erjingfang polysaccharide.

[0171] Embodiment 37

[0172] This embodiment is a method for extracting polysaccharides from Erjingfang, which specifically includes the following steps:

[0173] Step S1, taking 10g of wolfberry and polygonatum powder, mixing them well and adding them into a Soxhlet extractor, then adding 10 times the amount of anhydrous ethanol and refluxing for 1h for degreasing, then collecting the filter residue and drying it in a 60°C oven to obtain defatted medicinal material powder;

[0174] Step S2, taking the above-mentioned defatted medicinal powder, adding water to the defatted medicinal powder at a solid-liquid ratio of 1g:15mL, soaking it at 60°C for 20min, and then ultrasonically treating it at an ultrasonic power of 600W for 45min. After the ultrasonic treatment, centrifuge it at 5000rpm for 10min, filter it, collect the filtrate, concentrate it 10 times by rotary evaporator, collect the concentrate, and then precipitate it with 90% (final concentration) ethanol for 12h, collect the precipitate, and dry it in an oven at 60°C to obtain Erjingfang polysaccharide.

[0175] Embodiment 38

[0176] This embodiment is a method for extracting polysaccharides from Erjingfang, which specifically includes the following steps:

[0177] Step S1, taking 10g of wolfberry and polygonatum powder, mixing them well and adding them into a Soxhlet extractor, then adding 10 times the amount of anhydrous ethanol and refluxing for 1h for degreasing, then collecting the filter residue and drying it in a 60°C oven to obtain defatted medicinal material powder;

[0178] Step S2, taking the above-mentioned defatted medicinal powder, adding water to the defatted medicinal powder at a solid-liquid ratio of 1g:15mL, soaking it at 60°C for 30min, and then ultrasonically treating it at an ultrasonic power of 600W for 35min. After the ultrasonic treatment, centrifuge it at 5000rpm for 10min, filter it, collect the filtrate, concentrate it 10 times by rotary evaporator, collect the concentrate, and then precipitate it with 90% (final concentration) ethanol for 12h, collect the precipitate, and dry it in an oven at 60°C to obtain Erjingfang polysaccharide.

[0179] Embodiment 39

[0180] This embodiment is a method for extracting polysaccharides from Erjingfang, which specifically includes the following steps:

[0181] Step S1, taking 10g of wolfberry and polygonatum powder, mixing them well and adding them into a Soxhlet extractor, then adding 10 times the amount of anhydrous ethanol and refluxing for 1h for degreasing, then collecting the filter residue and drying it in a 60°C oven to obtain defatted medicinal material powder;

[0182] Step S2, taking the above-mentioned defatted medicinal powder, adding water to the defatted medicinal powder at a solid-liquid ratio of 1g:10mL, soaking it at 60°C for 30min, and then ultrasonically treating it at an ultrasonic power of 600W for 25min. After the ultrasonic treatment, centrifuge it at 5000rpm for 10min, filter it, collect the filtrate, concentrate it 10 times by rotary evaporator, collect the concentrate, and then precipitate it with 90% (final concentration) ethanol for 12h, collect the precipitate, and dry it in an oven at 60°C to obtain Erjingfang polysaccharide.

[0183] Embodiment 40

[0184] This embodiment is a method for extracting polysaccharides from Erjingfang, which specifically includes the following steps:

[0185] Step S1, taking 10g of wolfberry and polygonatum powder, mixing them well and adding them into a Soxhlet extractor, then adding 10 times the amount of anhydrous ethanol and refluxing for 1h for degreasing, then collecting the filter residue and drying it in a 60°C oven to obtain defatted medicinal material powder;

[0186] Step S2, taking the above-mentioned defatted medicinal powder, adding water to the defatted medicinal powder at a solid-liquid ratio of 1g:20mL, soaking it at 60°C for 20min, and then ultrasonically treating it at an ultrasonic power of 600W for 35min. After the ultrasonic treatment, centrifuge it at 5000rpm for 10min, filter it, collect the filtrate, concentrate it 10 times by rotary evaporator, collect the concentrate, and then precipitate it with 90% (final concentration) ethanol for 12h, collect the precipitate, and dry it in an oven at 60°C to obtain Erjingfang polysaccharide.

[0187] Embodiment 41

[0188] This embodiment is a method for extracting polysaccharides from Erjingfang, which specifically includes the following steps:

[0189] Step S1, taking 10g of wolfberry and polygonatum powder, mixing them well and adding them into a Soxhlet extractor, then adding 10 times the amount of anhydrous ethanol and refluxing for 1h for degreasing, then collecting the filter residue and drying it in a 60°C oven to obtain defatted medicinal material powder;

[0190] Step S2, taking the above-mentioned defatted medicinal powder, adding water to the defatted medicinal powder at a solid-liquid ratio of 1g:15mL, soaking it at 60°C for 30min, and then ultrasonically treating it at an ultrasonic power of 600W for 35min. After the ultrasonic treatment, centrifuge it at 5000rpm for 10min, filter it, collect the filtrate, concentrate it 10 times by rotary evaporator, collect the concentrate, and then precipitate it with 90% (final concentration) ethanol for 12h, collect the precipitate, and dry it in an oven at 60°C to obtain Erjingfang polysaccharide.

[0191] Embodiment 42

[0192] This embodiment is a method for extracting polysaccharides from Erjingfang, which specifically includes the following steps:

[0193] Step S1, taking 10g of wolfberry and polygonatum powder, mixing them well and adding them into a Soxhlet extractor, then adding 10 times the amount of anhydrous ethanol and refluxing for 1h for degreasing, then collecting the filter residue and drying it in a 60°C oven to obtain defatted medicinal material powder;

[0194] Step S2, taking the above-mentioned defatted medicinal powder, adding water to the defatted medicinal powder at a solid-liquid ratio of 1g:10mL, soaking it at 60°C for 30min, and then ultrasonically treating it at an ultrasonic power of 600W for 45min. After the ultrasonic treatment, centrifuge it at 5000rpm for 10min, filter it, collect the filtrate, concentrate it 10 times by rotary evaporator, collect the concentrate, and then precipitate it with 90% (final concentration) ethanol for 12h, collect the precipitate, and dry it in an oven at 60°C to obtain Erjingfang polysaccharide.

[0195] Embodiment 43

[0196] This embodiment is a method for extracting polysaccharides from Erjingfang, which specifically includes the following steps:

[0197] Step S1, taking 10g of wolfberry and polygonatum powder, mixing them well and adding them into a Soxhlet extractor, then adding 10 times the amount of anhydrous ethanol and refluxing for 1h for degreasing, then collecting the filter residue and drying it in a 60°C oven to obtain defatted medicinal material powder;

[0198] Step S2, taking the above-mentioned defatted medicinal powder, adding water to the defatted medicinal powder at a solid-liquid ratio of 1g:15mL, soaking it at 60°C for 30min, and then ultrasonically treating it at an ultrasonic power of 600W for 35min. After the ultrasonic treatment, centrifuge it at 5000rpm for 10min, filter it, collect the filtrate, concentrate it 10 times by rotary evaporator, collect the concentrate, and then precipitate it with 90% (final concentration) ethanol for 12h, collect the precipitate, and dry it in an oven at 60°C to obtain Erjingfang polysaccharide.

[0199] Embodiment 44

[0200] This embodiment is a method for extracting polysaccharides from Erjingfang, which specifically includes the following steps:

[0201] Step S1, taking 10g of wolfberry and polygonatum powder, mixing them well and adding them into a Soxhlet extractor, then adding 10 times the amount of anhydrous ethanol and refluxing for 1h for degreasing, then collecting the filter residue and drying it in a 60°C oven to obtain defatted medicinal material powder;

[0202] Step S2, taking the above-mentioned defatted medicinal powder, adding water to the defatted medicinal powder at a solid-liquid ratio of 1g:20mL, soaking it at 60°C for 40min, and then ultrasonically treating it at an ultrasonic power of 600W for 35min. After the ultrasonic treatment, centrifuge it at 5000rpm for 10min, filter it, collect the filtrate, concentrate it 10 times on a rotary evaporator, collect the concentrate, and then precipitate it with 90% (final concentration) ethanol for 12h, collect the precipitate, and dry it in an oven at 60°C to obtain Erjingfang polysaccharide.

[0203] Embodiment 45

[0204] This embodiment is a method for extracting polysaccharides from Erjingfang, which specifically includes the following steps:

[0205] Step S1, taking 10g of wolfberry and polygonatum powder, mixing them well and adding them into a Soxhlet extractor, then adding 10 times the amount of anhydrous ethanol and refluxing for 1h for degreasing, then collecting the filter residue and drying it in a 60°C oven to obtain defatted medicinal material powder;

[0206] Step S2, taking the above-mentioned defatted medicinal powder, adding water to the defatted medicinal powder at a solid-liquid ratio of 1g:15mL, soaking it at 60°C for 30min, and then ultrasonically treating it at an ultrasonic power of 600W for 35min. After the ultrasonic treatment, centrifuge it at 5000rpm for 10min, filter it, collect the filtrate, concentrate it 10 times by rotary evaporator, collect the concentrate, and then precipitate it with 90% (final concentration) ethanol for 12h, collect the precipitate, and dry it in an oven at 60°C to obtain Erjingfang polysaccharide.

[0207] Embodiment 46

[0208] This embodiment is a method for extracting polysaccharides from Erjingfang, which specifically includes the following steps:

[0209] Step S1, taking 10g of wolfberry and polygonatum powder, mixing them well and adding them into a Soxhlet extractor, then adding 10 times the amount of anhydrous ethanol and refluxing for 1h for degreasing, then collecting the filter residue and drying it in a 60°C oven to obtain defatted medicinal material powder;

[0210] Step S2, taking the above-mentioned defatted medicinal powder, adding water to the defatted medicinal powder at a solid-liquid ratio of 1g:15mL, soaking it at 60°C for 20min, and then ultrasonically treating it at an ultrasonic power of 600W for 25min. After the ultrasonic treatment, centrifuge it at 5000rpm for 10min, filter it, collect the filtrate, concentrate it 10 times by rotary evaporator, collect the concentrate, and then precipitate it with 90% (final concentration) ethanol for 12h, collect the precipitate, and dry it in an oven at 60°C to obtain Erjingfang polysaccharide.

[0211] Embodiment 47

[0212] This embodiment is a method for extracting polysaccharides from Erjingfang, which specifically includes the following steps:

[0213] Step S1, taking 10g of wolfberry and polygonatum powder, mixing them well and adding them into a Soxhlet extractor, then adding 10 times the amount of anhydrous ethanol and refluxing for 1h for degreasing, then collecting the filter residue and drying it in a 60°C oven to obtain defatted medicinal material powder;

[0214] Step S2, taking the above-mentioned defatted medicinal powder, adding water to the defatted medicinal powder at a solid-liquid ratio of 1g:15mL, soaking it at 60°C for 30min, and then ultrasonically treating it at an ultrasonic power of 600W for 35min. After the ultrasonic treatment, centrifuge it at 5000rpm for 10min, filter it, collect the filtrate, concentrate it 10 times by rotary evaporator, collect the concentrate, and then precipitate it with 90% (final concentration) ethanol for 12h, collect the precipitate, and dry it in an oven at 60°C to obtain Erjingfang polysaccharide.

[0215] Embodiment 48

[0216] This embodiment is a method for extracting polysaccharides from Erjingfang, which specifically includes the following steps:

[0217] Step S1, taking 10g of wolfberry and polygonatum powder, mixing them well and adding them into a Soxhlet extractor, then adding 10 times the amount of anhydrous ethanol and refluxing for 1h for degreasing, then collecting the filter residue and drying it in a 60°C oven to obtain defatted medicinal material powder;

[0218] Step S2, taking the above-mentioned defatted medicinal powder, adding water to the defatted medicinal powder at a solid-liquid ratio of 1g:10mL, soaking it at 60°C for 40min, and then ultrasonically treating it at an ultrasonic power of 600W for 35min. After the ultrasonic treatment, centrifuge it at 5000rpm for 10min, filter it, collect the filtrate, concentrate it 10 times by rotary evaporator, collect the concentrate, and then precipitate it with 90% (final concentration) ethanol for 12h, collect the precipitate, and dry it in an oven at 60°C to obtain Erjingfang polysaccharide.

[0219] Embodiment 49

[0220] This embodiment is a method for extracting polysaccharides from Erjingfang, which specifically includes the following steps:

[0221] Step S1, taking 10g of wolfberry and polygonatum powder, mixing them well and adding them into a Soxhlet extractor, then adding 10 times the amount of anhydrous ethanol and refluxing for 1h for degreasing, then collecting the filter residue and drying it in a 60°C oven to obtain defatted medicinal material powder;

[0222] Step S2, taking the above-mentioned defatted medicinal powder, adding water to the defatted medicinal powder at a solid-liquid ratio of 1g:20mL, soaking it at 60°C for 30min, and then ultrasonically treating it at an ultrasonic power of 600W for 25min. After the ultrasonic treatment, centrifuge it at 5000rpm for 10min, filter it, collect the filtrate, concentrate it 10 times on a rotary evaporator, collect the concentrate, and then precipitate it with 90% (final concentration) ethanol for 12h, collect the precipitate, and dry it in an oven at 60°C to obtain Erjingfang polysaccharide.

[0223] Comparative Example 1

[0224] This comparative example is a method for extracting Erjingfang polysaccharide. Compared with Example 13, the difference between this comparative example and Example 13 is that the wolfberry and polygonatum powder in step S1 are not defatted, and the other conditions remain unchanged.

[0225] Comparative Example 2

[0226] This comparative example is a method for extracting Erjingfang polysaccharide, which specifically comprises the following steps:

[0227] Step S1, taking 10g of wolfberry and polygonatum powder, mixing them well and adding them into a Soxhlet extractor, then adding 10 times the amount of anhydrous ethanol and refluxing for 1h for degreasing, then collecting the filter residue and drying it in a 60°C oven to obtain defatted medicinal material powder;

[0228] Step S2, taking the above-mentioned defatted medicinal powder, adding water to the defatted medicinal powder at a solid-liquid ratio of 1g:10mL, soaking at 60°C for 30min, filtering, adding 90% (final concentration) ethanol to the combined filtrate for 12h, collecting the precipitate, and placing it in a 60°C oven to dry, thereby obtaining Erjingfang polysaccharide.

[0229] Comparative Example 3

[0230] This comparative example is a method for extracting Erjingfang polysaccharide, which specifically comprises the following steps:

[0231] Step S1, taking 10g of wolfberry and polygonatum powder, mixing them well and adding them into a Soxhlet extractor, then adding 10 times the amount of anhydrous ethanol and refluxing for 1h for degreasing, then collecting the filter residue and drying it in a 60°C oven to obtain defatted medicinal material powder;

[0232] Step S2, taking the above-mentioned defatted medicinal powder, adding water to the defatted medicinal powder at a solid-liquid ratio of 1g:10mL, ultrasonically treating for 35min at an ultrasonic power of 600W, centrifuging at 5000rpm for 10min after the ultrasonic treatment, filtering, collecting the filtrate, concentrating 10 times by rotary evaporator, collecting the concentrated solution, and then precipitating with 90% (final concentration) ethanol for 12h, collecting the precipitate, and drying in an oven at 60°C to obtain Erjingfang polysaccharide.

[0233] Comparative Example 4

[0234] This comparative example is a method for extracting polygonatum polysaccharide, which differs from Example 38 in that wolfberry is not added in step S1, and other conditions remain unchanged.

[0235] Comparative Example 5

[0236] This comparative example is a method for extracting wolfberry polysaccharides, which differs from Example 38 in that no polygonatum powder is added in step S1, and other conditions remain unchanged.

[0237] In order to facilitate a better understanding of the present invention, the solid-liquid ratio, immersion time, ultrasonic treatment time, activated carbon dosage, activated carbon decolorization time and activated carbon decolorization temperature of the above embodiments are statistically analyzed, as shown in Table 1.

[0238] Table 1: Statistics of relevant parameters of Examples 1 to 49

[0239]

[0240]

[0241] (I) Single factor extraction condition optimization test

[0242] 1. Test methods

[0243] Take 20g of defatted dry powder, soak it at 60℃, take the solid-liquid ratio (1:10, 1:15, 1:20, 1:25 and 1:30) as variables, set the soaking time to 60min, the ultrasonic time to 35min, and the ultrasonic power to 600W for extraction. After the extract is concentrated, use 90% ethanol to precipitate, let it stand overnight, and weigh the precipitate after drying to obtain crude polysaccharides. Calculate the yield of Erjingfang polysaccharide and obtain the optimal solid-liquid ratio; then, according to the optimal solid-liquid ratio, take the soaking time (30min, 60min, 90min, 120min and 150min) as variables to obtain the optimal soaking time; finally, according to the optimal solid-liquid ratio and the optimal soaking time, take the ultrasonic time (5min, 20min, 35min, 50min and 65min) as variables to obtain the optimal ultrasonic time. The specific detection method of the extraction rate of Erjingfang polysaccharide is as follows:

[0244] (1) Preparation of glucose standard solution and drawing of standard curve: According to the phenol-sulfuric acid method, anhydrous glucose was used as the reference substance to determine the polysaccharide content. 10 mg of anhydrous glucose reference substance dried to constant weight at 105°C was taken. Weigh accurately, place in a 100mL volumetric flask, add water to dissolve and dilute to the scale, shake well to obtain (each 1mL contains 0.1mg of anhydrous glucose). Accurately measure 0.1mL, 0.2mL, 0.3mL, 0.4mL, 0.5mL, and 0.6mL of the reference solution, place in 10mL stoppered test tubes respectively, add water to make up to 2.0mL each, accurately add 1mL of 6% phenol solution (prepared before use), shake well, then accurately add 5mL of sulfuric acid, shake well, let it stand for 10min, keep warm in a 40℃ water bath for 15min, take out and quickly cool to room temperature, use the corresponding reagent as blank, measure the absorbance at a wavelength of 490nm by UV-visible spectrophotometry, and draw a standard curve with absorbance as the ordinate and concentration as the abscissa.

[0245] Draw the glucose standard curve according to the above method. Figure 1 As shown, the formula is: y=0.1033x-0.0052.

[0246] (2) Determination of the content of Erjinfang polysaccharide: 10 mg of each Erjinfang polysaccharide obtained in step S2 of the above Examples 1 to 15 was accurately weighed, dissolved in distilled water and fixed to volume in a 100 mL volumetric flask, and shaken well. 0.4 mL of Erjinfang polysaccharide solution was measured and placed in a 10 mL stoppered dry test tube. From "add water to 2.0 mL", the absorbance was measured according to the law, and the weight (mg) of anhydrous glucose in the test solution was calculated by the standard curve. The result was obtained by calculation. Three sets of replicates were made for each sample.

[0247] 2. Test results

[0248] (1) Effect of solid-liquid ratio on the extraction rate of polysaccharides from Erjingfang

[0249] The extraction rates of the polysaccharides in the two refined prescriptions of Examples 1 to 5 were tested, and the results are shown in Tables 2 and Figure 2 shown.

[0250] Table 2: Effect of solid-liquid ratio on the extraction rate of polysaccharides from Erjingfang

[0251] Material-liquid ratio / g / ml Polysaccharide yield / % Example 1 1:10 27.75％ Example 2 1:15 26.30％ Example 3 1:20 26.75％ Example 4 1:25 19.60％ Example 5 1:30 20.25％

[0252] (2) Effect of soaking time on the extraction rate of polysaccharides from Erjingfang

[0253] The extraction rates of the polysaccharides in the two refined prescriptions of Examples 6 to 10 were tested, and the results are shown in Tables 3 and Figure 3 shown.

[0254] Table 3: Effect of soaking time on the extraction rate of polysaccharides from Erjingfang

[0255] Soaking time / min Polysaccharide yield / % Example 6 30min 28.76％ Example 7 60min 23.30％ Example 8 90min 21.90％ Example 9 120min 20.20％ Example 10 150min 20.60％

[0256] (3) Effect of ultrasonic treatment time on the extraction rate of polysaccharides from Erjingfang

[0257] The extraction rates of the polysaccharides in the two refined prescriptions of Examples 11 to 15 were tested, and the results are shown in Tables 4 and Figure 4 shown.

[0258] Table 4: Effect of ultrasonic treatment time on the extraction rate of polysaccharides from Erjingfang

[0259] Ultrasonic time / min Polysaccharide yield / % Embodiment 11 5 20.15％ Example 12 10 21.30％ Embodiment 13 20 24.10％ Embodiment 14 35 28.90％ Embodiment 15 55 28.15％

[0260] The above test results show that: when the solid-liquid ratio is 1:10, the polysaccharide yield is the highest, reaching 27.75%; when the soaking time is 30min, the extraction rate is the highest, which is 28.76%, but with the increase of soaking time, the extraction rate gradually decreases, which may be more impurities are dissolved, thereby reducing the polysaccharide yield. The polysaccharide yield increases with the increase of ultrasonic time at the beginning, and reaches a maximum of 28.9% at 35min, and then decreases, which may also be due to the dissolution of impurities. Therefore, the optimal conditions for single-factor extraction are a solid-liquid ratio of 1:10, a soaking time of 30min, and an ultrasonic time of 35min.

[0261] (II) Response surface optimization experiment

[0262] According to the results of the above single factors, the Design Expert 10. Box-Behnken experimental design scheme was adopted, with the significantly influencing factors of immersion time (A), liquid-to-solid ratio (B) and ultrasonic time (C). The design factors and levels are shown in Table 5.

[0263] Table 5 Response surface experimental factors and level design

[0264]

[0265] Taking the polysaccharide yield as the response value, 17 groups of experiments with 3 factors and 3 levels (corresponding to Examples 33 to 49) were designed to conduct response surface experiments to optimize the polysaccharide extraction process of Erjingfang, obtain crude polysaccharides and measure the polysaccharide yield. The results are shown in Table 6. Figure 5 to Figure 7 shown.

[0266] Table 6: Response surface orthogonal experiment results

[0267]

[0268]

[0269] The above measurement results show that the optimal conditions screened by response surface methodology are as follows: the extraction solid-liquid ratio is 1:15, the soaking time is 30 minutes, the ultrasonic time is 35 minutes, the ultrasonic power is set to 600W, and the polysaccharide yield is 28.36%.

[0270] (III) Single factor experiment of activated carbon decolorization

[0271] The crude polysaccharide powder extracted under the above optimal conditions (Example 13) was subjected to an activated carbon decolorization single factor experiment. The three factors of activated carbon dosage, decolorization time and decolorization temperature were selected and single factor experiments were performed separately.

[0272] 1. Test methods

[0273] (1) Effect of activated carbon dosage on decolorization rate

[0274] Referring to the dosage range of activated carbon decolorization in industry, five activated carbon dosage levels of 0.0075, 0.01, 0.0125, 0.015, and 0.0175 g / mL were set (Examples 16 to 20). The decolorization was carried out at 40°C for 40 minutes, and the absorbance of the supernatant was measured after filtering and removing the carbon.

[0275] (2) Effect of decolorization time on decolorization rate

[0276] Six decolorization times of 10, 20, 30, 40, 50 and 60 min were set (Examples 21 to 26), the amount of activated carbon was 2 g / 100 mL, and then oscillation decolorization was carried out at the above-selected temperature. After filtering and removing the carbon, the absorbance value of the supernatant was measured.

[0277] (3) Effect of decolorization temperature on decolorization rate

[0278] Six decolorization temperatures of 30, 40, 50, 60, 70, and 80°C (Examples 27 to 32), the optimal amount of activated carbon and the decolorization time were set, and the absorbance of the supernatant was measured after filtering and removing the carbon to determine the optimal combination of the polysaccharide decolorization process.

[0279] 2. Test results

[0280] The test results of the effects of activated carbon addition amount, decolorization time and decolorization temperature on decolorization rate are shown in Tables 7 to 9.

[0281] Table 7: Effect of activated carbon addition on decolorization effect

[0282] Activated carbon content (g / ml) Decolorization rate Polysaccharide retention rate Example 16 0.0075 27.84％ 72.16％ Embodiment 17 0.01 25.84％ 74.16％ Embodiment 18 0.0125 33.84％ 66.16％ Embodiment 19 0.015 47.09％ 52.91％ Embodiment 20 0.0175 42.31％ 57.69％

[0283] Table 8: Effect of decolorization time on decolorization effect

[0284] Decolorization time (min) Decolorization rate Polysaccharide retention rate Embodiment 21 10 19.7％ 68.2％ Embodiment 22 20 22.49％ 73.02％ Embodiment 23 30 26.14％ 70.71％ Embodiment 24 40 24.60％ 86.55％ Embodiment 25 50 21.11％ 85.99％ Embodiment 26 60 11.73％ 69.56％

[0285] Table 9: Effect of decolorization temperature on decolorization rate

[0286] Decolorization temperature(℃) Decolorization rate Polysaccharide retention rate Embodiment 27 30 19.32％ 85.94％ Embodiment 28 40 26.92％ 68.83％ Embodiment 29 50 42.58％ 52.22％ Embodiment 30 60 16.42％ 80.45％ Embodiment 31 70 11.89％ 77.36％ Embodiment 32 80 11.20％ 68.2％

[0287] From the above test results, it can be seen that when the decolorization temperature is 50℃, the decolorization rate is the highest (42.58%), and the polysaccharide retention rate is 52.22%; the optimal decolorization time is 30min, and the optimal amount of activated carbon is 1.5g / 100ml. In summary, it can be seen that the optimal conditions are selected as 1.5g / 100ml activated carbon, 30min decolorization time, and 50℃ decolorization temperature, with a decolorization rate of 47.09% and a polysaccharide retention rate of 52.91%.

[0288] (IV) Experiment on the effect of different extraction methods on extraction rate

[0289] The above-mentioned polysaccharide extraction rate detection method was used to detect the extraction rates of the polysaccharide extraction methods of the Erjing Fang in Comparative Examples 1 to 3 of the present invention, with three replicates in each group. The detection results are shown in Table 10.

[0290] Table 10: Effect of different extraction methods on extraction rate

[0291] first(%) Second time (%) The third time (%) Average polysaccharide yield (%) Comparative Example 1 21.34 21.11 21.57 21.34 Comparative Example 2 19.53 18.36 20.2 19.36 Comparative Example 3 17.11 16.78 17.44 17.11

[0292] The above test results show that: compared with Example 13 of the present invention, under the same other conditions, the extraction rate of Erjingfang polysaccharide without defatting is 21.34%, which is significantly lower than the extraction rate of Erjingfang polysaccharide after defatting under the same conditions (28.90% in Example 13), which may be because the increase of lipid impurities is not conducive to polysaccharide extraction; the extraction rate of Erjingfang polysaccharide without ultrasonic treatment is 19.36%, and the extraction rate of Erjingfang polysaccharide without hot water immersion treatment is 17.11%, indicating that under the same conditions of other preparation methods, the extraction rate of Erjingfang polysaccharide can be significantly improved by defatting, hot water immersion and ultrasonic treatment.

[0293] Application Example 1: In vitro antioxidant activity experiment of Erjingfang polysaccharide

[0294] In this application example, the in vitro antioxidant activity experiment was conducted on the Erjingfang polysaccharide prepared in Example 38 of the present invention, and the antioxidant activity of Polygonatum sibiricum polysaccharide (Comparative Example 4) and Lycium barbarum polysaccharide (Comparative Example 5) was compared. By preparing Erjingfang polysaccharide, Polygonatum sibiricum polysaccharide and Lycium barbarum polysaccharide solutions with different concentration gradients, the removal of DPPH· free radicals, ·OH hydroxyl free radicals, O 2- Comparison of the ability of three groups of free radicals and their reducing power, and study of the relationship between their content and in vitro antioxidant activity.

[0295] 1. Experimental methods

[0296] (1) Detection of DPPH free radical scavenging ability: The Erjingfang polysaccharide prepared in Example 38 was prepared into Erjingfang polysaccharide solutions of different concentrations (0.5 mg / mL, 1.0 mg / mL, 2.0 mg / mL, 4.0 mg / mL, 6.0 mg / mL, 8.0 mg / mL and 10.0 mg / mL), and 2.0 mL of each solution was placed in different test tubes, 2.0 mL of 0.1 mmol / L DPPH solution was added, and the mixture was fully mixed and reacted in the dark for 30 min. The absorbance A1 was measured at 517 nm; 2.0 mL of the sample solution plus 2.0 ml of anhydrous ethanol was used as the blank control group, and its absorbance value measured at 527 nm was recorded as A3; 2.0 mL of DPPH solution plus 2.0 mL of distilled water was used as the model control group, and the absorbance measured at 517 nm was recorded as A2; the same method was used to replace the Erjingfang polysaccharide with a Vc solution of the same concentration gradient as the Erjingfang sample as a positive control. The same method was used to measure the DPPH free radical scavenging rate of Lycium barbarum polysaccharides and Polygonatum cyrtonema polysaccharides with the same concentration gradient as the Erjingfang sample. The scavenging rate of the sample on DPPH free radicals was calculated according to the following formula:

[0297] DPPH free radical scavenging rate (%) = [A2-(A1+A3)] / A2×100%.

[0298] (2) Hydroxyl radical scavenging ability test: The Erjingfang polysaccharide prepared in Example 38 was prepared into Erjingfang polysaccharide solutions of different concentrations (0.5 mg / mL, 1.0 mg / mL, 2.0 mg / mL, 4.0 mg / mL, 6.0 mg / mL, 8.0 mg / mL and 10.0 mg / mL), and 2.0 mL of each solution was placed in different test tubes. 2.0 mL of 6 mmol / L ferrous sulfate and 2.0 mL of 6 mmol / L hydrogen peroxide were added in sequence. After mixing, the mixture was allowed to stand for 10 min. 2.0 mL of 6 mmol / L salicylic acid-anhydrous ethanol were added respectively. After mixing, the mixture was allowed to stand for another 30 min. The absorbance was measured at 510 nm and recorded as A1. The absorbance was measured by replacing salicylic acid with anhydrous ethanol (other operations were the same) and recorded as A2. The polysaccharide solution was replaced with distilled water (other operations were the same) as a blank group, and the absorbance was measured as A3. The same method was used to replace Erjingfang polysaccharide with Vc solution of the same concentration gradient as the Erjingfang sample as a positive control. The same method was used to replace Erjingfang polysaccharide with Lycium barbarum polysaccharide and Polygonatum sibiricum polysaccharide of the same concentration gradient as the Erjingfang sample as a control to compare the hydroxyl radical scavenging ability of Erjingfang polysaccharide, Lycium barbarum polysaccharide and Polygonatum sibiricum polysaccharide. The scavenging rate of the sample on hydroxyl radicals was calculated according to the following formula:

[0299] Hydroxyl radical scavenging rate = (A3-A1+A2) / A3×100%.

[0300] (3) Superoxide anion scavenging ability test: The Erjinfang polysaccharide prepared in Example 38 was prepared into Erjinfang polysaccharide solutions of different concentrations (0.5 mg / mL, 1.0 mg / mL, 2.0 mg / mL, 4.0 mg / mL, 6.0 mg / mL, 8.0 mg / mL and 10.0 mg / mL), and then 4.5 mL of Tris-HCl (pH 8.2) buffer was taken, and after being incubated in a 25°C water bath for 20 min, 1.0 mL of the above-mentioned Erjinfang polysaccharide solutions of different concentrations were added, and then 0.4 mL of 25 mmol / L pyrogallol solution was added, and after being fully mixed, the mixture was reacted in a 25°C water bath for 5 min, and then 1 mL of The reaction was terminated with 8 mol / L hydrochloric acid, and the absorbance was measured at 299 nm and recorded as A1; the blank group used distilled water instead of polysaccharide solution, and the absorbance was measured and recorded as A2; the sample control group used distilled water instead of pyrogallol and hydrochloric acid, and the absorbance was measured as A3; the same method was used to replace the Erjingfang polysaccharide with a Vc solution with the same concentration gradient as the Erjingfang sample as a positive control. The same method was used to replace the Erjingfang polysaccharide with wolfberry and polygonatum polysaccharides with the same concentration gradient as the Erjingfang sample as a control, and the superoxide anion scavenging ability of Erjingfang polysaccharide, wolfberry polysaccharide and polygonatum polysaccharide was compared. The superanion scavenging rate of the sample was calculated according to the following formula:

[0301] Superoxide anion free radical scavenging rate = (A2-A1+A3) / A2×100%.

[0302] 2. Experimental results

[0303] The DPPH free radical, hydroxyl free radical and superoxide anion scavenging ability of the polysaccharide was determined according to the method of the above technical scheme. The results are as follows Figures 8 to 10 As shown, Figure 8 The results of DPPH free radical scavenging ability test. Fig. 9 The results of the hydroxyl radical scavenging ability test are as follows: Fig.10 This is the result of superoxide anion scavenging ability test.

[0304] Figure 8 The measurement results show that the DPPH radical scavenging rates of the Erjingfang polysaccharide prepared by the present invention and the polygonatum polysaccharide and wolfberry polysaccharide prepared in the comparative example increase with the increase of concentration, but the scavenging ability of the Erjingfang polysaccharide is significantly stronger than that of the polygonatum polysaccharide and wolfberry polysaccharide, which may be because the Erjingfang polysaccharide plays a certain synergistic role when the two are used in combination.

[0305] Fig. 9The measurement results show that the scavenging ability of the Erjingfang polysaccharide prepared by the present invention and the polygonatum polysaccharide and wolfberry polysaccharide prepared in the comparative example on hydroxyl radicals is proportional to the concentration, among which the scavenging ability of the Erjingfang polysaccharide on hydroxyl radicals is stronger than that of polygonatum polysaccharide and wolfberry polysaccharide, indicating that the synergistic effect of the polygonatum polysaccharide and wolfberry polysaccharide in the Erjingfang polysaccharide prepared by the present invention is obvious.

[0306] Fig.10 The determination results show that the Erjingfang polysaccharide prepared by the present invention has a strong ability to scavenge superoxide anion free radicals, and the scavenging abilities of Polygonatum sibiricum polysaccharide and Lycium barbarum polysaccharide are similar, both of which are significantly lower than the Erjingfang polysaccharide prepared by the present invention.

[0307] In summary, it can be seen that the radical scavenging ability of the Erjingfang polysaccharide prepared in the present invention is higher than that of single-flavor Polygonatum and Lycium barbarum polysaccharides, indicating that there is an obvious synergistic effect between the Polygonatum polysaccharide and Lycium barbarum polysaccharide in the Erjingfang polysaccharide prepared in the present invention.

[0308] Application Example 2: In vivo anti-fatigue experiment of Erjingfang polysaccharide

[0309] In this application example, an in vivo anti-fatigue experiment was conducted on the Erjingfang polysaccharide prepared in Example 38 of the present invention, and the anti-fatigue ability was compared between Polygonatum sibiricum polysaccharide (Comparative Example 4) and Lycium barbarum polysaccharide (Comparative Example 5).

[0310] 1. Experimental methods

[0311] (1) Animal grouping: 60 SPF grade 5-week-old Kunming (KM) male mice were taken and fed in an environment with a temperature of 23-25°C, a light-dark interval of 12h, and a humidity of about 50%, with 6 mice in each cage. Under this condition, they were allowed to eat freely for 3 days. They were randomly divided into a blank group (0.9% saline, marked as group K), a polygonatum polysaccharide intervention group (marked as group H), a wolfberry polysaccharide intervention group (marked as group G), an Erjingfang polysaccharide intervention group (marked as group E) and a positive control (Vc) group. The daily dose of each group was 65 mg / kg. The mice in each group were gavaged for two consecutive weeks, weighed every two days, and the bedding was changed every 3 days.

[0312] (2) Mouse weighted swimming test: 30 minutes after the end of the intragastric administration of polysaccharides on the 13th day, 10 mice were randomly selected from each group, and lead blocks accounting for 5% of their body weight were tied to their tails. The mice were placed in a prepared water tank with a water surface height greater than 35 cm to prevent the tails of the mice from touching the bottom of the water tank, which would affect the accurate calculation of the swimming time. The water temperature was controlled at 25±2℃, and the mice were forced to swim under this environmental condition until they were exhausted and sank to the bottom of the water. They could not return to the surface of the water after 5 seconds, and the weighted swimming time of the mice was recorded.

[0313] (3) Extraction of mouse tissues and analysis of biochemical indicators: 30 minutes after the mice were gavaged with polysaccharides on the 14th day, 10 mice were randomly selected from each group and placed in a prepared water tank with a water surface height greater than 35 cm to prevent the tail of the mouse from touching the bottom of the water tank. The water temperature was controlled at 25±2°C. The mice were allowed to swim without weight for 1 hour under this environmental condition. After swimming, they rested for 30 minutes and were killed by CO2. Blood was taken from the heart and placed in a 1.5 mL EP tube, which was placed flat in a 4°C refrigerator and left to stand overnight. The serum and plasma were then separated and stored at -80°C for later use. The liver, hind leg muscles and bones of the mice were dissected and taken, and stored at -80°C for later use. The blood lactate (BLA), superoxide dismutase (SOD), malondialdehyde (MDA), blood urea nitrogen (BUN), muscle glycogen (MG) and liver glycogen (HG) detection kit instructions were used to determine the serum, liver glycogen and muscle glycogen (in tissues). The BUN concentration, BLA content, MDA content, MG content, HG content, and SOD inhibition rate of the experimental mice were measured, and the data were recorded and analyzed.

[0314] 2. Experimental results

[0315] (1) Comparison of mice’s weighted swimming time Fig.11 As shown, from Fig.11 It can be seen that in the weighted swimming experiment, mice were subjected to Vc as the positive control. The swimming time of the polysaccharide group was significantly longer than that of the blank group, indicating that the polysaccharide intervention groups all had obvious anti-fatigue effects, among which the Erjingfang polysaccharide group had better anti-fatigue effects on mice than Polygonatum sibiricum polysaccharide and Lycium barbarum polysaccharide, and the anti-fatigue effects of Polygonatum sibiricum polysaccharide and Lycium barbarum polysaccharide were similar.

[0316] (2) The measurement results of biochemical indicators are as follows Fig.12 As shown, Fig.12 a in the figure is the result of measuring blood lactic acid content in different groups. The results show that the lactic acid content of the Erjingfang polysaccharide intervention group of the present invention is significantly lower than that of the blank group, indicating that it has a significant anti-fatigue effect and can effectively relieve muscle soreness after exercise. The content of the polygonatum polysaccharide intervention group and the wolfberry polysaccharide intervention group is close, but also significantly lower than the blank group. It proves that the Erjingfang polysaccharide intervention group has the ability to reduce blood lactic acid and can effectively resist fatigue, and the Erjingfang polysaccharide intervention group has a more obvious effect than the polygonatum polysaccharide intervention group and the wolfberry polysaccharide intervention group.

[0317] Fig.12b in the figure is the determination result of urea nitrogen content in different groups. The results show that the urea nitrogen in the VC positive control group is significantly lower than that in the blank group, and the urea nitrogen concentration in the Erjingfang polysaccharide intervention group is also lower than that in the blank group. There is little difference in the reduction of urea nitrogen concentration between the Lycium barbarum polysaccharide intervention group and the Polygonatum sibiricum polysaccharide intervention group, indicating that the Erjingfang polysaccharide intervention group can reduce the urea nitrogen concentration of mice after exercise, and the effect is better than that of the Lycium barbarum polysaccharide intervention group and the Polygonatum sibiricum polysaccharide intervention group, and has obvious anti-fatigue effect.

[0318] Fig.12 c in the figure is the results of liver glycogen content determination in different groups. Fig.12 The d in the figure is the measurement result of muscle glycogen content in different groups. The results show that the muscle glycogen and liver glycogen contents of the Erjingfang polysaccharide intervention group were higher than those of the blank group and the positive control group. The contents of the Polygonatum sibiricum polysaccharide group and the Lycium barbarum polysaccharide group were similar, and their glycogen replenishment abilities were equivalent, but both were lower than those of the Erjingfang polysaccharide intervention group, indicating that the components of the Erjingfang polysaccharide intervention group played a synergistic role.

[0319] Fig.12 e in the figure is the SDS inhibition rate determination result of different groups. The results show that the Erjingfang polysaccharide intervention group has significant SOD inhibition ability, and the inhibition rate is higher than that of the VC positive control group, up to 48.32%, with the most significant effect, followed by Polygonatum sibiricum polysaccharide and Lycium barbarum polysaccharide, indicating that the Erjingfang polysaccharide prepared by the present invention has excellent SOD inhibition ability.

[0320] Fig.12 f in the figure is the measurement result of malondialdehyde content in different groups. The results show that the malondialdehyde content in the blank group increases due to exercise, which makes the mice fatigue quickly, while the malondialdehyde content in the Erjingfang polysaccharide intervention group and the positive control group is significantly reduced, and the malondialdehyde content in the Erjingfang polysaccharide intervention group is lower than that in the Lycium barbarum polysaccharide intervention group and the Polygonatum sibiricum polysaccharide intervention group, and the effect is more obvious, indicating that the Erjingfang polysaccharide prepared by the present invention can reduce the malondialdehyde content in the body, thereby reducing the content of free radicals, and has antioxidant and anti-fatigue effects.

[0321] In summary, the present invention proposes an extraction method and application of Erjingfang polysaccharide, and the present invention combines single factor experiment and response surface optimization experiment to screen out the best Erjingfang polysaccharide extraction process; in addition, the Erjingfang polysaccharide prepared by the present invention is subjected to antioxidant and anti-fatigue tests, and the results show that the Erjingfang polysaccharide prepared by the present invention has synergistic effects in antioxidant and anti-fatigue aspects compared with separately prepared wolfberry polysaccharide and polygonatum polysaccharide, which provides strong support for the development of Erjingfang polysaccharide products.

[0322] The above is a detailed description of the embodiments of the present invention, but the present invention is not limited to the above embodiments. Various changes can be made within the knowledge of ordinary technicians in the relevant technical field without departing from the purpose of the present invention. In addition, the embodiments of the present invention and the features in the embodiments can be combined with each other without conflict.

Claims

1. An application of Erjingfang polysaccharide in the preparation of antioxidant active products or anti-fatigue products, characterized in that: The preparation method of the Erjingfang polysaccharide comprises the following steps: Step S1, mixing wolfberry powder and polygonatum sibiricum powder, adding a first ethanol solution for reflux treatment, and obtaining defatted medicinal material powder; Step S2, adding water to the defatted medicinal material powder at a solid-liquid ratio of 1 g: 10-30 mL, after soaking and ultrasonic treatment, filtering and collecting the filtrate, then adding a second ethanol solution for alcohol precipitation after concentration, collecting the precipitate, and drying to obtain Erjingfang polysaccharide.

2. The use according to claim 1, characterized in that: The mass ratio of the wolfberry powder to the polygonatum powder is 1:0.8-1.

2.

3. The use according to claim 1, characterized in that: The concentration of ethanol in the first ethanol solution is greater than 98%; The mass volume ratio of the mixture of the wolfberry powder and the polygonatum powder to the first ethanol solution is 1g:8-12mL.

4. The use according to claim 1, characterized in that: The reflux treatment time is 0.8 h to 1.2 h.

5. The use according to claim 1, characterized in that: In step S2, the material-liquid ratio is 1 g: 10-20 mL.

6. The use according to claim 1, characterized in that: In step S2, the soaking temperature is 55°C to 65°C, and the soaking time is 20min to 150min.

7. The use according to claim 1, characterized in that: In step S2, the ultrasonic treatment time is 2 min to 65 min.

8. The use according to claim 7, characterized in that: After step S2, the method further includes: mixing the dimethicone polysaccharide with water and activated carbon and performing a decolorization treatment.

9. The use according to claim 8, characterized in that: The final concentration of the activated carbon is 0.0075~0.035g / mL.

10. The use according to claim 8, characterized in that: The temperature of the decolorization treatment is 30°C to 80°C.

11. The use according to claim 8, characterized in that: The decolorization time is 10 min to 60 min.

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