Pseudomonas and its products and applications

Abstract

The present invention relates to a pseudomonad and its products and applications, belonging to the field of bioremediation technology. The present invention obtains a pseudomonad with the function of removing hexavalent uranium through a specific screening method in a natural environment, wherein the strain has a strain collection number of CGMCC No.40409 and is deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration. The present invention also discloses a method for water body remediation using the pseudomonad, which can achieve the maximum hexavalent uranium removal efficiency under optimal conditions, and the uranium removal rate reaches a maximum value of 92.3%.

Description

Technical Field

[0001] The present invention relates to the technical field of bioremediation, and in particular to Pseudomonas and products and applications thereof. Background Art

[0002] Uranium is the most radioactive actinide element in the periodic table, and it is also a substance with high chemical toxicity. It is extremely harmful to the ecological environment and human health, so effective measures need to be taken to control its use and emissions. With the development of industry and nuclear energy, the demand for uranium has continued to increase, which has led to increasingly serious groundwater pollution problems during uranium mining and smelting. Under natural conditions, hexavalent uranium has high solubility and fluidity, so it can diffuse and migrate rapidly, causing serious pollution to groundwater and soil. Hexavalent uranium is highly radioactive, and long-term exposure may cause health risks such as cancer, reproductive problems, and immune system problems. Secondly, hexavalent uranium has high solubility and fluidity under natural conditions, and is easy to diffuse and migrate, causing serious pollution to groundwater and soil. In addition, hexavalent uranium mainly reacts with HCO3 in the blood - Combined with uranium, it is easy to diffuse and mainly deposited in the kidneys, while hexavalent uranium compounds are selectively toxic to the kidneys and may cause damage to renal tubular epithelial cells. Therefore, we need to take effective measures to reduce the pollution of groundwater during uranium mining and smelting to protect the ecological environment and human health.

[0003] At present, there are various remediation technologies for uranium-contaminated wastewater, covering multiple fields such as physical, chemical and biological methods. In addition to biological methods, remediation technologies for uranium-contaminated wastewater also include physical and chemical methods. Physical remediation technologies mainly include adsorption, membrane separation and evaporation. Adsorption is the use of various materials with adsorption effects, such as activated carbon, resin, minerals, etc., to adsorb and remove uranium ions in wastewater. Membrane separation is the use of semipermeable membranes to allow uranium ions to pass through the membrane under pressure to achieve purification. Evaporation is the use of heating to evaporate wastewater for concentration and recovery, but the adsorbents and membranes in physical remediation technology are easily saturated and need to be replaced or regenerated regularly, increasing the treatment cost. Chemical remediation technologies mainly include precipitation and redox methods. Precipitation is the addition of precipitants to wastewater to convert uranium ions into insoluble precipitates, which are then removed by solid-liquid separation. Redox is the use of oxidants or reductants to convert uranium ions into harmless or low-toxic forms, thereby reducing their impact on the environment, but chemical remediation technology may introduce new chemicals and cause secondary pollution. At the same time, these remediation technologies may not be economical and effective for treating uranium pollution in low concentrations and large water bodies. Therefore, a bioremediation technology material or method that solves the above problems is needed. Summary of the invention

[0004] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a Pseudomonas and its products and applications.

[0005] To achieve the above object, the technical solution adopted by the present invention is:

[0006] In the first aspect, the present invention provides a Pseudomonas strain, which was deposited in the General Microbiology Center of the China Culture Collection Administration on November 7, 2022, and the deposit address is the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, with the deposit number CGMCC No. 40409, and is named Pseudomonas sp.DX2.

[0007] As a preferred embodiment of the strain of the present invention, the 16S rDNA of the Pseudomonas is shown in SEQ ID: NO. 1. The determined DNA sequence was compared with homology in the NCBI database, and the result showed that the strain belonged to the genus Pseudomonas.

[0008] The Pseudomonas of the present invention is separated and purified from soil in the natural environment by a screening method, and has the ability to remove hexavalent uranium. The strain was identified as Pseudomonas sp.DX2, and was deposited in the General Microbiology Center of the China Microbiological Culture Collection Administration Committee on November 7, 2022, which is located at the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The Pseudomonas can quickly remove hexavalent uranium, thereby effectively reducing the uranium content in the wastewater. This can not only improve the recovery efficiency of uranium, but also shorten the processing time and improve the processing efficiency. The efficiency of uranium removal by Pseudomonas sp.DX2 provided by the present invention can reach up to 92.3%.

[0009] In a second aspect, the present invention provides a use of Pseudomonas, comprising any one of the following a1)-a2):

[0010] a1) Preparation of products for repairing heavy metal polluted water bodies;

[0011] a2) Remediation of uranium-contaminated water bodies.

[0012] As a preferred embodiment of the present invention, the heavy metal-contaminated water body in a1) is a uranium-contaminated water body, and further, is a hexavalent uranium-contaminated water body.

[0013] The Pseudomonas of the present invention can fix and remove hexavalent uranium through its biological fixation. Hexavalent uranium usually exists in water bodies as soluble uranyl ions, which increases its chance of entering the food chain and may cause greater harm to organisms; hexavalent uranium is not only highly toxic, but also difficult to be removed by conventional treatment processes in nature, so its accumulation may pose a long-term threat to the environment. The present invention uses the mineralization of uranyl ions by Pseudomonas to precipitate uranium ions from water bodies, thereby achieving purification of uranium-contaminated wastewater and reducing the impact of heavy metal uranium on the water ecosystem.

[0014] In a third aspect, the present invention provides a water body restoration product, comprising the Pseudomonas; the concentration of the Pseudomonas in the water body restoration product is 1.25×10 6 CFU / mL-1.25×10 8 CFU / mL.

[0015] As a preferred embodiment of the product of the present invention, preferably, the concentration of Pseudomonas in the water body remediation product is 1.25×10 7 CFU / mL. The total number of Pseudomonas colonies per unit mass or per unit volume in the water body remediation product is 1.25×10 7 CFU. The strain was prepared to contain 1.25×10 7 CFU / mL bacterial suspension can be used to remove hexavalent uranium in sewage. At this concentration of Pseudomonas, the uranium-contaminated water remediation product has the best treatment effect on uranium, with a maximum removal rate of 92.3%, and the final uranium removal is in a dynamic equilibrium process.

[0016] In a fourth aspect, the present invention provides a method for repairing uranium-contaminated water, comprising the following steps: adjusting the pH value of the uranium-contaminated water, and adding the water repair product to the uranium-contaminated water for treatment.

[0017] As a preferred embodiment of the repair method of the present invention, the concentration of Pseudomonas in the water body repair product is 1.25×10 7 CFU / mL; when the uranium content in the uranium contaminated water is 20 mg / L, the addition volume ratio of the water body remediation product is: uranium wastewater volume: water body remediation product volume = 50 mL: (3-10) mL.

[0018] As a preferred embodiment of the remediation method of the present invention, the addition ratio is calculated based on the number of Pseudomonas colonies: uranium content in sewage: number of Pseudomonas colonies = 1 mg / L: (0.1-0.7)×10 7 CFU. More preferably, the ratio is 1 mg / L: (0.125-0.625)×10 7CFU; further, the ratio is 1 mg / L: (0.25-0.375)×10 7 CFU.

[0019] As a preferred embodiment of the remediation method of the present invention, the pH value of the uranium-contaminated water body is adjusted to 7-9 before adding the water remediation product. Under this pH value range, the removal rate of the remediation product for hexavalent uranium wastewater is above 80%. More preferably, the pH value of the uranium-contaminated water body is adjusted to 7. When the pH of the uranium-contaminated water body is adjusted to 7 before adding the water remediation product, the removal rate of hexavalent uranium by Pseudomonas can reach up to 92.3%.

[0020] As a preferred embodiment of the remediation method of the present invention, the treatment time of the water remediation product in the uranium contaminated water body is 3-7 days.

[0021] In a fifth aspect, the present invention provides a method for screening uranium-removing microorganisms, comprising the following steps:

[0022] (1) taking soil from a natural environment, enriching and culturing the microorganisms in the soil, and obtaining a sludge suspension;

[0023] (2) repeatedly screening and culturing the sludge suspension described in step (1) under a N2 environment using a liquid culture medium to obtain a mixed liquid;

[0024] (3) repeatedly applying a solid culture medium to the mixed solution obtained in step (2) to obtain a uranium-removing microorganism;

[0025] The uranium removal microorganisms include Pseudomonas sp.DX2;

[0026] The components of the liquid culture medium and the solid culture medium both include a basic culture medium, a uranyl nitrate solution, a trace amount of metal liquid, an ascorbic acid solution and a sodium thioglycolate solution.

[0027] As a preferred embodiment of the screening method of the present invention, the concentration of the uranyl nitrate solution is 1000 mg / L. Uranyl nitrate, also known as "uranyl nitrate", has a chemical formula of UO2(NO3)2·6H2O, wherein the valence of the uranium element in uranyl nitrate is +6. The present invention uses uranyl nitrate as the main component of the conditioned medium, which can simulate the conditions of sewage containing hexavalent uranium; through the high concentration of uranium element, those microorganisms that can use uranyl nitrate as an energy source or electron acceptor or those microorganisms that have tolerance or degradation ability in a uranium-contaminated environment are screened out.

[0028] As a preferred embodiment of the screening method of the present invention, the composition of the basic culture medium is: KH2PO4: 0.5g / L, NH4Cl: 1.0g / L, CaCl2·6H2O: 0.06g / L, Na2SO4: 4.5g / L, yeast extract: 1.0g / L, MgSO4·7H2O: 0.06g / L, Na3C6H5O7·2H2O: 0.3g / L, C3H5O3Na: 6g / L, FeSO4·7H2O: 0.01g / L, and resazurin: 0.001g / L.

[0029] As a preferred embodiment of the screening method of the present invention, the concentration of the ascorbic acid solution is 100g / L. Ascorbic acid itself is a substance that can remove free radicals, reduce oxidative stress, and thus protect cells from oxidative damage. By adding a certain amount of ascorbic acid to the conditioned medium, it can act as an electron donor, participate in the respiratory process of microorganisms, and provide energy for them. This helps microorganisms grow better in an environment with limited nutrients. It promotes growth and reproduction and tolerance to heavy metals, helps protect the environment, control pollution, and improves the application value of the microorganisms obtained by screening.

[0030] As a preferred embodiment of the screening method of the present invention, the concentration of the sodium thioglycolate solution is 100 g / L. In the screening of microorganisms, sodium thioglycolate can well buffer the culture medium so that the pH value of the culture medium will not change significantly, thereby ensuring the stability of the microbial growth environment; at the same time, it alleviates the toxicity of high heavy metal content to microorganisms.

[0031] As a preferred embodiment of the screening method of the present invention, the components in the trace metal liquid include, by mass concentration, 35.0 mg / L CoCl2·6H2O, 0.20 mg / L CuCl2, 6.0 mg / L H3BO3, 25.0 mg / LMnCl2·4H2O, 3.0 mg / L Na2MoO4·2H2O, 2.0 mg / L NiCl2·2H2O, and 2.5 mg / L ZnCl2.

[0032] As a preferred embodiment of the screening method of the present invention, in step (2), the screening culture comprises the following steps:

[0033] (2a) adding 10 mL of the sludge suspension into the liquid culture medium, blowing N2 for 20 min and then sealing; culturing in an oscillator at a temperature of 30° C. and a rotation speed of 150 r / min under a N2 environment for 7 days to obtain a culture mixture;

[0034] (2b) Take 1 mL of the culture mixture and place it in a newly prepared liquid culture medium for a new round of culture. The culture conditions for the new round are the same as those in step (2a). Repeat until the seventh round to obtain the seventh round of culture mixture.

[0035] As a preferred embodiment of the screening method of the present invention, in step (3), the coating culture comprises the following steps:

[0036] (3a) taking the seventh round of culture mixture obtained in step (2b), adding it to a solid culture medium for spreading culture at 30° C. for 20 days to obtain a single colony;

[0037] (3b) picking a single colony obtained in step (3a) and inoculating it into a serum bottle containing a liquid culture medium, and then culturing it in an oscillator at a temperature of 30° C. and a rotation speed of 150 r / min for 7 days to obtain a culture mixture to be coated;

[0038] (3c) adding the culture mixture to be coated obtained in step (3b) to a newly prepared solid culture medium to carry out a new round of coating culture, and repeating to the seventh round to obtain a bacterial solution of uranium-removing microorganisms.

[0039] As a preferred embodiment of the screening method of the present invention, in steps (3a)-(3c), the coating culture and inoculation operations are all carried out in an anaerobic glove box. In the screening method of the present invention, the whole process maintains anaerobic conditions, wherein the anaerobic liquid screening simulates the heavy metals in the uranium contaminated wastewater and the conditions of low oxygen content, and can further screen out microorganisms that can remove uranyl ions. Solid culture medium can provide better space and restrictions for microbial growth, so that microorganisms form visible colonies, which are convenient for counting and separation. Through multiple rounds of continuous screening, the target strain can be avoided from experiencing a process of reduced adaptability and decline. Continuous screening can maintain the vitality and adaptability of microorganisms, avoiding the problem of decline in the ability of uranium removal strains.

[0040] Compared with the prior art, the present invention has the following beneficial effects:

[0041] 1. The present invention provides a pseudomonad, which is deposited in the China General Microbiological Culture Collection Center with a deposit number of CGMCC No. 40409. The pseudomonad is screened in a high sulfate environment and has the function of removing highly toxic hexavalent uranium. The pseudomonad has a high efficiency in removing uranium, and under optimal conditions, the maximum removal rate of hexavalent uranium is 92.3%.

[0042] 2. The present invention provides a product for the restoration of uranium-contaminated water bodies, wherein the total number of Pseudomonas colonies per unit mass or per unit volume is 1.25×10 7CFU. This remediation product can be used to remove hexavalent uranium in sewage. At the same time, the preparation and application methods of this product are simple, and there is no need to add other additives to cause secondary pollution of the water body.

[0043] 3. The present invention provides a method for repairing uranium-contaminated water bodies. The method can utilize Pseudomonas sp.DX2 to quickly remove uranyl ions in water bodies, effectively reduce the concentration of uranium, and achieve the purpose of repairing uranium-contaminated water bodies. Compared with traditional physical and chemical methods, microbial remediation methods produce fewer harmful substances during the treatment process and have less impact on the environment and human health. The method is simple to operate, has low technical requirements for operators, and is easy to promote and apply. Finally, the removal rate of hexavalent uranium in water bodies with a uranium concentration of 20 mg / L is as high as 92.3%. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 This is the colony morphology of Pseudomonas sp.DX2;

[0045] Figure 2 is the uranium removal rate of the strain under different reaction conditions, where (a) is the effect of initial uranium concentration on the uranium removal performance of Pseudomonas; (b) is the effect of pH value on the uranium removal performance of Pseudomonas; (c) is the effect of bacterial solution dosage on the uranium removal performance of Pseudomonas; (d) is the effect of treatment time on the uranium removal performance of Pseudomonas. DETAILED DESCRIPTION

[0046] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0047] Example 1 Screening method for Pseudomonas sp.DX2

[0048] (1) Configuration of screening medium The specific components of the screening medium are shown in Table 1 below:

[0049] Table 1 Composition of screening medium

[0050] Element content Basic culture medium 90.0mL 1000mg / L uranyl nitrate 2.0mL Trace metal liquid 0.1mL 100g / L Ascorbic acid solution 0.1mL 100g / L sodium thioglycolate solution 0.1mL

[0051] The specific components of the basic culture medium are shown in Table 2 below:

[0052] Table 2 Composition of basal medium

[0053]

[0054]

[0055] The specific composition of the trace metal liquid is shown in Table 3 below:

[0056] Table 3 Composition of trace metal liquid

[0057] Element content <![CDATA[CoCl2·6H2O]]> 35.0mg / L <![CDATA[CuCl2]]> 0.20mg / L <![CDATA[H3BO3]]> 6.0mg / L <![CDATA[MnCl2·4H2O]]> 25.0mg / L <![CDATA[Na2MoO4·2H2O]]> 3.0mg / L <![CDATA[NiCl2·2H2O]]> 2.0mg / L <![CDATA[ZnCl2]]> 2.5mg / L

[0058] The steps of separating and purifying the hexavalent uranium removal bacteria from the natural environment are as follows:

[0059] (1) Take 100 g of soil from the natural environment, bring it back to the laboratory and immediately store it in a 4°C refrigerator for later use;

[0060] (2) Add 900 mL of basal culture medium to an open glass bottle with a volume of 1000 mL, add 50 g of soil to the open glass bottle, and seal it with a rubber stopper; then place it in an environment with a temperature of 30°C and stir it at a speed of 100 r / min for enrichment and acclimatization culture until the suspension turns ink color and there is a rotten egg smell at the bottle mouth. During the culture period, the basal culture medium is replaced regularly to maintain an adequate supply of nutrients in the glass bottle;

[0061] (3) Prepare 100 ml of screening culture medium in a 250 ml conical flask, blow N2 for 20 min, and then seal with a rubber stopper. Under N2 environment, add 10 ml of the sludge suspension obtained by acclimatization in step (2) to the conical flask, and then place it in an oscillator at a temperature of 30 ° C and a speed of 150 r / min for 7 days. Take 1 ml of the mixed solution in the conical flask and transfer it to a new conical flask containing 100 ml of screening culture medium, and culture it under the same conditions, and repeat it for the seventh time.

[0062] (4) adding 450 mL of basal culture medium, 0.5 mL of trace metal liquid, 0.5 mL of ascorbic acid solution with a mass concentration of 100 g / L, 0.5 mL of sodium thioglycolate solution with a mass concentration of 100 g / L, and 7.5 g of agar into a conical flask with a volume of 1000 mL, sterilizing it in an autoclave at a temperature of 121° C. for 20 minutes, cooling it to 60° C., and then pouring it into 7 sterile culture dishes with a diameter of 9 cm in an ultra-clean workbench, adding 0.5 mL of uranyl nitrate solution with a mass concentration of 1000 mg / L to culture dishes 1-7 in the ultra-clean workbench, respectively, tilting and rotating the culture dishes back and forth to evenly distribute the uranyl nitrate solution, and transferring the 7 culture dishes to an anaerobic glove box after cooling them in the ultra-clean workbench to obtain 7 screening culture dishes containing uranyl nitrate solution;

[0063] (5) adding 98 mL of basal culture medium to 7 serum bottles with a volume of 250 mL respectively, sterilizing at 121° C., and placing in an anaerobic glove box, and adding the remaining screening culture medium components to the 7 serum bottles in the anaerobic glove box, including 2 mL of uranyl nitrate solution with a mass concentration of 1000 mg / L, 0.1 mL of trace metal liquid, 0.1 mL of ascorbic acid solution with a mass concentration of 100 g / L, and 0.1 mL of sodium thioglycolate solution with a mass concentration of 100 g / L, to obtain 7 serum bottles containing screening culture medium;

[0064] (6) Take 1 mL of the mixed solution in the conical flask cultured to the seventh round in step (3) and add it to the screening culture dish obtained in step (4), tilt and rotate the culture dish back and forth to evenly distribute the inoculated bacterial solution, seal it, and place it in an incubator at a temperature of 30° C. and culture it for 20 days; after 20 days, pick the colonies in the culture dish in an anaerobic glove box and inoculate them into the serum bottle containing the screening culture medium prepared in step (5), and then place it in an oscillator at a temperature of 30° C. and a rotation speed of 150 r / min and culture it for 7 days.

[0065] (7) After 7 days, take the mixed solution in the serum bottle and add it to a new screening culture dish. After coating, repeat the operation of step (6) for screening culture. After 20 days, pick the colonies in the new screening culture dish in the anaerobic glove box and inoculate them into a serum bottle with a new screening culture medium for culture. A total of 7 rounds of screening culture are performed. Finally, the bacterial solution in the serum bottle contains pure strains that can efficiently remove hexavalent uranium.

[0066] The pure strain of hexavalent uranium was identified, and the 16S rDNA sequencing of the strain was completed by Beijing Bomed Gene Technology Co., Ltd. The strain 16s rDNA was measured as shown in the sequence ID: NO.1 sequence in the sequence list. The measured DNA sequence was compared with the NCBI database for homology, and the results showed that the strain belonged to the genus Pseudomonas. After multiple coatings and cultivations on the culture medium, pure bacteria were obtained, and the colony morphology was as follows Figure 1 As shown. The colonies are round, milky white, opaque, and have neat edges.

[0067] Examples 2-9 and Comparative Examples 1-9

[0068] Examples 2-9 and Comparative Examples 1-9 were tested for the removal of hexavalent uranium using different conditions. The specific reaction conditions are shown in Table 1. The isolated and purified hexavalent uranium removal strain Pseudomonas sp.DX2 was prepared into a bacterial suspension containing 1.25×10 7 CFU / mL, and the uranium-containing wastewater system for the removal test was 50mL.

[0069] Table 1 Hexavalent uranium removal test conditions for Examples 2-9 and Comparative Examples 1-9

[0070]

[0071] The uranium content in the water after the reaction under the conditions of Examples 2-9 and Comparative Examples 1-9 was detected. The results are as follows Figure 2 shown.

[0072] according to Figure 2 (a), the results of Example 2 and Comparative Examples 1-4 show that when the initial uranium concentration in the uranium contaminated water is 10 mg / L-50 mg / L, the removal rate of hexavalent uranium is above 50%. When the initial uranium concentration in the uranium contaminated water is 20 mg / L, the removal rate of uranium by Pseudomonas reaches the maximum value, indicating that at this initial uranium concentration, Pseudomonas can maximize the removal of hexavalent uranium through its biological action, and the uranium removal rate reaches a maximum value of 85.3%.

[0073] according to Figure 2 (b) The results of Example 2, Examples 4-5 and Comparative Examples 5-6 show that when the pH of the uranium wastewater is less than 7 and is in an acidic environment, the removal rate of uranium by Pseudomonas is low; when the pH of the uranium wastewater is greater than 7, the removal rate of hexavalent uranium by Pseudomonas reaches more than 75%-80%, among which the best treatment effect is achieved when the pH of the uranium wastewater is 7.

[0074] according to Figure 2 (c) The results of Example 3, Examples 6-7 and Comparative Examples 7-8 show that the concentration of Pseudomonas in the water body remediation product is 1.25×10 7 CFU / mL, and when the initial uranium concentration in the uranium-contaminated water is 20mg / L, the dosage of the remediation product is 3-10mL, which has a good effect. When the dosage is less than 3mL, the concentration of Pseudomonas is not enough to efficiently treat hexavalent uranium; and as the dosage increases to more than 5mL, the treatment efficiency of Pseudomonas for hexavalent uranium reaches more than 80%, and finally reaches a dynamic balance. When the bacterial solution dosage is 5mL, the uranium removal rate reaches a maximum of 92.3%.

[0075] according to Figure 2 (d) The results of Example 3, Examples 8-9 and Comparative Example 9 show that, under the preferred concentration and conditions, when the treatment time is 1-3 days, as the treatment time of the repair product increases, the treatment efficiency of Pseudomonas for hexavalent uranium increases; when the treatment time is on the 3rd day, the treatment efficiency of Pseudomonas for hexavalent uranium reaches a maximum value of 92.3%; thereafter, the removal of uranium has been in a dynamic equilibrium process, and the treatment efficiency of hexavalent uranium is maintained at 85%-90%.

[0076] In summary, in 50 mL of uranium-contaminated water, when the concentration of Pseudomonas in the water remediation product was 1.25×10 7CFU / mL, and when the initial uranium concentration in the uranium-contaminated water was 20 mg / L, the pH of the uranium-contaminated water was adjusted to 7, and 5 mL of 1.25×10 7 CFU / mL of the repair product can achieve the best hexavalent uranium removal effect within 3 days, with the removal rate reaching a maximum of 92.3%.

[0077] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A Pseudomonas, characterized in that The strain was deposited in the General Microbiology Center of China Culture Collection Administration on November 7, 2022. The deposit address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, Building 1, Beichen West Road, Chaoyang District, Beijing. The deposit number is CGMCC No. 40409, and it is named Pseudomonas sp.DX2.

2. The Pseudomonas according to claim 1, characterized in that The 16S rDNA of the Pseudomonas is shown in SEQ ID: NO.

1.

3. Use of the Pseudomonas described in any one of claims 1-2 in remediation of uranium-contaminated water.

4. A water body restoration product, characterized in that: The method comprises the Pseudomonas according to any one of claims 1 to 2; wherein the concentration of the Pseudomonas in the product for removing uranium pollution from water is 1.25×10 6 -1.25×10 8 CFU / mL.

5. The product according to claim 4, characterized in that The concentration of Pseudomonas in the product for removing uranium pollution from water is 1.25×10 7 CFU / mL.

6. A method for repairing uranium-contaminated water, characterized in that: The following steps are involved: The pH value of the uranium-contaminated water body is adjusted, and the water body remediation product according to claim 4 or 5 is added to the uranium-contaminated water body for treatment.

7. The repair method according to claim 6, characterized in that: The concentration of Pseudomonas in the water remediation product is 1.25×10 7 CFU / mL; when the uranium content in the uranium-contaminated water is 20 mg / L, the addition volume ratio of the water body remediation product is: uranium-contaminated water volume: water body remediation product volume = 50 mL: (3-10) mL.

8. The repair method according to claim 6, characterized in that: Before adding the water body remediation product, the pH value of the uranium contaminated water body is adjusted to 7-9; the treatment time of the water body remediation product is 3-7 days.

Images