A composition of hypoglycemic plant extract and preparation method thereof

Abstract

The present invention provides a composition of hypoglycemic plant extracts, which comprises the following components in weight: 10 to 20 parts by weight of mulberry leaf extract, 2 to 5 parts by weight of cinnamon extract, 0.5 to 2 parts by weight of loquat leaf extract, 1 to 3 parts by weight of astragalus total saponins, 1 to 3 parts by weight of kudzu root polysaccharide extract, 0.05 to 0.1 parts by weight of chlorogenic acid, and 0.2 to 0.5 parts by weight of salvianolic acid B. After the composition of the hypoglycemic plant extracts of the present invention is matched, loquat leaf extract, astragalus total saponins chlorogenic acid, and salvianolic acid can promote the improvement of the efficacy of each component. Mulberry leaf extract mainly prevents the absorption of sugar from the source and directly reduces postprandial blood sugar. Corosolic acid contained in loquat leaf extract can promote the absorption and utilization of glucose by cells, thereby reducing blood sugar.

Description

Technical Field

[0001] The invention relates to the application field of plant extracts, and in particular to a blood sugar lowering plant extract composition and a preparation method thereof. Background Art

[0002] Mulberry leaf extract has multiple biological activities such as antioxidant, anti-tumor, anti-viral, and immune regulation. Mulberry leaves are often used to extract alkaloids and flavonoid compounds. Mulberry leaf extract is mostly used in the preparation of health foods. However, the intake of sugars needs to be controlled when mulberry leaf extract is consumed. Therefore, the efficacy of health foods prepared mainly with mulberry leaf extract needs to be further improved. Summary of the invention

[0003] The purpose of the present invention is to overcome the shortcomings of the prior art and provide a composition of a blood sugar-lowering plant extract and a preparation method thereof.

[0004] To achieve the above-mentioned purpose, the technical solution adopted by the present invention is: a composition of a hypoglycemic plant extract, which comprises the following components in weight parts: 10 to 20 weight parts of mulberry leaf extract, 2 to 5 weight parts of cinnamon extract, 0.5 to 2 weight parts of loquat leaf extract, 1 to 3 weight parts of astragalus total saponins, 1 to 3 weight parts of kudzu polysaccharide extract, 0.05 to 0.1 weight parts of chlorogenic acid and 0.2 to 0.5 weight parts of salvianolic acid B.

[0005] The above-mentioned combination of blood sugar lowering plant extracts, loquat leaf extract, astragalus total saponin chlorogenic acid, and salvianolic acid can promote the improvement of the efficacy of each component. Mulberry leaf extract mainly prevents the absorption of sugar from the source and directly reduces postprandial blood sugar. The corosolic acid contained in the loquat leaf extract can promote the absorption and utilization of glucose by cells, thereby lowering blood sugar. Loquat leaf extract and chlorogenic acid have antibacterial effects. Salvianolic acid B not only has an antioxidant effect but also can promote the inhibition of the composition on α-amylase and α-glucosidase, which is more conducive to preventing the absorption of sugar from the source and directly reducing postprandial blood sugar.

[0006] Preferably, the preparation method of loquat leaf extract comprises the following steps:

[0007] (1) drying loquat leaves and grinding them into powder, adding 0.08-0.12 mol / L sodium hydroxide solution in a ratio of 1:(30-50) between solid and liquid, and ultrasonically extracting at 450-500 W power at 35-42° C. for 20-40 minutes;

[0008] (2) Solid-liquid separation: collecting the supernatant, adjusting the pH to the isoelectric point of 3.5 with hydrochloric acid, standing for 25 to 35 minutes, collecting the precipitate by centrifugation, washing with distilled water, and freeze-drying to obtain the loquat leaf extract.

[0009] When loquat leaf extract is combined with other components such as tannic acid B and mulberry leaf extract, it can promote the inhibition of the composition on α-amylase and α-glucosidase, which is more conducive to preventing the absorption of sugar from the source and directly reducing postprandial blood sugar.

[0010] Preferably, the method for preparing the pueraria polysaccharide extract comprises the following steps:

[0011] (a) mixing kudzu root powder and water in a solid-liquid ratio of 1:(30-50), and ultrasonically extracting at 70-85° C. and 150-250 W for 20-40 minutes;

[0012] (b) concentrating under reduced pressure, adding 75% to 85% by volume ethanol for alcohol precipitation, standing at 4° C., centrifuging, repeatedly washing the precipitate with anhydrous ethanol, and vacuum freeze-drying to obtain a pueraria polysaccharide extract.

[0013] Preferably, the composition of the blood sugar-lowering plant extract comprises the following components in parts by weight: 10 to 20 parts by weight of mulberry leaf extract, 2 to 5 parts by weight of cinnamon extract, 0.5 to 2 parts by weight of loquat leaf extract, 1 to 3 parts by weight of astragaloside total saponins, 1 to 3 parts by weight of kudzu root polysaccharide extract, 0.05 to 0.1 parts by weight of chlorogenic acid, 0.2 to 0.5 parts by weight of salvianolic acid B, 15 to 30 parts by weight of red bean powder, 15 to 30 parts by weight of coix seed powder, 15 to 30 parts by weight of buckwheat powder, 15 to 30 parts by weight of bean powder and 15 to 30 parts by weight of propolis powder.

[0014] The above-mentioned combination of hypoglycemic plant extracts, loquat leaf extract, astragalus total saponin chlorogenic acid, and salvianolic acid can promote the improvement of the efficacy of each component. Mulberry leaf extract mainly prevents the absorption of sugar from the source and directly reduces postprandial blood sugar. The corosolic acid contained in the loquat leaf extract can promote the absorption and utilization of glucose by cells, thereby lowering blood sugar. Loquat leaf extract and chlorogenic acid have antibacterial effects. Salvianolic acid B can not only play an antioxidant role but also promote the inhibition of α-amylase and α-glucosidase by the composition, which is more conducive to preventing the absorption of sugar from the source and directly reducing postprandial blood sugar. Further combination with red bean powder, coix seed powder, buckwheat powder, bean powder, and propolis powder is beneficial to converting sugar components into glucose, which can ensure the intake of nutrients and lower blood sugar.

[0015] Preferably, the weight ratio of mulberry leaf extract, loquat leaf extract, astragalus total saponins, chlorogenic acid and salvianolic acid in the composition of blood sugar-lowering plant extracts is 15: (0.8-1.5): (1.2-1.5): (0.05-0.3): (0.30-0.35).

[0016] Through research, it was found that when the weight ratio of mulberry leaf extract, loquat leaf extract, astragalus total saponins, chlorogenic acid and salvianolic acid meets the above ratio, they can promote the improvement of the efficacy of each component. Mulberry leaf extract mainly blocks the absorption of sugar from the source and directly lowers postprandial blood sugar. The corosolic acid contained in loquat leaf extract can promote the absorption and utilization of glucose by cells, thereby lowering blood sugar. It has better functions of lowering blood sugar and providing nutritional components.

[0017] Preferably, the weight ratio of mulberry leaf extract, loquat leaf extract, astragalus total saponins, chlorogenic acid and salvianolic acid in the composition of blood sugar-lowering plant extracts is 15:(1-1.2):(1.2-1.5):0.08:0.35.

[0018] The present invention also provides a method for preparing any of the above-mentioned hypoglycemic plant extract compositions, characterized in that the method comprises the following steps:

[0019] (I) drying loquat leaves and grinding them into powder, adding 0.08-0.12 mol / L sodium hydroxide solution in a solid-liquid ratio of 1:(30-50), and ultrasonically extracting at 450-500 W power at 35-42° C. for 20-40 minutes;

[0020] (II) collecting the supernatant by solid-liquid separation, adjusting the pH to the isoelectric point of 3.5 with hydrochloric acid, allowing to stand for 25 to 35 minutes, collecting the precipitate by centrifugation, washing with distilled water, and freeze-drying to obtain a loquat leaf extract;

[0021] (III) mixing kudzu root powder and water in a solid-liquid ratio of 1:(30-50), and ultrasonically extracting at 70-85° C. and 150-250 W for 20-40 minutes;

[0022] (VI) concentrating under reduced pressure, adding 75% to 85% by volume ethanol for alcohol precipitation, standing at 4° C., centrifuging, repeatedly washing the precipitate with anhydrous ethanol, and vacuum freeze-drying to obtain a pueraria polysaccharide extract;

[0023] (V) Mulberry leaf extract, cinnamon extract, loquat leaf extract, astragalus total saponin, kudzu root polysaccharide extract, red bean powder, coix seed powder, buckwheat powder, soybean powder, and propolis powder are mixed according to weight ratio, and then vacuum dried at below 20° C. for 8 to 15 minutes, and chlorogenic acid and salvianolic acid B are added and mixed to obtain a blood sugar-lowering plant extract composition.

[0024] The beneficial effects of the present invention are as follows: the present invention provides a composition of a blood sugar lowering plant extract. After the composition of the blood sugar lowering plant extract of the present invention is matched, loquat leaf extract, astragalus total saponin chlorogenic acid, and salvianolic acid can promote the improvement of the efficacy of each component. Mulberry leaf extract mainly prevents the absorption of sugar from the source and directly reduces postprandial blood sugar. Corosolic acid contained in loquat leaf extract can promote the absorption and utilization of glucose by cells, thereby lowering blood sugar. Loquat leaf extract and chlorogenic acid have antibacterial effects. Salvianolic acid B can not only play an antioxidant role but also promote the inhibition of the composition on α-amylase and α-glucosidase, which is more conducive to preventing the absorption of sugar from the source and directly reducing postprandial blood sugar. DETAILED DESCRIPTION

[0025] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0026] Example 1

[0027] As a composition of a hypoglycemic plant extract in an embodiment of the present invention, the composition of the hypoglycemic plant extract includes the following components in weight parts: 15 weight parts of mulberry leaf extract, 3 weight parts of cinnamon extract, 1 weight part of loquat leaf extract, 1.2 weight parts of astragalus total saponins, 2 weight parts of kudzu polysaccharide extract, 0.08 weight parts of chlorogenic acid and 0.3 weight parts of salvianolic acid B.

[0028] The preparation method of the loquat leaf extract comprises the following steps:

[0029] (1) The loquat leaves were dried and powdered, and 0.10 mol / L sodium hydroxide solution was added at a solid-liquid ratio of 1:40, and ultrasonic extraction was performed at 40° C. and 480 W power for 30 minutes;

[0030] (2) The supernatant was collected by solid-liquid separation, and the pH was adjusted to the isoelectric point of 3.5 with 1 mol / L hydrochloric acid. After standing for 30 min, the precipitate was collected by centrifugation, washed with distilled water, and freeze-dried to obtain loquat leaf extract.

[0031] The preparation method of the pueraria polysaccharide extract comprises the following steps:

[0032] (a) After pueraria root (dried root of Pueraria lobata (Willd.) Ohwi, a plant of the Leguminosae family) was ground into powder, the 60-mesh pueraria root powder was mixed with water at a solid-liquid ratio of 1:40, and ultrasonically extracted at 78°C at a power of 200 W for 30 minutes;

[0033] (b) concentrating under reduced pressure, adding 80% volume ethanol for alcohol precipitation, standing at 4° C., centrifuging, repeatedly washing the precipitate with anhydrous ethanol, and vacuum freeze-drying to obtain a pueraria polysaccharide extract.

[0034] Example 2

[0035] As a composition of a hypoglycemic plant extract in an embodiment of the present invention, the composition of the hypoglycemic plant extract includes the following components in weight parts: 12 weight parts of mulberry leaf extract, 5 weight parts of cinnamon extract, 1 weight part of loquat leaf extract, 1.2 weight parts of astragalus total saponins, 2 weight parts of kudzu polysaccharide extract, 0.08 weight parts of chlorogenic acid and 0.3 weight parts of salvianolic acid B.

[0036] The rest is the same as in Example 1.

[0037] Example 3

[0038] As a hypoglycemic plant extract composition of an embodiment of the present invention, the hypoglycemic plant extract composition includes the following components in weight parts: 18 weight parts of mulberry leaf extract, 2.5 weight parts of cinnamon extract, 1 weight part of loquat leaf extract, 1.2 weight parts of astragalus total saponins, 2 weight parts of kudzu polysaccharide extract, 0.08 weight parts of chlorogenic acid and 0.3 weight parts of salvianolic acid B.

[0039] The rest is the same as in Example 1.

[0040] Example 4

[0041] As a hypoglycemic plant extract composition of an embodiment of the present invention, the hypoglycemic plant extract composition includes the following components in weight parts: 18 weight parts of mulberry leaf extract, 2.5 weight parts of cinnamon extract, 1.8 weight parts of loquat leaf extract, 1.2 weight parts of astragalus total saponins, 2 weight parts of kudzu polysaccharide extract, 0.08 weight parts of chlorogenic acid and 0.3 weight parts of salvianolic acid B.

[0042] The rest is the same as in Example 1.

[0043] Example 5

[0044] As a composition of a hypoglycemic plant extract according to an embodiment of the present invention, the composition of the hypoglycemic plant extract includes the following components in parts by weight: 15 parts by weight of mulberry leaf extract, 3 parts by weight of cinnamon extract, 1 part by weight of loquat leaf extract, 1.2 parts by weight of astragalus total saponins, 2 parts by weight of kudzu polysaccharide extract, 0.08 parts by weight of chlorogenic acid, 0.3 parts by weight of salvianolic acid B, 20 parts by weight of red bean powder, 20 parts by weight of coix seed powder, 20 parts by weight of tartary buckwheat powder, 20 parts by weight of bean powder and 20 parts by weight of propolis powder.

[0045] The rest is the same as in Example 1.

[0046] Example 6

[0047] As a composition of a hypoglycemic plant extract according to an embodiment of the present invention, the composition of the hypoglycemic plant extract includes the following components in parts by weight: 12 parts by weight of mulberry leaf extract, 5 parts by weight of cinnamon extract, 1 part by weight of loquat leaf extract, 1.2 parts by weight of astragalus total saponins, 2 parts by weight of kudzu polysaccharide extract, 0.08 parts by weight of chlorogenic acid, 0.3 parts by weight of salvianolic acid B, 20 parts by weight of red bean powder, 20 parts by weight of coix seed powder, 20 parts by weight of tartary buckwheat powder, 20 parts by weight of bean powder and 20 parts by weight of propolis powder.

[0048] The rest is the same as in Example 1.

[0049] Comparative Example 1

[0050] As a blood sugar lowering plant extract composition of comparative example 1 of the present invention, the only difference between this comparative example and example 1 is that it does not contain loquat leaf extract.

[0051] Comparative Example 2

[0052] As a hypoglycemic plant extract composition of comparative example 1 of the present invention, the only difference between this comparative example and example 1 is that it does not contain astragalus total saponins.

[0053] Comparative Example 3

[0054] As a hypoglycemic plant extract composition of Comparative Example 1 of the present invention, the only difference between this comparative example and Example 1 is that it does not contain chlorogenic acid.

[0055] Comparative Example 4

[0056] As a hypoglycemic plant extract composition of comparative example 1 of the present invention, the only difference between this comparative example and example 1 is that it does not contain salvianolic acid B.

[0057] Experimental methods

[0058] 1. Experimental Materials

[0059] Food-grade mulberry leaf extract was purchased from Jiangsu Caiwei Biotechnology Co., Ltd., food-grade cinnamon extract was purchased from Guangzhou Huayu Biotechnology Co., Ltd., astragalus total saponins were purchased from Shanghai Yuanye Biotechnology Co., Ltd., and loquat leaf extract was purchased from Xi'an Anao Biotechnology Co., Ltd.

[0060] 2. Experimental Methods

[0061] 1. Determination of α-glucosidase inhibitory activity

[0062] Mix 200 μL of the sample solution with an equal volume of α-glucosidase (7000 U mL -1) was mixed thoroughly and reacted at 37°C for 15 min. Then an equal volume of 2.5 mmol·L -1 p-Nitrophenyl-α-D-pyranose solution was reacted at 37°C for 20 min, and 5 mL of 0.10 mol·L -1 The reaction was terminated with Na2CO3 solution and the absorbance was measured at 400nm. The α-glucosidase inhibition rate was calculated as follows:

[0063] α-Glucosidase inhibition rate (%) = (1-(A0-A1) / A2) × 100%

[0064] Wherein: A0 is the OD400 after the reaction of the mixture of sample solution, enzyme and nitrophenyl-α-D-pyranose glucose solution; A1 is the OD400 after the reaction of the buffer solution replacing the α-glucosidase solution; A2 is the OD400 after the reaction of the buffer solution replacing the sample solution.

[0065] 2. Determination of α-amylase inhibitory activity

[0066] 250 μL of the sample solution to be tested and an equal volume of α-amylase solution (1.4 U mL -1 ) was fully mixed, reacted at 37°C for 10 min, then an equal volume of 1% starch solution was added thereto, reacted at 37°C for 10 min, 500 μL of DNS was added to the reaction system to terminate the reaction, and the mixture was quickly cooled to room temperature after boiling in a water bath for 5 min, and 5 mL of distilled water was added to dilute the mixture, and the absorbance was measured at 540 nm. The α-amylase inhibition rate was calculated by the formula shown in the following formula:

[0067] α-Amylase inhibition rate (%) = (1-(A0-A1) / A2) × 100%

[0068] Wherein: A0 is the OD540 after the reaction of the sample solution, enzyme and starch mixture; A1 is the OD540 after the reaction of the buffer solution replacing the α-amylase solution; A2 is the OD540 after the reaction of the buffer solution replacing the sample solution.

[0069] The hypoglycemic plant extract compositions of Examples 1-6 and Comparative Examples 1-4 were prepared into 100 g / L solutions with deionized water, and used as test samples to test the inhibition rates of α-glucosidase and α-amylase.

[0070] The experimental results are shown in Table 1.

[0071] Table 1 α-glucosidase and α-amylase inhibition rates of the combination of blood sugar-lowering plant extracts.

[0072]

[0073]

[0074] As can be seen from Table 1, the combination of loquat leaf extract, astragalus total saponin chlorogenic acid and salvianolic acid in the blood sugar lowering plant extract of the present invention can promote the improvement of the efficacy of each component. Mulberry leaf extract mainly prevents the absorption of sugar from the source and directly reduces postprandial blood sugar. The corosolic acid contained in the loquat leaf extract can promote the absorption and utilization of glucose by cells, thereby lowering blood sugar. The loquat leaf extract and chlorogenic acid have an antibacterial effect. In addition to having an antioxidant effect, salvianolic acid B can also promote the inhibition of the composition on α-amylase and α-glucosidase, which is more conducive to preventing the absorption of sugar from the source and directly reducing postprandial blood sugar.

[0075] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A composition of a hypoglycemic plant extract, characterized in that: The hypoglycemic plant extract composition comprises 10 to 20 parts by weight of mulberry leaf extract, 2 to 5 parts by weight of cinnamon bark extract, 0.5 to 2 parts by weight of loquat leaf extract, 1 to 3 parts by weight of astragalus total saponins, 1 to 3 parts by weight of kudzu root polysaccharide extract, 0.05 to 0.1 parts by weight of chlorogenic acid, and 0.2 to 0.5 parts by weight of salvianolic acid B; The preparation method of the loquat leaf extract comprises the following steps: (1) drying loquat leaves and grinding them into powder, adding 0.08-0.12 mol / L sodium hydroxide solution in a ratio of 1:(30-50) between solid and liquid, and ultrasonically extracting at 450-500 W power at 35-42° C. for 20-40 minutes; (2) collecting the supernatant by solid-liquid separation, adjusting the pH to the isoelectric point of 3.5 with hydrochloric acid, allowing to stand for 25 to 35 minutes, collecting the precipitate by centrifugation, washing with distilled water, and freeze-drying to obtain a loquat leaf extract; The preparation method of the pueraria polysaccharide extract comprises the following steps: (a) mixing kudzu root powder and water in a solid-liquid ratio of 1:(30-50), and ultrasonically extracting at 70-85° C. and 150-250 W for 20-40 minutes; (b) concentrating under reduced pressure, adding 75% to 85% by volume ethanol for alcohol precipitation, standing at 4° C., centrifuging, repeatedly washing the precipitate with anhydrous ethanol, and vacuum freeze-drying to obtain a pueraria polysaccharide extract.

2. The composition of the hypoglycemic plant extract according to claim 1, characterized in that: The weight ratio of mulberry leaf extract, loquat leaf extract, astragalus total saponins, chlorogenic acid and salvianolic acid in the blood sugar lowering plant extract composition is 15: (0.8-1.5): (1.2-1.5): (0.05-0.1): (0.30-0.35).

3. The composition of the hypoglycemic plant extract according to claim 1, characterized in that: The weight ratio of mulberry leaf extract, loquat leaf extract, astragalus total saponins, chlorogenic acid and salvianolic acid in the blood sugar-lowering plant extract composition is 15:(1-1.2):(1.2-1.5):0.08:0.

35.

4. A method for preparing the hypoglycemic plant extract composition according to any one of claims 1 to 3, characterized in that: The method comprises the following steps: (I) drying loquat leaves and grinding them into powder, adding 0.08-0.12 mol / L sodium hydroxide solution in a solid-liquid ratio of 1:(30-50), and ultrasonically extracting at 450-500 W power at 35-42° C. for 20-40 minutes; (II) collecting the supernatant by solid-liquid separation, adjusting the pH to the isoelectric point of 3.5 with hydrochloric acid, allowing to stand for 25 to 35 minutes, collecting the precipitate by centrifugation, washing with distilled water, and freeze-drying to obtain a loquat leaf extract; (III) mixing kudzu root powder and water in a solid-liquid ratio of 1:(30-50), and ultrasonically extracting at 70-85° C. and 150-250 W for 20-40 minutes; (VI) concentrating under reduced pressure, adding 75% to 85% by volume ethanol for alcohol precipitation, standing at 4° C., centrifuging, repeatedly washing the precipitate with anhydrous ethanol, and vacuum freeze-drying to obtain a pueraria polysaccharide extract; (V) Mulberry leaf extract, cinnamon extract, loquat leaf extract, astragalus total saponins, and kudzu root polysaccharide extract are mixed according to a weight ratio, and then vacuum-dried at a temperature below 20° C. for 8 to 15 minutes. Chlorogenic acid and salvianolic acid B are added and mixed to obtain a blood sugar-lowering plant extract composition.

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