Toothpaste for promoting oral mucosal health

Abstract

The present application relates to the technical field of toothpaste, and specifically discloses a toothpaste that promotes the health of oral mucosa, the toothpaste comprising the effective ingredient wild kiwifruit juice extract; the wild kiwifruit juice extract is obtained by mixing wild kiwifruit juice, tea extract and fructose syrup; and also comprises raw materials: moisturizer, abrasive, flavoring agent, foaming agent, thickener, strengthening ingredient, sweetener, solvent; the present application has a simple formula and a strict proportion, and by adding the effective ingredient wild kiwifruit juice extract into the formula, the antioxidants, vitamin C and other bioactive ingredients contained in the wild kiwifruit juice extract have significant anti-inflammatory and antioxidant effects, help relieve gingival swelling and pain, reduce oral inflammation, and promote the repair and health of oral mucosa; the toothpaste prepared by the present application can effectively clean teeth, remove dental plaque and stains, reduce oral bacteria, enhance tooth corrosion resistance, and freshen breath while promoting oral health.

Description

Technical Field

[0001] The present application belongs to the technical field of toothpaste, and more specifically, relates to a toothpaste that promotes the health of oral mucosa. Background Art

[0002] Toothpaste is a daily hygiene product used to clean teeth. Its main function is to remove food residues and dental plaque on the surface of teeth through its detergent ingredients, reduce the risk of tooth decay and periodontal disease, and provide fresh breath. With the development and improvement of toothpaste formula and preparation technology, some toothpastes will also add specific health care functions, such as cleaning, anti-caries, anti-sensitivity, whitening, prevention and treatment of periodontal disease, etc.

[0003] There are many varieties of toothpastes on the current market, and some toothpaste products claim to have multiple health benefits including the prevention and treatment of oral mucosal diseases. These toothpastes usually contain ingredients such as anti-inflammatory, antibacterial or repair aids, such as biological enzymes, chlorhexidine, zinc acetate, etc. These ingredients can help reduce the number of bacteria in the mouth, reduce inflammation, and promote the health of the oral mucosa. Long-term use of toothpaste containing specific drug ingredients is prone to certain side effects. For example, long-term use of toothpaste containing chlorhexidine is likely to destroy the balance of oral microflora, causing discomfort in the oral mucosa, and in severe cases, it can also cause tooth discoloration and change taste perception. Based on the above statement, the present application provides a new toothpaste that promotes the health of the oral mucosa. Summary of the invention

[0004] The present application provides a toothpaste that promotes the health of oral mucosa, using the following technical solution:

[0005] A toothpaste for promoting oral mucosal health comprises a wild kiwifruit juice extract as an effective ingredient; the wild kiwifruit juice extract is obtained by mixing wild kiwifruit juice, tea extract and fructose syrup in a mass ratio of 45-50:1-2:1.

[0006] Preferably, the preparation of the wild kiwifruit juice extract further comprises an enzymatic treatment step after mixing the wild kiwifruit juice, the tea extract and the fructose-glucose syrup.

[0007] Preferably, the wild kiwifruit juice extract is prepared by the following method:

[0008] The wild kiwifruit is washed, peeled, cut into pieces, and placed in a juicer, and water is added to the juicer at a mass ratio of 1:1.5-2, and then the juice is filtered to obtain wild kiwifruit juice;

[0009] The green tea is air-dried and crushed to obtain tea powder, and water is added to the tea powder for ultrasonic extraction at a mass ratio of 1:7-9, followed by centrifugation and concentration to obtain a tea extract;

[0010] The wild kiwifruit juice, tea extract and fructose syrup are mixed in a mass ratio of 45-50:1-2:1 to obtain a premix, and a composite enzyme is added for enzymolysis and filtration to obtain the desired wild kiwifruit juice extract.

[0011] Preferably, the complex enzyme comprises cellulase, pectinase and protease in a mass ratio of 2-4:1-2:1; the addition amount of the complex enzyme is 1-3% of the total mass of the premix, the enzymolysis temperature is 30-50°C, and the enzymolysis time is 1-2h.

[0012] Preferably, the toothpaste raw materials also include at least one of a moisturizer, an abrasive, a flavoring agent, a foaming agent, a thickener, a strengthening ingredient, a sweetener, and a solvent.

[0013] Preferably, the toothpaste for promoting oral mucosal health comprises the following raw materials in parts by weight: 60-70 parts of moisturizer, 10-20 parts of abrasive, 2-10 parts of flavoring agent, 1-3 parts of foaming agent, 0.1-1 part of thickener, 0.8-2 parts of effective ingredient, 0.1-1 part of strengthening ingredient, 0.1-1 part of sweetener, and 5-20 parts of solvent.

[0014] Preferably, the humectant is at least one of sorbitol, polyethylene glycol, propylene glycol and glycerol.

[0015] Preferably, the friction agent is at least one of hydrated silica, silicon dioxide, calcium carbonate and calcium bicarbonate.

[0016] Preferably, the flavoring agent is mint and / or zinc citrate.

[0017] Preferably, the flavoring agent is a compound of mint and zinc citrate in a mass ratio of 5-7:1.

[0018] Preferably, the foaming agent is cocamidopropyl betaine and / or sodium lauryl sulfate.

[0019] Preferably, the thickener is at least one of sodium carboxymethyl cellulose, hydroxyethyl cellulose, xanthan gum and carrageenan.

[0020] Preferably, the strengthening component is sodium fluoride and / or hydroxyapatite.

[0021] Preferably, the strengthening component is a compound of sodium fluoride and hydroxyapatite in a mass ratio of 1-1.2:1.

[0022] Preferably, the sweetener is sucralose and / or saccharin sodium.

[0023] The solvent is deionized water.

[0024] Preferably, the toothpaste for promoting oral mucosal health is prepared by the following method:

[0025] S1. Premixing the aqueous phase: adding the sweetener, foaming agent and functional ingredients in the formula to the solvent in parts by weight, stirring and mixing evenly to obtain the aqueous phase;

[0026] S2. Premixing of powder: Mix the thickener, flavoring agent, abrasive, and strengthening ingredients in the formula evenly to obtain powder;

[0027] S3. Premix the moisturizer with the water phase, stir to deaerate, then add the powder to premix under vacuum, stir to deaerate under vacuum until there are no obvious bubbles, and then discharge and fill.

[0028] In summary, this application has the following beneficial effects:

[0029] The formula of the present application is simple and the ratio is rigorous. By adding the effective ingredient wild kiwi juice extract to the formula, the antioxidants, vitamin C and other bioactive ingredients contained in it have significant anti-inflammatory and antioxidant effects, which can help relieve gingival swelling and pain, reduce oral inflammation, and promote the repair and health of oral mucosa; the toothpaste prepared by the present application can effectively clean teeth, remove dental plaque and stains, reduce oral bacteria, enhance tooth corrosion resistance, and freshen breath while promoting oral health.

[0030] The active ingredient wild kiwi juice extract of the present application is obtained by enzymatic hydrolysis of wild kiwi juice, tea extract and fructose syrup after pre-mixing. By adding a small amount of tea extract and fructose syrup to the wild kiwi juice, the sweetness of the active ingredients can be increased, the taste can be enriched, the anti-inflammatory and antioxidant capabilities can be enhanced, and the stability of the kiwi juice can be improved; by pre-mixing, the uniformity of each ingredient added to the final product can be ensured, and the quality and consistency of the product can be improved; the enzymatic hydrolysis process can help release and activate the active substances in the natural ingredients, enhance the stability and efficacy of the active ingredients, and the mixed enzymatic hydrolysis can effectively ensure the interaction between the components of the mixture, further improving the release or stability of the active ingredients. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 The figures are the states of the oral mucosa of the rats in the blank control group and the Example 1 group of the present application; wherein A is the state of the oral mucosa of normal rats; B is the state of the oral mucosa of the rats in the Example 1 group 24 hours after burning.

[0032] Figure 2 This is a general morphological diagram of oral ulcers in rats in Example 1 of the present application from onset to healing.

[0033] Figure 3 This is the gross morphological diagram of oral ulcers in 5 groups of rats in the comparative example of this application from occurrence to healing.

[0034] Figure 4This is the gross morphology of oral ulcers in rats in the model group of this application from occurrence to healing.

[0035] Figure 5 This is a scraping and staining observation picture of the oral mucosa of rats in the blank control group of this application.

[0036] Figure 6 This is a picture of the oral mucosa of rats in Example 1 of the present application.

[0037] Figure 7 This is a scraping and staining observation picture of the oral mucosa of rats in group 5 of the comparative example of this application.

[0038] Figure 8 This is a scraping and staining observation picture of the oral mucosa of rats in the model group of this application. DETAILED DESCRIPTION

[0039] The present application is further described in detail below in conjunction with preparation examples and embodiments.

[0040] Preparation Examples 1-3 and Comparative Preparation Examples 1-5 provide a wild kiwifruit juice extract.

[0041] Preparation Example 1

[0042] Wild kiwifruit juice extract is prepared by the following method:

[0043] Wash, peel and cut fresh wild kiwifruit from Nanyang into pieces, put them into a juicer, add water to make pulp at a mass ratio of 1:1.5, and filter with 300-mesh gauze to obtain wild kiwifruit juice;

[0044] Fresh Rizhao green tea was air-dried at 50°C and then crushed to pass through a 100-mesh sieve. The tea powder was added with deionized water at a mass ratio of 1:7 and ultrasonically extracted for 3 minutes. The ultrasonic temperature was controlled to be 50°C, the ultrasonic power was 340W, and the ultrasonic frequency was 36kHz. After the ultrasonication was completed, the mixture was centrifuged at 5000rpm for 4 minutes. The centrifuged liquid was concentrated in a rotary evaporator at 50°C to a solid content of 30%, thereby obtaining a tea extract.

[0045] Wild kiwi juice, tea extract and fructose syrup were mixed evenly in a mass ratio of 45:1:1 to obtain a premix, and 1% of the total mass of the premix was added with a composite enzyme for enzymolysis for 2 hours. The enzymolysis temperature was controlled at 50°C, and after the enzymolysis was completed, the enzyme was inactivated at 115°C for 1 minute and filtered through a 1μm filter membrane to obtain the required wild kiwi juice extract; wherein the composite enzyme was cellulase (model FDG-2225, brand Xiasheng), pectinase (model FDG-2259, brand Xiasheng) and acid protease (model FDY-2205, brand Xiasheng) in a mass ratio of 2:1:1.

[0046] Preparation Example 2

[0047] Wild kiwifruit juice extract is prepared by the following method:

[0048] Wash, peel and cut fresh wild kiwifruit from Nanyang into pieces, put them into a juicer, add water to make pulp at a mass ratio of 1:1.8, and filter with 300-mesh gauze to obtain wild kiwifruit juice;

[0049] Fresh Rizhao green tea was air-dried at 50°C and then crushed to pass through a 100-mesh sieve. The tea powder was added with deionized water at a mass ratio of 1:8 and ultrasonically extracted for 4 minutes. The ultrasonic temperature was controlled at 60°C, the ultrasonic power was 320W, and the ultrasonic frequency was 32kHz. After the ultrasonication was completed, the mixture was centrifuged at 5000rpm for 4 minutes. The centrifuged liquid was concentrated in a rotary evaporator at 50°C to a solid content of 30%, thereby obtaining a tea extract.

[0050] Wild kiwi juice, tea extract and fructose syrup were mixed evenly in a mass ratio of 48:1.5:1 to obtain a premix, and 2% of the total mass of the premix was added with a composite enzyme for enzymolysis for 1.5 h. The enzymolysis temperature was controlled at 40°C. After the enzymolysis was completed, the enzyme was inactivated at 115°C for 1 min, and the mixture was filtered through a 5μm filter membrane to obtain the required wild kiwi juice extract; wherein the composite enzyme was cellulase (model FDG-2225, brand Xiasheng), pectinase (model FDG-2259, brand Xiasheng) and acid protease (model FDY-2205, brand Xiasheng) in a mass ratio of 3:1.5:1.

[0051] Preparation Example 3

[0052] Wild kiwifruit juice extract is prepared by the following method:

[0053] Wash, peel and cut fresh wild kiwifruit from Nanyang into pieces, put them into a juicer, add water to make pulp in a mass ratio of 1:2, and filter with 300-mesh gauze to obtain wild kiwifruit juice;

[0054] Fresh Rizhao green tea was air-dried at 50°C and then crushed to pass through a 100-mesh sieve. The tea powder was added with deionized water at a mass ratio of 1:9 and ultrasonically extracted for 5 minutes. The ultrasonic temperature was controlled to be 70°C, the ultrasonic power was 300W, and the ultrasonic frequency was 28kHz. After the ultrasonication was completed, the mixture was centrifuged at 5000rpm for 4 minutes. The centrifuged liquid was concentrated in a rotary evaporator at 50°C to a solid content of 30%, thereby obtaining a tea extract.

[0055] Wild kiwi juice, tea extract and fructose syrup were mixed evenly in a mass ratio of 50:2:1 to obtain a premix, and 3% of the total mass of the premix was added with a composite enzyme for enzymolysis for 1 hour. The enzymolysis temperature was controlled at 30°C, and after the enzymolysis was completed, the enzyme was inactivated at 115°C for 1 minute, and the mixture was filtered through a 10μm filter membrane to obtain the required wild kiwi juice extract; wherein the composite enzyme was cellulase (model FDG-2225, brand Xiasheng), pectinase (model FDG-2259, brand Xiasheng) and acid protease (model FDY-2205, brand Xiasheng) in a mass ratio of 4:2:1.

[0056] Comparative Preparation Example 1

[0057] Wild kiwifruit juice extract is prepared by the following method:

[0058] Wash, peel and cut fresh wild kiwifruit from Nanyang into pieces, put them into a juicer, add water to make pulp at a mass ratio of 1:1.5, and filter with 300-mesh gauze to obtain wild kiwifruit juice;

[0059] Fresh Rizhao green tea was air-dried at 50°C and then crushed to pass through a 100-mesh sieve. The tea powder was added with deionized water at a mass ratio of 1:7 and ultrasonically extracted for 3 minutes. The ultrasonic temperature was controlled to be 50°C, the ultrasonic power was 340W, and the ultrasonic frequency was 36kHz. After the ultrasonication was completed, the mixture was centrifuged at 5000rpm for 4 minutes. The centrifuged liquid was concentrated in a rotary evaporator at 50°C to a solid content of 30%, thereby obtaining a tea extract.

[0060] The wild kiwifruit juice, tea extract and fructose syrup were uniformly mixed in a mass ratio of 45:1:1, and then filtered through a 1 μm filter membrane to obtain the desired wild kiwifruit juice extract.

[0061] Comparative Preparation Example 2

[0062] Wild kiwifruit juice extract is prepared by the following method:

[0063] Wash, peel and cut fresh wild kiwifruit from Nanyang into pieces, put them into a juicer, add water to make pulp at a mass ratio of 1:1.5, and filter with 300-mesh gauze to obtain wild kiwifruit juice;

[0064] Fresh Rizhao green tea was air-dried at 50°C and then crushed to pass through a 100-mesh sieve. The tea powder was added with deionized water at a mass ratio of 1:7 and ultrasonically extracted for 3 minutes. The ultrasonic temperature was controlled to be 50°C, the ultrasonic power was 340W, and the ultrasonic frequency was 36kHz. After the ultrasonication was completed, the mixture was centrifuged at 5000rpm for 4 minutes. The centrifuged liquid was concentrated in a rotary evaporator at 50°C to a solid content of 30%, thereby obtaining a tea extract.

[0065] Wild kiwi juice and tea extract were uniformly mixed at a mass ratio of 45:2 to obtain a premix, and 1% of the total mass of the premix was added with a composite enzyme for enzymolysis for 2 hours. The enzymolysis temperature was controlled at 50°C, and after the enzymolysis was completed, the enzyme was inactivated at 115°C for 1 minute, and the extract was filtered through a 1μm filter membrane to obtain the required wild kiwi juice extract; wherein the composite enzyme was cellulase (model FDG-2225, brand Xiasheng), pectinase (model FDG-2259, brand Xiasheng) and acid protease (model FDY-2205, brand Xiasheng) in a mass ratio of 2:1:1.

[0066] Comparative Preparation Example 3

[0067] Wild kiwifruit juice extract is prepared by the following method:

[0068] Wash, peel and cut fresh Nanyang wild kiwifruit into pieces, put them into a juicer, add water to make pulp at a mass ratio of 1:1.5, and filter with 300-mesh gauze to obtain wild kiwifruit juice;

[0069] Fresh Rizhao green tea was air-dried at 50°C and then crushed to pass through a 100-mesh sieve. The tea powder was added with deionized water at a mass ratio of 1:7 and ultrasonically extracted for 3 minutes. The ultrasonic temperature was controlled to be 50°C, the ultrasonic power was 340W, and the ultrasonic frequency was 36kHz. After the ultrasonication was completed, the mixture was centrifuged at 5000rpm for 4 minutes. The centrifuged liquid was concentrated in a rotary evaporator at 50°C to a solid content of 30%, thereby obtaining a tea extract.

[0070] Wild kiwi juice and fructose syrup were uniformly mixed in a mass ratio of 45:2 to obtain a premix, and 1% of the total mass of the premix was added with a composite enzyme for enzymolysis for 2 hours. The enzymolysis temperature was controlled at 50°C, and after the enzymolysis was completed, the enzyme was inactivated at 115°C for 1 minute, and the extract was filtered through a 1μm filter membrane to obtain the required wild kiwi juice extract; wherein the composite enzyme was cellulase (model FDG-2225, brand Xiasheng), pectinase (model FDG-2259, brand Xiasheng) and acid protease (model FDY-2205, brand Xiasheng) in a mass ratio of 2:1:1.

[0071] Comparative Preparation Example 4

[0072] Wild kiwifruit juice extract is prepared by the following method:

[0073] Wash, peel and cut fresh Nanyang wild kiwifruit into pieces, put them into a juicer, add water to make pulp at a mass ratio of 1:1.5, and filter with 300-mesh gauze to obtain wild kiwifruit juice;

[0074] Add 1% of the total weight of composite enzyme to the wild kiwifruit juice for enzymolysis for 2 hours, control the enzymolysis temperature at 50°C, inactivate the enzyme at 115°C for 1 minute, and filter through a 1μm filter membrane to obtain the required wild kiwifruit juice extract; wherein the composite enzyme is cellulase (model FDG-2225, brand Xiasheng), pectinase (model FDG-2259, brand Xiasheng) and acid protease (model FDY-2205, brand Xiasheng) in a mass ratio of 2:1:1.

[0075] Comparative Preparation Example 5

[0076] The kiwifruit juice extract is prepared by the following method:

[0077] Wash, peel and cut fresh Xuxiang kiwifruit into pieces, put them into a juicer, add water to make pulp at a mass ratio of 1:1.5, and filter with 300-mesh gauze to obtain kiwifruit juice;

[0078] Fresh Rizhao green tea was air-dried at 50°C and then crushed to pass through a 100-mesh sieve. The tea powder was added with deionized water at a mass ratio of 1:7 and ultrasonically extracted for 3 minutes. The ultrasonic temperature was controlled at 50°C, the ultrasonic power was 340W, and the ultrasonic frequency was 36kHz. After the ultrasonication was completed, the mixture was centrifuged at 5000rpm for 4 minutes. The centrifuged liquid was concentrated in a rotary evaporator at 50°C to a solid content of 30%, thereby obtaining a tea extract.

[0079] Kiwi juice, tea extract and fructose syrup were mixed evenly in a mass ratio of 45:1:1 to obtain a premix, and 1% of the total mass of the premix was added with a composite enzyme for enzymolysis for 2 hours. The enzymolysis temperature was controlled at 50°C, and after the enzymolysis was completed, the enzyme was inactivated at 115°C for 1 minute, and filtered through a 1μm filter membrane to obtain the required kiwi juice extract; wherein the composite enzyme was cellulase (model FDG-2225, brand Xiasheng), pectinase (model FDG-2259, brand Xiasheng) and acid protease (model FDY-2205, brand Xiasheng) in a mass ratio of 2:1:1.

[0080] Embodiments 1-3 provide a toothpaste that promotes the health of oral mucosa.

[0081] Example 1

[0082] A toothpaste for promoting oral mucosal health, comprising the following raw materials in parts by weight: 60 parts of sorbitol, 10 parts of hydrated silica, 2 parts of flavoring agent, 1 part of cocamidopropyl betaine, 0.1 part of sodium carboxymethyl cellulose, 0.8 parts of effective ingredients, 0.1 parts of strengthening ingredients, 0.1 parts of sucralose, and 5 parts of deionized water;

[0083] The flavoring agent is a compound of mint and zinc citrate in a mass ratio of 5:1;

[0084] The active ingredient is the wild kiwifruit juice extract in Preparation Example 1;

[0085] The strengthening component is a compound of sodium fluoride and hydroxyapatite in a mass ratio of 1:1.

[0086] A toothpaste for promoting oral mucosal health is prepared by the following method:

[0087] S1. Premixing of aqueous phase: Add sucralose, cocamidopropyl betaine and functional ingredients in the formula to deionized water in order by weight, control the stirring speed to 600 rpm, stir at room temperature for 12 minutes to mix evenly, and obtain aqueous phase;

[0088] S2. Premixing of powders: Mix the sodium carboxymethyl cellulose, flavoring agent, hydrated silica and strengthening ingredients in the formula at a stirring speed of 300 rpm and stir at room temperature for 20 minutes to obtain powders;

[0089] S3. Control the stirring speed to 800 rpm, premix sorbitol with water phase, stir at room temperature for 8 minutes to deaerate, then adjust the vacuum degree to -0.085 MPa, control the stirring speed to 2500 rpm, add powder premix, vacuum stir and deaerate until the vacuum degree reaches -0.095 MPa, continue to stir for 15 minutes to deaerate until there are no obvious bubbles, and then discharge and fill.

[0090] Example 2

[0091] A toothpaste for promoting oral mucosal health, comprising the following raw materials in parts by weight: 65 parts of sorbitol, 12 parts of hydrated silica, 8.8 parts of flavoring agent, 3 parts of cocamidopropyl betaine, 1 part of sodium carboxymethyl cellulose, 0.9 parts of effective ingredients, 0.44 parts of strengthening ingredients, 0.15 parts of sucralose, and 6.6 parts of deionized water;

[0092] The flavoring agent is a compound of mint and zinc citrate in a mass ratio of 6:1;

[0093] The active ingredient is the wild kiwifruit juice extract in Preparation Example 2;

[0094] The strengthening component is a compound of sodium fluoride and hydroxyapatite in a mass ratio of 1.1:1.

[0095] A toothpaste for promoting oral mucosal health is prepared by the following method:

[0096] S1. Premixing of aqueous phase: Add sucralose, cocamidopropyl betaine and functional ingredients in the formula to deionized water in order by weight, control the stirring speed to 700 rpm, stir at room temperature for 9 minutes to mix evenly, and obtain an aqueous phase;

[0097] S2. Premixing of powders: Mix the sodium carboxymethyl cellulose, flavoring agent, hydrated silica and strengthening ingredients in the formula at a stirring speed of 400 rpm and stir at room temperature for 16 minutes to obtain powders;

[0098] S3. Control the stirring speed to 900 rpm, premix sorbitol with water phase, stir at room temperature for 6 minutes to deaerate, then adjust the vacuum degree to -0.085 MPa, control the stirring speed to 3000 rpm, add powder premix, vacuum stir and deaerate until the vacuum degree reaches -0.095 MPa, continue to stir for 12 minutes to deaerate until there are no obvious bubbles, and then discharge and fill.

[0099] Example 3

[0100] A toothpaste for promoting oral mucosal health, comprising the following raw materials in parts by weight: 70 parts of sorbitol, 20 parts of hydrated silica, 10 parts of flavoring agent, 3 parts of cocamidopropyl betaine, 1 part of sodium carboxymethyl cellulose, 2 parts of effective ingredients, 1 part of strengthening ingredients, 1 part of sucralose, and 20 parts of deionized water;

[0101] The flavoring agent is a compound of mint and zinc citrate in a mass ratio of 7:1;

[0102] The active ingredient is the wild kiwifruit juice extract in Preparation Example 3;

[0103] The strengthening component is a compound of sodium fluoride and hydroxyapatite in a mass ratio of 1.2:1.

[0104] A toothpaste for promoting the health of oral mucosa is prepared by the following method:

[0105] S1. Premixing of aqueous phase: adding sucralose, cocamidopropyl betaine and functional ingredients in the formula to deionized water in parts by weight, controlling the stirring speed to 800 rpm, stirring at room temperature for 6 minutes to mix evenly, to obtain an aqueous phase;

[0106] S2. Premixing of powders: Mix the sodium carboxymethyl cellulose, flavoring agent, hydrated silica and strengthening ingredients in the formula at a stirring speed of 500 rpm and stir at room temperature for 12 minutes to obtain powders;

[0107] S3. Control the stirring speed to 1000rpm, premix sorbitol with water phase, stir at room temperature for 4min to deaerate, then adjust the vacuum degree to -0.085MPa, control the stirring speed to 3500rpm, add powder premix, vacuum stir and deaerate until the vacuum degree reaches -0.095MPa, continue stirring for 10min to deaerate until there are no obvious bubbles, then discharge and fill.

[0108] In order to verify the comprehensive effect of the toothpaste prepared in Examples 1-3 of the present application on promoting the health of oral mucosa, the applicant set up comparative examples 1-5, which are as follows:

[0109] A toothpaste comprising the following raw materials in parts by weight: 60 parts of sorbitol, 10 parts of hydrated silica, 2 parts of flavoring agent, 1 part of cocamidopropyl betaine, 0.1 part of sodium carboxymethyl cellulose, 0.8 part of functional ingredient, 0.1 part of strengthening ingredient, 0.1 part of sucralose, and 5 parts of deionized water;

[0110] The flavoring agent is a compound of mint and zinc citrate in a mass ratio of 5:1;

[0111] The active ingredient is the wild kiwifruit juice extract in Comparative Preparation Example 1;

[0112] The strengthening component is a compound of sodium fluoride and hydroxyapatite in a mass ratio of 1:1.

[0113] The toothpaste is prepared by the following method:

[0114] S1. Premixing of aqueous phase: Add sucralose, cocamidopropyl betaine and functional ingredients in the formula to deionized water in order by weight, control the stirring speed to 600 rpm, stir at room temperature for 12 minutes to mix evenly, and obtain aqueous phase;

[0115] S2. Premixing of powders: Mix the sodium carboxymethyl cellulose, flavoring agent, hydrated silica and strengthening ingredients in the formula at a stirring speed of 300 rpm and stir at room temperature for 20 minutes to obtain powders;

[0116] S3. Control the stirring speed to 800 rpm, premix sorbitol with water phase, stir at room temperature for 8 minutes to deaerate, then adjust the vacuum degree to -0.085 MPa, control the stirring speed to 2500 rpm, add powder premix, vacuum stir and deaerate until the vacuum degree reaches -0.095 MPa, continue to stir for 15 minutes to deaerate until there are no obvious bubbles, and then discharge and fill.

[0117] Comparative Example 2

[0118] A toothpaste comprising the following raw materials in parts by weight: 60 parts of sorbitol, 10 parts of hydrated silica, 2 parts of flavoring agent, 1 part of cocamidopropyl betaine, 0.1 part of sodium carboxymethyl cellulose, 0.8 part of functional ingredient, 0.1 part of strengthening ingredient, 0.1 part of sucralose, and 5 parts of deionized water;

[0119] The flavoring agent is a compound of mint and zinc citrate in a mass ratio of 5:1;

[0120] The active ingredient is the wild kiwifruit juice extract in Comparative Preparation Example 2;

[0121] The strengthening component is a compound of sodium fluoride and hydroxyapatite in a mass ratio of 1:1.

[0122] The toothpaste is prepared by the following method:

[0123] S1. Premixing of aqueous phase: Add sucralose, cocamidopropyl betaine and functional ingredients in the formula to deionized water in order by weight, control the stirring speed to 600 rpm, stir at room temperature for 12 minutes to mix evenly, and obtain aqueous phase;

[0124] S2. Premixing of powders: Mix the sodium carboxymethyl cellulose, flavoring agent, hydrated silica and strengthening ingredients in the formula at a stirring speed of 300 rpm and stir at room temperature for 20 minutes to obtain powders;

[0125] S3. Control the stirring speed to 800 rpm, premix sorbitol with water phase, stir at room temperature for 8 minutes to deaerate, then adjust the vacuum degree to -0.085 MPa, control the stirring speed to 2500 rpm, add powder premix, vacuum stir and deaerate until the vacuum degree reaches -0.095 MPa, continue to stir for 15 minutes to deaerate until there are no obvious bubbles, and then discharge and fill.

[0126] Comparative Example 3

[0127] A toothpaste comprising the following raw materials in parts by weight: 60 parts of sorbitol, 10 parts of hydrated silica, 2 parts of flavoring agent, 1 part of cocamidopropyl betaine, 0.1 part of sodium carboxymethyl cellulose, 0.8 part of functional ingredient, 0.1 part of strengthening ingredient, 0.1 part of sucralose, and 5 parts of deionized water;

[0128] The flavoring agent is a compound of mint and zinc citrate in a mass ratio of 5:1;

[0129] The active ingredient is the wild kiwifruit juice extract in Comparative Preparation Example 3;

[0130] The strengthening component is a compound of sodium fluoride and hydroxyapatite in a mass ratio of 1:1.

[0131] The toothpaste is prepared by the following method:

[0132] S1. Premixing of aqueous phase: Add sucralose, cocamidopropyl betaine and functional ingredients in the formula to deionized water in order by weight, control the stirring speed to 600 rpm, stir at room temperature for 12 minutes to mix evenly, and obtain aqueous phase;

[0133] S2. Premixing of powders: Mix the sodium carboxymethyl cellulose, flavoring agent, hydrated silica and strengthening ingredients in the formula at a stirring speed of 300 rpm and stir at room temperature for 20 minutes to obtain powders;

[0134] S3. Control the stirring speed to 800 rpm, premix sorbitol with water phase, stir at room temperature for 8 minutes to deaerate, then adjust the vacuum degree to -0.085 MPa, control the stirring speed to 2500 rpm, add powder premix, vacuum stir and deaerate until the vacuum degree reaches -0.095 MPa, continue to stir for 15 minutes to deaerate until there are no obvious bubbles, and then discharge and fill.

[0135] Comparative Example 4

[0136] A toothpaste comprising the following raw materials in parts by weight: 60 parts of sorbitol, 10 parts of hydrated silica, 2 parts of flavoring agent, 1 part of cocamidopropyl betaine, 0.1 part of sodium carboxymethyl cellulose, 0.8 part of functional ingredient, 0.1 part of strengthening ingredient, 0.1 part of sucralose, and 5 parts of deionized water;

[0137] The flavoring agent is a compound of mint and zinc citrate in a mass ratio of 5:1;

[0138] The active ingredient is the wild kiwifruit juice extract in Comparative Preparation Example 4;

[0139] The strengthening component is a compound of sodium fluoride and hydroxyapatite in a mass ratio of 1:1.

[0140] The toothpaste is prepared by the following method:

[0141] S1. Premixing of aqueous phase: Add sucralose, cocamidopropyl betaine and functional ingredients in the formula to deionized water in order by weight, control the stirring speed to 600 rpm, stir at room temperature for 12 minutes to mix evenly, and obtain aqueous phase;

[0142] S2. Premixing of powders: Mix the sodium carboxymethyl cellulose, flavoring agent, hydrated silica and strengthening ingredients in the formula at a stirring speed of 300 rpm and stir at room temperature for 20 minutes to obtain powders;

[0143] S3. Control the stirring speed to 800 rpm, premix sorbitol with water phase, stir at room temperature for 8 minutes to deaerate, then adjust the vacuum degree to -0.085 MPa, control the stirring speed to 2500 rpm, add powder premix, vacuum stir and deaerate until the vacuum degree reaches -0.095 MPa, continue to stir for 15 minutes to deaerate until there are no obvious bubbles, and then discharge and fill.

[0144] Comparative Example 5

[0145] A toothpaste comprising the following raw materials in parts by weight: 60 parts of sorbitol, 10 parts of hydrated silica, 2 parts of flavoring agent, 1 part of cocamidopropyl betaine, 0.1 part of sodium carboxymethyl cellulose, 0.8 part of functional ingredient, 0.1 part of strengthening ingredient, 0.1 part of sucralose, and 5 parts of deionized water;

[0146] The flavoring agent is a compound of mint and zinc citrate in a mass ratio of 5:1;

[0147] The active ingredient is the kiwifruit juice extract in Comparative Preparation Example 5;

[0148] The strengthening component is a compound of sodium fluoride and hydroxyapatite in a mass ratio of 1:1.

[0149] The toothpaste is prepared by the following method:

[0150] S1. Premixing of aqueous phase: Add sucralose, cocamidopropyl betaine and functional ingredients in the formula to deionized water in order by weight, control the stirring speed to 600 rpm, stir at room temperature for 12 minutes to mix evenly, and obtain aqueous phase;

[0151] S2. Premixing of powders: Mix the sodium carboxymethyl cellulose, flavoring agent, hydrated silica and strengthening ingredients in the formula at a stirring speed of 300 rpm and stir at room temperature for 20 minutes to obtain powders;

[0152] S3. Control the stirring speed to 800 rpm, premix sorbitol with water phase, stir at room temperature for 8 minutes to deaerate, then adjust the vacuum degree to -0.085 MPa, control the stirring speed to 2500 rpm, add powder premix, vacuum stir and deaerate until the vacuum degree reaches -0.095 MPa, continue to stir for 15 minutes to deaerate until there are no obvious bubbles, and then discharge and fill.

[0153] Performance Testing

[0154] The therapeutic effects of the toothpastes prepared in Examples 1-3 of the present application and Comparative Examples 1-5 on oral ulcers were tested respectively.

[0155] 1. Oral ulcer healing experiment

[0156] Experimental content: A total of 30 clean-grade SD rats (weighing 250-300 g) were randomly divided into 10 groups, with 3 rats in each group, and were named as Example 1-3 and Comparative Example 1-5 groups, a model group, and a blank control group.

[0157] Before modeling, the rats were fed and watered freely and were adaptively fed for one week. The rats in Example 1-3, Comparative Example 1-5 and Model Group were subjected to chemical burning modeling on the buccal mucosa of rats with glacial acetic acid. Specifically, the rats were anesthetized by intraperitoneal injection of sodium pentobarbital (concentration 1%, 0.5mL / 100g), and then the oral cavity of the rats was opened with cotton thread, and a fixed-size mold was made using a 200-microliter pipette tip, and a small amount of cotton balls were placed in the mold. 15 microliters of 80% glacial acetic acid was absorbed, and the cotton balls were placed on the buccal mucosa of the anesthetized rats for 30s (chemical burning of the epithelial mucosal layer) after absorption. The residual glacial acetic acid was then removed, and the immediate reaction of the treated site was recorded. The tail was numbered, and the rats were fed normally for 24 hours, and then the ulcer modeling of the oral mucosa of the rats was observed. The blank control group was not modeled. Compared with the blank control group, the oral ulcer wounds of the other groups were obvious, and the ulcer surface was covered with a yellow-white pseudomembrane, that is, the model was successfully established. Figure 1 Figure 1 is a diagram showing the oral mucosal status of rats in the blank control group and Example 1 group; wherein A is the oral mucosal status of normal rats; B is the oral mucosal status of rats in Example 1 group 24 hours after burning.

[0158] The modeling rats were treated 24 hours after the modeling was completed. The rats in Example 1-3 and Comparative Example 1-5 groups were treated with toothpaste (0.05 g) for promoting oral mucosal health prepared in Example 1-3 and Comparative Example 1-5 of the present application, respectively. The model group was treated with saline paste twice a day, each lasting for 3 minutes and then wiped off. The treatment interval was 6 hours. The gross morphology of the ulcer from occurrence to healing was regularly observed and recorded every day. The treatment was continued until the ulcer was completely healed as the end point. The average healing days of oral ulcers are shown in Table 1 below.

[0159] Table 1:

[0160]

[0161] The data shown in Table 1 above are combined Figure 2-4 It can be seen that the ulcer surface healing of the toothpaste for promoting oral mucosal health prepared in Examples 1-3 is significantly better than that of Comparative Examples 1-5 and the model group, and the effect of promoting oral mucosal health is significant and worthy of promotion.

[0162] 2. Rat oral mucosa scraping staining observation experiment

[0163] Experimental content: 1. Rinse the rat's oral cavity with saline to remove food residues and other impurities in the oral cavity; 2. Take a clean glass slide, use the wide end of a sterilized toothpick to scrape epithelial cells from the wound of the buccal mucosa of the rats in the blank control group, Example 1 group, Comparative Example 5 group and model group, drop a drop of saline in the center of the glass slide with a dropper, and apply the scraped epithelial cells to the saline in the center of the glass slide; 3. Wait for the saline to dry naturally to fix the epithelial cells; 4. Use the hematoxylin-eosin staining kit to stain; 5. Add a drop of resin on the glass slide, cover it with a cover glass, and then observe it under an electron microscope. The results are shown in Figure 5-8 .

[0164] Depend on Figure 5-8 It can be seen from the display that: to ensure the representativeness of the stained sections, two pictures were retained for each group (both were taken 5 days after administration). From the results of the stained smears scraped from the mucosa, it can be seen that there were no shed cell nuclei in the blank control group, a large number of shed cell nuclei were present in the smears of the model group, a small number of shed cell nuclei were present in the Example 1 group and the Comparative Example 5 group, and the shed cell nuclei were the least in the mucosal smears of the Example 1 group. The above results show that the treatment of the Example 1 group and the Comparative Example 5 group is effective, and combined with Table 1 and Figure 2-3 It can be further seen that the treatment effect of Example 1 group is significantly better than that of Comparative Example 5 group. Specifically, the ulcer area before treatment in Example 1 was 6 6mm 2 , Comparative Example 5 Ulcer area before treatment 5 5 mm 2 , and the healing days of Example 1 were shortened by 1 day compared with Comparative Example 5.

[0165] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A toothpaste for promoting oral mucosal health, characterized in that: The invention comprises a wild kiwifruit juice extract as an effective ingredient; the wild kiwifruit juice extract is obtained by mixing wild kiwifruit juice, tea extract and fructose syrup in a mass ratio of 45-50:1-2:1; The wild kiwifruit juice extract is prepared by the following method: Wash, peel and cut fresh Nanyang wild kiwifruit into pieces, put them into a juicer, add water to pulp at a mass ratio of 1:1.5-2, and filter to obtain wild kiwifruit juice; The green tea is air-dried and crushed to obtain tea powder, and water is added to the tea powder for ultrasonic extraction at a mass ratio of 1:7-9, followed by centrifugation and concentration to obtain a tea extract; wild kiwifruit juice, tea extract and fructose syrup are mixed in a mass ratio of 45-50:1-2:1 to obtain a premix, and then a composite enzyme is added for enzymolysis and filtration to obtain the desired wild kiwifruit juice extract; The complex enzyme comprises cellulase, pectinase and acid protease in a mass ratio of 2-4:1-2:1; the addition amount of the complex enzyme is 1-3% of the total mass of the premix, the enzymolysis temperature is 30-50°C, and the enzymolysis time is 1-2h.

2. The toothpaste for promoting oral mucosal health according to claim 1, characterized in that: The raw materials of the toothpaste also include at least one of a moisturizer, an abrasive, a flavoring agent, a foaming agent, a thickener, a strengthening component, a sweetener, and a solvent.

3. The toothpaste for promoting oral mucosal health according to claim 2, characterized in that: The toothpaste comprises the following raw materials in parts by weight: 60-70 parts of moisturizing agent, 10-20 parts of abrasive, 2-10 parts of flavoring agent, 1-3 parts of foaming agent, 0.1-1 part of thickener, 0.8-2 parts of functional ingredient, 0.1-1 part of strengthening ingredient, 0.1-1 part of sweetener and 5-20 parts of solvent.

4. The toothpaste for promoting oral mucosal health according to claim 3, characterized in that: The moisturizing agent is at least one of sorbitol, polyethylene glycol, propylene glycol, and glycerin; the abrasive is at least one of hydrated silica, silicon dioxide, calcium carbonate, and calcium bicarbonate; the flavoring agent is mint and / or zinc citrate; the foaming agent is cocamidopropyl betaine and / or sodium lauryl sulfate; the thickener is at least one of sodium carboxymethyl cellulose, hydroxyethyl cellulose, xanthan gum, and carrageenan; the strengthening ingredient is sodium fluoride and / or hydroxyapatite; the sweetener is sucralose and / or sodium saccharin; and the solvent is deionized water.

5. The toothpaste for promoting oral mucosal health according to claim 4, characterized in that: The flavoring agent is a compound of mint and zinc citrate in a mass ratio of 5-7:

1.

6. The toothpaste for promoting oral mucosal health according to claim 4, characterized in that: The strengthening component is a compound of sodium fluoride and hydroxyapatite in a mass ratio of 1-1.2:

1.

7. The toothpaste for promoting oral mucosal health according to claim 3, characterized in that: The toothpaste is prepared by the following method: S1. Premixing the aqueous phase: adding the sweetener, foaming agent and functional ingredients in the formula to the solvent in order by weight, stirring and mixing evenly to obtain the aqueous phase; S2. Premixing of powder: Mix the thickener, flavoring agent, abrasive, and strengthening ingredients in the formula evenly to obtain powder; S3. Premix the moisturizer with the water phase, stir to deaerate, then add the powder to premix under vacuum, stir to deaerate under vacuum until there are no obvious bubbles, and then discharge and fill.

Images