A cell culture reactor

Abstract

The present invention provides a cell culture reactor, which belongs to the field of cell culture technology, and includes a box, a reaction mechanism and a vibration mechanism. The top of the box is provided with a first cavity, and the bottom of the box is provided with a second cavity; the reaction mechanism includes a fixed seat fixedly installed on the bottom wall of the first cavity, the internal movably connected to a lifting seat of the fixed seat, the top of the lifting seat is threadedly connected to a reaction tank, the upper end of the reaction tank is open, a cover plate is provided on the open, and an air inlet pipe is provided on the left side of the cover plate. Through the vibration mechanism, the reaction tank containing cell fluid can be driven to vibrate up and down, so that the culture fluid and cells can be effectively mixed, the uniform distribution of nutrients and oxygen is promoted, and the growth rate and yield of cells are significantly improved. At the same time, the shear force generated by the vibration method is small, and compared with the traditional mechanical stirring, the damage to cells is reduced, especially for suspended cells and sensitive cells.

Description

Technical Field

[0001] The invention relates to the technical field of cell culture, in particular to a cell culture reactor. Background Art

[0002] Cell culture refers to the process of making cells from tissues, organs or organisms proliferate and propagate under artificial conditions in vitro in order to obtain enough cells of the same type for research. Cell culture plays an important role in life science research, such as cancer research, pharmacology, genetics, physiology and other fields. This technology requires the use of some basic equipment and containers, such as clean benches, biosafety cabinets, cell culture incubators, microscopes, cell culture plates, cell bottles and cell dishes. The selection of appropriate culture media and additives can provide the nutrients and growth factors required for cell proliferation and growth. Maintaining factors such as temperature, humidity and carbon dioxide concentration in the culture environment is also very important for cell growth and expansion.

[0003] In the prior art, cell culture reactors are mainly divided into stirred cell culture reactors, airlift cell culture reactors, disposable cell culture reactors, photobioreactors, and other related new reactors. The most commonly used of these reactors are stirred cell culture reactors and airlift cell culture reactors, among which:

[0004] The stirred reactor is the earliest developed cell culture reactor, which mainly mixes the culture medium and cells by mechanical stirring. This reactor has a good mass transfer effect and can effectively increase the growth rate and yield of cells. However, mechanical stirring can easily cause shear damage to cells, especially for suspended cells and sensitive cells. This damage may affect cell growth and product quality.

[0005] Airlift reactors use gas flow to mix culture medium and cells, reducing shear damage caused by mechanical stirring. Airlift reactors have the advantages of simple operation and low energy consumption, and are suitable for large-scale cell culture. However, due to the introduction of gas, the dissolved oxygen control of airlift reactors is more complicated, requiring precise control of gas flow rate and bubble size.

[0006] In response to these problems in the prior art, we proposed a new type of vibration cell culture reactor. Summary of the invention

[0007] The purpose of this section is to summarize some aspects of embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the specification abstract and the invention title of this application to avoid blurring the purpose of this section, the specification abstract and the invention title, and such simplifications or omissions cannot be used to limit the scope of the present invention.

[0008] In view of the above problems and / or the problems existing in the prior art, the present invention is proposed.

[0009] Therefore, the technical problem to be solved by the present invention is to reduce shear force damage during cell culture, improve mixing effect, simplify operation and maintenance, and optimize energy consumption by adopting a vibrating structure.

[0010] In order to solve the above technical problems, the present invention provides the following technical solutions: a cell culture reactor, comprising a box, a reaction mechanism and a vibration mechanism, wherein a first cavity is formed at the top of the box, and a second cavity is formed at the bottom of the box;

[0011] The reaction mechanism comprises a fixing seat fixedly mounted on the bottom wall of the first cavity, a lifting seat is movably connected inside the fixing seat, a reaction tank is threadedly connected to the top of the lifting seat, the upper end of the reaction tank is open, a cover plate is arranged on the open end, an air inlet pipe is arranged on the left side of the cover plate, and an exhaust pipe is arranged on the right side of the cover plate, and the output end of the air inlet pipe extends to the interior of the reaction tank, and the input end of the exhaust pipe also extends to the interior of the reaction tank;

[0012] The vibration mechanism includes a servo motor fixedly installed in the left side wall of the box body, the output end of the servo motor is connected to a rotating rod through a coupling transmission, eccentric blocks are fixedly installed on the left and right ends of the outer surface of the rotating rod, the upper surfaces of the two eccentric blocks are abutted against a lifting plate, a push rod is fixedly installed on the top of the lifting plate, and the top of the push rod passes through the inner top wall of the second cavity and extends into the first cavity to abut against the bottom wall of the lifting seat, the bottom ends of the two eccentric blocks are movably connected with lifting blocks, and an adjustment component is arranged between the two lifting blocks.

[0013] As a preferred embodiment of the cell culture reactor of the present invention, the air inlet pipe and the exhaust pipe both vertically pass through the cover plate and are inserted into the interior of the reaction tank, the vertical portions of the air inlet pipe and the exhaust pipe are both hoses, and the vertical portions of the air inlet pipe and the exhaust pipe are both suspended in the upper portion of the first cavity through a fixing ring.

[0014] As a preferred embodiment of the cell culture reactor described in the present invention, lifting blocks are fixedly installed on the left and right sides of the lifting seat, and buffer springs are fixedly connected to the upper and lower sides of the two lifting blocks, and the top end of the upper buffer spring is fixedly connected to the inner top wall of the fixed seat, and the bottom end of the lower buffer spring is fixedly connected to the inner bottom wall of the fixed seat.

[0015] As a preferred solution of the cell culture reactor described in the present invention, limit rods are symmetrically arranged on the left and right sides of the lifting plate, and the outer surfaces of the two limit rods are both sleeved with limit blocks, and the two limit blocks are fixedly connected to the left and right ends of the lifting plate.

[0016] As a preferred embodiment of the cell culture reactor described in the present invention, wherein: a moving cavity is provided inside the two eccentric blocks, a positioning groove is provided on the opposite surfaces of the two eccentric blocks, and three positioning grooves are provided on each of the eccentric blocks, a telescopic groove is provided inside the two lifting blocks, and openings are provided at the top ends of the two telescopic grooves, a moving plate is movably connected inside the two telescopic grooves and on one side of the opening, telescopic springs are fixedly connected to the opposite back surfaces of the two moving plates, and the other ends of the two telescopic springs are respectively connected to the side walls of the two telescopic grooves, the top ends of the two moving plates respectively penetrate the two openings and extend into the two moving cavities, a positioning pin is fixedly installed on the top end of the two moving plates and on one side close to the positioning groove, and the two positioning pins are respectively inserted into the positioning groove described in each group of upper ends.

[0017] As a preferred solution of the cell culture reactor described in the present invention, wherein: the adjustment component includes a mounting sleeve fixedly installed between the two lifting blocks, an extrusion cavity is opened at the center of the mounting sleeve, push plates are symmetrically arranged on the left and right sides of the extrusion cavity, and an extrusion cone is arranged above the two push plates. Push rods are fixedly installed on the opposite back surfaces of the two push plates, and the other ends of the two push rods pass through the inner wall of the mounting sleeve and extend to the inside of the two lifting blocks, and are fixedly connected to the side walls of the two movable plates.

[0018] As a preferred solution of the cell culture reactor of the present invention, the left and right sides of the bottom end of the extrusion cone are symmetrically provided with inclined surfaces, and a tip is provided between the two inclined surfaces, and the tip is provided between the two pushing plates.

[0019] As a preferred embodiment of the cell culture reactor described in the present invention, the top end of the extrusion cone is fixedly connected to a pressing rod, and the top end of the pressing rod passes through the inner top wall of the mounting sleeve and extends to the top thereof to be fixedly connected to a button, the bottom end of the button is fixedly connected to a compression spring, the compression spring is sleeved on the outer surface of the pressing rod, and the bottom end of the compression spring is fixedly connected to the inner top wall of the mounting sleeve.

[0020] As a preferred solution of the cell culture reactor of the present invention, a protective door is provided at the front end of the second cavity, and the protective door is hinged on the front surface of the box body.

[0021] Beneficial effects of the present invention:

[0022] 1. In the present invention, the vibration mechanism is provided to drive the lifting seat to move vertically up and down reciprocatingly, and the reaction tank is installed on the lifting seat, so it can drive the reaction tank containing the cell solution to vibrate up and down reciprocatingly. This design enables the culture fluid and cells to be effectively mixed, promotes the uniform distribution of nutrients and oxygen, and significantly improves the growth rate and yield of cells. At the same time, the shear force generated by the vibration method is small, which reduces the damage to cells compared with traditional mechanical stirring, especially more friendly to suspended cells and sensitive cells.

[0023] 2. In the present invention, the position between the lifting block and the eccentric block can be adjusted by adjusting the assembly, thereby changing the height and frequency of vibration to meet the needs of different cell cultures. This convenient adjustment not only improves the applicability and flexibility of the reactor, but also simplifies the operation and maintenance process, optimizes energy consumption, and makes the reactor more suitable for large-scale cell culture applications. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative labor. Among them:

[0025] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention;

[0026] Figure 2 It is a front cross-sectional view of the present invention;

[0027] Figure 3 It is a schematic diagram of the structure enlargement of the reaction mechanism of the present invention;

[0028] Figure 4 A three-dimensional diagram of the eccentric block and the adjusting assembly of the present invention;

[0029] Figure 5 It is a three-dimensional cross-sectional view of the eccentric block of the present invention;

[0030] Figure 6 A three-dimensional diagram of the connection between the adjustment assembly and the lifting block of the present invention;

[0031] Figure 7 It is a front cross-sectional view of the adjustment component of the present invention. DETAILED DESCRIPTION

[0032] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with the accompanying drawings.

[0033] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0034] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The term "in one embodiment" that appears in different places in this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive with other embodiments.

[0035] Example 1: Reference Figure 1~Figure 4 , which is the first embodiment of the present invention, provides a cell culture reactor, including a box body 100, a first cavity 101 is formed at the top of the box body 100, a second cavity 102 is formed at the bottom of the box body 100, and a reaction mechanism 200 installed in the first cavity 101 and a vibration mechanism 300 installed in the second cavity 102. The reaction mechanism 200 is used to provide a cell culture container and a necessary gas exchange path to ensure the stability and sterility of the culture environment. The vibration mechanism 300 is used to drive the servo motor 301 to move the eccentric The core block 303 rotates, thereby driving the lifting seat 202 and the reaction tank 203 to vibrate up and down, achieving the effect of mixing the culture medium and cells. The box body 100 provides a structural frame to integrate the reaction mechanism 200 and the vibration mechanism 300 together, and separate the two by the first cavity 101 and the second cavity 102, so that the functions of each part are clear and the structure is compact. Through this design, the vibration mechanism 300 can effectively drive the reaction tank 203 to vibrate up and down, ensuring the uniform mixing of the culture medium and cells during the cell culture process, thereby improving the growth efficiency and quality of the cells.

[0036] Specifically, the reaction mechanism 200 includes a fixed seat 201 fixedly mounted on the inner bottom wall of the first cavity 101. The function of the fixed seat 201 is to provide stable support for the reaction mechanism. The fixed seat 201 is internally movably connected with a lifting seat 202. The lifting seat 202 is used to adjust the height and position of the reaction tank 203. The top of the lifting seat 202 is threadedly connected with the reaction tank 203. The reaction tank 203 is used to contain cell culture fluid and cells and provide a culture environment. The upper end of the reaction tank 203 is open, and a cover plate 204 is provided on the open end. The cover plate 204 serves to close the reaction tank. , to prevent contamination and maintain a sterile environment, an air inlet pipe 205 is arranged on the left side inside the cover plate 204, and the function of the air inlet pipe 205 is to introduce gas into the interior of the reaction tank to provide oxygen and other gases required for cell growth. An exhaust pipe 206 is arranged on the right side of the cover plate 204, and the exhaust pipe 206 is used to discharge the waste gas in the reaction tank and adjust the internal pressure. The output end of the air inlet pipe 205 extends to the interior of the reaction tank 203 to ensure that the gas can effectively enter the culture solution, and the input end of the exhaust pipe 206 also extends to the interior of the reaction tank 203 to ensure that the waste gas can be discharged smoothly.

[0037] Furthermore, the vibration mechanism 300 includes a servo motor 301 fixedly installed in the left side wall of the box body 100. The function of the servo motor 301 is to provide a power source and drive other parts to move through rotation output. The output end of the servo motor 301 is connected to a rotating rod 302 through a coupling transmission. The function of the coupling is to connect the motor and the rotating rod 302 to transmit the power of the motor. Eccentric blocks 303 are fixedly installed on the left and right ends of the outer surface of the rotating rod 302. The eccentric blocks 303 are used to generate uneven rotational motion, which is converted into up and down vibration. The upper surfaces of the two eccentric blocks 303 are abutted against lifting plates 304. The lifting plates 304 realize up and down motion under the drive of the eccentric blocks 303. The top of the lifting plates 304 A top rod 305 is fixedly installed, and the function of the top rod 305 is to transmit the movement of the lifting plate 304 to the lifting seat 202. The top end of the top rod 305 passes through the inner top wall of the second cavity 102, and extends to the first cavity 101 to abut against the bottom wall of the lifting seat 202, so that the lifting seat 202 moves up and down with the lifting plate 304. The bottom ends of the two eccentric blocks 303 are movably connected with a lifting block 306. The function of the lifting block 306 is to support and cooperate with the movement of the eccentric block 303 and transmit force to the lifting plate 304. An adjustment component 307 is arranged between the two lifting blocks 306. The adjustment component 307 is used to adjust the position between the lifting block 306 and the eccentric block 303 to change the amplitude and frequency of vibration.

[0038] In summary, the vibration mechanism 300 is powered by the servo motor 301, which is connected to the rotating rod 302 via a coupling transmission, driving the eccentric blocks 303 fixed at both ends of the rotating rod to produce uneven rotational motion. The eccentric blocks 303 push the lifting plate 304 to move up and down, and the lifting plate 304 transmits the motion to the lifting seat 202 through the push rod 305, driving the reaction tank 203 to vibrate up and down, and the lifting block 306 supports and cooperates with the movement of the eccentric block 303. The adjustment component 307 is used to adjust the position between the lifting block 306 and the eccentric block 303 to control the amplitude and frequency of the vibration, thereby achieving precise vibration control of the reaction tank 203 and ensuring uniform mixing of the culture medium and cells.

[0039] It should be noted that both the air inlet pipe 205 and the exhaust pipe 206 are vertically passed through the cover plate 204 and inserted into the reaction tank 203, and the cover plate 204 is slidably connected with the air inlet pipe 205 and the exhaust pipe 206, so that when the reaction tank 203 drives the cover plate 204 to vibrate up and down, the air inlet pipe 205 and the exhaust pipe 206 will not block the cover plate 204 from moving up and down, ensuring that the reaction tank 203 can vibrate stably. Secondly, the vertical parts of the air inlet pipe 205 and the exhaust pipe 206 are both hoses, which makes it easier for the staff to open the cover plate 204. , the air inlet pipe 205 and the exhaust pipe 206 can be easily bent and taken out from the inside of the reaction tank 203. During the taking out process, it is convenient for the staff to rotate and remove the reaction tank 203 from the lifting seat 202. Finally, the vertical parts of the air inlet pipe 205 and the exhaust pipe 206 are suspended in the upper part of the first cavity 101 through the fixing ring 207. The function of the fixing ring 207 is to suspend and support the air inlet pipe 205 and the exhaust pipe 206 so that they are not hindered when the cover plate 204 moves up and down, and to prevent the hose from being displaced or damaged due to gravity or vibration.

[0040] Preferably, lifting blocks 208 are installed on the left and right sides of the lifting seat 202 to provide support and guiding functions so that the lifting seat 202 can move up and down smoothly. The buffer spring 209 is connected to the upper and lower sides of the lifting block 208, one end of which is fixed to the inner top wall of the fixed seat 201, and the other end is fixed to the inner bottom wall of the fixed seat 201. The vibration and impact of the lifting seat 202 are absorbed and buffered through elastic action. This design ensures that the lifting seat 202 can move up and down smoothly and without impact when driven by the vibration mechanism 300, thereby improving the stability of the reaction tank 203 and the safety of the cell culture process.

[0041] Preferably, limit rods 308 are symmetrically arranged on the left and right sides of the lifting plate 304. The function of the limit rods 308 is to limit the horizontal movement of the lifting plate 304 to ensure that it can only move in the vertical direction. The outer surface of each limit rod 308 is sleeved with a limit block 309. The function of the limit block 309 is to provide a stable sliding interface so that the lifting plate 304 is constrained by the limit rod 308 when moving up and down to prevent it from horizontal displacement. The two limit blocks 309 are respectively fixedly connected to the left and right ends of the lifting plate 304. Through this arrangement, the limit rods 308 and the limit blocks 309 work together to ensure that the lifting plate 304 maintains the correct motion trajectory during the vibration process.

[0042] In summary, the working principle of the device is as follows: First, open the cover plate 204 and add the cell culture fluid and cell suspension into the reaction tank 203. Ensure that the interior of the reaction tank 203 is sterile and the liquid level is appropriate. Then, cover the cover plate 204 again, and ensure that the air inlet pipe 205 and the exhaust pipe 206 are correctly inserted into the interior of the reaction tank 203 through a sliding connection, and keep the sealed state. Secondly, start the servo motor 301, and the servo motor 301 drives the rotating rod 302 to rotate through the coupling. The eccentric block 303 on the rotating rod 302 produces uneven movement during the rotation process, pushing the lifting plate 304 to vibrate up and down. The lifting plate 304 transmits the vibration to the lifting seat 202 through the top rod 305, driving the reaction tank 203 to vibrate up and down. During the vibration process, the buffer spring 209 absorbs and buffers the vibration and impact of the lifting seat 202 to ensure the stability of the lifting seat 202 and the reaction tank 203. The limiting rod 308 and the limiting block 309 work together to limit the horizontal movement of the lifting plate 304, ensuring its stable movement in the vertical direction. During the vibration process, the air inlet pipe 205 continuously supplies oxygen to the reaction tank 203, and the exhaust pipe 206 discharges exhaust gas, ensuring smooth gas exchange in the reaction tank 203 and maintaining a suitable culture environment. By adjusting the component 307, the position between the lifting block 306 and the eccentric block 303 can be adjusted to control the amplitude and frequency of the vibration, thereby achieving precise vibration control of the reaction tank 203 and ensuring uniform mixing of the culture medium and cells. Finally, after completing the cell culture, stop the servo motor 301, open the cover 204 again, remove the air inlet pipe 205 and the exhaust pipe 206 from the reaction tank 203 by bending the hose, and then rotate the reaction tank 203 off the lifting seat 202 to collect cells and perform subsequent processing.

[0043] Example 2: Reference Figure 4~Figure 7 , which is the second embodiment of the present invention, and this embodiment is based on the previous embodiment.

[0044] Specifically, the two eccentric blocks 303 are provided with a moving cavity 303a inside, and the function of the moving cavity 303a is to provide space for the moving plate 310 to move inside the eccentric block 303. The opposite surfaces of the two eccentric blocks 303 are provided with a positioning groove 303b, and the function of the positioning groove 303b is to fix the position of the positioning pin 312 to ensure the movement trajectory of the moving plate 310. There are three positioning grooves 303b on each eccentric block 303 to facilitate flexible adjustment and fixing of the position. The interiors of the two lifting blocks 306 are provided with telescopic grooves. 306a, the telescopic slot 306a is used to accommodate the moving plate 310 and provide a movement path for the moving plate 310. The top of the telescopic slot 306a is provided with an opening 306b, and the opening 306b allows the moving plate 310 to extend into the moving cavity 303a. The inside of the two telescopic slots 306a and one side of the opening 306b are movably connected with the moving plate 310. The moving plate 310 moves in the telescopic slot 306a and extends into the moving cavity 303a. The opposite backs of the two moving plates 310 are fixedly connected with telescopic elastic The function of the telescopic spring 311 is to provide elastic restoring force so that the moving plate 310 can return to the initial position after movement. The other ends of the two telescopic springs 311 are respectively connected to the side walls of the two telescopic slots 306a to provide fixed support. The top ends of the two moving plates 310 respectively pass through the two openings 306b and extend into the two moving cavities 303a to ensure that the moving plate 310 can move freely in the eccentric block 303. The top ends of the two moving plates 310 and one side close to the positioning slot 303b are fixedly installed with positioning Pin 312, the function of the positioning pin 312 is to fix the movable plate 310 at a predetermined position in the positioning groove 303b, so that the lifting block 306 sleeved on the bottom end of the movable plate 310 can be relatively fixed with the eccentric block 303, and then when the eccentric block 303 rotates, the lifting block 306 can move up and down accordingly, thereby realizing vibration control of the lifting plate 304 and the reaction tank 203. The two positioning pins 312 are respectively inserted into the positioning groove 303b at the upper end of each group to ensure the accurate positioning and fixation of the movable plate 310 in the eccentric block 303.

[0045] Furthermore, the adjustment component 307 includes a mounting sleeve 307a fixedly mounted between the two lifting blocks 306. The mounting sleeve 307a is used to fix the adjustment component in an appropriate position and provide structural support. An extrusion cavity 307b is provided at the center of the mounting sleeve 307a. The extrusion cavity 307b is used to accommodate and guide the movement of the push plate 307c. The push plates 307c are symmetrically arranged on the left and right sides of the extrusion cavity 307b. The push plates 307c are used to move left and right in the extrusion cavity, thereby pushing other components. There is an extrusion cone 307d, and the function of the extrusion cone 307d is to push the push plate 307c to both sides through its conical structure to generate an extrusion force. Push rods 307e are fixedly installed on the opposite back surfaces of the two push plates 307c. The push rods 307e are used to transmit the movement of the push plates 307c. The other ends of the two push rods 307e pass through the inner wall of the mounting sleeve 307a and extend to the inside of the two lifting blocks 306, and are fixedly connected to the side walls of the two moving plates 310. Through the transmission of the push rods 307e, the moving plates 310 can move with the movement of the push plates 307c.

[0046] Furthermore, inclined surfaces are symmetrically arranged on the left and right sides of the bottom of the extrusion cone 307d, and a tip is arranged between the two inclined surfaces, and the tip is arranged between the two push plates 307c. The design of the inclined surface and the tip enables the extrusion cone 307d to effectively push the push plates 307c to both sides when it moves downward, thereby promoting the movement of the push rod 307e and the moving plate 310.

[0047] It should be noted that when the extrusion cone 307d moves vertically downward, the push plate 307c is pushed to both sides by its inclined surface guiding action, and the two push plates 307c can drive the two moving plates 310 to move to the outside of the two lifting blocks 306 through the two push rods 307e, and the two lifting blocks 306 can drive the two positioning pins 312 to move out of the positioning groove 303b (such as Figure 5 As shown), the limiting fixation between the movable plate 310 and the eccentric block 303 is released, and the relative fixation between the lifting block 306 and the eccentric block 303 is also released, so that the position of the lifting block 306 is adjusted to be farther away from the axis of the eccentric block 303, which can increase the height of the long axis end of the eccentric block 303 and expand the vibration amplitude.

[0048] Furthermore, the top end of the extrusion cone 307d is fixedly connected to a pressing rod 307f, and the function of the pressing rod 307f is to transmit the movement of the extrusion cone 307d to the outside and adjust it through external control. The top end of the pressing rod 307f passes through the inner top wall of the mounting sleeve 307a, and extends to the top thereof where a button 307g is fixedly connected. The function of the button 307g is to provide a convenient operating interface for the staff to press for adjustment. The bottom end of the button 307g is fixedly connected to a compression spring 307h, and the function of the compression spring 307h is to provide an elastic reset force so that the button 307g and the pressing rod 307f can automatically return to their original positions after being pressed. The compression spring 307h is sleeved on the outer surface of the pressing rod 307f, and the bottom end of the compression spring 307h is fixedly connected to the inner top wall of the mounting sleeve 307a, to ensure that the compression spring 307h can provide a stable elastic force when pressed.

[0049] Through this adjustment mechanism, when the staff presses the button 307g, the pressing rod 307f pushes the extrusion cone 307d downward, and the inclined surface of the extrusion cone 307d pushes the push plate 307c away. The push plate 307c moves the lifting block 306 and the moving plate 310 through the push rod 307e, so that the lifting block 306 is released from the eccentric block 303. After releasing the button 307g, the compression spring 307h restores the pressing rod 307f and the extrusion cone 307d to the initial position, and the push plate 307c and the lifting block 306 are also reset, completing the adjustment process.

[0050] Preferably, a protective door 103 is provided at the front end of the second cavity 102. The function of the protective door 103 is to protect the internal vibration mechanism 300 and prevent dust, foreign matter and other external factors from entering, thereby ensuring the normal operation of the equipment. The protective door 103 is hinged to the front surface of the box body 100 through a hinge, so that it can be easily opened and closed, which is convenient for operation and maintenance.

[0051] In summary, when the vibration amplitude of the reaction tank 203 needs to be adjusted, first, the protective door 103 is opened to expose the internal adjustment component 307, and the button 307g is pressed. The pressing rod 307f pushes the extrusion cone 307d downward, and the inclined surface of the extrusion cone 307d pushes the push plate 307c to both sides. The push plate 307c moves the lifting block 306 and the moving plate 310 through the push rod 307e, so that the lifting block 306 and the eccentric block 303 are released from the limit fixation.

[0052] After the lifting block 306 and the eccentric block 303 are released from the limit fixation, the installation sleeve 307a is held and moved downward, thereby driving the lifting blocks 306 on both sides to move downward, and continue to move until the two positioning pins 312 move to the next positioning groove 303b, then release the button 307g, and the compression spring 307h restores the pressing rod 307f and the extrusion cone 307d to the initial position, and without the obstruction of the extrusion cone 307d, the moving plate 310, the push rod 307e and the push plate 307c are extended. Under the elastic force of the compression spring 311, it will also reset, and the two movable plates 310 will be able to drive the two positioning pins 312 to insert into the new positioning groove 303b, fix the position of the movable plate 310, and then the lifting block 306 and the eccentric block 303 will be re-fixed in the new position. After the adjustment is completed, the protective door 103 is closed to ensure the safety of the internal vibration mechanism 300 and the normal operation of the equipment. In this way, the position of the lifting block 306 can be adjusted, and the height of the long axis end of the eccentric block 303 can be increased or decreased, thereby adjusting the vibration amplitude.

[0053] Importantly, it should be noted that the construction and arrangement of the present application shown in a number of different exemplary embodiments are only exemplary. Although only a few embodiments are described in detail in this disclosure, it should be readily understood by those who refer to this disclosure that many modifications are possible (e.g., the size, scale, structure, shape and proportion of various elements, and parameter values ​​(e.g., temperature, pressure, etc.), mounting arrangements, use of materials, color, changes in orientation, etc.) without substantially departing from the novel teachings and advantages of the subject matter described in the application. For example, an element shown as integrally formed may be composed of multiple parts or elements, the position of the element may be inverted or otherwise changed, and the nature or number or position of the discrete element may be altered or changed. Therefore, all such modifications are intended to be included within the scope of the present invention. The order or sequence of any process or method steps may be changed or reordered according to an alternative embodiment. In the claims, any "device plus function" clause is intended to cover the structure described herein that performs the function, and is not only structurally equivalent but also equivalent structure. Other substitutions, modifications, changes and omissions may be made in the design, operating conditions and arrangement of the exemplary embodiments without departing from the scope of the present invention. Therefore, the invention is not limited to a specific embodiment, but extends to numerous modifications still falling within the scope of the appended claims.

[0054] Additionally, in order to provide a concise description of exemplary embodiments, all features of an actual embodiment may not be described (ie, those features that are not relevant to the best mode presently contemplated for carrying out the invention or those features that are not relevant to implementing the invention).

[0055] It will be appreciated that in the development of any actual implementation, as in any engineering or design project, numerous implementation-specific decisions may be made. Such a development effort may be complex and time-consuming, but will be a routine task of design, fabrication, and production for those of ordinary skill having the benefit of this disclosure without undue experimentation.

[0056] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A cell culture reactor, characterized in that: include, A box body (100), wherein a first cavity (101) is provided at the top end of the box body (100), and a second cavity (102) is provided at the bottom end of the box body (100); The reaction mechanism (200) comprises a fixing seat (201) fixedly mounted on the inner bottom wall of the first cavity (101), the fixing seat (201) being movably connected to a lifting seat (202) inside, the top end of the lifting seat (202) being threadedly connected to a reaction tank (203), the upper end of the reaction tank (203) being open, a cover plate (204) being arranged on the open end, an air intake pipe (205) being arranged on the left side inside the cover plate (204), and an exhaust pipe (206) being arranged on the right side of the cover plate (204), the output end of the air intake pipe (205) extending into the interior of the reaction tank (203), and the input end of the exhaust pipe (206) also extending into the interior of the reaction tank (203); and, The vibration mechanism (300) comprises a servo motor (301) fixedly mounted in the left side wall of the box body (100); the output end of the servo motor (301) is connected to a rotating rod (302) through a coupling transmission; eccentric blocks (303) are fixedly mounted on the left and right ends of the outer surface of the rotating rod (302); the upper surfaces of the two eccentric blocks (303) are in contact with a lifting plate (304); a top rod (305) is fixedly mounted on the top of the lifting plate (304); the top end of the top rod (305) passes through the inner top wall of the second cavity (102) and extends into the first cavity (101) to abut against the bottom wall of the lifting seat (202); the bottom ends of the two eccentric blocks (303) are movably connected to a lifting block (306), and an adjustment component (307) is provided between the two lifting blocks (306); The two eccentric blocks (303) are each provided with a moving cavity (303a) inside, the opposite surfaces of the two eccentric blocks (303) are each provided with a positioning groove (303b), and each of the eccentric blocks (303) has three positioning grooves (303b) therein, the two lifting blocks (306) are each provided with a telescopic groove (306a), and the top ends of the two telescopic grooves (306a) are each provided with an opening (306b), a moving plate (310) is movably connected inside the two telescopic grooves (306a) and on one side of the opening (306b), and the two lifting blocks (306) are each provided with a telescopic groove (306a). The opposite back surfaces of the moving plates (310) are fixedly connected with telescopic springs (311), and the other ends of the two telescopic springs (311) are respectively connected with the side walls of the two telescopic slots (306a), the top ends of the two moving plates (310) respectively penetrate the two openings (306b) and extend into the two moving cavities (303a), and the top ends of the two moving plates (310) and one side close to the positioning slots (303b) are fixedly installed with positioning pins (312), and the two positioning pins (312) are respectively inserted into the positioning slots (303b) at the upper end of each group; The adjustment component (307) comprises a mounting sleeve (307a) fixedly mounted between the two lifting blocks (306); an extrusion cavity (307b) is provided at the center of the mounting sleeve (307a); push plates (307c) are symmetrically arranged on the left and right sides of the extrusion cavity (307b); an extrusion cone (307d) is arranged above the two push plates (307c); push rods (307e) are fixedly mounted on opposite back surfaces of the two push plates (307c); the other ends of the two push rods (307e) penetrate the inner wall of the mounting sleeve (307a) and extend into the interior of the two lifting blocks (306), and are fixedly connected to the side walls of the two movable plates (310); Inclined surfaces are symmetrically arranged on the left and right sides of the bottom end of the extrusion cone (307d), and a tip is arranged between the two inclined surfaces, and the tip is arranged between the two push plates (307c); The top end of the extrusion cone (307d) is fixedly connected to a pressing rod (307f), and the top end of the pressing rod (307f) passes through the inner top wall of the mounting sleeve (307a) and extends to the top thereof to be fixedly connected to a button (307g), and the bottom end of the button (307g) is fixedly connected to a compression spring (307h), the compression spring (307h) is sleeved on the outer surface of the pressing rod (307f), and the bottom end of the compression spring (307h) is fixedly connected to the inner top wall of the mounting sleeve (307a).

2. The cell culture reactor according to claim 1, characterized in that: The air intake pipe (205) and the exhaust pipe (206) are both vertically passed through the cover plate (204) and inserted into the interior of the reaction tank (203); the vertical parts of the air intake pipe (205) and the exhaust pipe (206) are both hoses, and the vertical parts of the air intake pipe (205) and the exhaust pipe (206) are both suspended in the upper part of the first cavity (101) via a fixing ring (207).

3. The cell culture reactor according to claim 2, characterized in that: Lifting blocks (208) are fixedly mounted on both left and right sides of the lifting seat (202), and buffer springs (209) are fixedly connected to both upper and lower sides of the two lifting blocks (208), and the top end of the buffer spring (209) at the upper end is fixedly connected to the inner top wall of the fixed seat (201), and the bottom end of the buffer spring (209) at the lower end is fixedly connected to the inner bottom wall of the fixed seat (201).

4. The cell culture reactor according to claim 3, characterized in that: Limiting rods (308) are symmetrically arranged on the left and right sides of the lifting plate (304), and the outer surfaces of the two limiting rods (308) are sleeved with limiting blocks (309), and the two limiting blocks (309) are fixedly connected to the left and right ends of the lifting plate (304).

5. The cell culture reactor according to claim 4, characterized in that: A protective door (103) is provided at the front end of the second cavity (102), and the protective door (103) is hinged on the front surface of the box body (100).

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