Essence containing osmanthus extract with soothing, firming and anti-wrinkle and lightening effects and preparation method thereof

Abstract

The present invention relates to the technical field of daily chemical products, and specifically provides an essence containing osmanthus extract with soothing, firming and anti-wrinkle and light lines and a preparation method. The essence is measured by mass, including: 10 to 30 parts of osmanthus extract, 2 to 10 parts of frankincense seed extract, 2 to 10 parts of betaine, 2 to 10 parts of pentapeptide, 1 to 5 parts of hydrogenated soybean lecithin, 1 to 5 parts of xylitol, and 60 to 80 parts of water. Compared with the prior art, the essence containing osmanthus extract with soothing, firming and anti-wrinkle and light lines of the present invention not only has a good anti-inflammatory effect, but also can significantly reduce the content of MMP-1, thereby reducing the degradation of collagen, is conducive to increasing skin elasticity, improving skin firmness, repairing damaged skin, promoting healing, and has anti-aging and repairing effects.

Description

Technical Field

[0001] The invention relates to the technical field of daily chemical products, and more particularly to an essence containing osmanthus extract which has the functions of soothing, firming, anti-wrinkle and lightening lines, and a preparation method thereof. Background Art

[0002] As the surface of the human body in contact with the outside world, the skin plays a very important role in protecting and maintaining the normal circulation and metabolism of the human body. The skin can be roughly divided into three layers, from the inside to the outside, namely the subcutaneous tissue, the dermis, and the epidermis. Fibroblasts (the main cells of the dermis) are specialized in the synthesis of these elastic fibers and collagen. Between the ages of 20 and 80, the number of fibroblasts is reduced by half. They ensure the balance between the synthesis, maturation and degradation of elastic fibers and collagen. Over time, this balance will move towards the degradation of these fibers, resulting in the loss of elasticity and tension of the dermis and relaxation, which no longer counteracts the effect of muscle contraction underneath, causing the skin to become thinner and form wrinkles.

[0003] Collagen is the main component of the dermis of human skin, of which type I collagen accounts for 80%-85% of the collagen content of the skin. Type I collagen can increase the tensile strength of the skin and is one of the key components for maintaining skin firmness. With age, the balance between the production and degradation of important biological molecules such as collagen in the skin is broken, gradually leaning towards the degradation process, resulting in progressive thinning and tissue damage of the dermis, and causing dermal relaxation and subsequent wrinkle formation. Under the influence of ultraviolet radiation, collagen synthesis is inhibited, matrix metalloproteinases (MMPs) are highly expressed, and collagen in the dermis is severely damaged, resulting in the destruction of dermal integrity, reduced skin strength and elasticity, and further accelerated skin aging. Summary of the invention

[0004] In view of this, the purpose of the present invention is to provide an essence containing osmanthus extract with soothing, firming and anti-wrinkle and line-lightening effects and a preparation method thereof. The essence containing osmanthus extract with soothing, firming and anti-wrinkle and line-lightening effects of the present invention can not only promote the secretion of collagen by cells, but also can significantly reduce the content of MMP-1, thereby reducing the degradation of collagen, which is beneficial to increase skin elasticity, improve skin firmness, repair damaged skin, promote healing, and has anti-aging and repair effects.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] The essence containing osmanthus extract with soothing, firming and anti-wrinkle and line-lightening effects comprises, by mass, 10 to 30 parts of osmanthus extract, 2 to 10 parts of frankincense seed extract, 2 to 10 parts of betaine, 2 to 10 parts of pentapeptide, 1 to 5 parts of hydrogenated soybean lecithin, 1 to 5 parts of xylitol, and 60 to 80 parts of water; the solid content of the osmanthus extract is 3 to 5 mg / mL.

[0007] Schinus terebinthifolius seeds are the seeds of Schinus terebinthifolius. Schinus terebinthifolius, also known as Brazilian pepper or Brazilian pepper tree, is a common plant native to Brazil and other parts of South America. It is an evergreen shrub or small tree with bright red fruits and dark green leaves. The seeds and leaves of Schinus terebinthifolius contain some beneficial ingredients, such as volatile oils, tannins and phenolic compounds, which give Schinus terebinthifolius some medicinal and flavoring uses. Schinus terebinthifolius seeds can be used as a medicinal plant with antioxidant and other effects.

[0008] Betaine is a natural compound that is often used in cosmetics as a moisturizer and anti-irritant. It can help regulate the osmotic pressure of cosmetics, thereby playing a moisturizing role, keeping the skin hydrated, and improving the softness and smoothness of the skin. In addition, betaine also has soothing and anti-irritant properties, which helps to reduce skin discomfort and has a certain protective effect on sensitive skin.

[0009] Pentapeptide, CAS number is 214047-00-4. Pentapeptide can stimulate the body to produce specific immune response, inhibit the release of neurotransmitters, reduce facial muscle contraction, and play a good antioxidant and anti-wrinkle effect; Pentapeptide can also promote collagen synthesis and regeneration, and has a good improvement effect on the skin.

[0010] Preferably, the osmanthus is golden osmanthus (Laurus nobilis L.).

[0011] Preferably, the osmanthus fragrans is flowers and / or leaves of osmanthus fragrans.

[0012] Preferably, the preparation method of the osmanthus extract comprises:

[0013] The osmanthus flowers are crushed, mixed with water, and distilled under reduced pressure to obtain a distillate, and the distillate is concentrated to a solid content of 3 to 5 mg / mL to obtain an osmanthus flower extract;

[0014] The material-liquid ratio of the osmanthus to water is 1:(10-20) g / mL; the temperature of the reduced pressure distillation is 80-90° C., the time is 2-3 hours, and the pressure is -0.09--0.1 MPa.

[0015] Preferably, the preparation method of the osmanthus extract comprises the following steps:

[0016] (1) Take fresh osmanthus fragrans (Laurus nobilis L.) flowers, clean the dust on the surface of the petals, crush them, place them in a steam generator, and heat them under 90°C steam for 10 minutes.

[0017] (2) placing the osmanthus powder obtained in step (1) in a distillation flask of a rotary evaporator, adding deionized water to the distillation flask, the solid-liquid ratio of osmanthus powder to deionized water being 1:10 g / mL, and then performing reduced pressure distillation at 80° C. and −0.09 MPa for 3 h, controlling the speed of the rotary evaporator to be 70 r / min; repeating the above operation, performing secondary distillation, mixing the distillates of the two distillations to obtain a mixed solution, sterilizing, concentrating, and controlling the solid content of the mixed solution to be 5 mg / mL, to obtain an osmanthus extract.

[0018] Preferably, the preparation method of the Boswellia serrata seed extract comprises:

[0019] The frankincense seeds are mixed with ethanol, extracted at 50-60°C for 1-2 hours, and concentrated under reduced pressure; the obtained product is freeze-dried to obtain the frankincense seed extract.

[0020] In an embodiment of the present invention, the preparation method of the frankincense seed extract comprises: taking frankincense seeds, crushing, adding 45% v / v ethanol solution, the solid-liquid ratio of frankincense seed powder to ethanol solution is 1: (8-10) g / ml, extracting twice at 50-60° C., each time for 30 minutes, concentrating under reduced pressure, freezing the product at -18° C. for 24 hours, and sublimating and analytical drying conditions are -10° C., 50 Pa, 30° C., 20 Pa, respectively, to obtain the frankincense seed extract.

[0021] Preferably, the essence containing osmanthus extract with soothing, firming and anti-wrinkle and line-lightening effects further comprises a preservative and / or a stabilizer; the preservative comprises phenoxyethanol; and the stabilizer comprises disodium EDTA.

[0022] Preferably, the essence containing osmanthus extract with soothing, firming and anti-wrinkle and lightening effects comprises, by parts by mass:

[0023] 10 parts of osmanthus extract, 6 parts of frankincense seed extract, 4 parts of betaine, 5 parts of pentapeptide, 2 parts of hydrogenated soybean lecithin, 2 parts of xylitol, 0.5 parts of disodium EDTA, 1 part of phenoxyethanol, 69.5 parts of water;

[0024] Alternatively, the essence containing osmanthus extract having the effects of soothing, firming and anti-wrinkle and lightening lines comprises, by parts by mass:

[0025] 20 parts of osmanthus extract, 5 parts of frankincense seed extract, 3 parts of betaine, 4 parts of pentapeptide, 2 parts of hydrogenated soybean lecithin, 2 parts of xylitol, 0.5 parts of disodium EDTA, 1 part of phenoxyethanol, 62.5 parts of water;

[0026] Alternatively, the essence containing osmanthus extract having the effects of soothing, firming and anti-wrinkle and lightening lines comprises, by parts by mass:

[0027] 15 parts of osmanthus extract, 4 parts of frankincense seed extract, 3 parts of betaine, 6 parts of pentapeptide, 2 parts of hydrogenated soybean lecithin, 2 parts of xylitol, 0.5 parts of disodium EDTA, 1 part of phenoxyethanol, and 66.5 parts of water.

[0028] The present invention also provides a method for preparing the essence containing osmanthus extract with soothing, firming and anti-wrinkle and lightening effects, comprising:

[0029] (1) mixing osmanthus extract, frankincense seed extract, betaine, pentapeptide and water, and heating to 50° C. to obtain an aqueous phase;

[0030] Mix hydrogenated soybean lecithin, disodium EDTA, xylitol and phenoxyethanol, and heat to 50° C. to obtain an oil phase;

[0031] (2) mixing the oil phase and the water phase, and shearing for 5 to 15 minutes at 10,000 to 12,000 rpm to obtain liposome colostrum;

[0032] (3) The liposome colostrum is homogenized under high pressure at 500 to 600 bar to obtain an essence containing osmanthus extract having soothing, firming and anti-wrinkle and line-reducing effects.

[0033] Preferably, the method for preparing the essence containing osmanthus extract with soothing, firming and anti-wrinkle and lightening effects comprises:

[0034] (1) Weigh osmanthus extract, frankincense seed extract, betaine and pentapeptide respectively, add them into water, heat to 50° C., and stir to mix the system thoroughly;

[0035] (2) Weigh hydrogenated soybean lecithin, disodium EDTA, xylitol and phenoxyethanol respectively, mix them, heat the system to 50° C., and stir to mix the system;

[0036] (3) adding the oil phase to the water phase and shearing at 10,000 rpm for 9 minutes to obtain liposome colostrum;

[0037] (4) The liposome colostrum was homogenized under high pressure for 3 times at 500 bar to obtain an essence containing osmanthus extract having soothing, firming and anti-wrinkle properties.

[0038] The present invention also provides the application of the essence containing osmanthus extract with soothing, firming and anti-wrinkle and anti-line functions in anti-inflammation.

[0039] Preferably, the inflammatory factor includes TNF-α.

[0040] Preferably, the concentration of the essence containing osmanthus extract with soothing, firming and anti-wrinkle and lightening effects is 10-20wt%.

[0041] The present invention also provides the application of the essence containing osmanthus extract with soothing, firming and anti-wrinkle and anti-line functions in anti-aging.

[0042] The present invention also provides the use of the essence containing osmanthus extract with soothing, firming and anti-wrinkle and lightening effects in reducing the content of matrix metalloproteinases and / or promoting collagen secretion.

[0043] Preferably, the collagen comprises type I collagen.

[0044] Preferably, the concentration of the essence containing osmanthus extract with soothing, firming and anti-wrinkle and lightening effects is 10-20wt%.

[0045] Compared with the prior art, the essence containing osmanthus extract of the present invention with the functions of soothing, firming and anti-wrinkle and line-lightening contains osmanthus extract, frankincense seed extract, pentapeptide and betaine, a total of 4 effective ingredients, and the effective ingredients are then encapsulated by liposomes, which not only has a good anti-inflammatory effect, but also can significantly reduce the content of MMP-1, thereby reducing the degradation of collagen, which is beneficial to increasing skin elasticity, improving skin firmness, repairing damaged skin, promoting healing, and has anti-aging and repair effects. DETAILED DESCRIPTION

[0046] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0047] In order to further illustrate the present invention, the following examples are used to explain the present invention in detail. The raw materials used in the following examples and comparative examples of the present invention are all commercially available products; the raw material companies listed below are one of the ways to purchase the raw materials.

[0048] Betaine used in the examples and comparative examples of the present invention was purchased from Shanghai Yuanye Biotechnology Co., Ltd. with a product number of S18046; pentapeptide was purchased from Shanghai Yuanye Biotechnology Co., Ltd. with a product number of B28580.

[0049] The preparation method of the osmanthus extract used in the examples and comparative examples of the present invention comprises the following steps:

[0050] (1) Take fresh osmanthus fragrans (Laurus nobilis L.) flowers, clean the dust on the surface of the petals, crush them, place them in a steam generator, and heat them under 90°C steam for 10 minutes.

[0051] (2) placing the osmanthus powder obtained in step (1) in a distillation flask of a rotary evaporator, adding deionized water to the distillation flask, the solid-liquid ratio of osmanthus powder to deionized water being 1:10 g / mL, and then performing reduced pressure distillation at 80° C. and −0.09 MPa for 3 h, controlling the speed of the rotary evaporator to be 70 r / min; repeating the above operation, performing secondary distillation, mixing the distillates of the two distillations to obtain a mixed solution, sterilizing, concentrating, and controlling the solid content of the mixed solution to be 5 mg / mL, to obtain an osmanthus extract.

[0052] The preparation method of the Boswellia serrata seed extract used in the embodiments and comparative examples of the present invention comprises the following steps:

[0053] Take Boswellia serrata seeds, grind them, add 45% v / v ethanol solution, the solid-liquid ratio of Boswellia serrata seed powder to ethanol solution is 1:8 g / ml, extract twice at 60°C, each time for 30 min, concentrate under reduced pressure, freeze the product at -18°C for 24 h, and the conditions of sublimation and analytical drying are -10°C, 50 Pa, 30°C, 20 Pa, respectively, to obtain Boswellia serrata seed extract.

[0054] Examples 1-3

[0055] The raw materials and contents of the essence containing osmanthus extract with soothing, firming and anti-wrinkle and line-lightening effects of Examples 1-3 of the present invention are shown in Table 1.

[0056] Table 1 Raw material content (%)

[0057] name Example 1 Example 2 Example 3 water 69.5 62.5 66.5 Osmanthus Extract 10 20 15 Boswellia serrata Seed Extract 6 5 4 Betaine 4 3 3 Five peptides 5 4 6 Hydrogenated soybean lecithin 2 2 2 Xylitol 2 2 2 Phenoxyethanol 1 1 1 Disodium EDTA 0.5 0.5 0.5

[0058] The preparation method of the essence containing osmanthus extract with soothing, firming and anti-wrinkle and lightening effects of Examples 1-3 comprises the following steps:

[0059] (1) According to the ratio in Table 1, weigh osmanthus extract, frankincense seed extract, betaine and pentapeptide respectively, add them into water, heat to 50° C., and stir to mix the system thoroughly;

[0060] (2) Weigh hydrogenated soybean lecithin, disodium EDTA, xylitol and phenoxyethanol respectively, mix them, heat the system to 50° C., and stir to mix the system;

[0061] (3) adding the oil phase to the water phase and shearing at 10,000 rpm for 9 minutes to obtain liposome colostrum;

[0062] (4) The liposome colostrum was homogenized under high pressure for 3 times at 500 bar to obtain an essence containing osmanthus extract having soothing, firming and anti-wrinkle properties.

[0063] Comparative Example 1

[0064] This comparative example refers to the components and preparation method in Example 1, and the only difference from Example 1 is that this comparative example omits the frankincense seed extract; the amount of frankincense seed extract missing is distributed to the corresponding components according to the ratio of osmanthus extract, betaine, and pentapeptide. The remaining steps are carried out in accordance with Example 1.

[0065] Comparative Example 2

[0066] This comparative example refers to the components and preparation method in Example 1, and the only difference from Example 1 is that betaine is omitted in this comparative example; the missing amount of betaine is distributed to the corresponding components according to the ratio of osmanthus extract, frankincense seed extract, and pentapeptide. The remaining steps are carried out with reference to Example 1.

[0067] Comparative Example 3

[0068] This comparative example refers to the components and preparation method in Example 1, and the only difference from Example 1 is that the present comparative example omits the pentapeptide; the missing amount of the pentapeptide is distributed to the corresponding components according to the ratio of osmanthus extract, betaine, and frankincense seed extract. The remaining steps are carried out in accordance with Example 1.

[0069] Comparative Example 4

[0070] This comparative example refers to the components in Example 1, and the only difference from Example 1 is that the preparation method of the essence in this comparative example includes the following steps:

[0071] According to the ratio in Table 1, each component was weighed, mixed, heated to 50° C., and sheared at 1000 rpm for 20 min to obtain an essence.

[0072] Test Example 1

[0073] This test example explores the anti-inflammatory effect of the essence obtained in the example and the comparative example, with specific reference to T / SHRH033-2020 "Cosmetic Soothing Efficacy Test-In Vitro TNF-α Inflammatory Factor Content Determination Lipopolysaccharide-Induced Macrophage RAW264.7 Test Method", the added concentration of LPS is 1 mg / mL, and the test content of the essence is 10wt%. The test results are shown in Table 2.

[0074] Table 2

[0075]

[0076] It can be seen from Table 2 that, compared with the comparative example, the essence provided by the embodiment of the present invention can significantly inhibit the inflammatory factor TNF-α.

[0077] Test Example 2

[0078] This test example explores the promoting effect of the essence obtained in the examples and comparative examples on collagen secretion, with specific reference to T / SHRH 031 2020 "Determination of type I collagen content in fibroblasts in vitro for cosmetic firming and anti-wrinkle efficacy test". The test content of the essence is 10wt%, and the test results are shown in Table 3.

[0079] Table 3

[0080] Group Relative content of type Ⅰ collagen (%) Blank Group 99.2 Example 1 161.5 Example 2 168.1 Example 3 164.9 Comparative Example 1 137.5 Comparative Example 2 132.3 Comparative Example 3 139.7 Comparative Example 4 133.4

[0081] It can be seen from Table 3 that, compared with the comparative example, the essence provided by the embodiment of the present invention can significantly promote the secretion of type I collagen by fibroblasts.

[0082] Test Example 3

[0083] Matrix metalloproteinases (MMPs) are a class of proteolytic enzymes that can degrade extracellular matrices (such as collagen, elastin, proteoglycans, etc.). Matrix metalloproteinase 1 (MMP-1) is a member of the MMP family that mainly degrades type I collagen. The content of MMP-1 in normal dormant tissues is extremely low, but ultraviolet radiation promotes MMP-1 expression, increases abnormal collagen degradation, and leads to reduced skin strength and elasticity, resulting in skin aging.

[0084] This test example explores the inhibitory effect of the essence obtained in the examples and comparative examples on matrix metalloproteinases, and specifically includes the following steps:

[0085] (1) Human skin fibroblasts (HSF) cells in good exponential growth phase were taken and digested with 0.25% trypsin solution to make the attached cells fall off. Count 1×10 6 / mL to prepare a cell suspension.

[0086] (2) Dilute appropriately to 10 5 The cell suspension was inoculated on a 6-well plate until the cells grew to about 80% to establish a UV photoaging model. A blank control group was set up: 200 μL PBS, supplemented with culture medium to 800 μL, without UV irradiation; UV group: UV irradiation, plus PBS; experimental group: essence obtained in the embodiment and comparative example (1wt%).

[0087] (3) The UV group and the experimental group were washed repeatedly with appropriate amount of PBS until colorless, and then 200 μL PBS was added and placed at 80 mJ / cm 2 Irradiate under UV light, with the distance between the light source and the culture bottle being 15 cm. After irradiation, discard PBS, add PBS solution and culture medium to 800 μL in the UV group, and add culture medium and the sample to be tested to 800 μL in the experimental group; the blank control group, UV group, and experimental group continue to incubate in the incubator for 48 h.

[0088] (4) Scrape the cells, mix them by pipetting, extract the cell proteins by RIPA, quantify them by BCA to 1 mg / mL, and operate according to the instructions of matrix metalloproteinase 1 ELISA. Measure the OD value of each well at 450 nm by using a microplate reader within 15 minutes. The experimental results are shown in Table 4.

[0089] Table 4

[0090] Group MMP-1 (pg / mg) Blank control group 54.3 UV Groups 210.7 Example 1 136.9 Example 2 131.5 Example 3 132.6 Comparative Example 1 175.2 Comparative Example 2 180.5 Comparative Example 3 184.3 Comparative Example 4 177.9

[0091] It can be seen from Table 4 that, compared with the comparative example, the essence provided by the embodiment of the present invention can significantly reduce the content of MMP-1, thereby reducing the degradation of collagen.

[0092] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. An essence containing osmanthus extract with soothing, firming and anti-wrinkle and lightening effects, characterized in that: The composition comprises, by weight: 10 to 30 parts of osmanthus extract, 2 to 10 parts of frankincense seed extract, 2 to 10 parts of betaine, 2 to 10 parts of pentapeptide, 1 to 5 parts of hydrogenated soybean lecithin, 1 to 5 parts of xylitol, and 60 to 80 parts of water; the solid content of the osmanthus extract is 3 to 5 mg / mL; and also comprises disodium EDTA and phenoxyethanol; The preparation method of the osmanthus extract comprises: The osmanthus flowers are crushed, mixed with water, and distilled under reduced pressure to obtain a distillate, and the distillate is concentrated to a solid content of 3 to 5 mg / mL to obtain an osmanthus flower extract; The solid-liquid ratio of the osmanthus to water is 1:(10-20) g / mL; the temperature of the reduced pressure distillation is 80-90° C., the time is 2-3 hours, and the pressure is -0.09--0.1 MPa; The preparation method of the frankincense seed extract comprises: The frankincense seeds are mixed with ethanol, extracted at 50-60°C for 1-2 hours, and concentrated under reduced pressure; the obtained product is freeze-dried to obtain the frankincense seeds extract; The preparation method of the essence containing osmanthus extract with soothing, firming and anti-wrinkle and lightening effects comprises: (1) mixing osmanthus extract, frankincense seed extract, betaine, pentapeptide and water, and heating to 50° C. to obtain an aqueous phase; Mix hydrogenated soybean lecithin, disodium EDTA, xylitol and phenoxyethanol, and heat to 50° C. to obtain an oil phase; (2) mixing the oil phase and the water phase, and shearing for 5 to 15 minutes at 10,000 to 12,000 rpm to obtain liposome colostrum; (3) The liposome colostrum is homogenized under high pressure at 500 to 600 bar to obtain an essence containing osmanthus extract having soothing, firming and anti-wrinkle and line-reducing effects.

2. The essence containing osmanthus extract with soothing, firming and anti-wrinkle and lightening properties according to claim 1, characterized in that: In parts by mass, including: 10 parts of osmanthus extract, 6 parts of frankincense seed extract, 4 parts of betaine, 5 parts of pentapeptide, 2 parts of hydrogenated soybean lecithin, 2 parts of xylitol, 0.5 parts of disodium EDTA, 1 part of phenoxyethanol, 69.5 parts of water; Alternatively, the essence containing osmanthus extract having the effects of soothing, firming and anti-wrinkle and lightening lines comprises, by parts by mass: 20 parts of osmanthus extract, 5 parts of frankincense seed extract, 3 parts of betaine, 4 parts of pentapeptide, 2 parts of hydrogenated soybean lecithin, 2 parts of xylitol, 0.5 parts of disodium EDTA, 1 part of phenoxyethanol, 62.5 parts of water; Alternatively, the essence containing osmanthus extract having the effects of soothing, firming and anti-wrinkle and lightening lines comprises, by parts by mass: 15 parts of osmanthus extract, 4 parts of frankincense seed extract, 3 parts of betaine, 6 parts of pentapeptide, 2 parts of hydrogenated soybean lecithin, 2 parts of xylitol, 0.5 parts of disodium EDTA, 1 part of phenoxyethanol, and 66.5 parts of water.

3. Use of the essence containing osmanthus extract with soothing, firming and anti-wrinkle and line-lightening effects as claimed in claim 1 or claim 2 in the preparation of anti-inflammatory products.

4. Use of the essence containing osmanthus extract with soothing, firming and anti-wrinkle and line-lightening effects as claimed in claim 1 or claim 2 in the preparation of anti-aging products.

5. Use of the essence containing osmanthus fragrans extract with soothing, firming and anti-wrinkle and line-lightening effects as claimed in claim 1 or claim 2 in the preparation of products for reducing the content of matrix metalloproteinases and / or promoting collagen secretion.

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