A composite plant growth regulator for promoting rooting of tea tree cuttings and improving survival rate and its application

Abstract

The present invention belongs to the technical field of plant growth regulators. More specifically, the present invention provides a composite plant growth regulator for tea tree cuttings, comprising a composition A and a composition B used separately; the composition A comprises ascorbic acid, brassinolide, indolebutyric acid, eugenol and diosgenin, and the composition B comprises furifrinol, diethylaminoethanol hexanoate, aluminum sulfate, ammonium dihydrogen phosphate and zinc sulfate. The composite plant growth regulator of the present invention effectively reduces the formation of non-rooting callus at the base of the cuttings, and significantly increases the number and length of adventitious roots. In addition, the present invention can not only significantly improve the number and quality of the root system of the cuttings, improve the rooting rate of the cuttings, but also effectively reduce the cost of tea tree seedling cultivation, greatly reduce the loss and cycle of tea tree seedling cultivation, and provide strong technical support for the large-scale cutting propagation of difficult-to-root tea trees, and has good application prospects and value.

Description

Technical Field

[0001] The present invention belongs to the technical field of plant growth regulators, and more specifically, relates to a composite plant growth regulator for promoting the rooting of tea tree cuttings and improving the survival rate, and its application. Background Art

[0002] Tea is one of the three major beverages in the world. Tea is brewed from tea leaves, which are refined from the leaves and buds of the tea tree. The tea tree is a plant of the genus Camellia in the family Theaceae. Currently, cuttings are one of the most widely used methods for tea tree variety breeding. Compared with other asexual propagation methods, cutting propagation technology has the advantages of simple operation, high propagation coefficient, and the ability to maintain the excellent traits of the mother plant.

[0003] The key to tea tree cutting propagation lies in the formation of adventitious roots. However, tea tree cutting propagation generally has problems such as low cutting rooting rate, small number of roots, and low cutting survival rate, especially for some difficult-to-root tea trees, such as Hainan large-leaf tea.

[0004] Rooting agents are auxin compounds that belong to the class of plant growth regulators and promoters. Their types and concentrations have a great influence on the formation of adventitious roots of tea tree cuttings. Some rooting agents currently on the market will induce a large amount of callus tissue while inducing rooting in tea trees. However, the number of adventitious roots growing from the callus tissue is very small, and the rooting time is slow. At the same time, the gradual lignification of the callus tissue will also lead to the failure of rooting of the cuttings, especially some cuttings with overly developed callus tissue, which almost do not grow roots or grow very few roots, and therefore cannot be used for rooting difficult-to-root tea trees.

[0005] Therefore, developing products that can promote the rooting of tea tree cuttings and increase their survival rate, especially developing products that promote the rooting of cuttings for difficult-to-root tea trees, increasing the rooting rate and survival rate of cuttings and reducing the cost of tea tree seedling cultivation, is of great significance to the development of the tea industry. Summary of the invention

[0006] The invention aims to overcome the problems of low rooting rate and low survival rate of cuttings in tea tree cuttings, and provides a composite plant growth regulator for tea tree cuttings.

[0007] The first object of the present invention is to provide a composite plant growth regulator for tea tree cuttings.

[0008] The second object of the present invention is to provide the application of the above-mentioned composite plant growth regulator.

[0009] The third object of the present invention is to provide a method for tea tree cutting seedling cultivation.

[0010] The above-mentioned purpose of the present invention is achieved through the following technical solutions:

[0011] The invention provides a composite plant growth regulator for tea tree cuttings, comprising a composition A and a composition B which are used separately; the composition A comprises ascorbic acid, brassinolide, indolebutyric acid, eugenol and diosgenin, and the composition B comprises furifrinol, diethylaminoethanol caproate, aluminum sulfate, ammonium dihydrogen phosphate and zinc sulfate.

[0012] Preferably, the mass ratio of ascorbic acid, brassinolide, indolebutyric acid, eugenol and diosgenin in composition A is (5-25): (0.5-1.8): (80-250): (3-12): (0.6-2.5).

[0013] More preferably, the mass ratio of ascorbic acid, brassinolide, indolebutyric acid, eugenol and diosgenin in composition A is (12-16): (0.8-1.5): (140-200): (7-9): (1.2-1.8).

[0014] Further preferably, the mass ratio of ascorbic acid, brassinolide, indolebutyric acid, eugenol and diosgenin in composition A is 15:1.2:160:8:1.5.

[0015] Preferably, the mass ratio of flurprimidol, diethylaminoethanol hexanoate, aluminum sulfate, diammonium phosphate and zinc sulfate in composition B is (2-10): (10-18): (40-80): (10-50): (0.5-2).

[0016] More preferably, the mass ratio of flurprimidol, diethylaminoethanol hexanoate, aluminum sulfate, diammonium phosphate and zinc sulfate in composition B is (5-7): (12-16): (50-70): (20-40): (1.0-1.5).

[0017] Further preferably, the mass ratio of furifrinol, diethylaminoethanol hexanoate, aluminum sulfate, diammonium dihydrogen phosphate and zinc sulfate in composition B is 6:14:60:30:1.3.

[0018] As an optional embodiment, the above-mentioned composite plant growth regulator includes a composition A solution and a composition B solution; the composition A solution includes the following components in mass concentrations: 5~25 mg / L ascorbic acid, 0.5~1.8 mg / L brassinolide, 80~250 mg / L indolebutyric acid, 3~12 mg / L eugenol and 0.6~2.5 mg / L diosgenin.

[0019] As an optional embodiment, the above-mentioned composite plant growth regulator includes a composition A solution and a composition B solution; the composition B solution includes the following components in mass concentrations: 2~10 mg / L furaporidine, 10~18 mg / L diethylaminoethanol hexanoate, 40~80 mg / L aluminum sulfate, 10~50 mg / L diammonium phosphate and 0.5~2 mg / L zinc sulfate.

[0020] As an optional embodiment, the above-mentioned composite plant growth regulator includes a composition A solution and a composition B solution;

[0021] The composition A solution includes the following components in mass concentrations: 15 mg / L ascorbic acid, 1.2 mg / L brassinolide, 160 mg / L indolebutyric acid, 8 mg / L eugenol, and 1.5 mg / L diosgenin;

[0022] The composition B solution includes the following components in mass concentrations: 6 mg / L furifrimol, 14 mg / L diethylaminoethanol hexanoate, 60 mg / L aluminum sulfate, 30 mg / L diammonium dihydrogen phosphate, and 1.3 mg / L zinc sulfate.

[0023] The present invention has obtained a composite plant growth regulator, with ascorbic acid, brassinolide, indolebutyric acid, eugenol, and diosgenin as composition A, and furifrinol, diethylaminoethanol caproate, aluminum sulfate, ammonium dihydrogen phosphate, and zinc sulfate as composition B. During the cutting process, through the synergistic effect of the components in composition A and composition B, not only the callus formation at the base of the cuttings is effectively reduced, the number and length of adventitious roots are significantly increased, and the rooting time is accelerated. In addition, the composite plant growth regulator of the present invention has a significant improvement in the rooting rate and survival rate of the cuttings, greatly improving the success rate of tea tree cutting propagation. Therefore, the present invention provides the following application scheme:

[0024] The application of the composite plant growth regulator in promoting the rooting of tea tree cuttings.

[0025] Application of the composite plant growth regulator in the preparation of products for promoting rooting of cuttings.

[0026] The application of the above-mentioned composite plant growth regulator in improving the rooting rate of cuttings.

[0027] The composite plant growth regulator is used in preparing a product for improving the rooting rate of cuttings.

[0028] Application of the above-mentioned composite plant growth regulator in increasing the number of adventitious roots of cuttings.

[0029] The composite plant growth regulator is used in preparing a product for increasing the number of adventitious roots of cuttings.

[0030] Application of the composite plant growth regulator in increasing the length of adventitious roots of cuttings.

[0031] The composite plant growth regulator is used in preparing a product for increasing the length of adventitious roots of cuttings.

[0032] Application of the above-mentioned composite plant growth regulator in reducing callus formation of cuttings.

[0033] Application of the composite plant growth regulator in the preparation of a product for reducing callus formation of cuttings.

[0034] The application of the composite plant growth regulator in improving the survival rate of tea tree cuttings.

[0035] The application of the composite plant growth regulator in the preparation of products for improving the survival rate of cuttings.

[0036] The above-mentioned composite plant growth regulator is used in tea tree cuttings, especially in tea tree cuttings that are difficult to root.

[0037] The present invention also provides a method for tea tree cutting seedling cultivation, wherein the tea tree cuttings are soaked in the solution of the composition A for 12 to 16 hours and then cut, and after 5 to 10 days of cutting, the solution of the composition B is applied to the leaf surface of the cuttings.

[0038] As an alternative embodiment, the method is to soak the base 1-3 cm of the cuttings in the above-mentioned composition A solution.

[0039] As an optional embodiment, the brassinolide, indolebutyric acid, eugenol and diosgenin components in composition A use anhydrous ethanol as solvent, and the ascorbic acid component uses water as solvent.

[0040] As an alternative embodiment, the furaporidine and diethylaminoethanol hexanoate components in composition B use anhydrous ethanol as solvent, and the aluminum sulfate, ammonium dihydrogen phosphate and zinc sulfate components use water as solvent.

[0041] The present invention has the following beneficial effects:

[0042] 1. The present invention provides a composite plant growth regulator, with ascorbic acid, brassinolide, indolebutyric acid, eugenol, and diosgenin as composition A, and furifrinol, diethylaminoethanol hexanoate, aluminum sulfate, ammonium dihydrogen phosphate, and zinc sulfate as composition B. In the process of cutting, through the synergistic effect of the components in composition A and composition B, not only the callus formation at the base of the cuttings is effectively reduced, the number and length of adventitious roots are significantly increased, and the rooting time is accelerated. The test results show that compared with the existing rooting agent, the composite plant growth regulator of the present invention has a significant improvement in the rooting rate and survival rate of the cuttings, greatly improving the success rate of tea tree cutting propagation.

[0043] 2. The unique ratio of furifrinol, diethylaminoethanol hexanoate, aluminum sulfate, ammonium dihydrogen phosphate and zinc sulfate in the composition B of the present invention is very important for the rooting process of the cuttings. Experimental data show that the root system of the cuttings lacking composition B is retarded, the number and quality of roots are insufficient, and the rooting and root system development in the later stage are obviously inferior to the cuttings using composition B. This further proves that composition B promotes the strong growth of the root system by regulating the growth balance of the above-ground and underground parts of the plant, and provides the necessary nutritional support for the formation and extension of adventitious roots.

[0044] 3. The present invention optimizes the hormone balance and nutrient supply of cuttings by scientifically optimizing the ingredient ratio of compositions A and B, and promotes the formation of root primordia and the rapid growth of adventitious roots through the supplementation of exogenous hormones, thereby improving the rooting effect of tea tree cuttings.

[0045] 4. Compared with traditional rooting agents, the present invention not only shortens the time of tea tree cutting seedling cultivation, but also significantly improves the quantity and quality of the root system of the cuttings, and improves the rooting rate of the cuttings. The present invention can effectively reduce the cost of tea tree seedling cultivation, greatly reduce the loss and cycle of tea tree seedling cultivation, and provide strong technical support for the large-scale cutting propagation of difficult-to-root tea trees. BRIEF DESCRIPTION OF THE DRAWINGS

[0046] Figure 1 The figure shows the rooting conditions of the cuttings treated in different embodiments and comparative examples after 60 days of cutting.

[0047] Figure 2 The figure shows the rooting conditions of the cuttings treated in different embodiments and comparative examples after 120 days of cutting.

[0048] Figure 3 The following is a comparison of the growth of tea trees in the field of Example 1 and Comparative Example 4.

[0049] Figure 4 The figure is a comparison of the growth of tea trees in the field of Example 2 and Comparative Example 5.

[0050] Figure 5 The figure is a comparison of the growth of tea trees in the field of Example 3 and Comparative Example 8. DETAILED DESCRIPTION

[0051] The present invention is further described below in conjunction with the accompanying drawings and specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0052] Unless otherwise specified, the reagents and materials used in the following examples are commercially available.

[0053] The tea trees used for cuttings in the following embodiments are difficult-to-root tea trees, and the Hainan large-leaf tea variety is used as an example in the embodiments.

[0054] Ascorbic acid, CAS: 50-81-7, structural formula: .

[0055] Brassinolide, CAS: 72962-43-7, structural formula: .

[0056] Indolebutyric acid, CAS: 133-32-4, structural formula: .

[0057] Eugenol, CAS: 97-53-0, structural formula: .

[0058] Diosgenin, CAS: 512-06-1, structural formula:

[0059] .

[0060] Flurprimidol, CAS: 56425-91-3, structural formula: .

[0061] Diethylaminoethanol hexanoate, CAS: 10369-83-2, structural formula:

[0062] .

[0063] Example 1

[0064] A composite plant growth regulator for tea tree cuttings comprises a composition A and a composition B.

[0065] Composition A comprises ascorbic acid, brassinolide, indolebutyric acid, eugenol and diosgenin, wherein the mass ratio of ascorbic acid, brassinolide, indolebutyric acid, eugenol and diosgenin is 5:0.5:80:3:0.6;

[0066] Composition B includes flurprimidol, diethylaminoethanol hexanoate, aluminum sulfate, ammonium dihydrogen phosphate and zinc sulfate, and the mass ratio of flurprimidol, diethylaminoethanol hexanoate, aluminum sulfate, ammonium dihydrogen phosphate and zinc sulfate is 2:10:40:10:0.5.

[0067] Composition A solution preparation method:

[0068] First, 0.5 mg of brassinolide, 80 mg of indolebutyric acid, 3 mg of eugenol and 0.6 mg of diosgenin were weighed and completely dissolved in 50 mL of anhydrous ethanol to obtain a mixed solution 1. Then 5 mg of ascorbic acid was weighed and dissolved in 20 mL of water to obtain a mixed solution 2. Mixed solutions 1 and 2 were mixed together, and the volume was adjusted to 1 L with water to prepare a composition A solution, wherein the concentrations of ascorbic acid, brassinolide, indolebutyric acid, eugenol and diosgenin were 5 mg / L, 0.5 mg / L, 80 mg / L, 3 mg / L and 0.6 mg / L, respectively.

[0069] Preparation method of composition B solution:

[0070] First, 40 mg of aluminum sulfate, 10 mg of ammonium dihydrogen phosphate, and 0.5 mg of zinc sulfate were weighed and dissolved in 100 mL of water to obtain a mixed solution 1. Then, 2 mg of flurprimidol and 10 mg of diethylaminoethanol hexanoate were weighed and dissolved in 50 mL of anhydrous ethanol to obtain a mixed solution 2. Mixed solutions 1 and 2 were mixed together, and the volume was adjusted to 1 L with water to prepare a composition B solution, in which the concentrations of flurprimidol, diethylaminoethanol hexanoate, aluminum sulfate, ammonium dihydrogen phosphate, and zinc sulfate were 2 mg / L, 10 mg / L, 40 mg / L, 10 mg / L, and 0.5 mg / L, respectively.

[0071] Example 2

[0072] A composite plant growth regulator for tea tree cuttings comprises a composition A and a composition B.

[0073] Composition A comprises ascorbic acid, brassinolide, indolebutyric acid, eugenol and diosgenin, wherein the mass ratio of ascorbic acid, brassinolide, indolebutyric acid, eugenol and diosgenin is 15:1.2:160:8:1.5;

[0074] Composition B includes flurprimidol, diethylaminoethanol hexanoate, aluminum sulfate, ammonium dihydrogen phosphate and zinc sulfate, and the mass ratio of flurprimidol, diethylaminoethanol hexanoate, aluminum sulfate, ammonium dihydrogen phosphate and zinc sulfate is 6:14:60:30:1.3.

[0075] Composition A solution preparation method:

[0076] First, 1.2 mg of brassinolide, 160 mg of indolebutyric acid, 8 mg of eugenol and 1.5 mg of diosgenin were weighed and completely dissolved in 50 mL of anhydrous ethanol to obtain a mixed solution 1. Then 15 mg of ascorbic acid was weighed and dissolved in 20 mL of water to obtain a mixed solution 2. Mixed solutions 1 and 2 were mixed together, and the volume was adjusted to 1 L with water to prepare a composition A solution, wherein the concentrations of ascorbic acid, brassinolide, indolebutyric acid, eugenol and diosgenin were 15 mg / L, 1.2 mg / L, 160 mg / L, 8 mg / L and 1.5 mg / L, respectively.

[0077] Preparation method of composition B solution:

[0078] First, 60 mg of aluminum sulfate, 30 mg of ammonium dihydrogen phosphate, and 1.3 mg of zinc sulfate were weighed and dissolved in 100 mL of water to obtain a mixed solution 1. Then, 6 mg of flurprimidol and 14 mg of diethylaminoethanol hexanoate were weighed and dissolved in 50 mL of anhydrous ethanol to obtain a mixed solution 2. Mixed solutions 1 and 2 were mixed together, and the volume was adjusted to 1 L with water to prepare a composition B solution, in which the concentrations of flurprimidol, diethylaminoethanol hexanoate, aluminum sulfate, ammonium dihydrogen phosphate, and zinc sulfate were 6 mg / L, 14 mg / L, 60 mg / L, 30 mg / L, and 1.3 mg / L, respectively.

[0079] Example 3

[0080] A composite plant growth regulator for tea tree cuttings comprises a composition A and a composition B.

[0081] Composition A comprises ascorbic acid, brassinolide, indolebutyric acid, eugenol and diosgenin, wherein the mass ratio of ascorbic acid, brassinolide, indolebutyric acid, eugenol and diosgenin is 25:1.8:250:12:2.5;

[0082] Composition B includes flurprimidol, diethylaminoethanol hexanoate, aluminum sulfate, ammonium dihydrogen phosphate and zinc sulfate, and the mass ratio of flurprimidol, diethylaminoethanol hexanoate, aluminum sulfate, ammonium dihydrogen phosphate and zinc sulfate is 10:18:80:50:2.

[0083] Composition A solution preparation method:

[0084] First, 1.8 mg of brassinolide, 250 mg of indolebutyric acid, 12 mg of eugenol and 2.5 mg of diosgenin were weighed and completely dissolved in 50 mL of anhydrous ethanol to obtain a mixed solution 1. Then 25 mg of ascorbic acid was weighed and dissolved in 20 mL of water to obtain a mixed solution 2. Mixed solutions 1 and 2 were mixed together, and the volume was adjusted to 1 L with water to prepare a composition A solution, wherein the concentrations of ascorbic acid, brassinolide, indolebutyric acid, eugenol and diosgenin were 25 mg / L, 1.8 mg / L, 250 mg / L, 12 mg / L and 2.5 mg / L, respectively.

[0085] Preparation method of composition B solution:

[0086] First, 80 mg of aluminum sulfate, 50 mg of ammonium dihydrogen phosphate, and 2 mg of zinc sulfate were weighed and dissolved in 100 mL of water to obtain a mixed solution 1. Then, 10 mg of flurprimidol and 18 mg of diethylaminoethanol hexanoate were weighed and dissolved in 50 mL of anhydrous ethanol to obtain a mixed solution 2. Mixed solutions 1 and 2 were mixed together, and the volume was adjusted to 1 L with water to prepare a composition B solution, in which the concentrations of flurprimidol, diethylaminoethanol hexanoate, aluminum sulfate, ammonium dihydrogen phosphate, and zinc sulfate were 10 mg / L, 18 mg / L, 80 mg / L, 50 mg / L, and 2 mg / L, respectively.

[0087] Comparative Example 1

[0088] The composition A solution and the composition B solution were both replaced with clean water.

[0089] Comparative Example 2

[0090] The solution of composition A is ABT-1 rooting powder solution (1 g / L): weigh 1 g of ABT-1 rooting powder, put it in a beaker, add 50 mL of anhydrous ethanol until the rooting powder is completely dissolved, and then add water to make up to 1 L.

[0091] The composition B solution was replaced with clean water.

[0092] Comparative Example 3

[0093] Composition A solution is indolebutyric acid solution (150 mg / L): weigh 150 mg of indolebutyric acid, put it in a beaker, add 30 mL of anhydrous ethanol to dissolve it, and then add water to make up to 1 L.

[0094] The composition B solution was replaced with clean water.

[0095] Comparative Example 4

[0096] A composite plant growth regulator comprising composition A only.

[0097] Composition A comprises ascorbic acid, brassinolide, indolebutyric acid, eugenol and diosgenin, and the mass ratio of ascorbic acid, brassinolide, indolebutyric acid, eugenol and diosgenin is 15:1.2:160:8:1.5.

[0098] Preparation method of composition A solution:

[0099] First, 1.2 mg of brassinolide, 160 mg of indolebutyric acid, 8 mg of eugenol and 1.5 mg of diosgenin were weighed and completely dissolved in 50 mL of anhydrous ethanol to obtain a mixed solution 1. Then 15 mg of ascorbic acid was weighed and dissolved in 20 mL of water to obtain a mixed solution 2. Mixed solutions 1 and 2 were mixed together, and the volume was adjusted to 1 L with water to prepare a composition A solution, wherein the concentrations of ascorbic acid, brassinolide, indolebutyric acid, eugenol and diosgenin were 15 mg / L, 1.2 mg / L, 160 mg / L, 8 mg / L and 1.5 mg / L, respectively.

[0100] The composition B solution was replaced with clean water.

[0101] Comparative Example 5

[0102] A composite plant growth regulator comprises a composition A and a composition B.

[0103] Composition A comprises ascorbic acid, brassinolide, indolebutyric acid and diosgenin, wherein the mass ratio of ascorbic acid, brassinolide, indolebutyric acid and diosgenin is 15:1.2:160:1.5;

[0104] Composition B includes flurprimidol, diethylaminoethanol hexanoate, aluminum sulfate, ammonium dihydrogen phosphate and zinc sulfate, and the mass ratio of flurprimidol, diethylaminoethanol hexanoate, aluminum sulfate, ammonium dihydrogen phosphate and zinc sulfate is 6:14:60:30:1.3.

[0105] Composition A solution preparation method:

[0106] First, 1.2 mg of brassinolide, 160 mg of indolebutyric acid and 1.5 mg of diosgenin were weighed and completely dissolved in 50 mL of anhydrous ethanol to obtain a mixed solution 1. Then 15 mg of ascorbic acid was weighed and dissolved in 20 mL of water to obtain a mixed solution 2. Mixed solutions 1 and 2 were mixed together and fixed to 1 L to prepare a composition A solution, wherein the concentrations of ascorbic acid, brassinolide, indolebutyric acid and diosgenin were 15 mg / L, 1.2 mg / L, 160 mg / L and 1.5 mg / L, respectively.

[0107] Preparation method of composition B solution:

[0108] First, 60 mg of aluminum sulfate, 30 mg of ammonium dihydrogen phosphate, and 1.3 mg of zinc sulfate were weighed and dissolved in 100 mL of water to obtain a mixed solution 1. Then, 6 mg of flurprimidol and 14 mg of diethylaminoethanol hexanoate were weighed and dissolved in 50 mL of anhydrous ethanol to obtain a mixed solution 2. Mixed solutions 1 and 2 were mixed together, and then diluted to 1 L with water to prepare a composition B solution, in which the concentrations of flurprimidol, diethylaminoethanol hexanoate, aluminum sulfate, ammonium dihydrogen phosphate, and zinc sulfate were 6 mg / L, 14 mg / L, 60 mg / L, 30 mg / L, and 1.3 mg / L, respectively.

[0109] Comparative Example 6

[0110] A composite plant growth regulator comprises a composition A and a composition B.

[0111] Composition A comprises ascorbic acid, brassinolide, indolebutyric acid and eugenol, wherein the mass ratio of ascorbic acid, brassinolide, indolebutyric acid and eugenol is 15:1.2:160:8;

[0112] Composition B includes flurprimidol, diethylaminoethanol hexanoate, aluminum sulfate, ammonium dihydrogen phosphate and zinc sulfate, and the mass ratio of flurprimidol, diethylaminoethanol hexanoate, aluminum sulfate, ammonium dihydrogen phosphate and zinc sulfate is 6:14:60:30:1.3.

[0113] Preparation method:

[0114] Composition A solution preparation method:

[0115] First, 1.2 mg of brassinolide, 160 mg of indolebutyric acid and 8 mg of eugenol were weighed and completely dissolved in 50 mL of anhydrous ethanol to obtain a mixed solution 1. Then 15 mg of ascorbic acid was weighed and dissolved in 20 mL of water to obtain a mixed solution 2. Mixed solutions 1 and 2 were mixed together and fixed to 1 L to prepare a composition A solution, wherein the concentrations of ascorbic acid, brassinolide, indolebutyric acid and eugenol were 15 mg / L, 1.2 mg / L, 160 mg / L and 8 mg / L, respectively.

[0116] Preparation method of composition B solution:

[0117] First, 60 mg of aluminum sulfate, 30 mg of ammonium dihydrogen phosphate, and 1.3 mg of zinc sulfate were weighed and dissolved in 100 mL of water to obtain a mixed solution 1. Then, 6 mg of flurprimidol and 14 mg of diethylaminoethanol hexanoate were weighed and dissolved in 50 mL of anhydrous ethanol to obtain a mixed solution 2. Mixed solutions 1 and 2 were mixed together, and then diluted to 1 L with water to prepare a composition B solution, in which the concentrations of flurprimidol, diethylaminoethanol hexanoate, aluminum sulfate, ammonium dihydrogen phosphate, and zinc sulfate were 6 mg / L, 14 mg / L, 60 mg / L, 30 mg / L, and 1.3 mg / L, respectively.

[0118] Comparative Example 7

[0119] A composite plant growth regulator for tea tree cuttings comprises a composition A and a composition B.

[0120] Composition A comprises ascorbic acid, brassinolide, indolebutyric acid, eugenol and diosgenin, wherein the mass ratio of ascorbic acid, brassinolide, indolebutyric acid, eugenol and diosgenin is 15:1.2:160:8:1.5;

[0121] Composition B includes chlormequat chloride, diethylaminoethanol caproate, aluminum sulfate, ammonium dihydrogen phosphate and zinc sulfate, and the mass ratio of chlormequat chloride, diethylaminoethanol caproate, aluminum sulfate, ammonium dihydrogen phosphate and zinc sulfate is 6:14:60:30:1.3.

[0122] Composition A solution preparation method:

[0123] First, 1.2 mg of brassinolide, 160 mg of indolebutyric acid, 8 mg of eugenol and 1.5 mg of diosgenin were weighed and completely dissolved in 50 mL of anhydrous ethanol to obtain a mixed solution 1. Then 15 mg of ascorbic acid was weighed and dissolved in 20 mL of water to obtain a mixed solution 2. Mixed solutions 1 and 2 were mixed together, and the volume was adjusted to 1 L with water to prepare a composition A solution, wherein the concentrations of ascorbic acid, brassinolide, indolebutyric acid, eugenol and diosgenin were 15 mg / L, 1.2 mg / L, 160 mg / L, 8 mg / L and 1.5 mg / L, respectively.

[0124] Preparation method of composition B solution:

[0125] First, 6 mg of chlormequat, 60 mg of aluminum sulfate, 30 mg of ammonium dihydrogen phosphate, and 1.3 mg of zinc sulfate were weighed and dissolved in 100 mL of water to obtain a mixed solution 1. Then 14 mg of diethylaminoethanol hexanoate was weighed and dissolved in 50 mL of anhydrous ethanol to obtain a mixed solution 2. Mixed solutions 1 and 2 were mixed together, and the volume was adjusted to 1 L with water to prepare a composition B solution, in which the concentrations of chlormequat, diethylaminoethanol hexanoate, aluminum sulfate, ammonium dihydrogen phosphate, and zinc sulfate were 6 mg / L, 14 mg / L, 60 mg / L, 30 mg / L, and 1.3 mg / L, respectively.

[0126] Comparative Example 8

[0127] A composite plant growth regulator for tea tree cuttings comprises a composition A and a composition B.

[0128] Composition A comprises ascorbic acid, brassinolide, indolebutyric acid, eugenol and diosgenin, wherein the mass ratio of ascorbic acid, brassinolide, indolebutyric acid, eugenol and diosgenin is 15:1.2:160:8:1.5;

[0129] The composition B comprises uniconazole, diethylaminoethanol caproate, aluminum sulfate, ammonium dihydrogen phosphate and zinc sulfate, and the mass ratio of uniconazole, diethylaminoethanol caproate, aluminum sulfate, ammonium dihydrogen phosphate and zinc sulfate is 6:14:60:30:1.3.

[0130] Composition A solution preparation method:

[0131] First, 1.2 mg of brassinolide, 160 mg of indolebutyric acid, 8 mg of eugenol and 1.5 mg of diosgenin were weighed and completely dissolved in 50 mL of anhydrous ethanol to obtain a mixed solution 1. Then 15 mg of ascorbic acid was weighed and dissolved in 20 mL of water to obtain a mixed solution 2. Mixed solutions 1 and 2 were mixed together, and the volume was adjusted to 1 L with water to prepare a composition A solution, wherein the concentrations of ascorbic acid, brassinolide, indolebutyric acid, eugenol and diosgenin were 15 mg / L, 1.2 mg / L, 160 mg / L, 8 mg / L and 1.5 mg / L, respectively.

[0132] Preparation method of composition B solution:

[0133] First, 60 mg of aluminum sulfate, 30 mg of ammonium dihydrogen phosphate, and 1.3 mg of zinc sulfate were weighed and dissolved in 100 mL of water to obtain a mixed solution 1. Then, 6 mg of vinconazole and 14 mg of diethylaminoethanol hexanoate were weighed and dissolved in 50 mL of anhydrous ethanol to obtain a mixed solution 2. Mixed solutions 1 and 2 were mixed together, and the volume was adjusted to 1 L with water to prepare a composition B solution, in which the concentrations of vinconazole, diethylaminoethanol hexanoate, aluminum sulfate, ammonium dihydrogen phosphate, and zinc sulfate were 6 mg / L, 14 mg / L, 60 mg / L, 30 mg / L, and 1.3 mg / L, respectively.

[0134] Experimental Example 1

[0135] 1. Experimental Methods

[0136] (1) Cutting method

[0137] The solutions of Examples 1-3 and Comparative Examples 1-8 were used for cuttings of the difficult-to-root tea tree Hainan large-leaf tea. The specific cutting method was as follows:

[0138] Immerse 1-3 cm of the base of the cuttings in the solution of composition A prepared in each example or comparative example for 12 h, then take out and directly cut. After about one week (5-10 days) of cutting, spray the prepared solution of composition B on the leaves of the cuttings (Comparative Examples 1-4 do not have the solution of composition B, and use clean water instead).

[0139] (2) Statistics and analysis methods of cutting data

[0140] When the cuttings were cut to 60 days and 120 days, the root matrix of the cuttings was washed away, the number of adventitious roots produced by the cuttings was counted, and the length of the adventitious roots of the cuttings was measured with a vernier caliper. The data were statistically analyzed using the following formula;

[0141]

[0142] 2. Experimental Results

[0143] The rooting conditions of the cuttings treated with different embodiments and comparative examples for 60 days are as follows Figure 1 As shown in Table 1, the rooting conditions of the cuttings treated with different embodiments and comparative examples for 120 days are as follows: Figure 2 As shown in Table 2, the comparison results of the tea tree growth in the field of the embodiment and the comparative example are as follows: Figure 3 , Figure 4 and Figure 5 As shown, the results show that:

[0144] (1) After 60 days of cutting, a small amount of callus tissue was formed at the base of the cuttings in the embodiment, and a large number of adventitious roots were produced. The average number of adventitious roots of the cuttings treated in Examples 1, 2, and 3 reached 13.3, 16.5, and 14.5, respectively, and the average root lengths were 2.9 cm, 3.0 cm, and 2.9 cm, respectively, and the rooting rates were 76%, 78%, and 77%, respectively. In contrast, the average number of adventitious roots of the cuttings treated in Comparative Examples 1-8 was only 1.3, 3.3, 3.6, 3.9, 4.3, 2.8, 4.2, and 4.8, and the average root lengths were 0.9 cm, 1.2 cm, 0.8 cm, 1.3 cm, 1.8 cm, 1.7 cm, 1.5 cm, and 1.6 cm, respectively, and the rooting rates were 22%, 30%, 31%, 35%, 34%, 33%, 39%, and 42%, respectively.

[0145] In addition, from Figure 1 It can be seen that a small amount of callus tissue is formed at the base incision of the cuttings treated in Examples 1, 2, and 3, and a large number of root primordia and adventitious roots are produced. The number and length of adventitious roots are significantly better than those in the control group, and the rooting rate and survival rate are greatly improved; while the control group only forms a small amount or very small amount of adventitious roots, and the root system is poorly developed. The time when the cuttings treated in the examples start to take root, the number and length of adventitious roots produced are significantly better than those in the control group, and the survival rate of the cuttings treated in the examples is also significantly higher than that in the control group.

[0146] (2) After 120 days of cutting, the average number of adventitious roots of the cuttings treated in Examples 1, 2, and 3 reached 18.0, 24.8, and 19.6, respectively, the average root length was 15.8 cm, 20.7 cm, and 17.3 cm, respectively, and the rooting rates were 85%, 91%, and 89%, respectively. In contrast, the average number of adventitious roots of the cuttings treated in Comparative Examples 1-8 was only 2.8, 3.3, 4.1, 6.3, 5.7, 4.5, 5.9, and 6.2, the average root length was 1.6 cm, 2.5 cm, 2.9 cm, 3.7 cm, 3.5 cm, 3.2 cm, 4.2 cm, and 3.9 cm, respectively, and the rooting rates were 30%, 36%, 36%, 41%, 38%, 37%, 41%, and 43%, respectively.

[0147] The cuttings of the examples show more obvious advantages, with good root development and vigorous growth of tea seedlings, especially Example 2, which performs best, with a rooting rate of 91%, good root development and vigorous growth of tea seedlings. Compared with the comparative example, the examples effectively reduce the formation of a large number of rootless callus tissues, significantly promote the growth and extension of adventitious roots, and through synergistic effects, improve the quality and growth rate of the root system, and ultimately improve the overall survival rate and root development quality of tea tree cuttings.

[0148] (3) In addition, although the base of the cuttings treated in Comparative Example 4 has been soaked in the highly effective solution of Composition A (whose formula is the same as that of Example 2), the rooting and root development of the cuttings are significantly affected due to the lack of application of Composition B in the later stage. Specifically, the experimental results of Comparative Example 4 at 60 days and 120 days after cutting show that although the cuttings treated in Comparative Example 4 have adventitious roots, the number and length of adventitious roots are greatly improved compared with Comparative Examples 1-3, but the number and length of these adventitious roots are significantly less than that of the Examples, the root system is poorly developed, and the growth of the tea seedlings is average. The lack of Composition B causes the callus of the cuttings of Comparative Example 4 to develop into a large number of rootless callus in the later stage of rooting, and the number and length of adventitious roots are both small, resulting in the number and quality of adventitious roots not being as sufficient as in the Examples, and the speed and intensity of root development are also limited. At the same time, due to the lack of regulation of the growth balance of the aboveground and underground parts and the support of nutrients, the tea seedlings of Comparative Example 4 have insufficient root nutrition supply in the later growth, and the overall growth is not as good as in the Examples. This shows that the application of composition B not only promotes early rooting, but also plays an irreplaceable role in the later root development and plant growth improvement.

[0149] (4) By comparing the rooting conditions of the embodiment and comparative examples 5 and 6, it can be concluded that eugenol and diosgenin play an important role in promoting the rooting of tea tree cuttings. In comparative examples 5 and 6, eugenol and diosgenin were removed from the solution of composition A, respectively, which had a significant effect on the rooting of the cuttings. Specifically, at 60 days after cutting, the rooting rates of comparative examples 5 and 6 were 34% and 33%, respectively, which were significantly lower than the rooting rates of embodiments 1, 2, and 3 (76%, 78%, and 77%, respectively). At 120 days after cutting, the rooting rates of comparative examples 5 and 6 were also significantly lower than those of the embodiment, at 38% and 37%, respectively, and the number and length of the root system were also less, indicating that the removal of eugenol and diosgenin led to a significant decrease in the rooting effect, and the rooting rate and root development of the cuttings were inhibited, further proving that they are indispensable in the cutting process.

[0150] (5) In Comparative Examples 7 and 8, after the furamidol in Composition B was replaced by chlormequat and uniconazole, the root development was restricted, especially in inhibiting non-rooting callus and promoting adventitious root formation. Compared with Examples 1, 2, and 3, although chlormequat and uniconazole both have the effect of inhibiting plant stem elongation, the effect of comprehensive regulation of root development of difficult-to-root tea trees is not as good as furamidol. At 60 days of cutting, the rooting rates of Comparative Examples 7 and 8 were 39% and 42%, respectively. Compared with the examples, the number of roots and the length of the root system were relatively small. At 120 days of cutting, the rooting rates of Comparative Examples 7 and 8 were 41% and 43%, respectively, which were much lower than the rooting rates of Examples 1, 2, and 3 (85%, 91%, and 89%, respectively), and the root system length was also significantly lower than that of the examples.

[0151] Eugenol and diosgenin play an indispensable role in promoting adventitious root formation, while the role of flurprimidol is more effective in regulating root development, which further illustrates the importance of the synergistic effect of the components in the present invention, especially the direct or indirect effect of these components on root development. In summary, the present invention provides an efficient and economical solution for the cutting propagation of tea trees through the synergistic effect of ascorbic acid, brassinolide, indolebutyric acid, eugenol, diosgenin, and flurprimidol, diethylaminoethanol caproate, aluminum sulfate, ammonium dihydrogen phosphate, and zinc sulfate, effectively solves the problems of low rooting rate and slow rooting in traditional cuttings, greatly shortens the seedling raising time, reduces the seedling raising cost, and lays a solid foundation for the large-scale cutting propagation of tea trees.

[0152] Table 1 Rooting of cuttings 60 days after cutting

[0153]

[0154] Table 2 Rooting of cuttings 120 days after cutting

[0155]

[0156] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A composite plant growth regulator for tea tree cuttings, characterized in that: The invention comprises a composition A solution and a composition B solution which are used separately; the composition A solution comprises components with the following mass concentrations: 5-25 mg / L ascorbic acid, 0.5-1.8 mg / L brassinolide, 80-250 mg / L indolebutyric acid, 3-12 mg / L eugenol and 0.6-2.5 mg / L diosgenin; the composition B solution comprises components with the following mass concentrations: 2-10 mg / L furaprinol, 10-18 mg / L diethylaminoethanol hexanoate, 40-80 mg / L aluminum sulfate, 10-50 mg / L diammonium phosphate and 0.5-2 mg / L zinc sulfate.

2. Use of the composite plant growth regulator as claimed in claim 1 in promoting rooting of tea tree cuttings.

3. Use of the composite plant growth regulator as claimed in claim 1 in the preparation of a product for promoting rooting of tea tree cuttings.

4. Use of the composite plant growth regulator as claimed in claim 1 in reducing callus formation in tea tree cuttings.

5. Use of the composite plant growth regulator as claimed in claim 1 in the preparation of a product for reducing callus formation in tea tree cuttings.

6. Use of the composite plant growth regulator as claimed in claim 1 in improving the survival rate of tea tree cuttings.

7. Use of the composite plant growth regulator as claimed in claim 1 in the preparation of a product for improving the survival rate of tea tree cuttings.

8. Use of the composite plant growth regulator as claimed in claim 1 in tea tree cuttings.

9. A method for tea tree cutting seedling cultivation, characterized in that: Tea tree cuttings are soaked in the solution of composition A described in claim 1 for 12 to 16 hours, and then cut. After 5 to 10 days of cutting, the solution of composition B described in claim 1 is applied to the leaves of the cuttings.

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