Loading device for matrix-assisted laser desorption ionization time-of-flight mass spectrometry

Abstract

The invention discloses a loading device for matrix-assisted laser desorption ionization time-of-flight mass spectrometry, and the invention relates to the technical field of matrix-assisted laser desorption ionization time-of-flight mass spectrometer. The device comprises a shell one and a shell two which are arranged relatively to each other on the left and right sides, and by arranging a cleaning mechanism, a purge component in a cleaning component can be used to purge dust and particulate impurities on the surface of a sample target plate, so that the dust and particulate impurities float with the airflow, and at the same time, a dust suction component is used to suck the disturbed dust into the interior thereof for centralized storage, so as to achieve the purpose of removing dust and particulate impurities on the surface of the sample target plate; secondly, the method of using airflow to purge dust on the surface of the sample target plate can avoid direct contact with a sample to be detected on its surface, so as to ensure the integrity of the detected sample on the basis of cleaning dust and impurities, improve the cleaning effect on the sample target plate, and reduce the interference in the detection process, which is conducive to improving the accuracy of the detection result.

Description

Technical Field

[0001] The invention relates to the technical field of matrix-assisted laser desorption / ionization time-of-flight mass spectrometers, in particular to a loading device for matrix-assisted laser desorption / ionization time-of-flight mass spectrometers. Background Art

[0002] The loading device of matrix-assisted laser desorption / ionization time-of-flight mass spectrometry is a key component for loading samples into the mass spectrometer for analysis;

[0003] The currently available loading device includes: a shell, which forms a accommodating chamber for protecting the internal structure and maintaining certain environmental conditions; a target carrier, which is located in the accommodating chamber and is used to support and fix the sample target plate to be analyzed; and an injection device, which includes an injection channel for the target carrier body to enter and exit, and is used to send the target carrier into or move out of the analysis area of ​​the mass spectrometer.

[0004] The loading device for matrix-assisted laser desorption ionization time-of-flight mass spectrometry disclosed in the patent application with reference publication number CN108375626B drives the baffle on the target carrier to move by utilizing the sliding device for the target carrier to enter and exit the injection channel, and the baffle is abutted against the sealing member, thereby realizing that the loading device accommodating chamber always maintains vacuum isolation and sealing with the external environment during the process of loading and removing the biological target plate. In addition, the loading device has a simple structure and a small exhaust volume, and can effectively realize the isolation and sealing of the high vacuum environment and the low vacuum environment, or the high vacuum environment and the atmospheric environment in the loading device.

[0005] A comprehensive analysis of the above patents reveals the following defects:

[0006] During the use of the sample target plate in the loading device, dust and other granular stains will adhere to its surface due to material reaction, gas residue, and physical effects during the sputtering process; dust stains will become an error source and participate in the detection process together with the target, thereby affecting the stability of the detection process and the sputtering effect of the sample target plate, resulting in false positives and inaccurate detection results; secondly, hard and sharp particles will scratch the measuring head and sensor of the detection equipment, thereby affecting the sensitivity of the sensor and measuring head of the detection equipment and reducing the service life of the equipment.

[0007] Therefore, the present invention proposes a loading device for matrix-assisted laser desorption / ionization time-of-flight mass spectrometry to solve the above problems. Summary of the invention

[0008] In view of the deficiencies in the prior art, the present invention provides a loading device for matrix-assisted laser desorption ionization time-of-flight mass spectrometry, which solves the problem that during use, a sample target plate in the loading device will have granular stains such as dust attached to its surface due to material reaction, gas residue, and physical effects during the sputtering process. The dust stains will become an error source and participate in the detection process together with the target object, thereby affecting the stability of the detection process and the sputtering effect of the sample target plate, resulting in false positives and inaccurate detection results; hard and sharp particles will scratch the measuring head and sensor of the detection equipment, thereby affecting the sensitivity of the sensor and the measuring head of the detection equipment and reducing the service life of the equipment.

[0009] To achieve the above objectives, the present invention is implemented through the following technical solutions: a loading device for matrix-assisted laser desorption ionization time-of-flight mass spectrometry, comprising a shell one and a shell two arranged opposite to each other on the left and right, a partition plate is arranged between the shell one and the shell two, a displacement component for driving the sample target plate to move is arranged inside the shell one, the displacement component comprises a bearing plate fixedly arranged on the inner wall of the shell one and a linear module arranged on the top of the bearing plate, and a slide seat is slidably arranged on the top of the linear module;

[0010] A blocking slider is fixedly arranged on the top of the slide seat, a target platform is fixedly arranged on one end of the blocking slider close to the partition, a cleaning mechanism for cleaning the sample target plate is arranged on the side wall of the partition away from the target platform, and a sealing sleeve is fixedly arranged on the side wall opposite to the partition and the cleaning mechanism, a through groove matched with the blocking slider structure is formed on the side wall of the sealing sleeve, a sample injection device is arranged at one end of the cleaning mechanism, a groove is formed on the side wall of the target platform, and a rack for driving the cleaning mechanism to complete the cleaning of the sample target plate is fixedly arranged inside the groove.

[0011] Furthermore, the cleaning mechanism includes a cleaning rack fixedly arranged on the side wall of the partition and a through hole opened on the side wall of the cleaning rack, the top of the cleaning rack is provided with a mounting groove 1 connected to the through hole, the interior of the mounting groove 1 is detachably provided with a cleaning component for disturbing the dust on the surface of the sample target plate and collecting the raised dust through bolts, and the side wall of the cleaning rack is provided with a mounting groove 2 connected to the interior of the through hole, the interior of the mounting groove 2 is detachably provided with a driving component for driving the cleaning component to complete the disturbance and collection of the dust on the surface of the sample target plate through bolts.

[0012] Furthermore, the cleaning component includes a cleaning box that is detachably arranged inside an installation groove and a baffle that is detachably arranged on both sides of the outer wall of the cleaning box, a dust suction component and a purge component are respectively arranged on both sides of the interior of the cleaning box, one end of the dust suction component and the purge component penetrates the cleaning box and extends to the outside, and a transmission component for transmitting power to the dust suction component and the purge component is respectively arranged on both sides of the side walls of the cleaning box.

[0013] Furthermore, the dust suction assembly includes a dust collecting box fixedly arranged on the top of the cleaning box cavity and an elongated dust inlet opened at the bottom of the dust collecting box, filter plates are fixedly arranged on both sides of the interior of the dust collecting box, a transmission 1 is fixedly arranged on the side walls of the cleaning box and at one end of the dust collecting box, guide plates are removably arranged on both sides of the inner wall of the elongated dust inlet, and the two guide plates are arranged in an inverted "eight" shape, and a transmission shaft 1 is rotated inside the two filter plates and passes through them, one end of the transmission shaft 1 rotates through the dust collecting box and is fixedly connected to the output shaft of the transmission 1, a worm gear is fixedly arranged on the input shaft of the transmission 1, and a fan blade 1 is fixedly sleeved on the outer wall of the transmission shaft 1 and located between the filter plate and the inner wall of the dust collecting box.

[0014] Furthermore, the purge assembly includes a bellows fixedly arranged on the top of the cleaning box cavity and a plurality of air outlets evenly arranged on the bottom of the bellows, a transmission 2 is fixedly arranged on the side wall of the cleaning box and at one end of the bellows, a gear 1 is fixedly arranged on the input shaft of the transmission 2, a transmission shaft 2 is rotatably arranged inside the bellows, fan blades 2 are fixedly arranged on both sides of the outer wall of the transmission shaft 2, and one end of the transmission shaft 2 rotates through the bellows and is connected to the input shaft of the transmission 2.

[0015] Furthermore, the structure of the transmission component one is exactly the same as that of the transmission component two, and the transmission component one includes a transmission box one fixedly arranged on the side wall of the cleaning box, a worm is rotatably arranged inside the transmission box one, the bottom end of the worm rotates through the transmission box one and is fixedly provided with a worm meshing with the worm wheel, the bottom end of the worm rotates through the transmission box one and is fixedly provided with a gear two, and a plurality of exhaust ports one are evenly arranged on the outer wall of the transmission box one.

[0016] Furthermore, the driving assembly includes a transmission box 2 that is sealingly and slidably arranged inside the installation groove 2 and a protective plate that is detachably arranged on the side wall of the transmission box 2. A power transmission unit is arranged on both sides of the interior of the transmission box 2, and the two power transmission units are connected by a transmission belt.

[0017] Furthermore, the power transmission unit includes a transmission shaft three rotatably arranged on the inner wall of the transmission box two, the top end of the transmission shaft three rotates through the transmission box two and is fixedly provided with a gear three, and a gear four and a pulley are fixedly provided on the outer wall of the transmission shaft three and located inside the transmission box two.

[0018] Furthermore, the shell body 2 includes a shell body and an intake and exhaust pipe fixedly arranged on the side wall of the shell body and connected to the interior thereof. An operation port is opened on the top of the shell body, and a sealing plate is slidably and sealably arranged inside the operation port.

[0019] Furthermore, a sampling channel for providing space for placing the sample target plate is provided on the top of the sampling device.

[0020] The present invention provides a loading device for matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Compared with the prior art, it has the following beneficial effects:

[0021] 1. A loading device for matrix-assisted laser desorption ionization time-of-flight mass spectrometry can, by setting a cleaning mechanism, use a purge component in the cleaning component to purge dust and particulate impurities on the surface of a sample target plate, so that they float with the airflow, and at the same time use a dust suction component to suck the disturbed dust into the interior for centralized storage, thereby achieving the purpose of removing dust and particulate impurities from the surface of the sample target plate; secondly, the method of using airflow to purge dust on the surface of the sample target plate can avoid direct contact with the sample to be detected on its surface, thereby ensuring the integrity of the test sample while ensuring the cleaning of dust and impurities, thereby improving the cleaning effect of the sample target plate, and reducing interferences in the detection process, which is conducive to improving the accuracy of the detection result.

[0022] 2. The loading device for matrix-assisted laser desorption / ionization time-of-flight mass spectrometry forms a linkage through a driving component, a target stage, a dust suction component and a purge component. While the target stage is moving, the rack can be used to drive the gear four to rotate, thereby achieving the purpose of driving the worm in the transmission component one and the transmission component two to rotate, and then providing driving force for the dust suction component and the purge component, achieving the effect of synchronous operation of the dust suction component and the purge component, achieving the purpose of the dust being blown up by the purge component while the dust suction component immediately absorbs and collects the raised dust; secondly, there is no need to set up a separate power device for the dust suction component and the purge component to realize the operation of the two, which saves the cost of setting up the power device, and at the same time makes the structure simpler and is easy to use with subsequent inspection and maintenance work.

[0023] 3. A loading device for matrix-assisted laser desorption ionization time-of-flight mass spectrometry can be provided with a blocking slider, so that while loading a sample target plate, the blocking slider can be used to seal the through groove in the sealing sleeve, so that the internal spaces of the shell one and the shell two are isolated from each other, and the internal space of the shell one is always in a vacuum state; and after the sample target plate is loaded, the blocking slider re-enters the shell one, and the connection between the shell one and the shell two can be achieved; secondly, the contact area between the blocking slider and the inner wall of the sealing sleeve is relatively large, and a large contact area can be formed between the blocking slider and the inner wall of the sealing sleeve, so the sealing effect is better, even if a gap appears between a certain position on the surface of the blocking slider and the inner wall of the sealing sleeve, there is still a large area of ​​blocking range, thereby ensuring that the sealing between the blocking slider and the sealing sleeve is maintained intact. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1It is a schematic diagram of the overall three-dimensional structure of the present invention;

[0025] Figure 2 This is a schematic diagram of the second structure of the housing of the present invention;

[0026] Figure 3 This is a structural schematic diagram of the present invention without the housing 1 and the housing 2;

[0027] Figure 4 For the present invention Figure 3 A schematic diagram of the enlarged structure of part A;

[0028] Figure 5 This is a schematic diagram of the structure of the present invention in a disassembled state after the first shell and the second shell are removed;

[0029] Figure 6 This is a schematic diagram of the cleaning mechanism of the present invention in a disassembled state;

[0030] Figure 7 It is a schematic cross-sectional view of the cleaning mechanism of the present invention;

[0031] Figure 8 For the present invention Figure 7 A schematic diagram of the enlarged structure of part B in FIG.

[0032] Fig. 9 This is a schematic diagram of the structure of the cleaning component of the present invention in a disassembled state;

[0033] Fig.10 This is a schematic diagram of the first cross-sectional structure of the dust collection assembly of the present invention;

[0034] Fig.11 It is a second cross-sectional structural schematic diagram of the dust collection assembly of the present invention;

[0035] Fig.12 It is a schematic diagram of the cross-sectional structure of the purge assembly of the present invention;

[0036] Fig.13 It is a structural schematic diagram of a transmission component of the present invention;

[0037] Fig.14 It is a schematic diagram of the structure of the drive assembly in the exploded state of the present invention.

[0038] In the figure: 1, shell 1; 2, shell 2; 21, shell body; 22, air inlet and outlet pipes; 23, operation port; 24, sealing plate; 3, partition; 4, bearing plate; 5, linear module; 6, slide seat; 7, blocking slide block; 8, target platform; 9, cleaning mechanism; 91, cleaning rack; 92, through hole; 93, mounting slot 1; 94, cleaning assembly; 941, cleaning box; 942, baffle; 943, dust suction assembly; 9431, dust box; 9432, filter plate; 9433, transmission 1; 9434, guide plate; 9435, transmission shaft 1; 9436, fan blade 1; 9437, worm gear; 944, purge assembly; 9 441. bellows; 9442. air outlet; 9443. transmission two; 9444. gear one; 9445. transmission shaft two; 9446. fan blade two; 945. transmission component one; 9451. transmission box one; 9452. worm; 9453. gear two; 9454. exhaust port one; 946. transmission component two; 95. exhaust port two; 96. mounting slot two; 97. drive component; 971. transmission box two; 972. protective plate; 973. transmission shaft three; 974. gear three; 975. gear four; 976. pulley; 977. transmission belt; 10. sealing sleeve; 11. injection device; 12. rack. DETAILED DESCRIPTION

[0039] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] like Figures 1 to 14 The present invention provides three technical solutions for a loading device for matrix-assisted laser desorption ionization time-of-flight mass spectrometry, specifically including the following embodiments:

[0041] Embodiment 1: A loading device for matrix-assisted laser desorption ionization time-of-flight mass spectrometry, comprising a shell 1 and a shell 2 arranged opposite to each other on the left and right, a partition 3 is arranged between the shell 1 and the shell 2, a displacement assembly for driving the sample target plate to move is arranged inside the shell 1, the displacement assembly comprises a bearing plate 4 fixedly arranged on the inner wall of the shell 1 and a linear module 5 arranged on the top of the bearing plate 4, and a slide seat 6 is slidably arranged on the top of the linear module 5;

[0042] A blocking slider 7 is fixedly arranged on the top of the slide 6, and a target platform 8 is fixedly arranged on one end of the blocking slider 7 close to the partition 3, and a cleaning mechanism 9 for cleaning the sample target plate is arranged on the side wall of the partition 3 away from the target platform 8, and a sealing sleeve 10 is fixedly arranged on the side wall opposite to the partition 3 and the cleaning mechanism 9, and a through groove compatible with the structure of the blocking slider 7 is opened on the side wall of the sealing sleeve 10, a sample injection device 11 is arranged at one end of the cleaning mechanism 9, and a groove is opened on the side wall of the target platform 8, and a rack 12 for driving the cleaning mechanism 9 to complete the cleaning of the sample target plate is fixedly arranged inside the groove. A sealing rubber sleeve is fixedly sleeved on the outer wall of the blocking slider 7. When the blocking slider 7 slides in the through groove inside the sealing sleeve 10, the sealing rubber sleeve is used to ensure that the blocking slider 7 and the through groove are always in a sealed sliding connection state; an auxiliary through groove matching the through groove structure is opened on the side wall of the partition 3; after the target carrier 8 enters the injection channel of the injection device 11, the end of the blocking slider 7 close to the slide 6 just reaches the edge of the through groove inside the sealing sleeve 10, and when the target carrier 8 returns to enter the cleaning mechanism 9, the blocking slider 7 is still partially inside the sealing sleeve 10. The slide 6 and the linear module 5 are threadedly connected. The linear module 5 is used to drive the slide 6 to move a set distance to the left or right along its top. The linear module 5 is a known technology, and its internal structure and working principle are not described here.

[0043] The shell 2 2 includes a shell body 21 and an inlet and outlet pipe 22 fixedly arranged on the side wall of the shell body 21 and connected to the inside thereof. An operation port 23 is provided on the top of the shell body 21, and a sealing plate 24 is provided inside the operation port 23 for sealing and sliding. A slide groove is provided on the side wall opposite to the operation port 23, and the sealing plate 24 is slidably arranged in the slide groove, and a sealing gasket is provided between the sealing plate 24 and the slide groove. The inlet and outlet pipe 22 is connected to an external vacuum pump through a pipeline. The partition 3 is fixedly arranged on the side wall of the shell body 21, and the shell body 21 is detachably arranged on the side wall of the shell 1 1 by bolts.

[0044] The top of the sample injection device 11 is provided with a sample injection channel for providing space for placing the sample target plate, and the sample injection channel is connected with the through groove and the auxiliary through groove.

[0045] Embodiment 2: The main difference between this embodiment and the first technical solution is that: the loading device for matrix-assisted laser desorption ionization time-of-flight mass spectrometry, the cleaning mechanism 9 includes a cleaning frame 91 fixedly arranged on the side wall of the partition 3 and a through hole 92 opened on the side wall of the cleaning frame 91, the top of the cleaning frame 91 is provided with a mounting groove 1 93 connected to the through hole 92, the interior of the mounting groove 1 93 is detachably provided with a cleaning component 94 for disturbing the dust on the surface of the sample target plate and collecting the raised dust through bolts, the side wall of the cleaning frame 91 is provided with a mounting groove 2 96 connected to the interior of the through hole 92, the interior of the mounting groove 2 96 is detachably provided with a driving component 97 for driving the cleaning component 94 to complete the disturbance and collection of the dust on the surface of the sample target plate through bolts. One side wall of the cleaning frame 91 is connected to the side wall of the partition 3 by bolts, and the other side wall is connected to the injection device 11, and the through hole 92 is connected to the through groove and the injection channel inside the injection device 11.

[0046] The cleaning assembly 94 includes a cleaning box 941 detachably arranged inside the installation slot 1 93 and baffles 942 detachably arranged on both sides of the outer wall of the cleaning box 941. A dust collecting assembly 943 and a purge assembly 944 are respectively arranged on both sides of the cleaning box 941. One end of the dust collecting assembly 943 and the purge assembly 944 penetrates the cleaning box 941 and extends to the outside. A transmission assembly 1 945 for transmitting power to the dust collecting assembly 943 and the purge assembly 944 is respectively arranged on both sides of the side wall of the cleaning box 941. A plurality of exhaust holes 3 are provided on the side wall of the cleaning box 941 near the transmission 1 9433 and the transmission 2 9443. The exhaust holes 3 are connected with the exhaust port 2 95 and the exhaust port 1 9454.

[0047] The dust collection assembly 943 includes a dust collecting box 9431 fixedly arranged on the top of the cleaning box 941 cavity and a long strip dust inlet opened at the bottom of the dust collecting box 9431, filter plates 9432 are fixedly arranged on both sides of the interior of the dust collecting box 9431, a transmission 9433 is fixedly arranged on the side wall of the cleaning box 941 and at one end of the dust collecting box 9431, and guide plates 9434 are detachably arranged on both sides of the inner wall of the long strip dust inlet, and the two guide plates 9434 are arranged in an inverted "eight" shape, and a transmission shaft 9435 is rotated and penetrated inside the two filter plates 9432, one end of the transmission shaft 9435 rotates and penetrates the dust collecting box 9431 and is fixedly connected to the output shaft of the transmission 9433, a worm gear 9437 is fixedly arranged on the input shaft of the transmission 9433, and a fan blade 9436 is fixedly sleeved on the outer wall of the transmission shaft 9435 and located between the filter plate 9432 and the inner wall of the dust collecting box 9431. Both ends of the dust box 9431 are provided with a plurality of exhaust holes 4 for connecting the dust box 9431 with the external environment. The exhaust hole 4 is connected to the exhaust hole 3. The two fan blades 1 9436 are symmetrically arranged relative to the center of the transmission shaft 1 9435, and are both used to draw external air into the interior of the dust box 9431. The guide plate 9434 is arranged between the opposite side walls of the two filter plates 9432. The transmission 1 9433 is used to increase the rotation speed of the transmission shaft 1 9435.

[0048] The purge assembly 944 includes a bellows 9441 fixedly arranged at the top of the cavity of the cleaning box 941 and a plurality of air outlets 9442 uniformly arranged at the bottom of the bellows 9441. A transmission 2 9443 is fixedly arranged on the side wall of the cleaning box 941 and located at one end of the bellows 9441. A gear 1 9444 is fixedly arranged on the input shaft of the transmission 2 9443. A transmission shaft 2 9445 is rotatably arranged inside the bellows 9441. Fan blades 2 9446 are fixedly arranged on both sides of the outer wall of the transmission shaft 2 9445. One end of the transmission shaft 2 9445 rotates through the bellows 9441 and is connected to the input shaft of the transmission 2 9443. Multiple exhaust holes 5 are arranged at both ends of the bellows 9441 to connect the bellows 9441 to the external environment. Exhaust hole 5 is connected to exhaust hole 3. The two fan blades 9446 are symmetrically arranged with respect to the center of the transmission shaft 9445, and are used to extract the air inside the bellows 9441 to the outside. The transmission 9443 is used to increase the rotation speed of the transmission shaft 9445.

[0049] The structure of transmission component 1 945 is exactly the same as that of transmission component 2 946. Transmission component 1 945 includes a transmission box 1 9451 fixedly arranged on the side wall of the cleaning box 941. A worm 9452 is rotatably arranged inside the transmission box 1 9451. The bottom end of the worm 9452 rotates through the transmission box 1 9451 and is fixedly provided with a worm 9452 meshing with the worm wheel 9437. The bottom end of the worm 9452 rotates through the transmission box 1 9451 and is fixedly provided with a gear 2 9453. A plurality of exhaust ports 1 9454 are evenly arranged on the outer wall of the transmission box 1 9451.

[0050] Embodiment 3: The main difference between this embodiment and the second technical solution is that: the loading device for matrix-assisted laser desorption ionization time-of-flight mass spectrometry, the driving assembly 97 includes a transmission box 971 sealed and slidably arranged inside the second mounting groove 96 and a protective plate 972 detachably arranged on the side wall of the transmission box 971, and a power transmission unit is arranged on both sides of the interior of the transmission box 971, and the two power transmission units are connected by a transmission belt 977.

[0051] The power transmission unit includes a transmission shaft 3 973 rotatably arranged on the inner wall of the transmission box 2 971, the top of the transmission shaft 3 973 rotatably penetrates the transmission box 2 971 and is fixedly provided with a gear 3 974, and the outer wall of the transmission shaft 3 973 and located inside the transmission box 2 971 are respectively fixedly provided with a gear 4 975 and a pulley 976. The transmission belt 977 is jointly sleeved on the outer wall of the pulleys 976 in the two power transmission units; both sides of the outer wall of the protective plate 972 are provided with avoidance grooves, and the gears 4 975 in the two power transmission units respectively penetrate the avoidance grooves at the corresponding positions and extend to the outside; and the gears 3 974 in the two power transmission units are respectively meshed and connected with the gears 2 9453 in the transmission assembly 1 945 and the transmission assembly 2 946; the rack 12 is meshed and connected with the gear 4 975 when it meets.

[0052] During use, when it is necessary to load the sample target plate, the linear module 5 pushes the slide 6 toward the sealing sleeve 10 according to the preset program. Since the through groove space in the sealing sleeve 10 is much larger than the outer contour of the target carrier 8, the target carrier 8 does not contact the inner wall of the through groove after entering the sealing sleeve 10, and enters the cleaning mechanism 9 through the partition 3. When the linear module 5 pushes the slide 6 to the specified position according to the preset program, the target carrier 8 has entered the injection channel of the injection device 11. At the same time, the blocking slider 7 enters the through groove in the sealing sleeve 10, and the left end of the blocking slider 7 is close to the left side wall of the sealing sleeve 10. Since a sealing rubber sleeve is provided on the surface of the blocking slider 7, after the blocking slider 7 enters the through groove in the sealing sleeve 10, the internal space of the housing 1 is sealed and still maintains a vacuum state.

[0053] Next, the valve connected to the inlet and outlet pipes 22 is opened to connect the interior of the housing 2 with the external environment. At this time, the sealing plate 24 is pushed to one side to expose the operating port 23, and then the sample target plate is placed in the target carrier 8. Next, the linear module 5 is started again to pull the slide 6 to the first position at a uniform speed. After reaching the first position, the linear module 5 stops working, and the first position is that the target carrier 8 enters the sealing sleeve 10, and the end of the blocking slide 7 close to the partition 3 just reaches the left end of the through groove inside the sealing sleeve 10, and is not separated from the through groove. state; during the time period when the blocking slider 7 moves, the rack 12 on the side wall of the target carrier 8 is meshed with the gear four 975 at two positions or with one of the gear four 975, and drives the transmission shaft three 973 to rotate. Since the pulleys 976 on the two transmission shafts three 973 transmit power through the transmission belt 977, at this time, the gear three 974 at the two positions are respectively meshed and connected with the gear two 9453 in the transmission assembly one 945 and the transmission assembly two 946 at the corresponding positions, so that the worm 9452 at the two positions can rotate;

[0054] Since the worm 9452 in the transmission component 1 945 and the transmission component 2 946 are respectively meshed and connected with the gear 1 9444 in the dust collection component 943 and the purge component 944 at the corresponding positions, in the purge component 944, the low speed input by the gear 1 9444 is accelerated by the transmission 2 9443 and drives the transmission shaft 2 9445 to rotate at a high speed, and the two fan blades 2 9446 allow the external air to enter the interior through the exhaust holes 5 at both ends of the bellows 9441, and blow it onto the surface of the sample target plate through the multiple air outlets 9442, and the dust attached to the surface of the sample target plate is blown up;

[0055] At the same time, in the dust collection assembly 943, the transmission 1 9433 accelerates the speed input by the worm gear 9437 and then outputs it, the transmission shaft 1 9435 drives the fan blade 1 9436 to rotate at a high speed, and the external air enters the dust box 9431 through the long strip dust inlet at the bottom of the dust box 9431, and the dust disturbed and flying on the surface of the sample target plate is sucked into the dust box 9431 along with the flowing air. Since the two guide plates 9434 are in an inverted "eight" shape, the dust is filtered by the filter plate 9432 and retained in the dust box 9431, and the air is discharged through the exhaust holes at both ends of the dust box 9431;

[0056] Next, after the slide 6 reaches the first position, the shell 2 2 is evacuated with the help of an external vacuum pump to maintain the same vacuum degree as the shell 1. At this time, the linear module 5 is started again to drive the target stage 8 to move to the inside of the shell 1, and the sample target plate in the target stage 8 reaches the detection position, and the sample on the surface of the sample target plate can be detected.

[0057] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0058] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A loading device for matrix-assisted laser desorption ionization time-of-flight mass spectrometry, comprising a first shell (1) and a second shell (2) arranged opposite to each other, a partition (3) being arranged between the first shell (1) and the second shell (2), characterized in that: A displacement assembly for driving the sample target plate to move is arranged inside the housing 1 (1), the displacement assembly comprising a bearing plate (4) fixedly arranged on the inner wall of the housing 1 (1) and a linear module (5) arranged on the top of the bearing plate (4), and a slide seat (6) is slidably arranged on the top of the linear module (5); A blocking slider (7) is fixedly arranged on the top of the slide seat (6); a target carrier (8) is fixedly arranged on one end of the blocking slider (7) close to the partition (3); a cleaning mechanism (9) for cleaning the sample target plate is arranged on the side wall of the partition (3) away from the target carrier (8); a sealing sleeve (10) is fixedly arranged on the side wall opposite to the cleaning mechanism (9); a through groove matching the structure of the blocking slider (7) is provided on the side wall of the sealing sleeve (10); a sample injection device (11) is provided on one end of the cleaning mechanism (9); a groove is provided on the side wall of the target carrier (8); a rack (12) for driving the cleaning mechanism (9) to clean the sample target plate is fixedly arranged inside the groove; The cleaning mechanism (9) comprises a cleaning frame (91) fixedly mounted on the side wall of the partition (3) and a through hole (92) formed on the side wall of the cleaning frame (91); a first mounting groove (93) connected to the through hole (92) is formed on the top of the cleaning frame (91); a cleaning component (94) for disturbing dust on the surface of the sample target plate and collecting the raised dust is detachably provided inside the first mounting groove (93) by means of bolts; a second mounting groove (96) connected to the inside of the through hole (92) is formed on the side wall of the cleaning frame (91); a driving component (97) for driving the cleaning component (94) to disturb and collect dust on the surface of the sample target plate is detachably provided inside the second mounting groove (96) by means of bolts; The cleaning assembly (94) comprises a cleaning box (941) detachably arranged inside the first installation slot (93) and baffles (942) detachably arranged on both sides of the outer wall of the cleaning box (941); a dust collection assembly (943) and a purge assembly (944) are respectively arranged on both sides of the inside of the cleaning box (941); one end of the dust collection assembly (943) and the purge assembly (944) penetrate the cleaning box (941) and extend to the outside; and a transmission assembly (945) for transmitting power to the dust collection assembly (943) and the purge assembly (944) is respectively arranged on both sides of the side wall of the cleaning box (941).

2. The loading device for matrix-assisted laser desorption / ionization time-of-flight mass spectrometry according to claim 1, characterized in that: The dust collection assembly (943) comprises a dust collection box (9431) fixedly arranged at the top of the cavity of the cleaning box (941) and a long strip dust inlet port opened at the bottom of the dust collection box (9431); filter plates (9432) are fixedly arranged on both sides of the interior of the dust collection box (9431); a transmission 1 (9433) is fixedly arranged on the side wall of the cleaning box (941) and at one end of the dust collection box (9431); guide plates (9434) are detachably arranged on both sides of the inner wall of the long strip dust inlet port; the two guide plates (9434) are fixedly arranged on the side wall of the cleaning box (941) and at one end of the dust collection box (9431); The transmission shaft 1 (9435) is arranged in an inverted "eight" shape, and the two filter plates (9432) rotate together and penetrate a transmission shaft 1 (9435). One end of the transmission shaft 1 (9435) rotates through a dust box (9431) and is fixedly connected to an output shaft of a transmission 1 (9433). A worm gear (9437) is fixedly arranged on the input shaft of the transmission 1 (9433). A fan blade 1 (9436) is fixedly sleeved on the outer wall of the transmission shaft 1 (9435) and located between the filter plates (9432) and the inner wall of the dust box (9431).

3. The loading device for matrix-assisted laser desorption / ionization time-of-flight mass spectrometry according to claim 1, characterized in that: The purge assembly (944) comprises a bellows (9441) fixedly arranged at the top of the cavity of the cleaning box (941) and a plurality of air outlets (9442) uniformly arranged at the bottom of the bellows (9441); a second transmission (9443) is fixedly arranged on the side wall of the cleaning box (941) and located at one end of the bellows (9441); a gear (9444) is fixedly arranged on the input shaft of the second transmission (9443); a second transmission shaft (9445) is rotatably arranged inside the bellows (9441); two blades (9446) are fixedly sleeved on both sides of the outer wall of the second transmission shaft (9445); and one end of the second transmission shaft (9445) rotatably passes through the bellows (9441) and is connected to the input shaft of the second transmission (9443).

4. The loading device for matrix-assisted laser desorption / ionization time-of-flight mass spectrometry according to claim 2, characterized in that: The structure of the transmission component 1 (945) is completely the same as that of the transmission component 2 (946). The transmission component 1 (945) comprises a transmission box 1 (9451) fixedly arranged on the side wall of the cleaning box (941), a worm (9452) being rotatably arranged inside the transmission box 1 (9451), a worm (9452) being rotatably arranged at the bottom end of the worm (9452) passing through the transmission box 1 (9451) and being fixedly arranged with a worm (9452) meshingly connected with a worm wheel (9437), a gear 2 (9453) being fixedly arranged at the bottom end of the worm (9452) passing through the transmission box 1 (9451) and being fixedly arranged with a gear 2 (9453), and a plurality of exhaust ports 1 (9454) being evenly arranged on the outer wall of the transmission box 1 (9451).

5. The loading device for matrix-assisted laser desorption / ionization time-of-flight mass spectrometry according to claim 1, characterized in that: The driving assembly (97) comprises a second transmission box (971) sealingly and slidably disposed inside the second installation groove (96) and a protective plate (972) detachably disposed on the side wall of the second transmission box (971), wherein a power transmission unit is disposed on both sides of the interior of the second transmission box (971), and the two power transmission units are connected via a transmission belt (977).

6. The loading device for matrix-assisted laser desorption / ionization time-of-flight mass spectrometry according to claim 5, characterized in that: The power transmission unit comprises a transmission shaft three (973) rotatably arranged on the inner wall of the transmission box two (971), the top end of the transmission shaft three (973) rotatably passes through the transmission box two (971) and is fixedly provided with a gear three (974), and a gear four (975) and a pulley (976) are fixedly provided on the outer wall of the transmission shaft three (973) and located inside the transmission box two (971).

7. The loading device for matrix-assisted laser desorption / ionization time-of-flight mass spectrometry according to claim 1, characterized in that: The second shell (2) comprises a shell body (21) and an inlet and outlet pipe (22) fixedly arranged on a side wall of the shell body (21) and connected to the interior thereof; an operating port (23) is provided at the top of the shell body (21); a sealing plate (24) is provided inside the operating port (23) in a sealing and sliding manner.

8. The loading device for matrix-assisted laser desorption / ionization time-of-flight mass spectrometry according to claim 1, characterized in that: The top of the sample injection device (11) is provided with a sample injection channel for providing space for placing a sample target plate.

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