Cartilage repair hydrogel preparation management system

Abstract

The invention discloses a cartilage repair hydrogel preparation management system in the field of biomedical materials and tumor drug therapy, the cartilage repair hydrogel preparation management system comprises a preparation module and a simulation module, the preparation module is used for preparing porous structure hydrogel with the porosity of 80%-90%; the simulation module is used for constructing an integrated three-dimensional cartilage repair simulation system and comprises a three-dimensional unit, a structural unit, a lower bone unit, a mechanical unit, a self-healing unit, a lubricating unit, a conductive unit and an antibacterial unit; the device is scientific and reasonable, a three-dimensional cartilage repair simulation system in the simulation module is used for carrying out simulation detection on the reaction process and performance of the porous structure hydrogel, the simulation module feeds back a simulation result to the preparation module, and the preparation module adjusts the preparation of the porous structure hydrogel according to the simulation result. The problem that a traditional system cannot feed back and adjust porous structure hydrogel preparation parameters is solved.

Description

Technical Field

[0001] The invention belongs to the field of biomedical materials and tumor drug treatment, and specifically is a cartilage repair hydrogel preparation and management system. Background Art

[0002] Cartilage repair hydrogel is a biomaterial designed to mimic the structure and function of cartilage to promote the repair of damaged cartilage. In recent years, scientists have made a series of advances in the research of cartilage repair hydrogel, developed a variety of new hydrogel materials, and explored their applications in cartilage regeneration.

[0003] The cartilage repair hydrogel preparation system faces many challenges in design and application. The biocompatibility and biodegradability of the hydrogel are key considerations, and it is necessary to ensure that the hydrogel can promote cell growth and differentiation in the body. The prepared hydrogel needs to have appropriate porosity, structural size, and connectivity of the microporous structure to meet the repair needs of different damaged parts. In the cartilage repair hydrogel preparation system, the traditional simulation system mainly focuses on how to simulate the composition and structure of natural cartilage in order to develop hydrogel materials that can effectively promote cartilage repair and regeneration. However, it should be noted that the cartilage repair process and various performance parameters are not simulated.

[0004] To sum up, how to add a three-dimensional cartilage repair simulation system to the cartilage repair hydrogel preparation system and perform three-dimensional simulation feedback through the three-dimensional cartilage repair simulation system to adjust the hydrogel preparation parameters has become a problem that technical personnel in this field urgently need to solve. Therefore, it is necessary to propose a cartilage repair hydrogel preparation management system. Summary of the invention

[0005] In order to solve the above problems, the purpose of the present invention is to provide a cartilage repair hydrogel preparation and management system, which simulates and detects the reaction process and performance of the porous structure hydrogel through the three-dimensional cartilage repair simulation system in the simulation module. The simulation module then feeds back the simulation results to the preparation module, and the preparation module adjusts the preparation of the porous structure hydrogel according to the simulation results.

[0006] In order to achieve the above-mentioned object, the technical scheme of the present invention is as follows: a cartilage repair hydrogel preparation and management system, comprising a preparation module and a simulation module, the preparation module is used to prepare a porous structure hydrogel with a porosity of 80%-90%;

[0007] The simulation module is used to construct an integrated three-dimensional cartilage repair simulation system, and the simulation module includes a three-dimensional unit, a structural unit, a lower bone unit, a mechanical unit, a self-healing unit, a lubrication unit, a conductive unit and an antibacterial unit;

[0008] The three-dimensional unit is used to collect three-dimensional simulation information of the porous structure hydrogel, and the three-dimensional simulation information is used to assist the simulation module in building a three-dimensional cartilage repair simulation system. The three-dimensional unit simulates and monitors the simulation results of the internal reaction changes of the porous structure hydrogel through the three-dimensional cartilage repair simulation system. After the three-dimensional unit obtains the simulation results of the internal reaction changes, it feeds back the simulation results of the internal reaction changes to the preparation module;

[0009] The structural unit is used to simulate and monitor the internal structural change simulation results of the hydrogel during the preparation process through a three-dimensional cartilage repair simulation system. The internal structural change simulation results include the pore structure stability and the degree of cross-linking. After the structural unit obtains the internal structural change simulation results, it feeds back the internal structural change simulation results to the preparation module.

[0010] Furthermore, the method for preparing the porous structure hydrogel in the preparation module comprises the following steps:

[0011] Step 1, simulating the cross-linking structure of the porous hydrogel into the three-dimensional structure of the natural cartilage extracellular matrix; the cross-linking structure is prepared by an enzyme cross-linking method;

[0012] Step 2, using materials with self-healing ability to prepare porous structure hydrogel, the materials with self-healing ability include gelatin and chitosan;

[0013] Step 3, adding growth factors into the porous structure hydrogel; the growth factors include TGF-β3 and BMP-2;

[0014] Step 4, adding bone regeneration factors into the porous structure hydrogel; the bone regeneration factors include BMP and vitamin D3;

[0015] Step 5, adding a lubricant to the porous structure hydrogel; the lubricant includes hyaluronic acid and glycerol;

[0016] Step six, adding a conductive material to the porous structure hydrogel; the conductive material includes carbon nanotubes and graphene;

[0017] Step seven, adding antimicrobial agents into the porous structure hydrogel; the antimicrobial agents include silver nanoparticles and antimicrobial peptides.

[0018] Furthermore, the 3D simulation information in the 3D cell includes porous structure characteristics, material properties, cell behavior simulation, reaction dynamics, temperature, pH value, and oxygen concentration.

[0019] Furthermore, after the structural unit feeds back the structural change simulation results to the preparation module, the preparation module adjusts the cross-linking agent concentration and cross-linking time in the enzyme cross-linking method. The compressive strength of the porous structure hydrogel prepared by the enzyme cross-linking method is 0.5-1.5MPa.

[0020] Furthermore, the lower bone unit is used to simulate the lower bone regeneration simulation results of cartilage by porous structure hydrogel through a three-dimensional cartilage repair simulation system. After the lower bone unit obtains the lower bone regeneration simulation results, the lower bone unit feeds back the lower bone regeneration simulation results to the preparation module. The preparation module adjusts the concentration and reaction time of TGF-β3, BMP-2, BMP and vitamin D3. The GAG ​​content of the lower bone regeneration tissue is 20-30 mg / g.

[0021] Furthermore, the mechanical unit is used to simulate the mechanical properties of the porous structure hydrogel through a three-dimensional cartilage repair simulation system. After obtaining the mechanical properties simulation results, the mechanical unit feeds back the mechanical properties simulation results to the preparation module. The preparation module adjusts the concentration and reaction time of TGF-β3, BMP-2, BMP and vitamin D3 according to the mechanical properties simulation results. The mineralization degree of the bone regeneration tissue is 50%-70%.

[0022] Furthermore, the self-healing unit is used to simulate the self-healing ability simulation results of the porous structure hydrogel through a three-dimensional cartilage repair simulation system. After the self-healing unit obtains the self-healing ability simulation results, it feeds back the self-healing ability simulation results to the preparation module. The preparation module adjusts the overall proportion and reaction time of gelatin and chitosan according to the self-healing ability simulation results. The self-healing efficiency of the porous structure hydrogel is 80%-90%.

[0023] Furthermore, the lubrication unit is used to simulate the lubrication performance simulation results of the porous structure hydrogel through a three-dimensional cartilage repair simulation system. After the lubrication unit obtains the lubrication performance simulation results, it feeds back the lubrication performance simulation results to the preparation module. The preparation module adjusts the concentration of hyaluronic acid and glycerol and the reaction time according to the lubrication performance simulation results. The friction coefficient of the porous structure hydrogel is 0.01-0.05.

[0024] Furthermore, the conductive unit is used to simulate the conductive performance simulation results of the hydrogel through a three-dimensional cartilage repair simulation system. After obtaining the conductive performance simulation results, the conductive unit feeds back the conductive performance simulation results to the preparation module. The preparation module adjusts the proportion and concentration of carbon nanotubes and graphene according to the conductive performance simulation results. The conductivity of the porous structure hydrogel is 1S / cm-3S / cm.

[0025] Furthermore, the antibacterial unit is used to simulate the antibacterial performance of the hydrogel through a three-dimensional cartilage repair simulation system. After obtaining the antibacterial performance simulation results, the antibacterial performance simulation results are fed back to the preparation module. The preparation module adjusts the concentration and reaction time of the silver nanoparticles and antibacterial peptides according to the antibacterial performance simulation results. The porous structure hydrogel prevents bacterial infection by 95%-99%.

[0026] The principles and beneficial effects of the basic solution are:

[0027] 1. The system integrates the preparation module and the simulation module through an integrated three-dimensional cartilage repair simulation system. The simulation module is used to simulate and monitor the reaction process and performance of the hydrogel, while the preparation module adjusts the preparation parameters according to the simulation results, thus improving the integration of the system.

[0028] 2. Multiple units within the system, including three-dimensional units, structural units, subbone units, mechanical units, self-healing units, lubrication units, conductive units and antibacterial units, each perform their respective functions. By simulating and monitoring different aspects of performance, the repair effect of the hydrogel is comprehensively evaluated, and corresponding adjustment suggestions are given, thereby improving the feedback efficiency of the system.

[0029] 3. The traditional hydrogel development process requires multiple experiments and tests, which is costly. However, the system of the present invention reduces the number of experiments and reduces the development cost by simulating the cartilage repair process of porous hydrogel.

[0030] 4. Through simulation and optimization, the prepared porous structure hydrogel achieves the optimal configuration in terms of porosity, cross-linking structure, growth factors, lubricants, conductive materials and antibacterial agents, thereby improving the effect of cartilage repair.

[0031] 5. The integration of the system and the synergy of multiple units make the entire preparation process more intelligent and automated, reduce the interference of human factors, and improve the accuracy and reliability of preparation.

[0032] 6. The structure of the extracellular matrix of natural cartilage can be simulated to provide a microenvironment similar to that of natural cartilage, which is conducive to cell proliferation and differentiation. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 The figure is a flow chart of a cartilage repair hydrogel preparation and management system according to an embodiment of the present invention. DETAILED DESCRIPTION

[0034] The following is further described in detail through specific implementation methods:

[0035] The embodiment is basically as shown in the attached Figure 1 Shown: A cartilage repair hydrogel preparation and management system, including a preparation module and a simulation module, wherein the preparation module is used to prepare a porous structure hydrogel with a porosity controlled at 90%;

[0036] The method for preparing the porous structure hydrogel in the preparation module comprises the following steps:

[0037] Step 1: The cross-linked structure of the porous hydrogel simulates the three-dimensional structure of the natural cartilage extracellular matrix, simulates the structure of the natural cartilage extracellular matrix, provides a microenvironment similar to that of natural cartilage, and is conducive to cell proliferation and differentiation. The cross-linked structure is prepared by an enzyme cross-linking method.

[0038] Step 2: Use materials with self-healing ability to prepare porous structure hydrogels, which can improve the network cross-linking and self-recovery ability of the implanted scaffold in the body and help improve the efficiency of cartilage repair. Materials with self-healing ability include gelatin and chitosan.

[0039] Step three, adding growth factors to the porous structure hydrogel to promote cartilage regeneration, the growth factors include TGF-β3 and BMP-2.

[0040] Step 4: Add factors that promote bone regeneration to the porous structure hydrogel to promote the synchronous regeneration of subchondral bone, which helps to restore the overall function of the joint. Factors that promote bone regeneration include BMP and vitamin D3.

[0041] Step five: adding lubricants to the porous structure hydrogel. The lubricants can reduce friction and protect the joint surface. The lubricants include hyaluronic acid and glycerol.

[0042] Step six: Add conductive materials to the porous structure hydrogel. The conductive materials can promote the transmission of electrical signals between cells. The conductive materials include carbon nanotubes and graphene.

[0043] Step seven, adding antibacterial agents to the porous structure hydrogel, the antibacterial agents can prevent bacterial infection, the antibacterial agents include silver nanoparticles and antibacterial peptides.

[0044] The simulation module is used to construct an integrated three-dimensional cartilage repair simulation system, and the simulation module includes a three-dimensional unit, a structural unit, a lower bone unit, a mechanical unit, a self-healing unit, a lubrication unit, a conductive unit and an antibacterial unit.

[0045] The three-dimensional unit is used to collect three-dimensional simulation information of porous structure hydrogel. The three-dimensional simulation information assists the simulation module to build a three-dimensional cartilage repair simulation system. The three-dimensional unit simulates and monitors the simulation results of internal reaction changes of porous structure hydrogel through the three-dimensional cartilage repair simulation system. After the three-dimensional unit obtains the simulation results of internal reaction changes, it feeds back the simulation results of internal reaction changes to the preparation module; the three-dimensional simulation information in the three-dimensional unit includes porous structure characteristics, material properties, cell behavior simulation, reaction dynamic changes, temperature, pH value and oxygen concentration.

[0046] The structural unit is used to simulate and monitor the internal structural changes of the hydrogel during the preparation process through a three-dimensional cartilage repair simulation system. The internal structural change simulation results include the pore structure stability and the degree of cross-linking. After the structural unit obtains the internal structural change simulation results, it feeds back the internal structural change simulation results to the preparation module. The preparation module adjusts the cross-linking agent concentration and cross-linking time in the enzyme cross-linking method to make the compressive strength of the porous structure hydrogel reach 1.5MPa, ensuring that the hydrogel has a microstructure and mechanical properties similar to natural cartilage.

[0047] The lower bone unit is used to simulate the lower bone regeneration simulation results of cartilage by porous structure hydrogel through a three-dimensional cartilage repair simulation system. After the lower bone unit obtains the lower bone regeneration simulation results, the lower bone unit feeds back the lower bone regeneration simulation results to the preparation module. The preparation module adjusts the concentration and reaction time of TGF-β3, BMP-2, BMP and vitamin D3 to make the GAG ​​content of the lower bone regeneration tissue at 30 mg / g. A higher GAG content helps promote the regeneration and repair of bone tissue, especially after fracture or bone injury, GAG can accelerate the proliferation and differentiation of bone cells and promote the healing of bone tissue.

[0048] The mechanical unit is used to simulate the mechanical properties of porous structure hydrogels through a three-dimensional cartilage repair simulation system. After obtaining the mechanical properties simulation results, the mechanical unit feeds back the mechanical properties simulation results to the preparation module. The preparation module adjusts the concentration and reaction time of TGF-β3, BMP-2, BMP and vitamin D3 according to the mechanical properties simulation results, so that the mineralization degree of the lower bone regeneration tissue is 70%. The increase in the degree of mineralization means that the deposition of minerals calcium and phosphorus in the bones increases, which helps to enhance the hardness and load-bearing capacity of the bones and provide solid support for the body.

[0049] The self-healing unit is used to simulate the self-healing ability of the porous structure hydrogel through a three-dimensional cartilage repair simulation system. After obtaining the self-healing ability simulation results, the self-healing ability simulation results are fed back to the preparation module. The preparation module adjusts the overall proportion and reaction time of gelatin and chitosan according to the self-healing ability simulation results to make the self-healing efficiency of the porous structure hydrogel 90%. The high self-healing efficiency means that the hydrogel can quickly restore its structure and function after being damaged, thereby reducing the inconvenience and cost caused by frequent material replacement.

[0050] The lubrication unit is used to simulate the lubrication performance simulation results of the porous structure hydrogel through a three-dimensional cartilage repair simulation system. After the lubrication unit obtains the lubrication performance simulation results, it feeds back the lubrication performance simulation results to the preparation module. The preparation module adjusts the concentration of hyaluronic acid and glycerol and the reaction time according to the lubrication performance simulation results to make the friction coefficient of the porous structure hydrogel at 0.01; the lubrication performance of the porous structure hydrogel with a friction coefficient of 0.01 is much better than that of general hydrogels, and can reduce the wear of cartilage.

[0051] The conductive unit is used to simulate the conductive performance of the hydrogel through a three-dimensional cartilage repair simulation system. After obtaining the conductive performance simulation results, the conductive unit feeds back the conductive performance simulation results to the preparation module. The preparation module adjusts the proportion and concentration of carbon nanotubes and graphene according to the conductive performance simulation results to make the conductivity of the porous structure hydrogel reach 3S / cm. Having conductivity means that it can promote the transmission of electrical signals between cells and accelerate cell proliferation and differentiation.

[0052] The antibacterial unit is used to simulate the antibacterial performance of the hydrogel through a three-dimensional cartilage repair simulation system. After obtaining the antibacterial performance simulation results, the antibacterial performance simulation results are fed back to the preparation module. The preparation module adjusts the concentration and reaction time of silver nanoparticles and antimicrobial peptides according to the antibacterial performance simulation results, so that the porous structure hydrogel can prevent 99% of bacterial infections.

[0053] The specific implementation process is as follows:

[0054] First, the preparation module uses an enzyme cross-linking method to design and prepare a porous hydrogel that simulates the three-dimensional structure of the extracellular matrix of natural cartilage. The porosity of the porous hydrogel is controlled at 90%. This step ensures that the hydrogel has a microstructure and mechanical properties similar to natural cartilage.

[0055] Subsequently, the preparation module adds a variety of functional materials to the hydrogel, including gelatin and chitosan with self-healing ability, growth factors TGF-β3 and BMP-2, BMP and vitamin D3 that promote bone regeneration, lubricants hyaluronic acid and glycerol, conductive materials carbon nanotubes and graphene, and antibacterial agents silver nanoparticles and antimicrobial peptides. The addition of these materials is intended to give the hydrogel a variety of biological activities and physical properties to meet the comprehensive needs of cartilage repair.

[0056] Next, the 3D unit uses 3D scanning modeling technology to collect 3D simulation information of the porous structure hydrogel, which includes porous structure characteristics, material properties, cell behavior simulation, dynamic changes in reactions, temperature, pH value, and oxygen concentration. This information provides basic data support for the construction of a 3D cartilage repair simulation system. Through the 3D cartilage repair simulation system, the 3D unit can simulate and monitor the swelling, shrinkage, biodegradation, and changes in cell behavior reactions inside the hydrogel. The simulation results are fed back to the preparation module in real time to guide the adjustment of preparation parameters.

[0057] The structural unit uses a three-dimensional cartilage repair simulation system to simulate and monitor the internal structural changes of the porous hydrogel during the preparation process, especially the pore structure stability and cross-linking degree. The simulation results are used to guide the preparation module to adjust the cross-linking agent concentration and cross-linking time to ensure that the compressive strength of the porous hydrogel reaches 1.5MPa, meeting the mechanical performance requirements of cartilage repair.

[0058] The subchondral bone unit uses a three-dimensional cartilage repair simulation system to simulate the effect of porous structure hydrogel on subchondral bone regeneration. By adjusting the concentration and reaction time of growth factors and bone regeneration factors, the GAG ​​content of the subchondral bone regeneration tissue is optimized to 30 mg / g.

[0059] The mechanical unit uses a three-dimensional cartilage repair simulation system to simulate the mechanical properties of the porous structure hydrogel, so that the mineralization degree of the lower bone regeneration tissue is 70%, ensuring that the porous structure hydrogel can remain stable when bearing joint loads.

[0060] The self-healing unit uses a three-dimensional cartilage repair simulation system to simulate the self-healing ability of porous structure hydrogels, and derives the self-healing efficiency after damage, ensuring that the self-healing efficiency is 90%.

[0061] The lubrication unit uses a three-dimensional cartilage repair simulation system to simulate the lubrication properties of porous structure hydrogels, so that the friction coefficient of the porous structure hydrogel is 0.01, ensuring that friction can be reduced and the joint surface can be protected during joint movement.

[0062] The conductive unit uses a three-dimensional cartilage repair simulation system to simulate the conductive properties of the porous structure hydrogel, so that the conductivity of the porous structure hydrogel reaches 3S / cm, allowing the porous structure hydrogel to promote cell signal transmission and cell regeneration.

[0063] The antibacterial unit uses a three-dimensional cartilage repair simulation system to simulate the antibacterial properties of the porous structure hydrogel and simulate the inhibitory effect on different pathogens, so that the porous structure hydrogel can prevent 99% of bacterial infections and ensure that the porous structure hydrogel can effectively prevent infection after being implanted in the body.

[0064] Finally, the cartilage repair hydrogel preparation management system achieved comprehensive optimization of the porous structure hydrogel preparation process through highly integrated simulation modules and real-time feedback mechanisms. The synergy between the units ensured that the hydrogel achieved optimal configuration in terms of porosity, cross-linking structure, bioactivity, mechanical properties, self-healing ability, lubrication properties, conductivity and antibacterial properties, thereby improving the effect and success rate of cartilage repair.

[0065] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0066] The above is only an embodiment of the present invention. The common sense such as the known specific structure and characteristics in the scheme is not described in detail here. The ordinary technicians in the relevant field know all the common technical knowledge in the technical field of the invention before the application date or priority date, can obtain all the existing technologies in the field, and have the ability to apply the conventional experimental means before that date. The ordinary technicians in the relevant field can improve and implement this scheme in combination with their own abilities under the enlightenment given by this application. Some typical known structures or known methods should not become obstacles for ordinary technicians in the relevant field to implement this application. It should be pointed out that for those skilled in the art, without departing from the structure of the present invention, several deformations and improvements can be made, which should also be regarded as the protection scope of the present invention, which will not affect the effect of the implementation of the present invention and the practicality of the patent. The protection scope required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. A cartilage repair hydrogel preparation and management system, characterized by: It includes preparation module and simulation module; The preparation module is used to prepare porous structure hydrogel with a porosity of 80%-90%; The simulation module is used to build an integrated three-dimensional cartilage repair simulation system; The simulation modules include three-dimensional unit, structural unit, lower bone unit, mechanical unit, self-healing unit, lubrication unit, conductive unit and antibacterial unit; The three-dimensional unit is used to collect three-dimensional simulation information of the porous structure hydrogel, and the three-dimensional simulation information is used to assist the simulation module in building a three-dimensional cartilage repair simulation system. The three-dimensional unit simulates and monitors the simulation results of the internal reaction changes of the porous structure hydrogel through the three-dimensional cartilage repair simulation system; after the three-dimensional unit obtains the simulation results of the internal reaction changes, it feeds back the simulation results of the internal reaction changes to the preparation module; The structural unit is used to simulate and monitor the internal structural change simulation results of the hydrogel during the preparation process through a three-dimensional cartilage repair simulation system. The internal structural change simulation results include pore structure stability and cross-linking degree; After the structural unit obtains the simulation results of the internal structure changes, it feeds the simulation results of the internal structure changes back to the preparation module.

2. The cartilage repair hydrogel preparation and management system according to claim 1, characterized in that: In the preparation module, the preparation method of the porous structure hydrogel includes the following steps: Step 1, simulating the cross-linked structure of the porous hydrogel into the three-dimensional structure of the natural cartilage extracellular matrix; The cross-linked structure was prepared by enzymatic cross-linking method; Step 2, using materials with self-healing ability to prepare porous structure hydrogel; the materials with self-healing ability include gelatin and chitosan; Step 3, adding growth factors into the porous structure hydrogel; the growth factors include TGF-β3 and BMP-2; Step 4, adding bone regeneration factors into the porous structure hydrogel; the bone regeneration factors include BMP and vitamin D3; Step 5, adding a lubricant to the porous structure hydrogel; the lubricant includes hyaluronic acid and glycerol; Step six, adding conductive material into the porous structure hydrogel; Conductive materials include carbon nanotubes and graphene; Step seven, adding antimicrobial agents into the porous structure hydrogel; the antimicrobial agents include silver nanoparticles and antimicrobial peptides.

3. The cartilage repair hydrogel preparation and management system according to claim 2, characterized in that: The 3D simulation information in the 3D cell includes porous structure characteristics, material properties, cell behavior simulation, reaction dynamics, temperature, pH value and oxygen concentration.

4. The cartilage repair hydrogel preparation and management system according to claim 3, characterized in that: After the structural unit feeds back the structural change simulation results to the preparation module, the preparation module adjusts the cross-linking agent concentration and cross-linking time in the enzyme cross-linking method. The compressive strength of the porous structure hydrogel prepared by the enzyme cross-linking method is 0.5-1.5MPa.

5. The cartilage repair hydrogel preparation and management system according to claim 4, characterized in that: The lower bone unit is used to simulate the lower bone regeneration simulation results of cartilage by porous structure hydrogel through a three-dimensional cartilage repair simulation system; after the lower bone unit obtains the lower bone regeneration simulation results, the lower bone unit feeds back the lower bone regeneration simulation results to the preparation module, and the preparation module adjusts the concentration and reaction time of TGF-β3, BMP-2, BMP and vitamin D3, and the GAG ​​content of the lower bone regeneration tissue is 20-30 mg / g.

6. The cartilage repair hydrogel preparation and management system according to claim 5, characterized in that: The mechanical unit is used to simulate the mechanical properties of porous structure hydrogels through a three-dimensional cartilage repair simulation system. After obtaining the mechanical properties simulation results, the mechanical unit feeds back the mechanical properties simulation results to the preparation module. The preparation module adjusts the concentration and reaction time of TGF-β3, BMP-2, BMP and vitamin D3 according to the mechanical properties simulation results. The mineralization degree of the bone regeneration tissue is 50%-70%.

7. The cartilage repair hydrogel preparation and management system according to claim 6, characterized in that: The self-healing unit is used to simulate the self-healing ability of porous structure hydrogels through a three-dimensional cartilage repair simulation system; After the self-healing unit obtains the self-healing ability simulation results, it feeds back the self-healing ability simulation results to the preparation module. The preparation module adjusts the overall proportion and reaction time of gelatin and chitosan according to the self-healing ability simulation results. The self-healing efficiency of the porous structure hydrogel is 80%-90%.

8. The cartilage repair hydrogel preparation and management system according to claim 7, characterized in that: The lubrication unit is used to simulate the lubrication performance simulation results of the porous structure hydrogel through a three-dimensional cartilage repair simulation system; after the lubrication unit obtains the lubrication performance simulation results, it feeds back the lubrication performance simulation results to the preparation module, and the preparation module adjusts the concentration of hyaluronic acid and glycerol and the reaction time according to the lubrication performance simulation results. The friction coefficient of the porous structure hydrogel is 0.01-0.

05.

9. The cartilage repair hydrogel preparation and management system according to claim 8, characterized in that: The conductive unit is used to simulate the conductive performance of the hydrogel through a three-dimensional cartilage repair simulation system. After obtaining the conductive performance simulation results, the conductive unit feeds back the conductive performance simulation results to the preparation module. The preparation module adjusts the proportion and concentration of carbon nanotubes and graphene according to the conductive performance simulation results. The conductivity of the porous structure hydrogel is 1S / cm-3S / cm.

10. The cartilage repair hydrogel preparation and management system according to claim 9, characterized in that: The antibacterial unit is used to simulate the antibacterial properties of hydrogels through a three-dimensional cartilage repair simulation system; After the antibacterial unit obtains the antibacterial performance simulation results, it feeds back the antibacterial performance simulation results to the preparation module. The preparation module adjusts the concentration and reaction time of silver nanoparticles and antibacterial peptides according to the antibacterial performance simulation results. The porous structure hydrogel has a prevention rate of pathogenic bacteria infection of 95%-99%.

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