Establishment method and application of molecular identity card of non-heading Chinese cabbage 'Shenkuqing No.1'

Abstract

The invention discloses an establishment method and application of a molecular identity card of non-heading Chinese cabbage 'Shenkuqing No.1'. PCR amplification and sequencing are carried out on DNA of the 'Shenkuqing No.1' variety through a marker combination composed of 30 pairs of SSR primers, detection is carried out at the same time, and the molecular identity card of the 'Shenkuqing No.1' variety is established through data integration of different sites according to the relative position of an amplification product. The SSR fingerprints of the 'Shenkang Qing No.1' and the parent thereof and the 'Suzhou Qing' variety are formed. Meanwhile, a PCR amplification technology and a capillary electrophoresis detection technology are used for detecting the 'Shenzuqing No.1' variety. The method solves the problems that the existing traditional morphological and cytological identification method is large in test workload and difficult to accurately and truly identify the variety type of the 'Shenkuqing No.1', and realizes the distinguishing of the 'Shenkuqing No.1' from other non-heading Chinese cabbage varieties.

Description

Technical Field

[0001] The invention relates to the establishment of a molecular identity card, in particular to an establishment method and application of a molecular identity card of non-heading Chinese cabbage 'Shenkangqing No. 1', belonging to the technical field of Chinese cabbage. Background Art

[0002] Non-heading cabbage, also known as green cabbage, Chinese cabbage, and rapeseed, has become the third most planted vegetable in China after peppers and Chinese cabbage since 2018. The cultivation area of ​​green cabbage varieties in non-heading cabbage has increased from 533,300 hectares in 2005 to 1.5 million hectares in 2017. 2 Increased to 1.3333 million hm2 in 2020 2 It has increased by about 2.5 times and played an extremely important role in the annual production and supply of vegetables.

[0003] Considering the high incidence of clubroot in cruciferous crops, cultivating new and superior varieties resistant to clubroot is one of the most effective ways to effectively control the occurrence of clubroot. Among them, 'Shenkangqing No. 1' is a hybrid variety, medium-dust type, upright, strong growth, green leaves, oval, slightly wrinkled leaves, medium-long, flat, thick, light green petioles, good waist, resistant to clubroot, can be harvested 20 to 40 days after sowing. It has the characteristics of strong growth, high yield, and excellent commodity shape.

[0004] The existing traditional morphological and cytological identification methods have a large workload and it is difficult to accurately and truly identify the differences between the varieties of 'Shen Kangqing No. 1'. It is urgent to provide a method for establishing and applying a molecular identity card for the non-heading cabbage 'Shen Kangqing No. 1'. Summary of the invention

[0005] In order to solve the shortcomings of the above-mentioned technology, the present invention provides a method for establishing a molecular identity card of non-heading Chinese cabbage 'Shenkangqing No. 1' and its application.

[0006] In order to solve the above technical problems, the technical solution adopted by the present invention is: a method for establishing a molecular identity card of non-heading Chinese cabbage 'Shenkangqing No. 1', comprising the following establishment methods:

[0007] S1. Extract the DNA of the 'Shen Kang Qing No. 1' variety, and use 30 pairs of specific primers in the primer combination to perform PCR amplification on the DNA of the 'Shen Kang Qing No. 1' variety respectively;

[0008] S2, sequencing the amplified products one by one;

[0009] S3, sequencing and testing are performed simultaneously, and according to the relative positions of the amplified products, the SSR fingerprints of 'Shenkangqing No. 1', its parents and 'Suzhouqing' are formed by integrating the data of different sites;

[0010] The established molecular ID includes primer number-primer name, molecular marker, primer sequence and fingerprint code information;

[0011] Wherein, the primer sequence includes 30 pairs of designed specific primer pairs, and PCR amplification is performed based on the specific primer pairs and molecular markers;

[0012] The fingerprint code was obtained by sequencing the PCR amplification product, including the molecular ID fingerprint code of 'Shenkangqing No. 1' and its parents and the control variety 'Suzhouqing'. The fingerprint information is as follows:

[0013]

[0014]

[0015] A1 refers to 17-1g-1, one of the parents of 'Shen Kangqing 1', B refers to 'Shen Kangqing 1', A2 refers to 17-1g-10, one of the parents of 'Shen Kangqing 1', and C refers to 'Suzhou Qing'.

[0016] Preferably, the primer numbers and primer names are SA000189-cnu_m139a, SA000190-nia_m086a, SA000191-nia_m098a, SA000192-nia_m138a, SA000193-cnu_m046a, SA000194-nia_m121a, SA000195-cnu_ m073a, SA000196-cnu_m288a, SA000197-cnu_m316a, SA000198-cnu_m327a, SA000199-n ia_m101a, SA000200-cnu_m252a, SA000201-Na10-D09, SA000202-cnu_m289a, SA000203 -cnu_m442a, SA000204-cnu_m050a, SA000205-cnu_m149a, SA000206-nia_m037a, SA00 0207-nia_m049a, SA000208-cnu_m182a, SA000209-cnu_m295a, SA000210-cnu_m090a, S A000211-cnu_m537a, SA000212-cnu_m016a, SA000213-cnu_m530a, SA000214-cnu_m534 a, SA000215-nia_m022a, SA000216-nia_m038a, SA000217-ENA2, SA000218-nia_m035a.

[0017] Preferably, the molecular markers added to the primers are obtained by amplification of the following primer pairs: SEQ ID NO.1-2, SEQ ID NO.3-4, SEQ ID NO.5-6, SEQ ID NO.7-8, SEQ ID NO.9-10, SEQ ID NO.11-12, SEQ ID NO.13-14, SEQ ID NO.15-16, SEQ ID NO.17-18, SEQ ID NO.19-20, SEQ ID NO.21-22, SEQ ID NO.23-24, SEQ ID NO.25-26, SEQ ID NO.27-28, SEQ ID NO.29-30, SEQ ID NO.31-32, SEQ ID NO.33-34, SEQ ID NO.35-36, SEQ ID NO.37-38, SEQ ID NO.39-40, SEQ ID NO.41-42, SEQ ID NO.43-44, SEQ ID NO.45-46, SEQ ID NO. Primer pairs shown in SEQ ID NO.47-48, SEQ ID NO.49-50, SEQ ID NO.51-52, SEQ ID NO.53-54, SEQ ID NO.55-56, SEQ ID NO.57-58, and SEQ ID NO.59-60.

[0018] Preferably, the 30 pairs of specific primer pairs are: SEQ ID NO.1-2, SEQ ID NO.1-2, SEQ ID NO.3-4, SEQ ID NO.5-6, SEQ ID NO.7-8, SEQ ID NO.9-10, SEQ ID NO.11-12, SEQ ID NO.13-14, SEQ ID NO.15-16, SEQ ID NO.17-18, SEQ ID NO.19-20, SEQ ID NO.21-22, SEQ ID NO.23-24, SEQ ID NO.25-26, SEQ ID NO.27-28, SEQ ID NO.29-30, SEQ ID NO.31-32, SEQ ID NO.33-34, SEQ ID NO.35-36, SEQ ID NO.37-38, SEQ ID NO.39-40, SEQ ID NO.41-42, SEQ ID NO.43-44, SEQ ID NO.45-46, SEQ ID NO. NO.47-48, SEQ ID NO.49-50, SEQ ID NO.51-52, SEQ ID NO.53-54, SEQ ID NO.55-56, SEQ ID NO.57-58, SEQ ID NO.59-60.

[0019] As a preferred embodiment, the fingerprint information is formed by integrating the data of different sites according to the relative position of the amplified product, specifically: when the amplified product reads the band as none, the dominant marker is recorded as 0; when the amplification condition is yes, the dominant marker is recorded as 1; when the band is weak or the genotype cannot be determined, it is recorded as?, and the invalid band is recorded as--; the allele variation size data of the homozygous site in the co-dominant marker is recorded as X / X, the amplified product reads the band as homozygous parent 1, where X is the size of the allele variation of the site; the allele variation size data of the homozygous site is recorded as Y / Y, the amplified product reads the band as homozygous parent 2, and Y is the size of the allele variation of the site; the allele variation data of the heterozygous site is recorded as X / Y, the amplified product is a parental band type, where X and Y are two different allele variations at the site; when the band is weak or the genotype cannot be determined, it is recorded as?, and the size of the invalid allele variation is recorded as--; the " / " before the above is a small fragment, and the " / " after the large fragment.

[0020] Preferably, fingerprint QR codes are generated for 'Shenkangqing No. 1' and its parent and control variety 'Suzhouqing', respectively.

[0021] The invention discloses an application method of a molecular ID card of non-heading Chinese cabbage 'Shenkangqing No. 1', comprising the following steps: extracting DNA of a sample to be identified, performing PCR amplification and detecting PCR products by capillary electrophoresis to identify the variety of the sample to be detected.

[0022] The invention discloses a method for establishing a molecular ID card of non-heading Chinese cabbage 'Shen Kang Qing No. 1' and its application, a marker combination consisting of 30 pairs of SSR primers, and the 'Shen Kang Qing No. 1' variety is detected by using PCR amplification technology and capillary electrophoresis detection technology, which can complete the identification of the 'Shen Kang Qing No. 1' variety and genetic diversity evaluation work in a short time, and has the advantages of time saving, high efficiency, rapidity, accuracy, and convenient operation. The method for establishing the molecular ID card of the non-heading Chinese cabbage 'Shen Kang Qing No. 1' can effectively monitor the authenticity of the 'Shen Kang Qing No. 1' variety, reveal the genetic variation and genetic relationship of the variety from the DNA level, protect the crop varieties, and prevent counterfeit and inferior products from entering the market. DETAILED DESCRIPTION

[0023] The present invention is further described in detail below in conjunction with specific implementation modes.

[0024] The present invention provides a method for establishing a molecular identity card of non-heading Chinese cabbage 'Shen Kang Qing No. 1', which solves the problem that the existing traditional morphological and cytological identification methods have a large testing workload and are difficult to accurately and truly identify the variety type of 'Shen Kang Qing No. 1', and achieves differentiation from other non-heading Chinese cabbage varieties.

[0025] The embodiment of the present invention provides a method for establishing a fingerprint of non-heading Chinese cabbage 'Shenkangqing No. 1', wherein the established molecular ID card includes information such as material name, material characteristic properties, primer sequence, fingerprint code and QR code;

[0026] First, the primer number-primer name was designed. The primer number-primer name was SA000189-cnu_m139a, SA000190-nia_m086a, SA000191-nia_m098a, SA000192-nia_m138a, SA000193-cnu_m046a, SA000194-nia_m121a, SA0001 95-cnu_m073a, SA000196-cnu_m288a, SA000197-cnu_m316a, SA000198-cnu_m327a, SA00 0199-nia_m101a, SA000200-cnu_m252a, SA000201-Na10-D09, SA000202-cnu_m289a, SA0 00203-cnu_m442a, SA000204-cnu_m050a, SA000205-cnu_m149a, SA000206-nia_m037a, S A000207-nia_m049a, SA000208-cnu_m182a, SA000209-cnu_m295a, SA000210-cnu_m090a , SA000211-cnu_m537a, SA000212-cnu_m016a, SA000213-cnu_m530a, SA000214-cnu_m53 4a, SA000215-nia_m022a, SA000216-nia_m038a, SA000217-ENA2, SA000218-nia_m035a;

[0027] The molecular markers were amplified by the following primer pairs: SEQ ID NO.1-2, SEQ ID NO.3-4, SEQ ID NO.5-6, SEQ ID NO.7-8, SEQ ID NO.9-10, SEQ ID NO.11-12, SEQ ID NO.13-14, SEQ ID NO.15-16, SEQ ID NO.17-18, SEQ ID NO.19-20, SEQ ID NO.21-22, SEQ ID NO.23-24, SEQ ID NO.25-26, SEQ ID NO.27-28, SEQ ID NO.29-30, SEQ ID NO.31-32, SEQ ID NO.33-34, SEQ ID NO.35-36, SEQ ID NO.37-38, SEQ ID NO.39-40, SEQ ID NO.41-42, SEQ ID NO.43-44, SEQ ID NO.45-46, SEQ ID NO.47-48, SEQ ID NO. The primer pairs shown in SEQ ID NO.49-50, SEQ ID NO.51-52, SEQ ID NO.53-54, SEQ ID NO.55-56, SEQ ID NO.57-58, and SEQ ID NO.59-60 were used to obtain the molecular markers of 'Shenkangqing No. 1' and its parents.

[0028] The designed primer combination consists of the following 30 pairs of specific primer pairs, which are SEQ ID NO.1-2, SEQ ID NO.1-2, SEQ ID NO.3-4, SEQ ID NO.5-6, SEQ ID NO.7-8, SEQ ID NO.9-10, SEQ ID NO.11-12, SEQ ID NO.13-14, SEQ ID NO.15-16, SEQ ID NO.17-18, SEQ ID NO.19-20, SEQ ID NO.21-22, SEQ ID NO.23-24, SEQ ID NO.25-26, SEQ ID NO.27-28, SEQ ID NO.29-30, SEQ ID NO.31-32, SEQ ID NO.33-34, SEQ ID NO.35-36, SEQ ID NO.37-38, SEQ ID NO.39-40, SEQ ID NO.41-42, SEQ ID NO.43-44, SEQ ID NO.45-46, SEQ ID NO.47-48, SEQ ID NO.49-50, SEQ ID NO.51-52, SEQ ID NO.53-54, SEQ ID NO.55-56 NO.45-46, SEQ ID NO.47-48, SEQ ID NO.49-50, SEQ ID NO.51-52, SEQ ID NO.53-54, SEQ ID NO.55-56, SEQ ID NO.57-58, SEQ ID NO.59-60, specify the primer combination information in detail.

[0029] Example 1

[0030] A kit for the combination of molecular markers and primers, and the PCR amplification product is detected by the kit as follows:

[0031] When the amplification product reads no band, it is recorded as 0 when it is a dominant marker; when the amplification condition is yes, the dominant marker is recorded as 1; when the band is weak or the genotype cannot be determined, it is recorded as?, and the invalid band is recorded as--; the allele variation size data of the homozygous site in the co-dominant marker is recorded as X / X, and the amplification product reads the homozygous parent 1, where X is the size of the allele variation of the site; the allele variation size data of the homozygous site is recorded as Y / Y, and the amplification product reads the homozygous parent 2, where Y is the size of the allele variation of the site; the allele variation data of the heterozygous site is recorded as X / Y, and the amplification product is a parental band type, where X and Y are two different allele variations at the site; when the band is weak or the genotype cannot be determined, it is recorded as?, and the size of the invalid allele variation is recorded as--; the " / " before the above is a small fragment, and the " / " after the large fragment; by integrating the data of different sites, the SSR fingerprint code of 'Shenkangqing No. 1' and its parents and the control variety 'Suzhouqing' was formed.

[0032] The fingerprint information of 'Shenkangqing No. 1', its parents and 'Suzhouqing' are as follows:

[0033]

[0034]

[0035]

[0036] A refers to one of the parents of Shenkangqing No. 1, 17-1g-1; B refers to Suzhouqing; A2 refers to the other parent of Shenkangqing No. 1, 17-1g-10; and C refers to Shenkangqing No. 1.

[0037] The application of molecular markers and primer combinations in identifying the cabbage variety 'Shenkangqing No. 1', wherein the PCR amplification method is:

[0038] The reaction program was as follows: pre-denaturation at 94°C for 4 min; denaturation at 94°C for 45 s, annealing at 65°C for 40 s, extension at 72°C for 45 s, with a 1°C decrease in each cycle, for a total of 15 cycles; denaturation at 94°C for 45 s, annealing at 50°C for 30 s, extension at 72°C for 45 s, for a total of 30 cycles; extension at 72°C for 10 min, and insulation at 4°C;

[0039] Reaction system: 25 μL reaction system volume, containing 0.25 mmol / L of each dNTP, 0.4 μmol / L of forward primer and reverse primer, 1.0 U of Taq DNA polymerase, 1× PCR buffer (without Mg2+), 1.5 mmol / L of MgCl2, and 10-40 ng of sample DNA.

[0040] Application of molecular markers and primer combinations in identifying the parents of 'Shenkangqing 1' and 'Shenkangqing 1'.

[0041] The primer combination was used to perform PCR amplification on the DNA of 'Shenkangqing No. 1' and its parents and the PCR product was detected by capillary electrophoresis. Fluorescence capillary electrophoresis was used for detection and fluorescent markers were added to the primers. The details are as follows:

[0042]

[0043]

[0044]

[0045] In Example 1, the fingerprint sequence of 'Shen Kang Qing No. 1' and its parents was obtained, and the different varieties of cabbage were compared with each other to analyze their differences. The size difference between the band types of SSR primer amplification products was obvious, forming a unique DNA fingerprint, which can well distinguish different varieties. The molecular ID card of the present invention can effectively identify the variety of 'Shen Kang Qing No. 1', and has the advantages of high efficiency, accuracy, low cost, and simple operation.

[0046] Furthermore, for ease of use, fingerprint QR codes were generated for 'Shenkangqing No. 1' and its parent and control variety 'Suzhouqing'.

[0047] Example 2

[0048] A molecular identification method for the variety 'Shenkangqing No. 1' comprises the following steps:

[0049] S1. Extract DNA of the 'Shenkangqing No. 1' variety, and perform PCR amplification on the DNA of the 'Shenkangqing No. 1' variety using 30 pairs of SSR primers in the primer combination;

[0050] S2, performing first-generation sequencing on the amplified product;

[0051] S3. Detection was performed while sequencing. According to the relative position of the amplified products, the SSR fingerprint of 'Shenkangqing No. 1' and its parents and 'Suzhouqing' was formed by integrating the data of different sites. When the amplified product read band was absent, it was recorded as 0 when it was a dominant marker; when the amplification condition was yes, the dominant marker was recorded as 1; when the band was weak or the genotype could not be determined, it was recorded as?, and the invalid band was recorded as--; the allele variation size data of the homozygous site in the co-dominant marker was recorded as X / X, and the amplified product read band was homozygous parent 1, where X was the size of the allele variation of the site; the allele variation size data of the homozygous site was recorded as Y / Y, and the amplified product read band was homozygous parent 2, where Y was the size of the allele variation of the site; the allele variation data of the heterozygous site was recorded as X / Y, and the amplified product was a parental band type, where X and Y were two different allele variations at the site; when the band was weak or the genotype could not be determined, it was recorded as?. The size of the invalid allele variation is recorded as --; the fragments before the “ / ” are small fragments, and the fragments after the “ / ” are large fragments.

[0052] In this Example 2, the molecular ID card of the present invention can effectively monitor the authenticity of 'Shenkangqing No. 1' and its parent seeds, protect the species, and prevent counterfeit and inferior varieties from entering the market.

[0053] The above implementation modes are not limitations of the present invention, and the present invention is not limited to the above examples. Changes, modifications, additions or substitutions made by technicians in this technical field within the scope of the technical solution of the present invention also belong to the protection scope of the present invention.

Claims

1. A method for establishing a molecular identity card of non-heading Chinese cabbage 'Shenkangqing No. 1', characterized in that: The following methods are included: S1. Extract the DNA of the 'Shen Kang Qing No. 1' variety, and perform PCR amplification on the DNA of the 'Shen Kang Qing No. 1' variety using 30 pairs of specific primers in the primer combination; S2, sequencing the amplified products one by one; S3, sequencing and testing are performed simultaneously, and according to the relative positions of the amplified products, the SSR fingerprints of 'Shenkangqing No. 1', its parents and 'Suzhouqing' are formed by integrating the data of different sites; The established molecular ID includes primer number-primer name, molecular marker, primer sequence and fingerprint code information; Wherein, the primer sequence includes 30 pairs of designed specific primer pairs, and PCR amplification is performed based on the specific primer pairs and molecular markers; The fingerprint code is obtained by sequencing the PCR amplification product, and includes the molecular ID fingerprint code of 'Shenkangqing No. 1' and its parents and the control variety 'Suzhouqing'. The fingerprint information is as follows: A1 refers to 17-1g-1, one of the parents of 'Shen Kangqing 1', B refers to 'Shen Kangqing 1', A2 refers to 17-1g-10, one of the parents of 'Shen Kangqing 1', and C refers to 'Suzhou Qing'.

2. The method for establishing the molecular identity card of the non-heading Chinese cabbage 'Shen Kangqing No. 1' according to claim 1, characterized in that: The primer numbers and primer names are SA000189-cnu_m139a, SA000190-nia_m086a, SA000191-nia_m098a, SA000192-nia_m138a, SA000193-cnu_m046a, SA000194-nia_m121a, and SA000195-cnu_m07 3a, SA000196-cnu_m288a, SA000197-cnu_m316a, SA000198-cnu_m327a, SA000199-nia _m101a, SA000200-cnu_m252a, SA000201-Na10-D09, SA000202-cnu_m289a, SA000203-c nu_m442a, SA000204-cnu_m050a, SA000205-cnu_m149a, SA000206-nia_m037a, SA0002 07-nia_m049a, SA000208-cnu_m182a, SA000209-cnu_m295a, SA000210-cnu_m090a, SA 000211-cnu_m537a, SA000212-cnu_m016a, SA000213-cnu_m530a, SA000214-cnu_m534 a, SA000215-nia_m022a, SA000216-nia_m038a, SA000217-ENA2, SA000218-nia_m035a.

3. The method for establishing the molecular identity card of the non-heading Chinese cabbage 'Shen Kangqing No. 1' according to claim 2, characterized in that: The molecular markers added to the primers are obtained by amplification of the following primer pairs: SEQ ID NO.1-2, SEQ ID NO.3-4, SEQ ID NO.5-6, SEQ ID NO.7-8, SEQ ID NO.9-10, SEQ ID NO.11-12, SEQ ID NO.13-14, SEQ ID NO.15-16, SEQ ID NO.17-18, SEQ ID NO.19-20, SEQ ID NO.21-22, SEQ ID NO.23-24, SEQ ID NO.25-26, SEQ ID NO.27-28, SEQ ID NO.29-30, SEQ ID NO.31-32, SEQ ID NO.33-34, SEQ ID NO.35-36, SEQ ID NO.37-38, SEQ ID NO.39-40, SEQ ID NO.41-42, SEQ ID NO.43-44, SEQ ID NO.45-46, SEQ ID NO. Primer pairs shown in SEQ ID NO.47-48, SEQ ID NO.49-50, SEQ ID NO.51-52, SEQ ID NO.53-54, SEQ ID NO.55-56, SEQ ID NO.57-58, and SEQ ID NO.59-60.

4. The method for establishing the molecular identity card of the non-heading Chinese cabbage 'Shen Kangqing No. 1' according to claim 3, characterized in that: The 30 specific primer pairs are: SEQ ID NO.1-2, SEQ ID NO.1-2, SEQ ID NO.3-4, SEQ ID NO.5-6, SEQ ID NO.7-8, SEQ ID NO.9-10, SEQ ID NO.11-12, SEQ ID NO.13-14, SEQ ID NO.15-16, SEQ ID NO.17-18, SEQ ID NO.19-20, SEQ ID NO.21-22, SEQ ID NO.23-24, SEQ ID NO.25-26, SEQ ID NO.27-28, SEQ ID NO.29-30, SEQ ID NO.31-32, SEQ ID NO.33-34, SEQ ID NO.35-36, SEQ ID NO.37-38, SEQ ID NO.39-40, SEQ ID NO.41-42, SEQ ID NO.43-44, SEQ ID NO.45-46, SEQ ID NO.47-48, SEQ ID NO.49-50, SEQ ID NO.51-52, SEQ ID NO.53-54, SEQ ID NO.55-56, SEQ ID NO.57-58, SEQ ID NO.59-60.

5. The method for establishing the molecular identity card of the non-heading Chinese cabbage 'Shen Kangqing No. 1' according to claim 1, characterized in that: The fingerprint information is formed by integrating the data of different sites according to the relative position of the amplified products. Specifically, when the amplified product reads the band as no, the dominant marker is recorded as 0; when the amplification condition is yes, the dominant marker is recorded as 1; when the band is weak or the genotype cannot be determined, it is recorded as?, and the invalid band is recorded as--; the allele variation size data of the homozygous site in the co-dominant marker is recorded as X / X, and the amplified product reads the band as homozygous parent 1, where X is the size of the allele variation of the site; the allele variation size data of the homozygous site is recorded as Y / Y, and the amplified product reads the band as homozygous parent 2, where Y is the size of the allele variation of the site; the allele variation data of the heterozygous site is recorded as X / Y, and the amplified product is a parental band type, where X and Y are two different allele variations at the site; when the band is weak or the genotype cannot be determined, it is recorded as?, and the size of the invalid allele variation is recorded as--; the " / " before the above is a small fragment, and the " / " after the large fragment.

6. The method for establishing the molecular identity card of the non-heading Chinese cabbage 'Shen Kangqing No. 1' according to claim 1, characterized in that: Fingerprint QR codes were generated for 'Shenkangqing No. 1' and its parent and control variety 'Suzhouqing'.

7. An application method of a molecular ID card of non-heading Chinese cabbage 'Shenkangqing No. 1', characterized in that: The method comprises the following steps: extracting DNA of a sample to be identified, performing PCR amplification and detecting PCR products by capillary electrophoresis to identify the species of the sample to be detected.

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