Modeling device for fixing head of newborn mouse and performing supplementary anesthesia
Through the symmetrical setting of ear rod assembly and tail-flipped supplementary anesthesia mechanism, the problems of head fixation and anesthesia supplement in mice were solved, and the effect of automatic supplementary anesthesia and improving experimental efficiency was achieved.
Patent Information
- Application Number
- CN202411090551.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-09
- Publication Date
- 2024-12-13
AI Technical Summary
The existing mouse modeling device has shortcomings in head fixation and anesthesia supplementation. The head fixation is not firm enough. After the anesthetic effect disappears, mice struggle easily damage blood vessels, and experimental personnel with rich experience need to frequently observe and supplement anesthesia. , low work efficiency.
A molding device including a symmetrical arrangement of ear rod assembly and tail-flip-floping supplementary anesthesia mechanism was designed to fix the mouse's head through an airbag, and automatically supplementary anesthesia in mice is achieved using a tail clamp and a hydraulic amplification chamber, combined with artificial supplementation. Reduce personnel energy consumption and improve anesthesia control.
The head fixation effect of mice is improved, the damage caused by the struggle of mice during anesthesia is reduced, and automatic supplementary anesthesia is achieved, which improves experimental efficiency and safety.
Smart Images
Figure CN119112422A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of animal model making tools, and in particular to a model making device for fixing the head of a newborn mouse and performing supplementary anesthesia. Background Art
[0002] Neonatal mice refer to mice that have just been born, usually young mice from shortly after birth to the weaning stage. At this stage, the physiological and behavioral characteristics of mice are significantly different from those of adult mice, including the need for breastfeeding, sensitivity to environmental temperature, and incomplete development. Studying neonatal mice is of great significance for understanding their growth and development, neurodevelopment, behavior, and metabolism, and is widely used in life science research.
[0003] Mouse modeling is a commonly used experimental method in biomedical research. It aims to simulate specific human diseases or pathological conditions in order to deeply study their occurrence, development and treatment mechanisms. Mouse modeling can provide a deeper understanding of the pathogenesis of diseases, pathological processes and the effects of treatment methods. This method can not only help researchers screen potential therapeutic drugs, but also provide an important theoretical basis for the prevention and treatment of diseases.
[0004] During the modeling process, the mice need to be fixed, then anesthetized, and then modeling-related operations are performed. The fixation effect and anesthesia effect are directly related to the success or failure of the modeling.
[0005] There are three commonly used animal anesthesia methods in basic research: traditional inhalation anesthesia, traditional injection anesthesia and isoflurane gas anesthesia. Traditional injection anesthesia is not suitable for anesthesia of newborn mice; isoflurane gas anesthesia can easily cause harm to experimenters; traditional respiratory anesthesia time and concentration are difficult to control, but it is easy to operate and is often the first choice for experiments. Due to the young age of mice and different physical conditions, excessive anesthesia can easily lead to direct death of mice, and inadequate anesthesia will cause mice to struggle, resulting in failure of modeling or increased difficulty, so mice need to be subjected to multiple mild respiratory anesthesia.
[0006] The current mouse modeling devices have the following problems and shortcomings: ① The head is not fixed firmly enough, and the mouse's struggle after the anesthetic effect disappears is likely to damage the blood vessels, resulting in modeling failure; ② Experimenters need rich experience to grasp the degree of anesthesia of the mice, and need to frequently observe all aspects of the mice so as to replenish anesthesia in time to maintain the mice's anesthesia state.
[0007] After searching, the Chinese patent with the announcement number CN116370134A proposed a modeling device for fixing the head of newborn mice and performing supplementary anesthesia, including a modeling device for supplementary anesthesia of experimental mice: a mouse mask, a rubber head, and an anesthetic volatilizer bottle; a modeling device for fixing the head of experimental mice: an ear plug rod, a head fixing clamp, an operating table, a support frame, and bolts. The modeling device of this invention is suitable for fixing the head of experimental newborn mice and performing supplementary anesthesia. It can solve the problems of insufficient head fixation, inadequate head fixation angle, and inconvenient supplementary anesthesia in traditional modeling devices. The device is simple in structure, easy to operate, can assist in accurately fixing the angle of the head, and effectively prevent the failure of modeling due to the struggle of the mouse due to insufficient head fixation after the anesthesia effect.
[0008] Although the above device has improved the fixation of the mouse head, it does not have a corresponding detection structure and supplementary anesthesia structure for supplementary anesthesia. Manual observation and manual supplementary anesthesia are still required. The staff needs to continuously observe and judge and provide supplementary anesthesia to the mice whose anesthetic effect disappears, and the work efficiency is low. Summary of the invention
[0009] In response to the above-mentioned problems, the present invention aims to provide a modeling device for fixing the head of a newborn mouse and performing supplementary anesthesia. The designed fixing device is friendly to mice, not easy to damage mice, and has good adaptability. According to the instinctive tail-flicking reaction of mice, automatic supplementary anesthesia is performed, combined with manual supplementary anesthesia, thereby reducing personnel energy consumption and improving the control of anesthesia level.
[0010] The main idea of the technical solution adopted by the present invention is as follows: the head of the mouse is fixed by a symmetrically arranged ear rod assembly, an air bag is arranged at the front end of the ear rod, and a hand wheel is arranged at the rear end, the hand wheel rotates to drive the screw rod to move forward, and then the air bag is inflated, and the air bag is expanded, thereby increasing the adaptability of contact with the mouse ear, reducing the chance of injury, and improving the fixing effect; a tail clamp capable of clamping the mouse tail is arranged, the end of the tail clamp is connected to an elastic rope, the elastic rope drives the rotating rod to rotate, and the pulling force is initially amplified to squeeze the first spring piston rod, and then the hydraulic pipe fixed on the mounting seat three is squeezed, the tail swinging force is converted into liquid pressure, and transmitted to the hydraulic amplification chamber, in the hydraulic amplification chamber, the tail swinging force is amplified for the second time to push the second spring piston rod to move, squeeze the anesthetic gas chamber, so that the anesthetic gas enters the mask through the one-way valve, and the mouse is automatically supplemented with anesthesia.
[0011] In order to achieve the above object, the technical solution adopted by the present invention is as follows: A modeling device for fixing the head of a newborn mouse and performing supplementary anesthesia comprises an operating base and also comprises: an ear rod assembly, which is provided with two groups and symmetrically distributed on both sides of the mouse fixed on the operating base; The tail-flicking supplementary anesthesia mechanism comprises a supplementary anesthesia component and a detection and transmission component, which are arranged on an operating base. The supplementary anesthesia component and the detection and transmission component are arranged on the operating base. The detection and transmission component clamps the tail of the mouse and triggers the supplementary anesthesia component to supplement the anesthesia of the mouse by detecting the mouse's tail flick.
[0012] Through the above technical solution, further: the ear rod assembly includes a pull rod, a sleeve is sleeved on the pull rod, the sleeve is slidably connected to the pull rod, one end of the pull rod is connected to the ear rod, and the other end of the pull rod is movably connected to the screw rod.
[0013] Through the above technical solution, further: an air inlet is opened on the sleeve, an airbag inflation port is opened on the ear rod, and an airbag is arranged at the front end of the ear rod.
[0014] Through the above technical solution, further: the detection transmission component includes a tail clamp, the tail clamp is connected to an elastic rope, and the elastic rope is connected to a first amplification unit.
[0015] Through the above technical solution, further: the first amplifying unit includes a mounting seat three arranged on the operating base, the mounting seat three is provided with a rotating seat, the rotating seat is rotatably connected to a rotating rod, and the rotating rod is connected to the elastic rope.
[0016] Through the above technical solution, further: a first spring piston rod is also arranged on the mounting seat three, the first spring piston rod comprises a spring one and a piston rod, the spring is arranged on the piston rod, the spring one is connected to the mounting seat three, and the rotating rod is in contact with the piston rod.
[0017] Through the above technical solution, further: the first spring piston rod is connected to the hydraulic pipe, the other end of the hydraulic pipe is connected to the second amplification unit, the second amplification unit includes a hydraulic amplification chamber, and hydraulic grooves with different diameters are arranged in the hydraulic amplification chamber, and the hydraulic pipe is connected to the hydraulic groove with the smaller diameter.
[0018] Through the above technical solution, further: the supplementary anesthesia component includes an anesthesia chamber, and the anesthesia chamber is connected to the hydraulic amplification chamber through a second spring piston rod.
[0019] A method for using a modeling device for fixing the head of a newborn mouse and performing supplementary anesthesia, using any of the above-mentioned modeling devices for fixing the head of a newborn mouse and performing supplementary anesthesia, and the specific method is as follows: S1. Perform preliminary anesthesia on the mouse, rotate the screw to make it move forward, inflate the airbag at the same time, expand the airbag, and fix the mouse's head; S2, open the tail clamp, put the mouse tail in, and clamp the mouse tail; S3. When the anesthetic effect of the mouse weakens during the experiment, the mouse body reacts instinctively to cause the mouse to flick its tail. When the detection and transmission component detects the mouse flicking its tail, the supplementary anesthesia component provides supplementary anesthesia to the mouse.
[0020] Through the above technical solution, further: wherein S3 is specifically: When the mouse's tail swings, the tail clamp pulls the elastic rope, and the elastic rope drives the rotating rod to rotate, amplifying the pulling force of the elastic rope for the first time, squeezing the first spring piston rod, and then squeezing the hydraulic tube, converting the amplified pulling force into liquid pressure, which is transmitted to the hydraulic amplification chamber through the hydraulic tube. The force amplified for the second time in the hydraulic amplification chamber pushes the second spring piston rod to move, squeezing the anesthetic gas chamber, and supplementing anesthesia for the mouse.
[0021] The beneficial effects of the present invention are: 1. The mouse head is fixed by a symmetrically arranged ear rod assembly. An air bag is arranged at the front end of the ear rod, and a hand wheel is arranged at the rear end. The rotation of the hand wheel drives the screw rod to move forward, thereby inflating the air bag and expanding the air bag, which increases the adaptability of the contact with the mouse ear, reduces the chance of injury, and improves the fixation effect.
[0022] 2. When the anesthetic effect of mice weakens during the experiment, the mice will have an instinctive physical reaction. At this time, the stimulation of the tail by the tail clamp will cause the tail of the mouse to swing. The end of the tail clamp is connected to an elastic rope. When the tail swings, it can adapt to pulling at any angle and direction. The elastic rope drives the rotating rod to rotate, and the pulling force of the elastic rope is initially amplified to squeeze the first spring piston rod, squeeze the hydraulic pipe fixed on the mounting seat three, and convert the amplified pulling force into liquid pressure, which is transmitted to the hydraulic amplification chamber. The force amplified by the hydraulic amplification chamber for the second time pushes the second spring piston rod to move, squeeze the anesthetic gas chamber, and allow the anesthetic gas to enter the mask through the hose, thereby realizing automatic supplementary anesthesia of the mouse.
[0023] 3. An elastic and bendable fixing rod is set on one side of the mask, which can adapt to the position of the mouse's head for experiments; there is a ventilation tube with anesthesia neutralization and filtration function on the side, which can play an anesthesia neutralization and filtration role. A one-way valve is set at the end of the ventilation tube to allow air to pass through to ensure the mouse's breathing; an anesthetic gas neutralization and filtration structure is also set in the ventilation tube. After the experiment, the anesthetic gas entering the ventilation tube can be processed to make it lose its anesthetic effect, so as to avoid the overflow of anesthetic gas and cause harm to the experimenters. BRIEF DESCRIPTION OF THE DRAWINGS
[0024] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention; Figure 2 This is a schematic diagram of the three-dimensional structure of the operating base of the present invention; Figure 3 This is a schematic diagram of the three-dimensional structure of Embodiment 1 of the present invention; Figure 4 for Figure 3 Schematic diagram from top view; Figure 5 for Figure 4 Schematic diagram of the cross section along CC; Figure 6 for Figure 5 A schematic diagram of some structures; Figure 7 It is a schematic diagram of the three-dimensional structure of the ear rod assembly of the present invention; Figure 8 This is a schematic diagram of the three-dimensional structure of the pull rod connection relationship of the present invention; Fig. 9 It is a schematic diagram of the three-dimensional structure of the ear rod of the present invention; Fig.10 It is a schematic diagram of the three-dimensional structure of the sleeve of the present invention; Fig.11 This is a schematic diagram of the three-dimensional structure of the mask of the present invention; Fig.12 This is a schematic diagram of the three-dimensional structure of the second embodiment of the present invention; Fig.13 for Fig.12 A schematic diagram of some structures; Fig.14 It is a schematic diagram of the three-dimensional structure of the tail clamp and the elastic rope of the present invention; Fig.15 It is a top view schematic diagram of the present invention; Fig.16 for Fig.15 Schematic diagram of section along AA; Fig.17 for Fig.15 Schematic cross-sectional view along BB; Fig.18 for Fig.17 A schematic diagram of some structures; Among them: 1. operating base; 101. placing table; 102. connecting seat; 103. mounting seat 1; 104. mounting seat 2; 105. mounting seat 3; 2. Fixing strip; 3. Ear rod assembly; 301. Screw rod; 302. Hand wheel; 303. Pull rod; 303-1. First damping sealing sleeve; 304. Sleeve; 304-1. Pressure relief port; 304-2. Air inlet; 304-3. Second damping sealing sleeve; 305. Ear rod; 305-1. Air bag; 305-2. Air bag inflation port; 4. Mask; 401. Fixing rod; 402. Connecting tube; 403. Accommodating tube; 404. Ventilation tube; 5. Tail-flicking supplementary anesthesia mechanism; 501. rotating seat; 502. rotating rod; 503. elastic rope; 504. tail clamp; 505. first spring piston rod; 505-1. spring one; 505-2. pressing rod; 505-3. pressing cap; 505-4. pushing plate; 506. hydraulic pipe; 507. hydraulic amplification chamber; 507-1. hydraulic groove; 508. second spring piston rod; 509. anesthesia chamber; 509-1. air inlet pipe. DETAILED DESCRIPTION
[0025] In order to make the purpose, technical solution and advantages of the embodiments of the present application clearer, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. The components of the embodiments of the present application described and shown in the drawings here can be arranged and designed in various different configurations.
[0026] The inventors have found that the current mouse modeling devices have the following problems and shortcomings: ① The head is not fixed firmly enough, and the mouse's struggle after the anesthetic effect disappears is likely to damage the blood vessels, resulting in modeling failure; ② The experimenter needs rich experience to grasp the degree of anesthesia of the mouse, and needs to frequently observe all aspects of the mouse so as to replenish anesthesia in time to maintain the mouse's anesthesia state.
[0027] Based on the above findings, the present application proposes a modeling device for fixing the head of a newborn mouse and performing supplementary anesthesia, wherein the head of the mouse is fixed by a symmetrically arranged ear rod assembly, an air bag is provided at the front end of the ear rod, and a hand wheel is provided at the rear end, and the rotation of the hand wheel drives the screw rod to move forward, thereby inflating the air bag and expanding the air bag, thereby increasing the adaptability of the contact with the mouse ear, reducing the chance of injury, and improving the fixation effect; a tail clamp capable of clamping the mouse tail is provided, and an elastic rope is connected to the end of the tail clamp, and the elastic rope drives the rotating rod to rotate, and the pulling force is initially amplified to squeeze the first spring piston rod, and then squeeze the hydraulic pipe fixed on the mounting seat three, and the force of the tail swinging is converted into liquid pressure, which is transmitted to the hydraulic amplification chamber, and the force of the tail swinging in the hydraulic amplification chamber is amplified for the second time to push the second spring piston rod to move, squeeze the anesthetic gas chamber, and allow the anesthetic gas to enter the mask through the one-way valve, thereby achieving supplementary anesthesia for the mouse. Embodiment 1
[0028] See also Figure 1-Figure 10The present application discloses a modeling device for fixing the head of a newborn mouse and performing supplementary anesthesia, comprising an operating base 1, on which a placing table 101 is arranged, and the upper surface of the placing table 101 is arc-shaped, for placing the mouse, and the limbs of the mouse can be spread out on the placing table 101. Fixed bars 2 are arranged on both sides of the placing table 101, one end of the fixed bar 2 is rotatably connected to a connecting seat 102 arranged on the operating base 1, and the other end of the fixed bar 2 is provided with a structure that can be connected to each other, so that the two fixed bars 2 can be detachably connected, and multiple implementation methods such as snap connection, magnetic connection, and Velcro connection can be adopted.
[0029] Mounting seats 103 are also arranged on both sides of the placement platform 101. Two mounting seats 103 are symmetrically arranged and arranged at one end close to the mouse head. Ear rod components 3 are arranged on the mounting seat 103, and the ear rod components 3 are arranged oppositely.
[0030] The ear rod assembly 3 includes a screw rod 301 threadedly connected to the mounting seat 103, one end of the screw rod 301 is fixedly connected to a hand wheel 302, and the other end is rotatably connected to a sleeve 304, and the sleeve 304 is sleeved on the outside of the screw rod 301. A pull rod 303 is slidably connected inside the sleeve 304, and a boss is provided on the pull rod 303 to cooperate with the inner wall of the sleeve 304 for guidance. A groove is provided at one end of the pull rod 303 to cooperate with the clamping table at the front end of the screw rod 301. The pull rod 303 is rotatably connected to the screw rod 301, so that when the screw rod 301 rotates and moves, the pull rod 303 can be carried and moved without following the synchronous rotation. A first damping sealing sleeve 303-1 is also provided on the pull rod 303, which performs gas sealing on the one hand and increases the friction with the sleeve 304 on the other hand, further reducing the rotation probability of the pull rod 303.
[0031] The other end of the pull rod 303 is fixedly connected to the ear rod 305, and when the pull rod 303 slides left and right, it can drive the ear rod 305 to move synchronously. The ear rod 305 is made of a plastic material with a certain elasticity and hardness, which ensures the stability of the fixation and has a certain ability to adapt to slight deformation. The ear rod 305 is a hollow tubular structure, and an air bag 305-1 is provided at the front end. A limited position frame is provided at one end of the ear rod 305 connected to the pull rod 303.
[0032] A pressure relief port 304 - 1 is provided at the upper end of the sleeve 304 . The pressure relief port 304 - 1 is provided at the front end of the sleeve 304 , close to the ear rod 305 . The internal pressure is controlled by a one-way membrane flap, and the gas can only leak outwards but cannot be introduced inwards.
[0033] The airbag 305-1 is set to a certain shape and pressure. When a certain pressure is reached, the gas will overflow from the pressure relief port 304-1 to prevent the airbag 305-1 from exceeding the set expansion range. The airbag 305-1 at the front end of the ear rod 305 increases the adaptability of contact with the mouse ear, reduces the chance of injury, and improves the fixing effect.
[0034] The sleeve 304 has an air inlet 304-2 at the lower end, and the air inlet 304-2 is arranged in the middle of the sleeve 304. When the first damping sealing sleeve 303-1 slides to the position of the air inlet 304-2, the air inlet 304-2 cooperates with the first damping sealing sleeve 303-1, and the air inlet 304-2 forms a sealed state.
[0035] The front end of the sleeve 304 is provided with a second damping sealing sleeve 304-3, which is slidably connected to the rod body of the ear rod 305 and can prevent the limit frame at the end of the ear rod 305 from passing through, thereby limiting the displacement length of the ear rod 305. The rear end of the ear rod 305 is provided with an airbag inflation port 305-2. When the ear rod 305 is displaced to the farthest distance, the airbag inflation port 305-2 is located at the position corresponding to the second damping sealing sleeve 304-3.
[0036] When the hand wheel 302 is turned, the screw rod 301 pushes the pull rod 303 forward, driving the ear rod 305 and the first damping sealing sleeve 303-1 to move forward. When the first damping sealing sleeve 303-1 exceeds the air inlet 304-2, a closed chamber is formed on the right side of the first damping sealing sleeve 303-1, that is, the space formed by the first damping sealing sleeve 303-1, the second damping sealing sleeve 304-3 and the ear rod 305; as the push continues, the gas accommodating space becomes smaller, and the gas enters the ear rod 305 through the airbag inflation port 305-2 to expand the airbag 305-1. The airbag 305-1 is set to a certain shape and pressure. When a certain pressure is reached, the gas will overflow from the pressure relief port 304-1 to prevent the airbag 305-1 from exceeding the set expansion range.
[0037] After the experiment is completed, the hand wheel 302 is reversed and the air bag 305-1 is contracted, which makes it easy to take out the ear rod 305. After exceeding the air inlet 304-2, the device returns to the initial state, which greatly improves the effect and efficiency of fixing the ear rod 305. Embodiment 2
[0038] refer to Figure 11-Figure 18 A tail-flicking anesthesia supplementation mechanism 5 is provided, which automatically supplements anesthesia according to the mouse's instinctive tail-flicking reaction, cooperates with manual anesthesia, reduces personnel energy consumption, and improves anesthesia degree control.
[0039] The operating base 1 is also provided with a second mounting seat 104, which is arranged in front of the placement platform 101, that is, in front of the mouse head. The second mounting seat 104 is provided with a mask 4, which is connected to the second mounting seat 104 by an elastic and bendable fixing rod 401, and the other end of the fixing rod 401 is connected to a connecting pipe 402, and the connecting pipe 402 is connected to a trumpet-shaped accommodation pipe 403, and the head of the mouse can be placed in the accommodation pipe 403. The accommodation pipe 403 is made of elastic rubber material, which has a certain sealing and tightening opening function, so that the mouse head fits the accommodation pipe 403.
[0040] The lower part of the connecting tube 402 is connected to an anesthesia chamber 509 through a hose, and the anesthesia chamber 509 is arranged on the operating base 1, and is located between the mounting seat 2 104 and the placing table 101. An opening and a connecting cover are arranged on the anesthesia chamber 509. A one-way valve is arranged in the hose.
[0041] A vent 404 is provided on the side of the connecting tube 402, which can play the role of anesthesia neutralization and filtration. A one-way valve is provided at the end of the vent 404, which allows air to pass through to ensure the breathing of the mouse. A structure for neutralizing and filtering anesthetic gas is also provided in the vent 404. After the experiment, the anesthetic gas entering the vent 404 can be processed to lose its anesthetic effect, thereby preventing the anesthetic gas from overflowing and causing harm to the experimenter. This is not an improvement point here, and the anesthetic gas processing device in the prior art is adopted, so it will not be described in detail.
[0042] The operating base 1 is also provided with a mounting seat 3 105, and a rotating seat 501 is provided on one side of the mounting seat 3 105 close to the mounting seat 2 104. The rotating seat 501 is rotatably connected to a rotating rod 502, and an elastic rope 503 is fixedly connected below the rotating rod 502. The other end of the elastic rope 503 is fixedly connected to a tail clamp 504, and the tail clamp 504 includes a receiving tube 403 and operating blocks on both sides. The tail clamp 504 adopts an elastic opening design, and the operating blocks on both sides are opened, and the tail is placed in the receiving tube 403. After being released, the receiving tube 403 continuously applies a set extrusion force to the tail. The end of the tail clamp 504 is connected to the elastic rope 503, and when the tail is swung, it can adapt to the pulling at any angle and direction.
[0043] The design of the rotating rod 502 utilizes the principle of lever. The magnitude of the torque multiplied by the force at both ends of the lever is the same. Therefore, the rotating seat 501 is located near the top of the rotating rod 502. The lower end of the rotating rod 502 is pulled by the elastic rope 503, and the torque is large, which makes the torque at the top smaller, thereby preliminarily amplifying the pulling force.
[0044] The mounting seat 3 105 is provided with a receiving hole, in which a first spring piston rod 505 is arranged, and the first spring piston rod 505 comprises a spring 1 505-1, a pressing rod 505-2, a pressing cap 505-3, and a pushing plate 505-4, wherein the pressing rod 505-2, the pressing cap 505-3, and the pushing plate 505-4 are integrally connected, and the spring 1 505-1 is sleeved on the pressing rod 505-2, one end of which is connected to the pressing cap 505-3, and the other end of which is connected to the side wall of the mounting seat 3 105 close to the mounting seat 2 104. The upward end of the rotating rod 502 contacts the pressing cap 505-3 of the mounting seat 3 105.
[0045] A hydraulic pipe 506 is also provided in the receiving hole, and a pressing rod 505-2 of the first spring piston rod 505 extends into the hydraulic pipe 506. The hydraulic pipe 506 is arranged along the operating base 1, and the other end is connected to a hydraulic amplification chamber 507, which is arranged on the operating base 1, and has hydraulic grooves 507-1 with different diameters arranged inside. The hydraulic pipe 506 is connected to the hydraulic groove 1 with a smaller diameter, and a second spring piston rod 508 is arranged in the hydraulic groove 2 with a larger diameter. The pressing cap 505-3 is located in the hydraulic groove 2, and the pushing disk 505-4 is located in the air inlet pipe 509-1 of the anesthesia chamber 509. The second spring sleeve is arranged on the pressing rod 505-2, one end of which is connected to the pressing cap 505-3, and the other end is connected to the air inlet end of the air inlet pipe 509-1.
[0046] The hydraulic amplification chamber 507 utilizes the cross-sectional change of the hydraulic groove 507-1 and applies the Pascal principle. The pressure of the liquid in the hydraulic groove 507-1 is the same. As the cross-sectional area increases from the hydraulic groove 1 to the hydraulic groove 2, the pressure increases. A common example of using this principle is a hydraulic jack.
[0047] The end of the tail clamp 504 is connected to an elastic rope 503. When the tail is swung, it can adapt to pulling at any angle and direction. The elastic rope 503 drives the rotating rod 502 to rotate, and the pulling force of the elastic rope 503 is initially amplified to squeeze the first spring piston rod 505, and squeeze the hydraulic pipe 506 fixed on the mounting seat 3 105, so as to convert the amplified pulling force into liquid pressure and transmit it to the hydraulic amplification chamber 507. The force amplified by the hydraulic amplification chamber 507 for the second time pushes the second spring piston rod 508 to move, squeeze the anesthetic gas chamber, and make the anesthetic gas enter the mask 4 through the hose, thereby realizing automatic anesthesia replenishment.
[0048] Application Cases
[0049] The use process of the present invention is: 1. Preparation: Prepare the necessary tools and materials, including mice, ear bars 305 (usually made of metal), anesthetics (such as isoflurane), and disinfectant.
[0050] 2. Mouse anesthesia: Place the mouse in an anesthesia box and anesthetize it with anesthetic.
[0051] Wait until the mouse is fully anesthetized, with steady breathing and sluggish corneal reflexes.
[0052] 3. Preparation of surgical area: The mouse is taken out of the anesthesia box and placed on the placement table 101 . The mouse's limbs are spread out on the placement table 101 , and the mouse's head is placed in the accommodation tube 403 .
[0053] Wipe the mouse's head with disinfectant solution to ensure cleanliness and sterility of the surgical area.
[0054] 4. Fixing of ear rod 305: The hand wheel 302 rotates to drive the screw rod 301 to move forward, thereby inflating the airbag 305-1 and expanding the airbag 305-1, thereby increasing the adaptability of contact with the mouse ear and ensuring that the degree of clamping is moderate, so as not to damage the mouse ear and not to cause the mouse head to shake due to being too loose. The head posture of the mouse is adjusted so that its head is in a straight line with the body to ensure the accuracy of the experiment.
[0055] 6. Comfortability ensured: Check the comfort of the mouse regularly to ensure that the fixation of the ear bars 305 does not cause it unnecessary pain.
[0056] 7. Supplementary anesthesia: The operating blocks on both sides of the tail clamp 504 are opened, and the tail is placed in the accommodating tube 403. After the clamp is released, the accommodating tube 403 continues to apply a set squeezing force to the tail. At this time, the mouse tail is in a stretched state; when the anesthetic effect of the mouse weakens during the experiment, the mouse will have an instinctive physical reaction. At this time, the stimulation of the tail by the tail clamp 504 will cause the mouse tail to swing. The end of the tail clamp 504 is connected to an elastic rope 503. When the tail swings, it can adapt to pulling at any angle and direction. The elastic rope 503 drives the rotating rod 502 to rotate, and the pulling force of the elastic rope 503 is initially amplified to squeeze the first spring piston rod 505, squeeze the hydraulic pipe 506 fixed on the mounting seat 3 105, and convert the amplified pulling force into liquid pressure, which is transmitted to the hydraulic amplification chamber 507. The force amplified by the hydraulic amplification chamber 507 for the second time pushes the second spring piston rod 508 to move, squeeze the anesthetic gas chamber, and allow the anesthetic gas to enter the mask 4 through the hose.
[0057] The above shows and describes the basic principles, main features and advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments, and the above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.
Claims
1. A modeling device for fixing the head of a newborn mouse and performing supplementary anesthesia, comprising an operating base (1), characterized in that: Also includes: The ear rod assembly (3) is provided in two groups and is symmetrically distributed on both sides of the mouse fixed on the operating base (1); The tail-flicking supplementary anesthesia mechanism (5) comprises a supplementary anesthesia component and a detection and transmission component, wherein the supplementary anesthesia component and the detection and transmission component are arranged on an operating base (1); the detection and transmission component clamps the tail of a mouse and triggers the supplementary anesthesia component to supplement anesthesia of the mouse by detecting the mouse's tail flicking.
2. A modeling device for fixing the head of a newborn mouse and performing supplementary anesthesia according to claim 1, characterized in that: The detection transmission assembly comprises a tail clamp (504), the tail clamp (504) is connected to an elastic rope (503), and the elastic rope (503) is connected to a first amplification unit.
3. A modeling device for fixing the head of a newborn mouse and performing supplementary anesthesia according to claim 2, characterized in that: The first amplifying unit comprises a mounting seat three (105) arranged on the operating base (1), the mounting seat three (105) being provided with a rotating seat (501), the rotating seat (501) being rotatably connected to a rotating rod (502), and the rotating rod (502) being connected to an elastic rope (503).
4. A modeling device for fixing the head of a newborn mouse and performing supplementary anesthesia according to claim 3, characterized in that: A first spring piston rod (505) is also provided on the mounting seat three (105), the first spring piston rod (505) comprising a spring one (505-1) and a piston rod, the spring one (505-1) is sleeved on the piston rod, the spring one (505-1) is connected to the mounting seat three (105), and the rotating rod (502) is in contact with the piston rod.
5. A modeling device for fixing the head of a newborn mouse and performing supplementary anesthesia according to claim 4, characterized in that: The first spring piston rod (505) is connected to a hydraulic pipe (506), the other end of the hydraulic pipe (506) is connected to a second amplification unit, the second amplification unit comprises a hydraulic amplification chamber (507), hydraulic grooves (507-1) of different diameters are arranged in the hydraulic amplification chamber (507), and the hydraulic pipe (506) is connected to the hydraulic groove (507-1) of the smaller diameter.
6. A modeling device for fixing the head of a newborn mouse and performing supplementary anesthesia according to claim 5, characterized in that: The supplementary anesthesia component comprises an anesthesia chamber (509), and the anesthesia chamber (509) is connected to the hydraulic amplification chamber (507) via a second spring piston rod (508).
7. A modeling device for fixing the head of a newborn mouse and performing supplementary anesthesia according to claim 1, characterized in that: The ear rod assembly (3) comprises a pull rod (303), a sleeve (304) is sleeved on the pull rod (303), the sleeve (304) is slidably connected to the pull rod (303), one end of the pull rod (303) is connected to the ear rod (305), and the other end of the pull rod (303) is movably connected to the screw rod (301).
8. A modeling device for fixing the head of a newborn mouse and performing supplementary anesthesia according to claim 7, characterized in that: The sleeve (304) is provided with an air inlet (304-2), the ear rod (305) is provided with an airbag inflation port (305-2), and the front end of the ear rod (305) is provided with an airbag (305-1).
9. A method for using a modeling device for fixing the head of a newborn mouse and performing supplementary anesthesia, characterized in that: Using the modeling device for fixing the head of a newborn mouse and performing supplementary anesthesia as described in any one of claims 1 to 8, the specific method is as follows: S1, performing preliminary anesthesia on the mouse, rotating the screw rod (301) to make it move forward, and at the same time inflating the airbag (305-1), expanding the airbag (305-1), and fixing the head of the mouse; S2, opening the tail clamp (504), placing the mouse tail in it, and clamping the mouse tail; S3. When the anesthetic effect of the mouse weakens during the experiment, the mouse body reacts instinctively to cause the mouse to flick its tail. When the detection and transmission component detects the mouse flicking its tail, the supplementary anesthesia component provides supplementary anesthesia to the mouse.
10. The method for using the modeling device for fixing the head of a newborn mouse and performing supplementary anesthesia according to claim 9, characterized in that: Among them, S3 is specifically: When the mouse's tail swings, the tail clamp (504) pulls the elastic rope (503), and the elastic rope (503) drives the rotating rod (502) to rotate, and the pulling force of the elastic rope (503) is initially amplified to squeeze the first spring piston rod (505), and then squeeze the hydraulic pipe (506), and the amplified pulling force is converted into liquid pressure, which is transmitted to the hydraulic amplification chamber (507) through the hydraulic pipe (506). The force amplified for the second time by the hydraulic amplification chamber (507) pushes the second spring piston rod (508) to move, squeezes the anesthetic gas chamber, and supplements anesthesia for the mouse.