Method for extracting multiple components from herba epimedii

Abstract

The invention discloses a method for extracting various components from herba epimedii, which comprises the following steps: A, firstly, cleaning stems and leaves of herba epimedii serving as raw materials, and drying after cleaning; and B, crushing and grinding the cleaned and dried stems and leaves of the herba epimedii into fine powder so as to improve the extraction efficiency. According to the method, the herba epimedii raw material can be prepared into the flavonoid compound, the polysaccharide substance and the alkaloid through a preparation mode with high similarity, the preparation modes of the three substances are only slightly different, after one substance is prepared, the raw material can be reused, a corresponding solvent is selected for re-preparation, and the preparation process is simple and convenient. And proper paper cups are selected according to the types of the prepared materials, so that additional products are obtained, the utilization rate of the herba epimedii raw material can be increased, loss is avoided, more beneficial components can be obtained, and the use effect of the herba epimedii is improved.

Description

Technical Field

[0001] The invention relates to the technical field of drug extraction, in particular to a method for extracting multiple components from epimedium. Background Art

[0002] Epimedium, also known as Epimedium or Curculigo, is a common Chinese herbal medicine, traditionally used as a herbal medicine for strengthening the kidneys and strengthening the yang, strengthening the bones and muscles. Epimedium contains a variety of medicinal ingredients, such as flavonoids, polysaccharides and alkaloids. These ingredients work together to give epimedium a variety of pharmacological activities, making it widely used in traditional Chinese medicine to improve male sexual function and enhance bone health.

[0003] Flavonoid compounds such as icariin and icariin are the main active ingredients in epimedium. These compounds are believed to be beneficial for enhancing sexual function, promoting blood circulation, protecting cardiovascular health, etc. Polysaccharides have antioxidant and immunomodulatory effects. Alkaloids such as berberine have anti-inflammatory and antibacterial effects. In the prior art, the extraction of the heavy components of epimedium is mainly concentrated in flavonoid compounds, namely icariin and icariin, etc., so that other beneficial ingredients will be wasted with the extraction of flavonoid compounds, making the beneficial ingredients in epimedium unable to be utilized. Summary of the invention

[0004] The purpose of the present invention is to provide a method for extracting multiple components from epimedium, which has the advantage of full utilization and solves the problem that the extraction of heavy components of epimedium is mainly concentrated in flavonoid compounds, namely icariin, icariin, etc., so that other beneficial components are wasted along with the extraction of flavonoid compounds, and the beneficial components in epimedium cannot be utilized.

[0005] To achieve the above object, the present invention provides the following technical solution: a method for extracting multiple components from Epimedium, the extraction method comprising the following steps:

[0006] A. First, the stems and leaves of Epimedium used as raw materials are cleaned and dried after cleaning;

[0007] B. Then, the washed and dried epimedium stems and leaves are crushed and ground into fine powder to increase the extraction efficiency;

[0008] C. Selecting a suitable solvent according to the type of the target preparation, adding the epimedium powder and the selected solvent into a suitable container, performing extraction, and obtaining an extract;

[0009] D. filtering the extract to remove solid residues therein to obtain an extract;

[0010] E. Concentrating the extract by evaporating the solvent until the solution is concentrated to the desired concentration;

[0011] F. Choose the appropriate separation and precipitation method according to the type of target preparation:

[0012] f1: When the preparation is a flavonoid compound, an appropriate precipitant such as ether can be added to precipitate it. After precipitation, ethanol is used for washing, purification and collection;

[0013] f2: When the preparation is a polysaccharide, the polysaccharide can be precipitated by adding an appropriate precipitant such as ethanol. After precipitation, the polysaccharide is washed with appropriate ether and collected;

[0014] f3: When the preparation is an alkaloid, the alkaloid compound can be further separated and purified by chromatography to remove impurities, obtain a higher purity compound and collect it;

[0015] H. Dry the collected preparation to obtain a solid product.

[0016] Preferably, in step A, drying can be performed in a drying chamber at a drying temperature between 40° C. and 50° C. This temperature range is relatively low and helps to retain the active ingredients in the herbs while effectively removing moisture.

[0017] Preferably, in step B, a grinder is used for crushing, and the particle size of the epimedium stems and leaves is controlled to be between 3 and 5 mm to ensure that the medicinal materials are fully exposed to the solvent. The ball mill model can be Retsch PM200.

[0018] Preferably, in step C, when the target preparation is flavonoids and alkaloids, ethanol is selected as the solvent, which can effectively extract a variety of natural products; when the target preparation is a polysaccharide, water is selected as the solvent, which is a commonly used solvent for extracting polysaccharides, because polysaccharides are generally soluble in water and can be extracted at room temperature, and the time is generally 10 to 24 hours.

[0019] Preferably, in step C, the extract may be heated during the extraction process, and the heating temperature is maintained in the range of 50° C. to 70° C. This temperature range is suitable for the dissolution and release of active ingredients in the epimedium, while avoiding the degradation of some ingredients due to excessively high temperatures.

[0020] Preferably, in step D, when the extract is less, the filtering method is filter paper filtration, and when the extract is more, a vacuum funnel filter can be used to improve efficiency.

[0021] Preferably, in step E, a rotary evaporator can be used to speed up the process. The rotary evaporator model is Buchi R-210, and the rotation speed is between 20 and 300 rpm, and increases with time.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] The present invention can prepare the raw material of epimedium into flavonoid compounds, polysaccharides and alkaloids through a preparation method with high similarity. The preparation methods of the three substances are only slightly different. After the preparation of one of the substances is completed, the raw material can be reused, and a corresponding solvent is selected for re-preparation. According to the type of the prepared product, a suitable paper cup basis is selected to obtain another product. Not only can the utilization rate of the raw material of epimedium be improved and the loss be avoided, but more beneficial components can also be obtained, and the use effect thereof is increased. The invention has the advantage of full utilization, and solves the problem that the extraction of the heavy components of epimedium is mainly concentrated in flavonoid compounds, namely icariin, icariin, etc., so that other beneficial components are wasted along with the extraction of flavonoid compounds, and the beneficial components in epimedium cannot be utilized.

[0024] Figure 1 It is a schematic diagram of the preparation process of the present invention. DETAILED DESCRIPTION

[0025] A method for extracting multiple components from Epimedium, the extraction method comprising the following steps:

[0026] A. First, the stems and leaves of Epimedium used as raw materials are cleaned and dried after cleaning;

[0027] B. Then, the washed and dried epimedium stems and leaves are crushed and ground into fine powder to increase the extraction efficiency;

[0028] C. Selecting a suitable solvent according to the type of the target preparation, adding the epimedium powder and the selected solvent into a suitable container, performing extraction, and obtaining an extract;

[0029] D. filtering the extract to remove solid residues therein to obtain an extract;

[0030] E. Concentrating the extract by evaporating the solvent until the solution is concentrated to the desired concentration;

[0031] F. Choose the appropriate separation and precipitation method according to the type of target preparation:

[0032] f1: When the preparation is a flavonoid compound, an appropriate precipitant such as ether can be added to precipitate it. After precipitation, ethanol is used for washing, purification and collection;

[0033] f2: When the preparation is a polysaccharide, the polysaccharide can be precipitated by adding an appropriate precipitant such as ethanol. After precipitation, the polysaccharide is washed with appropriate ether and collected;

[0034] f3: When the preparation is an alkaloid, the alkaloid compound can be further separated and purified by chromatography to remove impurities, obtain a higher purity compound and collect it;

[0035] G. Dry the collected preparation to obtain a solid product.

[0036] Embodiment 2:

[0037] In the first embodiment, the following steps are added:

[0038] In step A, drying can be performed in a drying room at a temperature between 40° C. and 50° C. This relatively low temperature range helps to retain the active ingredients in the herbs while effectively removing moisture.

[0039] The extraction method comprises the following steps:

[0040] A. First, the stems and leaves of Epimedium used as raw materials are cleaned and dried after cleaning;

[0041] B. Then, the washed and dried epimedium stems and leaves are crushed and ground into fine powder to increase the extraction efficiency;

[0042] C. Selecting a suitable solvent according to the type of the target preparation, adding the epimedium powder and the selected solvent into a suitable container, performing extraction, and obtaining an extract;

[0043] D. filtering the extract to remove solid residues therein to obtain an extract;

[0044] E. Concentrating the extract by evaporating the solvent until the solution is concentrated to the desired concentration;

[0045] F. Choose the appropriate separation and precipitation method according to the type of target preparation:

[0046] f1: When the preparation is a flavonoid compound, an appropriate precipitant such as ether can be added to precipitate it. After precipitation, ethanol is used for washing, purification and collection;

[0047] f2: When the preparation is a polysaccharide, the polysaccharide can be precipitated by adding an appropriate precipitant such as ethanol. After precipitation, the polysaccharide is washed with appropriate ether and collected;

[0048] f3: When the preparation is an alkaloid, the alkaloid compound can be further separated and purified by chromatography to remove impurities, obtain a higher purity compound and collect it;

[0049] The collected preparation was dried to obtain a solid product.

[0050] Embodiment three:

[0051] In the second embodiment, the following steps are added:

[0052] In step B, a grinder is used for crushing, and the particle size of the epimedium stems and leaves is controlled to be between 3 and 5 mm to ensure that the medicinal materials are fully exposed to the solvent. The ball mill model can be Retsch PM200.

[0053] The extraction method comprises the following steps:

[0054] A. First, the stems and leaves of Epimedium used as raw materials are cleaned and dried after cleaning;

[0055] B. Then, the washed and dried epimedium stems and leaves are crushed and ground into fine powder to increase the extraction efficiency;

[0056] C. Selecting a suitable solvent according to the type of the target preparation, adding the epimedium powder and the selected solvent into a suitable container, performing extraction, and obtaining an extract;

[0057] D. filtering the extract to remove solid residues therein to obtain an extract;

[0058] E. Concentrating the extract by evaporating the solvent until the solution is concentrated to the desired concentration;

[0059] F. Choose the appropriate separation and precipitation method according to the type of target preparation:

[0060] f1: When the preparation is a flavonoid compound, an appropriate precipitant such as ether can be added to precipitate it. After precipitation, ethanol is used for washing, purification and collection;

[0061] f2: When the preparation is a polysaccharide, the polysaccharide can be precipitated by adding an appropriate precipitant such as ethanol. After precipitation, the polysaccharide is washed with appropriate ether and collected;

[0062] f3: When the preparation is an alkaloid, the alkaloid compound can be further separated and purified by chromatography to remove impurities, obtain a higher purity compound and collect it;

[0063] G. Dry the collected preparation to obtain a solid product.

[0064] Embodiment 43:

[0065] In the third embodiment, the following steps are added:

[0066] In step C, when the target preparation is flavonoids and alkaloids, ethanol is selected as the solvent, which can effectively extract a variety of natural products; when the target preparation is a polysaccharide, water is selected as the solvent, which is a commonly used solvent for extracting polysaccharides, because polysaccharides are generally soluble in water and can be extracted at room temperature, and the time is generally 10 to 24 hours.

[0067] The extraction method comprises the following steps:

[0068] A. First, the stems and leaves of Epimedium used as raw materials are cleaned and dried after cleaning;

[0069] B. Then, the washed and dried epimedium stems and leaves are crushed and ground into fine powder to increase the extraction efficiency;

[0070] C. Selecting a suitable solvent according to the type of the target preparation, adding the epimedium powder and the selected solvent into a suitable container, performing extraction, and obtaining an extract;

[0071] D. filtering the extract to remove solid residues therein to obtain an extract;

[0072] E. Concentrating the extract by evaporating the solvent until the solution is concentrated to the desired concentration;

[0073] F. Choose the appropriate separation and precipitation method according to the type of target preparation:

[0074] f1: When the preparation is a flavonoid compound, an appropriate precipitant such as ether can be added to precipitate it. After precipitation, ethanol is used for washing, purification and collection;

[0075] f2: When the preparation is a polysaccharide, the polysaccharide can be precipitated by adding an appropriate precipitant such as ethanol. After precipitation, the polysaccharide is washed with appropriate ether and collected;

[0076] f3: When the preparation is an alkaloid, the alkaloid compound can be further separated and purified by chromatography to remove impurities, obtain a higher purity compound and collect it;

[0077] G. Dry the collected preparation to obtain a solid product.

[0078] Embodiment five:

[0079] In the fourth embodiment, the following steps are added:

[0080] In the step C, the extract may be heated during the extraction process, and the heating temperature is maintained in the range of 50° C. to 70° C. This temperature range is suitable for the dissolution and release of active ingredients in the epimedium, while avoiding the degradation of some ingredients due to excessively high temperatures.

[0081] The extraction method comprises the following steps:

[0082] A. First, the stems and leaves of Epimedium used as raw materials are cleaned and dried after cleaning;

[0083] B. Then, the washed and dried epimedium stems and leaves are crushed and ground into fine powder to increase the extraction efficiency;

[0084] C. Selecting a suitable solvent according to the type of the target preparation, adding the epimedium powder and the selected solvent into a suitable container, performing extraction, and obtaining an extract;

[0085] D. filtering the extract to remove solid residues therein to obtain an extract;

[0086] E. Concentrating the extract by evaporating the solvent until the solution is concentrated to the desired concentration;

[0087] F. Choose the appropriate separation and precipitation method according to the type of target preparation:

[0088] f1: When the preparation is a flavonoid compound, an appropriate precipitant such as ether can be added to precipitate it. After precipitation, ethanol is used for washing, purification and collection;

[0089] f2: When the preparation is a polysaccharide, the polysaccharide can be precipitated by adding an appropriate precipitant such as ethanol. After precipitation, the polysaccharide is washed with appropriate ether and collected;

[0090] f3: When the preparation is an alkaloid, the alkaloid compound can be further separated and purified by chromatography to remove impurities, obtain a higher purity compound and collect it;

[0091] G. Dry the collected preparation to obtain a solid product.

[0092] Embodiment six:

[0093] In the fifth embodiment, the following steps are added:

[0094] In the step D, when the extract is less, the filtering method is filter paper filtering, and when the extract is more, a vacuum funnel filter can be used to improve efficiency.

[0095] The extraction method comprises the following steps:

[0096] A. First, the stems and leaves of Epimedium used as raw materials are cleaned and dried after cleaning;

[0097] B. Then, the washed and dried epimedium stems and leaves are crushed and ground into fine powder to increase the extraction efficiency;

[0098] C. Selecting a suitable solvent according to the type of the target preparation, adding the epimedium powder and the selected solvent into a suitable container, performing extraction, and obtaining an extract;

[0099] D. filtering the extract to remove solid residues therein to obtain an extract;

[0100] E. Concentrating the extract by evaporating the solvent until the solution is concentrated to the desired concentration;

[0101] F. Choose the appropriate separation and precipitation method according to the type of target preparation:

[0102] f1: When the preparation is a flavonoid compound, an appropriate precipitant such as ether can be added to precipitate it. After precipitation, ethanol is used for washing, purification and collection;

[0103] f2: When the preparation is a polysaccharide, the polysaccharide can be precipitated by adding an appropriate precipitant such as ethanol. After precipitation, the polysaccharide is washed with appropriate ether and collected;

[0104] f3: When the preparation is an alkaloid, the alkaloid compound can be further separated and purified by chromatography to remove impurities, obtain a higher purity compound and collect it;

[0105] G. Dry the collected preparation to obtain a solid product.

[0106] Embodiment seven:

[0107] In the sixth embodiment, the following steps are added:

[0108] In step E, a rotary evaporator can be used to speed up the process. The rotary evaporator model is Buchi R-210, and the rotation speed is between 20 and 300 rpm, and increases with time.

[0109] The extraction method comprises the following steps:

[0110] A. First, the stems and leaves of Epimedium used as raw materials are cleaned and dried after cleaning;

[0111] B. Then, the washed and dried epimedium stems and leaves are crushed and ground into fine powder to increase the extraction efficiency;

[0112] C. Selecting a suitable solvent according to the type of the target preparation, adding the epimedium powder and the selected solvent into a suitable container, performing extraction, and obtaining an extract;

[0113] D. filtering the extract to remove solid residues therein to obtain an extract;

[0114] E. Concentrating the extract by evaporating the solvent until the solution is concentrated to the desired concentration;

[0115] F. Choose the appropriate separation and precipitation method according to the type of target preparation:

[0116] f1: When the preparation is a flavonoid compound, an appropriate precipitant such as ether can be added to precipitate it. After precipitation, ethanol is used for washing, purification and collection;

[0117] f2: When the preparation is a polysaccharide, the polysaccharide can be precipitated by adding an appropriate precipitant such as ethanol. After precipitation, the polysaccharide is washed with appropriate ether and collected;

[0118] f3: When the preparation is an alkaloid, the alkaloid compound can be further separated and purified by chromatography to remove impurities, obtain a higher purity compound and collect it;

[0119] H. Dry the collected preparation to obtain a solid product.

[0120] The results of the embodiment are shown in the following table:

[0121]

[0122] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0123] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for extracting multiple components from Epimedium, characterized in that: The extraction method comprises the following steps: A. First, the stems and leaves of Epimedium used as raw materials are cleaned and dried after cleaning; B. Then, the washed and dried epimedium stems and leaves are crushed and ground into fine powder to increase the extraction efficiency; C. Selecting a suitable solvent according to the type of the target preparation, adding the epimedium powder and the selected solvent into a suitable container, performing extraction, and obtaining an extract; D. filtering the extract to remove solid residues therein to obtain an extract; E. Concentrating the extract by evaporating the solvent until the solution is concentrated to the desired concentration; F. Choose the appropriate separation and precipitation method according to the type of target preparation: f1: When the preparation is a flavonoid compound, an appropriate precipitant such as ether can be added to precipitate it. After precipitation, ethanol is used for washing, purification and collection; f2: When the preparation is a polysaccharide, the polysaccharide can be precipitated by adding an appropriate precipitant such as ethanol. After precipitation, the polysaccharide is washed with appropriate ether and collected; f3: When the preparation is an alkaloid, the alkaloid compound can be further separated and purified by chromatography to remove impurities, obtain a higher purity compound and collect it; G. Dry the collected preparation to obtain a solid product.

2. A method for extracting multiple components from Epimedium according to claim 1, characterized in that: In step A, drying can be performed in a drying room at a temperature between 40° C. and 50° C. This relatively low temperature range helps to retain the active ingredients in the herbs while effectively removing moisture.

3. The method for extracting multiple components from Epimedium according to claim 1, characterized in that: In step B, a grinder is used for crushing, and the particle size of the epimedium stems and leaves is controlled to be between 3 and 5 mm to ensure that the medicinal materials are fully exposed to the solvent. The ball mill model can be Retsch PM200.

4. The method for extracting multiple components from Epimedium according to claim 1, characterized in that: In step C, when the target preparation is flavonoids and alkaloids, ethanol is selected as the solvent, which can effectively extract a variety of natural products; when the target preparation is a polysaccharide, water is selected as the solvent, which is a commonly used solvent for extracting polysaccharides, because polysaccharides are generally soluble in water and can be extracted at room temperature, and the time is generally 10 to 24 hours.

5. The method for extracting multiple components from Epimedium according to claim 1, characterized in that: In the step C, the extract may be heated during the extraction process, and the heating temperature is maintained in the range of 50° C. to 70° C. This temperature range is suitable for the dissolution and release of active ingredients in the epimedium, while avoiding the degradation of some ingredients due to excessively high temperatures.

6. The method for extracting multiple components from Epimedium according to claim 1, characterized in that: In the step D, when the extract is less, the filtering method is filter paper filtering, and when the extract is more, a vacuum funnel filter can be used to improve efficiency.

7. The method for extracting multiple components from Epimedium according to claim 1, characterized in that: In step E, a rotary evaporator can be used to speed up the process. The rotary evaporator model is Buchi R-210, and the rotation speed is between 20 and 300 rpm, and increases with time.

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