Water-based bionic lubricant with better lubrication degree and durability and preparation method of water-based bionic lubricant

Abstract

The invention discloses a water-based bionic lubricant with better lubrication degree and durability and a preparation method thereof, the water-based bionic lubricant comprises the following components: a hyaluronic acid grafted glucosamine composition, hyaluronic acid, polyethylene glycol, beta-(1, 3)-glucan and water, the molecular weight of the hyaluronic acid is 60 kDa to 80 kDa, the molecular weight of the polyethylene glycol is 60 kDa to 80 kDa, and the molecular weight of the beta-(1, 3)-glucan is 50 kDa to 80 kDa. The molecular weight of the polyethylene glycol is 4000 to 6000. According to the invention, the formula of the lubricant is scientifically and reasonably designed, hyaluronic acid and polyethylene glycol with specific molecular weights are selected, and meanwhile, part of hyaluronic acid is grafted with glucosamine to prepare the hyaluronic acid grafted glucosamine composition; according to the hyaluronic acid grafted glucosamine composition, hyaluronic acid and other substances form a water-based lubricant which is reasonable in liquidity and good in lubricating performance, and especially in the aspects of durability and stability, the lubricant has outstanding performance and can be used for sexual life or medical examination of people.

Description

Technical Field

[0001] The invention relates to the technical field of medical device lubricants, and in particular to a water-based bionic lubricant with better lubricity and durability and a preparation method thereof. Background Art

[0002] The vagina secretes a clear liquid that acts as a natural lubricant, but there are many factors that affect its secretion, including estrogen levels, medications, or certain diseases (such as diabetes). Lubricants are often used in clinical medical examinations and during sexual intercourse to lubricate the drier vaginal opening and vaginal wall, avoiding unnecessary tissue trauma and improving the quality of sexual life.

[0003] Different from the lubricants used in machinery, human lubricants need to have wide applicability and high safety. Currently, there are mainly the following lubricants on the market, each with its own advantages and disadvantages.

[0004] Water-based lubricants are one of the most common choices because they are compatible with most skin types and do not cause allergic reactions. They may also be compatible with sex with condoms. However, water-based lubricants may require frequent reapplication after use because they can run off easily.

[0005] Another commonly used type is silicone-based lubricant: Silicone-based lubricants are more durable, so they usually do not need to be reapplied frequently, but they are not easy to clean, and residues left on the vaginal wall can easily induce gynecological inflammation.

[0006] There is also an oil-based lubricant, which has a good lubricating effect and lasts longer, but like petroleum lubricants, it dissolves latex and is not suitable for use with condoms because it may damage rubber condoms. At the same time, similar to silicone-based lubricants, oil-based lubricants are not easy to clean in the vagina and may induce gynecological inflammation.

[0007] Therefore, developing an aqueous lubricant that is good in durability, lubricity, durability and easy to clean and suitable for use in condoms is an urgent problem to be solved by those skilled in the art. Summary of the invention

[0008] The purpose of the present invention is to provide a water-based bionic lubricant with better lubricity and durability, which can be used in daily life or clinical medical examinations and has better lubricity and durability.

[0009] Furthermore, the present invention also provides a method for preparing the above-mentioned aqueous bionic lubricant with better lubricity and durability.

[0010] The technical solution adopted by the present invention is: A water-based bionic lubricant with better lubricity and durability comprises the following components by weight percentage: Hyaluronic acid grafted glucosamine composition 2.0%-2.5%; Hyaluronic acid 2.5%-3.0%; Polyethylene glycol 1.2%-1.5%; β-(1,3)-glucan 2.0%-3.0%; Replenish water to 100%; Wherein, the hyaluronic acid-grafted glucosamine composition is prepared by the following method: 12.9-14.3 parts by weight of a condensing agent, 25.7-30 parts by weight of glucosamine and 200 parts by weight of water are uniformly mixed, sodium bicarbonate is added to adjust the pH to 7.5, and then 57.1-60.7 parts by weight of hyaluronic acid is added dropwise while stirring for 30 minutes, citric acid is added to adjust the pH to 6.5, the reaction is continued for 2 hours, and then dehydrated and dried to obtain a hyaluronic acid-grafted glucosamine composition; The molecular weight of the hyaluronic acid is 60kDa-80kDa; The molecular weight of the polyethylene glycol is 4000-6000.

[0011] Preferably, the aqueous biomimetic lubricant with better lubricity and durability of the present invention comprises the following components by weight percentage: Hyaluronic acid grafted glucosamine composition 2.2%; Hyaluronic acid 2.7%; Polyethylene glycol 1.4%; β-(1,3)-glucan 2.5%; Water 91.2%.

[0012] Wherein, the condensing agent is selected from one or more of dicyclohexylcarbodiimide, diisopropylcarbodiimide and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide.

[0013] Wherein, the hyaluronic acid-grafted glucosamine composition is prepared by the following method: 14.3 parts by weight of dicyclohexylcarbodiimide, 27.3 parts by weight of glucosamine and 200 parts by weight of water are uniformly mixed, sodium bicarbonate is added to adjust the pH to 7.5, and then 57.1-60.7 parts by weight of hyaluronic acid are added dropwise while stirring, and the addition is completed within 30 minutes. Citric acid is added to adjust the pH to 6.5, and the reaction is continued for 2 hours, followed by dehydration and drying to obtain a hyaluronic acid-grafted glucosamine composition.

[0014] Wherein, the hyaluronic acid-grafted glucosamine composition is prepared by the following method: 14.3 parts by weight of dicyclohexylcarbodiimide, 27.3 parts by weight of glucosamine and 200 parts by weight of water were uniformly mixed, sodium bicarbonate was added to adjust the pH to 7.5, and then 58.4 parts by weight of hyaluronic acid was added dropwise while stirring. The addition took 30 minutes, and citric acid was added to adjust the pH to 6.5. The reaction was continued for 2 hours, and then dehydrated and dried to obtain a hyaluronic acid-grafted glucosamine composition.

[0015] A method for preparing the above-mentioned water-based biomimetic lubricant with better lubricity and durability comprises the following steps: (1) Prepare materials in proportion; (2) dissolving the hyaluronic acid-grafted glucosamine composition in water at room temperature to prepare an aqueous solution of the hyaluronic acid-grafted glucosamine composition, then adding hyaluronic acid and stirring evenly to obtain a solution A; (3) Under stirring conditions, dissolving β-(1,3)-glucan in water to prepare a β-(1,3)-glucan aqueous solution to obtain solution B; (4) Mixing polyethylene glycol and the remaining water uniformly to obtain a mixed solution C; (5) Under stirring conditions, solution A is slowly poured into mixed solution C. After stirring for 30 minutes, solution B is slowly poured into the mixed solution under continued stirring to obtain a transparent solution, which is the water-based bionic lubricant.

[0016] The step (2) specifically comprises: dissolving the hyaluronic acid-grafted glucosamine composition in water at room temperature to prepare a 5 wt % aqueous solution of the hyaluronic acid-grafted glucosamine composition, then adding hyaluronic acid, and stirring evenly to obtain solution A.

[0017] Specifically, step (3) comprises: dissolving β-(1,3)-glucan in water under stirring conditions to prepare a 10 wt% β-(1,3)-glucan solution to obtain solution B.

[0018] Wherein, the stirring temperature of step (3) is 55°C-65°C.

[0019] Step (4) is specifically as follows: slowly pouring solution A into mixed solution C under stirring at 30°C-35°C, stirring for 30 minutes, and then slowly pouring solution B into the mixed solution under continued stirring to obtain a transparent solution, which is the water-based bionic lubricant.

[0020] In summary, the method for preparing a water-based bionic lubricant with better lubricity and durability according to the present invention comprises the following steps: (1) Prepare materials in proportion; (2) dissolving the hyaluronic acid-grafted glucosamine composition in water at room temperature to prepare a 5 wt % solution of the hyaluronic acid-grafted glucosamine composition, then adding hyaluronic acid and stirring to obtain a solution A; (3) Dissolving β-(1,3)-glucan in water at 55°C-65°C with stirring to prepare a 10 wt% β-(1,3)-glucan solution to obtain solution B; (4) Mixing polyethylene glycol and the remaining water uniformly to obtain a mixed solution C; (5) Under stirring conditions at 30°C-35°C, slowly pour solution A into mixed solution C. After stirring for 30 min, slowly pour solution B into the mixed solution under continued stirring to obtain a transparent solution, which is the water-based bionic lubricant.

[0021] It should be noted that the molecular weight referred to in the present invention refers to the average molecular weight.

[0022] Compared with the prior art, the aqueous biomimetic lubricant provided by the present invention has better lubricity and durability, and comprises the following components by weight percentage: 2.0%-2.5% hyaluronic acid grafted glucosamine composition; 2.5%-3.0% hyaluronic acid; 1.2%-1.5% polyethylene glycol; 2.0%-3.0% β-(1,3)-glucan; the balance is water; wherein the hyaluronic acid grafted glucosamine composition is prepared by the following method: 12.9-14.3 parts by weight of a condensing agent, 25.7-30 parts by weight of glucosamine and 200 parts by weight of water are mixed evenly, sodium bicarbonate is added to adjust the pH to 7.5, and then 57.1-60.7 parts by weight of hyaluronic acid are added dropwise while stirring, and the addition is completed in 30 minutes, citric acid is added to adjust the pH to 6.5, and the reaction is continued for 2 hours, and then dehydrated and dried to obtain a hyaluronic acid-grafted glucosamine composition; the molecular weight of the hyaluronic acid is 60kDa-80kDa; the molecular weight of the polyethylene glycol is 4000-6000. The present invention scientifically and rationally designs the formula of the lubricant, selects hyaluronic acid and polyethylene glycol of specific molecular weight, and at the same time, first grafts part of the hyaluronic acid with glucosamine to prepare a hyaluronic acid-grafted glucosamine composition, and the hyaluronic acid-grafted glucosamine composition, hyaluronic acid and the remaining substances form a water-based lubricant with reasonable fluidity and good lubrication performance, especially in terms of durability and stability. The lubricant of the present invention has outstanding performance and can be used in people's sexual life or medical examinations. DETAILED DESCRIPTION

[0023] In order to enable those skilled in the art to better understand the scheme of the present invention, the present invention is further described in detail below in conjunction with specific implementation methods. However, it should be noted that the embodiments of the present invention cannot be regarded as limiting the scope of the present invention.

[0024] Source of materials or preparation instructions: Hyaluronic acid a: Shanghai Yuchun Biotechnology Co., Ltd., molecular weight 10kDa; Hyaluronic acid b: Qingdao Keyes Biotechnology Co., Ltd., molecular weight 60 kDa; Hyaluronic acid c: Qingdao Keyes Biotechnology Co., Ltd., molecular weight 80 kDa; Hyaluronic acid d: Qingdao Keyes Biotechnology Co., Ltd., molecular weight 260 kDa; Polyethylene glycol: Hunan Huateng Pharmaceutical Co., Ltd., model HO002002-1.2K (molecular weight 1200), HO002002-4K (molecular weight 4000), HO002002-6K (molecular weight 6000), HO002002-10k (molecular weight 10000); The remaining substances are commercially available with a purity of more than 99%. Example 1

[0025] Preparation of hyaluronic acid grafted glucosamine composition: 14.3 parts by weight of dicyclohexylcarbodiimide, 27.3 parts by weight of glucosamine and 200 parts by weight of water were uniformly mixed, sodium bicarbonate was added to adjust the pH to 7.5, and then 58.4 parts by weight of hyaluronic acid b was added dropwise while stirring for 30 minutes, citric acid was added to adjust the pH to 6.5, the reaction was continued for 2 hours, and then dehydrated and dried to obtain a hyaluronic acid grafted glucosamine composition.

[0026] Then prepare the water-based lubricant as follows: (1) Prepare the materials according to the proportions shown in Table 1; (2) dissolving the hyaluronic acid-grafted glucosamine composition in water at room temperature to prepare a 5 wt % solution of the hyaluronic acid-grafted glucosamine composition, then adding hyaluronic acid b and stirring evenly to obtain solution A; (3) Dissolving β-(1,3)-glucan in water at 55°C-65°C with stirring to prepare a 10 wt% β-(1,3)-glucan solution to obtain solution B; (4) Mix polyethylene glycol (HO002002-4K) and the remaining water to obtain a mixed solution C; (5) Under stirring conditions at 30°C-35°C, slowly pour solution A into mixed solution C. After stirring for 30 min, slowly pour solution B into the mixed solution under continued stirring to obtain a transparent solution, which is the water-based bionic lubricant. Example 2

[0027] Preparation of hyaluronic acid grafted glucosamine composition: 13.6 parts by weight of dicyclohexylcarbodiimide, 25.7 parts by weight of glucosamine and 200 parts by weight of water were uniformly mixed, sodium bicarbonate was added to adjust the pH to 7.5, and then 60.7 parts by weight of hyaluronic acid c was added dropwise while stirring for 30 minutes, citric acid was added to adjust the pH to 6.5, the reaction was continued for 2 hours, and then dehydrated and dried to obtain a hyaluronic acid grafted glucosamine composition.

[0028] Then prepare the water-based lubricant as follows: (1) Prepare the materials according to the proportions shown in Table 1; (2) dissolving the hyaluronic acid-grafted glucosamine composition in water at room temperature to prepare a 5 wt % solution of the hyaluronic acid-grafted glucosamine composition, then adding hyaluronic acid C and stirring evenly to obtain solution A; (3) Dissolving β-(1,3)-glucan in water at 55°C-65°C with stirring to prepare a 10 wt% β-(1,3)-glucan solution to obtain solution B; (4) Mix polyethylene glycol (HO002002-6K) and the remaining water to obtain a mixed solution C; (5) Under stirring conditions at 30°C-35°C, slowly pour solution A into mixed solution C. After stirring for 30 min, slowly pour solution B into the mixed solution under continued stirring to obtain a transparent solution, which is the water-based bionic lubricant. Example 3

[0029] Preparation of hyaluronic acid grafted glucosamine composition: 12.9 parts by weight of dicyclohexylcarbodiimide, 30 parts by weight of glucosamine and 200 parts by weight of water were uniformly mixed, sodium bicarbonate was added to adjust the pH to 7.5, and then 57.1 parts by weight of hyaluronic acid b was added dropwise while stirring for 30 minutes, citric acid was added to adjust the pH to 6.5, the reaction was continued for 2 hours, and then dehydrated and dried to obtain a hyaluronic acid grafted glucosamine composition.

[0030] Then prepare the water-based lubricant as follows: (1) Prepare the materials according to the proportions shown in Table 1; (2) dissolving the hyaluronic acid-grafted glucosamine composition in water at room temperature to prepare a 5 wt % solution of the hyaluronic acid-grafted glucosamine composition, then adding hyaluronic acid b and stirring evenly to obtain solution A; (3) Dissolving β-(1,3)-glucan in water at 55°C-65°C with stirring to prepare a 10 wt% β-(1,3)-glucan solution to obtain solution B; (4) Mix polyethylene glycol (HO002002-4K) and the remaining water to obtain a mixed solution C; (5) Under stirring conditions at 30°C-35°C, slowly pour solution A into mixed solution C. After stirring for 30 min, slowly pour solution B into the mixed solution under continued stirring to obtain a transparent solution, which is the water-based bionic lubricant. Comparative Example 1

[0031] Prepare the water-based lubricant as follows: (1) Prepare the materials according to the proportions shown in Table 1; (2) at room temperature, dissolving hyaluronic acid b in water to prepare a 5 wt % solution of hyaluronic acid b, and stirring to obtain a solution A; (3) Dissolving β-(1,3)-glucan in water at 55°C-65°C with stirring to prepare a 10 wt% β-(1,3)-glucan solution to obtain solution B; (4) Mix polyethylene glycol (HO002002-4K) and the remaining water to obtain a mixed solution C; (5) Under stirring conditions at 30°C-35°C, slowly pour solution A into mixed solution C. After stirring for 30 min, slowly pour solution B into the mixed solution under continued stirring to obtain a transparent solution, which is the water-based bionic lubricant. Comparative Example 2

[0032] Preparation of hyaluronic acid grafted glucosamine composition: 14.3 parts by weight of dicyclohexylcarbodiimide, 27.3 parts by weight of glucosamine and 200 parts by weight of water were uniformly mixed, sodium bicarbonate was added to adjust the pH to 7.5, and then 58.4 parts by weight of hyaluronic acid b was added dropwise while stirring for 30 minutes, citric acid was added to adjust the pH to 6.5, the reaction was continued for 2 hours, and then dehydrated and dried to obtain a hyaluronic acid grafted glucosamine composition.

[0033] Then prepare the water-based lubricant as follows: (1) Prepare the materials according to the proportions shown in Table 1; (2) dissolving the hyaluronic acid-grafted glucosamine composition in water at room temperature to prepare a 5 wt % solution of the hyaluronic acid-grafted glucosamine composition, then adding hyaluronic acid b and stirring evenly to obtain solution A; (3) Mix polyethylene glycol (HO002002-4K) and the remaining water to obtain a mixed solution C; (5) Under stirring conditions at 30°C-35°C, slowly pour solution A into mixed solution C. After stirring for 30 minutes, a transparent solution is obtained, which is the water-based bionic lubricant. Comparative Example 3

[0034] Preparation of hyaluronic acid grafted glucosamine composition: 14.3 parts by weight of dicyclohexylcarbodiimide, 27.3 parts by weight of glucosamine and 200 parts by weight of water were uniformly mixed, sodium bicarbonate was added to adjust the pH to 7.5, and then 58.4 parts by weight of hyaluronic acid b was added dropwise while stirring for 30 minutes, citric acid was added to adjust the pH to 6.5, the reaction was continued for 2 hours, and then dehydrated and dried to obtain a hyaluronic acid grafted glucosamine composition.

[0035] Then prepare the water-based lubricant as follows: (1) Prepare the materials according to the proportions shown in Table 1; (2) dissolving the hyaluronic acid-grafted glucosamine composition in water at room temperature to prepare a 5 wt % solution of the hyaluronic acid-grafted glucosamine composition, then adding hyaluronic acid b and stirring evenly to obtain solution A; (3) Dissolving β-(1,3)-glucan in water at 55°C-65°C with stirring to prepare a 10 wt% β-(1,3)-glucan solution to obtain solution B; (4) Under stirring conditions at 30°C-35°C, slowly pour solution A into the remaining water. After stirring for 30 minutes, slowly pour solution B into the water while continuing to stir to obtain a transparent solution, which is the water-based bionic lubricant. Comparative Example 4

[0036] Preparation of hyaluronic acid grafted glucosamine composition: 14.3 parts by weight of dicyclohexylcarbodiimide, 27.3 parts by weight of glucosamine and 200 parts by weight of water were uniformly mixed, sodium bicarbonate was added to adjust the pH to 7.5, and then 58.4 parts by weight of hyaluronic acid b was added dropwise while stirring for 30 minutes, citric acid was added to adjust the pH to 6.5, the reaction was continued for 2 hours, and then dehydrated and dried to obtain a hyaluronic acid grafted glucosamine composition.

[0037] Then prepare the water-based lubricant as follows: (1) Prepare the materials according to the proportions shown in Table 1; (2) dissolving the hyaluronic acid-grafted glucosamine composition in water at room temperature to prepare a 5 wt % solution of the hyaluronic acid-grafted glucosamine composition, then adding hyaluronic acid b and stirring evenly to obtain solution A; (3) Dissolving β-(1,3)-glucan in water at 55°C-65°C with stirring to prepare a 10 wt% β-(1,3)-glucan solution to obtain solution B; (4) Mix polyethylene glycol (HO002002-10K) and the remaining water to obtain a mixed solution C; (5) Under stirring conditions at 30°C-35°C, slowly pour solution A into mixed solution C. After stirring for 30 min, slowly pour solution B into the mixed solution under continued stirring to obtain a transparent solution, which is the water-based bionic lubricant. Comparative Example 5

[0038] Preparation of hyaluronic acid grafted glucosamine composition: 14.3 parts by weight of dicyclohexylcarbodiimide, 27.3 parts by weight of glucosamine and 200 parts by weight of water were uniformly mixed, sodium bicarbonate was added to adjust the pH to 7.5, and then 58.4 parts by weight of hyaluronic acid b was added dropwise while stirring for 30 minutes, citric acid was added to adjust the pH to 6.5, the reaction was continued for 2 hours, and then dehydrated and dried to obtain a hyaluronic acid grafted glucosamine composition.

[0039] Then prepare the water-based lubricant as follows: (1) Prepare the materials according to the proportions shown in Table 1; (2) dissolving the hyaluronic acid-grafted glucosamine composition in water at room temperature to prepare a 5 wt % solution of the hyaluronic acid-grafted glucosamine composition, then adding hyaluronic acid b and stirring evenly to obtain solution A; (3) Dissolving β-(1,3)-glucan in water at 55°C-65°C with stirring to prepare a 10 wt% β-(1,3)-glucan solution to obtain solution B; (4) Mix polyethylene glycol (HO002002-1.2K) and the remaining water to obtain a mixed solution C; (5) Under stirring conditions at 30°C-35°C, slowly pour solution A into mixed solution C. After stirring for 30 min, slowly pour solution B into the mixed solution under continued stirring to obtain a transparent solution, which is the water-based bionic lubricant. Comparative Example 6

[0040] Preparation of hyaluronic acid grafted glucosamine composition: 14.3 parts by weight of dicyclohexylcarbodiimide, 27.3 parts by weight of glucosamine and 200 parts by weight of water were uniformly mixed, sodium bicarbonate was added to adjust the pH to 7.5, and then 58.4 parts by weight of hyaluronic acid d was added dropwise while stirring for 30 minutes, citric acid was added to adjust the pH to 6.5, the reaction was continued for 2 hours, and then dehydrated and dried to obtain a hyaluronic acid grafted glucosamine composition.

[0041] Then prepare the water-based lubricant as follows: (1) Prepare the materials according to the proportions shown in Table 1; (2) dissolving the hyaluronic acid-grafted glucosamine composition in water at room temperature to prepare a 5 wt % solution of the hyaluronic acid-grafted glucosamine composition, then adding hyaluronic acid d and stirring to obtain a solution A; (3) Dissolving β-(1,3)-glucan in water at 55°C-65°C with stirring to prepare a 10 wt% β-(1,3)-glucan solution to obtain solution B; (4) Mix polyethylene glycol (HO002002-4K) and the remaining water to obtain a mixed solution C; (5) Under stirring conditions at 30°C-35°C, slowly pour solution A into mixed solution C. After stirring for 30 min, slowly pour solution B into the mixed solution under continued stirring to obtain a transparent solution, which is the water-based bionic lubricant. Comparative Example 7

[0042] Preparation of hyaluronic acid grafted glucosamine composition: 14.3 parts by weight of dicyclohexylcarbodiimide, 27.3 parts by weight of glucosamine and 200 parts by weight of water were uniformly mixed, sodium bicarbonate was added to adjust the pH to 7.5, and then 58.4 parts by weight of hyaluronic acid a was added dropwise while stirring for 30 minutes, citric acid was added to adjust the pH to 6.5, the reaction was continued for 2 hours, and then dehydrated and dried to obtain a hyaluronic acid grafted glucosamine composition.

[0043] Then prepare the water-based lubricant as follows: (1) Prepare the materials according to the proportions shown in Table 1; (2) dissolving the hyaluronic acid-grafted glucosamine composition in water at room temperature to prepare a 5 wt % solution of the hyaluronic acid-grafted glucosamine composition, then adding hyaluronic acid a and stirring to obtain a solution A; (3) Dissolving β-(1,3)-glucan in water at 55°C-65°C with stirring to prepare a 10 wt% β-(1,3)-glucan solution to obtain solution B; (4) Mix polyethylene glycol (HO002002-4K) and the remaining water to obtain a mixed solution C; (5) Under stirring conditions at 30°C-35°C, slowly pour solution A into mixed solution C. After stirring for 30 min, slowly pour solution B into the mixed solution under continued stirring to obtain a transparent solution, which is the water-based bionic lubricant. Comparative Example 8

[0044] Preparation of hyaluronic acid grafted glucosamine composition: 14.3 parts by weight of dicyclohexylcarbodiimide, 40 parts by weight of glucosamine and 200 parts by weight of water were uniformly mixed, sodium bicarbonate was added to adjust the pH to 7.5, and then 45.7 parts by weight of hyaluronic acid b was added dropwise while stirring for 30 minutes, citric acid was added to adjust the pH to 6.5, the reaction was continued for 2 hours, and then dehydrated and dried to obtain a hyaluronic acid grafted glucosamine composition.

[0045] Then prepare the water-based lubricant as follows: (1) Prepare the materials according to the proportions shown in Table 1; (2) dissolving the hyaluronic acid-grafted glucosamine composition in water at room temperature to prepare a 5 wt % solution of the hyaluronic acid-grafted glucosamine composition, then adding hyaluronic acid b and stirring evenly to obtain solution A; (3) Dissolving β-(1,3)-glucan in water at 55°C-65°C with stirring to prepare a 10 wt% β-(1,3)-glucan solution to obtain solution B; (4) Mix polyethylene glycol (HO002002-4K) and the remaining water to obtain a mixed solution C; (5) Under stirring conditions at 30°C-35°C, slowly pour solution A into mixed solution C. After stirring for 30 min, slowly pour solution B into the mixed solution under continued stirring to obtain a transparent solution, which is the water-based bionic lubricant. Comparative Example 9

[0046] Preparation of hyaluronic acid grafted glucosamine composition: 14.3 parts by weight of dicyclohexylcarbodiimide, 20 parts by weight of glucosamine and 200 parts by weight of water were uniformly mixed, sodium bicarbonate was added to adjust the pH to 7.5, and then 65.7 parts by weight of hyaluronic acid b was added dropwise while stirring for 30 minutes, citric acid was added to adjust the pH to 6.5, the reaction was continued for 2 hours, and then dehydrated and dried to obtain a hyaluronic acid grafted glucosamine composition.

[0047] Then prepare the water-based lubricant as follows: (1) Prepare the materials according to the proportions shown in Table 1; (2) dissolving the hyaluronic acid-grafted glucosamine composition in water at room temperature to prepare a 5 wt % solution of the hyaluronic acid-grafted glucosamine composition, then adding hyaluronic acid b and stirring evenly to obtain solution A; (3) Dissolving β-(1,3)-glucan in water at 55°C-65°C with stirring to prepare a 10 wt% β-(1,3)-glucan solution to obtain solution B; (4) Mix polyethylene glycol (HO002002-4K) and the remaining water to obtain a mixed solution C; (5) Under stirring conditions at 30°C-35°C, slowly pour solution A into mixed solution C. After stirring for 30 min, slowly pour solution B into the mixed solution under continued stirring to obtain a transparent solution, which is the water-based bionic lubricant.

[0048] Detection Methods The biomimetic biolubricants prepared in the examples and comparative examples were tested according to the following method, and the results are listed in Table 2.

[0049] 1. Transmittance: Tested with a transmittance tester, the higher the transmittance, the better the transparency.

[0050] 2. pH value: The test is based on the relevant requirements for gel preparations in the Chinese Pharmacopoeia (2010).

[0051] 3. Fluidity: Conduct viscosity test on gel preparations according to the Chinese Pharmacopoeia (2010).

[0052] 4. Irritation: Apply the original solution evenly to the guinea pig's hair removal area, cover and fix with gauze, and continue for 6 days. Repeat on the 7th and 13th days to observe whether it causes sensitization.

[0053] 5. Microbial limit: The test is based on the relevant requirements for gel preparations in the Chinese Pharmacopoeia (2010).

[0054] 6. Lubrication performance (friction coefficient) The friction test was carried out on an improved MS-800 four-ball machine. Fresh dehaired pig skin was used as the lower test piece, and a Φ=25mm plastic sheet was used as the upper test piece for end face friction. The rotation speed was 100r.pm, and the loads were 5N, 10N, and 20N respectively. The friction coefficient was used to represent the lubrication performance. The smaller the friction coefficient, the better the lubrication performance.

[0055] 7. Stability test The samples were placed at -10°C for 48 hours, at 40°C for 48 hours, and at 45°C for 48 hours. The friction properties were then tested using the 6-friction test method and are listed in Table 2.

[0056] 8. Persistence Detection 10 parts by weight of the sample were mixed evenly with 1 part by weight of water, 0.02 parts by weight of pyridine, 0.02 parts by weight of squalene, 0.02 parts by weight of urea, 0.01 parts by weight of acetic acid, 0.03 parts by weight of lactic acid, and 0.02 parts by weight of propylene glycol, and then ground in an Anton Paar rabender rotary grinder at a speed of 100 r / min. After grinding for 3 minutes, the lubricating properties (friction coefficient) of the lubricant were tested and listed in Table 2. 1 part by weight of water, 0.02 parts by weight of pyridine, 0.02 parts by weight of squalene, 0.02 parts by weight of urea, 0.01 parts by weight of acetic acid, 0.03 parts by weight of lactic acid, and 0.02 parts by weight of propylene glycol are similar to vaginal secretions. After the sample was mixed and ground with similar vaginal secretions, its lubricating properties were tested, which can prove the durability of the sample.

[0057] Table 1 Ingredients of Examples and Comparative Examples (Unit: Weight Percentage)

[0058] Table 2 Comparison of lubricant performance between examples and comparative examples

[0059] It can be seen from the above data that when the viscosity of the lubricant is in the range of 80-90mPa.s, the friction coefficient is the smallest and the lubrication performance is the best.

[0060] As in Comparative Example 1, the hyaluronic acid-grafted glucosamine composition was not added to the system. Compared with Example 1, the friction coefficient of the lubricant was larger, and in the durability test, the friction coefficient increased.

[0061] In Comparative Example 2, β-(1,3)-glucan was not added to the system. Compared with Example 1, the friction coefficient of the lubricant was larger, and in the durability test, the friction coefficient increased.

[0062] In Comparative Example 3, polyethylene glycol is not added to the system. Polyethylene glycol can improve the stability of the lubricant system. Compared with Example 1, the friction coefficient of the lubricant in Comparative Example 3 is larger, and the friction coefficient increases in stability and durability tests.

[0063] In comparative example 4, the molecular weight of polyethylene glycol is too large. In the lubricant system, the large molecular weight of polyethylene glycol destroys the hydrogen bond connection force of hyaluronic acid and its grafts in the aqueous solution system, and the system is unstable. In the stability test, the friction coefficient increases significantly, indicating that its stability is poor.

[0064] In Comparative Example 5, the molecular weight of polyethylene glycol is too small, resulting in low viscosity and poor durability in the lubricant system.

[0065] Comparative Example 6: The molecular weight of hyaluronic acid is too large. In the lubricant system, the viscosity is too high and the friction is large. In Comparative Example 7, the molecular weight of hyaluronic acid is too small and the durability of the lubricant is poor.

[0066] In comparative example 8, the amount of glucosamine used in preparing the hyaluronic acid-grafted glucosamine composition is too much, and the hyaluronic acid-grafted glucosamine is uneven, resulting in the inability of the hyaluronic acid-grafted glucosamine and the hyaluronic acid to form a uniform hydrogen bond network in the aqueous solution system. The lubricant prepared with other substances has poor lubrication performance and poor durability.

[0067] In comparative example 9, the amount of glucosamine used in preparing the hyaluronic acid-grafted glucosamine composition is too small, and the hyaluronic acid-grafted glucosamine fails to form an effective graft, resulting in the inability of the hyaluronic acid-grafted glucosamine and the hyaluronic acid to form a better hydrogen bond network in the aqueous solution system. The lubricant prepared with other substances has poor lubrication performance and poor stability. After mixing with vaginal secretion-like fluid, the lasting performance is poor.

[0068] It can also be seen from the above data that the lubricating effect of the lubricant is affected by many factors. In the system of the present invention, the performance of the lubricant is related to the molecular weight of the main material hyaluronic acid and the molecular weight of polyethylene glycol. At the same time, whether part of the hyaluronic acid is grafted with glucosamine first also has a significant impact on the performance of the lubricant. The hyaluronic acid grafted glucosamine composition made from specific raw materials forms a more stable and slightly larger molecule, which can form a relatively stable hydrogen bond network with hyaluronic acid in water, and will not affect the fluidity of the lubricant. The formed lubricant has reasonable fluidity, which is convenient for production and use. It will also help to improve the durability of the lubricant, effectively solving the problem of poor durability of water-based lubricants.

[0069] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A water-based bionic lubricant with better lubricity and durability, characterized in that By weight percentage Includes the following ingredients: Hyaluronic acid grafted glucosamine composition 2.0%-2.5%; Hyaluronic acid 2.5%-3.0%; Polyethylene glycol 1.2%-1.5%; β-(1,3)-glucan 2.0%-3.0%; Replenish water to 100%; Wherein, the hyaluronic acid-grafted glucosamine composition is prepared by the following method: 12.9-14.3 parts by weight of a condensing agent, 25.7-30 parts by weight of glucosamine and 200 parts by weight of water are uniformly mixed, sodium bicarbonate is added to adjust the pH to 7.5, and then 57.1-60.7 parts by weight of hyaluronic acid is added dropwise while stirring for 30 minutes, citric acid is added to adjust the pH to 6.5, the reaction is continued for 2 hours, and then dehydrated and dried to obtain a hyaluronic acid-grafted glucosamine composition; The molecular weight of the hyaluronic acid is 60kDa-80kDa; The molecular weight of the polyethylene glycol is 4000-6000.

2. The water-based bionic lubricant with better lubricity and durability as claimed in claim 1, characterized in that: The following components are included by weight percentage: Hyaluronic acid grafted glucosamine composition 2.2%; Hyaluronic acid 2.7%; Polyethylene glycol 1.4%; β-(1,3)-glucan 2.5%; Water 91.2%.

3. The water-based bionic lubricant with better lubricity and durability as claimed in claim 1, characterized in that: The condensing agent is selected from one or more of dicyclohexylcarbodiimide, diisopropylcarbodiimide and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide.

4. The aqueous biomimetic lubricant with better lubricity and durability as claimed in claim 1 or 2, characterized in that: The hyaluronic acid-grafted glucosamine composition is prepared by the following method: 14.3 parts by weight of dicyclohexylcarbodiimide, 27.3 parts by weight of glucosamine and 200 parts by weight of water are uniformly mixed, sodium bicarbonate is added to adjust the pH to 7.5, and then 57.1-60.7 parts by weight of hyaluronic acid are added dropwise while stirring, and the addition is completed within 30 minutes. Citric acid is added to adjust the pH to 6.5, and the reaction is continued for 2 hours, followed by dehydration and drying to obtain a hyaluronic acid-grafted glucosamine composition.

5. The aqueous biomimetic lubricant with better lubricity and durability as claimed in claim 1 or 2, characterized in that: The hyaluronic acid-grafted glucosamine composition is prepared by the following method: 14.3 parts by weight of dicyclohexylcarbodiimide, 27.3 parts by weight of glucosamine and 200 parts by weight of water were uniformly mixed, sodium bicarbonate was added to adjust the pH to 7.5, and then 58.4 parts by weight of hyaluronic acid was added dropwise while stirring. The addition took 30 minutes, and citric acid was added to adjust the pH to 6.

5. The reaction was continued for 2 hours, and then dehydrated and dried to obtain a hyaluronic acid-grafted glucosamine composition.

6. A method for preparing a water-based bionic lubricant with better lubricity and durability as claimed in any one of claims 1 to 5, characterized in that The following steps are involved: (1) Prepare materials in proportion; (2) dissolving the hyaluronic acid-grafted glucosamine composition in water at room temperature to prepare an aqueous solution of the hyaluronic acid-grafted glucosamine composition, then adding hyaluronic acid and stirring evenly to obtain a solution A; (3) Under stirring conditions, dissolving β-(1,3)-glucan in water to prepare a β-(1,3)-glucan aqueous solution to obtain solution B; (4) Mixing polyethylene glycol and the remaining water uniformly to obtain a mixed solution C; (5) Under stirring conditions, solution A is slowly poured into mixed solution C. After stirring for 30 minutes, solution B is slowly poured into the mixed solution under continued stirring to obtain a transparent solution, which is the water-based bionic lubricant.

7. The method of producing a water-based biomimetic lubricant with better lubricity and durability as claimed in claim 6, characterized in that: The step (2) specifically comprises: dissolving the hyaluronic acid-grafted glucosamine composition in water at room temperature to prepare a 5 wt % aqueous solution of the hyaluronic acid-grafted glucosamine composition, then adding hyaluronic acid, and stirring evenly to obtain solution A.

8. The method of producing a water-based biomimetic lubricant with better lubricity and durability as claimed in claim 6, characterized in that: Step (3) is specifically as follows: under stirring conditions, β-(1,3)-glucan is dissolved in water to prepare a 10 wt% β-(1,3)-glucan solution to obtain solution B.

9. The method of producing a water-based biomimetic lubricant with better lubricity and durability as claimed in claim 8, characterized in that: The stirring temperature in step (3) is 55°C-65°C.

10. The method of producing a water-based biomimetic lubricant with better lubricity and durability as claimed in claim 6, characterized in that: Step (4) is specifically as follows: slowly pouring solution A into mixed solution C under stirring at 30°C-35°C, stirring for 30 minutes, and then slowly pouring solution B into the mixed solution under continued stirring to obtain a transparent solution, which is the water-based bionic lubricant.

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