Device for extracting and purifying albumin in rabbit blood

Abstract

The invention relates to the technical field of blood albumin extraction, in particular to a device for extracting and purifying albumin in rabbit blood. The centrifugal group is used for driving a test tube to rotate to centrifugally separate plasma and red blood cells from blood in the test tube, the injection group is used for independently supplying an anticoagulant, a precipitator and a purifying agent to the top end of the test tube, and the ultrafiltration group is used for filtering albumin in plasma precipitate. According to the invention, the test tubes are mounted on the centrifugal group to carry out blood and subsequent plasma centrifugal layering operation, and the injection group is mounted at the tops of the test tubes, so that different reagents are added in sequence for reaction; according to the device, the extraction and purification processes of the albumin in the blood are completed in one test tube in a centralized manner, the operation is optimized, the number of the test tubes is reduced, the operation is completed in a closed environment, and the purification degree of the albumin is improved.

Description

Technical Field

[0001] The invention relates to the technical field of blood albumin extraction, in particular to a device for extracting and purifying albumin in rabbit blood. Background Art

[0002] Application No. CN201720991929.4 discloses a centrifugal separation device for extracting fetoprotein from animal blood, comprising a shell, the inner bottom surface of the shell being mounted to a control base plate by screws, a high-speed motor being mounted above the control base plate, a centrifugal turntable being mounted on the upper end of an output shaft of the high-speed motor, the centrifugal turntable being provided with a splitting groove, the splitting groove being provided with four slideways evenly arranged from the inside to the outside, a test tube pocket being slidably mounted on the bottom of the slideway, a boss being integrally formed at the upper end of the output shaft, a weight ring being mounted on the outer end of the boss; the test tube pocket is placed through the slideway provided on the separation turntable, and then different substances are separated through different positions and rotation speeds, and at the same time, the addition of the weight ring enables the centrifuge to separate and process a single sample, thereby avoiding the problem of difficult single-tube centrifugal processing.

[0003] During the extraction and purification of blood albumin, the centrifuged and layered solutions need to be stored in different test tubes in order to react with different solutions for additives. This brings many inconveniences. For example, the complexity of the operation increases: the liquid needs to be transferred and replaced frequently, which increases the complexity and workload of the operation; each step of the operation needs to be careful to avoid contamination or loss of the sample. High resource consumption: the use of multiple test tubes also means that more experimental materials are needed. Difficulty in tracking and recording: When multiple test tubes are used, it becomes particularly important to record each step of the operation and each test tube; however, this also increases the difficulty of tracking and recording, which is prone to omissions or errors. Summary of the invention

[0004] In order to overcome the defects in the prior art, the object of the present invention is to provide a device for extracting and purifying albumin in rabbit blood to solve the problems raised in the above background technology.

[0005] To achieve the above object, the present invention provides a device for extracting and purifying albumin in rabbit blood, comprising a centrifugal group for driving a test tube to rotate and centrifuge the blood therein to separate plasma and red blood cells, an injection group for separately supplying an anticoagulant, a precipitant and a purifying agent to the top of the test tube, and an ultrafiltration group for filtering out albumin in the plasma precipitate; The centrifugal group includes a hosting seat, a claw plate sleeved on the top port of the hosting seat, and a servo motor installed inside the hosting seat for driving the claw plate to rotate; the claw plate is coaxially connected to the bottom of the test tube; The injection group comprises an injection box and a discharge sleeve which are sequentially sleeved on the outer wall of the top of the test tube from the outside to the inside, a positioning sleeve is sleeved on the central axis of the injection box and the discharge sleeve, the bottom of the positioning sleeve is snap-fitted with the top port of the test tube, the injection box is horizontally rotatably connected with the positioning sleeve, and the discharge sleeve is vertically slidably sleeved with the positioning sleeve; the interior of the injection box is divided into a plurality of spaces for storing a plurality of reagents, and the bottom inner side walls of the plurality of spaces are provided with discharge ports, a feed port is provided on the top side wall of the test tube, the feed port is arranged at the same height as the positions of the plurality of discharge ports, and a through port is provided on the bottom side wall of the discharge sleeve directly below the feed port; The top inner wall of the injection box and several spaces are provided with sliders, the top outer wall of the discharge sleeve is provided with an annular groove, and the outer arc surface of the annular groove is provided with a lifting block in the shape of a right triangle, one end of the hypotenuse of the lifting block is connected to the upper end surface of the annular groove, and the other end thereof extends to the upper part of the lower end surface of the annular groove, and the lifting block is located at the uppermost part of the half area of ​​the feed port; by grasping the positioning sleeve and rotating the injection box to make a slider slide to the lower end of the lifting block, the discharge sleeve is driven to move upward, so that the feed port is moved to overlap between the discharge port and the feed port, that is, the reagent in the discharge port is discharged into the test tube for reaction; The ultrafiltration group comprises an ultrafiltration membrane sleeved in the top port of the test tube, a pressure rod coaxially connected to the ultrafiltration membrane, and a pressure belt used for pressing the pressure rod downward to be inserted into the test tube through the central hole of the positioning sleeve.

[0006] As a further improvement of the technical solution, the outer side wall of the bottom of the test tube is provided with a plurality of grooves at equal intervals in a circular shape, and the top surface of the claw plate is provided with a plurality of claws corresponding to the plurality of grooves.

[0007] As a further improvement of the technical solution, a ring platform is provided on the top outer wall of the test tube and below the feed port, the ring platform is used to support the bottom ends of the filling box and the unloading sleeve, and a drainage hole is opened on one side wall of the ring platform that penetrates to the inner wall of the test tube.

[0008] As a further improvement of the present technical solution, the injection box has an annular box body structure and its bottom surface is inclined downward. A cap-shaped box sleeve which is sleeved with the positioning sleeve is provided on the inner port of the top surface of the injection box. The positioning sleeve is composed of a cylinder and a pressure cover at its bottom end. The pressure cover is snap-fitted with the top port of the test tube. Limiting rings are tightly sleeved on the outer wall of the cylinder of the positioning sleeve. The box sleeve overlaps the top surface of the limiting ring located above, and a nut is threadedly connected to the outer wall of the cylinder of the positioning sleeve and located above the top surface of the box sleeve.

[0009] As a further improvement of the technical solution, the inner wall of the unloading sleeve is provided with a ring in the form of a closed sleeve at the annular groove. The ring is connected to the cylindrical sleeve of the positioning sleeve and overlaps the top surface of the limiting ring located below. The distance between the two limiting rings is greater than the height dimension of the annular groove.

[0010] As a further improvement of the technical solution, the inner hole of the collar is a special-shaped hole and is matched with the lower half of the cylinder of the positioning sleeve, and a compression spring is arranged on the top surface of the collar to be sleeved with the lower half of the cylinder.

[0011] As a further improvement of the technical solution, the top surface of the injection box is provided with injection ports in several spaces, the outer sleeve of the box sleeve is provided with a sealing cover, and the bottom surface of the sealing cover is bonded with sealing gaskets corresponding to the several injection ports.

[0012] As a further improvement of the technical solution, a pressure frame which is slidably sleeved with the test tube is arranged on the top of the ultrafiltration membrane, a ring frame for supporting the ultrafiltration membrane is sleeved on the outer side of the pressure frame, a sleeve rod is vertically provided at the center of the top surface of the pressure frame, and the sleeve rod is sleeved and matched with the center hole of the positioning sleeve.

[0013] As a further improvement of the technical solution, the pressure rod and the sleeve rod have the same outer diameter and are threadedly connected at their proximal ends. Clamping blocks are symmetrically arranged at intervals at the top of the pressure rod, and the pressure belt is clamped and slidably connected to a pair of clamping blocks.

[0014] As a further improvement of the present technical solution, the bottom side walls of the hosting seat are symmetrically provided with straight plates, one end of the pressure belt is hooked and cooperated with one of the straight plates, and the other straight plate is provided with a tape reel for winding up the other end of the pressure belt, and a motor or a crank is installed at one end of the central axis of the tape reel.

[0015] Compared with the prior art, the present invention has the following beneficial effects: 1. The device for extracting and purifying albumin in rabbit blood is installed on a centrifugal group through a set test tube to perform blood and subsequent plasma centrifugal stratification operations, and an injection group is installed on the top of the test tube to add different reagents in sequence for reaction, such as an anticoagulant to prevent blood coagulation, a precipitant to precipitate albumin in plasma, and a purifying agent to purify albumin substances; and filtering and purification are performed in the test tube through an ultrafiltration group, that is, the device completes the extraction and purification processes of albumin in blood in one test tube, optimizes the operation, saves the number of test tubes, and is completed in a closed environment, thereby improving the purity of albumin.

[0016] 2. The device for extracting and purifying albumin from rabbit blood grasps the positioning sleeve and rotates the injection box to slide a slider to the lower end of the lifting block, thereby driving the unloading sleeve to move upward, causing the material passage to move to overlap between the discharge port and the feed port, that is, unloading the reagent in the discharge port into the test tube for reaction, so that the additive is reacted in a closed environment, thereby improving the quality of albumin sterilization.

[0017] 3. The device for extracting and purifying albumin in rabbit blood drives the ultrafiltration membrane to the bottom of the test tube by applying pressure to the pressure rod through the winding pressure belt. Under a certain pressure, the ultrafiltration membrane allows small molecular solutes and ions in the supernatant to pass through its pores and penetrate to the top of the membrane, while large molecules, namely albumin solutes, cannot pass through and remain under the membrane, thereby purifying the albumin macromolecules. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The accompanying drawings described herein are only for explanation purposes and are not intended to limit the scope of the present invention in any way. In addition, the shapes and proportional dimensions of the various components in the figures are only schematic, used to help understand the present invention, and are not specifically limited to the shapes and proportional dimensions of the various components of the present invention. Those skilled in the art can select various possible shapes and proportional dimensions to implement the present invention according to the teachings of the present invention.

[0019] Figure 1 It is a schematic diagram of the overall assembly structure of the present invention; Figure 2 It is a schematic diagram of the assembly structure of the test tube, the centrifugal group and the injection group of the present invention; Figure 3 This is a front view of the internal assembly of the injection group of the present invention; Figure 4 It is a schematic diagram of the internal assembly structure of the injection group of the present invention; Figure 5 It is a schematic diagram of the assembly structure of the unloading sleeve and the test tube of the present invention; Figure 6 This is a schematic diagram of the assembly structure of the positioning sleeve and the test tube of the present invention; Figure 7 It is a disassembled diagram of the test tube and centrifuge assembly of the present invention; Figure 8 It is a disassembly diagram of the positioning sleeve and the unloading sleeve of the present invention; Fig. 9 It is a schematic diagram of the full cross-section structure of the injection box of the present invention; Fig.10 This is a schematic diagram of the ultrafiltration assembly structure of the present invention; Fig.11 It is a schematic diagram of the assembly structure of the ultrafiltration membrane and the pressure rod of the present invention; The meaning of each number in the figure is: 100, test tube; 101, feed port; 102, slot; 103, ring platform; 104, drain hole; 200, centrifugal group; 210, hosting seat; 211, limit strip; 212, sleeve; 220, claw plate; 300, injection group; 310, injection box; 311, discharge port; 312, injection port; 313, box sleeve; 314, slider; 320, positioning sleeve; 321, limiting ring; 322, gland; 330, discharge sleeve; 331, material passage; 332, ring groove; 333, lifting block; 334, compression spring; 335, sleeve ring; 340, cover; 400, ultrafiltration group; 410, ultrafiltration membrane; 411, pressure frame; 420, pressure rod; 421, sleeve rod; 422, clamping block; 430, pressure belt; 431, belt reel. DETAILED DESCRIPTION

[0020] The details of the present invention can be more clearly understood by combining the accompanying drawings with the description of the specific embodiments of the present invention. However, the specific embodiments of the present invention described herein are only used for the purpose of explaining the present invention and cannot be understood as limiting the present invention in any way. Under the guidance of the present invention, technicians can conceive of any possible variations based on the present invention, which should be regarded as falling within the scope of the present invention. The terms "installation" and "connection" should be understood in a broad sense, which can be directly connected or indirectly connected through an intermediate medium.

[0021] The terms "central axis", "vertical", "horizontal", "front", "back", "up", "down", "left", "right", "top", "bottom", "inside", "outside" and the like used herein to indicate positions or positional relationships are based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the equipment or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, in the description of the present invention, the meaning of "several" is two or more, unless otherwise clearly and specifically defined.

[0022] See also Figure 1-Figure 11As shown, the present invention provides a device for extracting and purifying albumin in rabbit blood, comprising a centrifugal group 200 for driving a test tube 100 to rotate and centrifuge the blood therein to separate plasma and red blood cells, an injection group 300 for separately supplying an anticoagulant, a precipitant and a purifying agent to the top of the test tube 100, and an ultrafiltration group 400 for filtering out albumin in the plasma precipitate; firstly, an anticoagulant is added to the test tube 100 to prevent blood coagulation, then the centrifugal group 200 is started to drive the test tube 100 to rotate, and the blood is centrifuged to separate plasma and red blood cells; then the red blood cell layer is discharged and the plasma layer is left, and then the test tube 100 is charged with an ultrafiltration group 400. 0 to precipitate albumin in the plasma; then the centrifugal group 200 is started to centrifugally separate the albumin in the precipitate and the supernatant; then a purifying agent, i.e., ion exchange resin particles, is added to the test tube to separate the ions and other impurities therein; then the ultrafiltration group 400 is used to filter out the supernatant, while the albumin is left at the bottom of the test tube 100, and then the supernatant is discharged to obtain purified albumin; that is, this device completes the extraction and purification process of albumin in the blood in one test tube 100, optimizes the operation, saves the number of test tubes, and completes it in a closed environment, thereby improving the purity of albumin.

[0023] Specifically, the centrifugal group 200 includes a hosting seat 210, a claw plate 220 sleeved on the top port of the hosting seat 210, and a servo motor installed inside the hosting seat 210 for driving the claw plate 220 to rotate; a plurality of openings are opened on the bottom outer wall of the hosting seat 210 for heat dissipation and exhaust; the claw plate 220 is coaxially connected to the bottom of the test tube 100; The outer wall of the bottom of the test tube 100 is provided with a plurality of slots 102 at equal intervals in an annular shape, and the top surface of the claw plate 220 is provided with a plurality of claws corresponding to the plurality of slots 102, so that the coaxial connection between the test tube 100 and the claw plate 220 is stable, which is conducive to the centrifugal movement of the liquid in the test tube 100 and the stratification; A ring platform 103 is provided on the top outer wall of the test tube 100 and below the feed port 101. The test tube 100 is made of transparent plastic as an integrally formed structure so that the stratification of the liquid therein can be observed; the ring platform 103 is used to support the bottom ends of the filling box 310 and the unloading sleeve 330, and plays a role of limiting support; a drainage hole 104 is provided on one side wall of the ring platform 103 and penetrates through the inner wall of the test tube 100. By inserting a plastic tube and equipping it with a syringe, the stratified liquid in the test tube 100 can be correspondingly pumped out.

[0024] Specifically, the injection group 300 includes an injection box 310 and a discharge sleeve 330 which are sequentially sleeved on the outer wall of the top of the test tube 100 from the outside to the inside. A positioning sleeve 320 is sleeved on the central axis of the injection box 310 and the discharge sleeve 330. The structure of the injection group 300 is made of plastic and is made into an integral structure. The bottom of the positioning sleeve 320 is snap-fitted with the top port of the test tube 100. By pressing the positioning sleeve 320 to block the top port of the test tube 100, liquid leakage during centrifugal movement is prevented. The inside of the injection box 310 is divided into a plurality of spaces for storing a plurality of reagents, and the bottom inner side walls of the plurality of spaces are provided with discharge ports 311, and the top side wall of the test tube 100 is provided with a feed port 101, and the position of the feed port 101 is arranged at the same height as the positions of the plurality of discharge ports 311; The filling box 310 has an annular box structure and its bottom surface is tilted downward, so that the reagents gather on its inner wall; since the bottom of the filling box 310 is an inclined structure, the reagents in each space can automatically slide out and fall into the test tube 100 when the discharge port 311 is connected to the feed port 101.

[0025] Furthermore, a cap-shaped box sleeve 313 which is sleeved with the positioning sleeve 320 is provided on the inner port on the top surface of the injection box 310. The positioning sleeve 320 is composed of a cylinder and a pressure cover 322 at its bottom end. The pressure cover 322 is snap-fitted with the top port of the test tube 100. The cylindrical outer wall of the positioning sleeve 320 is tightly sleeved with limiting rings 321. The box sleeve 313 overlaps the top surface of the limiting ring 321 located above, and the cylindrical outer wall of the positioning sleeve 320 is threadedly connected with a nut located above the top surface of the box sleeve 313, so that the injection box 310 is horizontally rotatably connected with the positioning sleeve 320.

[0026] Furthermore, the bottom side wall of the discharge sleeve 330 is provided with a material through hole 331 located directly below the feed port 101; that is, in a normal state, the discharge sleeve 330 is located between the test tube 100 and the injection box 310 to block the transport of reagents; when the discharge sleeve 330 is moved upward, the material through hole 331, the discharge port 311 and the feed port 101 coincide with each other and the discharge can be carried out; The top inner wall of the injection box 310 and several spaces are provided with sliders 314, the top outer wall of the discharge sleeve 330 is provided with an annular groove 332, and the inner wall of the discharge sleeve 330 and the annular groove 332 are provided with a ring 335 in a closed sleeve, the ring 335 is connected with the cylindrical sleeve of the positioning sleeve 320 and overlaps the top surface of the limiting ring 321 located below, and the distance between the two limiting rings 321 is greater than the height dimension of the annular groove 332, so that the ring 335 has a rising space so that the material through port 331 and the discharge port 311 and the feed port 101 coincide with each other.

[0027] Furthermore, the inner hole of the collar 335 is a special-shaped hole and is matched with the lower half of the cylinder of the positioning sleeve 320, so that the unloading sleeve 330 and the positioning sleeve 320 are vertically slidably sleeved; a compression spring 334 sleeved with the lower half of the cylinder is arranged on the top surface of the collar 335, and the unloading sleeve 330 moves downward and resets by the rebound of the compression spring 334 to block the unloading of the reagent; The outer arc surface of the annular groove 332 is provided with a lifting block 333 in the shape of a right triangle, one end of the hypotenuse of the lifting block 333 is connected to the upper end surface of the annular groove 332, and the other end thereof extends to above the lower end surface of the annular groove 332, and the vertical height of the lifting block 333 is the travel distance of the material passage 331, so that it coincides with the discharge port 311 and the feed port 101; the lifting block 333 is located at the uppermost part of the half area of ​​the material passage 331; when the material injection box 310 is rotated to make the slider 314 slide away from the lower end of the lifting block 333, the unloading sleeve 330 can instantly drop under the action of the rebound force of the compression spring 334, and separate the discharge port 311 and the feed port 101, until the next slider 314 rotates to the lower end of the lifting block 333, opening up another discharge port 311 and the feed port 101 to complete the unloading of another reagent; By grasping the positioning sleeve 320 and rotating the injection box 310, a slider 314 slides to the lower end of the lifting block 333, thereby driving the unloading sleeve 330 to move upward, causing the material passage 331 to move to overlap between the discharge port 311 and the feed port 101, that is, the reagent in the discharge port 311 is unloaded into the test tube 100 for reaction.

[0028] Furthermore, injection ports 312 are provided on the top surface of the injection box 310 and in several spaces for injecting different reagents into the several spaces of the injection box 310; a sealing cover 340 is provided on the outside of the box sleeve 313, and sealing gaskets corresponding to the several injection ports 312 are bonded to the bottom surface of the sealing cover 340, and the sealing gaskets are made of silicone so as to block the injection ports 312 to form a sealed state.

[0029] Specifically, the ultrafiltration group 400 includes an ultrafiltration membrane 410 sleeved in the top port of the test tube 100, a pressure rod 420 coaxially connected to the ultrafiltration membrane 410, and a pressure belt 430 for pressing the pressure rod 420 downward and inserting it from the center hole of the positioning sleeve 320 into the test tube 100; under a certain pressure, the ultrafiltration membrane 410 allows small molecular solutes and ions to pass through its pores and penetrate to the top of the membrane, while preventing large molecules, namely albumin solutes, from passing through and remaining below the membrane, thereby purifying the albumin macromolecules.

[0030] Furthermore, a pressing frame 411 is provided on the upper side of the ultrafiltration membrane 410 and is slidably sleeved with the test tube 100. A ring frame for supporting the ultrafiltration membrane 410 is sleeved on the outer side of the pressing frame 411. The ultrafiltration membrane 410 is clamped and flattened by the pressing frame 411 and the ring frame to filter the solution in the test tube 100 under pressure. A sleeve rod 421 is vertically provided at the center of the top surface of the pressure frame 411, and the sleeve rod 421 is sleeved and matched with the center hole of the positioning sleeve 320; the pressure rod 420 and the sleeve rod 421 have the same outer diameter and are threadedly connected at the near end of the two, so that the two are easy to disassemble and assemble; the top of the pressure rod 420 is symmetrically provided with clamping blocks 422 at intervals, and the pressure belt 430 is clamped and slid with a pair of clamping blocks 422 to prevent the pressure belt 430 from sliding sideways; the outer wall of the hosting seat 210 is vertically integrated and provided with a sleeve 212, and the pressure rod 420 is removed and inserted into the sleeve 212 for storage, so that the overall space occupied by the device is reduced.

[0031] Furthermore, the bottom side wall of the hosting seat 210 is symmetrically provided with straight plates, so that the hosting seat 210 is placed stably; one end of the pressing belt 430 is hooked and matched with one of the straight plates, that is, a circular ring is fastened to one end of the pressing belt 430, and a vertical ring is symmetrically provided on the top surface of the straight plate, and the circular ring is fixedly connected to a pair of vertical rings by pins; A tape reel 431 for winding up the other end of the pressing tape 430 is provided on the other straight plate, and a motor or a crank is installed at one end of the central axis of the tape reel 431, thereby driving the tape reel 431 to wind up the pressing tape 430 and the pressure rod 420 presses the ultrafiltration membrane 410 down to the bottom of the test tube 100 to complete the osmotic filtration of small molecules in the solution in the test tube; an inverted L-shaped limiting strip 211 is symmetrically arranged on the other straight plate, and support frames plugged into a pair of limiting strips 211 are sleeved at both ends of the central axis of the tape reel 431, so that the tape reel 431 can rotate stably to rewind the tape.

[0032] When the device for extracting and purifying albumin from rabbit blood of the present invention is used, the extracted rabbit blood is poured into the test tube 100, and then the test tube 100 is inserted into the hosting seat 210, and the injection group 300 filled with different reagents is inserted into the top of the test tube 100 as a whole, and the positions of the anticoagulant, the precipitant and the purifying agent are successively close to the feed port 101; Then, by grasping the positioning sleeve 320 and rotating the injection box 310, a slider 314 slides to the lower end of the lifting block 333, and the discharge sleeve 330 is driven to move upward, so that the material passage 331 moves to overlap between the discharge port 311 and the feed port 101, that is, the anticoagulant in the discharge port 311 is discharged into the test tube 100 to prevent blood coagulation. This process is the discharge process, and this section of the working process is replaced by this in the following text; Then, the centrifugal group 200 is started to drive the test tube 100 to rotate, and the blood is centrifuged to separate the plasma and red blood cells; then, the red blood cell layer is discharged from the drainage hole 104 through the combination of a plastic tube and a syringe, and the plasma layer is left; and the unloading process is performed again to add a precipitant into the test tube 100 to precipitate the albumin in the plasma; Then, the centrifugal group 200 is started to centrifuge and separate the albumin and supernatant in the precipitate; the unloading process is performed again to add the purifying agent, i.e., ion exchange resin particles, into the test tube to separate the ions and other impurities therein; and the ultrafiltration membrane 410 is pressed to the bottom of the test tube 100 by the pressure rod 420 applied by the winding pressure belt 430. Under a certain pressure, the ultrafiltration membrane 410 allows the small molecular solutes and ions in the supernatant to pass through its pores and penetrate to the top of the membrane, while the macromolecule, i.e., the albumin solute, cannot pass through and remains below the membrane, thereby purifying the albumin macromolecule substance; Then, the supernatant is discharged from the drainage hole 104 to obtain the purified albumin; and the purified albumin is taken out and collected; that is, the device completes the extraction and purification process of albumin in the blood in one test tube 100, optimizes the operation, saves the number of test tubes, and completes it in a closed environment, thereby improving the purity of the albumin.

[0033] It should be noted that the above embodiments are only for illustrating the technical concept and features of the present invention, and their purpose is to enable people familiar with the technology to understand the content of the present invention and implement it accordingly, and they cannot be used to limit the protection scope of the present invention. Any equivalent changes or modifications made according to the spirit of the present invention should be included in the protection scope of the present invention.

Claims

1. A device for extracting and purifying albumin from rabbit blood, characterized in that: It comprises a centrifugation group (200) for driving the test tube (100) to rotate and centrifuge the blood therein to separate plasma and red blood cells, an injection group (300) for separately supplying an anticoagulant, a precipitant and a purifying agent to the top of the test tube (100), and an ultrafiltration group (400) for filtering out albumin in the plasma precipitate; The centrifugal group (200) comprises a hosting seat (210), a claw plate (220) sleeved on a top port of the hosting seat (210), and a servo motor installed inside the hosting seat (210) for driving the claw plate (220) to rotate; the claw plate (220) is coaxially connected to the bottom of the test tube (100); The injection group (300) comprises an injection box (310) and a discharge sleeve (330) which are sequentially sleeved on the outer wall of the top of the test tube (100) from the outside to the inside. A positioning sleeve (320) is sleeved on the central axis of the injection box (310) and the discharge sleeve (330). The bottom of the positioning sleeve (320) is snap-fitted with the top end of the test tube (100). The injection box (310) is horizontally rotatably connected to the positioning sleeve (320). The discharge sleeve (330) is rotatably connected to the positioning sleeve (320). ) vertically sliding sleeve connection; the inside of the injection box (310) is divided into a plurality of spaces for storing a plurality of reagents, and the bottom inner side walls of the plurality of spaces are each provided with a discharge port (311); the top side wall of the test tube (100) is provided with a feed port (101), the position of the feed port (101) is arranged at the same height as the positions of the plurality of discharge ports (311), and the bottom side wall of the discharge sleeve (330) is provided with a feed port (331) located directly below the feed port (101); The top inner wall of the injection box (310) and the plurality of spaces are provided with sliders (314), the top outer wall of the discharge sleeve (330) is provided with an annular groove (332), and the outer arc surface of the annular groove (332) is provided with a lifting block (333) in the form of a right triangle, one end of the hypotenuse of the lifting block (333) is connected to the upper end surface of the annular groove (332), and the other end thereof extends to above the lower end surface of the annular groove (332), and the lifting block (333) is provided with a plurality of sliders (314) on the top inner wall of the injection box (310), the top outer wall of the discharge sleeve (330) is provided with an annular groove (332), and the outer arc surface of the annular groove (332) is provided with a lifting block (333) in the form of a right triangle. 33) is located at the top of the half area of ​​the material passage opening (331); by grasping the positioning sleeve (320) and rotating the injection box (310), a slider (314) slides to the lower end of the lifting block (333), thereby driving the unloading sleeve (330) to move upward, so that the material passage opening (331) moves to overlap between the discharge opening (311) and the feed opening (101), that is, the reagent in the discharge opening (311) is discharged into the test tube (100) for reaction; The ultrafiltration group (400) comprises an ultrafiltration membrane (410) sleeved in the top port of the test tube (100), a pressure rod (420) coaxially connected to the ultrafiltration membrane (410), and a pressure belt (430) used to press the pressure rod (420) downward and insert it from the central hole of the positioning sleeve (320) into the test tube (100).

2. The device for extracting and purifying albumin from rabbit blood according to claim 1, characterized in that: The outer wall of the bottom of the test tube (100) is provided with a plurality of slots (102) at equal intervals in a circular shape, and the top surface of the claw plate (220) is provided with a plurality of claws corresponding to and plugged into the plurality of slots (102).

3. The device for extracting and purifying albumin from rabbit blood according to claim 2, characterized in that: A ring platform (103) is provided on the top outer wall of the test tube (100) and below the feed port (101); the ring platform (103) is used to support the bottom ends of the injection box (310) and the discharge sleeve (330); and a drainage hole (104) is provided on one side wall of the ring platform (103) that penetrates to the inner wall of the test tube (100).

4. The device for extracting and purifying albumin from rabbit blood according to claim 3, characterized in that: The injection box (310) is an annular box body structure and its bottom surface is inclined downward. A cap-shaped box sleeve (313) is provided on the inner port of the top surface of the injection box (310) and is sleeved with the positioning sleeve (320). The positioning sleeve (320) is composed of a cylinder and a pressure cover (322) at its bottom end. The pressure cover (322) is snap-fitted with the top port of the test tube (100). The outer wall of the cylinder of the positioning sleeve (320) is tightly sleeved with a limiting ring (321). The box sleeve (313) is overlapped with the top surface of the limiting ring (321) located above, and the outer wall of the cylinder of the positioning sleeve (320) and the top surface of the box sleeve (313) are threadedly connected to a nut.

5. The device for extracting and purifying albumin from rabbit blood according to claim 4, characterized in that: A collar (335) is provided on the inner wall of the discharge sleeve (330) at the annular groove (332) in a closed sleeve manner. The collar (335) is sleeved with the cylinder of the positioning sleeve (320) and overlaps the top surface of the limiting ring (321) located below. The distance between the two limiting rings (321) is greater than the height dimension of the annular groove (332).

6. The device for extracting and purifying albumin from rabbit blood according to claim 5, characterized in that: The inner hole of the collar (335) is a special-shaped hole and is adapted to fit the lower half of the cylinder of the positioning sleeve (320). A compression spring (334) sleeved with the lower half of the cylinder is provided on the top surface of the collar (335).

7. The device for extracting and purifying albumin from rabbit blood according to claim 6, characterized in that: The top surface of the injection box (310) and several spaces are provided with injection ports (312), the outer sleeve of the box sleeve (313) is provided with a sealing cover (340), and the bottom surface of the sealing cover (340) is bonded with sealing pads corresponding to the several injection ports (312).

8. The device for extracting and purifying albumin from rabbit blood according to claim 7, characterized in that: A pressing frame (411) is provided on the upper side of the ultrafiltration membrane (410) and is slidably sleeved with the test tube (100). A ring frame for supporting the ultrafiltration membrane (410) is sleeved on the outer side of the pressing frame (411). A sleeve rod (421) is vertically provided at the center of the top surface of the pressing frame (411). The sleeve rod (421) is sleeved and matched with the center hole of the positioning sleeve (320).

9. The device for extracting and purifying albumin from rabbit blood according to claim 8, characterized in that: The pressure rod (420) and the sleeve rod (421) have the same outer diameter and are threadedly connected at their proximal ends. Clamping blocks (422) are symmetrically arranged at intervals at the top end of the pressure rod (420). The pressure belt (430) is clamped and slidably connected to a pair of clamping blocks (422).

10. The device for extracting and purifying albumin from rabbit blood according to claim 9, characterized in that: The bottom side walls of the hosting seat (210) are symmetrically provided with straight plates, one end of the pressing belt (430) is hooked and matched with one of the straight plates, and the other straight plate is provided with a belt reel (431) for winding up the other end of the pressing belt (430), and a motor or a crank is installed at one end of the central axis of the belt reel (431).

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