Green and environment-friendly soil conditioner

Abstract

The invention belongs to the technical field of soil conditioners, and particularly relates to a green and environment-friendly soil conditioner. The green and environment-friendly soil conditioner comprises a biopesticide component, the biopesticide component is formed by compounding paecilomyces lilacinus and pydiflumetofen or oxymatrine, and the spore content of the paecilomyces lilacinus is 20 billion live spores / g. The green and environment-friendly soil conditioner disclosed by the invention comprises the biopesticide component and the potassium humate, so that the green and environment-friendly soil conditioner not only can prevent and treat crop diseases, but also can condition soil and enhance the stress resistance of crops, and is beneficial to the growth of the crops and increase the yield of the crops.

Description

Technical Field

[0001] The invention belongs to the technical field of soil conditioners, and in particular relates to a green and environment-friendly soil conditioner. Background Art

[0002] Potassium humate is a highly efficient organic potassium fertilizer with the following functions and effects: improving soil physical properties, improving soil aggregate structure, reducing soil compaction; improving fertilizer slow-release properties, increasing soil fertilizer and water retention capacity; promoting the decomposition of artificial or natural toxic substances; affecting cell metabolism, improving crop respiration and photosynthesis, and enhancing crop resistance to drought, drought and disease; strengthening roots and increasing yields, improving crop quality, and increasing the sweetness of fruits and vegetables. In addition, potassium humate can be mixed with pesticides to improve drug efficacy and inhibit residues.

[0003] Paecilomyces lilacinus is a biocontrol agent that can parasitize the eggs, larvae and adults of plant pathogenic nematodes, such as root-knot nematodes and cyst nematodes, when conditions are suitable. Immature eggs, in particular, are more susceptible to parasitism by Paecilomyces lilacinus. Paecilomyces lilacinus usually parasitizes nematodes by directly piercing and invading with hyphae or conidia, or by producing special structures on the surface of the nematodes for adsorption and infection. In this process, the chitinase and protease secreted by the fungus outside the cell play an extremely important role, which can digest the chitin layer of the eggshell, degrade proteins, and then infect, and completely destroy and utilize the substances in the nematode's body. In addition, Paecilomyces lilacinus can also prevent and control plant fungal diseases. At present, the registered pesticide categories of Paecilomyces lilacinus are insecticides, nematicides and fungicides.

[0004] Fluoxathiapiprolin is a pyridine amide fungicide that acts on succinate dehydrogenase. It acts on succinate dehydrogenase, one of the hubs connecting oxidative phosphorylation and electron transfer in bacteria, leading to a disorder of the tricarboxylic acid cycle, hindering its energy metabolism, and then inhibiting the growth of pathogens, leading to their death, thereby achieving the purpose of preventing and controlling diseases. Fluoxathiapiprolin has a high preventive effect on diseases caused by pathogens such as Botrytis cinerea, Sclerotinia sclerotiorum, Fusarium, powdery mildew and Coryneformis. In addition, Fluoxathiapiprolin also has high nematicidal activity.

[0005] In the process of preventing and controlling plant diseases and insect pests, using a single active ingredient alone can easily lead to the generation and development of pest resistance, resulting in reduced or even ineffective pest control effects. Compounding different active ingredients can expand the control spectrum, improve control effects, and alleviate the problem of resistance. However, not all ingredients can achieve the above effects, and screening is required. Summary of the invention

[0006] The purpose of the present invention is to provide a green and environmentally friendly soil conditioner, which can effectively prevent and control nematodes in the soil and condition the soil.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] A green and environmentally friendly soil conditioner, comprising a biological pesticide component, wherein the biological pesticide component is compounded by Paecilomyces lilacinus and fluopicolide or oxymatrine, and the spore content of Paecilomyces lilacinus is 20 billion viable spores / g.

[0009] Preferably, the mass ratio of the Paecilomyces lilacinus to fluopicolide is 1-80:6-1.

[0010] Preferably, the mass ratio of the Paecilomyces lilacinus to oxymatrine is 1:15-1.

[0011] Preferably, in terms of mass percentage, the biopesticide component accounts for 2-8% of the total mass of the green and environmentally friendly soil conditioner.

[0012] Preferably, the green and environmentally friendly soil conditioner comprises, by mass percentage, 2-8% of biological pesticide components, 30-55% of potassium humate, 3-6% of sodium lignin sulfonate, 2-5% of calcium dodecylbenzene sulfonate and the remainder of inert filler.

[0013] Preferably, the inert filler is selected from one or more of attapulgite, bentonite, white carbon black, light calcium carbonate, kaolin and talc.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] (1) The single-dose indoor toxicity test found that Paecilomyces lilacinus, fluopicolide and oxymatrine were effective against the southern root-knot nematode LC 50 They were 163.5007mg / L, 10.3920mg / L and 14.1685mg / L respectively. After the indoor compound test, it was found that within the range of 1-80:6-1, the co-toxicity coefficient of the compound combination of Paecilomyces lilacinus and fluopicolide hydroxylamide against southern root-knot nematodes was greater than 120, showing a synergistic effect; within the range of 1:15-1, the co-toxicity coefficient of the compound combination of Paecilomyces lilacinus and oxymatrine against southern root-knot nematodes was greater than 120, showing a synergistic effect. Based on this, compounding Paecilomyces lilacinus with fluopicolide hydroxylamide or oxymatrine can improve the control effect of crop pests, help alleviate the generation and development of pest resistance, and reduce the risk of resistance.

[0016] (2) The green and environmentally friendly soil conditioner of the present invention comprises biological pesticide components and potassium humate, which can not only prevent and treat crop diseases, but also condition the soil, enhance the stress resistance of crops, help the growth of crops and increase crop yields. DETAILED DESCRIPTION

[0017] The technical solution of the patent of this invention is clearly and completely described below.

[0018] 1. Single-dose indoor toxicity test

[0019] 1.1 Test Agents

[0020] 20 billion live spores / g of Paecilomyces lilacinus (Fujian Kaili Biological Products Co., Ltd.), 98% fluopicolide technical (Syngenta Nantong Crop Protection Co., Ltd.), 98% oxymatrine technical (Shanghai Yuanye Biotechnology Co., Ltd.)

[0021] 1.2 Test Target

[0022] The southern root-knot nematode (Meloidogyne incognita) was collected from the cucumber rhizosphere in the vegetable planting area of ​​Jiangkou Town, Guigang City, Guangxi Zhuang Autonomous Region, and was identified and preserved by the Plant Nematode Research Laboratory of the College of Agriculture of Guangxi University. It was cultured in the laboratory to obtain the second-instar larvae of the same age.

[0023] 1.3 Preparation of medicine

[0024] 20 billion live spores / g of Paecilomyces lilacinus (100% by mass) were directly prepared into a 5000 mg / L spore suspension with a 0.05% Tween-80 aqueous solution as the mother solution; the remaining agents were dissolved in dimethyl sulfoxide after 100% reduction, and then diluted with a 0.05% Tween-80 aqueous solution to a 5000 mg / L mother solution. Each single dose of the mother solution was further diluted with a 0.05% Tween-80 aqueous solution to a series of concentrations shown in Table 1 below.

[0025] Table 1 Single dose series concentration

[0026]

[0027] 1.4 Chemical treatment

[0028] Resuspend the cultured second-instar larvae to 200 heads / mL with water, and use a pipette to draw 3mL of the drug solution and mix it evenly with an equal amount of nematode suspension. Pipette a certain volume of the mixed solution into the small wells of a 24-well biochemical test plate, cover it, and culture it at a constant temperature of 25°C for 36 hours. Repeat the treatment with each mass concentration of drug solution 4 times, and set the treatment with 0.05% Tween-80 aqueous solution as a control.

[0029] 1.5 Investigation and Analysis

[0030] Take 1 mL of the mixed solution from each treatment and observe the death of nematodes under a dissecting microscope. The number of nematodes observed in each repetition is no less than 100. Record the total number of nematodes investigated and the number of dead nematodes. Calculate the mortality rate and corrected mortality rate of each treatment based on the survey data. The criterion for determining the death of nematodes is: the nematodes are rigid and cannot bend or move when touched with a hairpin.

[0031]

[0032] 1.6 Test results

[0033] The LC of a single agent was calculated by regression analysis based on the logarithmic value of the agent concentration and the corresponding corrected mortality probability of pathogenic nematodes. 50 , the results are shown in Table 2.

[0034] Table 2 Results of indoor toxicity test of each single agent against southern root-knot nematode

[0035]

[0036] As shown in Table 2, the LC of P. lilacinus, fluopicolide and oxymatrine against the southern root-knot nematode 50 They are 163.5007 mg / L, 10.3920 mg / L and 14.1685 mg / L respectively.

[0037] 2. Compatibility test of chemical agents with Paecilomyces lilacinus

[0038] 2.1 Test Agents

[0039] 98% fluopicolide technical (Syngenta Nantong Crop Protection Co., Ltd.), 96% oxymatrine technical (Bayer AG)

[0040] 2.2 Preparation of medicine

[0041] After the agent is completely dissolved in dimethyl sulfoxide, it is diluted with 0.05% Tween-80 aqueous solution to a 10000 mg / L stock solution. Each single-dose stock solution is further diluted with 0.05% Tween-80 aqueous solution to a series of concentrations as shown in Table 1.

[0042] 2.3 Test methods

[0043] Take 9 mL of PDA culture cooled to 55°C and 1 mL of drug solution, mix them evenly, pour them into a culture dish with a diameter of 9 cm, and form a drug-containing plate after cooling. Each mass concentration of drug solution treatment is repeated 5 times, and the treatment with an equal amount of 0.05% Tween-80 aqueous solution is set as the control.

[0044] 20 billion live spores / g of Paecilomyces lilacinus (100% by mass) were directly prepared into a 5000 mg / L spore suspension with a 0.05% Tween-80 aqueous solution. 100 μL of the spore suspension was taken with a pipette and evenly spread on the surface of the drug-containing plate and the control plate, and placed in a constant temperature incubator at 37°C for cultivation. After 4 days, the growth of Paecilomyces lilacinus on the plate was observed, and the number of colonies was counted and recorded.

[0045] Results: At low concentrations, fluopicolide and oxymatrine had good compatibility with Paecilomyces lilacinus, and Paecilomyces lilacinus could grow normally. With the increase of fluopicolide and oxymatrine concentrations, the growth of Paecilomyces lilacinus began to be gradually inhibited; when the concentration reached 40 mg / L, the growth of Paecilomyces lilacinus was significantly inhibited, and the number of colonies decreased significantly; when the concentration reached 80 mg / L, Paecilomyces lilacinus could hardly grow. Comprehensively considering the LC of fluopicolide and oxymatrine against southern root-knot nematodes 50 The value of fluopicolide and oxymatrine can be used as chemical factors in the compound agent of Pseudomonas lilacinus.

[0046] 3. Indoor toxicity test of compound pesticides against southern root-knot nematodes

[0047] 3.1 Test Agents

[0048] 20 billion live spores / g of Paecilomyces lilacinus (Fujian Kaili Biological Products Co., Ltd.), 98% fluopicolide technical (Syngenta Nantong Crop Protection Co., Ltd.), 96% oxymatrine technical (Bayer AG)

[0049] 3.2 Test Target

[0050] The southern root-knot nematode (Meloidogyne incognita) was collected from the cucumber rhizosphere in the vegetable planting area of ​​Jiangkou Town, Guigang City, Guangxi Zhuang Autonomous Region, and was identified and preserved by the Plant Nematode Research Laboratory of the College of Agriculture of Guangxi University. It was cultured in the laboratory to obtain the second-instar larvae of the same age.

[0051] 3.3 Preparation of medicines

[0052] 20 billion live spores / g of Paecilomyces lilacinus (100% by mass) are directly prepared into a 5000 mg / L spore suspension with a 0.05% Tween-80 aqueous solution as the mother solution; the remaining agents are completely dissolved in dimethyl sulfoxide and then diluted with a 0.05% Tween-80 aqueous solution to a 5000 mg / L mother solution. Multiple groups of ratios are set, and each ratio mixture is diluted with a 0.05% Tween-80 aqueous solution to a series of concentrations shown in Table 3 below.

[0053] Table 3 Proportion of mixed agent series concentration

[0054]

[0055] 3.4 Chemical treatment

[0056] Resuspend the cultured second-instar larvae to 200 heads / mL with water, and use a pipette to draw 3mL of the drug solution and mix it evenly with an equal amount of nematode suspension. Pipette a certain volume of the mixed solution into the small wells of a 24-well biochemical test plate, cover it, and culture it at a constant temperature of 25°C for 36 hours. Repeat the treatment with each mass concentration of drug solution 4 times, and set the treatment with 0.05% Tween-80 aqueous solution as a control.

[0057] 3.5 Investigation and Analysis

[0058] Take 1 mL of the mixed solution from each treatment and observe the death of nematodes under a dissecting microscope. The number of nematodes observed in each repetition is no less than 100. Record the total number of nematodes investigated and the number of dead nematodes. Calculate the mortality rate and corrected mortality rate of each treatment based on the survey data. The criterion for determining the death of nematodes is: the nematodes are rigid and cannot bend or move when touched with a hairpin.

[0059]

[0060] 3.6 Test results

[0061] The LC of each mixture was calculated by regression analysis based on the logarithmic value of the concentration of the agent and the corresponding corrected mortality probability of the pathogenic nematodes. 50 The co-toxicity coefficient (CTC value) of the mixture was calculated using Paecilomyces lilacinus as the standard agent according to Sun Yunpei's method, and the synergistic effect of the agents was evaluated based on the calculated co-toxicity coefficient (CTC value). The results are shown in Table 4-5.

[0062] Table 4 Results of indoor toxicity test of the complex of Paecilomyces lilacinus and fluopicolide against incognita root-knot nematodes

[0063]

[0064] Table 5 Results of indoor toxicity test of the complex of Paecilomyces lilacinus and oxymatrine against incognita root-knot nematodes

[0065]

[0066] It can be seen from Tables 4 and 5 that in the range of 1-80:6-1, the co-toxicity coefficient of the combination of Paecilomyces lilacinus and fluopicolide to southern root-knot nematodes was greater than 120, showing a synergistic effect; in the range of 1:15-1, the co-toxicity coefficient of the combination of Paecilomyces lilacinus and oxymatrine to southern root-knot nematodes was greater than 120, showing a synergistic effect.

[0067] In summary, the present invention compounds Paecilomyces lilacinus with fluopicolide or oxymatrine, which can improve the control effect on crop pests, help alleviate the generation and development of pest resistance, and reduce the risk of resistance.

[0068] 4. Green and environmentally friendly soil conditioner formula 1

[0069] A green and environment-friendly soil conditioner is prepared from the following ingredients, measured by mass percentage: 3% of a biological pesticide component, 42% of potassium humate, 3.75% of sodium lignin sulfonate, 4% of calcium dodecylbenzene sulfonate, and the remainder of white carbon black; wherein the biological pesticide component is compounded from 20 billion live spores / g of Paecilomyces lilacinus and fluopicolide in a mass ratio of 20:1.

[0070] 5. Green and environmentally friendly soil conditioner formula 2

[0071] A green and environment-friendly soil conditioner is prepared from the following ingredients, measured by mass percentage: 3% of a biological pesticide component, 48% of potassium humate, 5% of sodium lignin sulfonate, 3% of calcium dodecylbenzene sulfonate, and the remainder of attapulgite; wherein the biological pesticide component is compounded from 20 billion live spores / g of Paecilomyces lilacinus and oxymatrine in a mass ratio of 1:15.

Claims

1. A green and environmentally friendly soil conditioner, characterized in that: The green and environmentally friendly soil conditioner comprises a biological pesticide component, wherein the biological pesticide component is compounded by Paecilomyces lilacinus and fluopicolide or oxymatrine, and the spore content of Paecilomyces lilacinus is 20 billion live spores / g.

2. The biopesticide according to claim 1, characterized in that: The mass ratio of Paecilomyces lilacinus to fluopicolide is 1-80:6-1.

3. The biopesticide according to claim 1, characterized in that: The mass ratio of the Paecilomyces lilacinus to oxymatrine is 1:15-1.

4. The green and environmentally friendly soil conditioner according to claim 1, characterized in that: In terms of mass percentage, the biological pesticide component accounts for 2-8% of the total mass of the green and environmentally friendly soil conditioner.

5. The green and environmentally friendly soil conditioner according to claim 1, characterized in that: Measured by mass percentage, the green and environmentally friendly soil conditioner comprises 2-8% of biological pesticide components, 30-55% of potassium humate, 3-6% of sodium lignin sulfonate, 2-5% of calcium dodecylbenzene sulfonate and the remainder of inert filler.

6. The green and environmentally friendly soil conditioner according to claim 1, characterized in that: The inert filler is selected from one or more of attapulgite, bentonite, white carbon black, light calcium carbonate, kaolin and talc.

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