Drug inhibitory concentration detection device and detection method

Abstract

The invention provides a drug inhibitory concentration detection device and method. The drug inhibitory concentration detection device comprises a machine shell, a supporting frame and a rotating mechanism. The rotating mechanism comprises a containing rotating disc, a plurality of culture bottles placed on the containing rotating disc and a driving motor, a plurality of containing grooves used for containing the culture bottles are evenly distributed in the top face of the containing rotating disc along the circumferential side wall of the containing rotating disc, and a heating assembly is arranged on the containing rotating disc; an avoiding hole for communicating the inner cavity with the outside is formed in the top of the machine shell, a culture bottle is placed in or moved out of the inner cavity, and a sealing cover plate covers the position, located on the avoiding hole, of the machine shell; and a detection assembly for detecting the concentration of bacteria in the culture bottle is arranged on the support frame. The drug inhibitory concentration detection device and the detection method provided by the invention realize real-time automatic monitoring of inhibition conditions of a plurality of drugs to a certain bacterium or sensitivity of a plurality of bacteria to the same drug, effectively improve drug inhibitory concentration detection efficiency and accuracy, and facilitate acceleration of a new antibacterial drug research process.

Description

Technical Field

[0001] The present invention belongs to the technical field of medical devices, and more specifically, relates to a drug antibacterial concentration detection device and a detection method. Background Art

[0002] Infectious diseases are one of the major diseases facing human beings today. Antibiotics can control many bacterial infections and effectively reduce the mortality rate of various bacterial infections. However, factors such as the global overuse of antibiotics, irregular use of antibiotics, poor hospital infection control, and antibiotic residues in the environment have made the problem of bacterial resistance more serious. Bacterial resistance is a persistent and growing public health problem that has posed a serious threat to human health.

[0003] At present, the country has increased its control over the rational use of antibiotics, and the use of drug antibacterial concentration detection is of great significance to the rational use of antibiotics and the treatment of diseases. On the one hand, by measuring the antibacterial concentration of drugs in the patient's body, doctors can more accurately formulate treatment plans, more accurately determine the appropriate drug dosage and administration frequency, avoid excessive use of antibiotics, and reduce cross-infection transmission, effectively control the spread of bacteria and the spread of drug-resistant bacteria; on the other hand, when developing new antibacterial drugs, the antibacterial concentration can also be detected to help ensure that the drug has sufficient antibacterial activity, determine the appropriate dosage and administration schedule, and ultimately improve the efficacy and safety of new drugs.

[0004] In the prior art, the commonly used methods for detecting the antibacterial concentration of antimicrobial drugs mainly include the following four methods: broth dilution method, agar dilution method, E-test method and microdilution method. The above-mentioned methods for detecting the antibacterial concentration of drugs all require manual operation, and the results are determined by the naked eye, and real-time monitoring cannot be achieved. Not only is the work efficiency low, but also the objectivity is poor and the repeatability is low. Moreover, when studying colored natural drug monomers such as berberine, shikonin, rhein, baicalin, etc., and developing new traditional Chinese medicine preparations to treat drug-resistant bacterial infections, the color of the drug itself will seriously interfere with the determination results, resulting in the detection of the drug's antibacterial concentration being hindered. Summary of the invention

[0005] The purpose of the present invention is to provide a drug antibacterial concentration detection device to solve the technical problem that the drug antibacterial concentration detection methods in the prior art all require manual operation and the results are determined by the naked eye, and real-time monitoring cannot be achieved. Not only is the work efficiency low, but also the objectivity is poor and the repeatability is low. It is very easy for the color of the drug itself to seriously interfere with the determination result, resulting in obstruction of the drug antibacterial concentration detection.

[0006] To achieve the above-mentioned purpose, the technical solution adopted by the present invention is: to provide a drug antibacterial concentration detection device, comprising a casing with an inner cavity, a support frame arranged in the casing, and a rotating mechanism arranged on the support frame; the rotating mechanism comprises a accommodating turntable, a plurality of culture bottles placed on the accommodating turntable, and a driving motor arranged on the support frame and used to drive the accommodating turntable to rotate, the top surface of the accommodating turntable is evenly provided with a plurality of accommodating grooves for accommodating a plurality of the culture bottles along its circumferential side wall, and the accommodating turntable is provided with a heating component for heating the culture bottles; a clearance hole connecting the inner cavity and the outside is opened on the top of the casing, so that the culture bottles can be placed in or removed from the inner cavity, and the casing is provided with a sealing cover plate on the clearance hole; a detection component for detecting the bacterial concentration in the culture bottle is provided on the support frame.

[0007] In one embodiment, the detection component includes an infrared laser and a light source receiver compatible with the infrared laser, the support frame is provided with a fixing seat for mounting the detection component, the fixing seat is provided with a first fixing hole for mounting the infrared laser and a second fixing hole for mounting the light source receiver, the accommodating turntable is located in any of the accommodating slots and is provided with a first makeshift hole for the light source of the infrared laser to enter the accommodating slot and a second makeshift hole for the light source to scatter out of the accommodating slot, the first fixing hole and the first makeshift hole are connected, and the second fixing hole and the second makeshift hole are connected.

[0008] In one embodiment, the rotating mechanism further includes a rotating shaft fixedly connected to the accommodating turntable, and a synchronizing assembly for connecting the output shaft of the driving motor and the rotating shaft.

[0009] In one embodiment, the synchronization assembly includes a first synchronization wheel fixedly connected to the output shaft, a second synchronization wheel fixedly connected to the rotating shaft, and a synchronization belt wound between the first synchronization wheel and the second synchronization wheel.

[0010] In one embodiment, the drug antibacterial concentration detection device also includes a positioning component, which includes a photoelectric switch arranged on the support frame and a positioning turntable fixedly connected to the second synchronization wheel, the photoelectric switch and the positioning turntable are electrically connected, and the accommodating turntable is located in any of the accommodating grooves and is provided with a third yield hole for the light beam of the photoelectric switch to enter the accommodating groove.

[0011] In one embodiment, the drug antibacterial concentration detection device further includes a conductive slip ring sleeved on the rotating shaft, and a fixing frame for fixing and connecting the conductive slip ring is provided on the support frame.

[0012] In one embodiment, the drug antibacterial concentration detection device further includes a first magnetic attraction component disposed on the support frame and a second magnetic attraction component disposed in the culture bottle.

[0013] In one embodiment, the heating assembly includes a heating plate attached to the accommodating turntable, a temperature sensor fixedly connected to the accommodating turntable, and an over-temperature protection switch electrically connected to the temperature sensor and the heating plate.

[0014] In one embodiment, the drug antibacterial concentration detection device further includes a data processor disposed in the housing and a touch display screen disposed on the housing and electrically connected to the data processor.

[0015] The beneficial effects of the drug antibacterial concentration detection device provided by the present invention are as follows: compared with the prior art, the drug antibacterial concentration detection device of the present invention comprises a casing with an inner cavity, a support frame arranged in the casing, and a rotating mechanism arranged on the support frame, and the rotating mechanism is firmly installed in the casing through the support frame; a clearance hole connecting the inner cavity and the outside is opened on the top of the casing for the culture bottle to be placed in or removed from the inner cavity, and a sealing cover plate is provided on the casing on the clearance hole, which can prevent dust or debris from entering the inner cavity of the casing through the clearance hole when the drug antibacterial concentration detection device is in a standby state, which is beneficial to prolonging the service life of the drug antibacterial concentration detection device, and on the other hand, the culture bottle placed in the casing can be placed in a dark environment during the operation of the drug antibacterial concentration detection device, so as to avoid the external light source from affecting the normal operation of the drug antibacterial concentration detection device; the rotating mechanism comprises a accommodating turntable, a A plurality of culture bottles and a driving motor arranged on a support frame and used for driving a accommodating turntable to rotate; a plurality of accommodating grooves for accommodating a plurality of culture bottles are evenly arranged along the circumferential side wall of the top surface of the accommodating turntable; a heating component for heating the culture bottles is arranged on the accommodating turntable; a detection component for detecting the bacterial concentration in the culture bottles is arranged on the support frame; by placing a plurality of culture bottles in the plurality of accommodating grooves on the accommodating turntable, the heating component heats the accommodating turntable to a preset temperature to create a test environment for the culture bottles, and drives the accommodating turntable to rotate by the driving motor; and the detection component on the support frame is used to detect the bacterial concentration in the plurality of culture bottles, so as to realize real-time automatic monitoring of the inhibition of a certain bacterium by a plurality of drugs, or the sensitivity of a plurality of bacterial strains to the same drug, thereby avoiding the risk of interference with the determination result due to the color of the drug itself, effectively improving the efficiency and accuracy of drug antibacterial concentration detection, and being conducive to accelerating the research process of new antibacterial drugs.

[0016] The purpose of the present invention is also to provide a method for detecting the antibacterial concentration of a drug, so as to solve the technical problem that the antibacterial concentration detection methods of the drug in the prior art all require manual operation and the results are determined by the naked eye, and real-time monitoring cannot be achieved. Not only is the work efficiency low, but also the objectivity is poor and the repeatability is low. It is very easy for the color of the drug itself to seriously interfere with the determination result, resulting in obstruction of the drug antibacterial concentration detection.

[0017] To achieve the above object, the technical solution adopted by the present invention is: to provide a method for detecting the antibacterial concentration of a drug, which is implemented by using the above-mentioned antibacterial concentration detection device of the drug, and comprises the following steps:

[0018] S10: placing a plurality of culture bottles to be tested into the housing through the clearance holes on the housing of the drug antibacterial concentration detection device, placing the plurality of culture bottles to be tested one by one in the plurality of receiving grooves of the receiving turntable, and placing a sealing cover plate on the clearance holes of the housing;

[0019] S20: driving the motor to drive the accommodating turntable to rotate, and the photoelectric switch of the positioning component emits a light beam to the accommodating slot to determine whether the culture bottle to be tested is in the accommodating slot, and at the same time testing the normal operation of the detection component, and testing whether the first magnetic attraction component on the support frame repels the second magnetic attraction component in the culture bottle to be tested;

[0020] S30: the driving motor continuously drives the accommodating turntable to rotate, the heating component starts to operate, the heating plate heats the accommodating turntable, and the temperature sensor monitors the real-time temperature of the accommodating turntable in real time;

[0021] S40: When the temperature of the accommodating turntable rises to 10 degrees Celsius away from the preset temperature value, intermittent heating is used for heating, that is, the heating plate heats the accommodating turntable for a period of time, stops working for a period of time, and then heats again until the temperature of the accommodating turntable reaches the preset temperature value. The specific interval time can be customized;

[0022] S50: When the temperature of the accommodating turntable reaches a preset temperature value, the driving motor continuously drives the accommodating turntable to rotate, the photoelectric switch emits a light beam to the accommodating slot to determine whether there is a newly added culture bottle to be tested on the accommodating turntable, and the heating component heats the accommodating turntable in an intermittent heating manner to keep the temperature of the accommodating turntable constant at the preset temperature value;

[0023] S60: The detection component performs a bacterial concentration test on the culture bottle at a preset time interval. During each test, the infrared laser of the detection component starts the light source for 5 seconds and then takes a reading. The reading is taken once every 1 second, and the readings are taken continuously for n times (n is not less than 5). After removing the highest and lowest values ​​in the readings, the average value of the readings is taken, and a continuous plot is made with time as the horizontal axis and the average value of the readings as the vertical axis.

[0024] S70: When the detection is completed, the data processor generates an exportable chart and projects it on the touch screen, the drive motor continuously drives the receiving turntable to rotate, the photoelectric switch emits a light beam to the receiving slot to determine whether the culture bottle placed on the receiving turntable has been taken out, and the heating component heats the receiving turntable in an intermittent heating manner so that the temperature of the receiving turntable is kept constant at a preset temperature value.

[0025] The beneficial effect of the drug antibacterial concentration detection method provided by the present invention is that: compared with the prior art, the drug antibacterial concentration detection method of the present invention is implemented using the drug antibacterial concentration detection device as described above, by placing multiple culture bottles in multiple accommodating grooves on the accommodating turntable, the heating component heats the accommodating turntable to a preset temperature, creates a test environment for the culture bottles, and drives the accommodating turntable to rotate through a driving motor, and uses the detection component on the support frame to detect the bacterial concentration in multiple culture bottles, thereby realizing real-time automatic monitoring of the inhibition of multiple drugs on a certain bacterium, or the sensitivity of multiple strains of bacteria to the same drug, avoiding the risk of interference with the determination result due to the color of the drug itself, effectively improving the efficiency and accuracy of drug antibacterial concentration detection, and helping to accelerate the research process of new antibacterial drugs. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0027] Figure 1 Schematic diagram of the three-dimensional structure of the drug antibacterial concentration detection device provided in an embodiment of the present invention Figure 1 ;

[0028] Figure 2 Schematic diagram of the three-dimensional structure of the drug antibacterial concentration detection device provided in an embodiment of the present invention Figure 2 ;

[0029] Figure 3 A schematic diagram of the side view structure of a drug antibacterial concentration detection device provided in an embodiment of the present invention;

[0030] Figure 4 Schematic diagram of the three-dimensional structure of the drug antibacterial concentration detection device provided in an embodiment of the present invention Figure 3 ;

[0031] Figure 5 A schematic diagram of the three-dimensional structure of the accommodating turntable and the fixing seat of the drug antibacterial concentration detection device provided in an embodiment of the present invention;

[0032] Figure 6 A schematic diagram of the three-dimensional structure of a fixing base of a drug antibacterial concentration detection device provided in an embodiment of the present invention.

[0033] Among them, the reference numerals in the figure are:

[0034] 1. Casing; 11. Sealing cover; 12. Data processor; 13. Touch screen; 2. Support frame; 21. Fixing seat; 211. First fixing hole; 212. Second fixing hole; 22. Fixing frame; 3. Rotating mechanism; 31. Accommodating turntable; 311. Accommodating groove; 312. First clearance hole; 313. Second clearance hole; 314. Third clearance hole; 32. Culture bottle; 33. Driving motor; 34. Rotating shaft; 35. Synchronous component; 351. First synchronous wheel; 352. Second synchronous wheel; 353. Synchronous belt; 4. Heating component; 41. Heating plate; 42. Temperature sensor; 43. Over-temperature protection switch; 5. Detection component; 51. Infrared laser; 52. Light source receiver; 6. Photoelectric switch; 7. Positioning turntable; 8. Conductive slip ring; 9. First magnetic suction member. DETAILED DESCRIPTION

[0035] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0036] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it can be directly on the other element or indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element.

[0037] It should be understood that the orientation or position relationship indicated by terms such as "length", "width", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside" and "outside" are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0038] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0039] Please also read Figures 1 to 4The embodiment of the present invention provides a drug antibacterial concentration detection device, comprising a housing 1 with an inner cavity, a support frame 2 arranged in the housing 1, and a rotating mechanism 3 arranged on the support frame 2, wherein the rotating mechanism 3 is firmly installed in the housing 1 through the support frame 2; a clearance hole connecting the inner cavity and the outside is opened on the top of the housing 1, so that a culture bottle 32 can be placed in or removed from the inner cavity, and a sealing cover plate 11 is provided on the housing 1 at the clearance hole. On the one hand, it can prevent dust or debris from entering the inner cavity of the housing 1 through the clearance hole when the drug antibacterial concentration detection device is in a standby state, which is beneficial to prolonging the service life of the drug antibacterial concentration detection device; on the other hand, it can make the drug antibacterial concentration detection device in the operation process, the culture bottle 32 placed in the housing 1 is in a dark environment, and the external light source is prevented from affecting the normal operation of the drug antibacterial concentration detection device; the rotating mechanism 3 comprises a accommodating turntable 31, a plurality of culture bottles 32 placed on the accommodating turntable 31, and a sealing cover plate 11 arranged on the support frame 2 and used for A driving motor 33 is provided to drive the accommodating turntable 31 to rotate. A plurality of accommodating grooves 311 for accommodating a plurality of culture bottles 32 are evenly arranged on the top surface of the accommodating turntable 31 along its circumferential side wall. A heating component 4 for heating the culture bottles 32 is provided on the accommodating turntable 31. A detection component 5 for detecting the bacterial concentration in the culture bottles 32 is provided on the support frame 2. By placing a plurality of culture bottles 32 in the plurality of accommodating grooves 311 on the accommodating turntable 31, the heating component 4 heats the accommodating turntable 31 to a preset temperature to create a test environment for the culture bottles 32, and drives the accommodating turntable 31 to rotate by the driving motor 33. The bacterial concentration in the plurality of culture bottles 32 is detected by the detection component 5 on the support frame 2, so as to realize real-time automatic monitoring of the inhibition of a certain bacterium by a plurality of drugs, or the sensitivity of a plurality of bacterial strains to the same drug, thereby avoiding the risk of interference with the determination result due to the color of the drug itself, effectively improving the efficiency and accuracy of drug antibacterial concentration detection, and facilitating the research process of new antibacterial drugs.

[0040] Please also read Figure 1 to Figure 2 As a specific implementation of the drug antibacterial concentration detection device provided in this embodiment, the drug antibacterial concentration detection device also includes a data processor 12 arranged in the casing 1 and a touch screen 13 arranged on the casing 1 and electrically connected to the data processor 12. The data processor 12 collects real-time bacterial concentration data in multiple culture bottles 32 obtained by the detection component 5, processes and stores the detection result data, and generates an exportable chart about bacterial growth from the detection data, which is put on the touch screen 13 for users to read, which is convenient for data statistics and analysis, and provides convenience for research work; the casing 1 is provided with a touch screen 13, which is convenient for users to manually operate the drug antibacterial concentration detection device.

[0041] Optionally, the housing 1 is provided with a data transmission interface electrically connected to the data processor 12 , so that the user can extract and copy the detection data in the data processor 12 .

[0042] Please also read Figures 2 to 4 In this embodiment, the rotating mechanism 3 also includes a rotating shaft 34 fixedly connected to the accommodating turntable 31, and a synchronizing assembly 35 for connecting the output shaft of the driving motor 33 and the rotating shaft 34. By providing a synchronizing assembly 35 between the accommodating turntable 31 and the output shaft of the driving motor 33, and using the rotating shaft 34 for transmission, the kinetic energy output by the driving motor 33 is transmitted to the accommodating turntable 31. While driving the accommodating turntable 31 to rotate, the volume of the rotating mechanism 3 can be effectively reduced, the inner cavity space required to be occupied by the casing 1 can be reduced, and the overall volume of the drug antibacterial concentration detection device can be reduced.

[0043] Optionally, the synchronization component 35 includes a first synchronization wheel 351 fixedly connected to the output shaft, a second synchronization wheel 352 fixedly connected to the rotating shaft 34, and a synchronization belt 353 wound between the first synchronization wheel 351 and the second synchronization wheel 352. The output shaft of the driving motor 33 drives the first synchronization wheel 351 to rotate, and transfers kinetic energy to the second synchronization wheel 352 through the synchronization belt 353, so that the second synchronization wheel 352 rotates, and drives the rotating shaft 34 fixedly connected to the second synchronization wheel 352 to rotate, and then drives the accommodating turntable 31 fixedly connected to the rotating shaft 34 to rotate. The structure is simple and easy to assemble and produce.

[0044] Optionally, the drug antibacterial concentration detection device further comprises a conductive slip ring 8 sleeved on the rotating shaft 34, and a fixing frame 22 for fixing the conductive slip ring 8 is provided on the support frame 2. The conductive slip ring 8 is sleeved on the rotating shaft 34 to ensure the transmission of electric energy and signals, which is beneficial to simplifying the components inside the drug antibacterial concentration detection device. At the same time, the fixing frame 22 is used to firmly fix the conductive slip ring 8 at a preset position to prevent the conductive slip ring 8 from shifting due to vibration generated during operation of the drug antibacterial concentration detection device, which is beneficial to improving the structural stability and reliability of the drug antibacterial concentration detection device.

[0045] Please also read Figure 3 and Figure 4In this embodiment, the drug antibacterial concentration detection device also includes a positioning component, which includes a photoelectric switch 6 arranged on the support frame 2 and a positioning turntable 7 fixedly connected to the second synchronous wheel 352. The photoelectric switch 6 and the positioning turntable 7 are electrically connected. The accommodating turntable 31 is located in any accommodating groove 311 and is provided with a third yielding hole 314 for the light beam of the photoelectric switch 6 to enter the accommodating groove 311. The photoelectric sensing technology of the photoelectric switch 6 of the positioning component is used to monitor in real time whether a culture bottle 32 is placed in any accommodating groove 311 on the accommodating turntable 31, and the accommodating groove 311 where the culture bottle 32 is placed is positioned and marked by the positioning turntable 7 electrically connected to the photoelectric switch 6, and the bacterial concentration in the culture bottle 32 is monitored in real time by the detection component 5. When the test starts, the driving motor 33 drives the accommodating turntable 31 to rotate, and the photoelectric switch 6 of the positioning component emits a light beam to the third clearance hole 314 of the accommodating groove 311 to determine whether there is a culture bottle 32 to be tested in the accommodating groove 311; when the test is in progress, the driving motor 33 continues to drive the accommodating turntable 31 to rotate, and the photoelectric switch 6 emits a light beam to the third clearance hole 314 of the accommodating groove 311 to determine whether there is a newly added culture bottle 32 to be tested in the accommodating turntable 31; when the test ends, the driving motor 33 continues to drive the accommodating turntable 31 to rotate, and the photoelectric switch 6 emits a light beam to the third clearance hole 314 of the accommodating groove 311 to determine whether the culture bottle 32 placed on the accommodating turntable 31 has been taken out, or to determine whether there is a newly added culture bottle 32 to be tested in the accommodating turntable 31, so that the culture bottle 32 can be inspected as soon as it arrives without waiting, thereby effectively improving timeliness.

[0046] Please read further Figure 4 In this embodiment, the heating component 4 of the drug antibacterial concentration detection device includes a heating plate 41 attached to the accommodating turntable 31, a temperature sensor 42 fixedly connected to the accommodating turntable 31, and an over-temperature protection switch 43 electrically connected to the temperature sensor 42 and the heating plate 41. The accommodating turntable 31 is heated to a preset temperature by the heating plate 41 of the heating component 4 to create a test environment for the culture bottle 32, and the real-time temperature value of the accommodating turntable 31 is monitored by the temperature sensor 42 in real time; when the accommodating turntable 31 is heated to the preset temperature value, the over-temperature protection switch 43 is electrically connected to the temperature sensor 42 and the heating plate 41. The over-temperature protection switch 43 is disconnected, and the heating plate 41 stops working, preventing the temperature value of the accommodating turntable 31 from continuing to rise and exceeding the preset temperature value, causing the temperature in the culture bottle 32 to be too high, affecting the test results; when the temperature value of the accommodating turntable 31 is lower than the preset temperature value, the over-temperature protection switch 43 is closed, and the heating plate 41 starts working and heats the accommodating turntable 31 to the preset temperature value, so that the temperature value of the accommodating turntable 31 is constant at the preset temperature value, providing a constant temperature test environment for the culture bottle 32, which is conducive to ensuring the accuracy of the drug antibacterial concentration detection.

[0047] Please read further Figure 2In this embodiment, the drug antibacterial concentration detection device also includes a first magnetic member 9 arranged on the support frame 2 and a second magnetic member arranged in the culture bottle 32. The first magnetic member 9 arranged on the support frame 2 and the second magnetic member arranged in the culture bottle 32 are magnetically repelled by the principle of like magnetism repelling each other, so that the second magnetic member is subjected to the magnetic force and runs in the culture bottle 32, thereby playing a stirring role, which is beneficial to speed up the exchange of nutrients and metabolic wastes between bacteria and culture medium, not only promoting bacterial growth, but also preventing bacteria from adhering to the wall, agglomerating, precipitating, etc., which may cause deviations in the detection results, and effectively improving the repeatability and accuracy of the detection results.

[0048] Optionally, the second magnetic attraction member is a high temperature resistant magnetic rod to prevent the second magnetic attraction member from losing its magnetism due to the excessively high temperature in the culture bottle 32 .

[0049] Please also read Figure 2 , Figure 3 , Figure 5 and Figure 6 In this embodiment, the detection component 5 of the drug antibacterial concentration detection device includes an infrared laser 51 and a light source receiver 52 adapted to the infrared laser 51. A fixing seat 21 for mounting the detection component 5 is provided on the support frame 2. The detection component 5 is firmly mounted at a preset position by the fixing seat 21 provided on the support frame 2. A first fixing hole 211 for mounting the infrared laser 51 and a second fixing hole 212 for mounting the light source receiver 52 are provided on the fixing seat 21. The accommodating turntable 31 is provided with a first clearance hole 312 for the light source of the infrared laser 51 to enter the accommodating groove 311 and a second clearance hole 313 for the light source to scatter out of the accommodating groove 311 in any accommodating groove 311. The first fixing hole 211 and the first clearance hole 312 are connected, and the second fixing hole 212 and the second clearance hole 313 are connected. When the detection component 5 is started During operation, the infrared laser 51 emits a light source, which is emitted from the first fixing hole 211 of the fixing seat 21, passes through the first giving way hole 312 on the receiving turntable 31 and enters the receiving groove 311. The light source illuminates the solution in the culture bottle 32 and scatters. The scattered light source is emitted from the receiving groove 311, passes through the second giving way hole 313 on the receiving turntable 31 and enters the second fixing hole 212 on the fixing seat 21, and is received by the light source receiver 52. The turbidity of the solution in the culture bottle 32 is detected by the scattering turbidimetry principle, thereby realizing the detection of the bacterial concentration in multiple culture bottles 32, realizing real-time automatic monitoring of the inhibition of multiple drugs on a certain bacterium, or the sensitivity of multiple strains of bacteria to the same drug, avoiding the risk of interference with the determination result due to the color of the drug itself, effectively improving the efficiency and accuracy of drug antibacterial concentration detection, and facilitating the research process of new antibacterial drugs.

[0050] Optionally, the infrared laser 51 uses a 785nm infrared laser 51 as the incident light source.

[0051] Please also read Figures 1 to 6 The embodiment of the present invention also provides a method for detecting the antibacterial concentration of a drug. The method for detecting the antibacterial concentration of a drug is implemented using the above-mentioned antibacterial concentration detection device of the drug, and comprises the following steps:

[0052] S10: placing a plurality of culture bottles 32 to be tested into the housing 1 through the clearance holes on the housing 1 of the drug antibacterial concentration detection device, and placing the plurality of culture bottles 32 to be tested one by one in the plurality of receiving grooves 311 of the receiving turntable 31, and covering the sealing cover plate 11 on the clearance holes of the housing 1;

[0053] S20: The driving motor 33 drives the accommodating turntable 31 to rotate, and the photoelectric switch 6 of the positioning component emits a light beam to the accommodating groove 311 to determine whether there is a culture bottle 32 to be tested in the accommodating groove 311, and at the same time tests the normal operation of the detection component 5, and tests whether the first magnetic attraction member 9 on the support frame 2 and the second magnetic attraction member in the culture bottle 32 to be tested are of the same polarity and repel each other;

[0054] S30: the driving motor 33 continuously drives the accommodating turntable 31 to rotate, the heating component 4 starts to work, the heating plate 41 heats the accommodating turntable 31, and the temperature sensor 42 monitors the real-time temperature of the accommodating turntable 31 in real time;

[0055] S40: When the temperature of the accommodating turntable 31 rises to 10 degrees Celsius away from the preset temperature value, intermittent heating is used for heating, that is, the heating plate 41 heats the accommodating turntable 31 for a period of time, stops working for a period of time, and then heats again until the temperature of the accommodating turntable 31 reaches the preset temperature value. The specific interval time can be customized;

[0056] S50: When the temperature of the accommodating turntable 31 reaches the preset temperature value, the driving motor 33 continuously drives the accommodating turntable 31 to rotate, the photoelectric switch 6 emits a light beam to the accommodating groove 311 to determine whether there is a newly added culture bottle 32 to be tested on the accommodating turntable 31, and the heating component 4 heats the accommodating turntable 31 in an intermittent heating manner, so that the temperature of the accommodating turntable 31 is constant at the preset temperature value;

[0057] S60: The detection component 5 performs a bacterial concentration test on the culture bottle 32 at a preset time interval. During each test, the infrared laser 51 of the detection component 5 starts the light source and takes a reading 5 seconds later. The reading is taken once every 1 second, and the reading is taken n times in succession (n is not less than 5). After removing the highest and lowest values ​​in the readings, the average value of the readings is taken, and a continuous plot is made with time as the horizontal axis and the average value of the readings as the vertical axis.

[0058] S70: When the detection is completed, the data processor 12 generates an exportable chart and projects it on the touch screen 13, the driving motor 33 continuously drives the receiving turntable 31 to rotate, the photoelectric switch 6 emits a light beam to the receiving groove 311 to determine whether the culture bottle 32 placed on the receiving turntable 31 has been taken out, and the heating component 4 uses intermittent heating to heat the receiving turntable 31 so that the temperature of the receiving turntable 31 is kept constant at a preset temperature value.

[0059] The drug antibacterial concentration detection method provided by the present invention is implemented using the above-mentioned drug antibacterial concentration detection device. By placing multiple culture bottles 32 in multiple accommodating grooves 311 on the accommodating turntable 31, the heating component 4 heats the accommodating turntable 31 to a preset temperature to create a test environment for the culture bottles 32, and drives the accommodating turntable 31 to rotate through the driving motor 33. The detection component 5 on the support frame 2 is used to detect the bacterial concentration in the multiple culture bottles 32, thereby realizing real-time automatic monitoring of the inhibition of multiple drugs on a certain bacterium, or the sensitivity of multiple strains of bacteria to the same drug, avoiding the risk of interference with the determination result due to the color of the drug itself, effectively improving the efficiency and accuracy of drug antibacterial concentration detection, and helping to accelerate the research process of new antibacterial drugs.

[0060] The above description is only a preferred embodiment of the present embodiment and is not intended to limit the present embodiment. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present embodiment shall be included in the protection scope of the present embodiment.

Claims

1. A drug antibacterial concentration detection device, characterized in that: It includes a housing having an inner cavity, a support frame arranged in the housing, and a rotating mechanism arranged on the support frame; The rotating mechanism includes a accommodating turntable, a plurality of culture bottles placed on the accommodating turntable, and a driving motor arranged on the supporting frame and used for driving the accommodating turntable to rotate. The top surface of the accommodating turntable is evenly provided with a plurality of accommodating grooves for accommodating the plurality of culture bottles along its circumferential side wall, and a heating component for heating the culture bottles is arranged on the accommodating turntable; a clearance hole connecting the inner cavity and the outside is opened on the top of the casing for the culture bottles to be placed in or removed from the inner cavity, and a sealing cover plate is provided on the casing located on the clearance hole; a detection component for detecting the bacterial concentration in the culture bottle is arranged on the supporting frame.

2. The drug antibacterial concentration detection device according to claim 1, characterized in that: The detection component includes an infrared laser and a light source receiver compatible with the infrared laser. The support frame is provided with a fixing seat for mounting the detection component. The fixing seat is provided with a first fixing hole for mounting the infrared laser and a second fixing hole for mounting the light source receiver. The accommodating turntable is located in any of the accommodating slots and is provided with a first clearance hole for the light source of the infrared laser to be emitted into the accommodating slot and a second clearance hole for the light source to be scattered out of the accommodating slot. The first fixing hole and the first clearance hole are connected to each other, and the second fixing hole and the second clearance hole are connected to each other.

3. The drug antibacterial concentration detection device according to claim 1, characterized in that: The rotating mechanism further includes a rotating shaft fixedly connected to the accommodating rotating disk, and a synchronizing assembly for connecting the output shaft of the driving motor and the rotating shaft.

4. The drug antibacterial concentration detection device according to claim 3, characterized in that: The synchronous assembly includes a first synchronous wheel fixedly connected to the output shaft, a second synchronous wheel fixedly connected to the rotating shaft, and a synchronous belt wound between the first synchronous wheel and the second synchronous wheel.

5. The drug antibacterial concentration detection device according to claim 4, characterized in that: The drug antibacterial concentration detection device also includes a positioning component, which includes a photoelectric switch arranged on the support frame and a positioning turntable fixedly connected to the second synchronous wheel, the photoelectric switch and the positioning turntable are electrically connected, and the accommodating turntable is located in any of the accommodating grooves and is provided with a third yield hole for the light beam of the photoelectric switch to enter the accommodating groove.

6. The drug antibacterial concentration detection device according to claim 3, characterized in that: The drug antibacterial concentration detection device also includes a conductive slip ring sleeved on the rotating shaft, and a fixing frame for fixing and connecting the conductive slip ring is arranged on the supporting frame.

7. The drug antibacterial concentration detection device according to any one of claims 1 to 6, characterized in that: The drug antibacterial concentration detection device also includes a first magnetic attraction component arranged on the support frame and a second magnetic attraction component arranged in the culture bottle.

8. The drug antibacterial concentration detection device according to any one of claims 1 to 6, characterized in that: The heating assembly includes a heating plate attached to the accommodating turntable, a temperature sensor fixedly connected to the accommodating turntable, and an over-temperature protection switch electrically connected to the temperature sensor and the heating plate.

9. The drug antibacterial concentration detection device according to any one of claims 1 to 6, characterized in that: The drug antibacterial concentration detection device also includes a data processor arranged in the housing and a touch display screen arranged on the housing and electrically connected to the data processor.

10. A method for detecting the antibacterial concentration of a drug, the method being implemented by using the antibacterial concentration detection device of any one of claims 1 to 9, characterized in that: The following steps are involved: S10: placing a plurality of culture bottles to be tested into the housing through the clearance holes on the housing of the drug antibacterial concentration detection device, placing the plurality of culture bottles to be tested one by one in the plurality of receiving grooves of the receiving turntable, and placing a sealing cover plate on the clearance holes of the housing; S20: driving the motor to drive the accommodating turntable to rotate, and the photoelectric switch of the positioning component emits a light beam to the accommodating slot to determine whether the culture bottle to be tested is in the accommodating slot, and at the same time testing the normal operation of the detection component, and testing whether the first magnetic attraction component on the support frame repels the second magnetic attraction component in the culture bottle to be tested; S30: the driving motor continuously drives the accommodating turntable to rotate, the heating component starts to operate, the heating plate heats the accommodating turntable, and the temperature sensor monitors the real-time temperature of the accommodating turntable in real time; S40: When the temperature of the accommodating turntable rises to 10 degrees Celsius away from the preset temperature value, intermittent heating is used for heating, that is, the heating plate heats the accommodating turntable for a period of time, stops working for a period of time, and then heats again until the temperature of the accommodating turntable reaches the preset temperature value. The specific interval time can be customized; S50: When the temperature of the accommodating turntable reaches a preset temperature value, the driving motor continuously drives the accommodating turntable to rotate, the photoelectric switch emits a light beam to the accommodating slot to determine whether there is a newly added culture bottle to be tested on the accommodating turntable, and the heating component heats the accommodating turntable in an intermittent heating manner to keep the temperature of the accommodating turntable constant at the preset temperature value; S60: The detection component performs a bacterial concentration test on the culture bottle at a preset time interval. During each test, the infrared laser of the detection component starts the light source for 5 seconds and then takes a reading. The reading is taken once every 1 second, and the readings are taken continuously for n times (n is not less than 5). After removing the highest and lowest values ​​in the readings, the average value of the readings is taken, and a continuous plot is made with time as the horizontal axis and the average value of the readings as the vertical axis. S70: When the detection is completed, the data processor generates an exportable chart and projects it on the touch screen, the drive motor continuously drives the receiving turntable to rotate, the photoelectric switch emits a light beam to the receiving slot to determine whether the culture bottle placed on the receiving turntable has been taken out, and the heating component heats the receiving turntable in an intermittent heating manner so that the temperature of the receiving turntable is kept constant at a preset temperature value.

Images