Tremella fuciformis extract with moisture retention effect as well as preparation method and application of tremella fuciformis extract

Abstract

The invention relates to the technical field of plant extraction, and particularly discloses a tremella fuciformis extract with a water locking effect and a preparation method and application thereof. According to the application, the tremella sporocarp is obtained by cultivating autonomously separated tremella fungi, and then the tremella extract is prepared from the obtained tremella sporocarp in a high-temperature and high-pressure water extraction mode. Compared with an acid-base extraction method and an enzymolysis method, in the preparation process of the tremella fuciformis extract, an acid-base regulator is not added, and an enzyme which is poor in stability and needs to strictly control reaction conditions is not added; the extraction time is short, the extraction time can be greatly shortened, the extraction rate of tremella polysaccharide is also improved, and industrial production of the tremella extract is facilitated. Moreover, the prepared tremella fuciformis extract is rich in tremella fuciformis polysaccharide and various skin-care active substances, has good film-forming property, water locking property, short-time moisturizing property and oxidation resistance, and can promote the synthesis of hyaluronic acid of keratinocytes.

Description

Technical Field

[0001] The present application relates to the technical field of plant extraction, and in particular to a tremella extract with water-locking effect, and a preparation method and application thereof. Background Art

[0002] Tremella fuciformis Berk, also known as white fungus and snow fungus, has extremely high nutritional value and medicinal effects and is a health product recognized worldwide. Tremella is grown in Sichuan, Guizhou, Hubei, Shaanxi, Fujian, Zhejiang and other regions in my country.

[0003] Tremella fruiting bodies contain a variety of nutrients such as Tremella polysaccharides, proteins, trace elements, etc. Tremella polysaccharides have a variety of biological activities such as immunomodulation, hypoglycemic, hypolipidemic, and anti-tumor. High molecular weight Tremella polysaccharides have the characteristics of water absorption and water retention (generally refers to Tremella polysaccharides with a molecular weight of 1-1.5 million Da), and can be used as a plant-based moisturizer, and are gradually used in essences, facial masks, creams, lotions, skin care water and other cosmetics.

[0004] Common extraction methods for Tremella fuciformis extracts include enzymatic extraction and acid-base extraction. Among them, the enzymatic extraction method has the characteristics of poor thermal stability, easy inactivation, and limited pH; therefore, the extraction conditions need to be strictly controlled when extracting Tremella fuciformis extracts by enzymatic extraction, which is not conducive to the industrial production of Tremella fuciformis extracts. During the acid-base extraction process, improper control of the acid and alkali concentrations can easily destroy the stereo-optically active structure of Tremella fuciformis polysaccharides, leading to the degradation of polysaccharides, thereby affecting the extraction rate and biological activity of polysaccharides. In terms of cosmetic applications, the Tremella fuciformis extract content obtained by the above-mentioned extraction method is low, resulting in poor skin care effects. Summary of the invention

[0005] The purpose of the present application is to overcome the deficiencies of the above-mentioned prior art and to provide a tremella extract with water-locking effect, and a preparation method and application thereof. The method of the present application can improve the extraction rate of tremella polysaccharides and the extraction of other active ingredients, thereby improving the skin care effect of the tremella extract; the method of the present application shortens the extraction time of the tremella extract, and the extraction process is simple, which is suitable for industrial production; and the prepared tremella extract has low electrical conductivity, good film-forming properties, and has better water-locking, short-term moisturizing, anti-oxidation and other effects, and is suitable as a cosmetic raw material.

[0006] To achieve the above purpose, the technical solution adopted by this application is:

[0007] The present application provides a Tremella fungus, which is Tremella fuciformis JW-001, which has been deposited in the Guangdong Provincial Microbiological Culture Collection Center on July 19, 2024, with a deposit number of GDMCC No: 64891, a classification name of Tremella fuciformis, and a deposit address of 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou.

[0008] The present application provides a Tremella fruiting body, which is cultivated by using the Tremella fungus as described above.

[0009] Compared with commercially available Tremella fruiting bodies, the Tremella fruiting bodies obtained by cultivating the Tremella fungi isolated independently in the present application can retain high molecular weight Tremella polysaccharides to a greater extent, so that the final product has a better water-locking effect and thus a better short-term moisturizing effect.

[0010] The Tremella fungus independently isolated in the present application and the Tremella fruiting bodies obtained by cultivation can easily dissolve Tremella polysaccharides and other active substances under high temperature and high pressure conditions. The Tremella extract obtained in the present application has good water-locking, short-term moisturizing and anti-oxidation effects; and the Tremella extract of the present application can also promote the synthesis of hyaluronic acid in keratinocytes.

[0011] The present application provides a method for preparing a Tremella fuciformis extract having a water-locking effect, comprising the following steps:

[0012] 1) crushing the above-mentioned Tremella fuciformis fruiting bodies, sieving, and obtaining Tremella fuciformis powder;

[0013] 2) Evenly mixing the tremella powder and water, and extracting under high temperature and high pressure conditions to obtain a tremella extract with water-locking effect.

[0014] As a preferred embodiment of the method for preparing the Tremella fuciformis extract with water-locking effect described in the present application, the sieving mesh is a sieve with 150 to 250 meshes.

[0015] As a preferred embodiment of the method for preparing the tremella extract with water-locking effect described in the present application, the mass ratio of the tremella powder to water is 1: (80-120).

[0016] As a preferred embodiment of the preparation method of the Tremella fuciformis extract with water-locking effect described in the present application, the high temperature and high pressure conditions are: the extraction temperature is 110-130° C., and the extraction pressure is 0.15-0.3 Mpa;

[0017] The extraction time is 20 to 50 minutes.

[0018] The temperature and pressure of the extraction process have a great influence on the water-locking, short-term moisturizing and antioxidant effects of Tremella fuciformis extract; among them, temperature can accelerate the thermal motion between molecules and promote the dissolution of substances in Tremella fuciformis fruiting bodies in water; at the same time, high pressure can promote the release of intracellular substances in Tremella fuciformis fruiting bodies to the outside of the solvent, so that the final Tremella fuciformis extract has good water-locking effect, thereby improving the short-term moisturizing effect. At the same time, Tremella fuciformis extract also has a good antioxidant effect and can promote the synthesis of hyaluronic acid in keratinocytes.

[0019] As a preferred embodiment of the method for preparing the Tremella fuciformis extract with water-locking effect described in the present application, in the step 2), after the high-temperature and high-pressure extraction, a filtering step is also included, and the filtering method is plate and frame filter press filtration.

[0020] The present application uses a self-isolated Tremella fungus to cultivate Tremella fruiting bodies, and then obtains Tremella extract by high-temperature and high-pressure water extraction. Compared with the acid-base extraction method and the enzymatic hydrolysis method, the Tremella extract of the present application does not add an acid-base regulator or an enzyme with poor stability and requires strict control of reaction conditions during the preparation process; and the extraction time is short, which can greatly shorten the extraction time, and the extraction rate of Tremella polysaccharides is also improved, which is conducive to the industrial production of Tremella extract.

[0021] The tremella extract prepared in the present application is extracted from tremella fruiting bodies by means of high temperature and high pressure. Due to the relationship between the external pressure difference and the cellular pressure difference, the high pressure can promote the release of intracellular substances of the tremella fruiting bodies to the outside of the solvent, and the high temperature can accelerate the thermal motion of molecules. Under the conditions of high temperature and high pressure, a large amount of active substances in the tremella fruiting bodies are released into the solvent. The prepared tremella extract is rich in tremella polysaccharides and a variety of skin care active substances. The tremella extract has good film-forming, moisturizing and antioxidant properties.

[0022] The present application also provides a Tremella fuciformis extract prepared by the preparation method of the Tremella fuciformis extract.

[0023] The Tremella extract obtained by the above method has low conductivity, good film-forming property, moisturizing property, skin barrier repairing property and anti-oxidation effect. In addition, the Tremella extract of the present application can promote the synthesis of hyaluronic acid in keratinocytes.

[0024] The present application also provides the use of the Tremella fuciformis extract in the preparation of cosmetics.

[0025] The present application adopts a simple water extraction method to extract Tremella fuciformis fruiting bodies. No new reagents or materials are introduced during the extraction process. The prepared Tremella fuciformis extract has low conductivity (within 700us / cm), which fully meets the application requirements of different cosmetics such as essences, facial masks, and lotions for low conductivity of raw materials.

[0026] The cosmetics include one of essence, facial mask and lotion.

[0027] The selection of the above-mentioned cosmetics is not limited to the above-mentioned types, and can be applied to cosmetics containing any skin care functional substances.

[0028] The present application also provides a cosmetic, which contains the Tremella fuciformis extract, and the Tremella fuciformis extract is added in an amount of 0.1 to 50 wt % in the cosmetic.

[0029] In some specific embodiments, the cosmetic is a serum.

[0030] The essence comprises disodium EDTA, glycerin, carbomer, xanthan gum, p-hydroxyacetophenone, the above-mentioned tremella extract and water.

[0031] In some specific embodiments, the essence comprises the following components in percentage by mass: 0.03% disodium EDTA, 3% glycerol, 0.2% carbomer, 0.1% xanthan gum, 0.8% p-hydroxyacetophenone and 3% of the above-mentioned Tremella fuciformis extract, and the balance is water.

[0032] Adding Tremella extract to cosmetics can improve the moisturizing and anti-oxidation effects of cosmetics. Applying the prepared Tremella extract with low conductivity to cosmetics can also improve the stability of cosmetics.

[0033] Compared with the prior art, this application has the following beneficial effects:

[0034] The present application provides a tremella extract with water-locking effect and its preparation method and application. The present application adopts the self-isolated Tremella fungus to cultivate the tremella fruiting body, and then the obtained tremella fruiting body is subjected to high temperature and high pressure water extraction to prepare the tremella extract. Compared with the acid-base extraction method and the enzymatic hydrolysis method, the tremella extract of the present application does not add an acid-base regulator during the preparation process, and does not add an enzyme with poor stability and strict control of reaction conditions; and the extraction time is short, which can greatly shorten the extraction time, and the extraction rate of tremella polysaccharide is also improved, which is conducive to the industrial production of tremella extract. In addition, the prepared tremella extract prepared by the present application is rich in tremella polysaccharides and a variety of skin care active substances. The tremella extract has good film-forming properties, water lock, short-term moisturizing properties and antioxidant properties, and can also promote the synthesis of hyaluronic acid (HA) of keratinocytes. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 This is a morphological diagram of Tremella fungi;

[0036] Figure 2 Schematic diagram of the water locking performance of Example 3 and Comparative Example 1. DETAILED DESCRIPTION

[0037] In order to better illustrate the purpose, technical solutions and advantages of the present application, the present application will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0038] In the following examples and comparative examples, the experimental methods used are conventional methods unless otherwise specified, and the materials, reagents, etc. used are all commercially available unless otherwise specified, and the components and raw materials used in each parallel experiment are of the same type.

[0039] Unless otherwise specified, all percentages mentioned in this application are by mass percentages.

[0040] Embodiment 1, the separation of Tremella fungus

[0041] The Tremella fungus of the present application comes from Tongjiang County, Sichuan Province. The Tremella fungus mycelium is isolated from dead wood where wild Tremella grows, and the Tremella fungus species is prepared.

[0042] The specific separation and preparation method of Tremella fungus strains are as follows: take the dead wood where wild Tremella grows, cut the dead wood with Tongjiang wild Tremella into 1 cm thick wood chips, cut off the part without sterile hyphae, and set aside. Use the flame of an alcohol lamp to sterilize the surface of the wood chips. Place the sterilized wood chips on sterile gauze, use tools to cut the wood chips into small pieces as big as matches, place the small pieces on PDA culture medium, and culture at 25°C. Check the PDA culture medium for contamination by foreign bacteria every 3 days, and immediately eliminate the culture medium if foreign bacteria are found. On the 10th day of culture, short velvety developmental hyphae (i.e., Tremella fungus hyphae) appeared on the small wood particles of some PDA culture media, and light yellow water droplets gradually formed around the hyphae. Pick up such developmental hyphae, inoculate them in PDA solid culture medium, and culture them at 25°C for 14 days to obtain purified Tremella fungus. Pictures of Tremella fungus strains are shown in the following figure. Figure 1 As shown, the Tremella fungus strains were transferred to a refrigerated storage at 4°C for later use.

[0043] The Tremella fungus screened above was preserved and named Tremellafuciformis JW-001. It was deposited in Guangdong Provincial Microbiological Culture Collection Center on July 19, 2024. Its preservation number is GDMCC No: 64891, and its classification name is Tremella fuciformis. The preservation address is: 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou.

[0044] The formula of PDA culture medium is: 200 g potato (peeled), 20 g glucose, 18-20 g agar, and 1000 mL water.

[0045] The preparation method of PDA culture medium is as follows: boil potatoes and 1100 mL of water for 15 minutes, filter with gauze, make up the filtrate to 1000 mL with water, add agar and glucose, mix well, sterilize at 121°C for 15 minutes, and set aside after cooling.

[0046] Embodiment 2, the cultivation of Tremella fruiting body

[0047] (1) Preparation of Tremella fuciformis fruiting body cultivars: 78% sawdust, 20% bran, 1% gypsum, and 1% white sugar are uniformly stirred in a mixer, and deionized water is added while stirring to make the water content of the stirred culture medium between 55% and 60% to obtain a mixture. The mixture is placed in a fungus bag, sterilized at 100° C. for 18 hours, and cooled to obtain a seed-carrying culture medium.

[0048] Under sterile conditions, holes were punched on the seed culture medium with a punch rod (sterilized), with the punching parameters being 1.5 cm in diameter, 1.5 cm in depth, and 10 cm in spacing between holes. The Tremella fungus species of Example 1 were inoculated into the holes with an inoculation scoop to obtain Tremella fruiting body cultivars.

[0049] (2) Inoculation culture: The Tremella fuciformis fruiting bodies were cultivated at 25° C. and a relative humidity of 80% for 20 days to obtain Tremella fuciformis fruiting bodies.

[0050] (3) Post-treatment: The harvested Tremella fuciformis fruiting bodies are dried at 40° C. to obtain dried Tremella fuciformis fruiting bodies.

[0051] Example 3: A Tremella fuciformis extract having water-locking effect

[0052] The preparation method of the Tremella extract of this embodiment is:

[0053] (1) The dried Tremella fuciformis fruiting bodies obtained by cultivation in Example 2 were crushed and passed through a 200-mesh sieve, and the Tremella fuciformis powder of the lower layer was removed for later use.

[0054] (2) The Tremella powder is mixed evenly with 100 times the mass of water, extracted at 115° C. and 0.2 MPa for 30 min, and the extracted mixture is filtered through a plate and frame filter press to obtain a filtrate as the Tremella extract.

[0055] Embodiments 4 to 7: A Tremella extract with water-locking effect

[0056] The preparation methods of the Tremella fuciformis extracts with water-locking effect of Examples 4 to 7 are similar to those of Example 3, with only some parameters in step (2) being different from those of Example 3, and the remaining steps being the same as those of Example 3. The differences between Examples 4 to 7 and Example 3 are shown in Table 1.

[0057] Table 1

[0058] Group Extraction temperature / ℃ Extraction pressure / Mpa Example 3 115 0.2 Example 4 110 0.2 Example 5 130 0.2 Example 6 115 0.15 Example 7 115 0.3

[0059] Example 8: A Tremella fuciformis extract with water-locking effect

[0060] The preparation method of the Tremella extract of this embodiment is:

[0061] (1) The dried Tremella fuciformis fruiting bodies obtained by cultivation in Example 2 were crushed and passed through a 150-mesh sieve, and the Tremella fuciformis powder of the lower layer was removed for later use.

[0062] (2) The Tremella powder was mixed evenly with 80 times the mass of water, and extracted at 120° C. and 0.20 MPa for 20 min. The extracted mixture was filtered through a plate and frame filter press to obtain a filtrate as the Tremella extract.

[0063] Example 9: A Tremella fuciformis extract having water-locking effect

[0064] The preparation method of the Tremella extract of this embodiment is:

[0065] (1) The dried Tremella fuciformis fruiting bodies obtained by cultivation in Example 2 were crushed and passed through a 250-mesh sieve, and the Tremella fuciformis powder of the lower layer was removed for later use.

[0066] (2) The Tremella powder was mixed evenly with 120 times the mass of water, and extracted at 125° C. and 0.25 MPa for 50 min. The extracted mixture was filtered through a plate and frame filter press, and the obtained filtrate was the Tremella extract.

[0067] Comparative Example 1: A Tremella extract

[0068] The Tremella fuciformis extract was prepared using commercially available common Tremella fuciformis fruiting bodies (dried). The preparation method of the Tremella fuciformis extract was the same as that in Example 3.

[0069] Comparative Example 2: A Tremella Extract

[0070] The extraction temperature in Example 3 was changed from 115° C. to 80° C., and the remaining steps and parameters remained unchanged to prepare the Tremella fuciformis extract.

[0071] Comparative Example 3: Tremella fuciformis extract

[0072] The extraction pressure in Example 3 was changed from 0.2 MPa to extraction under ambient pressure and in boiling water (water temperature was 100° C.), during which the water was refluxed using a condenser, and the remaining steps and parameters remained unchanged to prepare the Tremella fuciformis extract.

[0073] Comparative Example 4: Tremella fuciformis extract (acid hydrolysis method)

[0074] (1) The dried Tremella fuciformis fruiting bodies obtained by cultivation in Example 2 were crushed and passed through a 200-mesh sieve, and the Tremella fuciformis powder of the lower layer was removed for later use.

[0075] (2) The Tremella powder was mixed evenly with 100 times the mass of citric acid solution (the concentration of citric acid was 0.1 mol / L), and extracted in boiling water (water temperature was 100° C.) at ambient pressure for 30 min, during which the water was refluxed using a condenser, and the extracted mixture was filtered through a plate and frame filter press to obtain a filtrate as the Tremella extract.

[0076] Comparative Example 5: A Tremella Extract (Acid Hydrolysis)

[0077] (1) The dried Tremella fuciformis fruiting bodies obtained by cultivation in Example 2 were crushed and passed through a 200-mesh sieve, and the Tremella fuciformis powder of the lower layer was removed for later use.

[0078] (2) The Tremella powder was mixed evenly with 100 times the mass of a citric acid solution (the concentration of the citric acid was 0.1 mol / L), and extracted at 115° C. and 0.2 MPa for 30 min. The extracted mixture was filtered through a plate and frame filter press, and the obtained filtrate was the Tremella extract.

[0079] Comparative Example 6: Tremella fuciformis extract (enzymatic hydrolysis)

[0080] (1) The dried Tremella fuciformis fruiting bodies obtained by cultivation in Example 2 were crushed and passed through a 200-mesh sieve, and the Tremella fuciformis powder of the lower layer was removed for later use.

[0081] (2) Mix the Tremella powder and 100 times the mass of water evenly, add a complex enzyme (cellulase: pectinase: xylanase = 1:1:1) with a mass ratio of 0.5% of the Tremella powder, perform enzymolysis at 55° C. for 30 min, filter the extracted mixture through a plate and frame filter press, and the obtained filtrate is the Tremella extract.

[0082] Test Example 1: Conductivity Test

[0083] Test samples: Tremella fuciformis extracts of Examples 3-9 and Comparative Examples 1-6.

[0084] Test steps: Use a conductivity meter to test the conductivity of the sample. Repeat the test 3 times for each sample and take the average value of the test results. The test results are shown in Table 2.

[0085] Table 2

[0086] Group Conductivity(us / cm) Group Conductivity(us / cm) Example 3 520 Comparative Example 1 1045 Example 4 501 Comparative Example 2 482 Example 5 549 Comparative Example 3 538 Example 6 608 Comparative Example 4 2622 Example 7 512 Comparative Example 5 3250 Example 8 622 Comparative Example 6 1291 Example 9 593 / /

[0087] The test results show that the conductivity of the Tremella fuciformis extract prepared by the preparation method of the embodiment of the present application is low, and the Tremella fuciformis extract obtained from the commercially available Tongjiang Tremella fuciformis fruiting body (Comparative Example 1) has a higher conductivity, which is not as good as the Tremella fuciformis extract obtained by independently isolating the strain and culturing the Tremella fuciformis fruiting body of the present invention.

[0088] The Tremella extracts prepared by the acid hydrolysis method (Comparative Examples 4 and 5) and the enzymatic hydrolysis method (Comparative Example 6) have high electrical conductivity, which can easily lead to system instability when used in cosmetic formulations.

[0089] Test Example 2: Water-locking performance test

[0090] Test samples: Tremella fuciformis extracts of Examples 3-9 and Comparative Examples 1-6.

[0091] Test steps: Apply 8g of the test sample evenly on the filter paper and dry it at 80℃ for 1h; pass 20g of water containing a color indicator through the filter paper containing the test sample, record the amount of water passing through after 2h, and judge the water-locking effect of the test sample based on the amount of water passing through. The smaller the amount of water passing through, the better the water-locking effect of the test sample. Repeat the test 3 times for each test sample, and take the average value of the test results.

[0092] The test process of the Tremella fuciformis extract obtained in Example 3 and Comparative Example 1 is as follows: Figure 2 shown.

[0093] The test results are shown in Table 3 below.

[0094] Table 3

[0095] Group Water throughput (g) Group Water throughput (g) Example 3 2.8 Comparative Example 1 9.3 Example 4 4.0 Comparative Example 2 6.5 Example 5 3.2 Comparative Example 3 7.8 Example 6 3.6 Comparative Example 4 12.7 Example 7 2.7 Comparative Example 5 18.5 Example 8 3.0 Comparative Example 6 15.1 Example 9 3.5 / /

[0096] The test results show that the water throughput of Examples 3 to 9 of the present application is less than that of Comparative Examples 1 to 5, indicating that the Tremella extracts of the Examples of the present application have better water-locking effects than those of the Comparative Examples.

[0097] The water-locking effect of Example 3 is better than that of Comparative Example 1, indicating that compared with the commercially available Tremella fruiting bodies, the Tremella extract obtained by independently cultivating Tremella fruiting bodies in the present application has a better water-locking effect. The water-locking effect of Example 3 is better than that of Comparative Examples 2 and 3, indicating that in the present application, the temperature and extraction pressure of the extraction process have a great influence on the water-locking effect of the Tremella extract. In the present application, the temperature can accelerate the thermal motion between molecules and promote the dissolution of the substances of the Tremella fruiting bodies in water; at the same time, the high pressure can promote the release of the intracellular substances of the Tremella fruiting bodies to the outside of the solvent, so that the Tremella extract finally obtained has a good water-locking effect.

[0098] The water-locking effect of Example 3 is better than that of Comparative Examples 4 to 6, indicating that compared with the traditional acid-base extraction method and enzymatic hydrolysis method, the high temperature and high pressure extract of the present application can retain high molecular weight Tremella polysaccharides and other active ingredients to a greater extent, making the final product have a better water-locking effect.

[0099] Test Example 3: Short-term moisturizing effect test

[0100] Test samples: moisturizing essences prepared in Examples 3 to 9 and Comparative Examples 1 to 6, and blank essences.

[0101] The formula of the moisturizing essence is shown in Table 4 below:

[0102] Table 4

[0103]

[0104] The preparation method of the moisturizing essence is as follows: add the materials of phase A into the main pot, heat to 85°C after homogenizing for 5 minutes, stir and mix evenly, and keep warm for 20 minutes; cool to 50°C, add the materials of phase B, stir and mix evenly; cool to 40°C, add the materials of phase C, stir and mix evenly to obtain the moisturizing essence.

[0105] The formula of the blank essence is similar to that of the moisturizing essence, except that component C is replaced with deionized water of equal mass; the preparation method of the blank essence is the same as that of the moisturizing essence.

[0106] Test steps: 140 healthy volunteers with facial skin stratum corneum moisture content below 20 C.U. were selected, and 10 volunteers each used one essence, for a total of 14 groups. The facial skin stratum corneum moisture content was tested before using the sample and 1h and 3h after using the sample. The moisturizing effect of the essence was judged based on the difference in facial skin stratum corneum moisture content before and after using the sample.

[0107] Test instrument and software: Skin moisture tester CM825, Courage&Khazaka, Germany.

[0108] The test results are shown in Table 5 below.

[0109] Table 5

[0110]

[0111]

[0112] The test results show that the short-term moisturizing effect of the Tremella extract of the examples of the present application is better than that of the Tremella extract of the comparative example. Among them, the water-locking effect of Example 3 is better than that of Comparative Example 1, indicating that compared with the Tremella extract prepared from the commercially available Tremella fruiting body, the Tremella extract obtained by the Tremella fruiting body independently separated and cultivated by the present application has a better short-term moisturizing effect.

[0113] The short-term moisturizing effect of Example 3 is better than that of Comparative Examples 2 and 3, indicating that in the present application, the temperature and pressure of the extraction process have a great influence on the short-term moisturizing effect of the Tremella fuciformis extract. In the present application, the temperature can accelerate the thermal motion between molecules and promote the dissolution of the substances in the Tremella fuciformis fruiting body in water; at the same time, the high pressure can promote the release of the intracellular substances in the Tremella fuciformis fruiting body to the outside of the solvent, so that the Tremella fuciformis extract finally obtained has a good short-term moisturizing effect.

[0114] The short-term moisturizing effect of Example 3 is better than that of Comparative Examples 4 to 6, indicating that compared with the traditional acid-base extraction method and enzymatic hydrolysis method, the Tremella fruiting body prepared by the Tremella fungus independently isolated in the present application can retain high-molecular-weight Tremella polysaccharides to a greater extent, so that the final product has a better water-locking effect, and thus a better short-term moisturizing effect.

[0115] Test Example 4: Antioxidant Performance Test

[0116] Test principle: The antioxidant properties of the Tremella extracts were determined by testing the scavenging rates of the Tremella extracts of Examples 3 to 9 and Comparative Examples 1 to 6 on hydroxyl radicals (·OH). The higher the scavenging rate of the Tremella extract on hydroxyl radicals (·OH), the better the antioxidant properties of the Tremella extract.

[0117] Test samples: The Tremella fuciformis extracts of Examples 3 to 9 and Comparative Examples 1 to 6 were prepared into 3% and 5% aqueous solutions with deionized water and stored in brown bottles.

[0118] Test steps: Take 2.0mL of sample solution of different concentrations, add 0.5mL of 6mmol / L FeSO4 solution and 0.5mL of 6mmol / L H2O2 solution in turn, mix thoroughly and react at room temperature for 10min, then add 0.5mL of 6mmol / L salicylic acid and mix, react at room temperature for 30min, and measure the absorbance at 510nm as A1. In the above reaction system, anhydrous ethanol is used instead of salicylic acid, and the absorbance is measured as A2, and distilled water is used instead of sample solution, and the absorbance is measured as A0. The OH removal rate is calculated according to the following formula:

[0119]

[0120] The average value of three measurements is taken, and the test results are shown in Table 6 below.

[0121] Table 6

[0122] concentration 3％ 5％ Example 3 46.8 68.2 Example 4 41.9 65.6 Example 5 47.0 70.1 Example 6 40.5 62.6 Example 7 47.0 68.0 Example 8 38.4 63.7 Example 9 36.6 60.4 Comparative Example 1 6.2 18.3 Comparative Example 2 22.5 37.9 Comparative Example 3 21.8 41.5 Comparative Example 4 15.2 21.2 Comparative Example 5 14.1 28.6 Comparative Example 6 18.6 30.7

[0123] The test results show that the antioxidant effect of the Tremella extract of the embodiment of the present application is better than that of the comparative example. Among them, the antioxidant effect of Example 3 is better than that of Comparative Example 1, indicating that compared with the commercially available Tremella fruiting bodies, the Tremella extract obtained by independently separating strains and cultivating Tremella fruiting bodies in the present application has a better antioxidant effect, and the extract of Tremella fruiting bodies on the market cannot achieve the antioxidant effect of the present application. The antioxidant effect of Example 3 is better than that of Comparative Examples 2 and 3, indicating that in the present application, the temperature and extraction pressure of the extraction process have a great influence on the antioxidant effect of the Tremella extract. In the present application, the temperature can accelerate the thermal motion between molecules and promote the dissolution of the active substances of the Tremella fruiting bodies in water; at the same time, the high pressure can promote the release of intracellular substances of the Tremella fruiting bodies to the outside of the solvent, so that the Tremella extract finally obtained has a good antioxidant effect. The antioxidant effect of Example 3 is better than that of Comparative Examples 4 to 6, indicating that compared with the traditional acid-base extraction method and enzymatic hydrolysis method, the high temperature and high pressure extract of the present application has a better antioxidant effect.

[0124] Test Example 5: Hyaluronic Acid Content Test

[0125] Test principle: Hyaluronic acid (HA), also known as hyaluronic acid, is a disaccharide unit glycosaminoglycan composed of D-glucuronic acid and N-acetylglucosamine. Hyaluronic acid, together with proteins such as collagen and elastin, constitute the extracellular matrix. Hyaluronic acid has excellent water retention capacity, so it is believed that it helps to moisturize the skin and maintain skin elasticity, and helps to reduce skin wrinkles. Therefore, this test uses an ELISA kit to detect changes in the HA content in the supernatant of keratinocytes, so as to evaluate whether the sample has the effect of moisturizing, promoting skin elasticity, and reducing wrinkles.

[0126] Test sample: The Tremella fuciformis extracts of Examples 3 to 9 and Comparative Examples 1 to 6 were prepared into a 1% solution using MEM culture medium containing 10% fetal bovine serum.

[0127] Test steps: Human keratinocytes (HaCaT) in the logarithmic phase were cultured at 1×10 5 The cells were inoculated at a concentration of 10 cells / mL in a 96-well culture plate, with 6 parallel wells in each group, and 100 μL of MEM culture medium containing 10% fetal bovine serum (referred to as culture medium) was added, and cultured at 5% CO2 and 37°C for 24 hours; the culture medium was aspirated and discarded; 100 μL of culture medium was added to the blank control group, and 100 μL of the test sample was added to the sample group, and cultured at 5% CO2 and 37°C for 48 hours; the culture medium was aspirated and discarded, 100 μL of culture medium was added to each well, and the supernatant was collected after culturing at 5% CO2 and 37°C for 24 hours, and the HA content was determined by an ELISA kit, and the HA content of the blank control group was taken as 100%, and the relative content of HA in the samples of the remaining groups was calculated.

[0128] The test results are shown in Table 7 below.

[0129] Table 7

[0130]

[0131]

[0132] The test results show that the Tremella extracts of the examples of the present application can promote HA synthesis in keratinocytes, and the effect is better than that of the comparative example. The effect of promoting HA (hyaluronic acid) synthesis in Example 3 is better than that of Comparative Example 1, indicating that compared with the commercially available Tremella fruiting bodies, the Tremella extracts of Tremella fruiting bodies obtained by independently separating strains and cultivating the Tremella fruiting bodies of the present application can better promote cell synthesis of HA, and the commercially available Tremella fruiting body extracts cannot achieve the effect of the present application.

[0133] The effect of promoting HA synthesis in Example 3 is better than that in Comparative Examples 2 and 3, indicating that in the present application, the temperature and extraction pressure of the extraction process have a great influence on the promotion of HA synthesis of Tremella fuciformis extract. In the present application, temperature can accelerate the thermal motion between molecules and promote the dissolution of active substances in Tremella fuciformis fruiting bodies in water; at the same time, high pressure can promote the release of intracellular substances in Tremella fuciformis fruiting bodies to the outside of the solvent, so that the Tremella fuciformis extract finally obtained has a good effect of promoting HA synthesis. The effect of promoting HA synthesis in Example 3 is worse than that in Comparative Examples 4 to 6, and Comparative Examples 4 and 5 have almost no effect of promoting HA synthesis, indicating that the effect of promoting HA synthesis of Tremella fuciformis extracts prepared by acid hydrolysis and enzymatic hydrolysis is not as good as that of high-pressure extraction.

[0134] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present application rather than to limit the scope of protection of the present application. Although the present application has been described in detail with reference to the preferred embodiments, ordinary technicians in this field should understand that the technical solution of the present application can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present application.

Claims

1. A Tremella fungus, characterized in that The Tremella fungus is Tremella fuciformis JW-001, which was deposited in the Guangdong Provincial Microbiological Culture Collection Center on July 19, 2024, with the collection number GDMCC No: 64891, and the collection address: 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou.

2. A Tremella fruiting body, characterized in that The Tremella fruiting body is cultivated by using the Tremella fungus as claimed in claim 1.

3. A method for preparing a Tremella fuciformis extract having a water-locking effect, characterized in that: The following steps are involved: 1) crushing the Tremella fruiting body as claimed in claim 2, sieving, and obtaining Tremella powder; 2) Evenly mixing the tremella powder and water, and extracting under high temperature and high pressure conditions to obtain a tremella extract with water-locking effect.

4. The method for preparing the Tremella fuciformis extract according to claim 3, wherein The sieve used for screening is a sieve with a mesh size of 150 to 250.

5. The method for preparing the Tremella fuciformis extract according to claim 3, wherein The mass ratio of the tremella powder to water is 1:(80-120).

6. The method for preparing the Tremella fuciformis extract according to claim 3, wherein The high temperature and high pressure conditions are: extraction temperature is 110-130°C, extraction pressure is 0.15-0.3Mpa; The extraction time is 20 to 50 minutes.

7. The method for preparing the Tremella fuciformis extract according to claim 3, wherein In the step 2), after the high temperature and high pressure extraction, a filtering step is also included, and the filtering method is plate and frame filter press filtering.

8. The Tremella fuciformis extract prepared by the method for preparing the Tremella fuciformis extract according to any one of claims 3 to 7.

9. Use of the Tremella fuciformis extract as claimed in claim 8 in preparing cosmetics.

10. A cosmetic, characterized in that: The cosmetic contains the Tremella fuciformis extract according to claim 8, and the Tremella fuciformis extract is added to the cosmetic in an amount of 0.1 to 50 wt%.

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