Mycoplasma synoviae drug sensitivity detection assembly and test method thereof

Abstract

The invention discloses a mycoplasma synoviae drug sensitivity detection assembly capable of ensuring test accuracy and reducing test cost and a test method thereof. The mycoplasma synoviae drug sensitivity detection assembly comprises a drug sensitivity plate, a mycoplasma synoviae culture medium freeze-dried powder bottle and paraffin oil, a phenol red indicator is mixed in mycoplasma synoviae culture medium freeze-dried powder, two rows of test holes are formed in the drug sensitive plate, and a negative test hole, a positive test hole and eight low-concentration antibiotic test holes are formed in one row of test holes. A negative test hole, a positive test hole and eight high-concentration antibiotic test holes are formed in the other row of test holes, each low-concentration antibiotic test hole and one high-concentration antibiotic test hole are aligned one by one to form a group, a detachable transparent sealing cover is arranged on the drug sensitive plate, and the drug sensitive plate is placed in an aluminum foil bag to be vacuumized and stored.

Description

Technical Field

[0001] The invention relates to the technical field of microbial detection, and in particular to a Mycoplasma synoviae drug sensitivity detection component and a testing method thereof. Background Art

[0002] In recent years, with the improvement of people's living standards, the market demand for eggs and chicken has been increasing, which has promoted the large-scale development of the domestic poultry industry. The large-scale and intensive chicken farming model has greatly improved the economic benefits of chicken farmers, but also caused the spread of bacterial diseases. In the process of visiting the market, it was found that the main cause of poultry disease is mycoplasma infection. At present, 28 types of mycoplasmas have been isolated and identified in poultry. Only two of them are highly pathogenic to chickens, one is Mycoplasma gallisepticum and the other is Mycoplasma synoviae. Mycoplasma gallisepticum was discovered a long time ago, and now there are some specific drugs for treatment. Mycoplasma synoviae disease is a disease that has become popular in chickens in the past two years. The common response measures for Mycoplasma synoviae disease on the market are either to use antibiotics for treatment, but different chicken farms have different feeding methods. Different types of antibiotics will be added to prevent diseases, so chickens in different chicken farms have different resistance to different types of antibiotics when they are suffering from synoviae mycoplasma disease. If antibiotics are abused, pathogens will develop resistance, and eventually there will be no cure. Moreover, when humans eat such chicken, it will affect their health. Either Chinese medicine is used for prevention, but Chinese medicine can only improve the disease resistance of chickens and cannot kill pathogens. Or the chickens are vaccinated, but vaccination not only cannot target chickens with vertical transmission, but chickens are also prone to stress reactions when vaccinated, affecting their growth and egg production. Summary of the invention

[0003] The object of the present invention is to provide a Mycoplasma synoviae drug sensitivity detection component and a testing method thereof, which are easy to operate and can quickly detect antibiotics with high sensitivity to Mycoplasma synoviae in a short time.

[0004] To achieve the above-mentioned purpose, the technical scheme adopted by the present invention is: a synoviae mycoplasma drug sensitivity detection component, comprising: a drug sensitivity plate, a synoviae mycoplasma culture medium freeze-dried powder bottle and paraffin oil, wherein the synoviae mycoplasma culture medium freeze-dried powder is mixed with a phenol red indicator, two rows of test holes are arranged on the drug sensitivity plate, one row of test holes is provided with negative test holes, positive test holes and eight low-concentration antibiotic test holes, and the other row of test holes is provided with negative test holes, positive test holes and eight high-concentration antibiotic test holes, each low-concentration antibiotic test hole and a high-concentration antibiotic test hole are aligned one by one to form a group, and tylosin, tylosin, tylosin, tilmicosin, norfloxacin, oxytetracycline, doxycycline, tylosin and levofloxacin are placed in each group of relatively aligned low-concentration antibiotic test holes and high-concentration antibiotic test holes, respectively, a detachable transparent sealing cover is provided on the drug sensitivity plate, and the drug sensitivity plate is placed in an aluminum foil bag for vacuum storage.

[0005] Further, the aforementioned Mycoplasma synoviae drug sensitivity detection component, wherein the concentrations of tylosin in the low-concentration antibiotic test hole and the high-concentration antibiotic test hole for tylosin are 5 mg / L and 25 mg / L respectively; the concentrations of tylosin in the low-concentration antibiotic test hole and the high-concentration antibiotic test hole for tylosin are 2 mg / L and 10 mg / L respectively; the concentrations of tylosin in the low-concentration antibiotic test hole and the high-concentration antibiotic test hole for tilmicosin are 10 mg / L and 50 mg / L respectively; the concentrations of norfloxacin in the low-concentration antibiotic test hole and the high-concentration antibiotic test hole for norfloxacin are 10 mg / L and 50 mg / L respectively; the concentrations of oxytetracycline in the low-concentration antibiotic test hole and the high-concentration antibiotic test hole for oxytetracycline are 10 mg / L and 50 mg / L respectively. The concentration of oxytetracycline in the test hole and the high-concentration antibiotic test hole is 5 mg / L and 20 mg / L respectively; the concentration of doxycycline in the low-concentration antibiotic test hole and the high-concentration antibiotic test hole for doxycycline is 2 mg / L and 10 mg / L respectively; the concentration of tylosin in the low-concentration antibiotic test hole and the high-concentration antibiotic test hole for tylosin is 2 mg / L and 10 mg / L respectively; the concentration of tylosin in the low-concentration antibiotic test hole and the high-concentration antibiotic test hole for levofloxacin is 2 mg / L and 10 mg / L respectively. Antibiotics of different concentrations and types are attached to the bottom inner walls of the corresponding low-concentration antibiotic test holes and high-concentration antibiotic test holes by using an antibiotic drying process.

[0006] Furthermore, in the aforementioned Mycoplasma synoviae drug sensitivity detection component, labels corresponding to the negative test holes, the positive test holes, and each group of aligned low-concentration antibiotic test holes and high-concentration antibiotic test holes are provided on the drug sensitivity plate.

[0007] The testing method of the Mycoplasma synoviae drug sensitivity detection assembly comprises the following steps:

[0008] S1. Open the bottle of freeze-dried powder of Mycoplasma synoviae culture medium, absorb sterile pure water and add it to the bottle of freeze-dried powder of Mycoplasma synoviae culture medium to dissolve the freeze-dried powder of Mycoplasma synoviae culture medium to form a culture medium solution;

[0009] S2. Take the drug sensitivity plate out of the aluminum foil bag, place it on the inoculation table, remove the transparent sealing cover, draw the culture medium solution treated with S1 and drip it into the two negative test wells, then inoculate the mycoplasma culture in the remaining culture medium solution and mix well;

[0010] S3, using a sterile nozzle to drip the culture medium treated by S2 into the positive test well, the low-concentration antibiotic test well and the high-concentration antibiotic test well in sequence, the volume of the culture medium dripped into the negative test well, the positive test well, the low-concentration antibiotic test well and the high-concentration antibiotic test well is the same, then drip a drop of paraffin oil into the negative test well, the positive test well, the low-concentration antibiotic test well and the high-concentration antibiotic test well, and cover with a transparent sealing cover;

[0011] S4. Place the drug sensitivity plate at 37°C, and when the culture medium in the positive test well turns yellow and the culture medium in the negative test well remains red, read the test results;

[0012] S5. Observe the color change of the culture medium in each antibiotic test well, and judge the drug sensitivity of Mycoplasma synoviae to each antibiotic according to the color of the culture medium in each antibiotic test well. If the culture medium remains red, it means that the mycoplasma in the culture medium is inhibited or killed. If the culture medium turns yellow, it means that the mycoplasma in the culture medium is growing.

[0013] Furthermore, in the aforementioned test method of the Mycoplasma synoviae drug sensitivity detection assembly, in S2 and S3, the volume of the culture medium dripped into the negative test well, the positive test well, the low-concentration antibiotic test well, and the high-concentration antibiotic test well is 150 μL.

[0014] Further, in the aforementioned test method for the drug sensitivity detection assembly of synoviae Mycoplasma, the volume of the culture medium solution in S1 is 4 mL, 200 μL of mycoplasma culture is inoculated in S2, and the number of mycoplasmas detected in the mycoplasma culture is not less than 10 5 ccu / ml.

[0015] Further, in the aforementioned testing method of the synoviae drug sensitivity detection assembly, in S5, when the culture medium in the low-concentration antibiotic test well and the high-concentration antibiotic test well are both red, it indicates that the synoviae Mycoplasma has a high sensitivity to the antibiotic; when the culture medium in the low-concentration antibiotic test well is yellow and the culture medium in the high-concentration antibiotic test well is red, it indicates that the synoviae Mycoplasma has a medium sensitivity to the antibiotic; when the culture medium in the low-concentration antibiotic test well and the high-concentration antibiotic test well are both yellow, it indicates that the synoviae Mycoplasma is resistant to the antibiotic.

[0016] Further, in the aforementioned test method of the synovia bursa mycoplasma drug sensitivity detection assembly, in S4, if the culture medium in the positive test well is all red after 24 hours, and the culture medium in all low-concentration antibiotic test wells and high-concentration antibiotic test wells is also red, it indicates that the mycoplasma culture in the culture medium is inactivated and dead; if the culture medium in the positive test well is all red after 24 hours, and the culture medium in the low-concentration antibiotic test well and the high-concentration antibiotic test well presents a corresponding drug sensitivity reaction, it indicates that the mycoplasma culture in the culture medium is active, but the culture medium and the mycoplasma culture are not mixed, and there is no mycoplasma culture in the culture medium dripped into the positive test well.

[0017] Furthermore, in the aforementioned testing method of the Mycoplasma synoviae drug sensitivity detection assembly, during the testing process, 2 to 3 sets of Mycoplasma synoviae drug sensitivity detection assemblies need to be used for simultaneous testing.

[0018] The advantages of the present invention are: using the drug sensitivity test to determine the drug sensitivity of synoviae Mycoplasma, so as to accurately and effectively use the corresponding antibiotic drugs for treatment, enhance the targeted use of drugs, and reduce the generation of drug-resistant strains; the test results do not require the use of any testing equipment, and the test results can be simply and clearly judged according to the color changes of the culture medium in each test well of the drug sensitivity plate in about 24 hours, so that antibiotics with high drug sensitivity to synoviae Mycoplasma can be quickly selected, which saves time for treatment and reduces the economic losses caused by the death of livestock and poultry, while ensuring the accuracy of the test and reducing the test cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the structure of the drug sensitivity plate in the Mycoplasma synoviae drug sensitivity detection assembly described in the present invention when viewed from above.

[0020] Figure 2 yes Figure 1 Schematic diagram of the structure in the main viewing direction.

[0021] Figure 3 It is a schematic diagram showing different drug sensitivities on the drug sensitivity plate when the test is valid.

[0022] Figure 4 It is a schematic diagram of the interpretation of the results after the culture medium in the negative test well of the drug sensitivity plate is contaminated.

[0023] Figure 5 It is a schematic diagram of the interpretation of the results after the culture medium in any high-concentration antibiotic test well on the drug sensitivity plate is contaminated.

[0024] Figure 6 It is a schematic diagram of the result interpretation after the mycoplasma culture in the culture medium of all the test wells in the drug sensitivity plate is inactivated and dead.

[0025] Figure 7 It is a schematic diagram of the result interpretation after the culture medium and mycoplasma culture in the positive test well of the drug sensitivity plate are not mixed.

[0026] in, Figure 3 to Figure 7 The culture medium colors in the low-concentration antibiotic test wells and high-concentration antibiotic test wells involved are only for the convenience of explanation and are not the actual test results. DETAILED DESCRIPTION

[0027] The technical solution of the present invention is further described below in conjunction with the accompanying drawings and preferred embodiments.

[0028] The Mycoplasma synoviae drug sensitivity detection assembly of the present invention comprises: a drug sensitivity plate 1, a Mycoplasma synoviae culture medium freeze-dried powder bottle and paraffin oil, wherein the Mycoplasma synoviae culture medium freeze-dried powder is mixed with a phenol red indicator, the Mycoplasma synoviae culture medium freeze-dried powder is prepared by purchasing a basic powder from outside, configuring it into a solution, adding phenol red, serum and coenzyme, adjusting the pH, and finally freeze-drying it, and the paraffin oil is purchased from outside, such as Figure 1 , Figure 2As shown, two rows of test holes are arranged on the drug sensitivity plate 1, one row of test holes is provided with negative test holes 11, positive test holes 12 and eight low-concentration antibiotic test holes 13, and the other row of test holes is provided with negative test holes 11, positive test holes 12 and eight high-concentration antibiotic test holes 14, each low-concentration antibiotic test hole 13 and a high-concentration antibiotic test hole 14 are aligned one by one to form a group, and tylosin, tylosin, tylosin, tilmicosin, norfloxacin, oxytetracycline, doxycycline, tylosin and lycomycin are placed in each group of relatively aligned low-concentration antibiotic test holes 13 and high-concentration antibiotic test holes 14, respectively, and a detachable transparent sealing cover 15 is provided on the drug sensitivity plate 1, and the drug sensitivity plate 1 is placed The drug-sensitive plate 1 is vacuum-stored in an aluminum foil bag, and is provided with labels corresponding to the negative test holes 11, the positive test holes 12, and each group of aligned low-concentration antibiotic test holes 13 and high-concentration antibiotic test holes 14, wherein the negative test hole 11 is labeled "-", the positive test hole 12 is labeled "+", the low-concentration antibiotic test holes 13 and the high-concentration antibiotic test holes 14 aligned to place tylosin are labeled "Tyl", and the concentrations of tylosin in the low-concentration antibiotic test holes 13 and the high-concentration antibiotic test holes 14 where tylosin is placed are 5 mg / L and 25 mg / L, respectively; ... aligned to place tylosin are 5 mg / L and 25 mg / L, respectively. The antibiotic test hole 14 is marked as "Val", and the concentrations of tylosin in the low-concentration antibiotic test hole 13 and the high-concentration antibiotic test hole 14 for tylosin are 2 mg / L and 10 mg / L respectively; the aligned low-concentration antibiotic test hole 13 and the high-concentration antibiotic test hole 14 for tilmicosin are marked as "Til", and the concentrations of tylosin in the low-concentration antibiotic test hole 13 and the high-concentration antibiotic test hole 14 for tilmicosin are 10 mg / L and 50 mg / L respectively; the aligned low-concentration antibiotic test hole 13 and the high-concentration antibiotic test hole 14 for norfloxacin are marked as "Nor", and the low-concentration antibiotic test hole 13 and the high-concentration antibiotic test hole 14 for norfloxacin are marked as "Nor". 3 and the high-concentration antibiotic test well 14, the concentrations of norfloxacin are 10 mg / L and 50 mg / L respectively; the aligned low-concentration antibiotic test well 13 and the high-concentration antibiotic test well 14 for placing oxytetracycline are marked as "Oxy", and the concentrations of oxytetracycline in the low-concentration antibiotic test well 13 and the high-concentration antibiotic test well 14 for placing oxytetracycline are 5 mg / L and 20 mg / L respectively; the aligned low-concentration antibiotic test well 13 and the high-concentration antibiotic test well 14 for placing doxycycline are marked as "Dox", and the concentrations of doxycycline in the low-concentration antibiotic test well 13 and the high-concentration antibiotic test well 14 for placing doxycycline are 2 mg / L and 10 mg / L respectively;The aligned low-concentration antibiotic test wells 13 and high-concentration antibiotic test wells 14 for placing tiamulin are marked as "Tia", and the concentrations of tiamulin in the low-concentration antibiotic test wells 13 and high-concentration antibiotic test wells 14 for placing ligomycin are 2 mg / L and 10 mg / L respectively; the aligned low-concentration antibiotic test wells 13 and high-concentration antibiotic test wells 14 for placing ligomycin are marked as "Lin-Spe", and the concentrations of ligomycin in the low-concentration antibiotic test wells 13 and high-concentration antibiotic test wells 14 for placing ligomycin are 2 mg / L and 10 mg / L respectively. Since different types of antibiotics have differences in action mechanism, antibacterial spectrum and stability, different concentrations need to be selected according to specific antibiotics, and then antibiotics of different concentrations and different types are attached to the bottom inner wall of the corresponding low-concentration antibiotic test wells 13 and high-concentration antibiotic test wells 14 by using the antibiotic drying process. ;

[0029] The test method of the Mycoplasma synoviae drug sensitivity test kit comprises the following steps:

[0030] S1. Open the bottle of freeze-dried powder of Mycoplasma synoviae culture medium, absorb sterile pure water and add it to the bottle of freeze-dried powder of Mycoplasma synoviae culture medium to dissolve the freeze-dried powder of Mycoplasma synoviae culture medium to form a culture medium solution. The volume of the culture medium solution in this embodiment is 4 mL. Since the freeze-dried powder of Mycoplasma synoviae culture medium is mixed with phenol red indicator, the phenol red indicator is red under alkaline conditions, and the pH of the culture medium is alkaline, so the culture medium solution is red;

[0031] S2. Observe whether the aluminum foil bag of the drug sensitivity plate is damaged or leaking. If it is damaged or leaking, it needs to be discarded. After the aluminum foil bag is not damaged or leaking, take the drug sensitivity plate out of the aluminum foil bag, place it on the inoculation table, remove the transparent sealing cover, and draw the culture medium solution in S1 and drip it into the two negative test holes. The amount drawn is 150 μL, and then inoculate 200 μL of mycoplasma culture in the remaining culture medium solution. The number of mycoplasmas detected in the mycoplasma culture should not be less than 10 5 ccu / ml, mixed evenly, the mycoplasma culture is obtained by collecting the joint cavity contents or other potentially infected tissue samples from sick chickens and then centrifuging to remove impurities, and finally using the clear liquid and culture medium for subsequent inoculation. In this step, 300 μL of culture medium is taken out of the original 4 mL culture medium, and 3.7 mL of culture medium is left, and then 200 μL of mycoplasma culture is inoculated (the number of mycoplasmas detected in the mycoplasma culture is not less than 10 5 ccu / ml), the inoculation volume of mycoplasma culture accounts for about 5.4% of the total volume of the culture medium. At this ratio, it is closer to simulating the actual situation of mycoplasma infection in animals, and at the same time, a relatively high mycoplasma growth density is obtained, thereby improving the credibility and timeliness of the experimental results;

[0032] S3, drip the culture medium treated by S2 into the positive test well, the low-concentration antibiotic test well and the high-concentration antibiotic test well in turn, the volume of the culture medium dripped into the positive test well, the low-concentration antibiotic test well and the high-concentration antibiotic test well is 150 μL, then drip a drop of paraffin oil into the negative test well, the positive test well, the low-concentration antibiotic test well and the high-concentration antibiotic test well to prevent the solution from volatilizing and cross-infection, and cover with a transparent sealing cover;

[0033] In this step, since the volume of the culture medium in each antibiotic test well is 150 μL, the weight of the corresponding antibiotic in each antibiotic test well can be determined according to the volume of the culture medium and the required concentration of the antibiotic when producing the drug sensitivity plate. Taking tylosin as an example, it is necessary to ensure that the concentrations of tylosin in the low-concentration antibiotic test well and the high-concentration antibiotic test well where tylosin is placed are 5 mg / L and 25 mg / L, respectively, that is, 5 mg / L and 25 mg / L tylosin stock solutions are prepared respectively, and when producing the drug sensitivity plate, 150 μL of 5 mg / L tylosin stock solution is added to the low-concentration antibiotic test well, and 150 μL of 25 mg / L tylosin stock solution is added to the high-concentration antibiotic test well, and then the corresponding weight of antibiotics is attached to the corresponding antibiotic test wells by a drying process;

[0034] S4, place the drug sensitivity plate treated in S3 at 37°C. Due to the difference in growth time of different pathogenic materials, the test results can only be interpreted after the culture medium in the positive test well turns yellow completely. In this step, if the culture medium in the positive test well does not turn yellow after 24 hours, it means that the mycoplasma culture has died, the test result is inaccurate, and it needs to be discarded and retested;

[0035] S5. Since mycoplasma will ferment glucose when growing, and the acidic substances produced by fermenting glucose will reduce the pH to acidic, and the phenol red indicator in the culture medium will change from red to yellow after encountering acid, observe the color change of the culture medium in each antibiotic test well, and judge the drug sensitivity of synoviae Mycoplasma to each antibiotic according to the color of the culture medium in each antibiotic test well. If the culture medium remains red, it means that the mycoplasma in the culture medium is inhibited or killed, and if the culture medium turns yellow, it means that the mycoplasma in the culture medium is growing;

[0036] In this step, if Figure 3As shown, when the culture medium in the two negative test wells is red and the culture medium in the two positive test wells is yellow, the test is valid. When the culture medium in the low-concentration antibiotic test well and the high-concentration antibiotic test well are both red, it indicates that Mycoplasma synoviae is highly sensitive to the antibiotic; when the culture medium in the low-concentration antibiotic test well is yellow and the culture medium in the high-concentration antibiotic test well is red, it indicates that Mycoplasma synoviae is moderately sensitive to the antibiotic; when the culture medium in the low-concentration antibiotic test well and the high-concentration antibiotic test well are both yellow, it indicates that Mycoplasma synoviae is resistant to the antibiotic.

[0037] During the test, if Figure 4 When the culture medium in the negative test well turns yellow, it means that the drug sensitivity plate is contaminated; Figure 5 If the culture medium in any low-concentration antibiotic test well shown in the figure is red and the culture medium in the high-concentration antibiotic test well aligned with it turns yellow, it also indicates that the drug sensitivity plate is contaminated; Figure 6 When the culture medium in all the test wells shown is red, it means that the mycoplasma culture dropped into the culture medium is inactivated and dead; Figure 7 When the culture medium in the positive test hole remains red, it means that the culture medium and the mycoplasma culture are not mixed evenly, and there is no mycoplasma culture in the culture medium dropped into the positive test hole. When any of the above situations occurs, it means that the test result is invalid, and a new synovia bursa mycoplasma drug sensitivity detection component needs to be replaced and retested. Retesting will extend the test cycle and affect the treatment and medication time. Therefore, during the test process, 2 to 3 sets of synovia bursa mycoplasma drug sensitivity detection components are generally used at the same time. In this way, even if one set of synovia bursa mycoplasma drug sensitivity detection components is contaminated with drug sensitivity plates, mycoplasma culture is dead or not mixed evenly during the test, other drug sensitivity plates can also provide accurate test results and will not affect the subsequent treatment and medication time.

[0038] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, ordinary technicians in the relevant field should understand that the specific implementation methods of the present invention can still be modified or replaced by equivalents. Any modification or equivalent replacement that does not depart from the spirit and scope of the present invention should be covered within the scope of protection of the claims of the present invention.

Claims

1. Mycoplasma synoviae drug sensitivity test kit, include: A drug sensitivity plate, a bottle of freeze-dried powder of mycoplasma synoviae culture medium and paraffin oil, characterized in that: phenol red indicator is mixed in freeze-dried powder of mycoplasma synoviae culture medium, two rows of test holes are arranged on the drug sensitivity plate, one row of test holes is provided with negative test holes, positive test holes and eight low-concentration antibiotic test holes, and the other row of test holes is provided with negative test holes, positive test holes and eight high-concentration antibiotic test holes, each low-concentration antibiotic test hole and a high-concentration antibiotic test hole are aligned one by one to form a group, tylosin, tylosin, tylosin, tilmicosin, norfloxacin, oxytetracycline, doxycycline, tylosin and levofloxacin are placed in each group of relatively aligned low-concentration antibiotic test holes and high-concentration antibiotic test holes, respectively, a detachable transparent sealing cover is provided on the drug sensitivity plate, and the drug sensitivity plate is placed in an aluminum foil bag for vacuum storage.

2. The Mycoplasma synoviae drug sensitivity detection assembly according to claim 1, Features: The concentrations of tylosin in the low-concentration antibiotic test well and the high-concentration antibiotic test well for tylosin are 5 mg / L and 25 mg / L respectively; the concentrations of tylosin in the low-concentration antibiotic test well and the high-concentration antibiotic test well for tylosin are 2 mg / L and 10 mg / L respectively; the concentrations of tylosin in the low-concentration antibiotic test well and the high-concentration antibiotic test well for tilmicosin are 10 mg / L and 50 mg / L respectively; the concentrations of norfloxacin in the low-concentration antibiotic test well and the high-concentration antibiotic test well for norfloxacin are 10 mg / L and 50 mg / L respectively; the low-concentration antibiotic test well and the high-concentration antibiotic test well for oxytetracycline are The concentrations of oxytetracycline in the low-concentration antibiotic test wells and high-concentration antibiotic test wells are 5 mg / L and 20 mg / L respectively; the concentrations of doxycycline in the low-concentration antibiotic test wells and high-concentration antibiotic test wells are 2 mg / L and 10 mg / L respectively; the concentrations of tylosin in the low-concentration antibiotic test wells and high-concentration antibiotic test wells are 2 mg / L and 10 mg / L respectively; the concentrations of tylosin in the low-concentration antibiotic test wells and high-concentration antibiotic test wells are 2 mg / L and 10 mg / L respectively; antibiotics of different concentrations and types are attached to the bottom inner walls of the corresponding low-concentration antibiotic test wells and high-concentration antibiotic test wells by using an antibiotic drying process.

3. The Mycoplasma synoviae drug sensitivity detection assembly according to claim 1, Features: The drug sensitivity plate is provided with labels corresponding to the negative test holes, the positive test holes, and each group of aligned low-concentration antibiotic test holes and high-concentration antibiotic test holes.

4. The testing method of the Mycoplasma synoviae drug sensitivity detection assembly according to any one of claims 1 to 3, Features: The following steps are involved: S1. Open the bottle of freeze-dried powder of Mycoplasma synoviae culture medium, absorb sterile pure water and add it to the bottle of freeze-dried powder of Mycoplasma synoviae culture medium to dissolve the freeze-dried powder of Mycoplasma synoviae culture medium to form a culture medium solution; S2. Take the drug sensitivity plate out of the aluminum foil bag, place it on the inoculation table, remove the transparent sealing cover, draw the culture medium solution treated with S1 and drip it into the two negative test wells, then inoculate the mycoplasma culture in the remaining culture medium solution and mix well; S3, dripping the culture medium treated by S2 into the positive test well, the low-concentration antibiotic test well and the high-concentration antibiotic test well in sequence, the volume of the culture medium dripped into the negative test well, the positive test well, the low-concentration antibiotic test well and the high-concentration antibiotic test well is the same, then dripping a drop of paraffin oil into the negative test well, the positive test well, the low-concentration antibiotic test well and the high-concentration antibiotic test well, and covering with a transparent sealing cover; S4. Place the drug sensitivity plate at 37°C, and when the culture medium in the positive test well turns yellow and the culture medium in the negative test well remains red, read the test results; S5. Observe the color change of the culture medium in each antibiotic test well, and judge the drug sensitivity of Mycoplasma synoviae to each antibiotic according to the color of the culture medium in each antibiotic test well. If the culture medium remains red, it means that the mycoplasma in the culture medium is inhibited or killed. If the culture medium turns yellow, it means that the mycoplasma in the culture medium is growing.

5. The testing method of the Mycoplasma synoviae drug sensitivity detection assembly according to claim 3, Features: In S2 and S3, the volume of the culture medium dripped into the negative test well, the positive test well, the low-concentration antibiotic test well, and the high-concentration antibiotic test well was 150 μL.

6. The testing method of the Mycoplasma synoviae drug sensitivity detection assembly according to claim 3, Features: In S1, the volume of the culture medium solution is 4 mL, 200 μL of mycoplasma culture is inoculated in S2, and the number of mycoplasmas detected in the mycoplasma culture is not less than 10 5 ccu / ml.

7. The testing method of the Mycoplasma synoviae drug sensitivity detection assembly according to claim 3, Features: In S5, when the culture media in the low-concentration antibiotic test well and the high-concentration antibiotic test well are both red, it indicates that Mycoplasma synoviae is highly sensitive to the antibiotic; When the culture medium in the low-concentration antibiotic test well is yellow and the culture medium in the high-concentration antibiotic test well is red, it indicates that Mycoplasma synoviae has moderate sensitivity to the antibiotic; when the culture medium in both the low-concentration antibiotic test well and the high-concentration antibiotic test well is yellow, it indicates that Mycoplasma synoviae is resistant to the antibiotic.

8. The testing method of the Mycoplasma synoviae drug sensitivity detection assembly according to claim 3, Features: In S4, if the culture medium in the positive test wells is all red after 24 hours, and the culture medium in all low-concentration antibiotic test wells and high-concentration antibiotic test wells is also red, it means that the mycoplasma culture in the culture medium is inactivated and dead; if the culture medium in the positive test wells is all red after 24 hours, and the culture medium in the low-concentration antibiotic test wells and the high-concentration antibiotic test wells shows corresponding drug sensitivity reactions, it means that the mycoplasma culture in the culture medium is active, but the culture medium and the mycoplasma culture are not mixed, and there is no mycoplasma culture in the culture medium dropped into the positive test well.

9. The testing method of the Mycoplasma synoviae drug sensitivity detection assembly according to claim 3, Features: During the testing process, 2 to 3 sets of Mycoplasma synoviae drug sensitivity detection kits are required to perform testing simultaneously.

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