Sterilization composition and application thereof, sterilization preparation and preparation method and application thereof

Abstract

The invention relates to the technical field of agricultural pest control, in particular to a bactericidal composition and application thereof, a bactericidal preparation and a preparation method and application thereof. The bactericidal composition comprises validamycin and dithianon in a mass ratio of (1-40): (1-40). The bactericidal composition is used for preventing and treating wheat scab, the prevention and treatment effect can be improved, the pesticide use amount and the pesticide use cost are reduced, and the occurrence of resistance is retarded.

Description

Technical Field

[0001] The present invention relates to the technical field of agricultural pest control, and in particular to a bactericidal composition and application thereof, a bactericidal preparation and a preparation method and application thereof. Background Art

[0002] Wheat is one of the three major grains. As of 2021, my country has 23,570,000 hectares of wheat plantations, which are mainly divided into five dominant production areas, namely the Huanghuaihai wheat dominant area, the middle and lower reaches of the Yangtze River wheat dominant area, the southwest wheat dominant area, the northwest wheat dominant area, and the northeast wheat dominant area. Fusarium head blight, rust, powdery mildew, sheath blight, leaf blight, and root rot are important fungal diseases that threaten wheat production. Among them, wheat head blight can cause a 30% to 50% reduction in wheat production. The pathogen of this disease, Fusarium graminearum, can produce deoxynivalenol (DON) toxins, which contaminate crop fruits and grain products, and have a wide range of toxic effects on humans and animals, affecting the health of humans and livestock.

[0003] Chemical control is an important means of controlling wheat fusarium rust. However, commonly used chemicals such as carbendazim and methyl thiophanate have basically lost their value in controlling fusarium rust.

[0004] In view of this, the present invention is proposed. Summary of the invention

[0005] The first purpose of the present invention is to provide a fungicide composition, which can reduce the conventional amount of pesticides used to prevent and control wheat fusarium rust, solve the problem that when validamycin and dicyanoanthraquinone are used alone, the prevention and control of wheat fusarium rust is not ideal. At the same time, the fungicide composition can reduce the resistance of wheat fusarium rust to validamycin and dicyanoanthraquinone.

[0006] The second object of the present invention is to provide a bactericidal preparation.

[0007] The third object of the present invention is to provide a method for preparing the bactericidal preparation as described above.

[0008] The fourth object of the present invention is to provide use of the fungicidal composition or the fungicidal preparation as described above in preventing and controlling fungal diseases of crops.

[0009] In order to achieve the above-mentioned purpose of the present invention, the following technical solutions are particularly adopted:

[0010] The invention provides a bactericidal composition, which comprises validamycin and dicyanoanthraquinone in a mass ratio of 1-40:1-40.

[0011] The present invention also provides a bactericidal preparation, comprising the bactericidal composition as described above, and auxiliary materials.

[0012] The present invention further provides a method for preparing the bactericidal preparation as described above, comprising the following steps:

[0013] Mix the bactericidal composition and auxiliary materials evenly.

[0014] The present invention further provides use of the fungicidal composition or the fungicidal preparation as described above in preventing and controlling fungal diseases of crops.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] The bactericidal composition provided by the present invention has different action mechanisms of validamycin and dicyanoanthraquinone, and the two are mixed to have obvious synergistic effect, and has a certain inhibitory effect on wheat fusarium graminearum, which can improve the disease prevention effect, reduce the amount of medicine used, and slow down the generation of resistance. DETAILED DESCRIPTION

[0017] The technical scheme of the present invention will be clearly and completely described below in conjunction with specific embodiments, but it will be appreciated by those skilled in the art that the following described embodiments are part of embodiments of the present invention, rather than all embodiments, and are only used to illustrate the present invention, and should not be considered as limiting the scope of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative work, all belong to the scope of protection of the present invention. If specific conditions are not indicated in the embodiments, they are carried out according to normal conditions or conditions recommended by the manufacturer. If the manufacturer is not indicated in the reagents or instruments used, they are all conventional products that can be purchased commercially.

[0018] In a first aspect, the present invention provides a fungicide composition, specifically a fungicide composition that can be used to prevent and treat wheat fusarium head blight, the fungicide composition comprising validamycin and dicyanoanthraquinone in a mass ratio of 1-40:1-40.

[0019] Wherein, the mass ratio of the validamycin to the dicyanoanthraquinone includes but is not limited to any one of 1:1, 1:2, 1:3, 1:4, 1:5, 1:7, 1:9, 1:10, 1:13, 1:15, 1:17, 1:19, 1:20, 1:23, 1:25, 1:28, 1:30, 2:1, 3:1, 4:1, 5:1, 7:1, 9:1, 10:1, 13:1, 15:1, 17:1, 19:1, 20:1, 23:1, 25:1, 28:1, 30:1, 32:1, 34:1, 35:1, 38:1 or a range between any two of the above.

[0020] Among them, Jinggangmycin is an antibiotic produced by Streptomyces hygroscopicus, and it is a low-residue, environmentally safe pesticide, and it is safe to use in agricultural production. After Jinggangmycin comes into contact with the organism, it will be absorbed by the organism's cells and transported into the cell, and then hydrolyzed by β-D-glycosidase to produce Jinggang hydroxylamine. Jinggang hydroxylamine can inhibit the activity of trehalase, thereby affecting the energy metabolism of the organism and interfering with the normal growth of biological cells, thereby playing an antibacterial role. Jinggangmycin has an inhibitory effect on the growth of Fusarium graminearum, the wheat fusarium wilt fungus, affecting the normal growth of the pathogen, and with the increase of concentration, the mycelium branches more and more. Jinggangmycin can effectively inhibit the biosynthesis of DON toxin in Fusarium graminearum, reduce the production of DON toxin and the expression of key enzyme genes in DON toxin synthesis.

[0021] Dicyanoanthraquinone is an anthraquinone fungicide with good adhesion to the surface of crop plants. Dicyanoanthraquinone has multiple sites of action on pathogens: (1) The sulfhydryl groups on the dicyanoanthraquinone molecules interact with various enzymes in the fungus, interfering with the respiration of fungal cells and ultimately leading to the death of the pathogen; (2) affecting several sulfhydryl enzymes in the glycolysis pathway; and (3) affecting proteins, destroying the integrity of cell walls and cell membranes.

[0022] After a large number of experiments, the applicant found that using dicyanoanthraquinone, which has a different mechanism of action from commonly used control products, to control wheat ergot and compounding it with validamycin, which has a different mechanism of action from the agent, has a significant synergistic effect, can improve the control effect, slow down the occurrence of resistance, and reduce the amount of pesticide used and the cost of medication.

[0023] In order to further comprehensively consider the synergistic effect and the control effect, the present application optimizes the mass ratio of validamycin and dicyanoanthraquinone. Preferably, the bactericidal composition comprises validamycin and dicyanoanthraquinone in a mass ratio of 1-29:1-29.

[0024] More preferably, the bactericidal composition comprises validamycin and dicyanoanthraquinone in a mass ratio of 1-19:1-19.

[0025] More preferably, the bactericidal composition comprises validamycin and dicyanoanthraquinone in a mass ratio of 1-9:1-9.

[0026] More preferably, the bactericidal composition comprises validamycin and dicyanoanthraquinone in a mass ratio of 1-4:1-4.

[0027] In a second aspect, the present invention provides a bactericidal preparation, comprising the bactericidal composition as described above, and auxiliary materials.

[0028] This fungicide preparation adopts jinggangmycin and dicyanoanthraquinone. These two active ingredients have different mechanisms of action. The mixture of the two has obvious synergistic effect, which can greatly improve the efficacy and reduce the amount of pesticide used, while delaying the development of resistance to jinggangmycin and dicyanoanthraquinone.

[0029] It is understood that the bactericidal preparations include agriculturally acceptable solid preparations and liquid preparations. For example, solid preparations include but are not limited to wettable powders, water dispersible granules and granules, and liquid preparations include but are not limited to suspensions, microemulsions and aqueous emulsions.

[0030] Preferably, the mass of the bactericidal composition accounts for 10% to 80% of the total mass of the bactericidal preparation, including but not limited to any point value of 20%, 30%, 40%, 50%, 60%, 70% or a range value between any two of them.

[0031] It is to be understood that the auxiliary materials include any auxiliary materials acceptable in agriculture, such as adjuvants and carriers.

[0032] In some specific embodiments of the present invention, the auxiliary materials include at least one of solvents, emulsifiers, surfactants, wetting agents, dispersants, thickeners, antifreeze agents, film formers, capsule wall materials, pH acid-base regulators, disintegrants, preservatives, stabilizers, defoaming agents, colorants and fillers.

[0033] In a third aspect, the present invention provides a method for preparing the bactericidal preparation as described above, comprising the following steps:

[0034] Mix the bactericidal composition and auxiliary materials evenly.

[0035] The preparation method of the bactericidal preparation provided by the present invention has the advantages of being simple, easy to operate, having a short process flow, and being suitable for batch production.

[0036] In a fourth aspect, the present invention provides use of the fungicide composition or the fungicide formulation as described above in preventing and controlling fungal diseases of crops.

[0037] Preferably, the crop plant comprises wheat.

[0038] And / or, the fungal disease comprises fusarium head blight.

[0039] The bactericidal composition and bactericidal preparation provided by the present invention are used to prevent and treat fungal diseases of crops, especially for preventing and treating wheat fusarium head blight, which can not only improve the disease prevention effect, but also reduce the amount of pesticides used and slow down the generation of resistance.

[0040] Preferably, the field is sprayed at an application rate of 5 to 30 g (including but not limited to any one of 8 g, 10 g, 13 g, 15 g, 18 g, 20 g, 22 g, 25 g, 28 g or a range between any two) of the fungicide composition per mu.

[0041] More preferably, the field is sprayed at an application rate of 8 to 20 g of the fungicide composition per mu.

[0042] The embodiments of the present invention will be described in detail below in conjunction with the examples, but it will be appreciated by those skilled in the art that the following examples are only used to illustrate the present invention and should not be considered as limiting the scope of the present invention. If no specific conditions are specified in the examples, the conditions are carried out according to normal conditions or the conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be obtained commercially.

[0043] The purity of the Jinggangmycin technical used in the following examples and comparative examples of the present invention is 64%, and the manufacturer is Zhejiang Qianjiang Biochemical Co., Ltd.

[0044] The purity of the dicyanoanthraquinone technical used in the following examples and comparative examples of the present invention is 95%, and the manufacturer is Jiangxi Heyi Chemical Industry Co., Ltd.

[0045] The purity of the cyanothiocarb technical used in Comparative Example 4 of the present invention is 95%, and the manufacturer is Jiangsu Pesticide Research Institute Co., Ltd.

[0046] Example 1

[0047] The bactericidal composition provided in this embodiment is validamycin and dicyanoanthraquinone in a mass ratio of 29:1.

[0048] Example 2

[0049] The bactericidal composition provided in this embodiment is validamycin and dicyanoanthraquinone in a mass ratio of 19:1.

[0050] Example 3

[0051] The bactericidal composition provided in this embodiment is validamycin and dicyanoanthraquinone in a mass ratio of 9:1.

[0052] Example 4

[0053] The bactericidal composition provided in this embodiment is validamycin and dicyanoanthraquinone in a mass ratio of 4:1.

[0054] Example 5

[0055] The bactericidal composition provided in this embodiment is validamycin and dicyanoanthraquinone in a mass ratio of 1:1.

[0056] Example 6

[0057] The bactericidal composition provided in this embodiment is composed of validamycin and dicyanoanthraquinone in a mass ratio of 1:4.

[0058] Example 7

[0059] The bactericidal composition provided in this embodiment is validamycin and dicyanoanthraquinone in a mass ratio of 1:9.

[0060] Example 8

[0061] The bactericidal composition provided in this embodiment is validamycin and dicyanoanthraquinone in a mass ratio of 1:19.

[0062] Example 9

[0063] The bactericidal composition provided in this embodiment is validamycin and dicyanoanthraquinone in a mass ratio of 1:29.

[0064] Comparative Example 1

[0065] The bactericidal substance provided in this comparative example is Jinggangmycin.

[0066] Comparative Example 2

[0067] The fungicide provided in this comparative example is dicyanoanthraquinone.

[0068] Comparative Example 3

[0069] The fungicide provided in this comparative example is a carbendazim wettable powder with an active ingredient content of 50%.

[0070] Comparative Example 4

[0071] The fungicide provided in this comparative example is Jinggangmycin and cyanocbiconazole in a mass ratio of 29:1.

[0072] Experimental Example 1

[0073] Indoor toxicity test:

[0074] Experimental object: Wheat fusarium rust, obtained from Shandong Academy of Agricultural Sciences.

[0075] Drug preparation: Jinggangmycin is dissolved in sterile water, dicyanoanthraquinone is dissolved in acetone, and diluted with 0.1% Tween-80 solution to prepare single-dose stock solutions. A series of ratios are set, and each single dose and each group of mixed ratios are set with 5-6 gradient mass concentrations according to the geometric or arithmetic difference method.

[0076] Test method: Use solvent without active ingredients as control, and measure by hyphae growth rate method. Add the gradient series diluted drugs to the sterilized quantitative PDA medium in order, mix them thoroughly to make plates, and then make the expanded colonies into 0.5 cm cakes, put one piece in each dish, repeat 3 times for each treatment, and culture in a 28℃ constant temperature box. Measure the colony diameter for 4 days, calculate the hyphae growth inhibition rate, and calculate the toxicity regression equation to calculate EC 50 The results of the indoor toxicity test of the compositions of various embodiments against wheat fusarium are shown in Table 1.

[0077] The calculation formula of mycelium growth inhibition rate is as follows:

[0078] Mycelial growth inhibition rate = (blank control colony growth diameter - drug-treated colony growth diameter) / blank control colony growth diameter × 100;

[0079] When conducting the combined toxicity test of drugs, the Sun Yunpei method is used to calculate the co-toxicity coefficient of the mixture to evaluate the type of combined action of the mixture.

[0080] In this study, Jinggangmycin was used as the standard agent.

[0081] The relative toxicity index (TI) of a single dose of A A ) = EC of single dose A 50 Value / EC of single dose A 50 value × 100;

[0082] The relative toxicity index (TI) of a single dose of B B ) = EC of single dose A 50 Value / EC of single dose B 50 value × 100;

[0083] The measured toxicity index (ATI) of the mixture = (EC 50 / Test Mixture EC 50 )×100;

[0084] Theoretical toxicity index (TTI) of mixture = toxicity index of A agent (TI A )×mass percentage of A in the mixture + toxicity index of B (TI B )×the mass percentage of B in the mixture;

[0085] Co-toxicity coefficient (CTC) = [actual toxicity index (ATI) of the mixture / theoretical toxicity index (TTI) of the mixture] × 100.

[0086] According to Sun Yunpei's method: the synergistic effect of mixed pesticides is evaluated according to the co-toxicity coefficient method (CTC), that is, CTC < 80 is antagonistic, 80 < CTC < 120 is additive, and CTC > 120 is synergistic. The above formula is based on: "Pesticide Bioassay" edited by Shen Jinliang, NY-T 1156.2-2006 Pesticide Indoor Bioassay Test Guidelines Fungicides Part 2: Inhibition of Pathogenic Fungal Mycelial Growth Test Plate Method.

[0087] Table 1 Results of indoor toxicity test on wheat fusarium

[0088]

[0089]

[0090] As shown in Table 1, the EC values ​​of Jinggangmycin and dicyanoanthraquinone against wheat fusarium 50 The values ​​were 153.82 mg / L and 30.82 mg / L respectively. When the mass ratio of jinggangmycin to dicyanoanthraquinone was within a specific range, the co-toxicity coefficient was greater than 120, showing a synergistic effect. Especially when the mass ratio of jinggangmycin to dicyanoanthraquinone was 4:1, the co-toxicity coefficient was the largest at 193.32, with the best synergistic effect.

[0091] Experimental Example 2

[0092] Field trials for the prevention and control of wheat fusarium wilt:

[0093] The fungicidal compositions of Examples 1-9 and the fungicides of Comparative Examples 1, 2 and 4 were used to prepare fungicidal preparations, respectively. Comparative Example 3 was commercially available, and a field test for the prevention and control of wheat fusarium head blight was carried out.

[0094] The preparation methods of the bactericidal preparations in Examples 1-9 and Comparative Examples 1-2 are as follows: Jinggangmycin is dissolved in sterile water, dicyanoanthraquinone is dissolved in acetone, and diluted with a 0.1% Tween-80 solution to prepare a bactericidal preparation with a mass fraction of 20%.

[0095] The preparation method of the bactericidal preparation of Comparative Example 4 is as follows: Jinggangmycin is dissolved in sterile water, cyanocobalamin is dissolved in acetone, and then diluted with a Tween-80 solution with a mass fraction of 0.1% to prepare a bactericidal preparation with a mass fraction of 20%.

[0096] Spraying method: At the beginning of wheat flowering, the above-mentioned fungicides were diluted with water and sprayed. The second spray was conducted 7 days later. The water consumption was 20 kg per mu. Each treatment was repeated 4 times. Each plot was 50 m2. 2 The application rate is shown in Table 2 below. The occurrence of wheat scab was investigated at the milky stage, and the average control effect of the composition was calculated. The results are shown in Table 2 below.

[0097] Table 2 Field test results for wheat scab control

[0098]

[0099]

[0100] As can be seen from Table 2, the active ingredients of Comparative Example 1 only use Jinggangmycin, and the active ingredients of Comparative Example 2 only use dicyanoanthraquinone, and the control effect on wheat scab is poor. However, by compounding Jinggangmycin and dicyanoanthraquinone in each embodiment, the control effect on wheat scab can be greatly improved, and it is significantly higher than the control effect of each single agent treatment.

[0101] Furthermore, by comparing the embodiments and comparative example 3, it can be seen that the present invention can significantly reduce the amount of pesticide used by using validamycin and dicyanoanthraquinone in combination.

[0102] In addition, by comparing Example 1 and Comparative Example 4, it can be seen that the prevention effect of the combination of Jinggangmycin and dicyanoanthraquinone on wheat fusarium rust is significantly better than the prevention effect of the treatment in which the dicyanoanthraquinone is replaced by cyproconazole on wheat fusarium rust, and the dosage is lower.

[0103] In summary, the use of the bactericidal composition and bactericidal preparation provided by the present invention for preventing and controlling wheat fusarium head blight can not only improve the disease prevention effect, but also significantly reduce the amount of the agent used.

[0104] Although the present invention has been illustrated and described with specific embodiments, it should be appreciated that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Those skilled in the art should understand that the technical solutions described in the above embodiments may be modified, or some or all of the technical features thereof may be replaced by equivalents without departing from the spirit and scope of the present invention. However, these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention. Therefore, this means that all such replacements and modifications within the scope of the present invention are included in the appended claims.

Claims

1. A bactericidal composition, characterized in that The bactericidal composition comprises validamycin and dicyanoanthraquinone in a mass ratio of 1-40:1-40.

2. The bactericidal composition according to claim 1, characterized in that The bactericidal composition comprises validamycin and dicyanoanthraquinone in a mass ratio of 1-29:1-29.

3. The bactericidal composition according to claim 1, characterized in that The bactericidal composition comprises validamycin and dicyanoanthraquinone in a mass ratio of 1-19:1-19.

4. The bactericidal composition according to claim 1, characterized in that The bactericidal composition comprises validamycin and dicyanoanthraquinone in a mass ratio of 1-9:1-9.

5. A bactericidal preparation, characterized in that The invention comprises the bactericidal composition as claimed in any one of claims 1 to 4, and auxiliary materials.

6. The bactericidal preparation according to claim 5, characterized in that The mass of the bactericidal composition accounts for 10% to 80% of the total mass of the bactericidal preparation.

7. The method for preparing the bactericidal preparation according to claim 5 or 6, characterized in that: The steps include: Mix the bactericidal composition and auxiliary materials evenly.

8. Use of the fungicidal composition according to any one of claims 1 to 4 or the fungicidal preparation according to any one of claims 5 to 6 in preventing and controlling fungal diseases of crops.

9. The use according to claim 8, characterized in that The crops include wheat; And / or, the fungal disease comprises fusarium head blight.

10. The use according to claim 8, characterized in that The fungicide composition is sprayed on the field at an application rate of 5 to 30 g / mu.

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