Multi-phase VC mixture, storage and use method thereof, cosmetic and storage package

Abstract

The invention relates to a multiphase VC mixture, a storage and use method thereof, a cosmetic and a storage package. The multiphase VC mixture comprises 6-8 parts of VC, 8-10 parts of a water-soluble VC derivative and 4-6 parts of an oil-soluble VC derivative. The oil-soluble VC derivative is prepared from ascorbyl tetraisopalmitate, tetrahexyldecanol ascorbate and ascorbyl dipalmitate; the water-soluble VC derivative comprises VC ethyl ether, VC glucoside, VC magnesium phosphate and VC sodium phosphate. The prototype VC, the water-soluble VC derivative and the oil-soluble VC derivative are used in proportion to generate a synergistic effect, so that the effects of the VC, including removal of free radicals (DPPH) and inhibition of tyrosinase, are effectively improved, and in combination with a storage and use mode of an independent package of the prototype VC, fresh-keeping use of the VC is effectively realized, and the use effects of cosmetics are improved. By means of the storage package designed in different bins, different-bin sealed storage is achieved, and leakproofness of mixed use is achieved.

Description

Technical Field

[0001] The present invention relates to a multi-phase VC mixture, in particular to a multi-phase VC mixture and a storage and use method thereof, cosmetics and storage packaging, belonging to the technical field of cosmetics. Background Art

[0002] In the field of skin care, vitamin C (VC) has always attracted much attention. Prototype VC has many significant skin care effects. It can play a powerful antioxidant role, neutralize free radicals, and reduce the oxidative damage to the skin caused by external environmental factors such as ultraviolet rays and pollution. This antioxidant ability helps to delay skin aging. At the same time, prototype VC can also promote the production of collagen, increase the elasticity and firmness of the skin, and reduce the formation of wrinkles. For melanin deposition, prototype VC also has a proven whitening effect, which can inhibit tyrosinase activity and reduce the generated melanin, making the skin whiter and more lustrous.

[0003] However, prototype VC also has some shortcomings. First, its stability is poor and it is easily oxidized. This requires special attention during storage and use, such as keeping it away from light and sealed. Secondly, prototype VC is relatively irritating, especially high concentrations of prototype VC may cause skin allergies, redness and other discomfort symptoms; therefore, it cannot be used in large quantities and is not suitable for sensitive skin.

[0004] Oil-soluble VC derivatives have unique advantages in skin care. On the one hand, oil-soluble VC derivatives have better stability; on the other hand, due to their lipophilicity, oil-soluble VC derivatives are more easily absorbed by the skin. Common oil-soluble VC derivatives include ascorbyl tetraisopalmitate (VCIP), tetrahexyldecanol ascorbate (THD), etc. However, oil-soluble VC derivatives also have certain disadvantages. For oily skin, it may increase the greasiness of the skin and is not suitable for oily skin. Moreover, its scope of application is relatively narrow, and it may not be very friendly to some skin problems such as acne.

[0005] Water-soluble VC derivatives also have their own characteristics. The advantage is that they are more stable and refreshing than the original VC, and are suitable for all skin types, especially oily and mixed skin. Common water-soluble VC derivatives include VC ethyl ether, etc. However, water-soluble VC derivatives also have some shortcomings. Their antioxidant effect is weaker than that of the original VC, and their skin permeability is not as good as that of oil-soluble VC derivatives, making it difficult for them to penetrate deep into the skin to play a role.

[0006] There is currently no good solution on how to increase the usage of VC and its derivatives in cosmetics while ensuring their efficacy. Summary of the invention

[0007] In order to solve the deficiencies of the prior art, the object of the present invention is to provide a multi-phase VC mixture and a storage and use method thereof, a cosmetic and a storage package.

[0008] A multi-phase VC mixture, comprising, by weight, 4-10 parts of VC, 2-10 parts of a water-soluble VC derivative, and 1-8 parts of an oil-soluble VC derivative;

[0009] The oil-soluble VC derivatives include ascorbyl tetraisopalmitate (VC-IP), tetrahexyldecyl ascorbate (Tetrahexyldecyl Ascorbate), and ascorbyl dipalmitate (Ascorbyl Palmitate);

[0010] The water-soluble VC derivatives include VC ethyl ether, VC glucoside (Ascorbyl Glucoside), VC magnesium phosphate (Magnesium Ascorbyl Phosphate), and VC sodium phosphate (Sodium Ascorbyl Phosphate).

[0011] The above multi-phase VC mixture includes 6 parts of VC, 4 parts of water-soluble VC derivatives, and 10 parts of oil-soluble VC derivatives by mass.

[0012] The multi-phase VC mixture comprises, by mass, 6 parts of VC, 10 parts of ascorbic acid tetraisopalmitate, and 4 parts of VC ethyl ether.

[0013] The storage and use method of the multi-phase VC mixture comprises the following steps:

[0014] A1. When storing, the powdered VC is stored as the first unit, and the water-soluble VC derivative and the oil-soluble VC derivative are mixed and stored as the second unit;

[0015] A2. When using, mix the first unit into the second unit and mix well before use.

[0016] The above multi-phase VC mixture is used as a VC additive for cosmetics.

[0017] Furthermore, the addition amount of the above VC additive is 0.1-20wt%.

[0018] A cosmetic comprises the above-mentioned multi-phase VC mixture, wherein the VC is independently stored as a first unit.

[0019] A storage package, suitable for the above-mentioned multi-phase VC mixture and the above-mentioned cosmetics, comprises a first storage cavity and a second storage cavity; the first storage cavity is used to store VC, and the second storage cavity is used to store substances other than VC.

[0020] Furthermore, the above-mentioned storage package includes a bottle body and a bottle cap; the second storage cavity is the bottle cavity of the bottle body, and the first storage cavity is the cap cavity of the bottle cap closed by a pierceable structure.

[0021] The present invention is beneficial in that:

[0022] The multi-phase VC mixture and its storage and use method, cosmetics and storage packaging of the present invention achieve a synergistic effect by mixing powdered prototype VC, water-soluble VC derivatives and oil-soluble VC derivatives in a specific proportion, thereby effectively improving the efficacy of VC, including the scavenging of free radicals (DPPH) and the inhibition of tyrosinase. Combined with the storage and use of the prototype VC independent packaging, the freshness of VC is effectively locked and the efficacy of the cosmetics is improved.

[0023] The storage package of the present invention is designed with compartments, which not only realizes sealed storage in compartments, but also realizes airtightness for mixed use, retains the maximum original characteristics of the raw materials, is simple to use and does not require contact with the material body through other objects during use, thus avoiding the risk of the material body being contaminated before use, and is easy to carry and use, and has strong practicality and wide applicability. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 Diagram of the initial states of the cosmetics prepared in Examples 1 and 3 (A, Example 1, B, Example 3).

[0025] Figure 2 The diagrams are of the states of the cosmetics of Examples 1 and 2 after being placed in a constant temperature oven (C, Example 1, D, Example 2).

[0026] Figure 3 The state diagram of the components other than VC of Example 3 and Example 4 after being placed in a constant temperature oven (E, Example 3, F, Example 4).

[0027] Figure 4 The state diagram of VC of Example 3 and Example 4 after being placed in a constant temperature oven for 20 days (G, Example 3, H, Example 4).

[0028] Figure 5 The diagrams are of the states of the cosmetics of Examples 5 and 6 (J, Example 5, K, Example 6).

[0029] Figure 6 This is a comparative test chart for free radical (DPPH) scavenging.

[0030] Figure 7 This is a comparative experimental diagram for inhibiting tyrosinase.

[0031] Figure 8 A schematic diagram of the storage package structure.

[0032] The meanings of the marks in the accompanying drawings are as follows: 1. bottle body, 2. cover body, 3. material pocket, 4. key cap, 5. limiting ring, 6. material storage cavity. DETAILED DESCRIPTION

[0033] The present invention is described in detail below with reference to the accompanying drawings and specific embodiments.

[0034] The multi-phase VC mixture consists of 4-10 parts of VC, 2-10 parts of water-soluble VC derivatives, and 1-10 parts of oil-soluble VC derivatives in parts by mass.

[0035] The oil-soluble VC derivative can be selected from one or more of ascorbyl tetraisopalmitate (VC-IP), tetrahexyldecyl ascorbate, and ascorbyl dipalmitate (Ascorbyl Palmitate).

[0036] The water-soluble VC derivative can be selected from one or more of VC ethyl ether, VC glucoside (Ascorbyl Glucoside), VC magnesium phosphate (Magnesium Ascorbyl Phosphate), and VC sodium phosphate (Sodium Ascorbyl Phosphate).

[0037] The formula components of the multi-phase VC mixture are shown in Table 1 below:

[0038]

[0039]

[0040] Table 1

[0041] The storage and use method of the multi-phase VC mixture comprises the following steps:

[0042] A1. When storing, the powdered VC is stored as the first unit, and the water-soluble VC derivative and the oil-soluble VC derivative are mixed and stored as the second unit;

[0043] A2. When using, mix the first unit into the second unit and mix well before use.

[0044] The multiphase VC mixture is used as a VC additive for cosmetics, and the addition amount is preferably 0.1-20wt%.

[0045] A cosmetic comprises a multi-phase VC mixture and other materials, wherein the VC is independently stored as a first unit.

[0046] Recipe 6:

[0047]

[0048] Table 2

[0049] Recipe 7:

[0050]

[0051]

[0052] Table 3

[0053] A. Stability test:

[0054] The cosmetics composed of the components of the multi-phase VC mixture of the above formula 1 and the remaining materials of the formula 6 in the above Table 2 were used as experimental objects for stability test.

[0055] A1. Comparative test 1

[0056] According to the components in Table 2 above, all raw materials No. 1-16 were mixed completely at room temperature and poured into a glass bottle. Two portions were prepared and placed in a constant temperature oven at room temperature (Example 1) and 48° C. (Example 2) for 20 days to investigate the stability.

[0057] A2. Test 1

[0058] According to the components in Table 2 above, the raw materials No. 1-15 were first mixed at room temperature and poured into the same glass bottle, and then the VC raw material No. 16 (powder) was poured into the powder bin above the same glass bottle. The mixture was divided into 2 parts and placed in a constant temperature oven at room temperature (Example 3) and 48° C. (Example 4) for 20 days to investigate the stability.

[0059] A3. Test 2

[0060] Example 5: The VC in Example 3 that had been placed in a constant temperature oven was mixed into the liquid in the glass bottle, so that the VC was dissolved in the aqueous phase layer, and the stability was observed.

[0061] Example 6: The VC in Example 4 that had been placed in a constant temperature oven was mixed into the liquid in the glass bottle, so that the VC was dissolved in the aqueous phase layer, and the stability was observed.

[0062] B. Stability test results

[0063] B1. Figure 1 , which is a diagram of the initial state of the cosmetics of comparative test 1 (Example 1 or 2) and test (Example 3 or 4) after preparation.

[0064] As shown in the figure, there are oil and water stratifications, the upper part is the red transparent oil phase layer, and the lower part is the colorless transparent water phase layer.

[0065] B2, such as Figure 2 The diagram shows the state of Example 1 and Example 2 after being placed in a constant temperature oven for 20 days. It can be seen that the water phase layer has obviously turned yellow, and the stability of the water phase layer has changed.

[0066] B3, such as Figure 3 The figure shows the state of Example 3 and Example 4 after being placed in a constant temperature oven for 20 days. It can be seen that there is no color change in the oil phase layer and the water phase layer. That is, in the case of no VC mixed in, the liquid containing the water-soluble VC derivative and the oil-soluble VC derivative in the bottle has good stability.

[0067] B4, such as Figure 4 The figure shows the state of VC of Example 3 and Example 4 after being placed in a constant temperature oven for 20 days. It can be seen that the color of VC has not changed, indicating that the stability of VC stored in the powder bin is good.

[0068] B5. Figure 5 The diagrams are state diagrams of Example 5 and Example 6. It can be seen that after being placed in a constant temperature oven at room temperature and 48 degrees for 20 days, the VC in the powder bin and the liquid in the glass bottle are mixed and the VC is dissolved in the water phase layer, and the water phase layer is still colorless and transparent. That is, the cosmetics have good stability.

[0069] The above stability test results show that the mixing and storage method of the components in Experiment 1 cannot maintain the stability of the components, especially VC, resulting in rapid oxidation and yellowing of the material, thereby weakening the efficacy and activity of VC. The mixing and storage method of the components in Experiment 1 is obviously more conducive to the stable storage of each component, especially VC.

[0070] C. Synergistic effect of VC, oil-soluble VC derivatives, and water-soluble VC derivatives

[0071] The cosmetics composed of the components of the multiphase VC mixture of the above formula 1, formula 4, and formula 5 and the remaining materials in the following Table 4 were used as experimental objects for efficacy tests.

[0072]

[0073] Table 4

[0074] C1. Comparative test of free radical (DPPH) scavenging

[0075] C11. Test methods

[0076]

[0077] Table 5C12. Test materials

[0078] Reagents / consumables Manufacturer Specification 1,1-Diphenyl-2-trinitrophenylhydrazine Source Leaf 98%-250mg 95% ethanol Shanghai Boer Chemical Reagent Co., Ltd. 500ml Vitamin E Adamas 99%-100mg

[0079] Table 6

[0080] C13. Test solution preparation

[0081] Sample preparation:

[0082] (1) Prepare three test samples 1#, 2#, and 3# according to the formula in Table 4.

[0083] (2) Dilute the 1#, 2#, and 3# test samples with 95% ethanol to Multi-level concentration samples , weigh 0.1g of the sample and dissolve it in 100mL of high-purity water. After stirring, mixing, precipitation and centrifugation, take 2.50mL, 5.00mL and 10.00mL of the upper clear solution respectively, make up to 50mL with high-purity water, mix well and test (the sample concentrations are 0.05, 0.10 and 0.20mg / mL respectively).

[0084] Reagent preparation:

[0085] (1) 0.12 mg / mL DPPH ethanol solution:

[0086] Weigh 12 mg of 1,1-diphenyl-2-trinitrophenylhydrazine into a 250 mL beaker, add 100 mL of 95% ethanol, and stir with a glass rod until dissolved.

[0087] (2) Weigh 0.04 g of the positive control substance (vitamin E) and add 95% ethanol to make up to 100 ml. Pipette 2 ml, 1 ml, 0.5 ml, and 0.25 ml into a 10 ml volumetric flask, respectively, and dilute with 95% ethanol to make a series of concentration gradients: 0.08 mg / mL, 0.04 mg / mL, 0.02 mg / mL, and 0.01 mg / mL to verify the test system.

[0088] C14. Test steps

[0089] See Table 7 below. Use 10 mL test tubes to set up sample tubes, sample background tubes, DPPH tubes, and solvent background tubes.

[0090] For each sample, five parallel tubes should be set up for each tested concentration, and three parallel tubes should be set up for the DPPH tube.

[0091] Sample tube Sample background tube DPPH tube Solvent background tube Sample solution (mL) 2 2 —— —— Water or 95% ethanol solvent (mL) 4 4 6 6 DPPH ethanol solution (mL) 2 —— 2 —— 95% ethanol (mL) —— 2 —— 2 Parallel times 5 / sample 1 / sample 3 / Experimental 1 / Experiment

[0092] Table 7C15, calculation formula:

[0093]

[0094] In the formula, T is the absorbance of the sample tube, that is, the absorbance of the solution after the sample reacts with DPPH; T0 is the sample background absorbance; C is the three average values ​​of the DPPH tube absorbance, that is, the absorbance of the DPPH solution when no sample is added; Co is the solvent background tube absorbance.

[0095] C16. Results and Conclusions

[0096] The test results are shown in Table 8 and Figure 6 :

[0097]

[0098] Table 8

[0099] After comparison, among the three samples (solution concentrations were 0.05 mg / ml, 0.1 mg / ml, and 0.2 mg / ml respectively), sample 1# had the highest DPPH clearance rate.

[0100] C2. Comparative experiment on inhibition of tyrosinase

[0101] C21. Method based on standard

[0102]

[0103] Table 9C22. Experimental Materials

[0104] Reagents / consumables Manufacturer Specification Disodium hydrogen phosphate dodecahydrate Aladdin ≥99%, AR500g Sodium dihydrogen phosphate dihydrate Greagent ≥99%, AR500g L-Tyrosine Adamas 99%RG25g Kojic Acid Maclean 99％25g Tyrosinase Maclean 25ku 95% ethanol Shanghai Boer Chemical Reagent Co., Ltd. 500ml

[0105] Table 10

[0106] C23. Test solution preparation

[0107] Sample preparation:

[0108] (1) Prepare three test samples 1#, 2#, and 3# according to the formula in Table 4.

[0109] (2) Weigh 0.1 g of each sample and dissolve it in 20 mL of high-purity water. After stirring and mixing, take 10.00 mL of the solution, dilute to 50 mL with high-purity water, and mix well for testing (sample concentration is 1.00 mg / mL).

[0110] Reagent preparation:

[0111] (1) Disodium hydrogen phosphate solution (0.2 mol / L)

[0112] Weigh 35.8158 g of disodium hydrogen phosphate dodecahydrate (Na2HPO4·12H2O), dissolve it in 500 mL of high-purity water, and mix well.

[0113] (2) Sodium dihydrogen phosphate solution (0.2 mol / L)

[0114] Weigh 15.6018 g of sodium dihydrogen phosphate dihydrate (NaH2PO4·2H2O), dissolve it in 500 mL of high-purity water, and mix well.

[0115] (3) Phosphate buffered saline (PBS, pH = 6.8)

[0116] Measure 408 mL of sodium dihydrogen phosphate solution (0.2 mol / L) and 392 mL of sodium dihydrogen phosphate solution (0.2 mol / L), and mix them well.

[0117] (4) L-tyrosine solution (0.15mmol / L)

[0118] Weigh 0.0272 g of L-tyrosine, pre-disperse it with phosphate buffer (PBS, pH=6.8), sonicate for 30 min, make up to volume with phosphate buffer (PBS, 0.2 mol / L, pH=6.8) in a 100 mL volumetric flask, and mix well.

[0119] (5) Tyrosinase solution (0.05 mg / mL)

[0120] Weigh 0.0045 g of tyrosinase powder, dissolve it in phosphate buffer (PBS, 0.2 mol / L, pH=6.8), make up to 90 mL, and mix well.

[0121] C24. Test steps

[0122] (1) See Table 11 below. Use 10 mL test tubes to set up sample tubes, sample background tubes, tyrosinase tubes, and solvent background tubes.

[0123] (2) For each sample, five parallel tubes should be set up for each tested concentration, and three parallel tubes should be set up for the tyrosinase tube.

[0124]

[0125]

[0126] Table 11C25, calculation formula

[0127]

[0128] C26. Results and Conclusions

[0129] The experimental results are shown in Table 12 and Figure 7 :

[0130]

[0131] Table 12

[0132] After comparison, among the three samples (the solution concentrations were 0.25 mg / ml, 0.5 mg / ml, and 1.0 mg / ml respectively), sample 1# had the highest tyrosinase inhibition rate.

[0133] A storage package, suitable for a multi-phase VC mixture and the above-mentioned cosmetics, preferably comprises a bottle body and a bottle cap, wherein the bottle cavity of the bottle body is the second storage cavity, and the cover cavity of the bottle cap is the first storage cavity.

[0134] The VC (powder) in the cosmetics is stored in the first storage chamber, which is sealed by a pierceable structure. The remaining raw materials in the cosmetics except the VC are stored in the bottle body.

[0135] like Figure 8 The figure shows a schematic diagram of the structure of the storage package.

[0136] The storage package is composed of a bottle body and a bottle cap, wherein the bottle cap is composed of a cover body 2, a key cap 4 and a material pocket 3.

[0137] The cover body 2 is provided with an internal thread matching the bottle mouth of the bottle body 1, and the top of the cover body 2 is open. A limiting ring 5 is provided on the inner side wall of the cover body 2 at the top of the internal thread.

[0138] The cross section of the material pocket 3 is U-shaped, and a transverse annular groove is provided on the outer wall of the pocket opening; the inner ring of the limiting ring 5 is fixed by the groove, and the material pocket 3 is suspended in the cover body 2.

[0139] The cross section of the keycap 4 is T-shaped, consisting of a horizontal part and a vertical part. The vertical part is hollow and open at the bottom to form a material storage chamber 6; preferably, the cross section of the bottom end of the vertical part is an inclined surface. The keycap 4 is placed in the cover body 2 from the top opening of the cover body 2, its vertical part is placed in the material pocket 3, and the horizontal part is embedded in the cover body 2 at the top of the limiting ring 5. When the bottom end of the vertical part of the keycap 4 abuts against the bottom of the material pocket 3, there is a certain vertical gap between the horizontal part of the keycap 4 and the limiting ring 5, and the top surface of the keycap 4 is higher than the top opening of the cover body 2.

[0140] When storing cosmetics,

[0141] The bottle body 1 is filled with the remaining raw materials except VC.

[0142] The key cap 4 is turned upside down, and the powdered VC is filled into the material storage cavity 6. Then the cover body 2 of the suspended material pocket 3 is turned upside down on the key cap 4, and the material storage cavity 6 is blocked by the bottom of the material pocket 3.

[0143] Then turn the entire bottle cap upside down and tighten the bottle cap to the bottle body to complete the storage of the cosmetics.

[0144] When using cosmetics,

[0145] Press the key cap 4 to make it slide down along the inner wall of the cover body 2 until the bottom end of the vertical part of the key cap 4 pierces the bottom of the material pocket 3, so that the stored VC falls into the bottle body 1 and is mixed with the other raw materials to form the cosmetic of Example 5 for use.

[0146] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the above embodiments do not limit the present invention in any form, and any technical solution obtained by equivalent replacement or equivalent transformation falls within the protection scope of the present invention.

Claims

1. A multiphase VC mixture, characterized in that: In parts by mass, it comprises 4-10 parts of VC, 2-10 parts of water-soluble VC derivatives, and 1-10 parts of oil-soluble VC derivatives; The oil-soluble VC derivatives include ascorbyl tetraisopalmitate, tetrahexyldecyl ascorbate, and ascorbyl dipalmitate; The water-soluble VC derivatives include VC ethyl ether, VC glucoside, VC magnesium phosphate, and VC sodium phosphate.

2. The multiphase VC mixture according to claim 1, characterized in that: By mass, it includes 6 parts of VC, 4 parts of water-soluble VC derivatives, and 10 parts of oil-soluble VC derivatives.

3. The multiphase VC mixture according to claim 2, characterized in that: In parts by mass, it includes 6 parts of VC, 10 parts of ascorbic acid tetraisopalmitate, and 4 parts of VC ethyl ether.

4. The method for storing and using a multi-phase VC mixture according to claim 1, characterized in that: The following steps are involved: A1. When storing, the powdered VC is stored as the first unit, and the water-soluble VC derivative and the oil-soluble VC derivative are mixed and stored as the second unit; A2. When using, mix the first unit into the second unit and mix well before use.

5. The multi-phase VC mixture according to claim 1, characterized in that: Application as VC additive for cosmetics.

6. The multi-phase VC mixture according to claim 5, characterized in that: The added amount of the VC additive is 0.1-22wt%.

7. A cosmetic, characterized in that: It comprises the multi-phase VC mixture as claimed in claim 1, and the VC is independently stored as a first unit.

8. A storage package, characterized in that: Applicable to the multi-phase VC mixture of claim 1 and the cosmetic of claim 6, comprising a first storage chamber and a second storage chamber; The first storage chamber is used to store VC, and the second storage chamber is used to store substances other than VC.

9. A storage package according to claim 8, characterized in that: It comprises a bottle body and a bottle cap; the second storage cavity is the bottle cavity of the bottle body, and the first storage cavity is the cap cavity of the bottle cap closed by a puncturable structure.

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