Preparation process of eye-moistening spray and spray

Abstract

The present invention discloses a preparation process and a spray of an eye-moistening spray, and belongs to the technical field of eye-moistening liquid. The eye-moistening spray comprises a spray bottle and an eye-moistening liquid, and the eye-moistening liquid comprises the following raw materials: sea salt, sodium hyaluronate, lutein, cassia seed extract, wild chrysanthemum extract, borneol, Hainan pea vine extract, and the balance is water. The present invention innovatively combines Hainan pea vine with lutein, and through compounding the two, the efficacy of lutein in relieving eye turbidity is significantly enhanced. At the same time, an advanced low-temperature ultra-high pressure synergistic pretreatment technology is adopted for wild chrysanthemum, and efficient extraction is carried out on this basis. This process effectively reduces its surface tension, thereby greatly improving the eye-moistening comfort of the product. Compounding Hainan pea vine with wild chrysanthemum that has been specially pretreated not only greatly promotes the dual improvement of the eye-moistening effect and the ability of lutein to improve eye turbidity, but also unexpectedly shows a significant effect on alleviating the phenomenon of red bloodshot eyes.

Description

Technical Field

[0001] The invention relates to the technical field of eye-lubricating liquids, and in particular to a preparation process of an eye-lubricating spray and the spray. Background Art

[0002] With the high frequency use of electronic products such as computers and mobile phones, more and more people are suffering from conjunctivitis, keratitis or visual fatigue, which in turn causes dry eyes, turbidity or red bloodshot eyes. Although there are many types of eye drops that can relieve visual fatigue, some patients are worried that long-term use of the drug will lead to drug dependence. Based on this, the development of eye wash or eye spray that can effectively clean the eyes, improve the eye environment and relieve visual fatigue has broad market prospects.

[0003] For example, Chinese patent CN115487143B discloses a sodium hyaluronate eye drop and a preparation method thereof, which belongs to the field of eye drops. Each 400ml eye drop includes the following components by weight: 0.3-0.5g of modified sodium hyaluronate, 3-5g of sodium chloride, 0-2g of potassium chloride, 0.2-0.4g of boric acid, 0.01-0.03g of borax, and the remainder is water for injection. The modified sodium hyaluronate is prepared by the following method: dissolving sodium hyaluronate in water, adding a catalyst thereto, and adjusting the pH value of the solution to 5.1-5.5, then adding a thiol reagent to the solution, adjusting the pH value of the solution to 4.8-5.0, stirring the reaction, then dialyzing to remove the catalyst and impurities, and drying to obtain. It can effectively solve the problems of short lubrication time and high frequency of dripping in existing eye drops.

[0004] However, although the above patent can prolong the lubrication time and reduce the frequency of dripping, it cannot improve the problem of eye turbidity or red bloodshot.

[0005] Based on this, the present invention designs a preparation process and a spray for eye moisturizing spray to solve the above problems. Summary of the invention

[0006] In view of the above-mentioned shortcomings of the prior art, the present invention provides a preparation process and a spray for eye moisturizing spray.

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0008] An eye-moistening spray comprises a spray bottle and an eye-moistening liquid. The eye-moistening liquid comprises the following raw materials: 0.8-1.1% sea salt, 0.1-0.15% sodium hyaluronate, 0.01-0.04% lutein, 0.02-0.4% cassia seed extract, 0.05-0.7% wild chrysanthemum extract, 0.005-0.009% borneol, 0.008-0.02% hainan pea vine extract, and the balance is water.

[0009] A preparation process of the eye spray comprises the following steps:

[0010] Step 1: preparing wild chrysanthemum extract;

[0011] 1) Crushing: crush the wild chrysanthemum into 20-40 meshes by a crusher;

[0012] 2) Low-temperature and ultra-high pressure synergistic pretreatment: The crushed wild chrysanthemum is placed in an immersion freezing device with -40~-30℃ liquid alcohol as the freezing medium. When the center temperature of the crushed wild chrysanthemum reaches -5~-2℃, the freezing is completed and ultra-high pressure treatment is performed;

[0013] 3) Extraction: put the pretreated wild chrysanthemum into the extraction tank, then add 60% ethanol solution with a weight of 9-12 times the weight of the raw material and a temperature of 40-45°C, add cellulase, pectinase and decarboxylase respectively, and release the enzymatic extract after enzymolysis for 50-60 minutes;

[0014] 4) Filtration and centrifugation;

[0015] 5) Evaporation and concentration;

[0016] 6) Sterilization;

[0017] 7) Low temperature drying to obtain wild chrysanthemum extract;

[0018] Step 2: preparing the extract of Millettia hainanensis by subcritical extraction;

[0019] 1) Grind the Hainan Millettia vine into powder and completely immerse it in a sodium carbonate solution at 38-46°C for 1-2 hours, filter and dry the powder, and repeat the alkali washing 2-3 times;

[0020] 2) adding the alkali-washed Millettia hainanensis powder into a subcritical extraction kettle, using water as an extractant, with a mass ratio of water to Millettia hainanensis powder of 8-9:1, to obtain an extract;

[0021] 3) filtering to remove solid impurities to obtain an extract, and evaporating and concentrating the extract to obtain a Millettia hainanensis extract;

[0022] Step 3: Add lutein to the solution containing the Hainan Millettia oleifera extract, stir at 40-50°C; cool to room temperature, add sodium hyaluronate, borneol and wild chrysanthemum extract mixed with cassia seed extract in sequence, and stir for 30-40 minutes;

[0023] Step 4: Add sea salt solution to dilute to 0.01-0.04% lutein to obtain eye drops;

[0024] Step 5: Pour the eye drops into the spray bottle to get the eye spray.

[0025] Furthermore, step 2) is specifically as follows: placing the crushed wild chrysanthemum in an immersion freezing device with -40~-30°C liquid alcohol as a freezing medium, and the freezing is completed when the center temperature of the crushed wild chrysanthemum reaches -5~-2°C, and performing ultra-high pressure treatment at 200~250Mpa for 1~5min.

[0026] Furthermore, step 3) is specifically as follows: the pretreated wild chrysanthemum is placed in an extraction tank, and then a 60% ethanol solution with a weight of 9 to 12 times the weight of the raw material and a temperature of 40 to 45°C is added, and 0.2 to 0.3% of cellulase, 0.2 to 0.3% of pectinase and 0.05 to 0.1% of decarboxylase based on the weight of the wild chrysanthemum are added respectively, and after enzymolysis for 50 to 60 minutes, the enzymolysis extract is released.

[0027] Furthermore, in step 1, the centrifugal speed of step 4) is 6000~8000r / min.

[0028] Furthermore, in step 1, in step 6), the sterilization temperature is 135-137° C. and the time is 25-30 seconds.

[0029] Furthermore, in step 1, the drying temperature in step 7) is 40-60°C.

[0030] Furthermore, in step 2, the Hainan Millettia vine is ground into a powder of 10-40 mesh and then completely immersed in a sodium carbonate solution with a pH of 9-10 at 38-46°C for 1-2 hours of alkali washing, and the powder is filtered and dried and then the alkali washing is repeated 2-3 times.

[0031] Furthermore, in step 2, the pressure of subcritical extraction is 0.6-0.7 MPa, the extraction temperature is 150-180° C., and the extraction time is 20-25 min.

[0032] Furthermore, step three is specifically as follows: adding lutein to a solution containing 20-30% of Millettia hainanensis extract, stirring at 40-50°C and 180-200r / min for 20-24min; cooling to room temperature, adding sodium hyaluronate, borneol and wild chrysanthemum extract mixed with cassia seed extract in sequence, and stirring at 180-200r / min for 30-40min.

[0033] Compared with the prior art, the present invention has the following beneficial effects: the present invention innovatively combines Millettia hainanensis and lutein, and through the compounding of the two, the efficacy of lutein in relieving eye turbidity is significantly enhanced. At the same time, advanced low-temperature and ultra-high pressure synergistic pretreatment technology is adopted for wild chrysanthemum, and efficient extraction is carried out on this basis. This process effectively reduces its surface tension, thereby greatly improving the eye-soothing comfort of the product. It is particularly worth mentioning that the compounding and application of Millettia hainanensis and wild chrysanthemum that has undergone special pretreatment not only greatly promotes the dual improvement of the eye-soothing effect and the ability of lutein to improve eye turbidity, but also unexpectedly shows a significant effect on reducing the phenomenon of red bloodshot eyes, achieving comprehensive optimization of eye health maintenance. DETAILED DESCRIPTION

[0034] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] Embodiment 1: This embodiment provides a preparation process of an eye spray, comprising the following steps:

[0036] Step 1: preparing wild chrysanthemum extract;

[0037] 1) Crushing: crush the wild chrysanthemum into 40 meshes by a crusher;

[0038] 2) Low-temperature and ultra-high pressure synergistic pretreatment: The crushed wild chrysanthemum is placed in an immersion freezing device with -30℃ liquid alcohol as the freezing medium. The freezing is completed when the center temperature of the crushed wild chrysanthemum reaches -2℃, and ultra-high pressure treatment is performed at 250Mpa for 5 minutes.

[0039] 3) Extraction: put the pretreated wild chrysanthemum into an extraction tank, then add a 60% ethanol solution with a weight of 12 times the weight of the raw material and a temperature of 45°C, add 0.3% cellulase based on the weight of the wild chrysanthemum, 0.3% pectinase based on the weight of the raw material and 0.1% decarboxylase based on the weight of the raw material, respectively, and release the enzymatic extract after 60 minutes of enzymolysis;

[0040] 4) Filtration centrifugation: speed 8000r / min;

[0041] 5) Evaporation and concentration;

[0042] 6) Sterilization: Ultra-high temperature instantaneous sterilization is used, the sterilization temperature is 137°C, and the time is 30s;

[0043] 7) Low temperature drying: Drying at 60°C to obtain the wild chrysanthemum extract;

[0044] Step 2: preparing the extract of Millettia hainanensis by subcritical extraction;

[0045] 1) Grind the hainan pea vine into a 40 mesh powder and completely immerse it in a 46°C sodium carbonate solution with a pH of 10 for 2 hours, filter and dry the powder, and repeat the alkali washing three times;

[0046] 2) adding the alkali-washed Millettia hainanensis powder into a subcritical extraction kettle, using water as the extractant, the mass ratio of water to Millettia hainanensis powder being 9:1; the pressure being 0.7 MPa, the extraction temperature being 180°C, the extraction time being 25 min, and obtaining an extract;

[0047] 3) filtering to remove solid impurities to obtain an extract, and evaporating and concentrating the extract to obtain a Millettia hainanensis extract;

[0048] Step 3: weigh the raw materials: 1.1 kg sea salt, 0.15 kg sodium hyaluronate, 0.04 kg lutein, 0.4 kg cassia seed extract, 0.7 kg wild chrysanthemum extract, 0.009 kg borneol, 0.02 kg hainan pea extract;

[0049] Step 4: Add lutein to a solution containing 30% of the Hainan Millettia oleifera extract, stir at 50°C and 200 r / min for 24 min; cool to room temperature, add sodium hyaluronate, borneol and wild chrysanthemum extract mixed with cassia seed extract in sequence, and stir at 200 r / min for 40 min;

[0050] Step 5: Add sea salt solution to dilute to 0.04% lutein to obtain eye drops;

[0051] Step 6: Pour the eye drops into the spray bottle to get the eye spray.

[0052] After testing, the pH value of the eye spray prepared in this embodiment is in the range of 7.3-7.6, the osmotic pressure is in the range of 280-310mOsm / kgH2O, and the total bacterial colony count is ≤90cfu / mL, which meets the industry standards.

[0053] Embodiment 2: This embodiment provides a preparation process of an eye spray, comprising the following steps:

[0054] Step 1: preparing wild chrysanthemum extract;

[0055] 1) Crushing: crush the wild chrysanthemum into 20 meshes by a crusher;

[0056] 2) Low-temperature and ultra-high pressure synergistic pretreatment: The crushed wild chrysanthemum is placed in an immersion freezing device with -40℃ liquid alcohol as the freezing medium. The freezing is completed when the center temperature of the crushed wild chrysanthemum reaches -5℃, and ultra-high pressure treatment is performed at 200Mpa for 1 minute.

[0057] 3) Extraction: put the pretreated wild chrysanthemum into an extraction tank, then add a 60% ethanol solution with a weight of 9 times the weight of the raw material and a temperature of 40°C, add 0.2% cellulase based on the weight of the wild chrysanthemum, 0.2% pectinase based on the weight of the raw material and 0.05% decarboxylase based on the weight of the raw material, respectively, and release the enzymatic extract after enzymolysis for 50 minutes;

[0058] 4) Filtration centrifugation: speed 6000r / min;

[0059] 5) Evaporation and concentration;

[0060] 6) Sterilization: Ultra-high temperature instantaneous sterilization is used, the sterilization temperature is 135°C, and the time is 25s;

[0061] 7) Low temperature drying: Drying at 40°C to obtain the wild chrysanthemum extract;

[0062] Step 2: preparing the extract of Millettia hainanensis by subcritical extraction;

[0063] 1) Grind the hainan pea vine into a 10-mesh powder and completely immerse it in a sodium carbonate solution with a pH of 9 at 38°C for 1 hour, filter and dry the powder, and repeat the alkali washing twice;

[0064] 2) adding the alkali-washed Millettia hainanensis powder into a subcritical extraction kettle, using water as the extractant, the mass ratio of water to Millettia hainanensis powder being 8:1; the pressure being 0.6 MPa, the extraction temperature being 150°C, the extraction time being 20 min, and obtaining an extract;

[0065] 3) filtering to remove solid impurities to obtain an extract, and evaporating and concentrating the extract to obtain a Millettia hainanensis extract;

[0066] Step 3: weigh the raw materials: 0.8kg sea salt, 0.1kg sodium hyaluronate, 0.01kg lutein, 0.02kg cassia seed extract, 0.05kg wild chrysanthemum extract, 0.005kg borneol, 0.008kg hainan pea extract;

[0067] Step 4: Add lutein to a solution containing 20% ​​of the extract of Millettia hainanensis, stir at 40°C and 180r / min for 20 minutes; cool to room temperature, add sodium hyaluronate, borneol and wild chrysanthemum extract mixed with cassia seed extract in sequence, and stir at 180r / min for 30 minutes;

[0068] Step 5: Add sea salt solution to dilute to 0.01% lutein to obtain eye drops;

[0069] Step 6: Pour the eye drops into the spray bottle to get the eye spray.

[0070] Embodiment 3: This embodiment provides a preparation process of an eye spray, comprising the following steps:

[0071] Step 1: preparing wild chrysanthemum extract;

[0072] 1) Crushing: crush the wild chrysanthemum into 40 meshes by a crusher;

[0073] 2) Low-temperature and ultra-high pressure synergistic pretreatment: The crushed wild chrysanthemum is placed in an immersion freezing device with -38℃ liquid alcohol as the freezing medium. When the center temperature of the crushed wild chrysanthemum reaches -4℃, the freezing is completed, and ultra-high pressure treatment is performed at 220Mpa for 2 minutes;

[0074] 3) Extraction: put the pretreated wild chrysanthemum into an extraction tank, then add a 60% ethanol solution with a weight of 10 times the weight of the raw material and a temperature of 41°C, add 0.3% cellulase based on the weight of the wild chrysanthemum, 0.2% pectinase based on the weight of the raw material, and 0.05% decarboxylase based on the weight of the raw material, respectively, and release the enzymatic extract after enzymolysis for 52 minutes;

[0075] 4) Filtration centrifugation: speed 6000r / min;

[0076] 5) Evaporation and concentration;

[0077] 6) Sterilization: Ultra-high temperature instantaneous sterilization is used, the sterilization temperature is 135°C, and the time is 28s;

[0078] 7) Low temperature drying: Drying at 55°C to obtain the wild chrysanthemum extract;

[0079] Step 2: preparing the extract of Millettia hainanensis by subcritical extraction;

[0080] 1) Grind the hainan pea vine into a 20-mesh powder and completely immerse it in a 41°C sodium carbonate solution with a pH of 9 for 2 hours. Filter and dry the powder and repeat the alkali washing for 3 times.

[0081] 2) adding the alkali-washed Millettia hainanensis powder into a subcritical extraction kettle, using water as the extractant, the mass ratio of water to Millettia hainanensis powder being 9:1; the pressure being 0.7 MPa, the extraction temperature being 170°C, the extraction time being 24 min, and obtaining an extract;

[0082] 3) filtering to remove solid impurities to obtain an extract, and evaporating and concentrating the extract to obtain a Millettia hainanensis extract;

[0083] Step 3: weigh the raw materials: 0.9 kg sea salt, 0.12 kg sodium hyaluronate, 0.03 kg lutein, 0.2 kg cassia seed extract, 0.3 kg wild chrysanthemum extract, 0.007 kg borneol, 0.01 kg hainan pea vine extract;

[0084] Step 4: Add lutein to a solution containing 20% ​​of the extract of Millettia hainanensis, stir at 42°C and 186r / min for 22min; cool to room temperature, add sodium hyaluronate, borneol and wild chrysanthemum extract mixed with cassia seed extract in sequence, and stir at 190r / min for 35min;

[0085] Step 5: Add sea salt solution to dilute to 0.03% lutein to obtain eye drops;

[0086] Step 6: Pour the eye drops into the spray bottle to get the eye spray.

[0087] Comparative Example 1: The difference between this comparative example and Example 3 is that: Step 1 is to prepare the wild chrysanthemum extract by water extraction;

[0088] This comparative example provides a preparation process of an eye spray, comprising the following steps:

[0089] Step 1: preparing wild chrysanthemum extract;

[0090] 1) Crushing: crush the wild chrysanthemum into 40 meshes by a crusher;

[0091] 2) Extracting wild chrysanthemum powder by water extraction;

[0092] 3) Filtration centrifugation: speed 6000r / min;

[0093] 4) Evaporation and concentration;

[0094] 5) Sterilization: Ultra-high temperature instantaneous sterilization is used, the sterilization temperature is 135°C, and the time is 28s;

[0095] 6) Low temperature drying: drying at 55°C to obtain the wild chrysanthemum extract;

[0096] Step 2: preparing the extract of Millettia hainanensis by subcritical extraction;

[0097] 1) Grind the hainan pea vine into a 20-mesh powder and completely immerse it in a 41°C sodium carbonate solution with a pH of 9 for 2 hours. Filter and dry the powder and repeat the alkali washing for 3 times.

[0098] 2) adding the alkali-washed Millettia hainanensis powder into a subcritical extraction kettle, using water as the extractant, the mass ratio of water to Millettia hainanensis powder being 9:1; the pressure being 0.7 MPa, the extraction temperature being 170°C, the extraction time being 24 min, and obtaining an extract;

[0099] 3) filtering to remove solid impurities to obtain an extract, and evaporating and concentrating the extract to obtain a Millettia hainanensis extract;

[0100] Step 3: weigh the raw materials: 0.9 kg sea salt, 0.12 kg sodium hyaluronate, 0.03 kg lutein, 0.2 kg cassia seed extract, 0.3 kg wild chrysanthemum extract, 0.007 kg borneol, 0.01 kg hainan pea vine extract;

[0101] Step 4: Add lutein to a solution containing 20% ​​of the extract of Millettia hainanensis, stir at 42°C and 186r / min for 22min; cool to room temperature, add sodium hyaluronate, borneol and wild chrysanthemum extract mixed with cassia seed extract in sequence, and stir at 190r / min for 35min;

[0102] Step 5: Add sea salt solution to dilute to 0.03% lutein to obtain eye drops;

[0103] Step 6: Pour the eye drops into the spray bottle to get the eye spray.

[0104] Comparative Example 2: This comparative example is different from Example 3 in that: no extract of Millettia hainanensis is added;

[0105] This comparative example provides a preparation process of an eye spray, comprising the following steps:

[0106] Step 1: preparing wild chrysanthemum extract;

[0107] 1) Crushing: crush the wild chrysanthemum into 40 meshes by a crusher;

[0108] 2) Low-temperature and ultra-high pressure synergistic pretreatment: The crushed wild chrysanthemum is placed in an immersion freezing device with -38℃ liquid alcohol as the freezing medium. When the center temperature of the crushed wild chrysanthemum reaches -4℃, the freezing is completed, and ultra-high pressure treatment is performed at 220Mpa for 2 minutes;

[0109] 3) Extraction: put the pretreated wild chrysanthemum into an extraction tank, then add a 60% ethanol solution with a weight of 10 times the weight of the raw material and a temperature of 41°C, add 0.3% cellulase based on the weight of the wild chrysanthemum, 0.2% pectinase based on the weight of the raw material, and 0.05% decarboxylase based on the weight of the raw material, respectively, and release the enzymatic extract after enzymolysis for 52 minutes;

[0110] 4) Filtration centrifugation: speed 6000r / min;

[0111] 5) Evaporation and concentration;

[0112] 6) Sterilization: Ultra-high temperature instantaneous sterilization is used, the sterilization temperature is 135°C, and the time is 28s;

[0113] 7) Low temperature drying: Drying at 55°C to obtain the wild chrysanthemum extract;

[0114] Step 2: weigh the raw materials: 0.91kg sea salt, 0.12kg sodium hyaluronate, 0.03kg lutein, 0.2kg cassia seed extract, 0.3kg wild chrysanthemum extract, and 0.007kg borneol;

[0115] Step 3: Add lutein into water and dissolve it, stir at 42°C and 186r / min for 22min; cool to room temperature, add sodium hyaluronate, borneol and wild chrysanthemum extract mixed with cassia seed extract in sequence, and stir at 190r / min for 35min;

[0116] Step 4: Add sea salt solution to dilute to 0.03% lutein to obtain eye drops;

[0117] Step 5: Pour the eye drops into the spray bottle to get the eye spray.

[0118] Comparative Example 3: This comparative example is different from Example 3 in that: in step 1, the wild chrysanthemum extract is prepared by water extraction, and the hainan pea vine extract is not added;

[0119] This comparative example provides a preparation process of an eye spray, comprising the following steps:

[0120] Step 1: preparing wild chrysanthemum extract;

[0121] 1) Crushing: crush the wild chrysanthemum into 40 meshes by a crusher;

[0122] 2) Extracting wild chrysanthemum powder by water extraction;

[0123] 3) Filtration centrifugation: speed 6000r / min;

[0124] 4) Evaporation and concentration;

[0125] 5) Sterilization: Ultra-high temperature instantaneous sterilization is used, the sterilization temperature is 135°C, and the time is 28s;

[0126] 6) Low temperature drying: drying at 55°C to obtain the wild chrysanthemum extract;

[0127] Step 2: weigh the raw materials: 0.91kg sea salt, 0.12kg sodium hyaluronate, 0.03kg lutein, 0.2kg cassia seed extract, 0.3kg wild chrysanthemum extract, and 0.007kg borneol;

[0128] Step 3: Add lutein into water and dissolve it, stir at 42°C and 186r / min for 22min; cool to room temperature, add sodium hyaluronate, borneol and wild chrysanthemum extract mixed with cassia seed extract in sequence, and stir at 190r / min for 35min;

[0129] Step 4: Add sea salt solution to dilute to 0.03% lutein to obtain eye drops;

[0130] Step 5: Pour the eye drops into the spray bottle to get the eye spray.

[0131] Experimental example

[0132] 1. Detection of the effect of improving eyesight and removing turbidity:

[0133] 50 rats were randomly divided into 5 groups, 10 rats in each group, namely group A, group B, group C and group D. 0.05% sodium selenite solution 0.01L / kg was injected into the peritoneum of the rats in each group for 3 consecutive days to establish the model; 3 days later, the spray prepared in Examples 1-3 and Comparative Examples 1-3 was sprayed into the eyeballs of the rats, 3 times a day, 4h apart, for 7 consecutive days, and then the lenses of the rats in each group were observed under a slit lamp. The observation results are as follows:

[0134] 2. Wettability test: The smaller the surface tension, the better the wettability (the surface tension of pure water is 72.5mN / m);

[0135] Using an optical measuring instrument at (25±0.1)℃, when a droplet with a maximum diameter de is injected using a syringe, the surface tension σ of the liquid is calculated by the LY equation. The surface tension value of each solution is calculated as the average of four measurements to reduce experimental errors. The calculation of the surface tension (mN / m) is as follows:

[0136]

[0137] Where de is the diameter of the droplet at its maximum; ds is the diameter above de; density is the density of water; g is the acceleration due to gravity;

[0138]

[0139] As can be seen from the above table, the combination of Hainan Millettia vine and lutein can effectively improve the effect of lutein in improving eye opacities; the low-temperature and ultra-high pressure synergistic pretreatment of wild chrysanthemum followed by extraction can effectively reduce the surface tension, that is, improve the eye-moisturizing effect; the combination of Hainan Millettia vine and wild chrysanthemum pretreatment methods not only significantly improves the eye-moisturizing effect and the effect of lutein in improving eye opacities, but also unexpectedly improves the effect of improving eye redness.

[0140] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. Such modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. An eye spray, characterized in that: The invention comprises a spray bottle and an eye-moistening liquid, wherein the eye-moistening liquid comprises the following raw materials: 0.8-1.1% sea salt, 0.1-0.15% sodium hyaluronate, 0.01-0.04% lutein, 0.02-0.4% cassia seed extract, 0.05-0.7% wild chrysanthemum extract, 0.005-0.009% borneol, 0.008-0.02% hainan pea vine extract, and the balance is water; The preparation method of wild chrysanthemum extract is as follows: 1) Crushing: crush the wild chrysanthemum into 20-40 meshes by a crusher; 2) Low-temperature and ultra-high pressure synergistic pretreatment: The crushed wild chrysanthemum is placed in an immersion freezing device with -40~-30℃ liquid alcohol as the freezing medium. When the center temperature of the crushed wild chrysanthemum reaches -5~-2℃, the freezing is completed and ultra-high pressure treatment is performed; 3) Extraction: put the pretreated wild chrysanthemum into the extraction tank, then add 60% ethanol solution with a weight of 9-12 times the weight of the raw material and a temperature of 40-45°C, add cellulase, pectinase and decarboxylase respectively, and release the enzymatic extract after enzymolysis for 50-60 minutes; 4) Filtration and centrifugation; 5) Evaporation and concentration; 6) Sterilization; 7) Low temperature drying to obtain wild chrysanthemum extract; The preparation method of the extract of Millettia hainanensis is as follows: 1) Grind the Hainan Millettia oleifera into powder and completely immerse it in a sodium carbonate solution at 38-46°C for 1-2 hours, filter and dry the powder, and repeat the alkali washing 2-3 times; 2) Add the alkali-washed Millettia hainanensis powder into a subcritical extraction kettle, use water as the extractant, and the mass ratio of water to Millettia hainanensis powder is 8-9:1 to obtain an extract; the subcritical extraction pressure is 0.6-0.7Mpa, the extraction temperature is 150-180℃, and the extraction time is 20-25min; 3) filtering to remove solid impurities to obtain an extract, and evaporating and concentrating the extract to obtain a Millettia hainanensis extract.

2. A process for preparing the eye spray according to claim 1, characterized in that: The following steps are involved: Step 1: preparing wild chrysanthemum extract; 1) Crushing: crush the wild chrysanthemum into 20-40 meshes by a crusher; 2) Low-temperature and ultra-high pressure synergistic pretreatment: The crushed wild chrysanthemum is placed in an immersion freezing device with -40~-30℃ liquid alcohol as the freezing medium. When the center temperature of the crushed wild chrysanthemum reaches -5~-2℃, the freezing is completed and ultra-high pressure treatment is performed; 3) Extraction: put the pretreated wild chrysanthemum into the extraction tank, then add 60% ethanol solution with a weight of 9-12 times the weight of the raw material and a temperature of 40-45°C, add cellulase, pectinase and decarboxylase respectively, and release the enzymatic extract after enzymolysis for 50-60 minutes; 4) Filtration and centrifugation; 5) Evaporation and concentration; 6) Sterilization; 7) Low temperature drying to obtain wild chrysanthemum extract; Step 2: preparing the extract of Millettia hainanensis by subcritical extraction; 1) Grind the Hainan Millettia oleifera into powder and completely immerse it in a sodium carbonate solution at 38-46°C for 1-2 hours, filter and dry the powder, and repeat the alkali washing 2-3 times; 2) Add the alkali-washed Millettia hainanensis powder into a subcritical extraction kettle, use water as the extractant, and the mass ratio of water to Millettia hainanensis powder is 8-9:1 to obtain an extract; the subcritical extraction pressure is 0.6-0.7Mpa, the extraction temperature is 150-180℃, and the extraction time is 20-25min; 3) filtering to remove solid impurities to obtain an extract, and evaporating and concentrating the extract to obtain a Millettia hainanensis extract; Step 3: Add lutein to the solution containing the Hainan Millettia oleifera extract, stir at 40-50°C; cool to room temperature, add sodium hyaluronate, borneol and wild chrysanthemum extract mixed with cassia seed extract in sequence, and stir for 30-40 minutes; Step 4: Add sea salt solution to dilute to 0.01-0.04% lutein to obtain eye drops; Step 5: Pour the eye drops into the spray bottle to get the eye spray.

3. The preparation process of the eye spray according to claim 2, characterized in that: In step one, step 2) is specifically as follows: placing the crushed wild chrysanthemum in an immersion freezing device with -40~-30°C liquid alcohol as a freezing medium, and the freezing is completed when the center temperature of the crushed wild chrysanthemum reaches -5~-2°C, and performing ultra-high pressure treatment at 200~250Mpa for 1~5min.

4. The preparation process of the eye spray according to claim 2, characterized in that: In step one, step 3) is specifically as follows: the pretreated wild chrysanthemum is placed in an extraction tank, and then a 60% ethanol solution with a weight of 9 to 12 times the weight of the raw material and a temperature of 40 to 45° C. is added, and 0.2 to 0.3% of cellulase, 0.2 to 0.3% of pectinase, and 0.05 to 0.1% of decarboxylase based on the weight of the wild chrysanthemum are added respectively, and after enzymolysis for 50 to 60 minutes, the enzymolysis extract is released.

5. The preparation process of the eye spray according to claim 2, characterized in that: In step 1, the centrifugal speed in step 4) is 6000~8000r / min.

6. The preparation process of the eye spray according to claim 2, characterized in that: In step 1, in step 6), the sterilization temperature is 135~137°C and the time is 25~30s.

7. The preparation process of the eye spray according to claim 2, characterized in that: In step 1, the drying temperature in step 7) is 40-60°C.

8. The preparation process of the eye spray according to claim 2, characterized in that: In step 2, the Hainan Millettia chinensis is ground into a powder of 10-40 mesh and then completely immersed in a sodium carbonate solution with a pH of 9-10 at 38-46° C. for alkali washing for 1-2 hours. The powder is filtered and dried and then the alkali washing is repeated 2-3 times.

9. The preparation process of the eye spray according to claim 2, characterized in that: Step three is as follows: add lutein to a solution containing 20-30% of Millettia hainanensis extract, stir at 40-50°C and 180-200r / min for 20-24min; cool to room temperature, add sodium hyaluronate, borneol and wild chrysanthemum extract mixed with cassia seed extract in sequence, and stir at 180-200r / min for 30-40min.

Images