Biological enzymolysis extraction process of alginic acid

Abstract

The invention discloses a biological enzymolysis extraction process of alginic acid, which comprises the following steps: taking 130kg-170kg of kelp, cleaning with deionized water to remove salt, then carrying out wet crushing with a pipeline scroll pump, adding the crushed kelp into a reaction kettle, then adding 750-850L of a desorber and 45-55L of a biological active enzyme, heating in an electric bath, then adding 45-55kg of monopotassium phosphate, keeping the reaction time for 8-12 hours, measuring the pH value of the solution, and filtering to obtain the alginic acid. Adjusting the pH value to 6.5-7.5 by using citric acid and potassium hydroxide, then heating to 80-90 DEG C, keeping for 1.5-2.5 hours, killing the activity of biological enzyme, and filtering by using a plate-and-frame pressure filter with the filtering area of 40m < 2 > to obtain an alginic acid solution containing 18-22% of alginic acid. According to the technical scheme, extraction equipment is simple, operability is high, production is easy, and alginic acid is rapidly extracted through biological enzymolysis.

Description

Technical Field

[0001] The invention belongs to the technical field of alginate extraction, and in particular relates to a biological enzymatic extraction process of alginate. Background Art

[0002] Alginate, with a molecular formula of (C6H7O6)n and a relative molecular weight of about 32,000 to 200,000, is a polysaccharide carbohydrate extracted from brown algae such as kelp or sargassum. In recent years, people have paid more and more attention to alginic acid due to its wide range of uses. Alginic acid is also used as a capsule in the pharmaceutical industry for its safety and no side effects. In the feed industry, alginic acid is also used as a binder and an attractant.

[0003] Effects and extraction methods of alginic acid (Guyu)

[0004] Pure alginic acid is white to brownish yellow fiber, granules or powder. Alginic acid easily forms gel with cations, such as sodium alginate, which is called alginate, algin or algin.

[0005] Efficacy: In recent years, new fertilizers containing alginic acid have emerged in an endless stream. In foreign countries, alginic acid has been used in agriculture for a long time. Seaweed used as fertilizer is generally large economic algae, such as giant kelp, ascophyllum, sea cyst, etc. The development of seaweed fertilizer has gone through three stages: rotten seaweed → seaweed ash (powder) → seaweed extract. The core substance in seaweed fertilizer is pure natural seaweed extract. The main raw material is selected from natural seaweed. After special biochemical process treatment, the essence of seaweed is extracted, which greatly retains the natural active components. It contains a large amount of non-nitrogenous organic matter, potassium, calcium, magnesium, zinc, iodine and more than 40 mineral elements and rich vitamins that are incomparable to terrestrial plants. In particular, it contains seaweed polysaccharides, alginate, highly unsaturated fatty acids and a variety of natural plant growth regulators that are unique to seaweed. It has high biological activity, can stimulate the production of non-specific active factors in plants, and regulate the balance of endogenous hormones.

[0006] Function: Alginate contains more than 40 mineral elements or beneficial elements such as potassium, calcium, magnesium, iron, manganese, zinc, iodine, etc.; it is also rich in amino acids, seaweed protein, vitamins, seaweed polyphenols, mannitol, biological enzymes, betaine and other plant growth stimulating substances; it also contains active conditioning ingredients such as auxins, cytokinins (kinetics), gibberellins, abscisic acid (inducing factor), ethylene, brassinolide, etc. Alginate can promote crop seed germination, root growth, flower bud differentiation, fruit enlargement, improve crop resistance to drought, cold and disease, fertilize soil, improve structure, reduce the harm of continuous cropping, promote the breeding of beneficial microorganisms, purify soil, and improve fertilizer utilization, reduce costs, optimize quality and increase yield through the comprehensive action of active substances.

[0007] Alginate is produced from seaweed (mainly brown algae, ascophyllum, and giant kelp) through industrial processing and extraction using physical, biochemical, and biotechnological processes to extract various active substances, mineral elements, and antibiotics that are beneficial to plants, creating a natural plant nutrient.

[0008] Therefore, the present invention proposes a composite biological enzymatic hydrolysis extraction process for alginate. Summary of the invention

[0009] The purpose of the present invention is to provide a biological enzymatic extraction process for alginate which can quickly extract alginate through biological enzymatic hydrolysis.

[0010] In order to achieve the above technical effects, the present invention is implemented by the following technical scheme: a biological enzymatic extraction process of alginic acid, characterized in that it comprises the following steps:

[0011] S1, taking 130kg to 170kg of giant kelp, washing and desalting with deionized water to obtain a desalted material;

[0012] S2, wet-crushing the desalted material using a pipeline coil pump to obtain a crushed material;

[0013] S3, adding the crushed material into a reactor, adding 750-850 L of deionized water into the reactor, adding 45-55 L of biologically active enzyme, heating the reactor to 50-55° C. by electric bath heating, adding 45-55 kg of 99% pure potassium dihydrogen phosphate (pretreatment), maintaining the reaction time for 8-12 hours, measuring the pH value of the solution, and adjusting the pH value to between 6.5 and 7.5 to obtain an original solution;

[0014] S4, heating the original solution in a reactor to 80-90° C. and maintaining the temperature for 1.5-2.5 hours to kill the activity of the biologically active enzymes and obtain a mixed solution;

[0015] S5, introduce the mixed solution into the filtration area of ​​35-45m 2 The product is filtered by a plate and frame pressure filter to obtain an alginate solution containing 18-22% alginate; the overall extraction rate reaches 80.3-82.1%.

[0016] Furthermore, the deionized water is resin-softened water with calcium and magnesium ions less than 0.3 mol / L.

[0017] Furthermore, the volume of the reactor is 1m 3 ~1.5m 3 .

[0018] Furthermore, the bioactive enzyme is a mixed solution of a cellulolytic enzyme solution and a proteolytic enzyme solution.

[0019] Furthermore, the pH value is adjusted by using citric acid with a purity of 90% for alkaline and potassium hydroxide with a purity of 95% for acid.

[0020] The beneficial effects of the present invention are:

[0021] The extraction equipment of the invention is simple, has strong operability and is easy to produce. Compared with the process of extracting alginic acid by using Sargassum through enzymatic hydrolysis technology, the extraction process of the invention is that the extraction rate of the sodium carbonate pretreatment method by enzymatic hydrolysis technology only reaches 42.8%; the extraction rate of the hydrochloric acid pretreatment method only reaches 22.8%; although the extraction rate of the papain pretreatment method reaches 94.28%, the preparation cost is too high; and the extraction rate of the potassium dihydrogen phosphate pretreatment method by the process of the invention reaches 82.1%, which is 39.3% higher than that of the sodium carbonate pretreatment method, 59.3% higher than that of the hydrochloric acid pretreatment method, and although the extraction rate is 12.18% lower than that of the papain pretreatment method, the cost is reduced by 32.7% compared with the papain pretreatment method. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for describing the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative work.

[0023] Figure 1 The present invention is a flowchart of the biological enzymatic extraction process of alginate. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] Example 1

[0026] See also Figure 1 As shown, a biological enzymatic extraction process of alginic acid is characterized in that it specifically includes the following steps:

[0027] S1, taking 150 kg of giant kelp, washing and desalting with resin softened water containing less than 0.3 mol / L of calcium and magnesium ions to obtain a desalted material;

[0028] S2. Wet-crushing the desalted material with a pipe coil pump to obtain a crushed material; the copolymer of lignin, hemicellulose and cellulose embedded with pectin in the cell wall of the giant algae can be broken up into a dispersion of lignin, pectin, hemicellulose and cellulose, thereby destroying the natural barrier system of lignin without damaging alginic acid;

[0029] S3. Add crushed materials into a volume of 1m 3 The reaction kettle is added with 800 L of resin softened water containing less than 0.3 mol / L calcium and magnesium ions and 50 L of biologically active enzymes, heated in an electric bath to 50-55° C., 50 kg of 99% pure potassium dihydrogen phosphate is added, the reaction time is maintained for 8-12 hours, the pH value of the solution is measured, and the pH value is adjusted by adding citric acid and potassium hydroxide to a pH value between 6.5 and 7.5 to obtain an original solution;

[0030] S4, heating the original solution in a reactor to 80-90° C. and maintaining the temperature for 2 hours to kill the activity of the biologically active enzymes, thereby obtaining a mixed solution;

[0031] S5, the mixed solution is introduced into a filter with a filtration area of ​​40m 2 Filtered by plate and frame pressure filter, 1m 3 The finished product of alginate solution containing 18-22% alginate.

[0032] In step S3, the volume used is 1m 3 The reactor can store all materials inside the reactor, avoiding the need to constantly change containers during preparation, reducing process complexity and improving preparation efficiency;

[0033] The biologically active enzyme in step S3 is a mixed solution of a cellulolytic enzyme solution and a proteolytic enzyme solution, which can degrade lignin, pectin, hemicellulose, chitin and chitosan into small molecules, break the cell structure of giant kelp, and reduce the interference of other macromolecular substances on the extraction of alginate in the preparation process of alginate and sodium alginate.

[0034] The pH value adjustment in step S3 is specifically to use citric acid with a purity of 90% to adjust the pH value if the pH value is alkaline, and to use potassium hydroxide with a purity of 95% to adjust the pH value if the pH value is acidic.

[0035] Example 2

[0036] Compared with the process of extracting alginic acid from Sargassum using enzymatic hydrolysis technology, the extraction rate of the acetolysis technology using sodium carbonate pretreatment is higher;

[0037] The acetolysis technology uses sodium carbonate pretreatment process, the steps are as follows:

[0038] Seaweed → extraction (sodium carbonate) → filtration → bleaching (sodium hypochlorite) acid precipitation (hydrochloric acid) → filtration → alginic acid;

[0039] 100 g of Sargassum powder was added to three containers respectively, and 500 ml of water was added thereto for dispersion, and the mixture was kept at 55° C. for 2 h to obtain three reagents;

[0040] 2g papain, 10g sodium carbonate and 10ml hydrochloric acid were added to three reagents respectively for pretreatment to obtain a mixed solution of alginic acid;

[0041] The extraction rates of alginate obtained by the three pretreatment methods were 42.86% and 22.86% respectively;

[0042] The extraction rate of the process of the present invention reaches 82.1%, which is 39.3% higher than that of the sodium carbonate pretreatment method and 59.3% higher than that of the hydrochloric acid pretreatment method; the extraction cost of the extraction process of the present invention is 32.7% lower than that of the papain treatment method. The product meets the GB1976-80 standard.

[0043] Example 3

[0044] The extraction cost of the extraction process of the present invention is 4150 for 1000L, which is 32.7% lower than the cost of 5500 for 1000L extraction by the existing process (papain treatment method).

Claims

1. A biological enzymatic extraction process of alginic acid, characterized in that: The following steps are involved: S1, taking 130kg to 170kg of giant kelp, washing and desalting with deionized water to obtain a desalted material; S2, wet-crushing the desalted material using a pipeline coil pump to obtain a crushed material; S3, adding the crushed material into a reactor, adding 750-850 L of deionized water into the reactor, adding 45-55 L of biologically active enzyme, heating the reactor in an electric bath to 50-55° C., adding 45-55 kg of 99% pure potassium dihydrogen phosphate, maintaining the reaction time for 8-12 hours, measuring the pH value of the solution, and adjusting the pH value to between 6.5 and 7.5 to obtain an original solution; S4, raising the temperature of the original solution to 80-90° C. in a reactor and maintaining the temperature for 1.5-2.5 hours to kill the activity of the biologically active enzymes and obtain a mixed solution; S5, introduce the mixed solution into the filtration area of ​​35-45m 2 The product is filtered through a plate and frame pressure filter to obtain a finished alginate solution containing 18-22% alginate.

2. The bio-enzymatic extraction process of alginic acid according to claim 1, characterized in that: The deionized water is resin softened water with calcium and magnesium ions less than 0.3 mol / L.

3. The bio-enzymatic extraction process of alginic acid according to claim 1, characterized in that: The volume of the reactor is 1m 3 ~1.5m 3 .

4. The bio-enzymatic extraction process of alginic acid according to claim 1, characterized in that: The biologically active enzyme is a mixed solution of a fiber decomposing enzyme solution and a protein decomposing enzyme solution.

5. The bio-enzymatic extraction process of alginic acid according to claim 1, characterized in that: The pH value is adjusted specifically by using citric acid with a purity of 90% for alkaline and potassium hydroxide with a purity of 95% for acid.

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