Enhanced linear colorimetric pool structure of biological detection chip
By designing the short-diameter and length-diameter colorimetric pore structure on the biological detection chip, the problem of poor repeatability of the detection results caused by weak optical signals of the POCT instrument is solved, and linear and complete detection results are achieved without additional costs.
Patent Information
- Application Number
- CN202411526457.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-30
- Publication Date
- 2024-12-27
AI Technical Summary
The light signal intensity of existing POCT instant inspection instruments is weak, resulting in poor repeatability of detection results for high concentration or weak positive samples, and signal amplification methods will lead to increased light noise and reduced resolution.
An enhanced linear colorimetric pool structure of a biodetection chip is designed, including short-diameter and length-diameter colorimetric holes. The connection and height difference between the colorimetric holes and the reaction chamber are used to treat the height and low respectively using the short-diameter and length-diameter colorimetric holes. Concentration sample, achieving linear enhancement of the signal.
Using conventional small photoelectric acquisition modules, linear and complete detection results can be obtained, and satisfactory application results are achieved without increasing the cost of the instrument.
Smart Images
Figure CN119198584A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of centrifugal microfluidic chips, and in particular relates to an enhanced linear colorimetric cell structure of a biological detection chip. Background Art
[0002] In the prior art, POCT instant testing instruments are generally small, with a total weight of less than 10KG and a small space inside the machine. Although there is no pipetting system and cleaning system, it is also impossible to install a high-power light source and a large-size color separation signal acquisition device like an ordinary biochemical analyzer. This causes the optical signal intensity of the POCT instant testing instrument to be weaker than that of an ordinary biochemical detection instrument. At present, the reaction chambers of microfluidic chips are mostly cylindrical with a fixed volume. The detection liquid will fill the entire reaction chamber. The weak optical signal will cause the ability to detect samples with higher concentrations to be weakened, resulting in a serious problem of being unable to measure values. For this reason, existing POCT detectors generally use a method of amplifying signal intensity. Signal intensity amplification will cause problems such as increased optical noise and reduced resolution, weak positive detection specimens will become weaker, and the repeatability of the test results will be poor. Summary of the invention
[0003] 1. Technical problems to be solved
[0004] In view of the deficiencies of the prior art, the present invention provides an enhanced linear colorimetric cell structure for a biological detection chip, which solves the problem of poor relevance of detection results for high-concentration or weakly positive samples mentioned in the above background technology.
[0005] (2) Technical solution
[0006] To achieve the above objectives, the present invention is implemented through the following technical solutions: an enhanced linear colorimetric cell structure of a biological detection chip, including a reaction chamber, a reaction reagent inlet, a sample mixture inlet and a colorimetric hole, characterized in that the above-mentioned colorimetric hole includes a short-diameter colorimetric hole and a long-diameter colorimetric hole.
[0007] Preferably, the colorimetric hole is communicated with the reaction chamber.
[0008] Preferably, the short diameter ratio color hole is connected to the long diameter ratio groove hole.
[0009] Preferably, there is a height difference between the bottom of the short diameter colorimetric hole and the bottom of the long diameter colorimetric hole.
[0010] Preferably, the optical path length of the short-diameter colorimetric hole is 2.5 mm, and the optical path length of the long-diameter colorimetric hole is 5 mm.
[0011] Preferably, there are one or more reaction reagent inlets.
[0012] Preferably, the reaction reagent inlet is communicated with the reaction chamber.
[0013] The present invention provides an enhanced linear colorimetric cell structure for a biological detection chip, which has the following beneficial effects: 1. A detection result with complete linearity and high precision can be obtained by using a conventional small-scale photoelectric acquisition module.
[0014] 2. By improving the chip structure, satisfactory application effects can be achieved without increasing the cost of the instrument. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] Figure 1 For the present invention Figure 1 .
[0016] Figure 2 For the present invention Figure 2 .
[0017] In the figure: 1, reaction chamber; 11, reaction reagent inlet; 3, sample mixture inlet; 4, colorimetric hole; 41, short diameter colorimetric hole; 42, long diameter colorimetric hole. DETAILED DESCRIPTION
[0018] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0019] See also Figure 1-Figure 2 The present invention provides an enhanced linear colorimetric cell structure of a biological detection chip, comprising a reaction chamber, a reaction reagent inlet, a sample mixture inlet and a colorimetric hole, wherein the colorimetric hole comprises a short-diameter colorimetric hole and a long-diameter colorimetric hole.
[0020] Preferably, the colorimetric hole is communicated with the reaction chamber.
[0021] Preferably, the short diameter ratio color hole is connected to the long diameter ratio groove hole.
[0022] Preferably, there is a height difference between the bottom of the short diameter colorimetric hole and the bottom of the long diameter colorimetric hole.
[0023] Preferably, the optical path length of the short-diameter colorimetric hole is 2.5 mm, and the optical path length of the long-diameter colorimetric hole is 5 mm.
[0024] Preferably, there are one or more reaction reagent inlets.
[0025] Preferably, the reaction reagent inlet is communicated with the reaction chamber.
[0026] When the matching instrument tests a detection item, two sets of values will be obtained through the long and short colorimetric holes. Through comparative testing, the new instrument calibrates each detection item to obtain a threshold value. The samples with concentrations higher than this threshold shall be based on the test results of the long light distance colorimetric hole, and the standards with concentrations lower than this threshold shall be based on the test records of the short light distance colorimetric hole. The two sets of results can thus be synthesized into a test result with a sufficiently wide linear range and accurate and reliable performance.
[0027] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. An enhanced linear colorimetric cell structure of a biological detection chip, comprising a reaction chamber (1), a reaction reagent inlet (2), a sample mixed solution inlet (3) and a colorimetric hole (4), characterized in that: The colorimetric hole (4) comprises a short diameter colorimetric hole (41) and a long diameter colorimetric hole (42).
2. The enhanced linear colorimetric cell structure of a biological detection chip according to claim 1, characterized in that: The colorimetric hole (4) is in communication with the reaction chamber (1).
3. The enhanced linear colorimetric cell structure of a biological detection chip according to claim 1, characterized in that: The short-diameter colorimetric hole (41) is connected to the long-diameter ratio groove hole (42).
4. The enhanced linear colorimetric cell structure of a biological detection chip according to claim 3, characterized in that: There is a height difference between the bottoms of the short-diameter colorimetric hole (41) and the long-diameter colorimetric hole (42).
5. The enhanced linear colorimetric cell structure of a biological detection chip according to claim 1 or 4, characterized in that: The optical path length of the short-diameter colorimetric hole (41) is 2.5 mm, and the optical path length of the long-diameter colorimetric hole (42) is 5 mm.
6. The enhanced linear colorimetric cell structure of a biological detection chip according to claim 1, characterized in that: The reaction reagent inlet (2) has one or more.
7. The enhanced linear colorimetric cell structure of a biological detection chip according to claim 1 or 6, characterized in that: The reaction reagent inlet (2) is in communication with the reaction chamber (1).