Micro-fluidic chip for detection equipment and detection equipment

Abstract

The invention provides a micro-fluidic chip for detection equipment and the detection equipment, the micro-fluidic chip comprises a base body and a controller, the base body is provided with a sample cavity, a liquid storage module, a washing module, an elution cavity, a uniform mixing cavity, a storage cavity, a waste liquid cavity and an amplification module, and the controller is configured to control a driving part to drive the base body to execute first centrifugal operation, a sample, a reagent and a washing liquid are thrown into the uniform mixing cavity in batches, and waste liquid generated in the uniform mixing cavity in batches is thrown into the waste liquid cavity; the driving part is controlled to drive the base body to execute second centrifugal operation, so that the eluent is thrown into the containing cavity and the uniform mixing cavity in sequence; controlling the driving part to drive the base body to execute third centrifugal operation, so as to throw the treated detection liquid into the amplification module to be mixed with the solid reagent and generate amplification reaction liquid; and controlling the detection module to detect the amplification reaction liquid. The micro-fluidic chip for the detection equipment and the detection equipment are small and exquisite in structure and high in integration, and the stability and the accuracy of a nucleic acid detection structure are improved.

Description

Technical Field

[0001] The present invention relates to the field of microfluidic technology, and in particular to a microfluidic chip for a detection device and a detection device. Background Art

[0002] Microfluidic chips can complete the sample preparation, separation, purification, and detection processes of traditional laboratories on an inch-sized operating platform. They have the advantages of small size, high integration, fast analysis speed, and low reagent consumption. They are widely used in biomedicine, environmental monitoring, analytical chemistry and other fields. They can accurately and intuitively control fluids by using physical forces such as centrifugal force, Coriolis force, and Euler force. Microfluidic chips achieve separation, transportation, metering, mixing, diversion and other functions by setting up multiple interconnected chambers (such as sample chambers, mixing chambers, and detection chambers, etc.) and valves between chambers (such as capillary valves, siphon valves, and active valves, etc.). They are widely used in biomedicine, environmental monitoring, analytical chemistry and other fields. In the prior art, microfluidic chips include single-flux microfluidic chips and multi-flux microfluidic chips. Among them, the flow channel and control method of the single-flux microfluidic chip are relatively simple, and the production cost is also low, but its detection efficiency is also low; the flow channel and control method of the existing multi-flux microfluidic chip are relatively complex, and there may be cross-mixing problems in the reagent chamber, which will reduce the accuracy of the test results. In addition, the functions realized by the existing multi-flux microfluidic chip (such as CN113322172A) are basically limited to automated amplification and detection after nucleic acid extraction, and the sample pretreatment, that is, the nucleic acid extraction process, is not implemented on the chip. It is necessary to use other equipment to extract and process the sample. After the processing, the extracted sample is injected into the chip for detection. The process is cumbersome and requires professional operation, which will also affect the stability of the test results. Summary of the invention

[0003] The purpose of the embodiments of the present invention is to provide a microfluidic chip and a detection device for a detection device, which have the advantages of compact structure and high integration, and are conducive to improving the stability and accuracy of the nucleic acid detection structure.

[0004] In order to achieve the above-mentioned object, the first aspect of the present invention provides a microfluidic chip for a detection device, the detection device comprising a microfluidic chip, a driving member for driving the microfluidic chip to rotate, and a detection module, the microfluidic chip comprising:

[0005] A substrate, on which a sample chamber for accommodating a sample, a liquid storage module, a washing module, an elution chamber, a mixing chamber, a storage chamber, a waste liquid chamber and an amplification module are provided, wherein a reagent for lysing the sample is pre-stored in the liquid storage module, a washing liquid is pre-stored in the washing module, an elution liquid is pre-stored in the elution chamber, and a solid reagent is pre-stored in the amplification module; the sample chamber, the liquid storage module and the washing module are all connected to the inlet end of the mixing chamber, the waste liquid chamber is located at the downstream end of the mixing chamber, the storage chamber is located between the mixing chamber and the waste liquid chamber and communicates with the mixing chamber and the waste liquid chamber, the elution chamber is connected to the inlet end of the storage chamber, and the amplification module is connected to the outlet end of the mixing chamber;

[0006] The controller is connected to the drive and the detection module and is configured as follows:

[0007] Controlling the driving member to drive the base body to perform a first centrifugal operation, so as to throw the sample, reagent and washing liquid into the mixing chamber in batches, and throw the waste liquid generated in the mixing chamber in batches into the waste liquid chamber;

[0008] The control driving member drives the base body to perform a second centrifugal operation to throw the eluent into the receiving

[0009] cavity and mixing cavity;

[0010] Controlling the driving member to drive the base body to perform a third centrifugal operation, so as to throw the treated detection liquid into the amplification module to mix with the solid reagent and generate an amplification reaction liquid;

[0011] The control detection module detects the amplification reaction solution.

[0012] In an embodiment of the present invention, the sample chamber, the liquid storage module and the washing module are distributed at intervals along the circumference of the substrate on the substrate, a capillary valve is provided between the sample chamber, the liquid storage module and the mixing chamber, a first siphon valve is provided between the washing module and the mixing chamber, the liquid storage module includes a plurality of liquid storage chambers distributed at intervals along the radial direction of the substrate, the washing module includes a plurality of washing chambers distributed at intervals along the circumference of the substrate, and controlling the driving member to drive the substrate to perform a first centrifugal operation to throw the sample, the reagent and the washing solution into the mixing chamber in batches includes:

[0013] Controlling the driving member to drive the base to perform a first rotation operation in a first centrifugal operation, so that the sample is thrown into the mixing chamber under the action of the first centrifugal force;

[0014] Controlling the driving member to drive the base body to perform a second rotation operation in the first centrifugal operation, so that the reagents in each liquid storage chamber are thrown into the mixing chamber in batches under the action of the second centrifugal force;

[0015] The driving member is controlled to drive the base body to perform the third rotation operation in the first centrifugal operation, so that the washing liquid in each washing chamber is thrown into the mixing chamber in batches under the action of the third centrifugal force.

[0016] In an embodiment of the present invention, the plurality of liquid storage chambers include a first liquid storage chamber, a second liquid storage chamber, and a third liquid storage chamber, and controlling the driving member to drive the base to perform a second rotation operation in the first centrifugal operation so that the reagents in each liquid storage chamber are thrown into the mixing chamber in batches under the action of the second centrifugal force includes:

[0017] The control driving member drives the base to perform a second rotation operation. The second rotation operation process includes a first rotation stage, a second rotation stage and a third rotation stage with different second centrifugal forces. The reagent in the first liquid storage chamber is thrown into the mixing chamber in the first rotation stage, the reagent in the second liquid storage chamber is thrown into the mixing chamber in the second rotation stage, and the reagent in the third liquid storage chamber is thrown into the mixing chamber in the third rotation stage.

[0018] In an embodiment of the present invention, the microfluidic chip further comprises a first heating element disposed below the mixing chamber and communicatively connected to the controller, and the controller is further configured to:

[0019] The first heating element is controlled to heat the mixing chamber at a preset temperature for a first preset time period between the second rotation stage and the third rotation stage.

[0020] In an embodiment of the present invention, the plurality of washing chambers include a first washing chamber, a second washing chamber and a third washing chamber, a first connecting channel is provided between the first washing chamber and the second washing chamber, a first paraffin valve is provided on the first connecting channel, a second connecting channel is provided between the second washing chamber and the third washing chamber, a second paraffin valve is provided on the second connecting channel, the microfluidic chip further includes a second heating element and a third heating element respectively arranged below the first connecting channel and the second connecting channel, the third rotation operation process includes a fourth rotation stage, a fifth rotation stage and a sixth rotation stage, and controlling the driving element to drive the substrate to perform the third rotation operation in the first centrifugal operation so that the washing liquid in each washing chamber is thrown into the mixing chamber in batches under the action of the third centrifugal force, including:

[0021] The driving member is controlled to drive the base body to perform a rotation operation corresponding to the fourth rotation stage, and the washing liquid in the first washing chamber is thrown into the mixing chamber;

[0022] Controlling the second heating element to heat so as to conduct the first connecting channel;

[0023] The driving member is controlled to drive the base body to perform a rotation operation corresponding to the fifth rotation stage, and the washing liquid in the second washing chamber is thrown into the mixing chamber;

[0024] Controlling the third heating element to heat so as to conduct the second connecting channel;

[0025] The driving member is controlled to drive the base body to perform a rotation operation corresponding to the sixth rotation stage, and the washing liquid in the third washing chamber is thrown into the mixing chamber.

[0026] In an embodiment of the present invention, the first centrifugation operation further includes a lysis waste liquid discharge operation and a washing waste liquid discharge operation, and throwing the waste liquid generated in batches in the mixing chamber into the waste liquid chamber includes:

[0027] After the third rotation stage is completed, the driving member is controlled to drive the base body to perform a washing waste liquid discharge operation, so as to throw the waste liquid generated by sample lysis in the mixing chamber into the waste liquid chamber;

[0028] During the third rotation operation, the driving member is controlled to drive the base body to perform the washing waste liquid discharge operation multiple times at intervals, so as to throw the waste liquid generated in batches due to washing of the target substance in the mixing chamber into the waste liquid chamber.

[0029] In an embodiment of the present invention, the controller is further configured to:

[0030] After the first centrifugation operation is completed and before the second centrifugation operation, the first heating element is controlled to heat the mixing chamber to evaporate the residual waste liquid in the mixing chamber.

[0031] In an embodiment of the present invention, a third connecting channel is provided between the mixing chamber and the amplification module, a third paraffin valve is provided on the third connecting channel, a fourth heating element is provided below the third paraffin valve, and the controller is further configured as follows:

[0032] After the eluent is thrown into the mixing chamber, the fourth heating element is controlled to heat to conduct the third connecting channel;

[0033] After the third connecting channel is opened, the driving member is controlled to drive the base body to perform a third centrifugal operation.

[0034] In an embodiment of the present invention, the amplification module includes a detection chamber in which a solid reagent is pre-stored and an amplification chamber located at the downstream end of the detection chamber, a paraffin chamber and a fifth heating element located below the paraffin chamber are further provided on the substrate, the paraffin chamber is connected to the third connecting channel, and the controller is further configured as follows:

[0035] After the third centrifugal operation is completed, the driving member is controlled to drive the base body to perform a fourth centrifugal operation, so that the amplification reaction liquid in the detection chamber is thrown into the amplification chamber;

[0036] After the fourth centrifugation operation is completed, the fifth heating element is controlled to heat so that the paraffin pre-stored in the paraffin chamber is melted and the amplification module is sealed.

[0037] A second aspect of the present invention provides a detection device, which includes the above-mentioned microfluidic chip for detection equipment, a driving member for driving the above-mentioned microfluidic chip to rotate, and a detection module.

[0038] It can be known from the above technical scheme that the detection device includes a microfluidic chip, a driving member for driving the microfluidic chip to rotate, and a detection module. The microfluidic chip includes a substrate and a controller. The substrate is provided with a sample chamber for accommodating samples, a liquid storage module, a washing module, an elution chamber, a mixing chamber, a storage chamber, a waste liquid chamber and an amplification module. The controller and the driving member and the detection module are communicatively connected and configured to: control the driving member to drive the substrate to perform a first centrifugal operation to throw the sample, reagent and washing liquid into the mixing chamber in batches, and throw the waste liquid generated in the mixing chamber in batches into the waste liquid chamber; control the driving member to drive the substrate to perform a second centrifugal operation to throw the elution liquid into the storage chamber and the mixing chamber in turn; control the driving member to drive the substrate to perform a third centrifugal operation to throw the treated detection liquid into the amplification module to mix with the solid reagent and produce an amplification reaction liquid; control the detection module to detect the amplification reaction liquid. The microfluidic chip has a simple structure and a reasonable layout, and realizes the miniaturization and lightweight design of the microfluidic chip. When the detection personnel use the above-mentioned microfluidic chip to detect the target substance, they only need to inject the sample into the sample cavity and then install the microfluidic chip. The controller drives the matrix to automatically perform the centrifugation operation and detect the target substance in the sample. The operation is simple and reduces the labor intensity of the detection personnel, which is beneficial to improving the efficiency of the detection and ensuring the stability and accuracy of the detection results.

[0039] Other features and advantages of the embodiments of the present invention will be described in detail in the subsequent detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] The accompanying drawings are used to provide a further understanding of the embodiments of the present invention and constitute a part of the specification. Together with the following specific implementations, they are used to explain the embodiments of the present invention, but do not constitute a limitation on the embodiments of the present invention. In the accompanying drawings:

[0041] Figure 1 is a schematic diagram of a bottom-up perspective of a base body in an embodiment of the present invention;

[0042] Figure 2 is a schematic structural diagram of a liquid storage module in an embodiment of the present invention;

[0043] Figure 3 is a schematic structural diagram of a washing module in an embodiment of the present invention;

[0044] Figure 4 Schematic diagram of the overall structure of the microfluidic chip in an embodiment of the present invention;

[0045] Figure 5 2 is a schematic structural diagram of a column valve in an embodiment of the present invention;

[0046] Figure 6 It is the main control flow chart of the microfluidic chip in the embodiment of the present invention.

[0047] Description of Reference Numerals

[0048] 1-matrix; 101-sample chamber; 102-liquid storage module; 1021-first liquid storage chamber; 1022-second liquid storage chamber; 1023-third liquid storage chamber; 1024-fourth liquid storage chamber; 103-washing module; 1031-first washing chamber; 1032-second washing chamber; 1033-third washing chamber; 104-elution chamber; 105-mixing chamber; 106-storage chamber; 107-waste liquid chamber; 108-amplification module; 1081-detection chamber; 1082-amplification chamber; 109-first siphon valve; 110-first connecting channel; 111-first paraffin valve; 112-second connecting channel; 113-second paraffin valve; 114-third connecting channel; 115-third paraffin valve; 116-paraffin chamber; 117-cover; 118-first capillary channel; 119-second capillary channel; 120-third capillary channel; 121-fourth capillary channel; 122-fifth capillary channel; 123-sixth capillary channel; 124-fourth washing chamber; 125-fourth connecting channel; 126-fourth paraffin valve; 127-second siphon valve; 128-column valve; 129-seventh capillary channel; 130-fifth paraffin valve; 2-bottom plate. DETAILED DESCRIPTION

[0049] The specific implementation of the embodiment of the present invention is described in detail below in conjunction with the accompanying drawings. It should be understood that the specific implementation described here is only used to illustrate and explain the embodiment of the present invention, and is not used to limit the embodiment of the present invention.

[0050] The embodiment of the present invention provides a novel microfluidic chip for a detection device, wherein the detection device (such as a multiplex PCR nucleic acid detector) is used to detect nucleic acids (i.e., the target substance in this embodiment is nucleic acid) and comprises a microfluidic chip, a driving member for driving the microfluidic chip to rotate, and a detection module, such as Figure 1-5 As shown, the microfluidic chip includes:

[0051] A substrate 1, on which a sample chamber 101, a liquid storage module 102, a washing module 103, an elution chamber 104, a mixing chamber 105, a storage chamber 106, a waste liquid chamber 107 and an amplification module 108 for accommodating a sample are provided, wherein a reagent for lysing the sample is pre-stored in the liquid storage module 102, a washing liquid is pre-stored in the washing module 103, an elution liquid is pre-stored in the elution chamber 104, and a solid reagent is pre-stored in the amplification module 108; the sample chamber 101, the liquid storage module 102 and the washing module 103 are all connected to the inlet end of the mixing chamber 105; the waste liquid chamber 107 is located at the downstream end of the mixing chamber 105; the storage chamber 106 is located between the mixing chamber 105 and the waste liquid chamber 107 and connects the mixing chamber 105 and the waste liquid chamber 107; the elution chamber 104 is connected to the inlet end of the storage chamber 106; and the amplification module 108 is connected to the outlet end of the mixing chamber 105;

[0052] The controller is connected to the drive element and the detection module and is configured to execute the following steps: Figure 6 The steps shown, where:

[0053] Step S101: controlling the driving member to drive the base 1 to perform a first centrifugal operation, so as to throw the sample, reagent and washing liquid into the mixing chamber 105 in batches, and throw the waste liquid generated in the mixing chamber 105 in batches into the waste liquid chamber.

[0054] Specifically, the microfluidic chip in this embodiment is a centrifugal microfluidic chip, the substrate 1 is annular and has a central circular hole for mounting and cooperating with the driving end of the driving member, the center of the central circular hole is the rotation center of the microfluidic chip, and the driving member can be selected as a motor; the microfluidic chip also includes a base plate 2 arranged under the substrate 1 and a film for covering each cavity and microchannel on the substrate 1. Under the driving action of the driving member, the microfluidic chip as a whole can rotate around the rotation center.

[0055] The microfluidic chip also includes a cover 117 detachably arranged on the sample chamber 101. When nucleic acid detection is required for the sample, the detection personnel opens the cover 117 and adds the sample of the first preset capacity into the sample chamber 101, and then installs the microfluidic chip on the installation position of the detection device (the installation position is connected to the driving end of the driving member), wherein, in this embodiment, the first preset capacity is preferably 200ul (if the capacity of the sample is less than the first preset capacity, buffer GA can be added to make up for it); thereafter, the detection device is started again, and the controller can automatically execute the pre-stored program inside it to control the driving member and the detection module.

[0056] In one embodiment of the present invention, the sample chamber 101, the liquid storage module 102 and the washing module 103 are distributed on the substrate 1 along the circumferential direction of the substrate 1, a capillary valve is provided between the sample chamber 101, the liquid storage module 102 and the mixing chamber 105, a first siphon valve 109 is provided between the washing module 103 and the mixing chamber 105, the liquid storage module 102 includes a plurality of liquid storage chambers distributed along the radial direction of the substrate 1, and the washing module 103 includes a plurality of washing chambers distributed along the circumferential direction of the substrate 1, and step S101: controlling the driving member to drive the substrate 1 to perform a first centrifugal operation to throw the sample, the reagent and the washing solution into the mixing chamber 105 in batches includes steps S201-S203, wherein:

[0057] Step S201: controlling the driving member to drive the base 1 to perform a first rotation operation in a first centrifugal operation, so that the sample is thrown into the mixing chamber 105 under the action of the first centrifugal force.

[0058] Specifically, in the radial direction of the substrate 1, the mixing chamber 105 is located outside the sample chamber 101, and the sample chamber 101 and the mixing chamber 105 are connected by a first capillary channel 118 (the capillary channel is a microfluidic channel), and the first capillary channel 118 is a capillary valve. Under the driving action of the driving member, the substrate 1 performs a first rotation operation. At this time, although the liquids in the sample chamber 101, each chamber of the liquid storage module 102, and each chamber of the washing module 103 on the substrate 1 all generate centrifugal force and perform centrifugal motion, only the sample in the sample chamber 101 (the sample is in a liquid state) generates the first centrifugal force due to the first rotation operation (the magnitude of the centrifugal force generated by the liquid in other chambers is inconsistent with the magnitude of the first centrifugal force). When the first centrifugal force is greater than the capillary force generated by the first capillary channel 118, the sample can flow into the mixing chamber 105 through the first capillary channel 118.

[0059] Step S202: controlling the driving member to drive the base 1 to perform the second rotation operation in the first centrifugal operation, so that the reagents in each liquid storage chamber are thrown into the mixing chamber 105 in batches under the action of the second centrifugal force.

[0060] In one embodiment of the present invention, the plurality of liquid storage chambers include a first liquid storage chamber 1021, a second liquid storage chamber 1022, and a third liquid storage chamber 1023. Step S202: controlling the driving member to drive the base 1 to perform a second rotation operation in the first centrifugal operation, so that the reagents in each liquid storage chamber are thrown into the mixing chamber 105 in batches under the action of the second centrifugal force, specifically includes the following steps:

[0061] The control driving member drives the base 1 to perform a second rotation operation. The second rotation operation process includes a first rotation stage, a second rotation stage and a third rotation stage with different second centrifugal forces. The reagent in the first liquid storage chamber 1021 is thrown into the mixing chamber 105 in the first rotation stage, the reagent in the second liquid storage chamber 1022 is thrown into the mixing chamber 105 in the second rotation stage, and the reagent in the third liquid storage chamber 1023 is thrown into the mixing chamber 105 in the third rotation stage.

[0062] Specifically, the first liquid storage chamber 1021, the second liquid storage chamber 1022 and the third liquid storage chamber 1023 are spaced apart in the radial direction of the substrate 1, and the third liquid storage chamber 1023 is located on the outside of the first liquid storage chamber 1021, the second liquid storage chamber 1022 is located on the outside of the third liquid storage chamber 1023, and the mixing chamber 105 is located on the outside of the third liquid storage chamber 1023. The first liquid storage chamber 1021, the second liquid storage chamber 1022 and the third liquid storage chamber 1023 are respectively connected to the mixing chamber 105 through the second capillary channel 119, the third capillary channel 120 and the fourth capillary channel 121 which converge to the first capillary channel 118, and the second capillary channel 119, the third capillary channel 120 and the fourth capillary channel 121 are all capillary channels. The fine valve, the first liquid storage chamber 1021, the second liquid storage chamber 1022 and the third liquid storage chamber 1023 are pre-stored with reagents of different types and all used to lyse the sample, wherein the first liquid storage chamber 1021 is pre-stored with a second preset capacity of a first lysing reagent, the second liquid storage chamber 1022 is pre-stored with a third preset capacity of a second lysing reagent, and the third liquid storage chamber 1023 is pre-stored with a fourth preset capacity of a third lysing reagent. In this embodiment, the second preset capacity is preferably 20ul, the first lysing reagent is preferably proteinase K, the third preset capacity is preferably 200ul, the second lysing reagent is preferably buffer GB, the fourth preset capacity is preferably 200ul, and the third lysing reagent is preferably anhydrous ethanol.

[0063] The second rotation operation of the substrate 1 includes three rotation stages: the first rotation stage, the second rotation stage and the third rotation stage. The rotation direction of the substrate 1 in the above three rotation stages is counterclockwise. The centrifugal force generated by the liquid in the above liquid storage chamber in different rotation stages is called the second centrifugal force, but the magnitude of the second centrifugal force in different rotation stages is different. Specifically, in the first rotation stage, the second centrifugal force generated by 20ul proteinase K in the first liquid storage chamber 1021 is greater than the capillary force generated by the second capillary channel 119. At this time, 20ul proteinase K can flow through the second capillary channel 119 and part of the first capillary channel 118. into the mixing chamber 105; in the second rotation stage, the second centrifugal force generated by 200ul buffer GB in the second liquid storage chamber 1022 is greater than the capillary force generated by the third capillary channel 120, and at this time, 200ul buffer GB can flow into the mixing chamber 105 through the third capillary channel 120 and part of the first capillary channel 118; in the third rotation stage, the second centrifugal force generated by 200ul anhydrous ethanol in the third liquid storage chamber 1023 is greater than the capillary force generated by the fourth capillary channel 121, and at this time, 200ul anhydrous ethanol can flow into the mixing chamber 105 through the fourth capillary channel 121 and part of the first capillary channel 118.

[0064] In one embodiment of the present invention, the microfluidic chip further includes a first heating element disposed below the mixing chamber 105 and in communication with the controller, and the controller is further configured to perform step S2021:

[0065] Step S2021: controlling the first heating element to heat the mixing chamber 105 at a preset temperature for a first preset time period between the second rotation stage and the third rotation stage.

[0066] Specifically, the second rotation operation process further includes a fourth rotation stage. In the fourth rotation stage, after the second rotation stage is completed and before the first heating element is heated, the controller is further configured to perform step S2020, wherein:

[0067] Step S2020 : controlling the driving member to drive the base 1 to perform a rotation operation corresponding to the fourth rotation stage, so as to fully mix the sample with the reagent in the first liquid storage chamber 1021 and the reagent in the second liquid storage chamber 1022 .

[0068] That is, after the sample, proteinase K and buffer GB enter the mixing chamber 105, the controller controls the matrix 1 to reduce the rotation speed (the rotation speed at this time is not enough to break through the capillary force of each capillary valve), and repeatedly changes the rotation direction in the fourth rotation stage, so that the above three liquids are fully mixed in the mixing chamber 105, so that the sample can fully react with proteinase K and buffer GB, laying the foundation for the subsequent sample lysis.

[0069] After step S2020 is completed, the controller executes step S2021. In this embodiment, the rotation direction of the substrate 1 in step S2021 is counterclockwise. Furthermore, the preset temperature in this embodiment is preferably 70° C., and the first preset time is preferably 10 minutes, so that the cells in the sample can be fully lysed and release nucleic acids.

[0070] After step S2021 is completed, the controller controls the substrate 1 to perform a rotation operation in the third rotation stage, and throws 200ul of anhydrous ethanol into the mixing chamber 105. Anhydrous ethanol can clean the nucleic acid, and other impurities generated by the sample lysis during the cleaning process can be dissolved in anhydrous ethanol; further, after the anhydrous ethanol is thrown into the mixing chamber 105, the controller controls the substrate 1 to be repeatedly centrifuged for a second preset time (such as 15s) so that the impurities are fully dissolved in anhydrous ethanol; in addition, waste liquid is generated during the sample lysis process. In order to avoid affecting the subsequent test results, the above waste liquid should be discharged before performing other subsequent steps.

[0071] Furthermore, in the present embodiment, the liquid storage module 102 also includes a fourth liquid storage chamber 1024 located between the first liquid storage chamber 1021 and the third liquid storage chamber 1023 in the radial direction of the substrate 1, and the fourth liquid storage chamber 1024 is connected to the mixing chamber 105 through a fifth capillary channel 122, one end of the fifth capillary channel 122 is connected to the fourth liquid storage chamber 1024, and the other end of the fifth capillary channel 122 is connected to the first capillary channel 118; with different sample types, the type of lysis reagent pre-stored in the fourth liquid storage chamber 1024 is also different. Before nucleic acid testing, the testing personnel can select a microfluidic chip with a corresponding lysis reagent pre-stored in the fourth liquid storage chamber 1024 for nucleic acid testing according to the type of the sample, and the controller controls the substrate 1 to throw the lysis reagent pre-stored in the fourth liquid storage chamber 1024 into the mixing chamber 105 at an appropriate stage in the first centrifugation operation to react with the sample to promote the lysis of the sample.

[0072] Step S203: controlling the driving member to drive the base body 1 to perform the third rotation operation in the first centrifugal operation, so that the washing liquid in each washing chamber is thrown into the mixing chamber 105 in batches under the action of the third centrifugal force.

[0073] In one embodiment of the present invention, the plurality of washing chambers include a first washing chamber 1031, a second washing chamber 1032 and a third washing chamber 1033, a first connecting channel 110 is provided between the first washing chamber 1031 and the second washing chamber 1032, a first paraffin valve 111 is provided on the first connecting channel 110, a second connecting channel 112 is provided between the second washing chamber 1032 and the third washing chamber 1033, a second paraffin valve 113 is provided on the second connecting channel 112, the microfluidic chip also includes a second heating element and a third heating element respectively arranged below the first connecting channel 110 and the second connecting channel 112, the third rotation operation process includes a fourth rotation stage, a fifth rotation stage and a sixth rotation stage, step S203: control the driving element to drive the substrate 1 to perform the third rotation operation in the first centrifugal operation, so that the washing liquid in each washing chamber is thrown into the mixing chamber 105 in batches under the action of the third centrifugal force, and includes steps S301-step S305, wherein:

[0074] Step S301 : controlling the driving member to drive the base body 1 to perform a rotation operation corresponding to the fourth rotation stage, and the washing liquid in the first washing chamber 1031 is thrown into the mixing chamber 105 .

[0075] Specifically, in the radial direction of the substrate 1, the first washing chamber 1031, the second washing chamber 1032 and the third washing chamber 1033 are all on the inner side of the mixing chamber 105, and a sixth capillary channel 123 is provided between the first washing chamber 1031 and the mixing chamber 105. The sixth capillary channel 123 is a capillary valve, and the first siphon valve 109 is provided on the sixth capillary channel 123; the end of the second connecting channel 112 converges to the first connecting channel 110, and the end of the first connecting channel 110 converges to the sixth capillary channel 123, and the confluence of the first connecting channel 110 and the sixth capillary channel 123 is located at the upstream end of the first siphon valve 109. When the driving member drives the base 1 to perform the rotation operation corresponding to the fourth rotation stage, the third centrifugal force generated by the washing liquid in the first washing chamber 1031 enables it to enter the mixing chamber 105 through the first siphon valve 109. Due to the setting of the first paraffin valve 111 and the second paraffin valve 113, the first connecting channel 110 and the second connecting channel 112 are in a cut-off state, and the washing liquids in the second washing chamber 1032 and the third washing chamber 1033 cannot flow into the mixing chamber 105. The washing liquid in the first washing chamber 1031 performs the first washing of the nucleic acid. In this embodiment, the washing liquid in the first washing chamber 1031 is preferably buffer solution GD.

[0076] Step S302: controlling the second heating element to heat up so as to conduct the first connecting channel 110;

[0077] Step S303 : controlling the driving member to drive the base body 1 to perform a rotation operation corresponding to the fifth rotation stage, and the washing liquid in the second washing chamber 1032 is thrown into the mixing chamber 105 .

[0078] In order to wash the nucleic acid for the second time, the controller controls the second heating element to heat. After the second heating element is heated, the first paraffin valve 111 melts and the first connecting channel 110 is opened. When the driving element drives the substrate 1 to perform the rotation operation corresponding to the fifth rotation stage, the third centrifugal force generated by the washing liquid in the second washing chamber 1032 causes it to flow into the first connecting channel 110 and the sixth capillary channel 123 in sequence, and then enters the mixing chamber 105 through the first siphon valve 109. After the washing liquid in the second washing chamber 1032 enters the mixing chamber 105, the nucleic acid is washed for the second time. In this embodiment, the washing liquid in the second washing chamber 1032 is preferably the rinsing liquid PW1.

[0079] Step S304: controlling the third heating element to heat up so as to conduct the second connecting channel 112;

[0080] Step S305 : controlling the driving member to drive the base 1 to perform a rotation operation corresponding to the sixth rotation stage, and the washing liquid in the third washing chamber 1033 is thrown into the mixing chamber 105 .

[0081] In order to wash the nucleic acid for the third time, the controller controls the third heating element to heat. After the third heating element is heated, the second paraffin valve 113 melts and the second connecting channel 112 is opened; when the driving element drives the substrate 1 to perform the rotation operation corresponding to the sixth rotation stage, the third centrifugal force generated by the washing liquid in the third washing chamber 1033 causes it to flow into the second connecting channel 112, the first connecting channel 110 and the sixth capillary channel 123 in sequence, and then enters the mixing chamber 105 through the first siphon valve 109. After the washing liquid in the third washing chamber 1033 enters the mixing chamber 105, the nucleic acid is washed for the third time. In this embodiment, the washing liquid in the third washing chamber 1033 is preferably the rinsing liquid PW2, and the rinsing liquid PW1 and the rinsing liquid PW2 are both rinsing liquid PW, but the composition and / or proportion of each component are different.

[0082] Furthermore, in the present embodiment, the washing module 103 further includes a fourth washing chamber 124 disposed between the third washing chamber 1033 and the elution chamber 104, a fourth connecting channel 125 is disposed between the fourth washing chamber 124 and the third washing chamber 1033, a fourth paraffin valve 126 is disposed on the fourth connecting channel 125, and a sixth heating element is disposed below the fourth connecting channel 125; as the sample type is different, the type of washing solution pre-stored in the fourth washing chamber 124 is also different. Before nucleic acid testing, the testing personnel can select a microfluidic chip pre-stored with a corresponding type of washing solution in the fourth washing chamber 124 for nucleic acid testing according to the type of the sample. Acid detection, after the third washing is completed and the fourth washing is required, the controller controls the sixth heating element to heat, the fourth paraffin valve 126 to melt, and the fourth connecting channel 125 to be turned on. Then the controller controls the driving element to drive the substrate 1 to perform a corresponding rotation operation. The third centrifugal force generated by the washing liquid in the fourth washing chamber 124 causes it to flow into the fourth connecting channel 125, the second connecting channel 112, the first connecting channel 110 and the sixth capillary channel 123 in sequence, and then enters the mixing chamber 105 through the first siphon valve 109. After the washing liquid in the fourth washing chamber 124 enters the mixing chamber 105, the nucleic acid is washed for the fourth time.

[0083] In one embodiment of the present invention, the first centrifugation operation further includes a lysis waste liquid discharge operation and a washing waste liquid discharge operation, and step S101 of throwing the waste liquid generated in batches in the mixing chamber 105 into the waste liquid chamber 107 further includes steps S401-step S402, wherein:

[0084] Step S401 : after the third rotation stage is completed, the driving member is controlled to drive the base body 1 to perform a lysis waste liquid discharge operation, so as to eject the waste liquid generated by sample lysis in the mixing chamber 105 into the waste liquid chamber 107 .

[0085] Specifically, the storage chamber 106 and the mixing chamber 105 are on the same radial annular belt of the substrate 1. In the radial direction of the substrate 1, the waste liquid chamber 107 is located outside the mixing chamber 105 and the storage chamber 106. A second siphon valve 127 is provided between the waste liquid chamber 107 and the storage chamber 106. A column valve 128 is provided between the storage chamber 106 and the mixing chamber 105. A filter membrane (the filter membrane is made of silica gel) capable of adsorbing nucleic acids is provided on the column valve 128. Since impurities are generated during the sample lysis process, the anhydrous ethanol in the third liquid storage chamber 1023 enters the mixing chamber 105 to clean the nucleic acids and dissolve the impurities in the anhydrous ethanol. In order to avoid affecting the subsequent test results, the above-mentioned waste liquid should be discharged. Therefore, step S401 in this embodiment is performed after the anhydrous ethanol is thrown into the mixing chamber 105 and the controller controls the substrate 1 to be repeatedly centrifuged for a second preset time.

[0086] In step S401, the waste liquid generated by sample lysis in the mixing chamber 105 first enters the storage chamber 106 through the column valve 128, and then enters the waste liquid chamber 107 through the second siphon valve 127 by low-speed centrifugation. The lysed nucleic acid remains in the mixing chamber 105 due to the obstruction of the filter membrane. The nucleic acid can be washed only after the waste liquid is discharged, that is, step S203 is executed after step S401 is completed.

[0087] Step S402 : during the third rotation operation, the driving member is controlled to drive the base 1 to perform the washing waste liquid discharge operation multiple times at intervals, so as to throw the waste liquid generated in batches due to washing of the target substance in the mixing chamber 105 into the waste liquid chamber 107 .

[0088] Specifically, after step S301 is completed, a first washing waste liquid is generated in the mixing chamber 105 due to the first washing. In order to avoid affecting the subsequent washing effect, the first washing waste liquid should be discharged before the second washing, that is, the driving member drives the matrix 1 to perform the washing waste liquid discharge operation for the first time, so that the first washing waste liquid in the mixing chamber 105 first passes through the column valve 128 into the storage chamber 106, and then enters the waste liquid chamber 107 through the second siphon valve 127 through low-speed centrifugation. The nucleic acid after the first washing remains in the mixing chamber 105 due to the obstruction of the filter membrane; similarly, after steps S303 and S305 are completed respectively (if there is a fourth washing, then after the fourth washing is completed), the controller also controls the driving member to drive the matrix 1 to perform the washing waste liquid discharge operation, so that the waste liquid generated in the mixing chamber 105 is discharged before the elution operation.

[0089] In one embodiment of the present invention, the controller is further configured to perform step S501:

[0090] Step S501: After the first centrifugation operation is completed and before the second centrifugation operation, the first heating element is controlled to heat the mixing chamber 105 to evaporate the residual waste liquid in the mixing chamber 105 .

[0091] Step S501 is executed after step S402 is completed to avoid residual waste liquid in the mixing chamber 105 after step S402 is completed. To avoid the residual waste liquid from adversely affecting the subsequent elution operation, the controller controls the first heating element to heat the mixing chamber 105. The temperature in the mixing chamber 105 increases, and the residual waste liquid in the mixing chamber 105 evaporates, which is beneficial to improving the accuracy of nucleic acid detection.

[0092] Step S102 : controlling the driving member to drive the base body 1 to perform a second centrifugation operation, so as to successively throw the eluent into the receiving chamber 106 and the mixing chamber 105 .

[0093] Specifically, a seventh capillary channel 129 forming a capillary valve is provided between the inlet ends of the elution chamber 104 and the storage chamber 106, a fifth paraffin valve 130 is provided on the seventh capillary channel 129, and a seventh heating element is provided below the fifth paraffin valve 130. After step S501 is completed, the controller controls the seventh heating element to heat, the fifth paraffin valve 130 melts, and the seventh capillary channel 129 is turned on, and then the controller executes step S102, the centrifugal force generated by the eluent in the elution chamber 104 during the second centrifugation operation is greater than the capillary force in the seventh capillary channel 129, the eluent enters the storage chamber 106 through the seventh capillary channel 129, and then enters the mixing chamber 105 from the storage chamber 106 through the column valve 128 to elute the nucleic acid, wherein the eluent is preferably 50ul-200ul elution buffer TE. Furthermore, since some nucleic acids may be adsorbed on the filter membrane due to inertia during the process of discarding the waste liquid, when the elution liquid flows into the mixing chamber 105 from the direction of the storage chamber 106, the sample nucleic acid adsorbed on the filter membrane can be washed, and the adsorbed nucleic acid can be returned to the mixing chamber 105 for elution, so that all nucleic acids can experience the elution stage more completely and fully.

[0094] In one embodiment of the present invention, a third connecting channel 114 is provided between the mixing chamber 105 and the amplification module 108, a third paraffin valve 115 is provided on the third connecting channel 114, and a fourth heating element is provided below the third paraffin valve 115. The controller is further configured to perform steps S601-S602, wherein:

[0095] Step S601: after the eluent is thrown into the mixing chamber 105, the fourth heating element is controlled to heat to connect the third connecting channel 114;

[0096] Step S602: after the third connecting channel 114 is opened, the driving member is controlled to drive the base body 1 to perform a third centrifugal operation.

[0097] Specifically, after the eluent enters the mixing chamber 105, the rotation of the matrix 1 is stopped first, and the eluent is allowed to stand in the mixing chamber 105 for 2 minutes to 5 minutes. Then, the rotation of the matrix 1 is controlled so that the eluent and the nucleic acid are fully mixed for elution. After the elution is completed, the controller controls the fourth heating element to heat, the third paraffin valve 115 to melt, and the third connecting channel 114 to be turned on; after the third connecting channel 114 is turned on, the controller controls the driving element to drive the matrix 1 to perform the third centrifugation operation, so that the mixed liquid of the eluent and the nucleic acid (that is, the treated detection liquid in this embodiment refers to the mixed liquid of the eluent and the nucleic acid) enters the amplification module 108 through the third connecting channel 114.

[0098] Step S103: controlling the driving member to drive the base body 1 to perform a third centrifugation operation, so as to throw the processed detection liquid into the amplification module 108 to mix with the solid reagent and generate an amplification reaction liquid.

[0099] In one embodiment of the present invention, the amplification module 108 includes a detection chamber 1081 in which a solid reagent is pre-stored (the solid reagent in this embodiment may be a freeze-dried detection reagent ball) and an amplification chamber 1082 located at the downstream end of the detection chamber 1081. The substrate 1 is further provided with a paraffin chamber 116 and a fifth heating element located below the paraffin chamber 116. The paraffin chamber 116 is connected to the third connecting channel 114. The controller is further configured as steps S701-S702, wherein:

[0100] Step S701 : after the third centrifugation operation is completed, the driving member is controlled to drive the base 1 to perform a fourth centrifugation operation, so that the amplification reaction liquid in the detection chamber 1081 is thrown into the amplification chamber 1082 .

[0101] Specifically, in this embodiment, there are multiple detection cavities 1081, and the multiple detection cavities 1081 are evenly distributed along the axial direction of the substrate 1. There are multiple amplification cavities 1082, and the multiple amplification cavities 1082 correspond to the multiple detection cavities 1081 one by one and are connected by a capillary valve. Different types of solid reagents can be pre-stored in each detection cavity 1081, and the treated detection liquid is mixed with different types of solid reagents to form different types of amplification reaction liquid and can flow into the corresponding amplification cavity 1082, so that the detection module can detect multiple targets in the nucleic acid at the same time.

[0102] Furthermore, during the third centrifugation operation, the treated detection liquid first enters the detection chamber 1081 of the amplification module 108 through the third connecting channel 114, and is mixed with the solid reagent to form an amplification reaction liquid; then the controller executes step S701 to allow the amplification reaction liquid to enter the amplification chamber 1082 through the capillary valve between the detection chamber 1081 and the corresponding amplification chamber 1082, and the detection module can perform fluorescence detection on the amplification reaction liquid in the amplification chamber 1082.

[0103] Step S702 : After the fourth centrifugation operation is completed, the fifth heating element is controlled to heat so that the paraffin pre-stored in the paraffin chamber 116 is melted and the amplification module 108 is sealed.

[0104] In order to prevent the amplification reaction liquid in the amplification chamber 1082 from flowing back due to other factors before the detection module performs detection, thereby causing insufficient capacity of the amplification reaction liquid in the amplification chamber 1082 and reducing the accuracy of the detection result, the amplification module 108 needs to be closed, that is, the controller controls the fifth heating element to heat, and the paraffin pre-stored in the paraffin chamber 116 melts and flows into the third connecting channel 114, and then continues to flow along the third connecting channel 114 toward each detection chamber 1081 in the sitting direction until the entrance end of each detection chamber 1081 is completely blocked. After the paraffin is cooled and solidified, it can block each detection chamber 1081. When each detection chamber 1081 is blocked, each amplification chamber 1082 is also blocked, and the amplification reaction liquid can no longer flow out of the amplification module 108.

[0105] Step S104: controlling the detection module to detect the amplification reaction solution.

[0106] Specifically, after the detection module is started, the nucleic acid detection process is completed through thermal cycling, lighting, and detection.

[0107] Another embodiment of the present invention provides a novel detection device, which includes the microfluidic chip for the detection device in the above embodiment, a driving member for driving the above microfluidic chip to rotate, and a detection module.

[0108] The present invention provides a microfluidic chip for a detection device and the detection device. The detection device comprises a microfluidic chip, a driving member for driving the microfluidic chip to rotate and a detection module. The microfluidic chip comprises a substrate and a controller. The substrate is provided with a sample cavity for accommodating a sample, a liquid storage module, a washing module, an elution cavity, a mixing cavity, a storage cavity, a waste liquid cavity and an amplification module. The controller is communicatively connected with the driving member and the detection module and configured to: control the driving member to drive the substrate to perform a first centrifugal operation to throw the sample, the reagent and the washing liquid into the mixing cavity in batches, and throw the waste liquid generated in the mixing cavity in batches into the waste liquid cavity; control the driving member to drive the substrate to perform a second centrifugal operation to throw the elution liquid into the storage cavity and the mixing cavity in turn; control the driving member to drive the substrate to perform a third centrifugal operation to throw the treated detection liquid into the amplification module to mix with the solid reagent and generate an amplification reaction liquid; and control the detection module to detect the amplification reaction liquid. The microfluidic chip has a simple structure and a reasonable layout, which realizes the miniaturization and lightweight design of the microfluidic chip, distinguishes the independent detection of multiple targets in the target substance, ensures that the properties of the processed detection liquid and solid reagent entering each detection chamber are consistent, avoids the cross-mixing problem of each detection chamber, and is beneficial to improving the detection accuracy; when the detection personnel use the above-mentioned microfluidic chip for nucleic acid detection, they only need to inject the sample into the sample chamber and then install the microfluidic chip. The controller drives the matrix to automatically perform the centrifugation operation and detect the target substance in the sample. The operation is simple and reduces the labor intensity of the detection personnel, which is beneficial to improving the detection efficiency and ensuring the stability of the detection results.

[0109] The preferred embodiments of the present invention are described in detail above in conjunction with the accompanying drawings, but the present invention is not limited thereto. Within the technical concept of the present invention, the technical solution of the present invention can be subjected to a variety of simple modifications, including the combination of various specific technical features in any appropriate manner. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations. However, these simple modifications and combinations should also be regarded as the contents disclosed by the present invention and belong to the protection scope of the present invention.

[0110] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral one; it can be a mechanical connection, an electrical connection, or communication with each other; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0111] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0112] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations of the present invention. A person skilled in the art may change, modify, replace and vary the above embodiments within the scope of the present invention.

Claims

1. A microfluidic chip for a detection device, the detection device comprising a microfluidic chip, a driving member for driving the microfluidic chip to rotate, and a detection module, characterized in that: The microfluidic chip comprises: A substrate (1), wherein the substrate (1) is provided with a sample chamber (101) for accommodating a sample, a liquid storage module (102), a washing module (103), an elution chamber (104), a mixing chamber (105), a storage chamber (106), a waste liquid chamber (107) and an amplification module (108), wherein the liquid storage module (102) is pre-stored with a reagent for lysing the sample, the washing module (103) is pre-stored with a washing liquid, the elution chamber (104) is pre-stored with an elution liquid, the amplification module (108) is pre-stored with a solid reagent, and the sample chamber (101) is pre-stored with a liquid storage module (102). 101), the liquid storage module (102) and the washing module (103) are all connected to the inlet end of the mixing chamber (105), the waste liquid chamber (107) is located at the downstream end of the mixing chamber (105), the storage chamber (106) is located between the mixing chamber (105) and the waste liquid chamber (107) and connects the mixing chamber (105) and the waste liquid chamber (107), the elution chamber (104) is connected to the inlet end of the storage chamber (106), and the amplification module (108) is connected to the outlet end of the mixing chamber (105); A controller is communicatively connected with the driving member and the detection module and is configured as follows: Controlling the driving member to drive the base (1) to perform a first centrifugal operation, so as to throw the sample, the reagent and the washing liquid into the mixing chamber (105) in batches, and throw the waste liquid generated in batches in the mixing chamber (105) into the waste liquid chamber (107); Controlling the driving member to drive the base (1) to perform a second centrifugation operation, so as to successively throw the eluent into the receiving chamber (106) and the mixing chamber (105); Controlling the driving member to drive the base (1) to perform a third centrifugation operation, so as to throw the treated detection liquid into the amplification module (108) to mix with the solid reagent and generate an amplification reaction liquid; The detection module is controlled to detect the amplification reaction solution.

2. The microfluidic chip for detection equipment according to claim 1, characterized in that: The sample chamber (101), the liquid storage module (102) and the washing module (103) are distributed on the substrate (1) at intervals along the circumference of the substrate (1); capillary valves are provided between the sample chamber (101), the liquid storage module (102) and the mixing chamber (105); a first siphon valve (109) is provided between the washing module (103) and the mixing chamber (105); the liquid storage module (102) comprises a plurality of liquid storage chambers distributed at intervals along the radial direction of the substrate (1); the washing module (103) comprises a plurality of washing chambers distributed at intervals along the circumference of the substrate (1); and controlling the driving member to drive the substrate (1) to perform a first centrifugal operation so as to throw the sample, the reagent and the washing solution into the mixing chamber (105) in batches comprises: Controlling the driving member to drive the base (1) to perform a first rotation operation in the first centrifugal operation, so that the sample is thrown into the mixing chamber (105) under the action of a first centrifugal force; Controlling the driving member to drive the base (1) to perform a second rotation operation in the first centrifugal operation, so that the reagents in each of the liquid storage chambers are thrown into the mixing chamber (105) in batches under the action of the second centrifugal force; The driving member is controlled to drive the base (1) to perform a third rotation operation in the first centrifugal operation, so that the washing liquid in each washing chamber is thrown into the mixing chamber (105) in batches under the action of the third centrifugal force.

3. The microfluidic chip for detection equipment according to claim 2, characterized in that: The plurality of liquid storage chambers include a first liquid storage chamber (1021), a second liquid storage chamber (1022) and a third liquid storage chamber (1023), and the controlling the driving member to drive the base (1) to perform the second rotation operation in the first centrifugal operation so that the reagents in each of the liquid storage chambers are thrown into the mixing chamber (105) in batches under the action of the second centrifugal force includes: The driving member is controlled to drive the base (1) to perform the second rotation operation, wherein the second rotation operation process includes a first rotation stage, a second rotation stage and a third rotation stage in which the second centrifugal forces are different, the reagent in the first liquid storage chamber (1021) is thrown into the mixing chamber (105) in the first rotation stage, the reagent in the second liquid storage chamber (1022) is thrown into the mixing chamber (105) in the second rotation stage, and the reagent in the third liquid storage chamber (1023) is thrown into the mixing chamber (105) in the third rotation stage.

4. The microfluidic chip for detection equipment according to claim 3, characterized in that: The microfluidic chip further comprises a first heating element disposed below the mixing chamber (105) and in communication with the controller, and the controller is further configured to: The first heating element is controlled to heat the mixing chamber (105) at a preset temperature for a first preset time period between the second rotation stage and the third rotation stage.

5. The microfluidic chip for detection equipment according to claim 4, characterized in that: The plurality of washing chambers include a first washing chamber (1031), a second washing chamber (1032) and a third washing chamber (1033); a first connecting channel (110) is provided between the first washing chamber (1031) and the second washing chamber (1032); a first paraffin valve (111) is provided on the first connecting channel (110); a second connecting channel (112) is provided between the second washing chamber (1032) and the third washing chamber (1033); a second paraffin valve (113) is provided on the second connecting channel (112); the microfluidic chip further includes a second heating element and a third heating element respectively arranged below the first connecting channel (110) and the second connecting channel (112); the third rotation operation process includes a fourth rotation stage, a fifth rotation stage and a sixth rotation stage; the controlling the driving element to drive the substrate (1) to perform the third rotation operation in the first centrifugal operation so that the washing liquid in each of the washing chambers is thrown into the mixing chamber (105) in batches under the action of the third centrifugal force includes: Controlling the driving member to drive the base body (1) to perform a rotation operation corresponding to the fourth rotation stage, so that the washing liquid in the first washing chamber (1031) is thrown into the mixing chamber (105); controlling the second heating element to heat so as to open the first connecting channel (110); Controlling the driving member to drive the base body (1) to perform a rotation operation corresponding to the fifth rotation stage, so that the washing liquid in the second washing chamber (1032) is thrown into the mixing chamber (105); Controlling the third heating element to heat so as to open the second connecting channel (112); The driving member is controlled to drive the base body (1) to perform a rotation operation corresponding to the sixth rotation stage, and the washing liquid in the third washing chamber (1033) is thrown into the mixing chamber (105).

6. The microfluidic chip for detection equipment according to claim 5, characterized in that: The first centrifugation operation further includes a lysis waste liquid discharge operation and a washing waste liquid discharge operation, wherein the waste liquid generated in batches in the mixing chamber (105) is thrown into the waste liquid chamber (107) including: After the third rotation stage is completed, the driving member is controlled to drive the base body (1) to perform the lysis waste liquid discharge operation, so as to eject the waste liquid generated by sample lysis in the mixing chamber (105) into the waste liquid chamber (107); During the third rotation operation, the driving member is controlled to drive the base (1) to perform the washing waste liquid discharge operation at intervals for multiple times, so as to eject the waste liquid generated in batches due to washing of the target substance in the mixing chamber (105) into the waste liquid chamber (107).

7. The microfluidic chip for detection equipment according to claim 6, characterized in that: The controller is also configured to: After the first centrifugation operation is completed and before the second centrifugation operation, the first heating element is controlled to heat the mixing chamber (105) so as to evaporate the residual waste liquid in the mixing chamber (105).

8. The microfluidic chip for detection equipment according to claim 6, characterized in that: A third connecting channel (113) is provided between the mixing chamber (105) and the amplification module (108), a third paraffin valve (115) is provided on the third connecting channel (113), a fourth heating element is provided below the third paraffin valve (115), and the controller is further configured as follows: After the eluent is thrown into the mixing chamber (105), controlling the fourth heating element to heat so as to open the third connecting channel (113); After the third connecting channel (113) is opened, the driving member is controlled to drive the base body (1) to perform a third centrifugal operation.

9. The microfluidic chip for detection equipment according to claim 8, characterized in that: The amplification module (108) comprises a detection chamber (1081) in which the solid reagent is pre-stored and an amplification chamber (1082) located at the downstream end of the detection chamber (1081); the base (1) is further provided with a paraffin chamber (116) and a fifth heating element located below the paraffin chamber (116); the paraffin chamber (116) is connected to the third connection channel (113); and the controller is further configured as follows: After the third centrifugal operation is completed, controlling the driving member to drive the base body (1) to perform a fourth centrifugal operation, so that the amplification reaction liquid in the detection chamber (1081) is thrown into the amplification chamber (1082); After the fourth centrifugation operation is completed, the fifth heating element is controlled to heat so that the paraffin pre-stored in the paraffin chamber (116) melts and seals the amplification module (108).

10. A detection device, characterized in that: The detection device comprises a microfluidic chip for a detection device according to any one of claims 1 to 9, a driving member for driving the microfluidic chip to rotate, and a detection module.

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