Microorganism expanding culture device and expanding culture method thereof

Abstract

The invention relates to the field of microorganism culture devices, and discloses a microorganism expanding culture device and an expanding culture method thereof. The microorganism expanding culture device comprises a cabinet body, wherein an expanding culture reaction mechanism, a supply mechanism and a control mechanism are arranged in the cabinet body; the propagation reaction mechanism comprises a propagation reaction cylinder, a cylinder cover plate, an electric device and a stirring assembly; the propagation reaction cylinder is fixedly connected with the cabinet body; the cylinder cover plate is connected with the electric device; the power output end of the electric device is connected with the stirring assembly through the barrel cover plate; the stirring assembly is arranged in the propagation reaction cylinder; the supply mechanism is connected with the barrel cover plate and comprises a hot water supply assembly, and the hot water supply assembly is connected with the barrel cover plate. The temperature suitable for efficient propagation of strains can be achieved when the temperature is low in winter, the intelligent degree of the propagation process is improved, accurate monitoring of the propagation process and device integration of subsequent equipment cleaning are achieved, and efficient propagation of microorganisms is achieved on the whole.

Description

Technical Field

[0001] The present invention relates to the field of microorganism cultivation equipment, and more specifically, to a microorganism expansion cultivation device and an expansion cultivation method thereof. Background Art

[0002] Traditional pig feed mainly relies on grains, soybean meal, fish meal and other ingredients. Although it can provide basic nutritional needs, in actual application, it often faces problems such as nutritional imbalance, low digestion and absorption rate, feed pollution, and drug residues. These problems not only affect the growth and health of pigs, but may also lead to reduced economic benefits and food safety risks. Nutritional imbalance is one of the main problems of existing pig feed. The imbalance of protein, vitamins and minerals in the feed makes it impossible for pigs to obtain comprehensive nutrition, which in turn affects their growth rate and immunity; secondly, some feed ingredients such as high-fiber substances and protein sources containing anti-nutritional factors are difficult for pigs to digest and absorb, resulting in nutritional waste and low feed utilization; in addition, during the production, storage and transportation of feed, it is easy to be contaminated by mycotoxins, heavy metals, etc., which seriously threatens the health of pigs and the safety of products.

[0003] In order to increase growth rate, some farmers add antibiotics and growth promoters to feed. Although the effect is obvious in the short term, long-term use will lead to drug residues, affecting the quality of pork and food safety. At the same time, the cost of high-quality feed is high, making it difficult for some small-scale farmers to afford it. Choosing low-cost and low-quality feed will further aggravate the above problems.

[0004] In order to solve these problems, recent studies have found that adding appropriate bacterial liquid to pig feed is an innovative method to effectively improve the health level and growth performance of pigs, which has broad application prospects and significant economic benefits. The beneficial microorganisms in the bacterial liquid can optimize the intestinal microbial environment of pigs, enhance their digestion and absorption functions, improve feed utilization, and enhance pig immunity and reduce the incidence of diseases by competitively inhibiting pathogens; in addition, the bacterial liquid can also decompose anti-nutritional factors in feed and release more nutrients for pigs to use.

[0005] If the number of pigs raised by pig farmers is too large, they need to consider adding bacterial liquid that is beneficial to pig growth and immunity to the feed on a daily basis, which is a huge expense for pig farmers. Therefore, it is possible to allow farmers to independently expand the strains that need to be added to pig feed, so that it becomes a part of the pig breeding process.

[0006] Patents on microbial expansion devices in the prior art, such as the patent with announcement number CN 220413392 U, disclose a microbial expansion device, including a reaction mechanism and a feeding mechanism that are separately arranged. During operation, the water pump and the feed pump automatically replenish the reaction container with reaction water and nutrient solution according to the feedback of the detection sensor, thereby achieving continuous and efficient microbial expansion. However, the device cannot meet the control of the required temperature for bacterial expansion, and there is no corresponding temperature monitoring device to monitor the temperature of the bacterial solution during the expansion process; in addition, the device does not take into account the cleaning and disinfection of the equipment after the bacterial solution expansion. Summary of the invention

[0007] The present invention provides a microbial culture device to overcome the defects of the prior art, such as the lack of control of the required temperature for culture expansion, the lack of temperature monitoring during the culture expansion, and the lack of cleaning and disinfection of the equipment after the culture expansion.

[0008] Another object of the present invention is to provide a culture expansion method using the above device.

[0009] In order to solve the above technical problems, the technical solution of the present invention is as follows:

[0010] A microorganism expansion and cultivation device comprises a cabinet, wherein an expansion and cultivation reaction mechanism and a supply mechanism are arranged in the cabinet;

[0011] The expansion reaction mechanism comprises an expansion reaction cylinder and a cylinder cover plate for sealing the expansion reaction cylinder; the expansion reaction cylinder is fixedly connected to the cabinet;

[0012] The expansion reaction mechanism also includes an electric device and a stirring assembly; the cylinder cover is connected to the electric device; the power output end of the electric device is connected to the stirring assembly through the cylinder cover; the stirring assembly is arranged inside the expansion reaction cylinder;

[0013] The supply mechanism is connected to the cylinder cover plate and is used to add the raw materials required for the expansion reaction cylinder for expansion; the supply mechanism includes a hot water supply component, and the hot water supply component is connected to the cylinder cover plate;

[0014] The microorganism expansion and cultivation device is also provided with a control mechanism for monitoring and regulating the expansion and cultivation reaction mechanism and the supply mechanism; the control mechanism is electrically connected to the expansion and cultivation reaction mechanism and the supply mechanism respectively.

[0015] Furthermore, the supply mechanism further comprises a feeding window; the cylinder cover is provided with a feeding port connected to the feeding window;

[0016] The hot water supply assembly includes a water heater and a delivery pipeline for delivering water required for the reaction; the pipe body of the delivery pipeline is wrapped with a layer of polyurethane foam insulation sleeve; the delivery pipeline heats the water in the water heater, and then transmits the heated water required for the expansion to the expansion reaction cylinder through the delivery pipeline;

[0017] The heater storage cabinet is provided at the obliquely lower side of the cabinet layer where the expansion culture reaction cylinder is located, and the water heater is fixedly installed on the inner wall of the heater storage cabinet;

[0018] The cylinder cover is also provided with a negative pressure regulating valve for regulating the pressure of the internal expansion process according to the type of cultured bacteria; the negative pressure regulating valve is arranged around the circumference of the panel of the cylinder cover;

[0019] The cylinder cover is provided with a sealing strip groove, in which a sealing ring strip is installed, and the expansion reaction cylinder is sealed and connected with the cylinder cover through the sealing strip groove on the cylinder cover.

[0020] Further, the control mechanism includes a controller, an indicator light and a sensor;

[0021] The indicator light and the sensor are electrically connected to the controller;

[0022] The sensors include a temperature sensor, a pH sensor, a dissolved oxygen sensor and a pressure sensor arranged in the expansion and cultivation reaction cylinder, and also include a humidity sensor arranged on the inner wall of the cabinet of the microorganism expansion and cultivation device;

[0023] The sensor is used to monitor and determine the changes in temperature parameters, pH parameters, dissolved oxygen parameters and pressure parameters during the bacterial liquid expansion process inside the expansion reaction cylinder; the humidity sensor is used to monitor and determine the changes in the gas humidity inside the cabinet.

[0024] Furthermore, the bottom of the expansion and cultivation reaction cylinder is set to be conical; the conical transition part of the cylinder of the expansion and cultivation reaction cylinder is clamped and fixed on the layered rack built into the cabinet;

[0025] The conical bottom of the expansion culture reaction cylinder is provided with a bacterial liquid outlet; the reaction mechanism also includes a liquid outlet faucet, and the bacterial liquid outlet is connected to the liquid outlet faucet through a conveying pipeline; a bacterial liquid barrel for loading bacterial liquid is placed below the outlet of the liquid outlet faucet; the microbial expansion culture device is also provided with a bacterial liquid storage cabinet for placing the bacterial liquid barrel; the bacterial liquid storage cabinet is arranged on the other side of the cabinet layer obliquely below the expansion culture reaction cylinder.

[0026] Furthermore, a rotatable high-pressure washing nozzle and a water supply pipe port connected to the high-pressure washing nozzle are provided on the circumference of the cylinder wall of the expansion reaction cylinder, and the water supply pipe port is connected to the water inlet of the high-pressure washing nozzle; a threaded ball with a controllable rotation angle is provided at the rotating head of the high-pressure washing nozzle, and a nozzle is provided at the end of the nozzle;

[0027] The hot water supply component transmits hot water to the high-pressure washing nozzle through the water supply pipe port; it is used to clean the inside of the expansion reaction cylinder after the bacterial culture expansion is completed;

[0028] A waterproof camera for monitoring the stirring and cleaning conditions inside the cylinder is provided on the inner side panel of the cylinder cover, and the waterproof camera is electrically connected to the controller.

[0029] The expansion culture reaction cylinder also includes an ultraviolet sterilization light strip; the expansion culture reaction cylinder has sunken grooves evenly distributed on the circumference of the cylinder wall, and the ultraviolet sterilization light strip is embedded in the sunken grooves. The ultraviolet sterilization light strip is electrically connected to the controller and is used to remove residual bacteria after cleaning the inside of the expansion culture reaction cylinder.

[0030] Furthermore, drying fans are provided on both sides of the inner wall of the cabinet of the microorganism cultivation device to adjust the gas humidity inside the cabinet of the cultivation reaction cylinder during the cultivation process;

[0031] A layer of graphene heating film is laid on the bottom plate of the layered rack; the graphene heating film is electrically connected to the control mechanism, and the drying fan is also used to accelerate the circulation of the heated air flow in the cabinet;

[0032] The inner wall of the cabinet is also provided with a heating device for maintaining a constant temperature on the expansion reaction cylinder, and the heating device is electrically connected to the control mechanism; the inner wall of the cabinet is also provided with heat-insulating cotton for reducing heat dissipation and reducing energy consumption of the device.

[0033] Furthermore, the supply mechanism also includes a pressurized water pump. A hot water delivery port is provided on the cylinder cover. After the hot water is heated by the water heater, the pressurized water pump delivers the reaction hot water used for microbial expansion through a delivery pipeline from the hot water delivery port to the inside of the expansion reaction cylinder.

[0034] Furthermore, two water heaters are provided, and a series two-stage heating system is used to first heat a portion of the reaction water through the first water heater, and then the water heated by the first water heater is heated to 40°C-42°C through the second water heater.

[0035] Furthermore, a water flow sensor is provided in the water heater to monitor and measure the heating process and the flow of hot water injected into the expansion reaction cylinder.

[0036] The present invention provides a microbial expansion method implemented by using the above microbial expansion device, comprising the following steps:

[0037] S1: The staff first confirms the cleanliness and sterility of the inside and outside of the expansion device, and checks the control mechanism and waterproof camera to ensure that the equipment is in normal working condition; at the same time, confirm the material input ratio;

[0038] S2: adding materials, putting the strains and culture materials required for expansion culture into the expansion culture reaction cylinder through the supply mechanism;

[0039] S3: the control mechanism starts the supply mechanism to inject the water required for the expansion into the expansion reaction cylinder through the cylinder cover;

[0040] S4: the control mechanism starts the electric device and the stirring assembly to work;

[0041] S5: The staff monitors and controls the temperature, pH, dissolved oxygen, pressure, and humidity in the expansion reaction cylinder during the expansion process through the waterproof camera and the control mechanism;

[0042] S6: After the microbial expansion device is completed, bacterial liquid is obtained. The staff cleans and disinfects the device through the control mechanism. After the high-pressure washing nozzle equipment is cleaned, the cleaning waste water is discharged through the liquid outlet faucet; finally, the ultraviolet sterilization light belt is used to disinfect the entire equipment of the expansion reaction cylinder.

[0043] Compared with the prior art, the technical solution of the present invention has the following beneficial effects:

[0044] 1. The present invention adopts the use of an electric device and a stirring assembly to accelerate the uniform mixing and reaction of the bacterial solution and improve the expansion efficiency; a pressurized water pump and a series-type secondary heating system are used to provide a constant and suitable expansion environment for the expansion of the strains. Even if the strains need to be expanded in winter, the temperature of the reaction water is low and the temperature suitable for the efficient expansion of the strains can be reached, thereby achieving efficient expansion of microorganisms as a whole.

[0045] 2. The present invention combines a control mechanism with a sensor and a controller to monitor key parameters such as temperature, pH, dissolved oxygen and pressure of the bacterial liquid during the expansion process in real time, ensuring that the expansion process is carried out under the best conditions, improving the expansion effect and efficiency, automating the entire process, reducing manual intervention, improving the intelligence of the expansion process, and realizing accurate monitoring of the expansion process.

[0046] 3. The present invention integrates the expansion process and the subsequent equipment cleaning device by adopting a high-pressure flushing nozzle, an ultraviolet sterilization lamp, and a supply mechanism and an expansion reaction mechanism, thereby simplifying the equipment cleaning process as a whole, ensuring equipment hygiene, and reducing the risk of contamination. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] Figure 1 This is a schematic diagram of the structure of a microorganism expansion and cultivation device according to an embodiment of the present invention;

[0048] Figure 2 This is a schematic diagram of the structure of a microorganism expansion and cultivation device according to an embodiment of the present invention;

[0049] Figure 3 It is a schematic cross-sectional view of a culture expansion reaction cylinder, a cylinder cover plate, an electric device and a stirring assembly according to an embodiment of the present invention;

[0050] Figure 4 This is a schematic diagram of the structure of the expansion reaction cylinder, cylinder cover, electric device and stirring assembly according to an embodiment of the present invention;

[0051] Figure 5 This is a schematic diagram of the structure of the cylinder cover plate according to an embodiment of the present invention;

[0052] Among them: 1. expansion reaction mechanism; 101. expansion reaction cylinder; 102. cylinder cover; 103. electric device; 104. stirring assembly; 105. liquid outlet faucet; 1011. bacterial liquid outlet; 1012. high-pressure washing nozzle; 1021. hot water delivery port; 1022. feeding port;

[0053] 2. Supply mechanism; 201. Hot water supply assembly; 202. Feeding window; 2011. Water heater;

[0054] 3. Control mechanism; 301. Controller; 302. Indicator light; 4. Drying fan. DETAILED DESCRIPTION

[0055] In order to make the purpose, technical scheme and advantages of the embodiments of the present application clearer, the technical scheme of the present application will be clearly and completely described below in conjunction with the drawings of the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all of the embodiments. Based on the described embodiments, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0056] Unless otherwise defined, the technical terms or scientific terms used in this application should be understood by people with ordinary skills in the field to which this application belongs. "First", "second" and similar words used in this application do not indicate any order, quantity or importance, but are only used to distinguish different components. "Include" or "comprise" and similar words mean that the elements or objects appearing before the word cover the elements or objects listed after the word and their equivalents, without excluding other elements or objects. "Connect" or "connected" and similar words are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "Up", "down", "left", "right" and the like are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0057] Example 1

[0058] like Figure 1-5 As described above, this embodiment discloses a microorganism expansion culture device and a microorganism expansion culture method.

[0059] A microorganism expansion and cultivation device comprises a cabinet, in which an expansion and cultivation reaction mechanism 1 and a supply mechanism 2 are arranged;

[0060] The expansion reaction mechanism 1 comprises an expansion reaction cylinder 101 and a cylinder cover plate 102 for sealing the expansion reaction cylinder 101; the expansion reaction cylinder 101 is fixedly connected to the cabinet;

[0061] The expansion reaction mechanism 1 also includes an electric device 103 and a stirring assembly 104; the cylinder cover 102 is connected to the electric device 103; the power output end of the electric device 103 is connected to the stirring assembly 104 through the cylinder cover 102; the stirring assembly 104 is arranged inside the expansion reaction cylinder 101;

[0062] The supply mechanism 2 is connected to the cylinder cover plate 102 and is used to add the raw materials required for the expansion reaction cylinder 101 for expansion. The supply mechanism 2 includes a hot water supply component 201, which is connected to the cylinder cover plate 102.

[0063] The microorganism expansion and cultivation device is also provided with a control mechanism 3 for monitoring and regulating the expansion and cultivation reaction mechanism 1 and the supply mechanism 2; the control mechanism 3 is electrically connected to the expansion and cultivation reaction mechanism 1 and the supply mechanism 2 respectively.

[0064] The supply mechanism 2 also includes a feeding window 202; the cylinder cover plate 102 is provided with a feeding port 1022 connected to the feeding window 202;

[0065] The hot water supply component 201 includes a water heater 2011 and a delivery pipeline for delivering water required for the reaction; the pipe body of the delivery pipeline is wrapped with a layer of polyurethane foam insulation sleeve; the delivery pipeline heats the incoming water through the water heater 2011, and then transmits the heated water required for expansion through the delivery pipeline to the expansion reaction cylinder 101; the polyurethane foam insulation sleeve can not only reduce heat loss, but also reduce the energy consumption of the water heater. The water temperature in the pipeline is better maintained, and the water heater does not need to be heated frequently, which greatly improves the energy utilization efficiency of the entire system.

[0066] A heater storage cabinet is provided at the obliquely lower side of the cabinet layer where the expansion culture reaction cylinder 101 is located, and a water heater (2011) is fixedly installed on the inner wall of the heater storage cabinet;

[0067] The cylinder cover 102 is also provided with a negative pressure regulating valve for regulating the pressure of the internal expansion culture process according to the type of cultured bacteria; the negative pressure regulating valve is arranged around the circumference of the panel of the cylinder cover 102; the cylinder cover 102 is provided with a sealing strip groove, and a sealing ring strip is installed in the sealing strip groove, and the expansion culture reaction cylinder 101 is sealed and connected to the cylinder cover 102 through the sealing strip groove on the cylinder cover 102.

[0068] The control mechanism 3 includes a controller 301, an indicator light 302 and a sensor;

[0069] The indicator light 302 and the sensor are electrically connected to the controller 301;

[0070] The sensors include a temperature sensor, a pH sensor, a dissolved oxygen sensor and a pressure sensor arranged in the expansion and cultivation reaction cylinder 101, and also include a humidity sensor arranged on the inner wall of the cabinet of the microorganism expansion and cultivation device;

[0071] The sensor is used to monitor and determine the changes in temperature parameters, pH parameters, dissolved oxygen parameters and pressure parameters during the bacterial liquid expansion process in the expansion reaction cylinder 101; the humidity sensor is used to monitor and determine the changes in the gas humidity in the cabinet.

[0072] The bottom of the expansion and cultivation reaction tube 101 is set to be conical; the conical transition of the expansion and cultivation reaction tube 101 is clamped and fixed on the layered rack built into the cabinet;

[0073] A bacterial liquid outlet 1011 is provided at the conical bottom of the expansion culture reaction cylinder 101; the reaction mechanism 1 also includes a liquid outlet faucet 105, and the bacterial liquid outlet 1011 is connected to the liquid outlet faucet 105 through a conveying pipe; a bacterial liquid barrel for loading bacterial liquid is placed below the outlet of the liquid outlet faucet 105; the microbial expansion culture device is also provided with a bacterial liquid storage cabinet for placing the bacterial liquid barrel; the bacterial liquid storage cabinet is arranged at the other side of the cabinet layer obliquely below the expansion culture reaction cylinder 101.

[0074] The microbial expansion method using the microbial expansion device of this embodiment comprises the following steps:

[0075] S1: The bacteria species and culture materials required for expansion and cultivation are put into the expansion and cultivation reaction cylinder 101 through the supply mechanism 2;

[0076] S2: The control mechanism 3 starts the supply mechanism 2 to inject the water required for the expansion into the expansion reaction cylinder 101 through the cylinder cover 102;

[0077] S3: The control mechanism 3 starts the electric device 103 and the stirring assembly 104 to work;

[0078] S4: The staff monitors and controls the temperature, pH, dissolved oxygen, pressure and humidity parameters in the expansion reaction cylinder 101 during the expansion process through the control mechanism 3;

[0079] S5: After the microbial expansion and cultivation device is completed, a bacterial liquid is obtained, and the staff cleans and disinfects the device.

[0080] More specific implementation steps are:

[0081] Before carrying out the actual microbial expansion preparation work, the staff first confirms the cleanliness and sterility of the inside and outside of the expansion device, and at the same time checks through the controller 301 that the device and all sensors are in normal working condition to ensure that the device is working properly;

[0082] When adding raw materials, the staff confirms in advance that the amount added meets the preset expansion formula, then opens the cylinder cover 102, and adds the microbial strains and culture materials (such as syrup, etc.) that need to be expanded into the expansion reaction cylinder 101 through the feeding window 202 and the feeding port 1022;

[0083] Then, water for the expansion reaction is added. At this time, the hot water supply component 201 is started to heat the required water to a suitable temperature (40° C.-42° C.); the heated expansion water is transported from the hot water delivery port 1021 to the expansion reaction cylinder 101 through the delivery pipeline; and the negative pressure regulating device is used to adjust the pressure inside the expansion reaction cylinder to ensure the best expansion environment;

[0084] Then, stirring is started. At this time, the staff starts the controller 301 of the control mechanism 3, and then turns on the electric device 103 and the stirring component 104 to stir, so as to ensure that the bacterial liquid and the culture material are fully mixed; at the same time, the controller 301 is used to set the expansion stirring time and stirring speed to ensure the uniformity of microbial reaction enrichment during the expansion process;

[0085] During the process of microbial strain expansion, the staff can perform synchronous monitoring through the controller 301. At this time, the controller 301 will display real-time data, including the values ​​of parameters such as temperature, pH, dissolved oxygen and pressure in the expansion reaction cylinder during the expansion process; use the indicator light 302 and the sensor to monitor the environmental changes in the expansion reaction cylinder 101 and adjust the relevant parameters in time to ensure that the expansion process is carried out under the best conditions; after the expansion is completed, the controller 301 turns off the electric device 103 and the stirring component 104; then the expanded bacterial liquid is output to the bacterial liquid storage barrel through the bacterial liquid output port 1011 and the liquid outlet faucet 105, and stored in the bacterial liquid storage cabinet in the cabinet or directly used for mixing with pig feed; at this time, the control mechanism 3 saves all data in the expansion process for subsequent analysis and optimization.

[0086] Example 2

[0087] like Figure 1-5 As shown, this embodiment provides a microbial expansion device, and the technical solution is similar to that of embodiment 1, except that:

[0088] The supply mechanism 2 also includes a pressurized water pump. A hot water delivery port 1021 is provided on the cylinder cover 102. After the hot water is heated by the water heater 2011, the pressurized water pump delivers the hot water used for microbial expansion through a delivery pipeline from the hot water delivery port 1021 to the inside of the expansion reaction cylinder 101.

[0089] Two water heaters 2011 are provided, and a series two-stage heating system is used to heat a portion of the reaction water through the first water heater, and then the water heated by the first water heater is heated to 40° C.-42° C. through the second water heater. A water flow sensor is provided in the water heater 2011 to monitor and measure the heating process and the flow of hot water injected into the expansion reaction cylinder 101.

[0090] The hot water supply control during the expansion process uses a series-type secondary heating system, combined with a temperature sensor to accurately control the required water temperature in the expansion reaction tube 101, providing a stable expansion environment; at the same time, heat loss is reduced. The use of a pressurized water pump ensures the pressure of the hot water during the transportation process, reduces heat loss, and ensures that the water for expansion maintains the required temperature when it reaches the reaction tube.

[0091] The water flow sensor can monitor and measure the heating process and the flow of hot water injected into the expansion reaction cylinder 101, ensuring the accuracy of the water volume and avoiding the expansion effect being affected by insufficient or excessive water. At the same time, it can realize real-time adjustment, and adjust the hot water delivery volume and temperature in real time according to the data fed back by the sensor to ensure the efficiency and stability of the expansion process.

[0092] Example 3

[0093] like Figure 1-5As shown, this embodiment provides another microorganism expansion culture device and expansion culture method thereof, and the technical scheme is similar to that of embodiment 1, wherein the difference is that:

[0094] A rotatable high-pressure washing nozzle 1012 and a water supply pipe port connected to the high-pressure washing nozzle 1012 are provided on the circumference of the cylinder wall of the expansion reaction cylinder 101, and the water supply pipe port is connected to the water inlet of the high-pressure washing nozzle 1012; a threaded ball capable of controlling the rotation angle is provided at the rotating head of the high-pressure washing nozzle 1012, and a nozzle is provided at the end of the nozzle;

[0095] The hot water supply component 201 delivers hot water to the high-pressure washing nozzle through the water supply pipe port; it is used to clean the inside of the expansion reaction cylinder 101 after the expansion of the bacterial strain is completed;

[0096] A waterproof camera is provided on the inner side panel of the cylinder cover 102 for monitoring the stirring and cleaning conditions inside the cylinder. The waterproof camera is electrically connected to the controller 301.

[0097] The expansion and cultivation reaction barrel 101 also includes an ultraviolet sterilization light belt; the expansion and cultivation reaction barrel 101 is evenly provided with indented grooves on the circumference of the barrel wall, and the ultraviolet sterilization light belt is embedded in the indented grooves. The ultraviolet sterilization light belt is electrically connected to the controller 301 and is used to remove the residual bacteria after the internal cleaning of the expansion and cultivation reaction barrel 101. The sealing of the indented groove is made of acrylic that can prevent bacterial growth, while achieving effective transmission of ultraviolet rays without affecting the disinfection function of the ultraviolet lamp. The rotatable high-pressure flushing nozzle 1012 is convenient for cleaning the equipment after expansion and cultivation, and the ultraviolet sterilization light belt arranged around the inner wall of the barrel is convenient for the overall disinfection of the equipment after expansion and cultivation.

[0098] Drying fans 4 are provided on both sides of the inner wall of the cabinet of the microorganism cultivation device to adjust the humidity of the gas inside the cabinet of the cultivation reaction cylinder 101 during the cultivation process;

[0099] A layer of graphene heating film is laid on the bottom plate of the layered rack; the graphene heating film is electrically connected to the control mechanism 3, and the drying fan 4 is also used to accelerate the air circulation after heating in the cabinet. The graphene heating film has excellent thermal conductivity and can achieve uniform heating in a short time; the graphene heating film laid on the bottom plate of the layered rack combined with the drying fan can quickly and evenly transfer heat to the bottom of the expansion reaction cylinder 101 and its surrounding area, ensuring that the temperature of the entire expansion environment is stable and consistent, thereby providing ideal temperature conditions for microbial cultivation.

[0100] The inner wall of the cabinet is also provided with a heating device for maintaining a constant temperature of the culture reaction tube 101, and the heating device is electrically connected to the control mechanism 3; the inner wall of the cabinet is also provided with heat-insulating cotton for reducing heat dissipation and reducing energy consumption of the device.

[0101] The controller 301 controls the high-pressure flushing nozzle 1012 to clean the inside of the expansion and cultivation reaction tube 101 by rotating at a certain angle to ensure that there is no residual bacterial liquid inside, and transmits the picture to the display screen of the controller 301 through the waterproof camera to view the internal cleaning status; the cleaning water after cleaning is discharged from the equipment through the bacterial liquid output port 1011 and the liquid outlet faucet 105, and stored in the bacterial liquid storage barrel; the staff turns on the ultraviolet sterilization light strip on the wall of the expansion and cultivation reaction tube 101 through the controller 301 to remove the residual bacteria after cleaning the inside of the expansion and cultivation reaction tube 101, so as to avoid infection of new bacterial liquid during subsequent expansion and cultivation of other bacteria.

[0102] The microbial expansion method using the microbial expansion device of this embodiment comprises the following steps:

[0103] S1: The staff first confirms the cleanliness and sterility of the inside and outside of the expansion and cultivation device, and checks through the control mechanism 3 and the waterproof camera to ensure that the equipment is in normal working condition; at the same time, confirm the material delivery ratio;

[0104] S2: Add materials, and put the bacteria species and culture materials required for expansion into the expansion reaction cylinder 101 through the supply mechanism 2;

[0105] S3: The control mechanism 3 starts the supply mechanism 2 to inject the water required for the expansion into the expansion reaction cylinder 101 through the cylinder cover 102;

[0106] S4: The control mechanism 3 turns on the electric device 103 and the stirring assembly 104 to work;

[0107] S5: The staff monitors and controls the temperature, pH, dissolved oxygen, pressure, and humidity in the expansion reaction cylinder 101 during the expansion process through the waterproof camera and the control mechanism 3;

[0108] S6: After the microbial expansion device is completed, the bacterial liquid is obtained. The staff cleans and disinfects the device through the control mechanism 3. After the high-pressure flushing nozzle 1012 cleans the equipment, the cleaning waste water is discharged through the liquid outlet faucet 105; finally, the ultraviolet sterilization light belt is used to disinfect the entire equipment of the expansion reaction cylinder.

[0109] More specific implementation steps are:

[0110] The staff first confirms the cleanliness and sterility of the inside and outside of the expansion device, and at the same time checks that the device and all sensors are in normal working condition through the control mechanism 3 to ensure that the device is working properly;

[0111] When adding raw materials, the staff confirms in advance that the amount added meets the preset expansion formula, then opens the cylinder cover 102, and adds the microbial strains and culture materials (such as syrup, etc.) that need to be expanded into the expansion reaction cylinder 101 through the feeding window 202 and the feeding port 1022;

[0112] After the expansion process begins, the supply mechanism 2 delivers the raw materials required for expansion and the preheated expansion water to the expansion reaction cylinder through the feeding port 1022 on the cylinder cover; the hot water supply component 201 provides heating water suitable for the expansion reaction, and the polyurethane foam insulation sleeve wrapped around the delivery pipeline ensures the stability of the hot water temperature; during the expansion process, the electric device 103 drives the stirring component 104 to stir in the expansion reaction cylinder to ensure that the expansion raw materials are evenly mixed and improve the cultivation effect.

[0113] The control mechanism 3 monitors the temperature, pressure and humidity of the expansion reaction in real time, and controls the environmental parameters in the reaction tube by adjusting the graphene heating film and the drying fan 4 to ensure that the microorganisms grow and expand under optimal conditions.

[0114] After the expansion and cultivation is completed, the conical design at the bottom of the expansion and cultivation reaction cylinder allows the cultured bacterial liquid to be smoothly discharged through the bacterial liquid outlet 1011, and introduced into the liquid outlet faucet 105 through the connected delivery pipe, and finally enters the bacterial liquid barrel for collection and storage. After the bacterial liquid is discharged, the high-pressure flushing nozzle 1012 in the expansion and cultivation reaction cylinder is started to thoroughly clean the inside of the cylinder; the rotation angle of the nozzle is controlled by the internal threaded ball to ensure that the cleaning covers every corner of the cylinder; the hot water provided by the hot water supply component enters the high-pressure flushing nozzle through the water supply pipe port, further improving the cleaning effect.

[0115] After cleaning, the ultraviolet sterilization light strip embedded in the recessed groove of the expansion reaction cylinder wall is started to sterilize the cylinder with ultraviolet light to remove residual microorganisms, ensure the sterility of the expansion device, and prepare for the next round of expansion. At this time, the drying fan circulates the heated airflow to quickly dry the cylinder, prevent mold growth, and further maintain the cleanliness and sterility of the equipment.

[0116] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the embodiments here. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the claims of the present invention.

Claims

1. A microbial culture device, characterized in that: It comprises a cabinet body, in which a culture expansion reaction mechanism (1) and a supply mechanism (2) are arranged; The expansion and cultivation reaction mechanism (1) comprises an expansion and cultivation reaction cylinder (101) and a cylinder cover plate (102) for sealing the expansion and cultivation reaction cylinder (101); the expansion and cultivation reaction cylinder (101) is fixedly connected to the cabinet; The expansion reaction mechanism (1) further comprises an electric device (103) and a stirring assembly (104); the cylinder cover plate (102) is connected to the electric device (103); the power output end of the electric device (103) is connected to the stirring assembly (104) through the cylinder cover plate (102); the stirring assembly (104) is arranged inside the expansion reaction cylinder (101); The supply mechanism (2) is connected to the cylinder cover plate (102) and is used to add raw materials required for the expansion reaction cylinder (101) to carry out expansion. The supply mechanism (2) comprises a hot water supply component (201), and the hot water supply component (201) is connected to the cylinder cover plate (102); The microorganism expansion device is also provided with a control mechanism (3) for monitoring and regulating the expansion reaction mechanism (1) and the supply mechanism (2); the control mechanism (3) is electrically connected to the expansion reaction mechanism (1) and the supply mechanism (2), respectively.

2. The microorganism expansion and cultivation device according to claim 1, characterized in that: The supply mechanism (2) further comprises a feeding window (202); the cylinder cover plate (102) is provided with a feeding port (1022) connected to the feeding window (202); The hot water supply component (201) comprises a water heater (2011) and a delivery pipeline for delivering water required for the reaction; the pipe body of the delivery pipeline is wrapped with a layer of polyurethane foam insulation sleeve; the delivery pipeline heats the incoming water through the water heater (2011), and then delivers the heated water required for the expansion and cultivation to the expansion and cultivation reaction cylinder (101) through the delivery pipeline; The heater storage cabinet is provided on the obliquely lower side of the cabinet layer where the expansion reaction cylinder (101) is located, and the water heater (2011) is fixedly installed on the inner wall of the cabinet of the heater storage cabinet; The cylinder cover plate (102) is also provided with a negative pressure regulating valve for regulating the pressure of the internal expansion process according to the type of cultured bacteria; the negative pressure regulating valve is arranged around the circumference of the panel of the cylinder cover plate (102); The cylinder cover plate (102) is provided with a sealing strip groove, in which a sealing ring strip is installed. The expansion reaction cylinder (101) is sealedly connected to the cylinder cover plate (102) via the sealing strip groove on the cylinder cover plate (102).

3. The microorganism expansion and cultivation device according to claim 2, characterized in that: The control mechanism (3) comprises a controller (301), an indicator light (302) and a sensor; The indicator light (302) and the sensor are electrically connected to the controller (301); The sensors include a temperature sensor, a pH sensor, a dissolved oxygen sensor and a pressure sensor arranged in the culture expansion reaction cylinder (101), and also include a humidity sensor arranged on the inner wall of the cabinet of the microorganism culture expansion device; The sensor is used to monitor and determine changes in temperature parameters, pH parameters, dissolved oxygen parameters and pressure parameters during the bacterial liquid expansion process inside the expansion reaction cylinder (101); the humidity sensor is used to monitor and determine changes in the humidity of the gas inside the cabinet.

4. The microorganism expansion and cultivation device according to claim 2, characterized in that: The bottom of the expansion culture reaction cylinder (101) is set to be conical; the conical transition part of the cylinder of the expansion culture reaction cylinder (101) is clamped and fixed on the layered frame built into the cabinet; The conical bottom of the culture expansion reaction cylinder (101) is provided with a bacterial liquid outlet (1011); the reaction mechanism (1) also includes a liquid outlet faucet (105), and the bacterial liquid outlet (1011) is connected to the liquid outlet faucet (105) through a delivery pipeline; a bacterial liquid barrel for loading bacterial liquid is placed below the outlet of the liquid outlet faucet (105); the microbial culture expansion device is also provided with a bacterial liquid storage cabinet for placing the bacterial liquid barrel; the bacterial liquid storage cabinet is arranged at the other side of the cabinet layer obliquely below the culture expansion reaction cylinder (101).

5. The microorganism expansion and cultivation device according to claim 4, characterized in that: A rotatable high-pressure flushing nozzle (1012) and a water supply pipe port connected to the high-pressure flushing nozzle (1012) are provided on the circumference of the cylinder wall of the expansion reaction cylinder (101), and the water supply pipe port is connected to the water inlet of the high-pressure flushing nozzle (1012); a threaded ball capable of controlling the rotation angle is provided at the rotating head of the high-pressure flushing nozzle (1012), and a nozzle is provided at the end of the nozzle; The hot water supply component (201) transmits hot water to the high-pressure washing nozzle through the water supply pipe port, so as to clean the inside of the culture expansion reaction cylinder (101) after the culture expansion is completed; A waterproof camera for monitoring the stirring and cleaning conditions inside the cylinder is provided on the inner side panel of the cylinder cover (102), and the waterproof camera is electrically connected to the controller (301). The expansion culture reaction cylinder (101) also includes an ultraviolet sterilization light strip; the expansion culture reaction cylinder (101) has sunken grooves evenly distributed on the circumference of the cylinder wall, and the ultraviolet sterilization light strip is embedded in the sunken grooves. The ultraviolet sterilization light strip is electrically connected to the controller (301) and is used to remove residual bacteria after cleaning the inside of the expansion culture reaction cylinder (101).

6. The microorganism expansion and cultivation device according to claim 5, characterized in that: Drying fans (4) are provided on both sides of the inner wall of the cabinet of the microorganism cultivation device to adjust the humidity of the gas inside the cabinet of the cultivation reaction cylinder (101) during the cultivation process; A layer of graphene heating film is laid on the bottom plate of the layered rack; the graphene heating film is electrically connected to the control mechanism (3), and the drying fan (4) is also used to accelerate the circulation of the heated air flow in the cabinet; The inner wall of the cabinet is also provided with a heating device for maintaining a constant temperature on the culture reaction cylinder (101), and the heating device is electrically connected to the control mechanism (3); the inner wall of the cabinet is also provided with heat-insulating cotton for reducing heat dissipation and reducing energy consumption of the device.

7. The microorganism expansion and cultivation device according to claim 2, characterized in that: The supply mechanism (2) also includes a pressurized water pump. A hot water delivery port (1021) is provided on the cylinder cover (102). After the hot water is heated by the water heater (2011), the pressurized water pump delivers the hot water for reaction used for microbial expansion through a delivery pipeline from the hot water delivery port (1021) to the inside of the expansion reaction cylinder (101).

8. The microorganism expansion and cultivation device according to claim 2, characterized in that: Two water heaters (2011) are provided, and a series two-stage heating system is adopted to first heat a part of the reaction water through the first water heater, and then the water heated by the first water heater is heated to 40°C-42°C through the second water heater.

9. The microorganism expansion and cultivation device according to claim 2, characterized in that: The water heater (2011) is provided with a water flow sensor for monitoring and measuring the heating process and the flow of hot water injected into the expansion reaction cylinder (101).

10. A microorganism expansion method using the microorganism expansion device according to claim 6, characterized in that: The following steps are involved: S1: The staff first confirms the cleanliness and sterility of the inside and outside of the expansion device, and checks through the control mechanism (3) and the waterproof camera to ensure that the equipment is in normal working condition; at the same time, confirm the material input ratio; S2: adding materials, and feeding the bacteria species and culture materials required for expansion culture into the expansion culture reaction cylinder (101) through the supply mechanism (2); S3: the control mechanism (3) starts the supply mechanism (2) to inject water required for the expansion culture into the expansion culture reaction cylinder (101) through the cylinder cover plate (102); S4: the control mechanism (3) turns on the electric device (103) and the stirring assembly (104) to start working; S5: The staff monitors and controls the changes in the temperature, pH, dissolved oxygen, pressure and humidity in the expansion and cultivation reaction cylinder (101) through the waterproof camera and the control mechanism (3) during the expansion and cultivation process; S6: After the microbial expansion device is completed, bacterial liquid is obtained. The staff cleans and disinfects the device through the control mechanism (3). After the high-pressure washing nozzle (1012) cleans the equipment, the cleaning waste water is discharged through the liquid outlet faucet (105); finally, the ultraviolet sterilization light strip is used to disinfect the entire expansion reaction tube.

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