Denitrification process and system for high-concentration nitrogen-containing sewage

Abstract

The invention discloses a denitrification system for high-concentration nitrogen-containing sewage. The denitrification system comprises a charging tank, an aeration pump, a reactor and a real-time detection control device, the charging tank is communicated with the reactor and is controlled by a valve; the aeration pump is used for controlling the conversion between nitration reaction and denitrification reaction; the reactor is filled with a filler which is adsorbed with mycobacteria with the preservation number of CCTCC (China Center for Type Culture Collection) M20241347; the real-time detection control device is used for detecting the ammonia nitrogen concentration, the nitrite concentration and the pH value of reaction liquid in the reactor, and controlling the aeration pump to be turned on and turned off on the basis of the detected ammonia nitrogen concentration, the detected nitrite concentration and the detected pH value of the reaction liquid in the reactor so as to realize conversion between nitration reaction and denitrification reaction; and controlling the opening and closing of the valve so as to control the addition of the organic carbon source, the acid or the alkali in the charging tank to the reactor. The invention further discloses a denitrification process of the high-concentration nitrogen-containing sewage, and efficient denitrification of the high-concentration nitrogen-containing sewage can be realized.

Description

Technical Field

[0001] The present application relates to the technical field of sewage treatment, and in particular to a denitrification process and system for high-concentration nitrogen-containing sewage. Background Art

[0002] The treatment of high-concentration nitrogen-containing wastewater represented by landfill leachate has always been the focus and difficulty in the field of wastewater treatment. High-concentration nitrogen-containing wastewater is difficult to treat biochemically and has high costs due to its complex composition, high ammonia nitrogen content (concentration is usually above 2000PPM), and relatively low content of easily degradable organic matter (C / N ratio is generally less than 2). At present, the treatment technologies for high-concentration nitrogen-containing wastewater mainly include membrane treatment and biological denitrification. Membrane treatment is only physical filtration, and does not really convert the main pollutants such as ammonia nitrogen, nitrite nitrogen and nitrate nitrogen in the sewage into harmless nitrogen gas and release it back into the atmosphere. To truly achieve this, we still have to rely on the link of biological denitrification.

[0003] The biological denitrification process currently in common use mainly consists of two steps: nitrification and denitrification. That is, ammonia nitrogen is first converted into nitrite nitrogen and nitrate nitrogen through nitrification reaction, and then nitrite nitrogen and nitrate nitrogen are converted into nitrogen gas through denitrification reaction. Considering that common nitrifying bacteria have low tolerance to high concentrations of ammonia nitrogen, the biological denitrification process currently in common use first dilutes nitrogen-containing wastewater and refluxes the nitrified liquid for denitrification to reduce the concentration of ammonia nitrogen and COD in the reaction liquid entering the nitrification tank. The denitrification efficiency (i.e., ammonia nitrogen load per unit volume) of the current biological denitrification system is generally below 180PPM / 24H, and the carbon source consumption per ton of raw water is generally above 10kg. The denitrification efficiency is low and the cost is high. Summary of the invention

[0004] In order to achieve efficient denitrification of high-concentration nitrogen-containing wastewater, this application proposes a denitrification process and system for high-concentration nitrogen-containing wastewater, and adopts the following technical solutions:

[0005] In one aspect, the present application discloses a denitrification system for high-concentration nitrogen-containing wastewater, the denitrification system comprising a charging tank, an aeration pump, a reactor, and a real-time detection and control device;

[0006] The charging tank is connected to the reactor and controlled by a valve;

[0007] The aeration pump controls the conversion between nitrification and denitrification;

[0008] The reactor is filled with a filler adsorbed with mycobacterium with a deposit number of CCTCC M 20241347;

[0009] The real-time detection and control device is used to detect the ammonia nitrogen concentration, nitrite concentration and pH value of the reaction liquid in the reactor, and based on the detected ammonia nitrogen concentration, nitrite concentration and pH value of the reaction liquid in the reactor, control the switch of the aeration pump to achieve the conversion of nitrification reaction and denitrification reaction; control the switch of the valve to achieve the addition control of the organic carbon source, acid or alkali in the charging tank to the reactor.

[0010] In a specific embodiment, the real-time detection and control device compares the ammonia nitrogen concentration of the reaction liquid with a first preset value and the nitrite concentration of the reaction liquid with a second preset value during the nitrification reaction stage, and issues an instruction to stop aeration; the real-time detection and control device compares the nitrite concentration of the reaction liquid with a third preset value and the ammonia nitrogen concentration of the reaction liquid with the first preset value during the denitrification reaction stage, and issues an instruction to discharge the reaction liquid from the system; the real-time detection and control device calculates the amount of organic carbon source required to complete the denitrification reaction based on the initial nitrite concentration at the start of the denitrification reaction during the denitrification reaction stage, and issues an instruction to supply the organic carbon source into the reactor.

[0011] In a specific embodiment, a water pump may be provided at the reaction liquid outlet of the reactor to discharge the reaction liquid out of the system.

[0012] In a specific embodiment, in the reactor, the filler adsorbed with mycobacterium with the deposit number of CCTCC M 20241347 occupies more than 40% of the volume of the reactor.

[0013] On the other hand, the present application discloses a denitrification process for high-concentration nitrogen-containing wastewater, which is operated in the above-mentioned denitrification system for high-concentration nitrogen-containing wastewater, comprising:

[0014] Inject nitrogen-containing wastewater (with an ammonia nitrogen concentration of 500-4000 PPM and a COD concentration of 500-5000 PPM) into the reactor;

[0015] Alternating aerated nitrification and anoxic denitrification (using a single reactor mode, i.e., nitrification and denitrification are carried out alternately in the same reactor, and aerated nitrification is carried out first);

[0016] During the nitrification reaction, the ammonia nitrogen concentration and the nitrite concentration of the reaction liquid in the reactor are detected once every period of time (1 hour or other time period), until the ammonia nitrogen concentration of the reaction liquid drops to a first preset value, or the nitrite concentration rises to a second preset value, then the current round of nitrification is stopped, that is, aeration is stopped;

[0017] Then, according to the nitrite concentration detected when the nitrification reaction stops, an appropriate amount of organic carbon source is added to the nitrification solution to carry out anoxic denitrification reaction;

[0018] During denitrification, the ammonia nitrogen concentration and the nitrite concentration of the reaction liquid in the reactor are detected once every period of time (1 hour or other time period) until the nitrite concentration of the reaction liquid drops to the third preset value, then the current round of denitrification is stopped; if the ammonia nitrogen concentration of the reaction liquid has not reached the first preset value at this time, aeration is restarted to enter the next round of nitrification reaction; if the ammonia nitrogen concentration of the reaction liquid has reached the first preset value at this time, then the current denitrification treatment is terminated.

[0019] Optionally, the pH value range of the aerated nitrification reaction and the denitrification reaction is 7-9.

[0020] Optionally, the first preset value ranges from 0 to 50 PPM.

[0021] Optionally, the second preset value ranges from 1000 to 1800 PPM.

[0022] Optionally, the third preset value ranges from 0 to 30 PPM.

[0023] In the present invention, the filler adsorbed with mycobacterium with a deposit number of CCTCC M 20241347 is prepared in the following manner: a suspension of mycobacteria with a deposit number of CCTCC M 20241347 is solidified on a filler (the filler includes but is not limited to polyester sponge, diatomaceous earth, chitosan, activated sludge and other materials that can be used as fillers) by adsorption.

[0024] Based on the above technical solution, the beneficial effects of the present application compared with the prior art include:

[0025] 1. The denitrification process in the embodiment of the present application, by pre-setting a high-tolerance denitrifying bacteria agent in the reactor, for nitrogen-containing sewage with ammonia nitrogen concentration and COD concentration above 500PPM, there is no need to dilute to reduce the ammonia nitrogen concentration, nor to perform a pre-denitrification reaction to reduce the COD concentration, and it can be directly injected into the reactor for aeration nitrification reaction. Since the high-tolerance denitrifying bacteria agent can withstand the impact of high-concentration nitrogen-containing sewage above 500PPM, the ammonia nitrogen concentration and COD concentration of the nitrogen-containing sewage in the reactor can be maintained at a high level of more than 500PPM for a long time, thereby reducing the inhibitory effect of high-concentration nitrogen-containing sewage on the denitrifying bacteria agent and improving the denitrification efficiency. And further, the unit tank capacity treatment capacity of the entire denitrification system (i.e., the system ammonia nitrogen load PPM / 24H) is greatly improved, which greatly saves the initial construction investment, floor space and daily processing average energy consumption of the entire system.

[0026] 2. The denitrification process in the embodiment of the present application can reduce the ammonia nitrogen concentration in the sewage after denitrification to below 30PPM, which is close to the discharge standard, and is conducive to the efficient and deep denitrification of high-concentration nitrogen-containing sewage. The tail water treated by this process can be discharged directly in compliance with the standard, completely eliminating the subsequent membrane treatment process; or the water production rate can be greatly improved and the service life of the membrane material can be extended after the membrane treatment process. Compared with the current mainstream process that relies heavily on membrane treatment, it can greatly reduce investment and operating costs (the membrane itself is a consumable and the energy consumption of membrane treatment is very high), and reduce secondary pollution caused by scrapped membrane materials.

[0027] 3. The denitrification system in the embodiment of the present application realizes precise and intelligent control of the nitrification and denitrification reaction processes by setting a real-time detection and control device. By real-time detection of the ammonia nitrogen concentration in the nitrification solution and the nitrite concentration in the denitrification reaction, excessive nitrification and excessive addition of carbon sources are avoided, thereby further reducing energy consumption and significantly saving carbon sources, effectively reducing processing costs. At the same time, it also avoids the increase in sludge production caused by the excessive growth of bacteria caused by the organic carbon source added in the denitrification section in the traditional process penetrating into the nitrification section, thereby greatly reducing the cost of residual sludge treatment.

[0028] 4. The denitrification system in the embodiment of the present application makes it possible to automate and cluster the processing system. The miniaturization and intensification of the system greatly saves space occupation and can achieve faster and more extensive distributed deployment. At the same time, the clustered design can not only maintain precise process control under large-volume processing conditions, but also achieve linear and smooth scale expansion or reduction, which is suitable for more and wider application scenarios.

[0029] Biological Deposit

[0030] The mycobacterium (Mycobacterium sp. TSHB-H01) provided by the present invention was deposited in the China Center for Type Culture Collection (on the campus of Wuhan University, No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province) on July 1, 2024. The abbreviation of the preservation unit is CCTCC, and the preservation number of the strain is CCTCC M 20241347. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 Schematic diagram of a denitrification system for high-concentration nitrogen-containing wastewater in an embodiment of the present application;

[0032] Figure 2 It is a process flow chart of denitrification of high-concentration nitrogen-containing wastewater in the embodiment of the present application.

[0033] Description of reference numerals:

[0034] 1. Loading tank; 2. Valve; 3. Unit controller; 4. Communication module; 5. Aeration pump; 6. Reactor;

[0035] 7. Sensor; 8. Water pump; 9. Detector; 10. Filler adsorbed with highly resistant denitrifying bacteria. DETAILED DESCRIPTION

[0036] The exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. Although the exemplary embodiments of the present disclosure are shown in the accompanying drawings, it should be understood that the present disclosure can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided to enable a more thorough understanding of the present disclosure and to fully convey the scope of the present disclosure to those skilled in the art.

[0037] Exemplary embodiments will be described in detail herein, examples of which are shown in the accompanying drawings. When the following description refers to the drawings, the same numbers in different drawings represent the same or similar elements unless otherwise indicated. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with the present disclosure. Instead, they are merely examples of devices and methods consistent with some aspects of the present disclosure as detailed in the appended claims.

[0038] In the description of the present disclosure, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present disclosure and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation of the present disclosure. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0039] In the description of the present disclosure, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present disclosure can be understood according to specific circumstances.

[0040] The inventors have made many attempts with reference to the prior art and found that the existing sewage denitrification process cannot effectively treat sewage with an initial ammonia nitrogen concentration of more than 300 PPM, and can only dilute the nitrogen-containing sewage several times or even more than ten times before treatment. Dilution causes a significant increase in the required pool capacity, coupled with the additional reaction pool capacity required for pre-denitrification, the unit pool capacity processing capacity of the entire system is greatly reduced. In addition, in the current biological denitrification process, the timing of the conversion from nitrification to denitrification is particularly important and has a significant impact on the overall efficiency cost. If the nitrification time is too short, the ammonia nitrogen is not fully degraded, and if the nitrification time is too long, too much nitrite will be further oxidized to nitric acid, which not only directly wastes the energy consumption of aeration, but also wastes more organic carbon sources in the denitrification stage. The current system is basically converted according to the fixed operating time based on the empirical value, and in view of the fact that the composition, temperature, and state of the microorganisms of the nitrogen-containing sewage are not fixed, the process conversion according to a fixed time is usually accompanied by huge waste.

[0041] Based on the above problems, the inventors conducted further research and development and made the present invention.

[0042] The present invention is described in detail below through specific embodiments:

[0043] Example 1

[0044] Reference Figure 1 The denitrification system in this embodiment includes three loading tanks 1, an aeration pump 5, a reactor 6 and a set of real-time detection and control devices. The reactor 6 has a tank capacity of 100L and is provided with a sewage inlet and a reaction liquid outlet. The reactor 6 is filled with a filler 10 adsorbed with mycobacteria with a deposit number of CCTCC M 20241347. The three loading tanks 1 are respectively connected to the reactor 6 and are respectively controlled by a valve 2. The aeration pump 5 controls the conversion between nitrification and denitrification reactions.

[0045] The real-time detection control device includes a detector 9, a sensor 7, a unit controller 3 and a communication module 4. The detector 9 is used to detect the ammonia nitrogen concentration and the nitrite concentration of the reaction liquid in the reactor 6, and send the detection results to the unit controller 3 for processing. The detector 9 can be any existing detection device that can detect the ammonia nitrogen concentration and the nitrite concentration. The sensor 7 monitors the pH value of the reaction liquid in the reactor 6 in real time, and sends the result to the unit controller 3 for processing.

[0046] The unit controller 3 controls the switch of the aeration pump 5 according to a preset control algorithm to realize the conversion between nitrification reaction and denitrification reaction; and controls the switch of the valve 2 to realize the addition control of the organic carbon source, acid and alkali in the charging tank 1.

[0047] In the denitrification system of this embodiment, a water pump 8 may be further provided at the reaction liquid outlet of the reactor 6 to discharge the reaction liquid out of the system.

[0048] In this embodiment, landfill leachate with an ammonia nitrogen concentration of 2600 PPM and a COD concentration of 3900 PPM is selected as nitrogen-containing wastewater to complete the denitrification process.

[0049] In the reactor 6 , the filler 10 adsorbed with mycobacterium with the deposit number of CCTCC M 20241347 occupies 40% of the volume of the reactor 6 .

[0050] The filler 10 adsorbed with mycobacterium with a deposit number of CCTCC M 20241347 is prepared in the following manner: a suspension of mycobacterium with a deposit number of CCTCC M 20241347 is solidified on a filler (polyester sponge particles) by adsorption.

[0051] Reference Figure 2 , run the denitrification process in the above denitrification system:

[0052] S100: 100L of nitrogen-containing wastewater with an ammonia nitrogen concentration of 2600PPM and a COD concentration of 3900PPM is injected into the reactor 6, and the aeration pump is turned on to perform an aeration nitrification reaction. The temperature of the reaction liquid is controlled between 20 and 37°C, and the pH of the aeration nitrification reaction is controlled between 7 and 9.

[0053] S200: After 36 hours, the ammonia nitrogen concentration of the reaction liquid in the reactor 6 drops below 1100 PPM and the nitrite concentration rises to 1500 PPM, as monitored by the detector 9. At this time, the aeration pump 5 is turned off, aeration is stopped, and the first round of nitrification reaction is stopped.

[0054] S300: The unit controller 3 controls the valve 2 to be opened, and 180 g of sodium acetate is added from a charging tank 1 to the reactor 6 to perform the first round of denitrification reaction. The pH of the denitrification reaction is controlled between 7 and 9.

[0055] S400: After 24 hours, the nitrite concentration of the reaction liquid in the reactor 6 drops below 30 PPM, and the ammonia nitrogen concentration is still above 1000 PPM, as monitored by the detector 9. At this time, the aeration pump 5 is turned on to restart aeration and perform a second round of nitrification reaction.

[0056] S500: After 28 hours, the ammonia nitrogen concentration of the reaction liquid in the reactor 6 drops below 500 PPM and the nitrite concentration rises to 1200 PPM as monitored by the detector 9. At this time, the aeration pump 5 is turned off, aeration is stopped, and the second round of nitrification reaction is stopped.

[0057] S600: The unit controller 3 controls the valve 2 to be opened, and 140 g of sodium acetate is added from a charging tank 1 to the reactor 6 to perform a second round of denitrification reaction. The pH of the denitrification reaction is controlled between 7 and 9.

[0058] S700: After 18 hours, the nitrite concentration of the reaction liquid in the reactor 6 drops below 30 PPM, and the ammonia nitrogen concentration is still above 400 PPM, as monitored by the detector 9. At this time, the aeration pump 5 is turned on to restart aeration and perform the third round of nitrification reaction.

[0059] S800: After 24 hours, the ammonia nitrogen concentration of the reaction liquid in the reactor 6 drops below 30 PPM and the nitrite concentration rises to 1200 PPM, as monitored by the detector 9. At this time, the aeration pump 5 is turned off, aeration is stopped, and the third round of nitrification reaction is stopped.

[0060] S900: The unit controller 3 controls the valve 2 to be opened, and 140 g of sodium acetate is added from the charging tank 1 to the reactor 6 to perform the third round of denitrification reaction. The pH of the denitrification reaction is controlled between 7 and 9.

[0061] S1000: After 18 hours, the nitrite concentration of the reaction liquid in the reactor 6 drops below 30 PPM and the ammonia nitrogen concentration also drops below 30 PPM through the monitoring of the detector. At this time, the denitrification is terminated and the water pump 8 is turned on to discharge the reaction liquid out of the system.

[0062] The denitrification system of this embodiment has a comprehensive denitrification efficiency (i.e., ammonia nitrogen load per unit volume) of 416PPM / 24H, and consumes 4.6kg of carbon source per ton of raw water. The specific calculation process is: the initial ammonia nitrogen concentration is 2600PPM, the final ammonia nitrogen concentration is 30PPM, and the difference is 2570; the total time is 148 hours, i.e., 6.17 days; so the average daily decrease of ammonia nitrogen is 416PPM. The cumulative addition of carbon source three times is 460g / 100L, so it is 4.6KG / ton of raw water.

[0063] Example 2

[0064] The denitrification system in this embodiment is different from that in Embodiment 1 in that a 1L beaker is used in this embodiment to replace the denitrification reactor in Embodiment 1.

[0065] The denitrification process of this embodiment includes the following steps S100-S600:

[0066] S100: A filler having adsorbed thereon a highly resistant denitrifying bacterial agent having a volume of about 400 ml is placed in a 1 L beaker.

[0067] The filler adsorbed with the highly resistant denitrifying bacteria is prepared in the following manner: the mycobacterium with the deposit number of CCTCCM20241347 is cultured in pure form, and the strain obtained by the pure culture is further processed to obtain a suspension, which is the highly resistant denitrifying bacteria. Then, the highly resistant denitrifying bacteria is solidified on the filler by adsorption.

[0068] S200: The leachate from a landfill in Wuhan was selected as the nitrogen-containing wastewater, with an initial ammonia nitrogen concentration of 2400 PPM and a COD concentration of 3500 PPM. 1L of the above nitrogen-containing wastewater was added to a beaker, and then the beaker was placed on a constant temperature laboratory table, the temperature was kept constant at 30°C, and aeration was performed with an aeration head, and the pH of the aeration nitrification reaction was controlled between 7 and 9.

[0069] S300: After 48 hours, the ammonia nitrogen concentration of the nitrification solution dropped to 800PPM, and the nitrite concentration rose to 1800PPM. At this time, the aeration was turned off to end the first round of nitrification; then 2g of sodium acetate was added to start the first round of denitrification;

[0070] S400: After 24 hours, the concentration of nitrite in the reaction solution is lower than 20PPM, and the first round of denitrification ends. At this time, aeration is turned on to start the second round of nitrification, and the pH is controlled between 7 and 9;

[0071] S500: After 30 hours, the ammonia nitrogen in the reaction solution is less than 30 PPM, and the nitrite is higher than 1500 PPM. At this time, the aeration is turned off to end the second round of nitrification. Then 1.8 g of sodium acetate is added to start the second round of denitrification.

[0072] S600: After 14 hours, the nitrite in the reaction liquid is lower than 30PPM, and the ammonia nitrogen is lower than 30PPM. This round of denitrification is completed and the water valve is opened to discharge the reaction liquid.

[0073] The denitrification system in this embodiment has a comprehensive denitrification efficiency of 490 PPM / 24H and consumes 3.8 g of carbon source per liter of raw water.

[0074] The above description includes examples of one or more embodiments. Of course, it is impossible to describe all possible combinations of components or methods for the purpose of describing the above embodiments, but it should be recognized by those skilled in the art that the various embodiments may be further combined and arranged. Therefore, the embodiments described herein are intended to cover all such changes, modifications and variations that fall within the scope of protection of the appended claims. In addition, with respect to the term "comprising" used in the specification or claims, the word is covered in a manner similar to the term "including", as explained by "including" used as a transitional word in the claims. In addition, any term "or" used in the specification of the claims is intended to mean "non-exclusive or".

Claims

1. A denitrification system for high-concentration nitrogen-containing wastewater, characterized in that: The denitrification system includes a charging tank, an aeration pump, a reactor and a real-time detection and control device; The charging tank is connected to the reactor and controlled by a valve; The aeration pump controls the conversion between nitrification and denitrification; The reactor is filled with a filler adsorbed with mycobacterium with a deposit number of CCTCC M 20241347; The real-time detection and control device is used to detect the ammonia nitrogen concentration, nitrite concentration and pH value of the reaction liquid in the reactor, and based on the detected ammonia nitrogen concentration, nitrite concentration and pH value of the reaction liquid in the reactor, control the switch of the aeration pump to achieve the conversion of nitrification reaction and denitrification reaction; control the switch of the valve to achieve the addition control of the organic carbon source, acid or alkali in the charging tank to the reactor.

2. The denitrification system for high-concentration nitrogen-containing wastewater according to claim 1, characterized in that: The real-time detection and control device compares the ammonia nitrogen concentration of the reaction liquid with the first preset value and the nitrite concentration of the reaction liquid with the second preset value during the nitrification reaction stage, and issues an instruction to stop aeration; the real-time detection and control device compares the nitrite concentration of the reaction liquid with the third preset value and the ammonia nitrogen concentration of the reaction liquid with the first preset value during the denitrification reaction stage, and issues an instruction to discharge the reaction liquid from the system; the real-time detection and control device calculates the amount of organic carbon source required to complete the denitrification reaction according to the initial nitrite concentration at the start of the denitrification reaction during the denitrification reaction stage, and issues an instruction to supply the organic carbon source into the reactor.

3. The denitrification system for high-concentration nitrogen-containing wastewater according to claim 1, characterized in that: A water pump can also be provided at the reaction liquid outlet of the reactor to discharge the reaction liquid out of the system.

4. The denitrification system for high-concentration nitrogen-containing wastewater according to claim 1, characterized in that: In the reactor, the filler adsorbed with mycobacterium with the deposit number of CCTCC M 20241347 accounts for more than 40% of the volume of the reactor.

5. A denitrification process for high-concentration nitrogen-containing wastewater, characterized in that: The method is operated in a denitrification system for high-concentration nitrogen-containing wastewater as described in any one of claims 1 to 4, comprising: injecting the nitrogen-containing wastewater into a reactor filled with a filler adsorbed with mycobacteria having a deposit number of CCTCC M 20241347; Alternate aeration nitrification and anoxic denitrification; When the nitrification reaction is in progress, the ammonia nitrogen concentration and the nitrite concentration of the reaction liquid in the reactor are detected at regular intervals until the ammonia nitrogen concentration of the reaction liquid drops to a first preset value, or the nitrite concentration rises to a second preset value, then the current round of nitrification is stopped, that is, aeration is stopped; Then, according to the nitrite concentration detected when the nitrification reaction stops, an appropriate amount of organic carbon source is added to the nitrification solution to carry out anoxic denitrification reaction; During denitrification, the ammonia nitrogen concentration and the nitrite concentration of the reaction liquid in the reactor are detected at regular intervals until the nitrite concentration of the reaction liquid drops to a third preset value, and then the current round of denitrification is stopped; if the ammonia nitrogen concentration of the reaction liquid has not reached the first preset value at this time, aeration is restarted to enter the next round of nitrification reaction; if the ammonia nitrogen concentration of the reaction liquid has reached the first preset value at this time, the current denitrification treatment is terminated.

6. The method according to claim 5, characterized in that The pH value range of the aeration nitrification reaction and the denitrification reaction is 7-9.

7. The method of claim 5, characterized in that The first preset value ranges from 0 to 50 PPM.

8. The method of claim 5, characterized in that The second preset value ranges from 1000 to 1800 PPM.

9. The method of claim 5, characterized in that The third preset value ranges from 0 to 30 PPM.

10. The method of claim 5, characterized in that The filler adsorbed with mycobacterium with a deposit number of CCTCC M 20241347 is prepared in the following manner: a suspension of mycobacterium with a deposit number of CCTCC M 20241347 is solidified on the filler by adsorption.

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