Hexaflumuron and chlorantraniliprole compound insecticide

Abstract

The invention provides a compound pesticide insecticide and application thereof.The compound pesticide insecticide is mainly composed of two active ingredients including hexaflumuron and chlorantraniliprole, after hexaflumuron and chlorantraniliprole are compounded according to the mass ratio of 1: 2, a synergistic effect can be achieved on cabbage beet armyworms, the co-toxicity coefficient can reach 151.73, the LC50 is 0.7320 mg / L, the LC90 is 4.2092 mg / L, and the pesticide effect is good. The compound pesticide has a good control effect on cabbage beet armyworms.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide compounding, and particularly relates to a compound insecticide of hexaflumuron and chlorfenapyr and application thereof. Technical Background

[0002] The beet armyworm belongs to the family Noctuidae of the order Lepidoptera. It is a worldwide agricultural pest with a mediocre diet and a wide host range. It likes to eat crops such as vegetables, soybeans, peanuts, corn and cotton. In recent years, the beet armyworm has caused serious damage in my country, especially causing huge losses to vegetables.

[0003] Fluflumuron is a benzoylurea insecticide and a chitin synthesis inhibitor. It mainly acts as a stomach poison, but also has a contact killing effect. It can inhibit the molting and feeding speed of insects after contact. It has strong knockdown power, rapid action, and also has an ovicidal effect. It is mainly used to control a variety of Coleoptera, Diptera, and Homoptera insects.

[0004] Chlorantraniliprole is a new generation of o-formylaminobenzamide insecticide with stomach poison, systemic and contact toxicity. It has good preventive effect on Lepidoptera pests of the family Plutella xylostella. Due to its new mechanism of action, it has become a better agent for pest resistance control and rotation use.

[0005] The cabbage beet armyworm is an important pest on cabbage. Long-term use of a single agent for prevention and control is prone to drug resistance. In order to effectively prevent and control the cabbage beet armyworm, the present invention uses hexaflumuron and chlorantraniliprole as main active ingredients to develop a compound pesticide insecticide with a synergistic effect on the cabbage beet armyworm. Summary of the invention

[0006] Hexaflumuron is a novel acyl urea insecticide, a chitin synthesis inhibitor, and has high insecticidal and ovicidal activity; chlorantraniliprole is an amide insecticide, which mainly acts as a stomach poison and also has a contact effect. The present invention uses hexaflumuron and chlorantraniliprole in combination, and the combination of the two has a synergistic effect on cabbage beet armyworm, and has an excellent control effect on cabbage beet armyworm.

[0007] A compound insecticide of hexaflumuron and chlorantraniliprole: the active ingredients of the pesticide insecticide consist of hexaflumuron and chlorantraniliprole, and the mass ratio of hexaflumuron to chlorantraniliprole is 1:2; the content of hexaflumuron in the pesticide insecticide is 4.8%, the content of chlorantraniliprole is 9.6%, the total active ingredient content in the preparation is 150 g / L, and the preparation dosage form is a suspension; the pesticide insecticide is mainly used for preventing and controlling cabbage beet armyworm.

[0008] The beneficial technical effect of the present invention is embodied in that: the present invention optimizes and screens the effective component ratio, and after compounding hexaflumuron and chlorantraniliprole in a mass ratio of 1:2, a synergistic effect is produced on cabbage beet armyworm, and the co-toxicity coefficient can reach 151.73, and the LC50 0.7320mg / L, LC 90 The content of hexaflumuron and chlorfenapyr is 4.2092 mg / L. The dosage of the compound insecticide is small and the cost is low, which will not affect the safety and yield of cabbage. DETAILED DESCRIPTION

[0009] The present invention will be further described below in conjunction with specific embodiments. The features and advantages of the present invention will become clearer as the description proceeds. However, the exemplary embodiments are only used to illustrate the present invention and do not constitute any limitation to the scope of the present invention.

[0010] Example 1

[0011] The leaf dipping method was used to determine the indoor toxicity of different mixture ratios of hexaflumuron and chlorfenapyr to Spodoptera exigua, providing an experimental basis for the field application of their mixture.

[0012] 1. Test conditions

[0013] 1.1 Test reagents

[0014] 95% fluazifop technical, provided by Dezhou Lvba Fine Chemical Co., Ltd.; 98% chlorfenapyr technical, provided by Shandong Youdao Chemical Co., Ltd.

[0015] 1.2 Other reagents

[0016] Acetone, Tween-80, etc.

[0017] 1.3 Test targets

[0018] Beet armyworm, the insect source was collected from the cabbage field in Gaoliu Town, Shushan District, Hefei City. The collected insect sources were brought back to the laboratory for artificial breeding, egg laying, hatching, and rearing for 3 generations without contact with pesticides. The second-instar larvae with uniform body color and size were selected for testing.

[0019] 1.4 Rearing conditions

[0020] The test insects were raised under the conditions of a temperature of (27±1)°C, light / darkness=12h / / 12h, and a humidity of 50% to 60%.

[0021] 1.5 Instruments and Equipment

[0022] Writing brush, volumetric flask, beaker, marker pen, stopwatch, tweezers, glass rod, electronic balance, 9 cm diameter culture dish, pipette, constant temperature insect breeding room, etc.

[0023] 2. Test methods

[0024] 2.1 Preparation of medicine

[0025] On the basis of the preliminary test, 0.0421g of 95% hexaflumuron technical drug and 0.0816g of 98% chlorfenapyr technical drug were accurately weighed with an electronic analytical balance, dissolved in acetone and fixed to 100mL volumetric flasks, respectively, to obtain 400mg / L hexaflumuron mother solution and 800mg / L chlorfenapyr mother solution. On the basis of the above mother solution, hexaflumuron was formulated into 4, 2, 1, 0.5, 0.25mg / L with 0.1% Tween 80 solution: first, 1ml of hexaflumuron mother solution was taken with a pipette, fixed to 100ml, to obtain 4mg / L hexaflumuron solution, and then diluted in half to 2, 1, 0.5, 0.25mg / L concentration gradient for standby use. Chlorantraniliprole was prepared into 8, 4, 2, 1, and 0.5 mg / L: first, 1 ml of chlorantraniliprole mother solution was taken with a pipette, and the volume was fixed to 100 ml to obtain 8 mg / L chlorantraniliprole solution, and then half-diluted into 4, 2, 1, and 0.5 mg / L concentration gradient for standby use. Five different ratios of hexaflumuron and chlorantraniliprole (the ratio of the active ingredients was 1:1; 1:2; 1:3; 1:4; 1:5) were first dissolved in acetone, and then diluted into 4, 2, 1, 0.5, and 0.25 mg / L series concentrations with a solution containing 0.1% Tween-80 for standby use. A 0.1% Tween 80 sterile aqueous solution was set as a blank control.

[0026] 2.2 Test methods

[0027] Refer to the leaf dipping method recommended by NY / T 1154.14-2008: Cut the cabbage leaves into 8cm diameter discs, immerse them in the above solutions, take them out and dry them after 10s, place them in a petri dish with moisturizing filter paper, and inoculate beet armyworms. Repeat 4 times for each treatment, with 20 beet armyworms per repeat, and set the corresponding 0.1% Tween 80 solution as a blank control. Raise them for 72h under the conditions of temperature (27±1)℃, light / / darkness=12h / / 12h, humidity 50%-60%, check the mortality rate, and the control mortality rate is less than 20% for effective determination.

[0028] 3. Data statistics and analysis

[0029] 3.1 Calculation method

[0030] Based on the survey data, the adjusted mortality rate of each treatment was calculated.

[0031] The adjusted mortality rate is calculated according to formula (1) and formula (2) in percentage (%), and the calculation result is rounded to two decimal places:

[0032]

[0033] Where: P1—mortality rate

[0034] K—Number of dead bugs

[0035] N—Total number of insects treated

[0036]

[0037] Where: P2—corrected mortality rate

[0038] P t — Dealing with mortality

[0039] P0—blank control mortality

[0040] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to formula (2); if the control mortality rate is greater than 20%, the test needs to be repeated.

[0041]

[0042] TTI of mixed agent (M) = TI of agent A × content of A in M ​​(%) + TI of agent B × content of B in M ​​(%)

[0043]

[0044] 3.2 Statistical analysis

[0045] DPS software was used for statistical analysis to find the toxicity regression line of the test agent (Y =

[0046] a+bx) and correlation coefficient (r), LC 50 , LC 90 value.

[0047] 3.3 Evaluation indicators of synergistic effect

[0048] According to the evaluation indicators in the Pesticide Indoor Bioassay Test Guidelines - Insecticides (Part 7: Determination of Combined Action of Mixtures) (NY / T1154.7-2006), when the co-toxicity coefficient (CTC)

[0049] When CTC ≥120, it is a synergistic effect; when CTC ≤80, it is an antagonistic effect; 80<CTC<120, it is an additive effect.

[0050] 4. Results Analysis and Conclusion

[0051] 4.1 Results Analysis

[0052] The toxicity of hexaflumuron, chlorantraniliprole and their mixtures to cabbage beet armyworm was tested by the above method and statistically analyzed. The results are shown in Table 1. The co-toxicity coefficients of the five different ratios to cabbage beet armyworm were all greater than 120, indicating that the combination of the two agents has a synergistic effect on cabbage beet armyworm. Among them, when the ratio of the active ingredients of hexaflumuron and chlorantraniliprole was 1:2, the co-toxicity coefficient was the highest, which was 151.73.

[0053] Table 1 Toxicity of different ratios of hexaflumuron and chlorantraniliprole to Spodoptera exigua

[0054]

[0055] 4.2 Conclusion

[0056] The results of indoor toxicity test showed that the co-toxicity coefficients of hexaflumuron and chlorantraniliprole in five different ratios against cabbage beet armyworm were all greater than 120, indicating that the combination of the two agents has a synergistic effect and is feasible and necessary for the control of cabbage beet armyworm. When hexaflumuron and chlorantraniliprole are used to control cabbage beet armyworm, the ratio of the active ingredients of hexaflumuron to chlorantraniliprole is selected to be 1:2 for the combination.

[0057] Example 2

[0058] Through field efficacy tests, the efficacy of 150 g / L flubendiamide·chlorfenapyr suspension in controlling cabbage beet armyworm and its safety to cabbage and other non-target organisms were evaluated, and the optimal dosage was determined.

[0059] 1. Test basis

[0060] The People's Republic of China's "Quality Management Standards for Pesticide Registration Tests", the agricultural industry standard "Quality Management Standards for Pesticide Registration Field Efficacy Tests" (NY / T 2885-2016), "Guidelines for Pesticide Field Efficacy Tests (I) Insecticides for Control of Lepidoptera Larvae in Cruciferous Vegetables" (GB / T 17980.13-2000), etc.

[0061] 2. Test environment

[0062] 2.1 Test site location

[0063] Wusidu Village, Xiaomiao Town, Hefei City, Anhui Province (N:31.89°E:117.01°).

[0064] 2.2 Test target situation

[0065] The target of the experimental control is the beet armyworm. Cruciferous vegetables and other vegetables are planted all year round around the experimental field, and the occurrence of beet armyworm is moderate.

[0066] 2.3 Experimental crops, varieties and growth conditions

[0067] Crop: 20 blue cabbage, variety: Jingfeng No. 1. The cultivation conditions such as cabbage variety, field density, fertilizer and water management in each plot are consistent, and the cabbage grows evenly.

[0068] 2.4 Experimental soil type

[0069] The soil type is loam, with an organic matter content of 2.32% and a pH of 6.5.

[0070] 2.5 Other drugs used in the test site

[0071] No other pesticides were used in the experimental field during the entire experimental period.

[0072] 2.6 Meteorological data

[0073] It was light rain to cloudy on the day of application. There was no rainfall during application and 24 hours after application. The measured temperature was 23.3℃, ​​humidity was 70.2%, wind was northeast, and the wind speed was 1.7m / s.

[0074] 3. Experimental Grouping

[0075] 3.1 Field efficacy test grouping

[0076] Table 2 Grouping of field efficacy test

[0077]

[0078] 3.2 Plot area and replication

[0079] The plots were arranged in random blocks, each plot was 15 square meters, there were 6 treatments in the experimental area, repeated 4 times, a total of 24 plots, isolation areas were set between the plots, and protection rows were set up around them.

[0080] 3.3 Application method

[0081] Spray the stems and leaves, starting from the blank control and spraying evenly in each plot from low concentration to high concentration.

[0082] 4. Survey methods, time and frequency

[0083] 4.1 Survey time and frequency

[0084] The insect population base was investigated before application, and the efficacy was investigated on the 2nd and 10th days after application, for a total of 3 investigations.

[0085] 4.2 Survey Methods

[0086] Five random survey points were taken in each plot, and the number of live larvae of different ages on two cabbages were surveyed at each point.

[0087] 4.3 Calculation method of drug efficacy The drug efficacy is calculated according to formula (1) and formula (2):

[0088]

[0089] 4.4 Direct impact on crops

[0090] On the 2nd and 10th day after drug application, the growth of cabbage in each treatment area and the blank control area was observed visually.

[0091] 4.5 Other biological impacts

[0092] On the 2nd and 10th day after application, the growth of other pests and diseases and other organisms in the treated area and the blank control area were observed visually.

[0093] 5. Test results

[0094] 5.1 Results of the cabbage beet armyworm control test

[0095] Table 3 Test results of 150 g / L fluphenazine·chlorfenapyr suspension against cabbage beet armyworm

[0096]

[0097] Note: The significance of this test was determined using the Duncan's new multiple range (DMRT) method, using DPS software.

[0098] On the second day after treatment, the control effects of 150 g / L hexaflumuron·chlorfenapyr suspension at active ingredient dosages of 22.5, 31.5, and 40.5 g / ha on cabbage beet armyworm were 69.76%, 70.09%, and 77.33%, respectively. The control effect increased with the increase of dosage, and the control effect of the high dosage (40.5 g / ha) of the test agent was significantly higher than that of the low and medium dosages, while there was no significant difference in control effect between the low (22.5 g / ha) and medium (31.5 g / ha) dosages; the control effect of the control agent 15% hexaflumuron water dispersible granules on cabbage beet armyworm was 58.23%, which was significantly lower than the control effects of the test agent at each dosage; the control effect of the control agent 5% chlorfenapyr suspension on cabbage beet armyworm was 72.10%, which was significantly lower than the control effect of the high dosage of the test agent, but there was no significant difference with the medium dosage of the test agent.

[0099] On the 10th day after application, the control effects of low, medium and high doses of 150 g / L fluflumuron·chlorfenapyr suspension on beet armyworm were 87.65%, 90.61% and 93.52% respectively, which were all higher than the control effects 2 days after application of each dose, and increased with the increase of application dose. The control effects of low, medium and high doses of the tested agents were significantly different; the control effect of 15% fluflumuron water dispersible granules on cabbage beet armyworm was 86.55%, which was significantly lower than the high and medium doses of the tested agents, but had no significant difference with the low dose; the control effect of 5% chlorfenapyr suspension on cabbage beet armyworm was 88.80%, which was significantly lower than the high dose of the tested agents, but had no significant difference with the medium and low doses of the tested agents.

[0100] The test results show that treatments with various dosages of 150 g / L fluphenazine·chlorfenapyr suspension have good control effects on cabbage looper, and the recommended dosage of active ingredient is 22.5-40.5 g / hectare (preparation dosage is 10-18 ml / mu).

[0101] 5.2 Direct impact on crops

[0102] The growth of cabbage in each treatment area was consistent with that in the control area, with no abnormal phenomena.

[0103] 5.3 Other biological impacts

[0104] There were no abnormalities in predatory natural enemies and other organisms in the drug-treated and control areas.

[0105] The above description is only a preferred embodiment of the present invention. It should be pointed out that, for ordinary technicians in this technical field, the content of the present invention can be improved and modified without departing from the technical principle of the present invention. These improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A compound insecticide of hexaflumuron and chlorantraniliprole, characterized in that: The active ingredients of the pesticide are hexaflumuron and chlorfenapyr, and the mass ratio of hexaflumuron to chlorfenapyr is 1:

2.

2. The compound insecticide of hexaflumuron and chlorfenapyr according to claim 1, characterized in that: The content of hexaflumuron in the pesticide is 4.8%, the content of chlorantraniliprole is 9.6%, the content of total active ingredients in the preparation is 150 g / L, and the preparation is in the form of a suspension.

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