A bacterial suspension preparation instrument for medical clinical microbiological detection
By designing a bacterial suspension preparation instrument for microbial detection, using disks, circular plates, rotating shafts and sleeves to realize the rotation and rotation of the test tubes, the problem of time-consuming and laborious preparation of bacterial suspension and inaccurate concentration in the prior art is solved, and the preparation efficiency and reliability of the detection results are improved.
Patent Information
- Application Number
- CN202411857616.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-17
- Publication Date
- 2025-03-14
- Estimated Expiration
- 2044-12-17
AI Technical Summary
In the prior art, preparation of bacterial suspensions in microbial detection is time-consuming and labor-intensive, and the operation is inflexible, resulting in inaccurate concentrations and affecting the test results.
A bacteria suspension preparation device for medical clinical microbial detection is designed. Through the arrangement of a disc, a first circular plate, a first rotation shaft, combined with a sleeve, a gear, a rubber ring and other structures, the rotation and rotation of the test tube are realized, and the mixing uniformity of the bacteria suspension and normal saline is improved.
It improves the efficiency and accuracy of bacterial suspension preparation, reduces the time and errors of manual operation, and ensures the reliability of microbial detection results.
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Figure CN119307357B_ABST
Abstract
Description
Technical Field
[0001] The present invention belongs to the technical field related to microbial detection, and more specifically, particularly relates to a bacterial suspension preparation instrument for medical clinical microbial detection. Background Art
[0002] Quality inspection departments in the public safety industry, such as food, medicine, cosmetics, health food, and water quality testing, often need to use quantitative microorganisms for culture medium suitability inspection, sensitivity inspection, antibacterial efficacy inspection, laboratory certification, positive control test, and method confirmation when conducting microbiological related tests. For example, the validation test of the drug microbiological inspection method requires more than 5 kinds of microorganisms to be prepared into bacterial suspensions of different concentrations. However, the bacterial suspension preparation instrument in the prior art has the following defects:
[0003] In the prior art, laboratory preparation of quantitative microorganisms is still mainly manual, which is time-consuming and labor-intensive, with poor reliability, and inaccurate concentration of bacterial suspensions often leads to invalid or erroneous test results;
[0004] In the prior art, bacterial suspension preparation instruments need to use sampling components to sample microbial samples during the microbial detection process in clinical medicine. However, the sampling process of the existing sampling components has poor operational flexibility and cannot uniformly and efficiently package samples according to actual sampling needs, thereby affecting the efficiency of bacterial suspension preparation.
[0005] Therefore, in view of this, the existing structure and deficiencies are studied and improved, and a bacterial suspension preparation instrument for medical clinical microbiological detection is provided, in order to achieve a more practical and valuable purpose. Summary of the invention
[0006] The present invention provides a bacterial suspension preparation instrument for medical clinical microbiological detection, which is used to overcome the above-mentioned defects in the prior art.
[0007] The purpose and efficacy of the bacterial suspension preparation instrument for medical clinical microbiological detection of the present invention are achieved by the following specific technical means:
[0008] A bacterial suspension preparation instrument for medical clinical microbial detection comprises a shell, a first fixing plate is fixedly provided on the lower inner side of the shell, a second fixing plate is fixedly provided on the upper inner side of the shell, a partition is fixedly connected between the first fixing plate and the second fixing plate, a sampling chamber and a preparation chamber are separated by the first fixing plate, the second fixing plate and the partition in the middle of the shell, an operating chamber is separated by the second fixing plate in the upper part of the shell, a sampling assembly is provided in the operating chamber, and a preparation assembly is provided in the preparation chamber; the preparation assembly comprises a first mounting plate, the first mounting plate is fixedly provided on the lower inner side of the preparation chamber, and the first A disc is rotatably provided on the upper side of the mounting plate, a first rotating shaft is fixedly provided in the middle of the disc, a first circular plate is fixedly provided on the upper end of the first rotating shaft, a plurality of sleeves are provided in a circumferential array on the disc, a first rubber ring is provided on the inner side of each sleeve, a plurality of second rubber rings are provided in a circumferential array on the first circular plate, a plurality of test tubes are placed in a circumferential array on the disc and the first circular plate, a plurality of fixed tubes are connected between the upper side of the disc and the lower side of the first circular plate, a push rod is slidably provided on one side of each of the fixed tubes, an arc plate is fixedly provided on one end of each of the push rods close to the test tube direction, and an arc rubber piece is provided on the inner side of each of the arc plates.
[0009] The inner side of each first rubber ring is in extrusion contact with the outer side of the lower part of the test tube, the inner side of each second rubber ring is in extrusion contact with the outer side of the upper part of the test tube, the inner side of each arc-shaped rubber member is in contact with the outer side of the middle part of the test tube, and a push rod is vertically slidably provided inside each fixed tube, and the upper end of each push rod is in sliding contact with the inclined surface of one end of the push rod.
[0010] A slider is fixedly provided on one side of each push rod, a spring is connected between each slider and the inner wall of the fixed tube, an annular guide block is fixedly provided on the upper side of the first mounting plate, the upper part of the annular guide block includes a first plane part, a second plane part, and two inclined parts, the first plane part of the annular guide block is lower than the second plane part of the annular guide block, the two ends of the first plane part of the annular guide block are respectively connected to the two ends of the second plane part of the annular guide block through the two inclined parts of the annular guide block, and the lower end of each push rod is in sliding contact with the upper part of the annular guide block.
[0011] A plurality of grooves are arranged at intervals on one side of the second plane portion of the annular guide block, and a wave-shaped uneven structure is formed on one side of the second plane portion of the annular guide block through the plurality of grooves. An annular elastic member is arranged on the upper side of the first mounting plate, and the lower end of each test tube is in sliding contact with the upper side of the annular elastic member.
[0012] A first annular plate is fixedly provided on the outer side of the middle part of each sleeve, and each of the first annular plates slides in annularly on the upper part of the disc, a first gear is fixedly provided on the outer side of the lower part of each sleeve, a fixing ring is fixedly provided on the upper side of the first mounting plate, and a second gear is fixedly provided on the upper end of the fixing ring, the outer side of each first gear is meshed with the outer side of the second gear, a second annular plate is fixedly provided on the outer side of each second rubber ring, each of the second annular plates slides in annularly inside the first circular plate, the outer side of each second rubber ring is in sliding contact with the inner side of the first circular plate, and the outer side of each sleeve is in sliding contact with the upper part of the disc.
[0013] A physiological saline tank is provided on one side of the upper portion of the preparation chamber, a first discharge pipe is provided at the lower end of the physiological saline tank, a turbidimeter is installed on one side of the lower portion of the preparation chamber, a first stepper motor is installed at the lower end of the first mounting plate, and an output end of the first stepper motor is fixedly connected to the lower end of the first rotating shaft.
[0014] A second mounting plate is fixedly provided inside the sampling chamber, a second circular plate is rotatably provided on the upper side of the second mounting plate, a plurality of limit shells are provided in a circular array on the upper part of the second circular plate, a fixed block is fixedly provided on the outer side of each limit shell, a telescopic rod is fixedly provided on the upper side of the second mounting plate, the telescopic rod is located on the outer side of the second circular plate, the extended end of the telescopic rod contacts the lower side of the fixed block, a second stepper motor is installed on the lower side of the second mounting plate, and the output end of the second stepper motor is fixedly connected to the second circular plate.
[0015] A solution box is provided on one side of the upper part of the sampling cavity, a second feeding pipe is provided on the lower side of the solution box, and a camera is installed on the upper part of the sampling cavity.
[0016] A first closed door is installed on the upper side of the operating chamber, a second closed door is installed on one side of the sampling chamber, a third closed door is installed on one side of the preparation chamber, a sterilization ultraviolet light lamp is installed on one side of the upper part of the operating chamber, a fan is provided on the other side of the upper part of the operating chamber, a plurality of control buttons are provided on one side of the lower part of the shell, and a display screen is installed on one side of the upper part of the shell.
[0017] The sampling assembly includes an electric telescopic rod, which is fixed on the upper side of the operating chamber. A movable plate is fixedly provided at the protruding end of the electric telescopic rod, and a third stepper motor is installed on the movable plate. A second rotating shaft is fixedly provided at the output end of the third stepper motor, and a third circular plate is fixedly provided at the lower end of the second rotating shaft. A plurality of placement holes are provided in a circular array on the third circular plate, and a pipette is placed inside each of the placement holes. The upper part of the pipette is limited on the upper side of the third circular plate.
[0018] Further technical solutions,
[0019] Compared with the prior art, the present invention has the following beneficial effects:
[0020] The present invention provides a bacterial suspension preparation instrument for medical clinical microbial detection. Through the arrangement of a disc, a first circular plate, and a first rotating shaft, a first stepper motor is started to drive the first rotating shaft to rotate, the rotation of the first rotating shaft drives the disc and the first circular plate to rotate, and the rotation of the disc and the first circular plate drives the test tube to revolve around the first rotating shaft, so as to preliminarily mix the bacterial suspension and saline in the test tube. Then, through the arrangement of a sleeve, a first gear, and a second gear, the rotation of the disc drives the sleeve to revolve around the first rotating shaft, the outer side of the second gear is meshed with the outer side of the first gear, and the fixed ring and the second gear are fixed, so that the sleeve and the test tube rotate, and the rotation of the test tube cooperates with the revolution to improve the efficiency of mixing the bacterial suspension and the saline, which is conducive to improving the efficiency of preparing the bacterial suspension.
[0021] The present invention discloses a bacterial suspension preparation instrument for medical clinical microorganism detection. Through the arrangement of a push rod, a push rod, an arc plate, an arc rubber piece, a first rubber ring and a second rubber ring, the rotation of a disc drives the fixed tube and the push rod to rotate, and the lower end of the push rod moves from the first plane part of the annular guide block to the second plane part of the annular guide block through the inclined part of the annular guide block, so that the push rod moves upward under the guiding action of the inclined part of the annular guide block; the upper end of the push rod is in sliding contact with the inclined surface of one end of the push rod, so that the push rod moves upward to push the push rod to move inward, and the movement of the push rod pushes the arc plate and the arc rubber piece to move; and under the action of the first rubber ring and the second rubber ring, the arc rubber piece pushes the upper part of the test tube to move, so that the test tube is tiltedly arranged in the first rubber ring and the second rubber ring; so that in the process of the lower end of the push rod moving on the second plane part of the annular guide block, the test tube is in a tilted arrangement state, and the self-rotation and revolution of the test tube cooperate to further improve the efficiency of uniform mixing of the bacterial suspension and the physiological saline. The movement of the push rod drives the movement of the slider through the setting of the slider, the spring and the groove, and the movement of the slider squeezes the spring to generate elastic force; a wave-like uneven structure is formed through a plurality of grooves on one side of the second plane portion of the annular guide block, and under the elastic force of the spring, the push rod moves back and forth, and the back and forth movement of the push rod drives the arc plate and the arc rubber part to move back and forth, and the back and forth movement of the arc rubber part pushes the test tube to swing back and forth, so that the bacterial suspension in the test tube is fully and evenly mixed with the physiological saline, which is beneficial to improving the efficiency of bacterial suspension preparation. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.
[0023] The present invention is further described below in conjunction with the accompanying drawings and embodiments.
[0024] Figure 1 It is an isometric structural schematic diagram of the present invention;
[0025] Figure 2 It is a front view structural schematic diagram of the present invention;
[0026] Figure 3 It is a schematic diagram of the top view structure of the present invention;
[0027] Figure 4 for Figure 3 Schematic diagram of the cross-sectional structure at AA in the middle;
[0028] Figure 5 for Figure 4 A schematic diagram of the structure of the partial enlarged view at C in the middle;
[0029] Figure 6 for Figure 4 A schematic diagram of the structure of the local enlarged view at D in the middle;
[0030] Figure 7 for Figure 4 The structural schematic diagram of the local enlarged view at E in the middle;
[0031] Figure 8 for Figure 7 The structural schematic diagram of the local enlarged view at F in the middle;
[0032] Fig. 9 for Figure 4 Schematic diagram of the cross-sectional structure at the middle BB;
[0033] Fig.10 for Fig. 9 A schematic diagram of the structure of the local enlarged view at G in the middle;
[0034] Fig.11 It is an isometric structural schematic diagram of the internal structure of the shell in the present invention.
[0035] Description of reference numerals:
[0036] Shell 10, control button 11, display screen 12, first closed door 13, first fixing plate 14, second fixing plate 15, partition 16, sampling chamber 17, preparation chamber 18, operation chamber 19, second closed door 20, third closed door 21, first mounting plate 22, disc 23, first circular plate 24, sleeve 25, first rubber ring 26, first annular plate 27, second rubber ring 28, fixing tube 29, ejector rod 30, push rod 31, slider 32, spring 33, arc plate 34, arc rubber member 35, test tube 36, annular guide block 37, annular elastic member 38, groove 3 9. First rotating shaft 40, first stepper motor 41, first gear 42, fixed ring 43, second gear 44, physiological saline tank 45, first feed tube 46, turbidimeter 47, second mounting plate 48, second circular plate 49, second stepper motor 50, limit shell 51, solution tank 52, electric telescopic rod 53, third stepper motor 54, second rotating shaft 55, third circular plate 56, movable plate 57, pipette 58, second feed tube 59, fixed block 60, telescopic rod 61, camera 62, placement hole 63, sterilization ultraviolet light lamp 64, fan 65, second annular plate 66. DETAILED DESCRIPTION
[0037] The following embodiments of the present invention are described in further detail in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.
[0038] In the description of the present invention, unless otherwise specified, "plurality" means two or more than two; the orientations or positional relationships indicated by the terms "upper", "lower", "left", "right", "inner", "outer", "front end", "rear end", "head", "tail", etc. are based on the orientations or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", "third", etc. are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.
[0039] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "connected" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.
[0040] As attached Figure 1 To Attachment Fig.11 As shown:
[0041] The invention provides a bacterial suspension preparation instrument for medical clinical microbiological detection.
[0042] See attached Figure 1 To Attachment Fig.11 , comprising a shell 10, a first fixing plate 14 is fixedly provided on the lower side of the interior of the shell 10, a second fixing plate 15 is fixedly provided on the upper side of the interior of the shell 10, a partition plate 16 is fixedly connected between the first fixing plate 14 and the second fixing plate 15, a sampling chamber 17 and a preparation chamber 18 are separated in the middle of the shell 10 by the first fixing plate 14, the second fixing plate 15 and the partition plate 16, an operation chamber 19 is separated in the upper part of the shell 10 by the second fixing plate 15, a sampling assembly is provided in the operation chamber 19, and a preparation assembly is provided in the preparation chamber 18; the preparation assembly comprises a first mounting plate 22, the first mounting plate 22 is fixed on the lower side of the interior of the preparation chamber 18, and a rotatable mounting plate 22 is provided on the upper side of the first mounting plate 22 A disc 23 is provided, in the middle of which a first rotating shaft 40 is fixedly provided, and at the upper end of which a first circular plate 24 is fixedly provided, a plurality of sleeves 25 are provided in a circumferential array on the disc 23, and a first rubber ring 26 is provided on the inner side of each sleeve 25, and a plurality of second rubber rings 28 are provided in a circumferential array on the first circular plate 24, and a plurality of test tubes 36 are placed in a circumferential array on the disc 23 and the first circular plate 24, and a plurality of fixed tubes 29 are connected between the upper side of the disc 23 and the lower side of the first circular plate 24, and a push rod 31 is slidably provided on one side of each fixed tube 29, and an arc plate 34 is fixedly provided on one end of each push rod 31 close to the test tube 36, and an arc-shaped rubber member 35 is provided on the inner side of each arc plate 34.
[0043] Preferably, see Attachment Figure 7 To Attachment Fig.10 The inner side of each first rubber ring 26 is in extrusion contact with the outer side of the lower part of the test tube 36, the inner side of each second rubber ring 28 is in extrusion contact with the outer side of the upper part of the test tube 36, the inner side of each arc-shaped rubber member 35 is in contact with the outer side of the middle part of the test tube 36, and a push rod 30 is vertically slidably provided inside each fixed tube 29, and the upper end of each push rod 30 is in sliding contact with the inclined surface of one end of the push rod 31.
[0044] Preferably, see Attachment Figure 7 To Attachment Fig.10 A slider 32 is fixedly provided on one side of each push rod 31, and a spring 33 is connected between each slider 32 and the inner wall of the fixed tube 29. An annular guide block 37 is fixedly provided on the upper side of the first mounting plate 22. The upper part of the annular guide block 37 includes a first plane portion, a second plane portion, and two inclined portions. The first plane portion of the annular guide block 37 is lower than the second plane portion of the annular guide block 37. The two ends of the first plane portion of the annular guide block 37 are respectively connected to the two ends of the second plane portion of the annular guide block 37 through the two inclined portions of the annular guide block 37. The lower end of each push rod 30 is in sliding contact with the upper part of the annular guide block 37.
[0045] Preferably, see Attachment Fig. 9 To Attachment Fig.10 A plurality of grooves 39 are provided at intervals on one side of the second plane portion of the annular guide block 37. A plurality of grooves 39 are used to form a wavy uneven structure on one side of the second plane portion of the annular guide block 37. An annular elastic member 38 is provided on the upper side of the first mounting plate 22. The lower end of each test tube 36 is in sliding contact with the upper side of the annular elastic member 38.
[0046] Preferably, see Attachment Figure 7 To Attachment Fig.10 A first annular plate 27 is fixedly provided on the outer side of the middle part of each sleeve 25, and each first annular plate 27 slides in annular shape on the upper part of the disc 23. A first gear 42 is fixedly provided on the outer side of the lower part of each sleeve 25. A fixing ring 43 is fixedly provided on the upper side of the first mounting plate 22, and a second gear 44 is fixedly provided on the upper end of the fixing ring 43. The outer side of each first gear 42 is meshed with the outer side of the second gear 44. A second annular plate 66 is fixedly provided on the outer side of each second rubber ring 28, and each second annular plate 66 slides in annular shape inside the first disc 24. The outer side of each second rubber ring 28 is in sliding contact with the inner side of the first disc 24, and the outer side of each sleeve 25 is in sliding contact with the upper part of the disc 23.
[0047] Preferably, see Attachment Figure 7 To Attachment Fig. 9 A physiological saline tank 45 is provided on the upper side of the preparation chamber 18, a first discharge pipe 46 is provided at the lower end of the physiological saline tank 45, a turbidimeter 47 is installed on the lower side of the preparation chamber 18, a first stepper motor 41 is installed at the lower end of the first mounting plate 22, and the output end of the first stepper motor 41 is fixedly connected to the lower end of the first rotating shaft 40.
[0048] Preferably, see Attachment Figure 4 To Attachment Figure 5 A second mounting plate 48 is fixedly provided inside the sampling chamber 17, a second circular plate 49 is rotatably provided on the upper side of the second mounting plate 48, a plurality of limit shells 51 are provided in a circular array on the upper part of the second circular plate 49, a fixed block 60 is fixedly provided on the outer side of each limit shell 51, a telescopic rod 61 is fixedly provided on the upper side of the second mounting plate 48, the telescopic rod 61 is located on the outer side of the second circular plate 49, the protruding end of the telescopic rod 61 contacts the lower side of the fixed block 60, a second stepping motor 50 is installed on the lower side of the second mounting plate 48, and the output end of the second stepping motor 50 is fixedly connected to the second circular plate 49.
[0049] Preferably, see Attachment Figure 4 To Attachment Figure 5 A solution box 52 is provided on one side of the upper portion of the sampling chamber 17 , a second feed pipe 59 is provided on the lower side of the solution box 52 , and a camera 62 is installed on the upper portion of the sampling chamber 17 .
[0050] Preferably, see Attachment Figure 1 To Attachment Figure 4 A first closed door 13 is installed on the upper side of the operating chamber 19, a second closed door 20 is installed on one side of the sampling chamber 17, a third closed door 21 is installed on one side of the preparation chamber 18, a sterilization ultraviolet light lamp 64 is installed on one side of the upper part of the operating chamber 19, a fan 65 is provided on the other side of the upper part of the operating chamber 19, a plurality of control buttons 11 are provided on one side of the lower part of the shell 10, and a display screen 12 is installed on one side of the upper part of the shell 10.
[0051] Preferably, see Attachment Figure 4 , Attachment Figure 6 The sampling assembly includes an electric telescopic rod 53, which is fixed on the upper side of the operating chamber 19. A movable plate 57 is fixedly provided at the protruding end of the electric telescopic rod 53, and a third stepper motor 54 is installed on the movable plate 57. A second rotating shaft 55 is fixedly provided at the output end of the third stepper motor 54, and a third circular plate 56 is fixedly provided at the lower end of the second rotating shaft 55. A plurality of placement holes 63 are provided in a circular array on the third circular plate 56, and a pipette 58 is placed inside each placement hole 63. The upper part of the pipette 58 is limited on the upper side of the third circular plate 56.
[0052] Specific use of the present invention:
[0053] First, the staff rotates the first closed door 13 to open the operation chamber 19, takes out the six pipettes 58 on the third circular plate 56 to replace the gun tips, puts the six pipettes 58 with replaced gun tips back into the six placement holes 63 of the third circular plate 56, and marks the six pipettes 58 with numbers 1, 2, 3, 4, 5, and 6 respectively through the control system. The staff rotates the first closed door 13 to close the operation chamber 19. Next, the staff rotates the third closed door 21 to open the preparation chamber 18, puts the six test tubes 36 between the disc 23 and the first circular plate 24, and squeezes and limits the outer sides of the lower and upper parts of the test tubes 36 through the first rubber ring 26 and the second rubber ring 28, respectively, so that the lower end of the test tube 36 is located on the upper side of the annular elastic member 38. The control system marks the six test tubes 36 with numbers 1, 2, 3, 4, 5, and 6 respectively. The staff member turns the third closed door 21 to close the preparation chamber 18. Then the control system controls the first stepper motor 41 to start. The first stepper motor 41 starts to drive the first rotating shaft 40 to rotate. The first rotating shaft 40 rotates to drive the disc 23 and the first circular plate 24 to rotate, so that the six test tubes 36 are moved to the bottom of the saline tank 45 in sequence. The control system controls the first discharge pipe 46 to open, so that the saline in the saline tank 45 enters the six test tubes 36 in sequence, so that the six test tubes 36 are filled with 9 ml of saline. Among them, the No. 1 pipette 58 is located directly above one of the limit shells 51, and the No. 4 pipette 58 is located in the gap between the No. 1 test tube 36 and the No. 2 test tube 36.
[0054] Secondly, the staff rotates the second closed door 20 to open the sampling chamber 17, and puts the purified flat plate into the limiting shell 51. At this time, the control system controls the second feed pipe 59 to open, so that the liquid culture medium in the solution box 52 falls into the flat plate in the limiting shell 51 through the second feed pipe 59. The second feed pipe 59 is closed, and the control system controls the second stepper motor 50 to start. The second stepper motor 50 starts to drive the second circular plate 49 to rotate. The rotation of the second circular plate 49 drives the limiting shell 51 and the flat plate to move to the bottom of the No. 1 pipette 58. At this time, the fixed block 60 is located above the telescopic rod 61, and the control system controls the telescopic rod 61 to extend. The extension of the telescopic rod 61 pushes the fixed block 60 to move upward. The upward movement of the fixed block 60 lifts one side of the limiting shell 51 upward, so that the limiting shell 51 and the flat plate are tilted and placed on the upper part of the second circular plate 49.
[0055] Then, the control system controls the electric telescopic rod 53 to extend, and the electric telescopic rod 53 extends to drive the movable plate 57 and the second rotating shaft 55 to move downward, and the second rotating shaft 55 moves downward to drive the third circular plate 56 to move downward, and the third circular plate 56 moves downward to make the tip of the No. 1 pipette 58 contact the flat plate. Then, the third stepper motor 54 starts to drive the second rotating shaft 55 to rotate forward and reverse in a small range alternately, and the second rotating shaft 55 rotates to drive the third circular plate 56 and the tip of the No. 1 pipette 58 to move back and forth in a small range, and the tip of the No. 1 pipette 58 moves back and forth to scrape the blocky bacterial strains on the surface of the flat plate. The control system controls the No. 1 pipette 58 to repeatedly absorb the solution in the flat plate, and spray the solution into the flat plate again, so as to break up the clumped bacterial strains by the solution. The camera 62 is used to detect the breaking up of the bacterial strains on the surface of the flat plate.
[0056] After the clumped bacteria in the plate are broken up, the control system controls the No. 1 pipette 58 to start sucking 1 ml of the solution in the plate into the gun tip. The control system controls the electric telescopic rod 53 to contract and drive the third circular plate 56 to move upward, so that the gun tip of the No. 1 pipette 58 is moved out of the plate. Then, the third stepper motor 54 starts to drive the second rotating shaft 55 to rotate, the second rotating shaft 55 rotates to drive the third circular plate 56 to rotate, and the third circular plate 56 rotates to drive the gun tip of the No. 1 pipette 58 to move directly above the No. 1 test tube 36. The control system controls the No. 1 pipette 58 to spray 1 ml of the solution in the gun tip into the No. 1 test tube 36. At this time, the solution in the No. 1 test tube 36 is 10 ml of the shell.
[0057] Next, the control system controls the first stepper motor 41 to start, and the first stepper motor 41 starts to drive the first rotating shaft 40 to rotate, and the first rotating shaft 40 rotates to drive the disc 23 and the first circular plate 24 to rotate, and the disc 23 and the first circular plate 24 rotate to drive the No. 1 test tube 36 to revolve around the first rotating shaft 40, so as to preliminarily mix the bacterial suspension and the physiological saline in the No. 1 test tube 36. The disc 23 rotates to drive the sleeve 25 to revolve around the first rotating shaft 40, and the outer side of the second gear 44 is meshed with the outer side of the first gear 42, and the fixing ring 43 and the second gear 44 are fixed, so that the sleeve 25 and the No. 1 test tube 36 rotate, and the rotation and revolution of the No. 1 test tube 36 cooperate to improve the efficiency of mixing the bacterial suspension and the physiological saline, which is conducive to improving the efficiency of preparing the bacterial suspension. The rotation of the sleeve 25 drives the first annular plate 27 to slide in an annular manner in the upper part of the disc 23, and the rotation of the test tube 36 drives the second rubber ring 28 and the second annular plate 66 to rotate, so that the second annular plate 66 slides in an annular manner in the first circular plate 24, so as to make the test tube 36 rotate smoothly.
[0058] At the same time, the rotation of the disk 23 drives the fixed tube 29 and the push rod 30 to rotate, and the lower end of the push rod 30 moves from the first plane portion of the annular guide block 37 to the second plane portion of the annular guide block 37 through the inclined portion of the annular guide block 37, so that the push rod 30 moves upward under the guidance of the inclined portion of the annular guide block 37. The upper end of the push rod 30 slides in contact with the inclined surface of one end of the push rod 31, so that the push rod 30 moves upward to push the push rod 31 to move radially inward, and the push rod 31 moves to push the arc plate 34 and the arc rubber member 35. And under the action of the first rubber ring 26 and the second rubber ring 28, the arc rubber member 35 pushes the upper part of the No. 1 test tube 36 to move, so that the No. 1 test tube 36 is arranged obliquely in the first rubber ring 26 and the second rubber ring 28. Thus, during the movement of the lower end of the push rod 30 on the second plane portion of the annular guide block 37, the No. 1 test tube 36 is in an inclined arrangement state, and the rotation and revolution of the No. 1 test tube 36 cooperate, which is conducive to further improving the efficiency of uniform mixing of the bacterial suspension and the physiological saline. Among them, the movement of the push rod 31 drives the slider 32 to move, and the movement of the slider 32 squeezes the spring 33 to generate elastic force. A wave-shaped uneven structure is formed by a plurality of grooves 39 on one side of the second plane portion of the annular guide block 37, and under the elastic force of the spring 33, the push rod 31 moves back and forth, and the push rod 31 moves back and forth to drive the arc plate 34 and the arc rubber member 35 to move back and forth, and the arc rubber member 35 moves back and forth to swing the No. 1 test tube 36 back and forth, so that the bacterial suspension in the No. 1 test tube 36 is fully mixed with the physiological saline, which is conducive to improving the efficiency of bacterial suspension preparation.
[0059] When the solution in test tube No. 1 36 is mixed evenly, the disc 23 rotates to drive test tube No. 1 36 to move to the top of the turbidimeter 47 , and the turbidimeter 47 measures the light transmission or scattering of the solution in test tube No. 1 36 to estimate the number of bacteria, so as to quickly detect the concentration of the bacterial suspension in test tube No. 1 36 .
[0060] When the turbidimeter 47 detects that the concentration of the bacterial suspension in the No. 1 test tube 36 does not meet the requirements, the concentration of the bacterial suspension in the No. 1 test tube 36 is adjusted in time by adding physiological saline through the physiological saline tank 45 or by sucking the solution in the plate into the No. 1 test tube 36 through the No. 1 pipette 58.
[0061] When the turbidimeter 47 detects that the concentration of the bacterial suspension in the No. 1 test tube 36 meets the requirements, at this time, the No. 1 test tube 36 contains a 10% dilution of the shell bacterial suspension. The No. 1 test tube 36 moves to the bottom of the No. 2 pipette 58, and the control system controls the electric telescopic rod 53 to extend, and the electric telescopic rod 53 extends to drive the No. 2 pipette 58 to move downward, so that the tip of the No. 2 pipette 58 enters the No. 1 test tube 36, and the start of the No. 2 pipette 58 absorbs 1 ml of the solution in the No. 1 test tube 36 into the tip of the No. 2 pipette 58. The first stepper motor 41 starts to drive the disc 23 and the first circular plate 24 to rotate, so that the No. 2 test tube 36 moves to the bottom of the No. 2 pipette 58, and the control system controls the No. 2 pipette 58 to spray, and sprays the 1 ml dilution of the bacterial suspension in the tip of the No. 2 pipette 58 into the No. 2 test tube 36. The preparation component starts to shake the No. 2 test tube 36 vigorously, which is conducive to fully mixing the bacterial suspension in the No. 2 test tube 36 with the physiological saline. According to the above steps, the six test tubes 36 are diluted with bacterial suspension in turn, so that the contents of the No. 1, 2, 3, 4, 5, and 6 test tubes 36 correspond to the shell 10. -1 To housing 10 -6 dilution of bacterial suspension.
[0062] Finally, the working solution controls the control system through a plurality of control buttons 11, so that a bacterial suspension of a specific concentration can be quickly prepared as needed to improve the efficiency of microbial detection. The fan 65 is started to ventilate the interior of the housing 10, and the sterilizing ultraviolet light lamp 64 is started to sterilize and disinfect the interior of the housing 10.
[0063] The present invention provides a bacterial suspension preparation instrument for medical clinical microorganism detection. Through the arrangement of the disc 23, the first circular plate 24, and the first rotating shaft 40, the first stepper motor 41 is started to drive the first rotating shaft 40 to rotate. The rotation of the first rotating shaft 40 drives the disc 23 and the first circular plate 24 to rotate. The rotation of the disc 23 and the first circular plate 24 drives the No. 1 test tube 36 to revolve around the first rotating shaft 40, so as to preliminarily mix the bacterial suspension in the No. 1 test tube 36 with the physiological saline. Then, through the arrangement of the sleeve 25, the first gear 42, and the second gear 44, the disc 23 rotates to drive the sleeve 25 to revolve around the first rotating shaft 40, and the outer side of the second gear 44 is meshed with the outer side of the first gear 42, and the fixing ring 43 and the second gear 44 are fixed, so that the sleeve 25 and the No. 1 test tube 36 rotate, and the rotation and revolution of the No. 1 test tube 36 cooperate to improve the efficiency of mixing the bacterial suspension and the physiological saline, which is conducive to improving the efficiency of bacterial suspension preparation.
[0064] The present invention provides a bacterial suspension preparation instrument for medical clinical microbial detection. Through the arrangement of a push rod 30, a push rod 31, an arc plate 34, an arc rubber member 35, a first rubber ring 26, and a second rubber ring 28, the rotation of the disk 23 drives the fixed tube 29 and the push rod 30 to rotate. The lower end of the push rod 30 moves from the first plane portion of the annular guide block 37 through the inclined portion of the annular guide block 37 to the second plane portion of the annular guide block 37, so that the push rod 30 moves upward under the guidance of the inclined portion of the annular guide block 37; the upper end of the push rod 30 slides in contact with the inclined surface of one end of the push rod 31, so that the push rod The push rod 30 moves upward to push the push rod 31 to move radially inward, and the push rod 31 moves to push the arc plate 34 and the arc rubber member 35; and under the action of the first rubber ring 26 and the second rubber ring 28, the arc rubber member 35 pushes the upper part of the No. 1 test tube 36 to move, so that the No. 1 test tube 36 is arranged obliquely in the first rubber ring 26 and the second rubber ring 28; therefore, during the movement of the lower end of the push rod 30 on the second plane portion of the annular guide block 37, the No. 1 test tube 36 is arranged obliquely, and the rotation and revolution of the No. 1 test tube 36 cooperate to further improve the efficiency of mixing the bacterial suspension and the physiological saline. The push rod 31 is moved by the setting of the slider 32, the spring 33 and the groove 39, and the slider 32 is squeezed by the movement of the slider 32 to generate elastic force; a wave-like uneven structure is formed by a plurality of grooves 39 on one side of the second plane portion of the annular guide block 37, and under the elastic force of the spring 33, the push rod 31 moves back and forth, and the push rod 31 moves back and forth to drive the arc plate 34 and the arc rubber member 35 to move back and forth, and the arc rubber member 35 moves back and forth to swing the No. 1 test tube 36 back and forth, so that the bacterial suspension in the No. 1 test tube 36 is fully mixed with the physiological saline, which is beneficial to improving the efficiency of bacterial suspension preparation.
[0065] The embodiments of the present invention are given for the purpose of illustration and description, and are not intended to be exhaustive or to limit the invention to the disclosed forms. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiments are selected and described in order to better illustrate the principles and practical applications of the present invention and to enable those of ordinary skill in the art to understand the present invention and thereby design various embodiments with various modifications suitable for specific uses.
Claims
1. A bacterial suspension preparation instrument for medical clinical microbiological detection, characterized in that: The invention comprises a shell (10), wherein a first fixing plate (14) is fixedly provided on the lower inner side of the shell (10), a second fixing plate (15) is fixedly provided on the upper inner side of the shell (10), a partition plate (16) is fixedly provided between the first fixing plate (14) and the second fixing plate (15), a sampling chamber (17) and a preparation chamber (18) are separated in the middle of the shell (10) by the first fixing plate (14), the second fixing plate (15) and the partition plate (16), an operation chamber (19) is separated in the upper part of the shell (10) by the second fixing plate (15), a sampling assembly is provided in the operation chamber (19), and a preparation assembly is provided in the preparation chamber (18); the preparation assembly comprises a first mounting plate (22), the first mounting plate (22) is fixedly provided on the lower inner side of the preparation chamber (18), and a circular rotating member is provided on the upper side of the first mounting plate (22) so as to rotate. A disk (23), wherein a first rotating shaft (40) is fixedly arranged in the middle of the disk (23), a first circular plate (24) is fixedly arranged at the upper end of the first rotating shaft (40), a plurality of sleeves (25) are arranged in a circular array on the disk (23), a first rubber ring (26) is arranged on the inner side of each sleeve (25), a plurality of second rubber rings (28) are arranged in a circular array on the first circular plate (24), a plurality of test tubes (36) are placed in a circular array on the disk (23) and the first circular plate (24), a plurality of fixed tubes (29) are connected between the upper side of the disk (23) and the lower side of the first circular plate (24), a push rod (31) is slidably arranged on one side of each fixed tube (29), an arc plate (34) is fixedly arranged on one end of each push rod (31) close to the test tube (36), and an arc rubber piece (35) is arranged on the inner side of each arc plate (34); The inner side of each first rubber ring (26) is in extrusion contact with the outer side of the lower part of the test tube (36), the inner side of each second rubber ring (28) is in extrusion contact with the outer side of the upper part of the test tube (36), the inner side of each arc-shaped rubber member (35) is in contact with the outer side of the middle part of the test tube (36), and a push rod (30) is vertically slidably provided inside each fixed tube (29), and the upper end of each push rod (30) is in sliding contact with the inclined surface of one end of the push rod (31); A slider (32) is fixedly provided on one side of each push rod (31), a spring (33) is connected between each slider (32) and the inner wall of the fixed tube (29), an annular guide block (37) is fixedly provided on the upper side of the first mounting plate (22), the upper part of the annular guide block (37) comprises a first plane part, a second plane part and two inclined parts, the first plane part of the annular guide block (37) is lower than the second plane part of the annular guide block (37), the two ends of the first plane part of the annular guide block (37) are respectively connected to the two ends of the second plane part of the annular guide block (37) through the two inclined parts of the annular guide block (37), and the lower end of each push rod (30) is in sliding contact with the upper part of the annular guide block (37); A plurality of grooves (39) are arranged at intervals on one side of the second plane portion of the annular guide block (37); a wave-shaped uneven structure is formed on one side of the second plane portion of the annular guide block (37) through the plurality of grooves (39); an annular elastic member (38) is arranged on the upper side of the first mounting plate (22); and the lower end of each test tube (36) is in sliding contact with the upper side of the annular elastic member (38).
2. The bacterial suspension preparation instrument for medical clinical microbiological detection according to claim 1, characterized in that: A first annular plate (27) is fixedly provided on the outer side of the middle part of each sleeve (25), and each first annular plate (27) slides in annular shape on the upper part of the disc (23). A first gear (42) is fixedly provided on the outer side of the lower part of each sleeve (25). A fixing ring (43) is fixedly provided on the upper side of the first mounting plate (22), and a second gear (44) is fixedly provided on the upper end of the fixing ring (43). The outer side of each first gear (42) meshes with the outer side of the second gear (44). A second annular plate (66) is fixedly provided on the outer side of each second rubber ring (28), and each second annular plate (66) slides in annular shape inside the first disc (24). The outer side of each second rubber ring (28) is in sliding contact with the inner side of the first disc (24), and the outer side of each sleeve (25) is in sliding contact with the upper part of the disc (23).
3. The bacterial suspension preparation instrument for medical clinical microbiological detection according to claim 1, characterized in that: A physiological saline tank (45) is provided on one side of the upper part of the preparation chamber (18), a first discharge pipe (46) is provided at the lower end of the physiological saline tank (45), a turbidity meter (47) is installed on one side of the lower part of the preparation chamber (18), a first stepper motor (41) is installed at the lower end of the first mounting plate (22), and an output end of the first stepper motor (41) is fixedly connected to the lower end of the first rotating shaft (40).
4. The bacterial suspension preparation instrument for medical clinical microbiological detection according to claim 1, characterized in that: A second mounting plate (48) is fixedly provided inside the sampling chamber (17); a second circular plate (49) is rotatably provided on the upper side of the second mounting plate (48); a plurality of limiting shells (51) are provided in a circular array on the upper part of the second circular plate (49); a fixing block (60) is fixedly provided on the outer side of each limiting shell (51); a telescopic rod (61) is fixedly provided on the upper side of the second mounting plate (48); the telescopic rod (61) is located on the outer side of the second circular plate (49); the protruding end of the telescopic rod (61) contacts the lower side of the fixing block (60); a second stepping motor (50) is installed on the lower side of the second mounting plate (48); and the output end of the second stepping motor (50) is fixedly connected to the second circular plate (49).
5. The bacterial suspension preparation instrument for medical clinical microbiological detection according to claim 4, characterized in that: A solution box (52) is provided on one side of the upper portion of the sampling cavity (17), a second feed pipe (59) is provided on the lower side of the solution box (52), and a camera (62) is installed on the upper portion of the sampling cavity (17).
6. The bacterial suspension preparation instrument for medical clinical microbiological detection according to claim 1, characterized in that: A first closed door (13) is installed on the upper side of the operating chamber (19), a second closed door (20) is installed on one side of the sampling chamber (17), a third closed door (21) is installed on one side of the preparation chamber (18), a sterilizing ultraviolet light lamp (64) is installed on one side of the upper part of the operating chamber (19), a fan (65) is provided on the other side of the upper part of the operating chamber (19), a plurality of control buttons (11) are provided on one side of the lower part of the shell (10), and a display screen (12) is installed on one side of the upper part of the shell (10).
7. The bacterial suspension preparation instrument for medical clinical microbiological detection according to claim 1, characterized in that: The sampling assembly comprises an electric telescopic rod (53), wherein the electric telescopic rod (53) is fixed on the upper side of the operating chamber (19), a movable plate (57) is fixedly provided at the protruding end of the electric telescopic rod (53), a third stepping motor (54) is mounted on the movable plate (57), a second rotating shaft (55) is fixedly provided at the output end of the third stepping motor (54), a third circular plate (56) is fixedly provided at the lower end of the second rotating shaft (55), a plurality of placement holes (63) are arranged in a circular array on the third circular plate (56), a pipette (58) is placed inside each of the placement holes (63), and the upper part of the pipette (58) is limited on the upper side of the third circular plate (56).
Citation Information
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