Thin-layer identification method of medicinal preparation for dispersing depression and stimulating menstrual flow

Abstract

The invention belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a thin-layer identification method of a depression-dispersing and menstruation-stimulating medicinal preparation. The invention provides a thin-layer identification method of a depression-dispersing and menstruation-stimulating medicinal preparation, which comprises the following steps: preparing a test solution A, and simultaneously identifying cape jasmine, liquorice, white paeony root and moutan bark in the depression-dispersing and menstruation-stimulating medicinal preparation by adopting a thin-layer chromatography based on the test solution A, according to thin-layer chromatography, the test solution A, the reference medicinal material solution and the reference substance solution are taken and applied to the same silica gel thin-layer plate respectively, and developing is conducted with ethyl acetate, formic acid, glacial acetic acid and water with the volume ratio being 15: (1-3): (1-3): 2 as a developing solvent. The thin-layer identification method can be used for simultaneously detecting liquorice, cape jasmine, white paeony root and moutan bark in the depression-dispersing and menstruation-stimulating medicinal preparation, and is short in detection time and high in efficiency.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine detection, and in particular relates to a thin layer identification method for a depression-relieving and menstruation-promoting drug preparation. Background Art

[0002] Xuanyu Tongjing Decoction comes from Fu Shan's "Fu Qingzhu Women's Medicine" in the Qing Dynasty. The original text records: "White Peony Root 5 qian (fried with wine), Angelica 5 qian (washed with wine), Cortex Moutan 5 qian, Gardenia 3 qian (fried), White Mustard Seed 2 qian (fried and ground), Bupleurum 1 qian, Cyperus rotundus 1 qian (fried with wine), Curcuma 1 qian (fried with vinegar), Scutellaria 1 qian (fried with wine), Licorice 1 qian". This prescription has the characteristics of nourishing liver blood, relieving liver depression, benefiting liver qi, and reducing liver fire. In the prescription, white peony nourishes blood, regulates menstruation, soothes the liver and relieves pain; Cortex Moutan clears heat and cools blood, promotes blood circulation and relieves blood stasis; Gardenia purges fire and relieves restlessness, clears heat and promotes dampness, cools blood and relieves pain; Licorice clears heat and removes phlegm, relieves urgency and relieves pain, and harmonizes the properties of the medicine; the whole prescription promotes the circulation of qi and blood, and eliminates dampness and phlegm.

[0003] As a classic gynecological prescription, Xuanyu Tongjing Decoction has great development value and needs to be tested for its medicinal properties. Thin layer chromatography is an important qualitative detection method in the Chinese Pharmacopoeia, but common thin layer chromatography detection is to detect each medicinal property separately, that is, one thin layer plate is used to identify one prescription medicinal property. This method is cumbersome and has low detection efficiency. In addition, there is no thin layer identification method for the multiple components of Xuanyu Tongjing Decoction in the prior art. Summary of the invention

[0004] Therefore, the technical problem to be solved by the present invention is to overcome the defects in the prior art that multiple ingredients in Xuanyu Tongjing Decoction cannot be detected simultaneously, resulting in complicated steps and low detection efficiency, thereby providing a thin layer identification method for Xuanyu Tongjing pharmaceutical preparations, which can simultaneously detect the ingredients of licorice, gardenia, white peony root and peony bark in Xuanyu Tongjing pharmaceutical preparations, with short detection time and high efficiency.

[0005] To this end, the present invention provides the following technical solutions.

[0006] The present invention provides a thin layer identification method of a drug preparation for relieving depression and promoting menstruation, comprising the following steps:

[0007] A test solution A is prepared, and based on the test solution A, a thin layer chromatography method is used to simultaneously identify Gardenia, Licorice, White Peony Root and Paeonia Suffruticosa Root in the Xuanyu Tongjing drug preparation;

[0008] According to thin layer chromatography:

[0009] The test solution A, gardenia control medicinal material solution, licorice control medicinal material solution, peony bark control medicinal material solution, white peony control medicinal material solution, gardenia glycoside reference solution, liquiritin reference solution, and paeoniflorin reference solution were spotted on the same silica gel thin layer plate, and developed with ethyl acetate, formic acid, glacial acetic acid and water in a volume ratio of 15:(1-3):(1-3):2 as the developing solvent.

[0010] Furthermore, in the developing solvent, the volume ratio of ethyl acetate, formic acid, glacial acetic acid and water is 15:1:1:2.

[0011] Optionally, the preparation process of the test solution A comprises: taking a test sample of the Xuanyutongjing drug preparation, adding methanol to dissolve it, and then separating the solid and liquid, taking the liquid and evaporating it to dryness, adding water to dissolve the obtained residue and then separating the solid and liquid, taking the liquid and adding water-saturated n-butanol to extract, taking the liquid and washing it with water saturated with n-butanol, taking the n-butanol washing liquid and evaporating it to dryness, and adding methanol to re-dissolve the obtained residue;

[0012] Exemplarily, 5-10 g of a test sample of the Xuanyutongjing drug preparation is taken, 10-50 ml of methanol is added to dissolve it, and then the solid and liquid are separated. The liquid is evaporated to dryness, 20-40 ml of water is added to the residue to dissolve it, and then the solid and liquid are separated. The liquid is extracted with water-saturated n-butanol for 1-3 times, 20-50 ml of volume each time, the extracts are combined, the liquid is washed with water saturated with n-butanol for 1-3 times, 20-50 ml of volume each time, the n-butanol washing liquid is evaporated to dryness, and the residue is redissolved with 1-3 ml of methanol; the ratio of weight to volume is g / ml.

[0013] Optionally, the method further comprises the steps of preparing the Gardenia control medicinal material solution, the Licorice control medicinal material solution, the Moutan Cortex control medicinal material solution and the White Peony Root control medicinal material solution according to the preparation process of the test solution A;

[0014] Exemplarily, 0.5 to 2 weight parts of each of Gardenia jasminoides, Licorice root, Moutan bark and White Peony root are taken, 10 to 50 ml of methanol are added to each of them for dissolution, followed by solid-liquid separation, the liquid is evaporated to dryness, 20 to 40 ml of water is added to the obtained residue for dissolution, followed by solid-liquid separation, the liquid is extracted with water-saturated n-butanol for 1 to 3 times, 20 to 50 ml of volume each time, the extracts are combined, the liquid is washed with water saturated with n-butanol for 1 to 3 times, 20 to 50 ml of volume each time, the n-butanol washing liquid is evaporated to dryness, and 1 to 3 ml of methanol is added to the obtained residue for redissolution to prepare the corresponding control medicinal material solutions; the ratio of weight parts to volume parts is g / ml.

[0015] Exemplarily, the paeoniflorin reference substance solution is prepared by dissolving the paeoniflorin reference substance in methanol, wherein 1-5 mg of the paeoniflorin reference substance is dissolved in every 1 mL of methanol;

[0016] Exemplarily, the gardenoside reference solution is prepared by dissolving the gardenoside reference in methanol, wherein 1-5 mg of the gardenoside reference is dissolved in every 1 mL of methanol;

[0017] Illustratively, the liquiritin reference solution is prepared by dissolving the liquiritin reference in methanol, with 1-5 mg of the liquiritin reference dissolved in every 1 mL of methanol.

[0018] Optionally, the spotting volume of the test solution A is 3-8 μL;

[0019] Optionally, the spotting volume of the Gardenia jasminoides control medicinal material solution, the Licorice control medicinal material solution, the Paeonia suffruticosa control medicinal material solution, and the White Peony Root control medicinal material solution is 5-20 μL;

[0020] Optionally, the spotting volume of the gardenia glycoside reference solution, the liquiritigenin reference solution and the paeoniflorin reference solution is 3-20 μL.

[0021] Optionally, after unfolding, the method further comprises taking out, drying, spraying with vanillin ethanol sulfate and / or ethanol sulfate solution and then heating until color is developed;

[0022] After color development, it also includes inspection under fluorescent light.

[0023] Optionally, the step of preparing the sulfuric acid ethanol solution includes: mixing anhydrous ethanol and sulfuric acid, wherein the volume ratio of the anhydrous ethanol to the sulfuric acid is 9:1;

[0024] Optionally, the preparation steps of the vanillin sulfuric acid ethanol solution include: mixing ethanol and sulfuric acid to prepare a sulfuric acid ethanol solution, the volume ratio of ethanol to sulfuric acid is 9:1; the ethanol is analytical grade ethanol, and the sulfuric acid concentration is 9.5-10.5%; mixing the sulfuric acid ethanol solution and vanillin, the ratio of the weight of vanillin to the volume of the sulfuric acid ethanol solution is 1:20, and the weight and volume ratio is g / ml.

[0025] Optionally, the heating temperature is 100-110°C;

[0026] Optionally, the silica gel thin layer plate includes a silica gel G thin layer plate or a silica gel G254 thin layer plate.

[0027] Optionally, in the inspection step, the chromatogram of the test sample is compared with the chromatograms of white peony root control medicinal material, moutan bark control medicinal material, gardenia jasminoides control medicinal material, and liquorice control medicinal material.

[0028] Optionally, the Xuanyu Tongjing Decoction drug preparation includes a decoction of a Xuanyu Tongjing Decoction benchmark sample, a concentrated extract of a Xuanyu Tongjing Decoction benchmark sample, a lyophilized powder of a Xuanyu Tongjing Decoction benchmark sample, or a Xuanyu Tongjing Decoction preparation.

[0029] The technical solution of the present invention has the following advantages:

[0030] 1. The thin layer chromatography identification method of the relieving depression and tonifying menstruation drug preparation provided by the present invention comprises the following steps: preparing a test solution A, and based on the test solution A, using thin layer chromatography to identify gardenia, licorice, white peony root and peony bark; the thin layer chromatography identification method comprises: taking the test solution A, gardenia control medicinal material solution, licorice control medicinal material solution, peony bark control medicinal material solution, white peony root control medicinal material solution, gardenia glycoside reference solution, liquiritin reference solution, and paeoniflorin reference solution, respectively spotting them on the same silica gel thin layer plate, and developing them with ethyl acetate, formic acid, glacial acetic acid and water in a volume ratio of 15:(1-3):(1-3):2 as a developing solvent. The thin-layer identification method can simultaneously identify four medicinal materials, namely, Gardenia, Licorice, White Peony Root and Paeonia Suffruticosa, that is, the above four medicinal flavors can be simultaneously identified on the same thin-layer plate and the same developing agent system, which reduces the test time and reagent usage and improves the detection efficiency; the chromatogram obtained by using the thin-layer identification method has clear spots, good separation, strong specificity, no background interference, no interference from negative control substances and good durability.

[0031] Furthermore, ethyl acetate-formic acid-glacial acetic acid-water is used as a developing agent in a volume ratio of 15:(1-3):(1-3):2; the developing agent prepared under this volume ratio can be used immediately without stratification, so there is no need to place it at low temperature for a long time before use, which reduces the detection time.

[0032] 2. The thin layer identification method of the Xuanyutongjing drug preparation provided by the present invention, when preparing the test solution A of the present invention: the obtained residue is dissolved in water and then separated into solid and liquid, which is different from the prior art that requires the use of organic solvents for extraction and purification. The present invention directly uses water as a solvent, which simplifies the preparation steps of the test solution; at the same time, the residue evaporated after extraction with n-butanol can be placed for a long time, and methanol is added to dissolve it overnight and immediately added to dissolve it. The spots of paeoniflorin, liquiritin and gardenia in the thin layer chromatogram do not show obvious effects, and the thin layer identification method has low operating requirements. After the unfolded thin layer plate is heated and colored, the spots are clear, and the best effect is observed within 10 minutes.

[0033] Furthermore, the method for preparing the test solution for the thin layer identification method of the Chinese herbal compound preparation is usually complicated, the extraction steps are cumbersome, time-consuming and labor-intensive, and the test solutions for the thin layer identification method of different medicinal flavors are generally different. The present invention only requires weighing the sample once, and the sample is extracted and then prepared into the same test solution. Different medicinal flavors use the same test solution, which greatly improves the detection efficiency.

[0034] 3. In the thin layer identification method of the Xuanyu Tongjing drug preparation provided by the present invention, the color developer vanillin sulfuric acid ethanol solution has the best effect when prepared immediately before use, and the most spot information is displayed. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0036] Figure 1 This is the thin layer chromatogram obtained by specificity verification of Example 2 of the present invention;

[0037] Figure 2 This is a thin layer chromatogram obtained by investigating different developing agents in Example 2 of the present invention;

[0038] Figure 3 This is a thin layer chromatogram obtained by spotting sample quantity in Example 2 of the present invention;

[0039] Figure 4 This is a thin layer chromatogram obtained by investigating different ambient temperatures in Example 2 of the present invention;

[0040] Figure 5 This is a thin layer chromatogram obtained by investigating different environmental humidities in Example 2 of the present invention;

[0041] Figure 6 This is a thin layer chromatogram obtained by investigating the thin layer plate of Example 2 of the present invention;

[0042] Figure 7 This is a thin layer chromatogram obtained by investigating different sample spotting methods in Example 2 of the present invention;

[0043] Figure 8 This is a thin layer chromatogram obtained by investigating different batches of test samples in Example 2 of the present invention;

[0044] Fig. 9 It is the thin layer chromatogram obtained by the comparative experiment of Example 3 of the present invention. DETAILED DESCRIPTION

[0045] The following examples are provided for a better understanding of the present invention, but are not intended to limit the best mode of implementation, nor to limit the content and protection scope of the present invention. Any product identical or similar to the present invention obtained by anyone under the inspiration of the present invention or by combining the features of the present invention with other prior arts shall fall within the protection scope of the present invention.

[0046] If no specific experimental steps or conditions are specified in the examples, the conventional experimental steps or conditions described in the literature in the field can be used. If no manufacturer is specified for the reagents or instruments used, they are all conventional reagent products that can be obtained commercially.

[0047] Reference substance:

[0048] Paeoniflorin reference substance (source: China Food and Drug Inspection Institute, batch number: 110736-202246);

[0049] Gardenia jasminoides reference substance (source: China Food and Drug Administration, batch number: 110749-202320);

[0050] Liquorice glycoside reference substance (source: China Food and Drug Inspection Institute, batch number: 111610-202209);

[0051] Comparative medicinal materials:

[0052] White peony root control drug (Source: China Food and Drug Inspection Institute, batch number: (120905-202011);

[0053] Peony bark control medicinal material (source: China Food and Drug Inspection Institute, batch number: 121490-201603);

[0054] Licorice control medicinal material (source: China Food and Drug Inspection Institute, batch number: 120904-202021);

[0055] Gardenia jasminoides reference medicinal material (source: China Food and Drug Administration, batch number: 120986-202111); Preparation Example

[0056] Xuanyu Tongjing Decoction Granules (240503, 240504, 240505); Xuanyu Tongjing Decoction Standard Decoction (JZ23110602); Xuanyu Tongjing Decoction Standard Sample Concentrated Extract (NS01, NS02); Xuanyu Tongjing Decoction Standard Sample Freeze-dried Powder (DG01, DG02, DG03) are all made by our company.

[0057] The preparation method can be prepared by conventional methods in the art, as follows:

[0058] Xuanyu Tongjing Decoction Granules: Take the ten ingredients in the prescription, fry the mustard seeds and crush them, add water and the other nine ingredients and boil them twice. For the first time, add 8 times the water, soak for 30 minutes, keep it for 30 minutes after boiling, collect the aromatic water and the decoction, and filter the decoction; for the second time, add 6 times the water, keep it for 25 minutes after boiling, collect the decoction, filter the decoction, and combine the filtrate; encapsulate the aromatic water with beta-cyclodextrin, dry, grind it, and set aside; concentrate the filtrate into a clear paste, dry it, add appropriate amount of auxiliary materials and the above-mentioned beta-cyclodextrin inclusion compound, mix well, granulate, and make 1000g.

[0059] The standard decoction of Xuanyu Tongjing Decoction: take the ten ingredients in the prescription, fry the mustard seeds and crush them, add water and boil twice with the other nine ingredients. The first time, add 8 times the water, soak for 30 minutes, keep it for 30 minutes after boiling, and filter it; the second time, add 6 times the water, keep it for 25 minutes after boiling, filter it, and combine the filtrate to obtain the decoction.

[0060] Xuanyu Tongjing Decoction benchmark sample concentrated extract; take the ten ingredients in the prescription, fry the mustard seeds and crush them, add water and boil twice with the other nine ingredients, add 8 times the water for the first time, soak for 30 minutes, keep it for 30 minutes after boiling, and filter; add 6 times the water for the second time, keep it for 25 minutes after boiling, filter, combine the filtrate, and concentrate the filtrate into a clear paste.

[0061] Freeze-dried powder of the benchmark sample of Xuanyu Tongjing Decoction: take the ten ingredients in the prescription, fry the mustard seeds and crush them, add water and the other nine ingredients and boil them twice. The first time, add 8 times the water, soak for 30 minutes, keep it for 30 minutes after boiling, and filter it; the second time, add water, keep it for 25 minutes after boiling, filter it, combine the filtrate, pre-freeze the decoction below -80℃, and then freeze-dry it to obtain the powder.

[0062] The prescription of Xuanyu Tongjing Decoction is: 18.65g of white peony root with wine, 18.65g of angelica with wine, 18.65g of peony bark, 11.19g of stir-fried gardenia, 7.46g of stir-fried mustard seeds, 3.73g of bupleurum, 3.73g of cyperus with wine, 3.73g of turmeric with vinegar, 3.73g of scutellaria with wine, and 3.73g of licorice.

[0063] Example 1

[0064] This embodiment provides a thin layer identification method for a drug preparation for relieving depression and promoting menstruation, comprising:

[0065] 1. Preparation of test solution A: Take 7.5 g of Xuanyu Tongjing Decoction reference sample freeze-dried powder (DG01), add 30 ml of methanol, ultrasonically treat for 15 minutes, filter, evaporate the filtrate to dryness, dissolve the residue in 30 ml of water, filter, extract the filtrate twice with water-saturated n-butanol, 30 ml each time, combine the n-butanol solution, wash 3 times with water saturated with n-butanol, 30 ml each time, evaporate the n-butanol solution, dissolve the residue in 2 ml of methanol to prepare test solution A.

[0066] 2. Preparation of control medicinal material solution: Take 1 g each of gardenia control medicinal material, licorice control medicinal material, peony bark control medicinal material and white peony root control medicinal material, add 30 ml of methanol respectively, treat with ultrasound for 15 minutes, filter, evaporate the filtrate to dryness, dissolve the residue in 30 ml of water, filter, extract the filtrate with water-saturated n-butanol twice, 30 ml each time, combine the n-butanol solution, wash with water saturated with n-butanol three times, 30 ml each time, evaporate the n-butanol solution, dissolve the residue in 2 ml of methanol to prepare gardenia control medicinal material solution, licorice control medicinal material solution, peony bark control medicinal material solution and white peony root control medicinal material solution.

[0067] 3. Preparation of reference solution: Take peony inoside reference substance, add methanol, and prepare a solution containing 1 mg of peony inoside reference substance per 1 mL; take gardenia inoside reference substance, add methanol, and prepare a solution containing 1 mg of gardenia inoside reference substance per 1 mL; take licorice inoside reference substance, add methanol, and prepare a solution containing 1 mg of licorice inoside reference substance per 1 mL.

[0068] 4. Chromatographic conditions: According to the thin layer chromatography method (General Rule 0502), the above test solution A (5 μL), gardenia control medicinal material solution (5 μL), licorice control medicinal material solution (5 μL), peony bark control medicinal material solution (5 μL) and white peony root control medicinal material solution (5 μL); gardenia glycoside reference solution (3 μL), liquiritigenin reference solution (3 μL) and paeoniflorin reference solution (3 μL) were respectively spotted on the same silica gel G thin layer plate (Merck plate), and ethyl acetate-formic acid-glacial acetic acid-water (15:1:1:2) was used as the developing agent. The plate was developed, taken out, dried, sprayed with 5% vanillin sulfuric acid ethanol solution (ethanol and 5% vanillin sulfuric acid solution were mixed, and the volume ratio of ethanol to vanillin sulfuric acid solution was 9:1), and then heated to 105°C until the spots were clearly colored and inspected under a fluorescent lamp.

[0069] In the test sample chromatogram, a fluorescent spot of the same color should appear at the corresponding position in the reference medicinal material chromatogram; and a fluorescent spot of the same color should appear at the corresponding position in the reference sample chromatogram.

[0070] Example 2

[0071] This embodiment provides a thin layer chromatography identification method for a relieving depression and tonifying menstruation drug preparation, which is investigated using different chromatographic conditions, including:

[0072] 1. Exclusiveness verification

[0073] (1) Preparation of negative control solution:

[0074] The four-negative test solution lacking white peony root, peony bark, gardenia and licorice (N-BMZG) was prepared by taking the six ingredients of the prescription (excluding white peony root, peony bark, gardenia and licorice) according to the preparation method of the lyophilized powder of the Xuanyu Tongjing Decoction benchmark sample in the preparation example.

[0075] Double-negative test solution lacking white peony root and peony bark (N-BM): Take the eight ingredients in the prescription (except white peony root and peony bark) and prepare it according to the preparation method of the lyophilized powder of the benchmark sample of Xuanyu Tongjing Decoction in the preparation example.

[0076] Double negative test solution lacking Gardenia and Licorice (N-ZG): Take the eight ingredients in the prescription (except Gardenia and Licorice) and prepare it according to the preparation method of the lyophilized powder of the Xuanyu Tongjing Decoction benchmark sample in the preparation example.

[0077] Negative solution lacking white peony root: Take the nine ingredients in the prescription (except white peony root) and prepare it according to the preparation method of the freeze-dried powder of the benchmark sample of Xuanyu Tongjing Decoction in the preparation example.

[0078] Negative solution lacking peony bark (N-BM): Take the nine ingredients in the prescription (except peony bark) and prepare it according to the preparation method of the lyophilized powder of the Xuanyu Tongjing Decoction benchmark sample in the preparation example.

[0079] Negative solution lacking Gardenia (N-ZZ): Take the nine ingredients in the prescription (except Gardenia) and prepare it according to the preparation method of the freeze-dried powder of the benchmark sample of Xuanyu Tongjing Decoction in the preparation example.

[0080] Negative solution lacking licorice (N-GC): Take the nine ingredients in the prescription (excluding licorice) and prepare it according to the preparation method of the lyophilized powder of the Xuanyu Tongjing Decoction benchmark sample in the preparation example.

[0081] The negative solutions prepared above were taken and prepared according to the preparation method of the test solution A in Example 1, respectively, into four negative test solutions lacking white peony root, peony bark, gardenia and licorice, double negative test solutions lacking white peony root and peony bark, double negative test solutions lacking gardenia and licorice, negative test solutions lacking white peony root, negative test solutions lacking peony bark, negative test solutions lacking gardenia and negative test solutions lacking licorice.

[0082] (2) Chromatographic conditions: According to the thin layer chromatography method (General Rule 0502), the above-mentioned four-negative test solution lacking white peony root, peony bark, gardenia and liquorice (15 μL), the double-negative test solution lacking white peony root and peony bark (15 μL), the double-negative test solution lacking gardenia and liquorice (15 μL), the negative solution lacking white peony root (15 μL), the negative solution lacking peony bark (15 μL), the negative solution lacking gardenia (15 μL), the negative solution lacking liquorice (15 μL), the white peony root control medicinal material solution (5 μL), the peony root control medicinal material solution (5 μL), the gardenia control medicinal material solution (5 μL), the liquorice control medicinal material solution (15 μL) in Example 1 were taken respectively. The material solution (5 μL), peony glycoside reference solution (3 μL), gardenia glycoside reference solution (3 μL), licorice glycoside reference solution (3 μL), and test solution A (3 μL, 5 μL, 8 μL) were spotted on the same silica gel G thin layer plate (Merck plate), and ethyl acetate-formic acid-glacial acetic acid-water, volume ratio (15:1:1:2) was used as the developing agent. The plate was developed, taken out, dried, and sprayed with 5% vanillin sulfuric acid ethanol solution (ethanol and 5% vanillin sulfuric acid solution were mixed, and the volume ratio of ethanol to vanillin sulfuric acid solution was 9:1). The plate was heated at 105°C until the spots were clearly colored and examined under a fluorescent lamp. The results are as follows Figure 1 As shown,

[0083] exist Figure 1In the table, 1 is a negative solution lacking white peony root, 2 is a negative solution lacking peony bark, 3 is a negative solution lacking gardenia, 4 is a negative solution lacking liquorice, 5 is a double negative test solution lacking white peony root and peony bark, 6 is a double negative test solution lacking gardenia and liquorice, 7 is a quadruple negative test solution lacking white peony root, peony bark, gardenia and liquorice, 8 is test solution A (3 μL), 9 is test solution A (5 μL), 10 is test solution A (8 μL), 11 is paeoniflorin reference solution, 12 is gardenia reference solution, 13 is licorice reference solution, 14 is white peony reference medicinal material solution, 15 is peony bark reference medicinal material solution, 16 is gardenia control medicinal material solution, and 17 is liquorice control medicinal material solution.

[0084] Depend on Figure 1 It can be seen that in the chromatogram of the test sample, spots of the same color appear at the corresponding positions of the chromatograms of the white peony control medicinal material, the peony bark control medicinal material, and the paeoniflorin reference solution when observed under sunlight; spots of the same color appear at the corresponding positions of the chromatograms of the gardenia control medicinal material and the gardeniaflorin reference solution; spots of the same color appear at the corresponding positions of the chromatograms of the licorice control medicinal material and the licorice reference solution, the spot separation is good and there is no interference from the negative test sample solution, which meets the specificity requirements.

[0085] 2. Investigation of different developing agents

[0086] (1) Chloroform-ethyl acetate-methanol-concentrated ammonia test solution (8:1:4:1);

[0087] (2) ethyl acetate-formic acid-glacial acetic acid-water (15:1:1:2);

[0088] (3) ethyl acetate-formic acid-glacial acetic acid-water (15:3:1:2);

[0089] (4) ethyl acetate-formic acid-glacial acetic acid-water (15:1:3:2);

[0090] Compared with Example 1, the difference is that different developing agents (1), (2), (3) and (4) are used for developing respectively, instead of using ethyl acetate-formic acid-glacial acetic acid-water (15: (1-3): (1-3): 2) as the developing agent in Example 1. The results are shown in FIG. Figure 2 As shown. Among them, Figure 2 a in the figure is the thin layer chromatogram obtained by developing solvent (1), Figure 2 In a, 1 is the double-negative test solution lacking white peony root and peony bark, 2 is the double-negative test solution lacking gardenia and liquorice, 3 and 4 are both test solution A, 5 is paeoniflorin reference solution, 6 is gardenia glycoside reference solution, 7 is licorice reference solution, 8 is peony bark reference medicinal material solution, 9 is white peony root reference medicinal material solution, 10 is gardenia jasminoides reference medicinal material solution, and 11 is liquorice control medicinal material solution.

[0091] Figure 2 b in the figure is the thin layer chromatogram obtained by using the developing agent (2). Figure 2 In b, 1 is the test solution A, 2 is the paeoniflorin reference solution, 3 is the gardenoside reference solution, 4 is the liquiritifolia reference solution, 5 is the white peony root reference solution, 6 is the peony bark reference solution, 7 is the gardenia jasminoides reference solution, and 8 is the licorice reference solution.

[0092] Figure 2 c in the figure is the thin layer chromatogram obtained by the developing agent (3), Figure 2 In c, 1 is paeoniflorin reference solution, 2 is gardenia glycoside reference solution, 3 is liquiritirubin reference solution, 4 is test solution A, 5 is white peony root reference medicinal material solution, 6 is peony bark reference medicinal material solution, 7 is gardenia jasminoides reference medicinal material solution, and 8 is licorice reference medicinal material solution.

[0093] Figure 2 d in the figure is the thin layer chromatogram obtained by the developing agent (4), Figure 2 In d, 1 is paeoniflorin reference solution, 2 is gardenoside reference solution, 3 is liquiritirubin reference solution, 4 is white peony root reference medicinal material solution, 5 is peony bark reference medicinal material solution, 6 is gardenia jasminoides reference medicinal material solution, 7 is licorice reference medicinal material solution, and 8 is test solution A.

[0094] The above Figure 2 The double-negative test solution lacking white peony root and moutan bark, the double-negative test solution lacking gardenia jasminoides and licorice, the test solution A, the reference solution, and the reference medicinal material solution are all prepared in “1. Specificity Verification”.

[0095] Depend on Figure 2 It can be seen from the ad in that when chloroform-ethyl acetate-methanol-concentrated ammonia solution (8:1:4:1) is used as the developing solvent, the liquiritin development distance is short and the color is similar to the nearby spots, and the separation effect is poor. When ethyl acetate-formic acid-glacial acetic acid-water is used as the developing solvent, the ratio is (15:1:1:2), (15:3:1:2), (15:1:3:2), the spot information is more and the separation degree is good. Therefore, the present invention selects ethyl acetate-formic acid-glacial acetic acid-water as the developing solvent, and the volume ratio is 15:(1-3):(1-3):2.

[0096] 3. Sample quantity inspection

[0097] Compared with Example 1, the difference is that the four negative test solutions lacking white peony root, peony bark, gardenia and licorice (15 μL), the double negative test solution lacking white peony root and peony bark (15 μL), the double negative test solution lacking gardenia and licorice (15 μL), the negative solution lacking white peony root (15 μL), the negative solution lacking peony bark (15 μL), the negative solution lacking gardenia (15 μL), and the negative solution lacking licorice (15 μL) were also aspirated respectively; the test solution A (3 μL, 5 μL, 8 μL) were spotted on the same silica gel G thin layer plate (Merck plate); the results are as follows Figure 3 The negative solution is prepared in "1. Specificity Verification".

[0098] exist Figure 3 In the table, 1 is a negative solution lacking white peony root, 2 is a negative solution lacking peony bark, 3 is a negative solution lacking gardenia, 4 is a negative solution lacking liquorice, 5 is a double negative test solution lacking white peony root and peony bark, 6 is a double negative test solution lacking gardenia and liquorice, 7 is a quadruple negative test solution lacking white peony root, peony bark, gardenia and liquorice, 8 is test solution A (3 μL), 9 is test solution A (5 μL), 10 is test solution A (8 μL), 11 is paeoniflorin reference solution, 12 is gardenia reference solution, 13 is licorice reference solution, 14 is white peony reference medicinal material solution, 15 is peony bark reference medicinal material solution, 16 is gardenia control medicinal material solution, and 17 is liquorice control medicinal material solution.

[0099] Depend on Figure 3 It can be seen that the thin layer identification method provided by the present invention can obtain good detection results under different spotting amounts, and the spots are clear, indicating that the method has good durability for different spotting amounts.

[0100] 4. Investigation of different ambient temperatures

[0101] (1) The ambient temperature is 6°C;

[0102] (2) The ambient temperature is 23.6°C;

[0103] (3) The ambient temperature is 45°C;

[0104] Under the conditions (1) to (3), thin layer identification was performed according to the thin layer identification method of Example 1. The results are as follows: Figure 4 As shown. Among them, Figure 4 a in the figure is the TLC obtained at ambient temperature (1), Figure 4 1 to 3 in a are all test solution A, 4 is paeoniflorin reference solution, 5 is gardenia glycoside reference solution, 6 is liquiritirubin reference solution, 7 is white peony root reference solution, 8 is peony bark reference solution, 9 is gardenia jasminoides reference solution, and 10 is licorice reference solution.

[0105] Figure 4b in the figure is the thin layer chromatogram obtained at ambient temperature (2). Figure 4 In b, 1 is the test solution A, 2 is the paeoniflorin reference solution, 3 is the gardenoside reference solution, 4 is the liquiritifolia reference solution, 5 is the white peony root reference solution, 6 is the peony bark reference solution, 7 is the gardenia jasminoides reference solution, and 8 is the licorice reference solution.

[0106] Figure 4 c in the figure is the thin layer chromatogram obtained at ambient temperature (3), Figure 4 In b, 1 is paeoniflorin reference solution, 2 is gardenia glycoside reference solution, 3 is liquiritirubin reference solution, 4 is test solution A, 5 is white peony root reference medicinal material solution, 6 is peony bark reference medicinal material solution, 7 is gardenia jasminoides reference medicinal material solution, and 8 is licorice reference medicinal material solution.

[0107] Depend on Figure 4 It can be seen from ac in that the thin layer identification method provided by the present invention can obtain good detection effect at different temperatures, and there is no obvious difference in the separation effect and spot display in the chromatogram, indicating that the thin layer identification method provided by the present invention has good durability at an ambient temperature of 6-45°C.

[0108] 5. Investigation of humidity in different environments

[0109] (1) Ambient humidity is 32% RH;

[0110] (2) Ambient humidity is 88% RH;

[0111] Under the conditions of (1) and (2), thin layer identification was performed according to the thin layer identification method of Example 1. The results are as follows: Figure 5 As shown. Among them, Figure 5 a in the figure is the thin layer chromatogram obtained at ambient humidity (1), Figure 5 In a, 1 is a negative solution lacking white peony root, 2 is a negative solution lacking moutan bark, 3 is a negative solution lacking gardenia, 4 is a negative solution lacking licorice, 5 is test solution A, 6 is a paeoniflorin reference solution, 7 is a gardenia reference solution, 8 is a licorice reference solution, 9 is a white peony reference medicinal material solution, 10 is a moutan bark reference medicinal material solution, 11 is a gardenia reference medicinal material solution, and 12 is a licorice reference medicinal material solution. The above negative solutions are prepared in "1. Specificity Verification".

[0112] Figure 5 b in the figure is the thin layer chromatogram obtained at ambient humidity (2). Figure 5 In b, 1 is paeoniflorin reference solution, 2 is gardenia glycoside reference solution, 3 is liquiritirubin reference solution, 4 is test solution A, 5 is white peony root reference medicinal material solution, 6 is peony bark reference medicinal material solution, 7 is gardenia jasminoides reference medicinal material solution, and 8 is licorice reference medicinal material solution.

[0113] Depend on Figure 5 It can be seen from ab in that the thin layer identification method provided by the present invention can obtain good detection effects under different humidity conditions, and there is no obvious difference in the separation effect and spot display in the chromatogram, indicating that the thin layer identification method provided by the present invention has good durability under an environmental humidity of 32-88% RH.

[0114] 6. Inspection of thin layer boards produced by different manufacturers

[0115] According to the sampling amount of Example 1, the test solution A, the corresponding reference solution and the corresponding reference medicinal material solution were respectively aspirated and spotted on different thin layer plates: Yantai plate (Yantai Huayang New Material Technology Co., Ltd., silica gel G plate, batch number 20230728), Qingdao plate (manufactured by Qingdao Ocean Chemical Co., Ltd., silica gel G plate, batch number 20230801), Merck plate (Merck, silica gel G plate, batch number HX28729426); then developed according to the chromatographic conditions. The results are as follows Figure 6 As shown. Among them, Figure 6 a in the figure is the thin layer chromatogram obtained from the Yantai plate. Figure 6 In a, 1 is paeoniflorin reference solution, 2 is gardenia glycoside reference solution, 3 is liquiritirubin reference solution, 4 is test solution A, 5 is white peony root reference medicinal material solution, 6 is peony bark reference medicinal material solution, 7 is gardenia jasminoides reference medicinal material solution, and 8 is licorice reference medicinal material solution.

[0116] Figure 6 b in the figure is the thin layer chromatogram obtained from Qingdao plate. Figure 6 In b, 1 is paeoniflorin reference solution, 2 is gardenia glycoside reference solution, 3 is liquiritirubin reference solution, 4 is test solution A, 5 is white peony root reference medicinal material solution, 6 is peony bark reference medicinal material solution, 7 is gardenia jasminoides reference medicinal material solution, and 8 is licorice reference medicinal material solution.

[0117] Figure 6 The c in the figure is the thin layer chromatogram obtained from the Merck plate. Figure 6 In c, 1 is the test solution A, 2 is the paeoniflorin reference solution, 3 is the gardenia glycoside reference solution, 4 is the liquiritirubin reference solution, 5 is the white peony root reference medicinal material solution, 6 is the peony bark reference medicinal material solution, 7 is the gardenia jasminoides reference medicinal material solution, and 8 is the licorice reference medicinal material solution.

[0118] Depend on Figure 6 It can be seen from ac in that the thin layer identification method provided by the present invention can obtain good detection effects when using different thin layer plates, and there is no obvious difference in the separation effect and spot display in the chromatogram, indicating that the thin layer identification method provided by the present invention has good durability for thin layer plates produced by different manufacturers.

[0119] 7. Different sampling methods

[0120] (1) The spot shape is a strip;

[0121] (2) The dot pattern is a circular dot;

[0122] The thin layer identification was performed according to the thin layer identification method of Example 1 using the spotting methods (1) and (2) respectively. The results are as follows: Figure 7 As shown. Among them, Figure 7 a in the figure is the thin layer chromatogram obtained by spotting method (1). Figure 7 In a, 1 is the test solution A, 2 is the paeoniflorin reference solution, 3 is the gardenia glycoside reference solution, 4 is the liquiritirubin reference solution, 5 is the white peony root reference solution, 6 is the peony bark reference solution, 7 is the gardenia jasminoides reference solution, and 8 is the licorice reference solution.

[0123] Figure 7 b in the figure is the thin layer chromatogram obtained by spot sampling method (2). Figure 7 In b, 1 is liquiritin reference solution, 2 is gardenia glycoside reference solution, 3 is paeoniflorin reference solution, 4 is white peony root reference medicinal material solution, 5 is peony bark reference medicinal material solution, 6 is gardenia jasminoides reference medicinal material solution, 7 is licorice reference medicinal material solution, and 8 is test solution A.

[0124] Depend on Figure 7 It can be seen from ab in that the thin layer identification method provided by the present invention can obtain good detection effect under different spotting modes, and there is no obvious difference in the separation effect and spot display in the chromatogram, indicating that the thin layer identification method provided by the present invention has good durability under different spotting modes.

[0125] 8. Inspection of different batches of test products

[0126] (1) The test sample was the standard decoction of Xuanyu Tongjing Decoction, batch number JZ23110602. The test solution was prepared according to the "Preparation of the Test Solution" in Example 1. The thin layer chromatogram of the test sample was obtained according to the thin layer identification method in Example 1. The results are shown in FIG. Figure 8 ;

[0127] (2) The test sample was Xuanyu Tongjing Decoction concentrated extract, batch number NS01 and NS02, and the test solution was prepared according to the "Preparation of Test Solution" in Example 1. The thin layer chromatogram of the test sample was obtained according to the thin layer identification method in Example 1. The results are shown in Figure 8 ;

[0128] (3) The test sample was the lyophilized powder of the Xuanyu Tongjing Decoction reference sample, with batch numbers DG01, DG02 and DG03. The test solution was prepared according to the "Preparation of the Test Solution" in Example 1. The thin layer chromatogram of the test sample was obtained according to the thin layer identification method in Example 1. The results are shown in FIG. Figure 8;

[0129] (4) The test sample was Xuanyu Tongjing Decoction Granules, batch numbers 240503, 240504 and 240505. The test sample solution was prepared according to the "Preparation of Test Sample Solution" in Example 1. The thin layer chromatogram of the test sample was obtained according to the thin layer identification method in Example 1. The results are shown in FIG. Figure 8 ;

[0130] in, Figure 8 S1 is paeoniflorin reference solution, S2 is gardenia glycoside reference solution, S3 is liquiritilicate reference solution, 1 is white peony root reference medicinal material solution, 2 is peony bark reference medicinal material solution, 3 is gardenia jasminoides reference medicinal material solution, 4 is licorice reference medicinal material solution, 5 is Xuanyu Tongjing Decoction standard decoction (JZ23110602), 6-7 correspond to Xuanyu Tongjing Decoction concentrated extracts of batch numbers NS01 and NS02, 8-10 correspond to Xuanyu Tongjing Decoction standard samples (lyophilized powder) of batch numbers DG01, DG02, and DG03, and 11-13 correspond to Xuanyu Tongjing Decoction granules of batch numbers 240503, 240504, and 240505, respectively.

[0131] Depend on Figure 8 It can be seen that the thin layer identification method provided by the present invention can be applied to the Xuanyu Tongjing Decoction standard decoction, Xuanyu Tongjing Decoction concentrated extract, Xuanyu Tongjing Decoction standard sample freeze-dried powder, and Xuanyu Tongjing Decoction granules.

[0132] Example 3

[0133] This embodiment provides a comparative experiment, which is as follows:

[0134] 1. Investigation of different test solution preparation methods

[0135] (1) Preparation method of test solution a: Take 7.5 g of lyophilized powder of Xuanyu Tongjing Decoction reference sample, add 50 ml of methanol, ultrasonically treat for 30 minutes, filter, evaporate the filtrate to dryness, add 2 ml of methanol to dissolve the residue, and prepare test solution a.

[0136] (2) Preparation method of test solution b: Take 7.5 g of lyophilized powder of Xuanyu Tongjing Decoction reference sample, add 50 ml of water, ultrasonically treat for 30 min, filter, add hydrochloric acid to adjust the pH to 2, add ethyl acetate and shake to extract twice, 30 ml each time, combine the ethyl acetate, evaporate to dryness in a water bath, add 2 ml of methanol to dissolve the residue, and prepare test solution b.

[0137] (3) Preparation of test solution A: Take 7.5 g of the lyophilized powder of the Xuanyu Tongjing Decoction reference sample, add 30 ml of methanol, and ultrasonically treat for 15 min. Filter, evaporate the filtrate to dryness, dissolve the residue in 30 ml of water, filter, extract the filtrate twice with saturated n-butanol with water, each time with 30 ml, combine the n-butanol solutions, wash three times with water saturated with n-butanol, each time with 30 ml, evaporate the n-butanol solution to dryness, and dissolve the residue in 2 ml of methanol to prepare test solution A.

[0138] According to the sampling amount of Example 1, the above test solution was respectively aspirated, and thin layer identification was performed according to the thin layer identification method of Example 1. The results are as follows Fig. 9 shown.

[0139] in, Fig. 9 1 is paeoniflorin reference solution, 2 is gardenia glycoside reference solution, 3 is liquiritirubin reference solution, 4 is white peony root reference medicinal material solution, 5 is peony bark reference medicinal material solution, 6 is gardenia jasminoides reference medicinal material solution, 7 is licorice reference medicinal material solution, 8 is test solution a, 9 is test solution b, and 10 is test solution A.

[0140] Depend on Fig. 9 It can be seen that in the chromatogram of the preparation method of the test solution A in the thin layer identification method provided by the present invention, there are more spot information and better separation; while in the chromatograms of the test solutions a and b prepared by the test solution preparation methods a and b, the spots are not clear and the spot information is less.

[0141] By comparing with comparative experiment 1, it is shown that the thin layer chromatogram obtained by the thin layer identification method provided by the present invention has rich spot information, good separation, clear color development of the chromatogram, and accurate identification results, which is conducive to the simultaneous identification of the four medicinal flavors of Gardenia, White Peony Root, Paeonia Suffruticosa Root and Licorice in the Xuanyu Tongjing drug preparation.

[0142] Obviously, the above embodiments are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived from these are still within the scope of protection of the invention.

Claims

1. A thin layer identification method for a drug preparation for relieving depression and promoting menstruation, characterized in that: The steps include: A test solution A is prepared, and based on the test solution A, a thin layer chromatography method is used to simultaneously identify Gardenia, Licorice, White Peony Root and Paeonia Suffruticosa Root in the Xuanyu Tongjing drug preparation; According to thin layer chromatography: The test solution A, gardenia control medicinal material solution, licorice control medicinal material solution, peony bark control medicinal material solution, white peony control medicinal material solution, gardenia glycoside reference solution, liquiritin reference solution, and paeoniflorin reference solution were spotted on the same silica gel thin layer plate, and developed with ethyl acetate, formic acid, glacial acetic acid and water in a volume ratio of 15:(1-3):(1-3):2 as the developing solvent.

2. The thin layer identification method of the drug preparation for relieving depression and promoting menstruation according to claim 1, characterized in that: In the developing solvent, the volume ratio of ethyl acetate, formic acid, glacial acetic acid and water is 15:1:1:

2.

3. The thin layer identification method of the Xuanyutongjing medicinal preparation according to any one of claims 1-2, characterized in that: The preparation process of the test solution A comprises: taking a test sample of a Xuanyutongjing drug preparation, adding methanol to dissolve it, and then separating the solid and liquid, taking the liquid and evaporating it to dryness, adding water to dissolve the obtained residue, and then separating the solid and liquid, taking the liquid and adding water-saturated n-butanol to extract it, taking the liquid and washing it with water saturated with n-butanol, taking the n-butanol washing liquid and evaporating it to dryness, and adding methanol to re-dissolve the obtained residue; Optionally, take 5-10g weight portion of the Xuanyu Tongjing drug preparation test sample, add 10-50ml volume portion of methanol to dissolve it and separate the solid and liquid, take the liquid and evaporate it to dryness, add 20-40ml volume portion of water to dissolve the obtained residue and separate the solid and liquid, take the liquid and add water-saturated n-butanol to extract 1-3 times, 20-50ml volume portion each time, combine the extracts, take the liquid and wash it with water saturated with n-butanol 1-3 times, 20-50ml volume portion each time, take the n-butanol washing liquid and evaporate it to dryness, and add 1-3ml volume portion of methanol to the obtained residue to redissolve it; the ratio of weight portion to volume portion is g / ml.

4. The thin layer identification method of the drug preparation for relieving depression and promoting menstruation according to claim 3, characterized in that: The method further comprises the steps of preparing the gardenia control medicinal material solution, the liquorice control medicinal material solution, the peony bark control medicinal material solution and the white peony root control medicinal material solution according to the preparation process of the test solution A; Optionally, 0.5-2 g weight portion of each of Gardenia jasminoides, Licorice root, Moutan bark and White Peony root are taken, and 10-50 ml volume portions of methanol are added to each of them for dissolution, followed by solid-liquid separation. The liquid is evaporated to dryness, 20-40 ml volume portions of water are added to the obtained residue for dissolution, followed by solid-liquid separation, the liquid is extracted with water-saturated n-butanol for 1-3 times, 20-50 ml volume portions each time, the extracts are combined, the liquid is washed with water saturated with n-butanol for 1-3 times, 20-50 ml volume portions each time, the n-butanol washing liquid is evaporated to dryness, and 1-3 ml volume portions of methanol are added to the obtained residue for redissolution to prepare the corresponding control medicinal material solutions; the ratio of weight portions to volume portions is g / ml.

5. The thin layer identification method of the drug preparation for relieving depression and tonifying menstruation according to any one of claims 1 to 4, characterized in that: The paeoniflorin reference substance solution is prepared by dissolving the paeoniflorin reference substance in methanol, wherein 1-5 mg of the paeoniflorin reference substance is dissolved in every 1 mL of methanol; The gardenoside reference substance solution is prepared by dissolving the gardenoside reference substance in methanol, wherein 1-5 mg of the gardenoside reference substance is dissolved in every 1 mL of methanol; The liquiritin reference substance solution is prepared by dissolving the liquiritin reference substance in methanol, with 1-5 mg of the liquiritin reference substance being dissolved in every 1 mL of methanol.

6. The thin layer identification method of the drug preparation for relieving depression and tonifying menstruation according to any one of claims 1 to 5, characterized in that: The sample volume of the test solution A is 3-8 μL; and / or, The spotting volume of the Gardenia jasminoides control medicinal material solution, the Licorice control medicinal material solution, the Moutan bark control medicinal material solution and the White Peony Root control medicinal material solution is 5-20 μL; and / or, The spotting volume of the gardenia glycoside reference solution, liquiritigenin reference solution and paeoniflorin reference solution was 3-20 μL.

7. The thin layer identification method of the drug preparation for relieving depression and tonifying menstruation according to any one of claims 1 to 6, characterized in that: After unfolding, the method further includes taking out, drying, spraying with vanillin ethanol sulfate and / or ethanol sulfate solution and then heating until color is developed; After color development, it also includes inspection under fluorescent light.

8. The thin layer identification method of the drug preparation for relieving depression and promoting menstruation according to claim 7, characterized in that: The preparation step of the sulfuric acid ethanol solution comprises: mixing anhydrous ethanol and sulfuric acid, wherein the volume ratio of the anhydrous ethanol to the sulfuric acid is 9:1; and / or, The preparation steps of the vanillin sulfuric acid ethanol solution include: mixing ethanol and sulfuric acid to prepare sulfuric acid ethanol solution, wherein the volume ratio of ethanol to sulfuric acid is 9:1, the ethanol is analytical grade ethanol, and the concentration of sulfuric acid is 9.5-10.5%; mixing the sulfuric acid ethanol solution and vanillin, wherein the weight of the vanillin and the weight volume ratio of the sulfuric acid ethanol solution is 1:20, and the weight to volume ratio is g / ml; and / or, The heating temperature is 100-110° C.; and / or, The silica gel thin layer plate includes a silica gel G thin layer plate or a silica gel G254 thin layer plate, and / or, In the inspection step, the chromatogram of the test sample is compared with the chromatograms of white peony root control medicinal material, moutan bark control medicinal material, gardenia jasminoides control medicinal material, and liquorice control medicinal material.

9. The thin layer identification method of the drug preparation for relieving depression and tonifying menstruation according to any one of claims 1 to 8, characterized in that: The Xuanyu Tongjing Decoction drug preparation includes a water decoction of a Xuanyu Tongjing Decoction reference sample, a concentrated extract of a Xuanyu Tongjing Decoction reference sample, a lyophilized powder of a Xuanyu Tongjing Decoction reference sample or a Xuanyu Tongjing Decoction preparation.

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