Low-temperature disinfection gel and low-temperature disinfection method

Abstract

The invention relates to a low-temperature disinfection gel and a low-temperature disinfection method.The low-temperature disinfection gel is prepared from, by volume, 70-80% of a solution A, 10-15% of a solution B and 10-15% of a solution C. The solution A is an aqueous solution of polyvinyl alcohol, and the solution C is an aqueous solution of polyvinyl alcohol. The solution B is a solution formed by dissolving betaine in a 3-(cyclohexyl) aminopropanesulfonic acid aqueous solution and then hydrolyzing, and the solution C is an aqueous solution of hexadecyl trimethyl ammonium chloride. When the low-temperature disinfection gel is matched with a disinfectant for disinfection, lasting and efficient sterilization and antibacterial effects can be achieved, and the low-temperature disinfection gel has an extremely good application prospect in the field of cold-chain food disinfection.

Description

Technical Field

[0001] The invention relates to low-temperature disinfection technology, in particular to a low-temperature disinfection gel and a low-temperature disinfection method. Background Art

[0002] In recent years, the market demand for cold chain products such as meat, fruit, aquatic products, and frozen foods has grown rapidly, driving the rapid development of cold chain food production and operation companies. The production process of cold chain food includes breeding, fishing, processing, storage, and transportation, which are susceptible to microbial contamination, leading to food corruption and food safety issues. In order to ensure food safety and prevent cross contamination, the surface of low-temperature refrigerated objects needs to be disinfected.

[0003] Chlorine-containing disinfectants are commonly used in cold chain food production and operation, and are suitable for the disinfection of surfaces, fruits and vegetables, and tableware. Generating sodium hypochlorite disinfectant on site to disinfect equipment and facilities is a fast, convenient and quick way.

[0004] Commonly used low-temperature disinfectants usually contain alcohol organic solvents such as ethanol, which are designed to lower the freezing point of the disinfectant so that it can remain liquid and stable in low-temperature environments. However, sodium hypochlorite has extremely strong oxidizing properties and is easily reacted with alcohols when dissolved in organic solvents such as ethanol. The effective chlorine content will decrease rapidly, affecting the disinfection effect. Therefore, there is a contradiction between alcohol organic solvents and the disinfection effect in existing low-temperature disinfectants, making it difficult to achieve both a low freezing point and efficient disinfection. Summary of the invention

[0005] The purpose of the present invention is to overcome the problem of poor disinfection effect in the existing low-temperature disinfection field and to provide a low-temperature disinfection gel and a low-temperature disinfection method.

[0006] The specific plan is as follows:

[0007] A low-temperature disinfection gel comprises the following components in volume percentage: 70-80% of A solution, 10-15% of B solution, and 10-15% of C solution, wherein the A solution is an aqueous solution of polyvinyl alcohol, the B solution is a solution formed by hydrolyzing betaine dissolved in a 3-(cyclohexyl)aminopropanesulfonic acid aqueous solution, and the C solution is an aqueous solution of hexadecyltrimethylammonium chloride.

[0008] Furthermore, the concentration of polyvinyl alcohol in the A solution is 1-5wt%.

[0009] Furthermore, the preparation method of the B solution is: dissolving 0.5-1wt% betaine in a 3-(cyclohexyl)aminopropanesulfonic acid solution containing 10-20mM, pH=9-11, stirring at 35-40°C for 2-5 hours to cause a hydrolysis reaction to obtain the B solution.

[0010] Furthermore, the concentration of hexadecyltrimethylammonium chloride in the C solution is 0.5-1 wt %.

[0011] Furthermore, the low-temperature disinfection gel consists of the A solution, the B solution, the C solution, and a pH regulator.

[0012] Furthermore, the pH of the low-temperature disinfection gel is 7-9.

[0013] The present invention also protects the use of the low-temperature disinfection gel in the disinfection of cold chain products.

[0014] The present invention also protects a low-temperature sterilization method, which uses the low-temperature sterilization gel, comprising the following steps:

[0015] S1. Use disinfectant to disinfect the items to be disinfected;

[0016] S2, spraying the low-temperature disinfection gel on the surface of the disinfected object to be disinfected, so that the low-temperature disinfection gel forms a coating on the surface of the object to be disinfected;

[0017] S3, disinfecting the coated article again with disinfectant.

[0018] Furthermore, the disinfectant contains sodium hypochlorite. Preferably, the concentration of sodium hypochlorite in the disinfectant is 100-3000 ppm. More preferably, the disinfectant is prepared according to the following method: preparing a sodium chloride aqueous solution with a concentration of 3-20wt%; placing the sodium chloride aqueous solution in an electrolytic cell provided with two titanium electrodes for electrolysis to obtain the disinfectant.

[0019] Furthermore, the spraying comprises: placing the low-temperature disinfection gel in a liquid storage tank of a spraying device, spraying it on the surface of the object to be disinfected with a spray pen with a nozzle diameter of 0.1-1 mm, the spraying pressure of the spray pen is 20-30 psi; the spraying speed is 0.1-0.5 ml / s; the spraying distance between the spray pen and the surface of the object to be disinfected is maintained at 3-10 cm, and the low-temperature disinfection gel forms a coating containing a zwitterionic compound at room temperature;

[0020] Preferably, an ultraviolet lamp with an emission wavelength of 250 nm to 260 nm is used to irradiate the surface of the object to be sterilized for 1 to 10 minutes to accelerate the polymerization of the low-temperature sterilization gel to form a coating.

[0021] Beneficial effects: The low-temperature disinfection gel of the present invention contains PVA. When a PVA solution of appropriate concentration is sprayed on the surface of a low-temperature object, rapid freezing occurs, causing the water in the PVA solution to form ice crystals, which cause the PVA molecular chains to approach each other and form cross-linking points. When a subsequent sodium hypochlorite solution is sprayed, thawing occurs, the ice crystals melt, and the PVA chains melt and solidify again. After the freeze-thaw cycle, the mechanical properties of the PVA layer are enhanced, which is more conducive to the attachment of betaine and hexadecyltrimethylammonium chloride to form a molecular layer.

[0022] Furthermore, the low-temperature disinfection gel of the present invention contains betaine, and the betaine molecule contains an amino group (-NH2) and two methyl groups (-CH3) and is connected to a carboxyl group (-COOH). Betaine exhibits the characteristics of a zwitterion in aqueous solution, that is, it can carry both positive and negative charges. This characteristic comes from its chemical structure. The nitrogen atom of betaine carries a positive charge. This nitrogen atom is connected to three methyl groups to form a positively charged tetramethylammonium ion (-N(CH3)3 + The carboxylate ion (-COO - ) carries a negative charge, making the overall molecule of betaine behave as a doubly charged zwitterion (i.e., a state with both positive and negative charges) in water.

[0023] Furthermore, the low-temperature disinfection gel of the present invention can significantly improve the charge distribution on the surface of the object to be disinfected. Charge distribution is a very important property of the object to be disinfected and has a decisive influence on the interaction between the surface and bacteria. The material with a zwitterionic surface contains both cationic and anionic groups. This zwitterionic surface can resist the adsorption of proteins, thereby preventing the attachment of bacteria. This is because the zwitterionic surface with strong ionic solvation can strongly and stably bind water molecules through electrostatic action, thereby forming a hydration layer (an energy and physical barrier) to resist the adsorption of proteins; at the same time, the zwitterionic surface can minimize the electrostatic interaction between proteins / bacteria and materials.

[0024] In addition, in the B solution contained in the low-temperature disinfection gel of the present invention, 3-(cyclohexyl)aminopropanesulfonic acid is a non-toxic biochemical buffer, which mainly promotes the hydrolysis of betaine to generate carboxylate ions (-COO - ).

[0025] Further, low temperature disinfection gel of the present invention contains hexadecyl trimethyl ammonium chloride, which is a typical quaternary ammonium salt, and the nitrogen atom in the hexadecyl trimethyl ammonium chloride has a positive charge, so it belongs to a quaternary ammonium salt, and this positive charge is formed by four alkyl groups (a long chain and three methyl groups) combined by the nitrogen atom. In general, bacterial cells are wrapped by a peptidoglycan layer (composed of sugar and amino acids), which determines that most bacteria are negatively charged. Therefore, positively charged material surfaces are widely used in capturing or killing bacteria.

[0026] In summary, the low-temperature disinfection gel of the present invention forms an organic molecular film with positive and negative charges interlaced on the surface of the object to be disinfected by spraying, and the formed zwitterionic polymer surface can effectively resist the adhesion of bacteria for a long time. When in use, a disinfectant is used, such as sodium hypochlorite containing effective sodium hypochlorite, which is a strong oxidant and can play a role in rapid sterilization. At the same time, the temperature of the disinfectant is higher than the solution temperature of the low-temperature object, so that the PVA gel is gradually thawed, and re-frozen after spraying. After the freeze-thaw cycle, the mechanical properties of the PVA gel are enhanced. This is because: in the freezing stage of PVA, the ice crystals formed by water freezing push the PVA chains to approach each other, forming a rich PVA phase, separating from the water phase, and the phase separation is aggravated. As the freeze-thaw cycle continues, the polymer-rich region is further enriched by more chains, and contacts each other more closely, further facilitating the formation of hydrogen bonds. Due to the high crystallinity of PVA, these chains tend to form an ordered array, and the hydrogen bonds between molecules and within molecules lock these crystal regions together, so that the network has structural integrity. Since the movement of macromolecules is usually a slow process, not all PVA chains participate in the gelation of the first cycle. Therefore, more cycles increase the crystallinity of PVA and ensure that a larger content of PVA participates in the gel structure, thereby producing a stronger and more stable gel, further enhancing the antibacterial and anti-bacterial adhesion capabilities of the surface zwitterionic polymers, thereby achieving a long-lasting and highly effective antibacterial effect on cold chain items. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the technical solution of the present invention, the accompanying drawings will be briefly introduced below. Obviously, the accompanying drawings in the following description only relate to some embodiments of the present invention, but are not intended to limit the present invention.

[0028] Figure 1 This is a schematic diagram of the sterilization process provided by an embodiment of the present invention, wherein (a) shows an object to be sterilized carrying bacteria; (b) shows spraying disinfectant to kill some bacteria on the surface and achieve a certain cleaning purpose; (c) shows that after the PVA gel layer is formed, most bacteria are blocked from adhering, and a small part of bacteria adhere to the surface; (d) shows that the PVA gel layer undergoes a freeze-thaw cycle, further enhancing the effect of blocking bacteria from adhering. At the same time, spraying disinfectant kills a small part of the adhering bacteria. DETAILED DESCRIPTION

[0029] The preferred embodiments of the present invention will be described in more detail below. Although the preferred embodiments of the present invention are described below, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments set forth herein. In the examples, those without specifying specific techniques or conditions are carried out according to the techniques or conditions described in the literature in this area or according to the product specification. Those without specifying the manufacturer of reagents or instruments used are all conventional products that can be obtained commercially. In the following examples, if not clearly stated, "%" refers to weight percentage.

[0030] Example 1

[0031] To prepare the low temperature disinfection gel, the steps are as follows:

[0032] A 5 wt % PVA aqueous solution was prepared, which was recorded as solution A. 1 wt % betaine was dissolved in 15 mM 3-(cyclohexyl)aminopropanesulfonic acid, pH = 10.0, and stirred at 35-40° C. for 2-5 hours to cause a hydrolysis reaction to obtain solution B. A 1 wt % hexadecyltrimethylammonium chloride aqueous solution was prepared, which was recorded as solution C.

[0033] Solutions A, B and C were mixed in a ratio of 70 vol% of solution A, 15 vol% of solution B and 15 vol% of solution C, and the pH value was adjusted to 8 with 0.1 mol / L NaOH solution. The mixed solution was fully stirred to ensure that the three components were evenly dispersed to obtain solution D, which was a low-temperature disinfection gel.

[0034] To prepare the disinfectant, follow these steps:

[0035] A 10 wt% sodium chloride aqueous solution was placed in an electrolytic cell equipped with two titanium electrodes at 0.2 A / cm 2 Electrolysis was carried out at a current density of 1500 ppm to obtain disinfectant E with a hypochlorous acid concentration of 1500 ppm.

[0036] The reagent prepared above is used for low-temperature disinfection. It should be noted that low-temperature disinfection in the present invention is relative to high-temperature disinfection under a heating environment. There is no limit on the disinfection temperature, which can be room temperature or below 0 degrees Celsius. Figure 1 , the specific method is as follows:

[0037] Spray disinfectant E evenly on the surface of the object to be disinfected. Wait for about 10 minutes at room temperature and rinse thoroughly with plenty of water to kill the original bacteria on the surface.

[0038] Place solution D in a liquid storage tank and spray the mixed solution onto the surface to be disinfected using a spray pen with a nozzle diameter of about 0.5 mm. The spray pressure of the spray pen is 25 psi; the spray speed of the mixture is about 0.3 ml / s; the spray distance between the spray pen and the substrate surface is maintained at 5 cm. Wait for about 30 minutes to obtain a coating surface with a zwitterionic compound.

[0039] Spray disinfectant E evenly on the surface of the object to be disinfected again. Wait for about 10 minutes and rinse thoroughly with plenty of water.

[0040] In order to evaluate the disinfection effect of the above reagents, a bacterial detection experiment was conducted as follows:

[0041] Preparation of bacterial carrier:

[0042] Take fresh cultures of Staphylococcus aureus and Escherichia coli on nutrient agar medium, wash the bacterial lawn with phosphate buffered saline (PBS), and vibrate to make the bacteria evenly suspended. Then dilute with trypticase soy broth to prepare a bacterial count of 1×10 8 -5×10 8 cfu / mL bacterial suspension. When staining with the drop method, 10 μL of bacterial suspension was dripped on each bacterial sheet (10 mm × 10 mm), dried in a biosafety cabinet, and stored outdoors in a low-temperature test environment for 30 min before the test. Ensure that the amount of bacteria recovered from the bacterial sheet is 5×10 5 -5×10 6 cfu / piece.

[0043] Simulated on-site disinfection test:

[0044] Three groups of bacterial staining sheets were placed in an environment with a temperature of -18°C and a humidity of 55%, each group consisting of 4 bacterial staining sheets. One sheet was used for separate sampling before disinfection, one sheet was used for separate sampling after disinfection, one sheet was used for separate sampling 12 hours after disinfection, and one sheet was used for separate sampling 24 hours after disinfection. The bacterial staining sheet disinfection method here adopts the low-temperature disinfection method described in this embodiment.

[0045] Bacteria detection:

[0046] The collected bacterial slices (including the unsterilized control group bacterial slices) were placed in a test tube containing 5 mL of a neutralizer (PBS containing 3000 mg / L sodium thiosulfate + 10 g / L Tween-80 + 1000 mg / L lecithin, and verified by the neutralizer identification test), and then fully shaken and eluted. The eluate was inoculated and cultured, and the live bacteria were counted and the killing logarithm was calculated. The experiment was repeated three times.

[0047] It can be seen from Table 1 that the average killing logarithm of the disinfectant for the two bacteria immediately after disinfection, 12 hours after disinfection, and 24 hours after disinfection all reached more than 3, showing a good disinfection effect.

[0048] Table 1 Effect data of low temperature disinfection in Example 1

[0049]

[0050] Example 2

[0051] To prepare the low temperature disinfection gel, the steps are as follows:

[0052] A 3 wt % PVA aqueous solution was prepared, which was recorded as solution A. 1 wt % betaine was dissolved in 15 mM 3-(cyclohexyl)aminopropanesulfonic acid, pH = 10.0, and stirred at 35-40° C. for 2-5 hours to cause a hydrolysis reaction to obtain solution B. A 0.5 wt % hexadecyltrimethylammonium chloride aqueous solution was prepared, which was recorded as solution C.

[0053] Solutions A, B and C were mixed at a ratio of 80 vol% of solution A, 10 vol% of solution B and 10 vol% of solution C, and the pH value was adjusted to 9 with 0.1 mol / L NaOH solution. The mixed solution was fully stirred to ensure that the three components were evenly dispersed to obtain solution D, which was a low-temperature disinfection gel.

[0054] To prepare the disinfectant, follow these steps:

[0055] A 10 wt% sodium chloride aqueous solution was placed in an electrolytic cell equipped with two titanium electrodes at 0.2 A / cm 2 Electrolysis was carried out at a current density of 1500 ppm to obtain disinfectant E with a hypochlorous acid concentration of 1500 ppm.

[0056] The reagent prepared above is used for low temperature disinfection, and the specific method is as follows:

[0057] Spray disinfectant E evenly on the surface of the object to be disinfected. Wait for about 10 minutes at room temperature and rinse thoroughly with plenty of water to kill the original bacteria on the surface.

[0058] Place solution D in a liquid storage tank and spray the mixed solution onto the surface to be disinfected using a spray pen with a nozzle diameter of about 0.2 mm. The spray pressure of the spray pen is 30 psi; the spray speed of the mixture is about 0.3 ml / s; the spray distance between the spray pen and the substrate surface is maintained at 6 cm. Wait for about 30 minutes to obtain a coating surface with zwitterionic compounds.

[0059] Spray disinfectant E evenly on the surface of the object to be disinfected again. Wait for about 10 minutes and rinse thoroughly with plenty of water.

[0060] In order to evaluate the disinfection effect of the above reagents, a bacterial detection experiment was conducted, and the preparation of the bacterial carrier, the simulated on-site disinfection test and the bacterial detection were the same as those in Example 1. The disinfection method used in the simulated on-site disinfection test was the low-temperature disinfection method described in this example.

[0061] After testing, the disinfectant had an average killing logarithm of more than 3 for both bacteria immediately after disinfection, 12 hours after disinfection, and 24 hours after disinfection, indicating a good disinfection effect.

[0062] Example 3

[0063] To prepare the low temperature disinfection gel, the steps are as follows:

[0064] A 1 wt % PVA aqueous solution was prepared, which was recorded as solution A. 1 wt % betaine was dissolved in 15 mM 3-(cyclohexyl)aminopropanesulfonic acid, pH = 11.0, and stirred at 35-40° C. for 2-5 hours to cause a hydrolysis reaction to obtain solution B. A 1 wt % hexadecyltrimethylammonium chloride aqueous solution was prepared, which was recorded as solution C.

[0065] Solutions A, B and C were mixed in a ratio of 70 vol% of solution A, 15 vol% of solution B and 15 vol% of solution C, and the pH value was adjusted to 8 with 0.1 mol / L NaOH solution. The mixed solution was fully stirred to ensure that the three components were evenly dispersed to obtain solution D, which was a low-temperature disinfection gel.

[0066] To prepare the disinfectant, follow these steps:

[0067] A 10 wt% sodium chloride aqueous solution was placed in an electrolytic cell equipped with two titanium electrodes at 0.2 A / cm 2 Electrolysis was carried out at a current density of , and a disinfectant E with a hypochlorous acid concentration of 500 ppm was obtained.

[0068] The reagent prepared above is used for low temperature disinfection, and the specific method is as follows:

[0069] Spray disinfectant E evenly on the surface of the object to be disinfected. Wait for about 10 minutes at room temperature and rinse thoroughly with plenty of water to kill the original bacteria on the surface.

[0070] Place solution D in a liquid storage tank and spray the mixed solution onto the surface to be disinfected using a spray pen with a nozzle diameter of about 0.8 mm. The spray pressure of the spray pen is 20 psi; the spray speed of the mixture is about 0.3 ml / s; the spray distance between the spray pen and the substrate surface is maintained at 3 cm. Wait for about 30 minutes to obtain a coating surface with a zwitterionic compound.

[0071] Spray disinfectant E evenly on the surface of the object to be disinfected again. Wait for about 10 minutes and rinse thoroughly with plenty of water.

[0072] In order to evaluate the disinfection effect of the above reagents, a bacterial detection experiment was conducted, and the preparation of the bacterial carrier, the simulated on-site disinfection test and the bacterial detection were the same as those in Example 1. The disinfection method used in the simulated on-site disinfection test was the low-temperature disinfection method described in this example.

[0073] After testing, the disinfectant had an average killing logarithm of more than 3 for both bacteria immediately after disinfection, 12 hours after disinfection, and 24 hours after disinfection, indicating a good disinfection effect.

[0074] Example 4

[0075] To prepare the low temperature disinfection gel, the steps are as follows:

[0076] A 4 wt % PVA aqueous solution was prepared, which was recorded as solution A. 1 wt % betaine was dissolved in 20 mM 3-(cyclohexyl)aminopropanesulfonic acid, pH = 9.0, and stirred at 35-40° C. for 2-5 hours to cause a hydrolysis reaction to obtain solution B. A 1 wt % hexadecyltrimethylammonium chloride aqueous solution was prepared, which was recorded as solution C.

[0077] A, B and C solutions were mixed in a ratio of 70 vol% of A solution, 15 vol% of B solution and 15 vol% of C solution, and the pH value was adjusted to 7 with 0.1 mol / L NaOH solution. The mixed solution was fully stirred to ensure that the three components were evenly dispersed to obtain solution D, which was a low-temperature disinfection gel.

[0078] To prepare the disinfectant, follow these steps:

[0079] A 10 wt% sodium chloride aqueous solution was placed in an electrolytic cell equipped with two titanium electrodes at 0.2 A / cm 2 Electrolysis was carried out at a current density of , and a disinfectant E with a hypochlorous acid concentration of 2000 ppm was obtained.

[0080] The reagent prepared above is used for low temperature disinfection, and the specific method is as follows:

[0081] Spray disinfectant E evenly on the surface of the object to be disinfected. Wait for about 10 minutes at room temperature and rinse thoroughly with plenty of water to kill the original bacteria on the surface.

[0082] Place solution D in a liquid storage tank and spray the mixed solution onto the surface to be disinfected using a spray pen with a nozzle diameter of about 1 mm. The spray pressure of the spray pen is 25 psi; the spray speed of the mixture is about 0.2 ml / s; the spray distance between the spray pen and the substrate surface is maintained at 5 cm. Wait for about 30 minutes to obtain a coating surface with a zwitterionic compound.

[0083] Spray disinfectant E evenly on the surface of the object to be disinfected again. Wait for about 10 minutes and rinse thoroughly with plenty of water.

[0084] In order to evaluate the disinfection effect of the above reagents, a bacterial detection experiment was conducted, and the preparation of the bacterial carrier, the simulated on-site disinfection test and the bacterial detection were the same as those in Example 1. The disinfection method used in the simulated on-site disinfection test was the low-temperature disinfection method described in this example.

[0085] After testing, the disinfectant had an average killing logarithm of more than 3 for both bacteria immediately after disinfection, 12 hours after disinfection, and 24 hours after disinfection, indicating a good disinfection effect.

[0086] Example 5

[0087] To prepare the low temperature disinfection gel, the steps are as follows:

[0088] A 2 wt % PVA aqueous solution was prepared, which was recorded as solution A. 0.5 wt % betaine was dissolved in 10 mM 3-(cyclohexyl)aminopropanesulfonic acid, pH = 10.0, and stirred at 35-40° C. for 2-5 hours to cause a hydrolysis reaction to obtain solution B. A 1 wt % hexadecyltrimethylammonium chloride aqueous solution was prepared, which was recorded as solution C.

[0089] Solutions A, B and C were mixed in a ratio of 70 vol% of solution A, 15 vol% of solution B and 15 vol% of solution C, and the pH value was adjusted to 8 with 0.1 mol / L NaOH solution. The mixed solution was fully stirred to ensure that the three components were evenly dispersed to obtain solution D, which was a low-temperature disinfection gel.

[0090] To prepare the disinfectant, follow these steps:

[0091] A 10 wt% sodium chloride aqueous solution was placed in an electrolytic cell equipped with two titanium electrodes at 0.1 A / cm 2 Electrolysis was carried out at a current density of 1000 ppm to obtain disinfectant E with a hypochlorous acid concentration of 1000 ppm.

[0092] The reagent prepared above is used for low temperature disinfection, and the specific method is as follows:

[0093] Spray disinfectant E evenly on the surface of the object to be disinfected. Wait for about 10 minutes at room temperature and rinse thoroughly with plenty of water to kill the original bacteria on the surface.

[0094] Place solution D in a liquid storage tank and spray the mixed solution onto the surface to be disinfected using a spray pen with a nozzle diameter of about 0.5 mm. The spray pressure of the spray pen is 25 psi; the spray speed of the mixture is about 0.3 ml / s; the spray distance between the spray pen and the substrate surface is maintained at 5 cm. Wait for about 30 minutes to obtain a coating surface with a zwitterionic compound.

[0095] Spray the disinfectant E evenly on the surface of the object to be disinfected again. Irradiate the surface of the object to be disinfected with an ultraviolet lamp with an emission wavelength of 250nm to 260nm for 5 minutes to accelerate the polymerization of the low-temperature disinfection gel to form a coating, and then rinse thoroughly with plenty of clean water.

[0096] In order to evaluate the disinfection effect of the above reagents, a bacterial detection experiment was conducted, and the preparation of the bacterial carrier, the simulated on-site disinfection test and the bacterial detection were the same as those in Example 1. The disinfection method used in the simulated on-site disinfection test was the low-temperature disinfection method described in this example.

[0097] After testing, the disinfectant had an average killing logarithm of more than 3 for both bacteria immediately after disinfection, 12 hours after disinfection, and 24 hours after disinfection, indicating a good disinfection effect.

[0098] Comparative Example 1

[0099] This comparative example uses disinfectant to directly disinfect, as follows:

[0100] A 10 wt% sodium chloride aqueous solution was placed in an electrolytic cell equipped with two titanium electrodes at 0.2 A / cm 2 Electrolyze with a current density of 1500 ppm to obtain a disinfectant with a hypochlorous acid concentration of 1500 ppm. When using, spray the disinfectant evenly on the surface of the object to be disinfected. Wait for about 10 minutes and rinse thoroughly with plenty of water.

[0101] The preparation of the bacterial carrier and the bacterial detection were the same as those in Example 1, wherein the simulated on-site disinfection test referred to Example 1, except that the disinfection method of the bacterial sheet here adopted the low-temperature disinfection method described in this comparative example, that is, disinfection was performed directly with a disinfectant. The results are shown in Table 2.

[0102] It can be seen from Table 2 that although the average killing logarithm value of the disinfectant is greater than 3 immediately after disinfection, due to the lack of PVA gel layer coverage, the bacteria still have the ability to reproduce rapidly and have no long-term antibacterial ability.

[0103] Table 2 Effect data of disinfection with disinfectant without PVA gel layer

[0104]

[0105] Principle explanation: Disinfection with hypochlorous acid disinfectant, without the PVA gel layer, makes it very easy for bacteria to adhere and reproduce, and has no long-term antibacterial ability.

[0106] Comparative Example 2

[0107] This comparative example uses a low-temperature disinfection gel under acidic conditions for disinfection, as follows:

[0108] A 5 wt % PVA aqueous solution was prepared, which was recorded as solution A. 1 wt % betaine was dissolved in 15 mM 3-(cyclohexyl)aminopropanesulfonic acid, pH = 10.0, and stirred at 35-40° C. for 2-5 hours to cause a hydrolysis reaction to obtain solution B. A 1 wt % hexadecyltrimethylammonium chloride aqueous solution was prepared, which was recorded as solution C.

[0109] Solutions A, B, and C were mixed at a ratio of 70 vol% of solution A, 15 vol% of solution B, and 15 vol% of solution C, and the pH value was adjusted to 6 with 0.1 mol / L HCl solution. The mixed solution was stirred thoroughly to ensure that the three components were evenly dispersed to obtain solution D.

[0110] A 10 wt% sodium chloride aqueous solution was placed in an electrolytic cell equipped with two titanium electrodes at 0.2 A / cm 2 Electrolysis was carried out at a current density of 1500 ppm to obtain disinfectant E with a hypochlorous acid concentration of 1500 ppm.

[0111] The preparation of the bacteria-contaminated carrier, the simulated on-site disinfection test and the bacteria detection were the same as those in Example 1. The results are shown in Table 3.

[0112] As can be seen from Table 3, the average killing logarithm of the disinfectant for both bacteria is greater than 3 just after disinfection, but there are significant differences at 12h and 24h. For Escherichia coli, the average killing logarithm after 24h reaches 6.40, showing good disinfection ability for Escherichia coli. However, for Staphylococcus aureus, the average killing logarithm after 24h is only 0.98, and there is no long-term antibacterial ability against Staphylococcus aureus. This is because Staphylococcus aureus is a typical Gram-positive bacterium. If the disinfectant is acidic, it will inhibit the hydrolysis of the carboxyl group in betaine, resulting in a reduction of -COO-, and the gel layer is acidic, with no anti-Gram-positive bacteria ability.

[0113] Table 3 Effect data of disinfection by acidic PVA gel layer disinfectant

[0114]

[0115] Principle explanation: If the PVA gel layer is acidic during preparation, it will inhibit the hydrolysis of the carboxyl group in the betaine in the solution, and the -COO - The content is reduced and it has no ability to resist Gram-positive bacteria (such as Staphylococcus aureus).

[0116] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0117] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

[0118] In addition, various embodiments of the present invention may be arbitrarily combined, and as long as they do not violate the concept of the present invention, they should also be regarded as the contents disclosed by the present invention.

Claims

1. A low-temperature disinfection gel, characterized in that: The invention comprises the following components in volume percentage: 70-80% of solution A, 10-15% of solution B, and 10-15% of solution C, wherein the solution A is an aqueous solution of polyvinyl alcohol, the solution B is a solution formed by hydrolysis of betaine dissolved in an aqueous solution of 3-(cyclohexyl)aminopropanesulfonic acid, and the solution C is an aqueous solution of hexadecyltrimethylammonium chloride.

2. The low temperature disinfection gel according to claim 1, characterized in that: The concentration of polyvinyl alcohol in the A solution is 1-5 wt %.

3. The low temperature disinfection gel according to claim 1, characterized in that: The preparation method of the B solution is as follows: dissolving 0.5-1 wt% betaine in a 3-(cyclohexyl)aminopropanesulfonic acid solution containing 10-20 mM and pH=9-11, stirring at 35-40° C. for 2-5 hours to cause a hydrolysis reaction, thereby obtaining the B solution.

4. The low temperature disinfection gel according to claim 1, characterized in that: The concentration of hexadecyltrimethylammonium chloride in the C solution is 0.5-1 wt %.

5. The low-temperature disinfection gel according to any one of claims 1 to 4, characterized in that: The low-temperature disinfection gel consists of the A solution, the B solution, the C solution, and a pH regulator.

6. The low temperature disinfection gel according to claim 5, characterized in that: The pH of the low-temperature disinfection gel is 7-9.

7. Use of the low-temperature disinfection gel according to any one of claims 1 to 6 in the disinfection of cold chain products.

8. A method for low-temperature disinfection, using the low-temperature disinfection gel according to any one of claims 1 to 6, characterized in that: The following steps are involved: S1. Use disinfectant to disinfect the items to be disinfected; S2, spraying the low-temperature disinfection gel on the surface of the disinfected object to be disinfected, so that the low-temperature disinfection gel forms a coating on the surface of the object to be disinfected; S3, disinfecting the coated article again with the disinfectant.

9. The method for low temperature sterilization according to claim 8, characterized in that: The disinfectant contains sodium hypochlorite. Preferably, the concentration of sodium hypochlorite in the disinfectant is 100-3000 ppm. More preferably, the disinfectant is prepared according to the following method: preparing a sodium chloride aqueous solution with a concentration of 3-20wt%; placing the sodium chloride aqueous solution into an electrolytic cell provided with two titanium electrodes for electrolysis to obtain the disinfectant.

10. The low temperature sterilization method according to claim 8 or 9, characterized in that: The spraying comprises: placing the low-temperature disinfection gel in a liquid storage tank of a spraying device, spraying it on the surface of the object to be disinfected with a spray pen with a nozzle diameter of 0.1-1 mm, the spraying pressure of the spray pen is 20-30 psi; the spraying speed is 0.1-0.5 ml / s; the spraying distance between the spray pen and the surface of the object to be disinfected is maintained at 3-10 cm, and the low-temperature disinfection gel forms a coating containing a zwitterionic compound at room temperature; Preferably, an ultraviolet lamp with an emission wavelength of 250 nm to 260 nm is used to irradiate the surface of the object to be sterilized for 1 to 10 minutes to accelerate the polymerization of the low-temperature sterilization gel to form a coating.

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