Liquid storage assembly for sample pretreatment device, sample pretreatment device and use method of sample pretreatment device

Abstract

The invention discloses a liquid storage assembly for a sample pretreatment device, the sample pretreatment device and a use method of the sample pretreatment device. The liquid storage assembly for the sample pretreatment device comprises a shell used for storing reaction liquid and a liquid outlet formed in the shell, and a magnetic switch piece is arranged in the shell; and the magnetic switch piece is provided with a first position for blocking the liquid outlet under the action of self gravity and a second position for unblocking the liquid outlet under the action of external magnetic force. The liquid storage assembly for the sample pretreatment device can pre-store reaction liquid required to be used, and the magnetic switch piece is used for controlling the opening and closing of the liquid outlet, so that subsequent mixing with a cell sample is facilitated. According to the pre-sample treatment device disclosed by the invention, the liquid storage assembly and the reaction chip are matched for use, and the reaction liquid in the shell can be controlled to enter the reaction cavity according to requirements, so that the mixing convenience of all liquids is improved, and the working efficiency is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of cell detection, and in particular relates to a liquid storage component for a sample pre-treatment device, a sample pre-treatment device and a use method thereof. Background Art

[0002] In traditional flow cytometry, cells are usually required to undergo a series of pre-treatments, which usually involve relatively complex operations, including cell lysis, separation, centrifugation, staining, and room temperature light-protected reactions.

[0003] When performing a series of pre-treatments on cells, first, the required cell samples, antibodies, and lysate are divided into several centrifuge tubes, then the centrifuge tube containing the cell sample is taken out and the lysate is added, and after waiting for a while, the antibody stain is added, and incubated at room temperature in the dark. After the reaction is completed, another antibody is added, and then incubated at room temperature in the dark until all the required antibodies are added and the reaction is completed. The above steps involve multiple liquid transfer operations, which are cumbersome and require a lot of time to handle, which is time-consuming and labor-intensive.

[0004] Therefore, in view of the above technical problems, it is necessary to provide a liquid storage component for a sample pre-treatment device, a sample pre-treatment device and a method for using the same. Summary of the invention

[0005] The object of the present invention is to provide a liquid storage component for a sample pre-treatment device, a sample pre-treatment device and a method of using the same, which can solve the problem that the cell sample pre-treatment steps are multiple and complicated, resulting in low treatment efficiency.

[0006] In order to achieve the above object, a technical solution provided by a specific embodiment of the present invention is as follows:

[0007] In a first aspect, a specific embodiment of the present invention provides a liquid storage component for a sample pretreatment device, comprising a shell for storing a reaction liquid and a liquid outlet arranged on the shell, a magnetic switch component is arranged in the shell, and the magnetic switch component has a first position for sealing the liquid outlet under the action of its own gravity, and a second position for unsealing the liquid outlet under the action of an external magnetic force.

[0008] In one or more embodiments of the present invention, the inner wall of the shell includes a switch member guide surface, the liquid outlet is opened on the switch member guide surface, and the magnetic switch member can slide along the switch member guide surface to the first position under the action of its own gravity.

[0009] In one or more embodiments of the present invention, the shell includes a protruding portion protruding outward, and when the magnetic switch element is in the first position, the outer wall of the magnetic switch element and the inner wall of the shell at the protruding portion abut against each other without any gap.

[0010] In one or more embodiments of the present invention, the liquid storage assembly for the sample pre-treatment device further comprises a liquid outlet pipe connected to the liquid outlet, and a spike portion is provided at one end of the liquid outlet pipe away from the shell;

[0011] And / or, the shell is provided with a liquid inlet for inputting the reaction liquid, and the liquid inlet can be externally connected to a positive pressure device for applying positive pressure to the reaction liquid in the shell.

[0012] In a second aspect, a specific embodiment of the present invention provides a sample pre-treatment device, comprising a reaction chip and a liquid storage component that are arranged in a coordinated manner, wherein the liquid storage component is the liquid storage component for the sample pre-treatment device described above;

[0013] The reaction chip is provided with a reaction chamber and at least two flow channels connected to the reaction chamber, and each of the flow channels is provided with an access port at one end away from the reaction chamber;

[0014] The liquid outlet of the shell can be matched with the inlet, and the magnetic switch element can be controlled to move to a first position or a second position to control the reaction liquid of the shell to flow into the flow channel.

[0015] In one or more embodiments of the present invention, the reaction chip is provided with a liquid collection chamber for outputting the mixed liquid, the liquid collection chamber is communicated with the reaction chamber, and the liquid collection chamber can be externally connected to a negative pressure device for taking out the reaction liquid.

[0016] In a third aspect, a specific embodiment of the present invention provides a method for using the above-mentioned sample pre-treatment device, comprising the following steps:

[0017] driving the magnetic switch element to move to a first position and injecting a reaction liquid into the housing;

[0018] forming a communication port communicating with the reaction chamber of the reaction chip, and injecting a cell sample into the reaction chamber through the communication port;

[0019] The shells filled with different reaction liquids are matched and installed to the corresponding access ports on the reaction chip, and the corresponding magnetic switch components are controlled to move to the second position, so that the reaction liquids enter the reaction chamber through the flow channel.

[0020] In one or more embodiments of the present invention, controlling the corresponding magnetic switch element to move to the second position specifically includes:

[0021] Each shell is configured with an electromagnet, and based on the cell fluid production requirements, the switching time of each electromagnet is controlled by a control circuit;

[0022] And / or, before driving the magnetic switch element to move to the first position, the liquid outlet of the housing is sealed in advance with a sealing film.

[0023] In one or more embodiments of the present invention, forming a communication port communicating with the reaction chamber specifically includes:

[0024] The communication port is formed by removing the sealing film pre-set on the reaction chamber; or, the sealing film pre-set on the reaction chamber is pierced to form the communication port.

[0025] In one or more embodiments of the present invention, the method for using the sample pre-treatment device further includes: applying positive pressure to the housing by a positive pressure device to drive the reaction liquid to enter the reaction chamber through the flow channel;

[0026] And / or, negative pressure is applied to the reaction chip by a negative pressure device to remove the mixed sample from the reaction chip.

[0027] Compared with the prior art, the sample pre-treatment device of the present invention can store the required reaction liquid in advance with the liquid storage component, and use the magnetic switch to control the switch of the liquid outlet, so as to facilitate the subsequent mixing with the cell sample. The sample pre-treatment device can control the reaction liquid in the shell to enter the reaction chamber as required through the coordinated use of the liquid storage component and the reaction chip, thereby improving the convenience of mixing the various liquids and improving work efficiency. Since multiple pipetting steps are not required, the mixing of different liquids can be completed by controlling the automatic switch of the magnetic switch, thereby improving work efficiency. At the same time, compared with the existing automated working equipment, which is bulky and expensive, the sample pre-treatment device of the present invention has the advantages of small size and low cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0029] Figure 1 This is a schematic structural diagram of a liquid storage assembly for a sample pre-treatment device according to an embodiment of the present invention;

[0030] Figure 2 It is a cross-sectional schematic diagram of a liquid storage assembly for a sample pre-treatment device in one embodiment of the present invention;

[0031] Figure 3 This is a schematic structural diagram of a sample pre-treatment device in one embodiment of the present invention;

[0032] Figure 4 Schematic diagram of an explosion of a reaction chip of a sample pre-treatment device in one embodiment of the present invention.

[0033] Description of main reference numerals:

[0034] 100, liquid storage component; 110, shell; 111, liquid outlet; 112, liquid inlet; 113, protrusion; 120, magnetic switch; 130, liquid outlet pipe; 131, spike; 200, reaction chip; 210, reaction chamber; 220, flow channel; 230, access port; 240, liquid extraction chamber. DETAILED DESCRIPTION

[0035] In order to enable those skilled in the art to better understand the technical solutions in the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0036] Reference Figure 1 and Figure 2 In one embodiment of the present invention, a liquid storage component for a sample pretreatment device is provided, including a shell 110 for storing a reaction liquid and a liquid outlet 111 arranged on the shell 110, a magnetic switch component 120 is arranged in the shell 110, and the magnetic switch component 120 has a first position for blocking the liquid outlet 111 under the action of its own gravity, and a second position for unblocking the liquid outlet 111 under the action of an external magnetic force.

[0037] When the magnetic switch 120 is driven to move to the first position and the reaction liquid is loaded into the housing 110, the reaction liquid can be stored in advance for subsequent use. During subsequent mixing, the magnetic switch 120 is driven to move to the second position, so that the reaction liquid in the housing 110 can flow out and mix with the cell sample. In this embodiment, the magnetic switch 120 can be driven to move to the second position by a magnet or an electromagnet.

[0038] Reference Figure 2 The inner wall of the housing 110 includes a switch member guide surface, and the liquid outlet 111 is provided on the switch member guide surface. The magnetic switch member 120 can slide to the first position along the switch member guide surface under the action of its own gravity. By providing the switch member guide surface, the magnetic switch member 120 can be effectively guided to move to the first position to block the liquid outlet 111.

[0039] Reference Figure 1 and Figure 2, the housing 110 includes a protruding portion 113 protruding outward, and when the magnetic switch component 120 is in the first position, the outer wall of the magnetic switch component 120 and the inner wall of the housing 110 at the protruding portion 113 abut against each other without a gap. Therefore, by reasonably setting the shape of the outer wall of the magnetic switch component 120 and the inner wall of the protruding portion 113, the liquid outlet 111 can be effectively blocked. In this embodiment, the protruding portion 113 is cone-shaped, the switch component guide surface is arranged on the inner wall of the housing 110 corresponding to the protruding portion 113, and the magnetic switch component 120 is a sphere, which can effectively ensure that the magnetic switch component 120 blocks the liquid outlet 111. In other embodiments, the protruding portion 113 can also be a sphere or a square body, etc., and the outer wall of the magnetic switch component 120 can be designed accordingly.

[0040] Reference Figure 3 and Figure 4 In one embodiment of the present invention, a sample pre-treatment device is also provided, comprising a reaction chip 200 and a liquid storage component 100 which are arranged in cooperation, and the liquid storage component 100 is the liquid storage component for the sample pre-treatment device mentioned above. The reaction chip 200 is provided with a reaction chamber 210 and at least two flow channels 220 which are connected with the reaction chamber 210, and each flow channel 220 is provided with an access port 230 at one end away from the reaction chamber 210; the liquid outlet 111 of the housing 110 can cooperate with the access port 230, and the magnetic switch 120 can be controlled to move to the first position or the second position to control the reaction liquid of the housing 110 to enter the flow channel 220.

[0041] In this embodiment, three flow channels 220 are provided for mixing three reaction solutions with cell samples. In other embodiments, a corresponding number of flow channels 220 can be designed according to the number of reaction solutions required. Among them, according to different cell solution production requirements, the reaction solution can be a lysate, an antibody or a dye solution, etc.

[0042] When using the sample pre-treatment device of this embodiment, the reaction liquid is pre-loaded into the housing 110, and then the liquid outlet 111 of the housing 110 is matched with the access port 230 of the reaction chip 200, and the magnetic switch 120 is controlled to move so that the reaction liquid enters the reaction chamber 210 through the flow channel 220, and finally the obtained cell fluid is placed in the cell detector to complete the cell detection. The coordinated use of the liquid storage component 100 and the reaction chip 200 enables the reaction liquid in the housing 110 to enter the reaction chamber 210 as required, thereby improving the convenience of mixing the cell fluid.

[0043] Specifically, refer to Figure 4In this embodiment, the reaction chip 200 includes two layers, the lower layer is etched with the above-mentioned flow channel 220 and reaction chamber 210. The upper layer is a cover plate, which is provided with the above-mentioned access port 230 connected to the flow channel 220. The reaction chip 200 is set in a plate shape, which is convenient for expanding more flow channels 220 to adapt to the mixing work of multiple reaction solutions.

[0044] Reference Figure 2 Further, the liquid storage assembly for the sample pre-treatment device further includes a liquid outlet pipe 130 connected to the liquid outlet 111, and a spike portion 131 is provided at one end of the liquid outlet pipe 130 away from the housing 110. The spike portion 131 improves the convenience of the liquid outlet 111 being inserted into the access port 230.

[0045] Reference Figure 2 and Figure 4 In this embodiment, the shell 110 is provided with a liquid inlet 112 for inputting the reaction liquid, and the liquid inlet 112 can be connected to a positive pressure device to apply positive pressure to the reaction liquid in the shell 110. The reaction chip 200 is provided with a liquid extraction chamber 240 for outputting the mixed liquid, and the liquid extraction chamber 240 is connected to the reaction chamber 210, and the liquid extraction chamber 240 can be connected to a negative pressure device to extract the reaction liquid. The positive pressure device can speed up the flow rate of the reaction liquid in the flow channel 220, and the negative pressure device facilitates the removal of the mixed sample from the liquid extraction chamber 240. In this embodiment, the plate-shaped reaction chip 200 can provide a flat surface for positive pressure or negative pressure, so as to facilitate the connection of positive pressure devices and negative pressure devices.

[0046] Since the reaction solution cannot usually be stored in large quantities at room temperature, in fact, antibodies and lysates are usually frozen in refrigerators in large quantities, and need to be taken out and thawed before being packaged for use. Therefore, compared to the structure in which the cell sample and the reaction solution are placed in different liquid bottles respectively, and the reaction solution is sucked into the liquid bottle containing the cell sample through pipelines and pumps, since this structure requires that the reaction solution be exposed to room temperature for a long time, it cannot be applied to cell flow sample pre-treatment, and the sample pre-treatment device of the present embodiment can effectively solve the above problems. At the same time, the above structure is used to replace the pipeline and the pump. The operation steps are cumbersome, and the sample pre-treatment device of the present embodiment is disposable, eliminating the connection and purchase of the pipeline and the pump, and is therefore faster and more convenient. Therefore, the sample pre-treatment device of the present embodiment has the advantages of small size, convenient operation, and low production cost.

[0047] Reference Figure 3 In one embodiment of the present invention, there is also provided a method for using the sample pre-treatment device, comprising the following steps:

[0048] S1. Drive the magnetic switch element 120 to move to the first position, and inject the reaction liquid into the housing 110. In this embodiment, by adjusting the placement position of the housing 110, the magnetic switch element can be moved to the first position under the action of gravity.

[0049] S2. Form a communication port connected to the reaction chamber 210 of the reaction chip 200, and inject the cell sample into the reaction chamber 210 through the communication port. In this embodiment, a sealing film is pre-set on the reaction chamber 210 of the reaction chip 200. The sealing film pre-set on the reaction chamber 210 can be removed to form the communication port; or the sealing film pre-set on the reaction chamber 210 can be punctured to form the communication port.

[0050] S3, the housing 110 filled with different reaction liquids is installed in cooperation with the corresponding access port 230 on the reaction chip 200, and the corresponding magnetic switch 120 is controlled to move to the second position, so that the reaction liquid enters the reaction chamber 210 through the flow channel 220.

[0051] The control of the corresponding magnetic switch 120 to move to the second position at a fixed time specifically includes: configuring an electromagnet for each housing 110, and controlling the switching time of each electromagnet through a control circuit based on the cell solution production requirements. When the reaction solution in one housing 110 is fully mixed and reacted with the cell sample in the reaction chamber 210, the next electromagnet can be driven to turn on to perform the mixing reaction of the next reaction solution.

[0052] It can be understood that in this embodiment, the control circuit controls the opening time of the electromagnet, which can improve the convenience of the cell fluid sample pre-processing operation. That is, the operator only needs to preset the opening time of each electromagnet, and the sample pre-processing device can automatically complete the preparation of the cell fluid mixed sample. Since the reaction process does not require the intervention of relevant personnel again, it can save a lot of manpower and material resources.

[0053] In an optional embodiment, in order to speed up the reaction liquid in the housing 110 entering the reaction chip 200, a positive pressure device is used to apply positive pressure to the housing 110 to drive the reaction liquid through the flow channel 220 into the reaction chamber 210. At the same time, in order to facilitate the removal of the cell mixed sample, in an optional embodiment, a negative pressure device can also be used to apply negative pressure to the reaction chip 200 to remove the mixed sample from the reaction chip 200.

[0054] In an optional embodiment, before the magnetic switch 120 is driven to move to the first position, the liquid outlet 111 of the housing 110 is sealed in advance by a sealing film. The sealing film can further ensure that the liquid loaded into the housing 110 will not leak from the liquid outlet 111.

[0055] Compared with the traditional method of placing the cell sample in a sample tube first and then adding an appropriate amount of reaction solution to the sample tube in turn, this embodiment reduces multiple pipetting steps by using a sample pre-treatment device, and only needs to control the automatic switching time of the magnetic switch 120 to complete the flow pre-treatment of the cell solution, and the treatment process does not require the intervention of relevant personnel, thereby improving work efficiency.

[0056] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

[0057] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A liquid storage assembly for a sample pre-treatment device, characterized in that: The invention comprises a shell (110) for storing a reaction liquid and a liquid outlet (111) arranged on the shell (110); a magnetic switch component (120) is arranged in the shell (110); the magnetic switch component (120) has a first position for sealing the liquid outlet (111) under the action of its own gravity, and a second position for unsealing the liquid outlet (111) under the action of an external magnetic force.

2. The liquid storage assembly for a sample pre-treatment device according to claim 1, characterized in that: The inner wall of the housing (110) comprises a switch member guide surface, the liquid outlet (111) is opened on the switch member guide surface, and the magnetic switch member (120) can slide along the switch member guide surface to the first position under the action of its own gravity.

3. The liquid storage assembly for a sample pre-treatment device according to claim 2, characterized in that: The housing (110) comprises a protruding portion (113) protruding outward, and when the magnetic switch component (120) is located at a first position, an outer wall of the magnetic switch component (120) and an inner wall of the housing (110) at the protruding portion (113) abut against each other without any gap.

4. The liquid storage assembly for a sample pre-treatment device according to claim 2, characterized in that: It also includes a liquid outlet pipe (130) connected to the liquid outlet (111), and a spike portion (131) is provided at one end of the liquid outlet pipe (130) away from the housing (110); And / or, the shell (110) is provided with a liquid inlet (112) for inputting the reaction liquid, and the liquid inlet (112) can be externally connected to a positive pressure device for applying positive pressure to the reaction liquid in the shell (110).

5. A sample pre-treatment device, characterized in that: It comprises a reaction chip (200) and a liquid storage component (100) which are arranged in a coordinated manner, wherein the liquid storage component (100) is a liquid storage component for a sample pre-treatment device according to any one of claims 1 to 4; The reaction chip (200) is provided with a reaction chamber (210) and at least two flow channels (220) connected to the reaction chamber (210), and each of the flow channels (220) is provided with an access port (230) at one end away from the reaction chamber (210); The liquid outlet (111) of the housing (110) can cooperate with the inlet (230), and the magnetic switch element (120) can be controlled to move to a first position or a second position to control the reaction liquid in the housing (110) to flow into the flow channel (220).

6. The sample pre-treatment device according to claim 5, characterized in that: The reaction chip (200) is provided with a liquid extraction chamber (240) for outputting the mixed liquid, the liquid extraction chamber (240) is communicated with the reaction chamber (210), and the liquid extraction chamber (240) can be externally connected to a negative pressure device for extracting the reaction liquid.

7. A method for using the sample pre-treatment device according to any one of claims 5 to 6, characterized in that: The following steps are involved: driving the magnetic switch element (120) to move to a first position, and injecting a reaction liquid into the housing (110); forming a communication port communicating with the reaction chamber (210) of the reaction chip (200), and injecting a cell sample into the reaction chamber (210) through the communication port; The housing (110) containing different reaction liquids is mounted on the corresponding access port (230) on the reaction chip (200), and the corresponding magnetic switch (120) is controlled to move to a second position so that the reaction liquid enters the reaction chamber (210) through the flow channel (220).

8. The method for using the sample pre-treatment device according to claim 7, characterized in that: Controlling the corresponding magnetic switch element (120) to move to a second position specifically comprises: An electromagnet is correspondingly configured for each housing (110), and based on the cell fluid production requirements, the switching time of each electromagnet is controlled by a control circuit; And / or, before driving the magnetic switch element (120) to move to the first position, the liquid outlet (111) of the housing (110) is sealed in advance with a sealing film.

9. The method for using the sample pre-treatment device according to claim 7, characterized in that: Forming a communication port communicating with the reaction chamber (210), specifically comprising: Removing the sealing film pre-set on the reaction chamber (210) to form the communication port; Alternatively, a sealing film pre-set on the reaction chamber (210) is punctured to form the communication port.

10. The method for using the sample pre-treatment device according to claim 7, characterized in that: Also includes: Applying positive pressure to the housing (110) through a positive pressure device to drive the reaction liquid to enter the reaction chamber (210) through the flow channel (220); And / or, negative pressure is applied to the reaction chip (200) by a negative pressure device to remove the mixed sample from the reaction chip (200).

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