On-orbit microorganism detection device

Abstract

The invention provides an on-orbit microorganism detection device. The on-orbit microorganism detection device comprises a detection outer box, a chip clamping box inserted into the detection outer box and an optical fiber connecting assembly used for connecting the detection outer box and the chip clamping box through optical fibers. The chip card box comprises a card box body, a micro-cavity optical fiber detection chip and a perfusion module, and the perfusion module is connected with the micro-cavity optical fiber detection chip; the detection outer box comprises an outer box body, a detection module, a control module and a power supply module, the detection module is in optical fiber connection with the microcavity optical fiber detection chip, and the control module is electrically connected with the detection module, the perfusion module and the power supply module; according to the invention, the chip card box provided with the micro-cavity optical fiber detection chip and the multi-channel fluorescence detection chip is arranged, so that high-sensitivity, rapid and label-free parallel detection can be carried out on a to-be-detected space microorganism sample; instruction communication and data contact with the outside can be completed through the detection outer box, efficient detection of on-orbit microorganisms is achieved, and technical support is provided for on-orbit microorganism detection.

Description

Technical Field

[0001] The present invention relates to the field of biomedical detection, and in particular to an on-orbit microorganism detection device. Background Art

[0002] With the construction and operation of the Chinese space station, the issue of microbial safety control in the manned sealed cabin has become increasingly prominent. As Earth microorganisms inevitably enter the space station with humans and cargo, the cabin environment becomes complex and the microbial population structure changes accordingly. The microbial species in the International Space Station change due to different countries, different launch sites, and different crews. Space radiation and microgravity environment break the original bacterial community structure and form a new one.

[0003] In order to control the level of microorganisms in the sealed cabin, it is necessary to conduct real-time, rapid and highly sensitive detection of water, air and surface microorganisms in orbit, so as to promptly inform astronauts of the microbial safety threats in their environment and guide the disinfection of microorganisms. In addition, through continuous sampling, dynamic detection of changes in the structure of microbial communities in the space station, succession laws and biosafety risk assessment, the separation of harmful and difficult-to-cultivate microorganisms is carried out.

[0004] At present, the technology of microbial detection methods in the space station is relatively backward. The method based on nucleic acid amplification has the disadvantages of long detection time and high detection limit; the method based on culture cannot truly reflect the number of all microorganisms and there is a risk of secondary contamination; the method based on physiological and biochemical reactions has low detection sensitivity and cannot identify the classification status of microorganisms. Due to the small number of microorganisms, low concentration and high mutation rate in the space environment, an on-orbit microbial detection device with high sensitivity and rapid detection and identification of space environment microorganisms is needed to achieve timely warning and prevention and control of very few harmful microorganisms. Summary of the invention

[0005] In order to overcome the defects of the above-mentioned prior art, the present invention provides an on-orbit microorganism detection device to solve the defects of traditional detection methods and has the ability to detect and identify microorganisms in space environment with high sensitivity and rapidity.

[0006] In order to achieve the above technical objectives, the following technical solutions are adopted:

[0007] An on-orbit microorganism detection device comprises a detection outer box, a chip card box inserted into the detection outer box, and an optical fiber connection assembly for connecting the detection outer box and the chip card box with an optical fiber;

[0008] Chip card box includes:

[0009] Card box body;

[0010] Microcavity fiber optic detection chip, used to store microorganisms for detection;

[0011] A perfusion module is connected to the microcavity optical fiber detection chip and is used to deliver culture medium and microorganisms to the microcavity optical fiber detection chip;

[0012] The inspection box includes:

[0013] Outer box body;

[0014] A detection module is connected to the microcavity optical fiber detection chip optical fiber to detect microorganisms in the microcavity optical fiber detection chip;

[0015] The control module is electrically connected to the detection module to receive the microorganism detection result; the control module is also electrically connected to the perfusion module to control the perfusion module;

[0016] A power module, electrically connected to the control module, for providing power supply;

[0017] The microcavity optical fiber detection chip and the perfusion module are both arranged inside the card box body; the detection module, the control module and the power supply module are all arranged inside the outer box body.

[0018] Optionally, the detection module includes an excitation light source, a splitter and a plurality of photoelectric detection sensors;

[0019] The output end of the excitation light source is optically connected to the input end of the splitter, and the output end of the splitter is optically connected to the input end of the microcavity fiber detection chip; the output end of the microcavity fiber detection chip is optically connected to the input ends of multiple photoelectric detection sensors, and the output ends of the multiple photoelectric detection sensors are electrically connected to the input end of the control module.

[0020] Optionally, the microcavity optical fiber detection chip comprises a chip housing, and the chip housing is provided with a liquid inlet, a liquid outlet, a liquid inlet flow channel, a liquid outlet flow channel and a plurality of microcavities;

[0021] The liquid inlet end of the liquid inlet channel is connected to the liquid outlet end of the perfusion module through the liquid inlet port; the liquid outlet ends of the liquid inlet channel are respectively connected to the liquid inlet ends of the plurality of microcavities; the liquid outlet ends of the plurality of microcavities are respectively connected to the liquid inlet ends of the liquid outlet channel, and the liquid outlet end of the liquid outlet channel is connected to the liquid inlet end of the liquid outlet port;

[0022] The plurality of microcavities are communicated with the optical fiber connection components respectively.

[0023] Optionally, the chip card box further includes a multi-channel fluorescence detection chip;

[0024] The multi-channel fluorescence detection chip includes a sample chamber, a lysis chamber, a liquid separation valve, a negative pressure pump and a plurality of reaction chambers;

[0025] The liquid outlet of the sample chamber is connected to the liquid inlet of the negative pressure pump, and the liquid outlet of the negative pressure pump is connected to the liquid inlet of the lysis chamber; the liquid outlet of the lysis chamber is connected to the liquid inlet of the liquid separation valve, and the liquid outlet of the liquid separation valve is respectively connected to the liquid inlet of a plurality of reaction chambers;

[0026] The reaction chambers are all pre-buried with reaction reagents for microbial fluorescence detection.

[0027] Optionally, the perfusion module includes a micro pump and a solenoid valve; the liquid outlet of the solenoid valve is connected to the liquid inlet of the microcavity optical fiber detection chip, and the liquid outlet of the microcavity optical fiber detection chip is connected to the liquid inlet of the micro pump;

[0028] The card box is provided with at least two sample inlets and at least one sample outlet; the liquid outlet ends of the at least two sample inlets are connected to the liquid inlet end of the electromagnetic valve; the liquid inlet end of the at least one sample outlet is connected to the liquid outlet end of the micro pump.

[0029] Optionally, the optical fiber connection assembly includes a first optical fiber connector, a second optical fiber connector, a third optical fiber connector, a first optical fiber interface, and a second optical fiber interface;

[0030] The first end of the first optical fiber connector is connected to the output end of the splitter, and the second end of the first optical fiber connector is connected to the input end of the photoelectric detection sensor; the first end of the second optical fiber connector is connected to the third end of the first optical fiber connector through the first optical fiber interface, and the second end of the second optical fiber connector is connected to the first end of the third optical fiber connector through the second optical fiber interface; the second end and the third end of the third optical fiber connector are both connected to the microcavity optical fiber detection chip;

[0031] The first optical fiber interface is arranged on the top surface of the outer box body, and the third optical fiber interface is arranged on the top surface of the card box body.

[0032] Optionally, the detection outer box further comprises an observation module disposed inside the outer box body; the observation module comprises a single-axis slide rail, a slider, a slide rail motor, a detection lens and a signal processing circuit board;

[0033] The single-axis slide rail is fixedly installed on the inner side wall of the outer box body, the slider is slidably arranged on the slide rail, and the detection lens is fixedly arranged at the bottom of the slider; the slide rail motor is fixedly arranged at the end of the single-axis slide rail, and is used to drive the slider to drive the detection lens to move back and forth along the slide rail; the input end of the signal processing circuit board is electrically connected to the output end of the detection lens, and the output end of the signal processing circuit board is electrically connected to the input end of the control module;

[0034] A detection window is provided on the inner wall of the card box body at a position corresponding to the microcavity reaction chamber, so as to perform fluorescence detection on the microcavity reaction chamber through a detection lens.

[0035] Optionally, a control interface is provided at the bottom of the cartridge body, the input end of the control interface is electrically connected to the output end of the control module, and the output end of the control interface is electrically connected to the input ends of the perfusion module, the liquid separation valve and the negative pressure pump respectively.

[0036] Optionally, the control module includes an integrated circuit board, the input end of the integrated circuit board is electrically connected to the output end of the power module and the detection module respectively, and the output end of the integrated circuit board is electrically connected to the input end of the perfusion module;

[0037] The integrated circuit board is arranged at the bottom of the outer box body, and at least two positioning holes are opened on the integrated circuit board; the card box body is provided with at least two positioning posts corresponding to the positions of the positioning holes, and the positioning posts are inserted into the corresponding positioning holes to fix the chip card box.

[0038] Optionally, the detection outer box also includes a power supply interface, a telemetry interface and a communication interface.

[0039] The beneficial effects brought by the embodiments provided by the present invention include:

[0040] The present invention can realize high-sensitivity, rapid and label-free parallel detection of space microbial samples to be tested by providing a chip card box with a microcavity optical fiber detection chip and a multi-channel fluorescence detection chip; the detection outer box in the present invention can not only complete the injection of samples into the chip card box and the collection of detection data, but also complete command communication and data communication with the outside, thereby realizing efficient detection of on-orbit microorganisms, significantly improving detection efficiency, reducing dependence on ground support, and providing effective technical support for on-orbit microbial detection.

[0041] The microcavity fiber optic detection chip of the present invention adopts an independent layered design of the laser light path and the sample liquid path, thereby avoiding mutual interference between the laser light path and the sample liquid path, preventing mutual cutting of the path channels, improving the stability and reliability of the microcavity fiber optic detection chip, and improving the accuracy of microbial detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0043] Figure 1 A schematic diagram of the three-dimensional structure of a microorganism detection device according to an embodiment of the present specification is shown;

[0044] Figure 2A schematic diagram of the three-dimensional structure of a chip card box according to an embodiment of the present specification is shown;

[0045] Figure 3 A schematic diagram of the internal structure of a chip card box according to an embodiment of the present specification is shown;

[0046] Figure 4 A schematic diagram of the structure of a detection module according to an embodiment of this specification is shown;

[0047] Figure 5 A detection flow chart of a detection module according to an embodiment of this specification is shown;

[0048] Figure 6 A schematic diagram of wiring of a microcavity optical fiber detection chip according to an embodiment of this specification is shown;

[0049] Figure 7 A schematic diagram of the internal structure of a microcavity optical fiber detection chip according to an embodiment of the present specification is shown;

[0050] Figure 8 A schematic diagram of the structure of an observation module according to an embodiment of the present specification is shown;

[0051] Fig. 9 A schematic diagram of the internal structure of a microorganism detection device according to an embodiment of the present specification is shown;

[0052] Fig.10 A schematic diagram of the structure of a detection window in a chip card box according to an embodiment of the present specification is shown;

[0053] Fig.11 A schematic diagram showing the connection between the chip card box and the detection outer box according to an embodiment of the present specification is shown;

[0054] Among them, 1 is a detection outer box; 2 is a chip card box; 3 is a microcavity optical fiber detection chip; 4 is a micropump; 5 is an electromagnetic valve; 6 is a multi-channel fluorescence detection chip; 7 is a 1-to-8 splitter; 8 is a photoelectric detection sensor; 9 is a first optical fiber connector; 10 is a second optical fiber connector; 11 is a third optical fiber connector; 12 is a first optical fiber interface; 13 is a second optical fiber interface; 14 is a single-axis slide rail, 15 is a slider; 16 is a slide rail motor; 17 is a detection lens; 18 is a detection window; 19 is a reaction chamber; 20 is an integrated circuit board, 21 is a positioning column; 22 is a positioning hole; 23 is an injection port A; 24 is an injection port B; 25 is a control interface; 26 is a power supply interface; 27 is a telemetry interface; 28 is a communication interface; 29 is an excitation light source; 30 is a reaction chamber; 31 is an external handle. DETAILED DESCRIPTION

[0055] In order to make the objectives, technical solutions and advantages of the present disclosure more clearly understood, the present disclosure is further described in detail below in combination with specific embodiments and with reference to the accompanying drawings.

[0056] However, it should be understood that these descriptions are exemplary only and are not intended to limit the scope of the present disclosure. In the following detailed description, for ease of explanation, many specific details are set forth to provide a comprehensive understanding of the embodiments of the present disclosure. However, it is apparent that one or more embodiments may also be implemented without these specific details. In addition, in the following description, the description of the known technology is omitted to avoid unnecessary confusion of the concepts of the present disclosure.

[0057] The terms used herein are only for describing specific embodiments and are not intended to limit the present disclosure. The term "comprising" used herein indicates the existence of features, steps, operations, but does not exclude the existence or addition of one or more other features. It should be noted that all terms (including technical and scientific terms) used herein have the meanings commonly understood by those skilled in the art, unless otherwise defined. It should be noted that the terms used here should be interpreted as having meanings consistent with the context of this specification, and should not be interpreted in an idealized or overly rigid manner.

[0058] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0059] Example 1

[0060] like Figure 1 As shown, this embodiment provides an on-orbit microorganism detection device, including a detection outer box 1, a chip card box 2 inserted into the detection outer box 1, and an optical fiber connection assembly for connecting the detection outer box 1 and the chip card box 2;

[0061] like Figure 2-Figure 3 As shown, exemplarily, the chip card box 2 includes a card box body, a microcavity optical fiber detection chip 3, a perfusion module and a multi-channel fluorescence detection chip 6; wherein the microcavity optical fiber detection chip 3, the perfusion module and the multi-channel fluorescence detection chip 6 are all arranged inside the card box body;

[0062] Specifically, the perfusion module includes a micropump 4 and an electromagnetic valve 5, the liquid outlet end of the electromagnetic valve 5 is connected to the liquid inlet end of the microcavity fiber optic detection chip 3, and the liquid outlet end of the microcavity fiber optic detection chip 3 is connected to the liquid inlet end of the micropump 4, so as to transport culture medium and microorganisms to the microcavity fiber optic detection chip 3.

[0063] Exemplarily, the detection outer box 1 includes an outer box body, a detection module, a control module, a power module and an observation module; wherein the detection module, the control module, the power module and the observation module are all arranged inside the outer box body;

[0064] Specifically, the detection module is connected to the microcavity fiber detection chip 3 by optical fiber to detect the microorganisms in the microcavity fiber detection chip 3; the power module is electrically connected to the control module to provide power supply; the control module is electrically connected to the detection module to receive the microorganism detection result; the control module is electrically connected to the perfusion module to control the perfusion module;

[0065] like Figure 4 As shown, in some embodiments, the detection module includes an excitation light source 29, a 1-to-8 splitter 7, and 8 photoelectric detection sensors 8;

[0066] Specifically, the output end of the excitation light source 29 is connected to the input end of the 1-to-8 splitter 7 by optical fiber, and the output end of the 1-to-8 splitter 7 is connected to the input end of the microcavity fiber optic detection chip 3 by optical fiber; the output end of the microcavity fiber optic detection chip 3 is connected to the input end of the photoelectric detection sensor 8 by optical fiber, and the output end of the photoelectric detection sensor 8 is electrically connected to the input end of the control module respectively.

[0067] In this embodiment, the excitation light source 29 enters the 1-to-8 optical path splitter 7 to divide the excitation light beam into 8 paths and pass through the optical fiber connection component as the input light source to detect and identify the microorganisms in the chip card box 2, and then the collected light signals are transmitted to the 8 photoelectric detection sensors 8 through the optical fiber connection component respectively. After the light signals are converted into digital signals, the microorganisms are detected and analyzed, and then the analysis results are transmitted to the control module, and the laser analysis results of the microorganisms are output to the outside world via the control module.

[0068] like Figure 5 - As shown in 6, illustratively, the optical fiber connection assembly includes a first optical fiber connector 9, a second optical fiber connector 10, a third optical fiber connector 11, a first optical fiber interface 12 and a second optical fiber interface 13;

[0069] Specifically, the first end of the first optical fiber connector 9 is connected to the output end of the splitter 7, and the second end of the first optical fiber connector 9 is connected to the input end of the photoelectric detection sensor 8; the first end of the second optical fiber connector 10 is connected to the third end of the first optical fiber connector 9 through the first optical fiber interface 12, and the second end of the second optical fiber connector 10 is connected to the first end of the third optical fiber connector 11 through the second optical fiber interface 13; the second end of the third optical fiber connector 11 is connected to the input end of the microcavity optical fiber detection chip 3, and the output end of the microcavity optical fiber detection chip 3 is connected to the third end of the third optical fiber connector 11;

[0070] In this embodiment, the first optical fiber interface 12 is arranged on the top surface of the outer box body, and the third optical fiber interface is arranged on the top surface of the card box body. Figure 4 shown.

[0071] like Figure 7 As shown, in some embodiments, the microcavity optical fiber detection chip 3 includes a chip housing, and the chip housing is provided with a liquid inlet, a liquid outlet, a liquid inlet channel, a liquid outlet channel and 8 microcavities;

[0072] Specifically, each microcavity is provided with a sample flow channel for transmitting microorganisms and culture medium and a laser channel for transmitting optical signals; wherein the laser channel can enable the laser to fully cover the detection of microorganisms and culture medium in the sample flow channel;

[0073] Specifically, the liquid inlet end of the liquid inlet channel is connected to the liquid outlet end of the perfusion module through the liquid inlet port; the liquid outlet ends of the liquid inlet channel are respectively connected to the liquid inlet end of the sample channel; the liquid outlet ends of the sample channel are respectively connected to the liquid inlet end of the liquid outlet channel, and the liquid outlet end of the liquid outlet channel is connected to the liquid inlet end of the liquid outlet port;

[0074] The input ends of the laser channels are respectively connected to the second ends of the third optical fiber connectors 11, and the output ends of the laser channels are respectively connected to the third ends of the third optical fiber connectors 11, so as to detect the microorganisms in the eight microcavities in parallel by laser.

[0075] In this embodiment, the microcavity fiber optic detection chip 3 adopts an independent layered design of the optical path and the liquid path, which avoids mutual interference between the optical path and the sample liquid path, prevents mutual cutting of the path channels, improves the stability and reliability of the microcavity fiber optic detection chip 3, and improves the accuracy of microbial detection.

[0076] In some embodiments, the multi-channel fluorescence detection chip 6 includes a sample chamber, a lysis chamber, a liquid separation valve, a negative pressure pump and a plurality of reaction chambers 3019;

[0077] Specifically, the liquid outlet of the sample chamber is connected to the liquid inlet of the negative pressure pump, and the liquid outlet of the negative pressure pump is connected to the liquid inlet of the lysis chamber; the liquid outlet of the lysis chamber is connected to the liquid inlet of the liquid separation valve, and the liquid outlet of the liquid separation valve is respectively connected to the liquid inlet of the plurality of reaction chambers 3019;

[0078] Among them, several reaction chambers 3019 are pre-buried with reaction reagents for microbial fluorescence detection; the sample solution in the sample cavity is pre-set in the sample cavity.

[0079] like Figure 8 As shown, in some embodiments, the observation module includes a single-axis slide rail 14, a slider 15, a slide rail motor 16, a detection lens 17 and a signal processing circuit board;

[0080] The single-axis slide rail 14 is fixedly mounted on the inner side wall of the outer box body, the slider 15 is slidably mounted on the slide rail, and the detection lens 17 is fixedly mounted at the bottom of the slider 15; the slide rail motor 16 is fixedly mounted at the end of the single-axis slide rail 14 to drive the slider 15 to drive the detection lens 17 to reciprocate along the slide rail; the input end of the signal processing circuit board is electrically connected to the output end of the detection lens 17, and the output end of the signal processing circuit board is electrically connected to the input end of the control module;

[0081] like Figure 9-10 As shown, in this embodiment, a detection window 18 is opened at the position of the inner wall of the card box body corresponding to the microcavity reaction chamber 3019 for performing fluorescence detection on the microcavity reaction chamber 3019 through a detection lens 17.

[0082] In this embodiment, different bacterial liquids flow into the plurality of reaction chambers 3019 in the multi-channel fluorescence detection chip 6, and the reaction reagents contain fluorescent components. When different bacterial liquids react with the reagents, fluorescence of different wavelengths will be generated, and the corresponding fluorescence values ​​will also be different. The detection lens 17 collects these fluorescence value signals through the detection window 18 and transmits them to the signal processing circuit board. The signal processing circuit board converts the received fluorescence value analog signal into a digital signal, performs fluorescence analysis, and then sends the fluorescence analysis result of the bacterial liquid to the control module, and outputs the fluorescence analysis result of the bacterial liquid to the outside through the control module.

[0083] In some embodiments, the control module includes an integrated circuit board 20, the input end of the integrated circuit board 20 is electrically connected to the power module, the signal processing circuit board and the output end of the eight photoelectric detection sensors 8 respectively;

[0084] In this embodiment, a control interface 25 is provided at the bottom of the card box body, and the control interface 25 is plugged into the integrated circuit board 20. The output end of the integrated circuit board 20 is electrically connected to the input ends of the micro pump 4, the solenoid valve 5, the liquid separation valve and the negative pressure pump through the control interface 25 to control the operation of the above-mentioned components.

[0085] like Fig.11 As shown, in this embodiment, the integrated circuit board 20 is arranged at the bottom of the outer box body, and at least two positioning holes 22 are opened on the integrated circuit board 20; at least two positioning posts 21 corresponding to the positions of the positioning holes 22 are provided on the card box body, and the positioning posts 21 are inserted into the corresponding positioning holes 22 to fix the chip card box 2.

[0086] In this embodiment, the chip card box 2 and the detection outer box 1 are fixed by plugging, and the chip card box 2 is positioned and inserted into the detection outer box 1 for experiment through the positioning column 21 and the positioning hole 22, ensuring that the astronaut can operate it in one go.

[0087] In some embodiments, the chip card box 2 is provided with an injection port A23, an injection port B24 and at least one outlet; wherein the liquid outlet ends of the injection port A23 and the injection port B24 are connected to the liquid inlet end of the solenoid valve 5; and the liquid inlet end of at least one outlet is connected to the liquid outlet end of the micro pump 4.

[0088] In some embodiments, a waste liquid bag is further included, wherein the liquid inlet end of the waste liquid bag is connected to the liquid outlet end of at least one sample outlet to collect waste liquid after microorganism detection in the microcavity optical fiber detection chip 3 .

[0089] In some embodiments, an external handle 31 is installed on the chip card box 2 to lift the chip card box 2 to facilitate the chip card box 2 to enter or exit the detection outer box 1.

[0090] In some embodiments, the detection outer box 1 also includes a power supply interface 26, a telemetry interface 27 and a communication interface 28; wherein the power supply interface 26, the telemetry interface 27 and the communication interface 28 are all fixed on the detection outer box 1 in the form of standard electrical connectors.

[0091] The on-orbit microbial detection device in this embodiment is suitable for aerospace basic experimental cabinets. The aerospace basic experimental cabinet is an important component of the space station and provides a key experimental platform for space science and application research. The aerospace basic experimental cabinet can provide a variety of experimental spaces, adapt to the installation of multiple types and specifications of load units in different forms, and realize the free matching of load units on orbit to maximize the test needs; when the on-orbit microbial detection device in this embodiment is in use, it is necessary to place the detection device in the test space and connect it to the information management subsystem of the aerospace basic experimental cabinet through the power supply interface 26, the telemetry interface 27 and the communication interface 28; this embodiment supports two control modes: remote sensing experiments and on-site operation experiments; among them, the control between the information management subsystem of the aerospace basic experimental cabinet and each module in the microbial detection device adopts real-time mode control, and the on-site operation experiment requires limited participation of astronauts; remote sensing experiments need to resolve possible channel conflicts, so data injection is adopted, that is, after the specific operation experiment process is designed by the ground, the overall data is injected into the instrument, and then the fully automatic experiment is started by remote sensing commands.

[0092] In summary, in this embodiment, a microcavity optical fiber detection chip 3 is designed based on the microbial detection technology of Whispering Gallery Mode (WGM), and a multi-channel fluorescence detection chip 6 is designed based on the microbial detection technology of CRISPR / Cas12b-LAMP principle. The two detection chips are arranged in the same chip card box 2 without interacting with each other and independently completing the detection. Microbial laser detection and microbial fluorescence detection can be realized simultaneously, which greatly improves the detection efficiency and accuracy, thereby reducing the time and resources required for the experiment.

[0093] The detection outer box in this embodiment can not only complete the injection of samples into the chip card box 2 and the collection of detection data, but also complete command communication and data communication with the outside, thereby realizing efficient detection of on-orbit microorganisms, significantly improving detection efficiency, reducing dependence on ground support, and providing strong technical support for on-orbit microbial detection.

[0094] In this embodiment, the microcavity fiber optic detection chip 3 adopts an independent layered design of the laser light path and the sample liquid path, which avoids mutual interference between the laser light path and the sample liquid path, prevents mutual cutting of the path channels, improves the stability and reliability of the microcavity fiber optic detection chip 3, and improves the accuracy of microbial detection.

[0095] The above description is only a preferred embodiment of the present invention. It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention.

Claims

1. An on-orbit microbial detection device, characterized in that: It includes a detection outer box, a chip card box inserted into the detection outer box, and an optical fiber connection component for connecting the detection outer box and the chip card box with an optical fiber; Chip card box includes: Card box body; Microcavity fiber optic detection chip, used to store microorganisms for detection; A perfusion module is connected to the microcavity optical fiber detection chip and is used to deliver culture medium and microorganisms to the microcavity optical fiber detection chip; The inspection box includes: Outer box body; A detection module is connected to the microcavity optical fiber detection chip optical fiber to detect microorganisms in the microcavity optical fiber detection chip; The control module is electrically connected to the detection module to receive the microorganism detection result; the control module is also electrically connected to the perfusion module to control the perfusion module; A power module, electrically connected to the control module, for providing power supply; The microcavity optical fiber detection chip and the perfusion module are both arranged inside the card box body; the detection module, the control module and the power supply module are all arranged inside the outer box body.

2. The device according to claim 1, characterized in that The detection module includes an excitation light source, a splitter and a plurality of photoelectric detection sensors; The output end of the excitation light source is connected to the input end optical fiber of the splitter, and the output end of the splitter is connected to the input end optical fiber of the microcavity optical fiber detection chip; The output end of the microcavity optical fiber detection chip is respectively connected to the optical fibers of the input ends of a plurality of photoelectric detection sensors, and the output ends of the plurality of photoelectric detection sensors are electrically connected to the input end of the control module.

3. The device according to claim 1, characterized in that The microcavity optical fiber detection chip comprises a chip shell, and the chip shell is provided with a liquid inlet, a liquid outlet, a liquid inlet flow channel, a liquid outlet flow channel and a plurality of microcavities; The liquid inlet end of the liquid inlet channel is connected to the liquid outlet end of the perfusion module through the liquid inlet port; the liquid outlet ends of the liquid inlet channel are respectively connected to the liquid inlet ends of the plurality of microcavities; the liquid outlet ends of the plurality of microcavities are respectively connected to the liquid inlet ends of the liquid outlet channel, and the liquid outlet end of the liquid outlet channel is connected to the liquid inlet end of the liquid outlet port; The plurality of microcavities are communicated with the optical fiber connection components respectively.

4. The device according to claim 1, characterized in that The chip card box also includes a multi-channel fluorescence detection chip; The multi-channel fluorescence detection chip includes a sample chamber, a lysis chamber, a liquid separation valve, a negative pressure pump and a plurality of reaction chambers; The liquid outlet of the sample chamber is connected to the liquid inlet of the negative pressure pump, and the liquid outlet of the negative pressure pump is connected to the liquid inlet of the lysis chamber; the liquid outlet of the lysis chamber is connected to the liquid inlet of the liquid separation valve, and the liquid outlet of the liquid separation valve is respectively connected to the liquid inlet of a plurality of reaction chambers; The reaction chambers are all pre-buried with reaction reagents for microbial fluorescence detection.

5. The device according to claim 1, characterized in that The perfusion module includes a micro pump and a solenoid valve; the liquid outlet of the solenoid valve is connected to the liquid inlet of the microcavity optical fiber detection chip, and the liquid outlet of the microcavity optical fiber detection chip is connected to the liquid inlet of the micro pump; The card box is provided with at least two sample inlets and at least one sample outlet; the liquid outlet ends of the at least two sample inlets are connected to the liquid inlet end of the electromagnetic valve; the liquid inlet end of the at least one sample outlet is connected to the liquid outlet end of the micro pump.

6. The device according to claim 2, characterized in that The optical fiber connection assembly includes a first optical fiber connector, a second optical fiber connector, a third optical fiber connector, a first optical fiber interface, and a second optical fiber interface; The first end of the first optical fiber connector is connected to the output end of the splitter, and the second end of the first optical fiber connector is connected to the input end of the photoelectric detection sensor; The first end of the second optical fiber connector is connected to the third end of the first optical fiber connector through the first optical fiber interface, and the second end of the second optical fiber connector is connected to the first end of the third optical fiber connector through the second optical fiber interface; the second end and the third end of the third optical fiber connector are both connected to the microcavity optical fiber detection chip; The first optical fiber interface is arranged on the top surface of the outer box body, and the third optical fiber interface is arranged on the top surface of the card box body.

7. The device according to claim 4, characterized in that The detection outer box also includes an observation module disposed inside the outer box body; the observation module includes a single-axis slide rail, a slider, a slide rail motor, a detection lens and a signal processing circuit board; The single-axis slide rail is fixedly installed on the inner side wall of the outer box body, the slider is slidably arranged on the slide rail, and the detection lens is fixedly arranged at the bottom of the slider; the slide rail motor is fixedly arranged at the end of the single-axis slide rail, and is used to drive the slider to drive the detection lens to move back and forth along the slide rail; the input end of the signal processing circuit board is electrically connected to the output end of the detection lens, and the output end of the signal processing circuit board is electrically connected to the input end of the control module; A detection window is provided on the inner wall of the card box body at a position corresponding to the microcavity reaction chamber, so as to perform fluorescence detection on the microcavity reaction chamber through a detection lens.

8. The device according to claim 4, characterized in that A control interface is provided at the bottom of the cartridge body, the input end of the control interface is electrically connected to the output end of the control module, and the output end of the control interface is electrically connected to the input ends of the perfusion module, the liquid separation valve and the negative pressure pump respectively.

9. The device according to claim 1, 7 or 8, characterized in that The control module comprises an integrated circuit board, the input end of the integrated circuit board is electrically connected to the output end of the power module and the detection module respectively, and the output end of the integrated circuit board is electrically connected to the input end of the perfusion module; The integrated circuit board is arranged at the bottom of the outer box body, and at least two positioning holes are opened on the integrated circuit board; the card box body is provided with at least two positioning posts corresponding to the positions of the positioning holes, and the positioning posts are inserted into the corresponding positioning holes to fix the chip card box.

10. The device according to claim 1, characterized in that The detection outer box also includes a power supply interface, a telemetry interface and a communication interface.

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