Complex enzyme preparation for improving digestive function of intestinal tracts of pets and preparation method of complex enzyme preparation

Abstract

The invention relates to a compound enzyme preparation for improving the digestive function of intestinal tracts of pets and a preparation method of the compound enzyme preparation. The compound enzyme preparation is prepared from the following raw materials in percentage by mass: 36-45% of a compound plant fermentation product, 18-25% of compound enzyme, 14-18% of shea butter, 10-16% of fucoidan polysaccharide sulfate and 11-19% of bacillus subtilis, wherein the compound plant fermentation product is obtained by drying a compound fermentation solution obtained by mixing and fermenting pumpkin vines, corn straws and melastoma dodecandrum with lactobacillus bulgaricus. The compound enzyme preparation promotes and improves the absorption effect of pet intestinal tracts on crude protein / protein, reduces the concentration of ammonia in excrement, reduces odor, can reduce environmental pollution and improve the odor environment of common life of pets and people in the actual use process, and has good practical significance.

Description

Technical Field

[0001] The invention belongs to the technical field of pet intestinal care, and specifically relates to a composite enzyme preparation for improving the intestinal digestive function of a pet and a preparation method thereof. Background Art

[0002] As people's living standards improve, the number of pet dogs continues to rise, and the demand for dog food also increases accordingly. In particular, high-protein dog food with balanced nutrition is favored by dog ​​owners. The advantage of feeding pets with high-protein dog food is that it can promote the growth and development of pets, ensure their healthy growth, enhance their immunity, maintain muscle mass, etc.; however, high-protein dog food may also cause poor gastrointestinal absorption in pets. The ammonia content in pet feces mainly comes from undigested protein in the intestine. These substances will produce ammonia under the action of intestinal bacteria, resulting in the problem of increased odor in pet defecation. In the prior art, there are few studies on compound enzyme preparations that promote or improve the absorption of protein by pet intestines. In order to solve the above technical problems, we provide a compound enzyme preparation that improves the digestive function of pet intestines and a preparation method thereof. Summary of the invention

[0003] The purpose of the present invention is to solve the above problems and provide a composite enzyme preparation for improving the digestive function of the pet's intestines and a preparation method thereof.

[0004] The present invention achieves the above-mentioned purpose through the following technical solutions:

[0005] The invention provides a composite enzyme preparation for improving the digestive function of the intestinal tract of pets, comprising the following raw materials in percentage by mass: 36-45% of composite plant fermentation product, 18-25% of composite enzyme, 14-18% of shea butter, 10-16% of brown algae polysaccharide sulfate, and 11-19% of Bacillus subtilis; wherein the composite plant fermentation product is obtained by drying the composite fermentation liquid obtained by mixed fermentation of pumpkin vines, corn stalks, and ground radish with Lactobacillus bulgaricus.

[0006] As a further optimization scheme of the present invention, the mass ratio of the pumpkin vine, corn stalk and ground radish is 1:3:0.3-1.2.

[0007] As a further optimization scheme of the present invention, the composite enzyme is 1500u / g amylase, 2000u / g cellulase, 2200u / g protease, 2800u / g xylanase and 1100u / g β-glucanase.

[0008] The present invention also provides a method for preparing a composite enzyme preparation for improving the intestinal digestive function of pets, and the specific steps are as follows:

[0009] Step 1: Wash, dry, crush and evenly mix pumpkin vines, corn stalks and ground radish to obtain a mixed fermentation raw material, add Lactobacillus bulgaricus to the mixed fermentation raw material after ultrasonic treatment, mix well and allow it to be fully fermented under anaerobic conditions, and dry the composite fermentation liquid obtained after anaerobic fermentation to obtain a powdered composite plant fermentation product;

[0010] Step 2: Mix the composite enzyme, shea butter, fucoidan sulfate, Bacillus subtilis and the powdered composite plant fermentation product prepared in step 1 to obtain a composite enzyme preparation.

[0011] As a further optimization scheme of the present invention, in step one, the moisture content of the mixed fermentation raw material is 45%-55%, and the length is 8-15 mm.

[0012] As a further optimization scheme of the present invention, in step one, the mixed fermentation raw material is ultrasonically treated at a power of 150 W for 12-18 min.

[0013] As a further optimization scheme of the present invention, in step 1, 6-8 g of Lactobacillus bulgaricus is added to every 100 g of the mixed fermentation raw material, the fermentation temperature is 42-45° C., and the fermentation time is 5-7 days.

[0014] The beneficial effects of the present invention are:

[0015] 1) The composite enzyme preparation of the present invention promotes and improves the absorption of crude protein / protein by the pet's intestine, thereby reducing the ammonia concentration in the feces and reducing the odor. In actual use, it can reduce environmental pollution and improve the odor environment for pets and humans to live together, which has good practical significance;

[0016] 2) The composite plant fermentation product in the composite enzyme preparation of the present invention is obtained by drying the composite fermentation liquid after mixed fermentation of pumpkin vines, corn stalks and ground radish with Bulgarian Lactobacillus. Experimental verification shows that there may be a synergistic effect between pumpkin vines, corn stalks and ground radish, which can significantly improve the digestibility of crude protein in pets. DETAILED DESCRIPTION

[0017] The present application is described in further detail below. It is necessary to point out that the following specific implementation methods are only used to further illustrate the present application and cannot be understood as limiting the scope of protection of the present application. Technicians in this field can make some non-essential improvements and adjustments to the present application based on the above application content.

[0018] 1. Materials

[0019] Unless otherwise specified, the methods used in this example are conventional methods known to those skilled in the art, and the materials used for preparation, such as raw materials and reagents, are all commercially available products unless otherwise specified.

[0020] 2. Methods

[0021] In order to study the effects of different proportions of the raw materials for preparing the composite enzyme preparation and different components and proportions of the composite plant fermentation product on improving the intestinal digestive function of pets, the following Examples 1-5, Comparative Examples 1-6 and a blank group were set, as shown below:

[0022] Example 1

[0023] A composite enzyme preparation for improving the digestive function of the pet's intestines comprises the following raw materials in percentage by mass: 36-45% of composite plant fermentation product, 18-25% of composite enzyme, 14-18% of shea butter, 10-16% of brown algae polysaccharide sulfate, and 11-19% of Bacillus subtilis; wherein the composite plant fermentation product is obtained by drying the composite fermentation liquid obtained by mixed fermentation of pumpkin vines, corn stalks, and radish with Lactobacillus bulgaricus.

[0024] It should be noted that in the following Examples 1-5, Comparative Examples 1-6 and the blank group, the number of viable bacteria of the Bacillus subtilis was 5.3×10 8 CFU / g.

[0025] Preferably, the complex enzyme is amylase 1500u / g, cellulase 2000u / g, protease 2200u / g, xylanase 2800u / g, and β-glucanase 1100u / g.

[0026] A method for preparing a composite enzyme preparation for improving the intestinal digestive function of pets, the specific steps are as follows:

[0027] Step 1: Wash, dry, crush and evenly mix pumpkin vines, corn stalks and ground radish to obtain a mixed fermentation raw material, add Lactobacillus bulgaricus to the mixed fermentation raw material after ultrasonic treatment, mix well and allow it to be fully fermented under anaerobic conditions, and dry the composite fermentation liquid obtained after anaerobic fermentation to obtain a powdered composite plant fermentation product;

[0028] The moisture content of the mixed fermentation raw material is 45%-55%, and the length is 8-15 mm. It should be noted that in the following Examples 1-5, Comparative Examples 1-6 and the blank group, the moisture content of the mixed fermentation raw material is 48%, and the length is 12 mm;

[0029] The mixed fermentation raw material ultrasonic treatment is 150W power ultrasonic treatment for 12-18min. It should be noted that in the following Examples 1-5, Comparative Examples 1-6 and the blank group, the mixed fermentation raw material ultrasonic treatment is 150W power ultrasonic treatment for 15min;

[0030] 6-8 g of Lactobacillus bulgaricus was added to every 100 g of mixed fermentation raw materials (7 g was added in the following Examples 1-5, Comparative Examples 1-6 and the blank group), the fermentation temperature was 42-45 ° C, and the fermentation time was 5-7 days. It should be noted that in the following Examples 1-5, Comparative Examples 1-6 and the blank group, the fermentation temperature was 43 ° C, the fermentation time was 6 days, and the number of viable bacteria of Lactobacillus bulgaricus was 8.7 × 10 7 CFU / g;

[0031] Step 2: Mix the composite enzyme, shea butter, fucoidan sulfate, Bacillus subtilis and the powdered composite plant fermentation product prepared in step 1 to obtain a composite enzyme preparation.

[0032] The difference between Examples 1-5 is that the proportions of the raw materials for preparing the composite enzyme preparation are different and the proportions of the mixed fermentation raw materials for the composite plant fermentation are different, as shown in Table 1:

[0033] Table 1 The proportion of each component of the raw materials for the preparation of the complex enzyme preparation and the proportion of the mixed fermentation raw materials

[0034]

[0035]

[0036] Comparative Example 1

[0037] In this comparative example, a composite enzyme preparation for improving the intestinal digestive function of pets includes the following raw materials in percentage by mass: 40% composite plant fermentation product, 22% composite enzyme, 14% shea butter, 10% brown algae polysaccharide sulfate, and 14% Bacillus subtilis; wherein the composite plant fermentation product is obtained by drying the composite fermentation liquid after mixed fermentation of corn stalks and ground radish, and the only difference from Example 4 is that the pumpkin vine is replaced by corn stalks of equal mass, that is, the mass ratio of corn stalks to ground radish is 4:0.8, and the rest is consistent with Example 4.

[0038] Comparative Example 2

[0039] In this comparative example, a composite enzyme preparation for improving the intestinal digestive function of pets includes the following raw materials in percentage by mass: 40% composite plant fermentation product, 22% composite enzyme, 14% shea butter, 10% brown algae polysaccharide sulfate, and 14% Bacillus subtilis; wherein the composite plant fermentation product is obtained by drying the composite fermentation liquid after mixed fermentation of pumpkin vines and ground radish, and the only difference from Example 4 is that the corn stalks are replaced by pumpkin vines of equal mass, that is, the mass ratio of pumpkin vines to ground radish is 4:0.8, and the rest are consistent with Example 4.

[0040] Comparative Example 3

[0041] In this comparative example, a composite enzyme preparation for improving the intestinal digestive function of pets includes the following raw materials in percentage by mass: 40% composite plant fermentation product, 22% composite enzyme, 14% shea butter, 10% brown algae polysaccharide sulfate, and 14% Bacillus subtilis; wherein the composite plant fermentation product is obtained by drying the composite fermentation liquid after mixed fermentation of pumpkin vines and corn stalks, and the only difference from Example 4 is that the corn stalks of equal mass are used to replace the ground radish, that is, the mass ratio of pumpkin vines to corn stalks is 1:3.8, and the rest are consistent with Example 4.

[0042] Comparative Example 4

[0043] In this comparative example, a composite enzyme preparation for improving the intestinal digestive function of pets includes the following raw materials in percentage by mass: 40% composite plant fermentation product, 22% composite enzyme, 14% shea butter, 10% brown algae polysaccharide sulfate, and 14% Bacillus subtilis; wherein the composite plant fermentation product is obtained by drying the composite fermentation liquid after fermentation of pumpkin vines, and the only difference from Example 4 is that pumpkin vines of equal mass are used instead of corn stalks and ground radish, and the rest are consistent with Example 4.

[0044] Comparative Example 5

[0045] In this comparative example, a composite enzyme preparation for improving the intestinal digestive function of pets includes the following raw materials in percentage by mass: 40% composite plant fermentation product, 22% composite enzyme, 14% shea butter, 10% brown algae polysaccharide sulfate, and 14% Bacillus subtilis; wherein the composite plant fermentation product is obtained by drying the composite fermentation liquid after mixed fermentation of corn stalks, and the only difference from Example 4 is that pumpkin vines and ground radish are replaced by corn stalks of equal mass, and the rest are consistent with Example 4.

[0046] Comparative Example 6

[0047] In this comparative example, a composite enzyme preparation for improving the intestinal digestive function of pets includes the following raw materials in percentage by mass: 40% composite plant fermentation product, 22% composite enzyme, 14% shea butter, 10% brown algae polysaccharide sulfate, and 14% Bacillus subtilis; wherein the composite plant fermentation product is obtained by drying the composite fermentation liquid after mixed fermentation of Euphorbia obesa, and the only difference from Example 4 is that pumpkin vines and corn stalks are replaced by Euphorbia obesa of equal mass, and the rest are consistent with Example 4.

[0048] Blank Group

[0049] In this comparative example, a composite enzyme preparation for improving the intestinal digestive function of pets includes the following raw materials in percentage by mass: 22% composite enzyme, 14% shea butter, 10% brown algae polysaccharide sulfate, and 54% Bacillus subtilis; the only difference from Example 4 is that the composite plant fermentation product is replaced by an equal mass of Bacillus subtilis, and the rest is consistent with Example 4.

[0050] 3. Experiment

[0051] 90-day-old pet dogs weighing between 1.7-2.3 kg, half male and half female, were divided into 12 groups, with two dogs in each group (one male and one female), and fed with a basic diet according to a conventional feeding and management process, and fed with 2 g of the composite enzyme preparation for improving the intestinal digestive function of pets prepared in Examples 1-5 and Comparative Examples 1-6 of the present invention and a blank group every day, and pet feces odor test and nutrient apparent digestibility test were performed in the first three days of feeding (recorded as Group A) and the last three days within 30 days of feeding (recorded as Group B, the total feeding time is 30 days, and the last three days within 30 days of feeding are the 28th, 29th, and 30th days of feeding), respectively, and the average values ​​were taken and recorded in Tables 3-4. In addition, the formula of the pet basic diet was prepared with reference to GB / T31216-2014 Complete Pet Food Dog Food, as shown in Table 2:

[0052] Table 2 Basic diet composition and nutritional level

[0053]

[0054]

[0055] The nutrient apparent digestibility test is to determine the nutrient apparent digestibility according to the endogenous indicator method, wherein, nutrient apparent digestibility (%) = [(nutrient content of ingestion - nutrient content of excrement) / nutrient content of ingestion] × 100%, and the data are shown in Table 3:

[0056] Table 3 Statistics of apparent digestibility of nutrients

[0057]

[0058]

[0059] Note: The above data have been verified by variance.

[0060] The pet feces odor test is to detect the feces ammonia concentration of group A and group B respectively according to the chloroform method, and calculate the reduction rate of ammonia concentration in group B compared with group A, wherein the calculation formula of ammonia concentration reduction rate is, ammonia concentration reduction rate = [(ammonia concentration in group A feces - ammonia concentration in group B feces) / ammonia concentration in group A feces] × 100%, as shown in Table 4:

[0061] Table 4 Statistics of ammonia concentration reduction rate

[0062]

[0063]

[0064] Note: The above data have been verified by variance.

[0065] Experimental conclusion: From Tables 3-4, it can be seen that the effect of the composite enzyme preparation prepared in Example 4 on improving the digestive function of the pet's intestines is significantly better than that of Examples 1-3 and Example 5, and compared with the blank group, the composite enzyme preparation in Example 4 can better promote the absorption of crude protein by the pet's intestines, and can significantly reduce the ammonia concentration in the pet's feces and reduce the odor, indicating that the pet's intestines are significantly repaired and improved;

[0066] By comparing Example 4 in Table 3 with Comparative Examples 1-6, it can be seen that the composite enzyme preparation obtained by fermenting pumpkin vines / corn stalks / earth radish alone or fermenting pumpkin vines, corn stalks, and ear radish in combination has little effect on improving the apparent digestibility of crude protein in pets, while the composite enzyme preparation obtained by mixed fermentation of pumpkin vines, corn stalks, and ear radish has a significant effect on improving the apparent digestibility of crude protein in pets. It is speculated that there may be a synergistic effect between pumpkin vines, corn stalks, and ear radish, which can significantly improve the digestibility of crude protein in pets;

[0067] By comparing Example 4 in Table 4 with Comparative Examples 1-6, it can be learned that the composite enzyme preparation obtained by fermenting pumpkin vines / corn stalks / earth radish alone or fermenting pumpkin vines, corn stalks and ear radish in combination, the reduction rate of ammonia concentration in pet feces is slightly improved, between 11.6% and 18.9%, while the composite enzyme preparation obtained by mixed fermentation of pumpkin vines, corn stalks and ear radish, the reduction rate of ammonia concentration in pet feces is significantly improved, which is 44.7%. This shows that there may be a synergistic effect between pumpkin vines, corn stalks and ear radish, which improves the absorption of protein by the pet's intestine, reduces the ammonia concentration in feces, and reduces odor. In actual use, it can reduce environmental pollution and improve the odor environment for pets and humans to live together, which has good practical significance.

[0068] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention.

Claims

1. A composite enzyme preparation for improving the digestive function of the pet's intestines, characterized in that: The invention comprises the following raw materials in percentage by weight: 36-45% of composite plant fermentation product, 18-25% of composite enzyme, 14-18% of shea butter, 10-16% of brown algae polysaccharide sulfate and 11-19% of Bacillus subtilis; wherein the composite plant fermentation product is obtained by drying the composite fermentation liquid obtained by mixed fermentation of pumpkin vines, corn stalks and ground radish with Lactobacillus bulgaricus.

2. The composite enzyme preparation for improving the digestive function of the pet's intestine according to claim 1, characterized in that: The mass ratio of the pumpkin vine, corn stalk and ground radish is 1:3:0.3-1.

2.

3. The composite enzyme preparation for improving the digestive function of the pet's intestine according to claim 1, characterized in that: The complex enzyme is amylase 1500u / g, cellulase 2000u / g, protease 2200u / g, xylanase 2800u / g, and beta-glucanase 1100u / g.

4. A method for preparing a composite enzyme preparation for improving the digestive function of the pet's intestines as claimed in any one of claims 1 to 3, characterized in that: The specific steps are as follows: Step 1: Wash, dry, crush and evenly mix pumpkin vines, corn stalks and ground radish to obtain a mixed fermentation raw material, add Lactobacillus bulgaricus to the mixed fermentation raw material after ultrasonic treatment, mix well and allow it to be fully fermented under anaerobic conditions, and dry the composite fermentation liquid obtained after anaerobic fermentation to obtain a powdered composite plant fermentation product; Step 2: Mix the composite enzyme, shea butter, fucoidan sulfate, Bacillus subtilis and the powdered composite plant fermentation product prepared in step 1 to obtain a composite enzyme preparation.

5. The method for preparing a composite enzyme preparation for improving the intestinal digestive function of pets according to claim 4, characterized in that: In step 1, the moisture content of the mixed fermentation raw material is 45%-55%, and the length is 8-15 mm.

6. The method for preparing a composite enzyme preparation for improving the intestinal digestive function of pets according to claim 4, characterized in that: In step 1, the mixed fermentation raw material is ultrasonically treated at a power of 150 W for 12-18 min.

7. The method for preparing a composite enzyme preparation for improving the intestinal digestive function of pets according to claim 4, characterized in that: In step 1, 6-8 g of Lactobacillus bulgaricus is added to every 100 g of the mixed fermentation raw material, the fermentation temperature is 42-45° C., and the fermentation time is 5-7 days.

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