Preparation method for producing biological bacterial fertilizer from lincomycin waste mushroom dregs

Abstract

The invention discloses a preparation method for producing a biological bacterial fertilizer from lincomycin waste mushroom dregs, and relates to the technical field of fertilizer preparation, and the preparation method comprises the following steps: pretreatment of lincomycin waste mushroom dregs, microbial inoculation, strain fermentation, trace element addition and liquid fertilizer finished product detection. The method has the advantages that the waste lincomycin residues are alternately soaked with diluted hydrochloric acid and a sodium hydroxide solution, various impurities on the surfaces and in the waste lincomycin residues can be fully dissolved, alkaline harmful substances and pollutants combined with metal ions can be decomposed under the acidic condition, and the waste lincomycin residues can be recycled under the alkaline condition. The micro-bubbles can decompose acidic residual antibiotics and organic substances, in the continuous aeration process, on one hand, the micro-bubbles can scour the surfaces of mushroom dregs to enable impurities to fall off and be dissolved more easily, on the other hand, the content of dissolved oxygen in liquid is increased, favorable conditions are created for follow-up microorganism survival, and meanwhile oxidative decomposition of harmful substances is facilitated.

Description

Technical Field

[0001] The invention relates to the technical field of fertilizer preparation, in particular to a method for preparing biological bacterial fertilizer by using lincomycin waste bacterial residue. Background Art

[0002] With the wide application of lincomycin in the pharmaceutical field, its production scale is constantly expanding, and the output of lincomycin waste residue is also increasing. In the current industrial production situation, the treatment of lincomycin waste residue has become a key issue that needs to be solved urgently. On the one hand, most of the traditional methods for treating lincomycin waste residue have obvious defects. The common landfill treatment method not only requires a large amount of land resources, but also the harmful substances in the waste residue may penetrate into the soil and groundwater over time, causing serious soil and water pollution, posing a huge threat to the balance of the ecological environment. Although incineration can reduce the volume of waste residue to a certain extent, a large amount of harmful gases, such as sulfur dioxide and nitrogen oxides, will be generated during the incineration process. These gases will cause air pollution when discharged into the atmosphere. At the same time, highly toxic substances such as dioxins may also be generated, which are extremely harmful to human health and the ecological environment. On the other hand, from the perspective of resource utilization, lincomycin waste residue actually contains rich organic matter, protein and other nutrients. If it can be reasonably utilized, it will have extremely high value. However, some existing methods for converting it into useful products are not perfect. In the field of biological fertilizer preparation, the existing methods of using lincomycin waste residue to produce biological fertilizer have many shortcomings. For example, in the pretreatment stage, it is difficult to effectively remove the residual lincomycin antibiotics and other harmful impurities in the waste residue, which not only affects the activity and growth of the bacteria in the subsequent microbial fermentation process, but also the prepared fertilizer may contain antibiotic residues and cause potential harm to the soil ecosystem. In the microbial fermentation stage, the selection and matching of bacteria are not reasonable, resulting in low conversion efficiency of nutrients and unsatisfactory fertilizer efficiency. At the same time, in terms of the stability of the fertilizer, the products prepared by the existing methods are prone to stratification, precipitation and other phenomena, which affect its use effect and storage period. In addition, there is a lack of effective treatment and reuse means for the waste liquid generated in the production process, resulting in waste of resources and secondary pollution of the environment. To this end, we propose a preparation method for producing biological fertilizer from lincomycin waste residue. Summary of the invention

[0003] The purpose of the present invention is to provide a method for preparing biological fertilizer by using lincomycin waste residue.

[0004] In order to solve the problems raised in the above background technology, the present invention provides the following technical solutions: a preparation method for producing biological fertilizer from lincomycin waste residue, comprising pretreatment of lincomycin waste residue, microbial inoculation, bacterial fermentation, trace element addition and liquid fertilizer finished product detection, and the specific operation steps of producing biological fertilizer from lincomycin waste residue are as follows: Step 1: Place the lincomycin waste residue in a cleaning tank and add acid and alkali solutions to alternately soak it for pretreatment, and after the treatment, rinse it with clean water until it is neutral, and continue aeration during the treatment process to obtain the cleaned residue; Step 2: Add the cleaned mushroom residue into a high-pressure cooking pot, add cooking liquid and cook at high temperature, add a composite enzyme preparation for enzymolysis after cooling, and add activated carbon for adsorption to obtain purified mushroom residue; Step 3, selecting Bacillus subtilis, Bacillus gelatinus and Rhodopseudomonas palustris as composite microbial agents and activating and culturing them, mixing them after the cultivation and inoculating them into the purified bacterial residue, adding nutrient solution for inoculation and cultivation, and obtaining liquid bacterial fertilizer after the cultivation; Step 4: adding nutrients and trace element solution to the liquid fertilizer, stirring and mixing, adding a polymer stabilizer, and ultrasonically dispersing the mixture to obtain a stable liquid biological fertilizer product; Step 5: Collect and treat the waste liquid generated in step 1 to step 4, and repeat the above steps for secondary fermentation. Perform quality inspection on the obtained liquid bio-fertilizer product, and fill and bag it after passing the inspection.

[0005] As a further solution of the present invention: in the step 1, the lincomycin waste residue is placed in a special corrosion-resistant cleaning tank, and is soaked in a dilute hydrochloric acid solution with a pH value of 3-4 for 30 min-60 min. During the process, stirring is continued at a stirring rate of 30 r / min-50 r / min. After the soaking is completed, the acid is discharged, and it is rinsed to neutrality with clean water, and then soaked in a sodium hydroxide solution with a pH value of 9-10 for 50 min-80 min. During the process, stirring is continued at a stirring rate of 30 r / min-50 r / min. After the soaking is completed, it is rinsed again with clean water to neutrality. During the acid-base alternating soaking, an aeration device is used to continuously introduce tiny bubbles into the cleaning solution, and the ventilation volume is 0.8 m3 per cubic meter of cleaning liquid. 3 -1.2m 3 The cleaning time is controlled at 45min-60min, and after completion, the cleaned fungus residue is obtained.

[0006] As a further scheme of the present invention: in the step 2, the cleaned mushroom residue is transferred to a high-pressure cooking pot, and a cooking liquid prepared by mixing deionized water and ethanol solution in a ratio of 3:1 is added, the mass ratio of mushroom residue to cooking liquid is 1:2-3, and the mixture is cooked at 110°C-130°C and a pressure of 0.15MPa-0.25MPa for 60min-90min. Intermittent stirring is adopted during the cooking process, and the mixture is stirred for 10min and then stopped for 5min. The stirring speed is 60r / min-80r / min. After the cooking is completed, the standby mushroom residue is obtained.

[0007] As a further scheme of the present invention: in the step 2, the spare mushroom residue after cooking is cooled to 40°C-50°C, and then a composite enzyme preparation is added for enzymatic hydrolysis, wherein the composite enzyme preparation is prepared by mixing lipase, mannanase and chitin in a ratio of 3:2:1, and the addition amount of the composite enzyme preparation is 40-60 enzyme activity units per kilogram of the spare mushroom residue. At the same time, activated carbon powder accounting for 2%-3% of the mass of the spare mushroom residue is added, and enzymatic hydrolysis is carried out for 3h-4h at a pH value of 6-7. After adsorption is completed, purified mushroom residue is obtained.

[0008] As a further solution of the present invention: In the step 3, Bacillus subtilis, Bacillus jelly-like and Rhodopseudomonas palustris are selected as composite microbial agents, and these three bacteria are activated and cultured in their respective activation culture media. The specific components of the three groups of activation culture media are as follows: The activation medium for Bacillus subtilis contains 10g / L-12g / L of peptone, 5g / L-8g / L of beef extract and 3g / L-5g / L of sodium chloride, the activation medium for Bacillus gelatinus contains 8g / L-10g / L of sucrose, 2g / L-3g / L of ammonium sulfate and 1g / L-2g / L of dipotassium hydrogen phosphate, and the activation medium for Rhodopseudomonas palustris contains 3g / L-5g / L of sodium acetate, 1g / L-2g / L of sodium bicarbonate and 0.5g / L-1g / L of magnesium sulfate. The three bacteria are inoculated in corresponding culture media respectively and cultured at 30℃-32℃ for 24h-36h respectively. The three activated bacteria are mixed in a volume ratio of 2:2:1 to prepare a composite microbial culture solution, and the composite microbial culture solution is inoculated into the purified bacterial residue in a mass ratio of 1:5-8 to the pretreated bacterial residue.

[0009] As a further scheme of the present invention: in the step three, a mixed nutrient solution containing 3g / L-5g / L of glucose, 1g / L-2g / L of yeast powder and 2g / L-3g / L of corn steep liquor is added to the purified residue inoculated with the composite microbial liquid, and cultured for 4d-5d at a temperature of 33°C-35°C and a light intensity of 2000lx-3000lx. During the culture process, the mixture is slowly stirred every 8h at a stirring speed of 30r / min-40r / min, and liquid fertilizer is obtained after mixing.

[0010] As a further solution of the present invention: in the step 4, after the liquid bacterial fertilizer is cultured, a fine powder is added thereto which is ground into a mixture of fish bone powder, soybean meal powder and seaweed powder in a ratio of 2:3:1, wherein the particle size of the fine powder is between 0.08 mm and 0.1 mm, and the amount added is 5% to 8% of the total mass of the liquid bacterial fertilizer. At the same time, a trace element solution is added, wherein the trace element solution contains boron, zinc and molybdenum, and the concentrations are 10 mg to 15 mg of boron, 20 mg to 30 mg of zinc and 5 mg to 8 mg of molybdenum per liter of solution. The amount of chitosan and xanthan gum is 0.5%-1% of the volume of the bacterial liquid. After mixing, a natural polymer stabilizer is added. A mixture of chitosan and xanthan gum is selected. Chitosan and xanthan gum are mixed in a mass ratio of 3:2. The amount of addition is 1%-2% of the total mass of the liquid fertilizer. Ultrasonic dispersion treatment is then performed. The ultrasonic frequency is 30kHz-40kHz, the power is 800W-1000W, and the treatment time is 20min-30min. Finally, the pH value of the fertilizer is adjusted to 6.8-7.2 to obtain a stable liquid biological fertilizer product.

[0011] As a further solution of the present invention: in the step 5, a corrosion-resistant collection tank is used to collect the waste liquid generated in steps 1 to 4, a mixture of calcium hydroxide and sodium carbonate is added thereto, and the mixture is stirred and mixed until the pH value of the waste liquid reaches 7-8, and the separated waste liquid is filtered and separated after precipitation to obtain a separated waste liquid, and the separated waste liquid is used to produce biological fertilizer according to the steps of lincomycin waste residue.

[0012] As a further solution of the present invention: in the step five, the quality inspection of the finished liquid biological fertilizer includes physical and chemical index inspection and safety inspection. After the inspection is qualified, the finished liquid biological fertilizer is filled in food-grade plastic barrels, and after completion, it is placed in a warehouse for light-proof and ventilated storage.

[0013] By adopting the above technical solution, compared with the prior art, the beneficial effects of the present invention are: 1. The present invention uses dilute hydrochloric acid and sodium hydroxide solution to alternately soak the lincomycin waste residue, which can fully dissolve various impurities on the surface and inside of the residue. Under acidic conditions, it can decompose alkaline harmful substances and pollutants combined with metal ions. Under alkaline conditions, it can decompose acidic residual antibiotics and organic substances. In the continuous aeration process, tiny bubbles can wash the surface of the residue, making it easier for impurities to fall off and dissolve, and on the other hand, increase the dissolved oxygen content in the liquid, creating favorable conditions for the subsequent survival of microorganisms, and also contribute to the oxidation and decomposition of harmful substances, so that the residue enters the The residue is initially purified before the subsequent treatment steps. By using high-pressure cooking, the synergistic effect of high temperature, high pressure and ethanol can more thoroughly destroy the chemical structure of antibiotics such as lincomycin, making them inactive and decomposing them into small molecules, which is beneficial to the preparation of liquid fertilizer in the subsequent steps. The cooking liquid mixed with deionized water and ethanol can penetrate into the internal pores of the residue and bring out more harmful substances hidden inside. The intermittent stirring during the cooking process can ensure that the residue is heated evenly, avoid local overheating or insufficient cooking, make the treatment effect more uniform, and produce high-quality, stable and environmentally friendly biological fertilizer. 2. The present invention adds a composite enzyme preparation, wherein the enzymatic hydrolysis can specifically decompose the macromolecular organic matter in the fungus residue, and convert it into small molecular nutrients that can be directly used by microorganisms, thereby improving the availability of nutrients. The activated carbon can further remove small molecular harmful substances and residual antibiotic decomposition products produced during the enzymatic hydrolysis process, so that the fungus residue is deeply purified, providing a better matrix for microbial inoculation. By selecting a composite bacterial agent of Bacillus subtilis, Bacillus jelly and Rhodopseudomonas palustris, Bacillus subtilis has a strong ability to decompose organic matter and produce antibacterial substances, which is helpful to inhibit the growth of miscellaneous bacteria and decompose complex organic matter, and Bacillus jelly can also be used to produce antibacterial substances. Bacillus can convert difficult-to-use elements such as phosphorus and potassium in the soil into forms that can be absorbed by plants, thereby increasing the nutrient content of fertilizers. Rhodopseudomonas palustris can carry out photosynthesis under light conditions to produce oxygen and nutrients, providing a favorable living environment for microorganisms. It can also enhance the nitrogen fixation effect of bacterial fertilizers. By adding calcium hydroxide and sodium carbonate to adjust the pH value of the waste liquid and performing precipitation and filtration, pollutants such as heavy metal ions and residual organic matter in the waste liquid are removed. The recovered filtrate is used again for residue treatment, realizing the recycling of water resources, reducing the pressure on the environment from lincomycin waste residue and waste liquid discharge, and realizing efficient resource utilization of lincomycin waste residue. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a schematic diagram of the process of preparing fertilizer from lincomycin waste residue in an embodiment of the present invention. DETAILED DESCRIPTION

[0015] The specific embodiments of the present invention are further described below in conjunction with the accompanying drawings. It should be noted that the description of these embodiments is used to help understand the present invention, but does not constitute a limitation of the present invention. In addition, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0016] The invention discloses a method for preparing biological fertilizer from lincomycin waste residue, comprising pretreatment of lincomycin waste residue, microbial inoculation, bacterial fermentation, trace element addition and liquid fertilizer finished product detection. The specific operation steps of producing biological fertilizer from lincomycin waste residue are as follows: Step 1: Place the lincomycin waste residue in a cleaning tank and add acid and alkali solutions to alternately soak it for pretreatment, and after the treatment, rinse it with clean water until it is neutral, and continue aeration during the treatment process to obtain the cleaned residue; Step 2: Add the cleaned mushroom residue into a high-pressure cooking pot, add cooking liquid and cook at high temperature, add a composite enzyme preparation for enzymolysis after cooling, and add activated carbon for adsorption to obtain purified mushroom residue; Step 3, selecting Bacillus subtilis, Bacillus gelatinus and Rhodopseudomonas palustris as composite microbial agents and activating and culturing them, mixing them after the cultivation and inoculating them into the purified bacterial residue, adding nutrient solution for inoculation and cultivation, and obtaining liquid bacterial fertilizer after the cultivation; Step 4: adding nutrients and trace element solution to the liquid fertilizer, stirring and mixing, adding a polymer stabilizer, and ultrasonically dispersing the mixture to obtain a stable liquid biological fertilizer product; Step 5: Collect and treat the waste liquid generated in step 1 to step 4, and repeat the above steps for secondary fermentation. Perform quality inspection on the obtained liquid bio-fertilizer product, and fill and bag it after passing the inspection.

[0017] In one embodiment of the present invention, in step 1, the lincomycin waste residue is placed in a special corrosion-resistant cleaning tank, and is soaked in a dilute hydrochloric acid solution with a pH value of 3-4 for 30 min-60 min. During the process, stirring is continued at a stirring rate of 30 r / min-50 r / min. After the soaking is completed, the acid is discharged, and it is rinsed to neutrality with clean water, and then soaked in a sodium hydroxide solution with a pH value of 9-10 for 50 min-80 min. During the process, stirring is continued at a stirring rate of 30 r / min-50 r / min. After the soaking is completed, it is rinsed again with clean water to neutrality. During the acid-base alternating soaking, an aeration device is used to continuously introduce tiny bubbles into the cleaning solution, and the ventilation volume is 0.8 m3 per cubic meter of cleaning liquid. 3 -1.2m 3 The cleaning time is controlled at 45min-60min, and after completion, the cleaned fungus residue is obtained.

[0018] In one embodiment of the present invention: in step 2, the cleaned mushroom residue is transferred to a high-pressure cooking pot, and a cooking liquid prepared by mixing deionized water and ethanol solution in a ratio of 3:1 is added, the mass ratio of mushroom residue to cooking liquid is 1:2-3, and the mixture is cooked at 110°C-130°C and a pressure of 0.15MPa-0.25MPa for 60min-90min. Intermittent stirring is adopted during the cooking process, and the mixture is stirred for 10min and then stopped for 5min. The stirring speed is 60r / min-80r / min. After the cooking is completed, the standby mushroom residue is obtained.

[0019] In one embodiment of the present invention: in step 2, the spare mushroom residue after cooking is cooled to 40°C-50°C, and then a composite enzyme preparation is added for enzymatic hydrolysis, wherein the composite enzyme preparation is prepared by mixing lipase, mannanase and chitin in a ratio of 3:2:1, and the addition amount of the composite enzyme preparation is 40-60 enzyme activity units per kilogram of the spare mushroom residue. At the same time, activated carbon powder accounting for 2%-3% of the mass of the spare mushroom residue is added, and enzymatic hydrolysis is carried out for 3h-4h at a pH value of 6-7, and purified mushroom residue is obtained after adsorption is completed.

[0020] In one embodiment of the present invention, in step 3, Bacillus subtilis, Bacillus jelly-like and Rhodopseudomonas palustris are selected as composite microbial agents, and these three bacteria are activated and cultured in their respective activation culture media. The specific components of the three groups of activation culture media are as follows: The activation medium for Bacillus subtilis contains 10g / L-12g / L of peptone, 5g / L-8g / L of beef extract and 3g / L-5g / L of sodium chloride, the activation medium for Bacillus gelatinus contains 8g / L-10g / L of sucrose, 2g / L-3g / L of ammonium sulfate and 1g / L-2g / L of dipotassium hydrogen phosphate, and the activation medium for Rhodopseudomonas palustris contains 3g / L-5g / L of sodium acetate, 1g / L-2g / L of sodium bicarbonate and 0.5g / L-1g / L of magnesium sulfate. The three bacteria are inoculated in corresponding culture media respectively and cultured at 30℃-32℃ for 24h-36h respectively. The three activated bacteria are mixed in a volume ratio of 2:2:1 to prepare a composite microbial culture solution, and the composite microbial culture solution is inoculated into the purified bacterial residue in a mass ratio of 1:5-8 to the pretreated bacterial residue.

[0021] In one embodiment of the present invention: in step three, a mixed nutrient solution containing 3g / L-5g / L of glucose, 1g / L-2g / L of yeast powder and 2g / L-3g / L of corn syrup is added to the purified bacterial residue inoculated with the composite microbial bacterial liquid, and cultured for 4d-5d at a temperature of 33°C-35°C and a light intensity of 2000lx-3000lx. During the culture process, slow stirring is performed every 8h at a stirring speed of 30r / min-40r / min. After mixing, liquid bacterial fertilizer is obtained.

[0022] In one embodiment of the present invention: in step 4, after the liquid bacterial fertilizer is cultured, a fine powder is added thereto, which is ground by mixing fish bone meal, soybean meal meal and seaweed meal in a ratio of 2:3:1, and the particle size of the fine powder is between 0.08 mm and 0.1 mm, and the addition amount is 5%-8% of the total mass of the liquid bacterial fertilizer. At the same time, a trace element solution is added, which contains boron, zinc and molybdenum elements, and the concentrations are 10 mg-15 mg of boron, 20 mg-30 mg of zinc and 5 mg-8 mg of molybdenum per liter of solution. The amount of chitosan and xanthan gum is 0.5%-1% of the volume of the bacterial liquid. After mixing, a natural polymer stabilizer is added. A mixture of chitosan and xanthan gum is selected. Chitosan and xanthan gum are mixed in a mass ratio of 3:2. The amount of addition is 1%-2% of the total mass of the liquid fertilizer. Ultrasonic dispersion treatment is then performed. The ultrasonic frequency is 30kHz-40kHz, the power is 800W-1000W, and the treatment time is 20min-30min. Finally, the pH value of the fertilizer is adjusted to 6.8-7.2 to obtain a stable liquid biological fertilizer product.

[0023] In one embodiment of the present invention: in step five, a corrosion-resistant collection tank is used to collect the waste liquid generated in steps one to four, a mixture of calcium hydroxide and sodium carbonate is added thereto, and the mixture is stirred and mixed until the pH value of the waste liquid reaches 7-8, and the separated waste liquid is filtered and separated after precipitation to obtain a separated waste liquid, and the separated waste liquid is used to produce biological fertilizer according to the steps of lincomycin waste residue.

[0024] In one embodiment of the present invention: In step five, the quality inspection of the finished liquid biological fertilizer includes physical and chemical index inspection and safety inspection. After passing the inspection, the finished liquid biological fertilizer is filled in food-grade plastic barrels, and after completion, it is placed in a warehouse for light-proof and ventilated storage.

[0025] In one embodiment of the present invention: when the separated waste liquid is subjected to secondary treatment, the treated filtrate and the newly collected lincomycin waste residue are accurately weighed, and the two are mixed in a fermentation stirring kettle at a mass ratio of 1:3-4. The stirring kettle has heating and humidity adjustment functions, and the water content of the mixture is adjusted to 50%-60% by spraying deionized water into the kettle or turning on a heating evaporation device. At the same time, nutrients are added to the stirring kettle, and urea is agricultural grade granular urea, which is added at 1%-2% of the mass of the mixture. Potassium dihydrogen phosphate is selected from industrial grade crystals, and the addition amount is 0.5%-1% of the mass of the mixture. The bran is high-quality wheat bran, and the particle size after pulverization is less than 1mm, and the addition amount is 3%-5% of the mass of the mixture. The same composite microbial strains as those for the first fermentation are taken out from the strain storage. The activated culture medium was sterilized in an autoclave at 121°C and 0.15MPa for 30 minutes in advance. The activated bacterial agent was increased to 10%-12% of the mass of the mixed material according to the inoculation amount, and was evenly sprayed on the mixed material using a spray inoculation device. The stirring kettle was stirred at a speed of 60r / min-80r / min for 15min-20min to ensure that the bacterial agent and the material were fully mixed. The inoculated material was then transported to the fermentation tank by a screw conveyor. The temperature was controlled at 35℃-37℃ in the early stage (0d-2d), raised to 38℃-40℃ in the middle stage (2d-4d), and dropped back to 35℃-37℃ in the late stage (4d-6d). The ventilation system used a centrifugal fan, and the air was filtered through a high-efficiency air filter and evenly introduced through the bottom ventilation duct.

[0026] In one embodiment of the present invention: the material after the secondary fermentation is transported to a drying device through a belt conveyor for drying treatment. The drying device uses a low-temperature hot air circulation dryer. The hot air is provided by a gas hot air furnace. The hot air temperature is accurately controlled at 50°C-60°C by a temperature controller. The residence time of the material in the dryer is controlled by adjusting the conveyor belt speed to ensure that the material is dried until the moisture content drops to 20%-25%. The thermal efficiency of the drying device can reach 70%-80% through the optimization of the heat recovery device and the insulation layer. The dried material is transported to a pulverizer for pulverization. A hammer pulverizer is selected. The hammer head of the pulverizer is made of high chromium alloy. The material is pulverized to a particle size controlled at 0.5mm-1mm by adjusting the sieve aperture. The pulverized material is transported to a storage bin through a screw elevator to wait for subsequent processing or to be used as a raw material for the preparation of liquid fertilizer again.

[0027] In one embodiment of the present invention: the physical and chemical indicators include pH value detection, nutrient content determination and microbial quantity detection, and the safety detection includes antibiotic residue detection and heavy metal detection.

[0028] Embodiment 1 Take 100 kg of lincomycin waste residue, place it in a special corrosion-resistant cleaning tank, add dilute hydrochloric acid solution with a pH value of 3.5 and soak for 45 minutes, stirring at a rate of 40 r / min, drain the acid after soaking, rinse with clean water until neutral, then add sodium hydroxide solution with a pH value of 9.5 and soak for 60 minutes, stirring at a rate of 40 r / min. During the acid-base alternating soaking process, use an aeration device to continuously introduce tiny bubbles into the cleaning solution, and the ventilation volume is 1m3 per cubic meter of cleaning liquid. 3 Gas, cleaning time is 50min; The cleaned mushroom residue is transferred to a high-pressure cooking pot, and a cooking liquid mixed with deionized water and ethanol solution in a ratio of 3:1 is added, and the mass ratio of mushroom residue to cooking liquid is 1:2.5. It is cooked at 120°C and a pressure of 0.2MPa for 75 minutes. Intermittent stirring is adopted during the cooking process. After stirring for 10 minutes, the stirring speed is 70r / min. After the cooking is completed, the spare mushroom residue is cooled to 45°C, and a composite enzyme preparation is added for enzymatic hydrolysis. The composite enzyme preparation is prepared by mixing lipase, mannanase and chitin in a ratio of 3:2:1, and the addition amount is 50 enzyme activity units per kilogram of spare mushroom residue. At the same time, activated carbon powder accounting for 2.5% of the mass of the spare mushroom residue is added, and the enzymatic hydrolysis is carried out for 3.5 hours at a pH value of 6.5; Bacillus subtilis, Bacillus jelly and Rhodopseudomonas palustris were selected as composite microbial agents, and the three bacteria were activated and cultured in their respective activation culture media. The activation culture medium for Bacillus subtilis contained 11 g / L peptone, 6 g / L beef extract and 4 g / L sodium chloride, the activation culture medium for Bacillus jelly contained 9 g / L sucrose, 2.5 g / L ammonium sulfate and 1.5 g / L potassium dihydrogen phosphate, and the activation culture medium for Rhodopseudomonas palustris contained 4 g / L sodium acetate, 1.5 g / L sodium bicarbonate and 0.7 g / L magnesium sulfate. The three bacteria were inoculated in the corresponding culture medium. The mixture was inoculated into the purified slag at a mass ratio of 1:6, and a mixed nutrient solution containing 4 g / L glucose, 1.5 g / L yeast powder and 2.5 g / L corn steep liquor was added to the purified slag inoculated with the composite microbial solution. The mixture was cultured at a temperature of 34 °C and a light intensity of 2500 lx for 4.5 days. During the culture process, the mixture was slowly stirred every 8 hours at a speed of 35 r / min. After the liquid fertilizer is cultivated, fine powder ground from fish bone meal, soybean meal and seaweed meal in a ratio of 2:3:1 is added thereto, the particle size of the fine powder is 0.09 mm, and the addition amount is 6.5% of the total mass of the liquid fertilizer. The trace element solution contains boron, zinc and molybdenum, and the concentrations are 12 mg of boron, 25 mg of zinc and 6 mg of molybdenum per liter of solution, and the addition amount is 0.7% of the volume of the bacterial solution. A natural polymer stabilizer is added, chitosan and xanthan gum are mixed in a mass ratio of 3:2, and the addition amount is 1.5% of the total mass of the liquid fertilizer. Then, it is dispersed by ultrasound, the ultrasonic frequency is 35 kHz, the power is 900 W, and the processing time is 25 min. Finally, the pH value of the fertilizer is adjusted to 7 to obtain a stable liquid biological fertilizer product.

[0029] Embodiment 2 Take 80kg of lincomycin waste residue, place it in a special corrosion-resistant cleaning tank, add dilute hydrochloric acid solution with a pH value of 3.2 and soak for 50 minutes, stirring at a rate of 35r / min, drain the acid after soaking, rinse with clean water until neutral, then add sodium hydroxide solution with a pH value of 9.2 and soak for 70 minutes, stirring at a rate of 35r / min. During the acid-base alternating soaking process, use an aeration device to continuously introduce tiny bubbles into the cleaning solution, and the ventilation volume is 0.9m3 per cubic meter of cleaning liquid. 3 , the cleaning time is 55min; The cleaned mushroom residue was transferred to a high-pressure cooking pot, and a cooking liquid mixed with deionized water and ethanol solution in a ratio of 3:1 was added, and the mass ratio of mushroom residue to cooking liquid was 1:2.2. The mixture was cooked at 115°C and a pressure of 0.18MPa for 80 minutes. Intermittent stirring was adopted during the cooking process. The mixture was stirred for 10 minutes and then stopped for 5 minutes. The stirring speed was 65r / min. After the cooking was completed, the standby mushroom residue was cooled to 42°C, and a composite enzyme preparation was added for enzymatic hydrolysis. The composite enzyme preparation was prepared by mixing lipase, mannanase and chitin in a ratio of 3:2:1. The addition amount was 45 enzyme activity units per kilogram of standby mushroom residue. At the same time, activated carbon powder accounting for 2.2% of the mass of the standby mushroom residue was added, and the mixture was enzymatically hydrolyzed for 3.2 hours at a pH value of 6.2. Bacillus subtilis, Bacillus jelly and Rhodopseudomonas palustris were selected as composite microbial agents, and the three bacteria were activated and cultured in their respective activation medium. The activation medium for Bacillus subtilis contained 10.5 g / L of peptone, 5.5 g / L of beef extract and 3.5 g / L of sodium chloride, the activation medium for Bacillus jelly contained 8.5 g / L of sucrose, 2.2 g / L of ammonium sulfate and 1.2 g / L of potassium dihydrogen phosphate, and the activation medium for Rhodopseudomonas palustris contained 3.5 g / L of sodium acetate, 1.2 g / L of sodium bicarbonate and 0.6 g / L of magnesium sulfate. The three bacteria were inoculated in the corresponding The culture medium was incubated at 30.5°C for 32 hours respectively, the three activated bacteria were mixed in a volume ratio of 2:2:1 to prepare a composite microbial liquid, and the composite microbial liquid was inoculated into the purified residue in a mass ratio of 1:7 to the pretreated residue, and a mixed nutrient solution containing 3.5 g / L glucose, 1.2 g / L yeast powder and 2.2 g / L corn steep liquor was added to the purified residue inoculated with the composite microbial liquid, and the mixture was cultured at a temperature of 33.5°C and a light intensity of 2200 lx for 4.8 days. During the culture process, the mixture was slowly stirred every 8 hours at a stirring speed of 32 r / min. After the liquid fertilizer is cultivated, a fine powder is added thereto which is ground from fish bone meal, soybean meal meal and seaweed meal in a ratio of 2:3:1. The particle size of the fine powder is 0.085 mm, and the amount added is 7% of the total mass of the liquid fertilizer. A trace element solution is added, which contains boron, zinc and molybdenum elements. The concentrations are 11 mg of boron, 22 mg of zinc and 5.5 mg of molybdenum per liter of solution, and the amount added is 0.6% of the volume of the bacterial solution. A natural polymer stabilizer is added, and a mixture of chitosan and xanthan gum is selected. Chitosan and xanthan gum are mixed in a mass ratio of 3:2, and the amount added is 1.2% of the total mass of the liquid fertilizer. Then, it is dispersed by ultrasound, with an ultrasonic frequency of 32 kHz, a power of 850 W, and a processing time of 22 min. Finally, the pH value of the fertilizer is adjusted to 6.9 to obtain a stable liquid biological fertilizer product.

[0030] Embodiment 3 Take 120kg of lincomycin waste residue, place it in a special corrosion-resistant cleaning tank, add dilute hydrochloric acid solution with a pH value of 3.8 and soak for 35 minutes, stirring at a rate of 45r / min. After soaking, drain the acid and rinse with clean water until neutral. Then add sodium hydroxide solution with a pH value of 9.8 and soak for 55 minutes, stirring at a rate of 45r / min. During the acid-base alternating soaking process, use an aeration device to continuously introduce tiny bubbles into the cleaning solution, and the ventilation volume is 1.1m per cubic meter of cleaning liquid. 3 , the cleaning time is 48min; The cleaned mushroom residue was transferred to a high-pressure cooking pot, and a cooking liquid mixed with deionized water and ethanol solution in a ratio of 3:1 was added, and the mass ratio of mushroom residue to cooking liquid was 1:2.8. The mixture was cooked at 125°C and a pressure of 0.22MPa for 65 minutes. Intermittent stirring was adopted during the cooking process. The mixture was stirred for 10 minutes and then stopped for 5 minutes. The stirring speed was 75r / min. After the cooking was completed, the standby mushroom residue was cooled to 48°C, and a composite enzyme preparation was added for enzymatic hydrolysis. The composite enzyme preparation was prepared by mixing lipase, mannanase and chitin in a ratio of 3:2:1, and the addition amount was 55 enzyme activity units per kilogram of standby mushroom residue. At the same time, activated carbon powder accounting for 2.8% of the mass of the standby mushroom residue was added, and the mixture was enzymatically hydrolyzed for 3.8 hours at a pH value of 6.8. Bacillus subtilis, Bacillus jelly and Rhodopseudomonas palustris were selected as composite microbial agents, and the three bacteria were activated and cultured in their respective activation medium. The activation medium for Bacillus subtilis contained 11.5 g / L of peptone, 7.5 g / L of beef extract and 4.5 g / L of sodium chloride, the activation medium for Bacillus jelly contained 9.5 g / L of sucrose, 2.8 g / L of ammonium sulfate and 1.8 g / L of potassium dihydrogen phosphate, and the activation medium for Rhodopseudomonas palustris contained 4.5 g / L of sodium acetate, 1.8 g / L of sodium bicarbonate and 0.8 g / L of magnesium sulfate. The three bacteria were inoculated in the corresponding culture medium. The culture medium was prepared by mixing the three activated bacteria in a volume ratio of 2:2:1 to obtain a composite microbial liquid, and the composite microbial liquid was inoculated into the purified residue in a mass ratio of 1:5.5 to the pretreated residue. A mixed nutrient solution containing 4.5 g / L glucose, 1.8 g / L yeast powder and 2.8 g / L corn steep liquor was added to the purified residue inoculated with the composite microbial liquid, and the mixture was cultured at a temperature of 34.5°C and a light intensity of 2800 lx for 4.2 days. During the culture process, the mixture was slowly stirred every 8 hours at a stirring speed of 38 r / min. After the liquid fertilizer is cultivated, a fine powder is added thereto, which is ground from fish bone meal, soybean meal meal and seaweed meal in a ratio of 2:3:1. The particle size of the fine powder is 0.095 mm, and the addition amount is 5.5% of the total mass of the liquid fertilizer. A trace element solution is added, which contains boron, zinc and molybdenum elements. The concentrations are 13 mg of boron, 28 mg of zinc and 7 mg of molybdenum per liter of solution, and the addition amount is 0.8% of the volume of the bacterial solution. A natural polymer stabilizer is added, and a mixture of chitosan and xanthan gum is selected. Chitosan and xanthan gum are mixed in a mass ratio of 3:2, and the addition amount is 1.8% of the total mass of the liquid fertilizer. Then, it is dispersed by ultrasound, with an ultrasonic frequency of 38 kHz, a power of 950 W, and a processing time of 28 min. Finally, the pH value of the fertilizer is adjusted to 7.1 to obtain a stable liquid biological fertilizer product.

[0031] Embodiment 4: Take 90kg of lincomycin waste residue, place it in a special corrosion-resistant cleaning tank, add dilute hydrochloric acid solution with a pH value of 3.4 and soak for 40 minutes, stirring at a rate of 38r / min. After soaking, drain the acid and rinse with clean water until neutral. Then add sodium hydroxide solution with a pH value of 9.4 and soak for 65 minutes, stirring at a rate of 38r / min. During the acid-base alternating soaking process, use an aeration device to continuously introduce tiny bubbles into the cleaning solution, and the ventilation volume is 0.95m3 per cubic meter of cleaning liquid. 3 , the cleaning time is 52min; The cleaned mushroom residue was transferred to a high-pressure cooking pot, and a cooking liquid mixed with deionized water and ethanol solution in a ratio of 3:1 was added, and the mass ratio of mushroom residue to cooking liquid was 1:2.4. The mixture was cooked at 118°C and a pressure of 0.21MPa for 70 minutes. Intermittent stirring was adopted during the cooking process, and the mixture was stopped for 5 minutes after every 10 minutes of stirring. After the cooking was completed, the standby mushroom residue was cooled to 44°C, and a composite enzyme preparation was added for enzymatic hydrolysis. The composite enzyme preparation was prepared by mixing lipase, mannanase and chitin in a ratio of 3:2:1, and the addition amount was 48 enzyme activity units per kilogram of standby mushroom residue. At the same time, activated carbon powder accounting for 2.4% of the mass of the standby mushroom residue was added, and the enzymatic hydrolysis was carried out for 3.4 hours at a pH value of 6.4. Bacillus subtilis, Bacillus jelly and Rhodopseudomonas palustris were selected as composite microbial agents, and the three bacteria were activated and cultured in their respective activation culture media. The activation culture media for Bacillus subtilis contained 10.8 g / L of peptone, 6.5 g / L of beef extract and 3.8 g / L of sodium chloride, the activation culture media for Bacillus jelly contained 9.2 g / L of sucrose, 2.6 g / L of ammonium sulfate and 1.6 g / L of potassium dihydrogen phosphate, and the activation culture media for Rhodopseudomonas palustris contained 4.2 g / L of sodium acetate, 1.6 g / L of sodium bicarbonate and 0.7 g / L of magnesium sulfate. The three bacteria were inoculated in the corresponding culture media. The culture medium was added and cultured at 31.2℃ for 31h respectively. The three activated bacteria were mixed in a volume ratio of 2:2:1 to prepare a composite microbial liquid. The composite microbial liquid was inoculated into the purified residue in a mass ratio of 1:6.5 to the pretreated residue. A mixed nutrient solution containing 4.2g / L glucose, 1.6g / L yeast powder and 2.6g / L corn steep liquor was added to the purified residue inoculated with the composite microbial liquid. The mixture was cultured at a temperature of 34.2℃ and a light intensity of 2600lx for 4.6d. During the culture process, the mixture was slowly stirred every 8h at a stirring speed of 36r / min. After the liquid fertilizer is cultivated, a fine powder is added thereto, which is ground from fish bone meal, soybean meal meal and seaweed meal in a ratio of 2:3:1. The particle size of the fine powder is 0.092 mm, and the addition amount is 6% of the total mass of the liquid fertilizer. A trace element solution is added, which contains boron, zinc and molybdenum elements. The concentrations are 12.5 mg of boron, 26 mg of zinc and 6.5 mg of molybdenum per liter of solution, and the addition amount is 0.75% of the volume of the bacterial solution. A natural polymer stabilizer is added, and a mixture of chitosan and xanthan gum is selected. Chitosan and xanthan gum are mixed in a mass ratio of 3:2, and the addition amount is 1.4% of the total mass of the liquid fertilizer. Then, it is dispersed by ultrasound, with an ultrasonic frequency of 36 kHz, a power of 920 W, and a processing time of 26 min. Finally, the pH value of the fertilizer is adjusted to 7.05 to obtain a stable liquid biological fertilizer product.

[0032] The quality of the liquid biofertilizer products prepared in the above four groups of embodiments was tested, and the testing method was as follows: Nutritional testing, The nitrogen content was determined by the Kjeldahl method using a fully automatic Kjeldahl nitrogen analyzer. The sample digestion time was 1h-2h and the distillation time was 5min-10min. The phosphorus content was determined by the molybdenum antimony colorimetric method using a visible spectrophotometer. The absorbance was measured at a wavelength of 700nm and the phosphorus content was calculated based on the standard curve. The potassium content was determined by flame photometry with a detection accuracy of ±0.5mg / L.

[0033] Microbiological index detection, The plate count method was adopted to culture and count Bacillus subtilis, Bacillus gelatinus and Rhodopseudomonas palustris using nutrient agar medium, beef extract peptone medium, etc., respectively. The incubator temperature was controlled at 30℃-32℃, the incubation time was 48h-72h, and the effective live bacteria count was detected.

[0034] pH value detection, A high-precision laboratory pH meter with a measurement accuracy of ±0.01 was used to test multiple samples, with each sample measured 3-5 times and the average value taken to ensure that the pH value was within the range of 6.8-7.2.

[0035] Safety testing, Detection of antibiotic residues: Through high performance liquid chromatography-mass spectrometry, the chromatographic column is selected as C18 column, the column temperature is 30-40℃, the mobile phase is methanol-water system, the flow rate is 0.2-0.5mL / min, the injection volume is 5-10uL, the mass spectrometry conditions are electrospray ion source, positive ion or negative ion scanning mode, the lincomycin residue should be lower than Bmg / L.

[0036] Heavy metal detection: Atomic absorption spectrometry is used for heavy metal detection such as lead, cadmium, and mercury. The wavelength of the lead hollow cathode lamp is 283.3nm, the wavelength of the cadmium hollow cathode lamp is 228.8nm, and the wavelength of the mercury hollow cathode lamp is 253.7nm. The flame type is selected according to the characteristics of the elements. Microwave digestion is used for sample pretreatment. The digestion tank is made of polytetrafluoroethylene, the digestion temperature is 180℃-220℃, and the digestion time is 20min-30min.

[0037] The calculation results are as follows: Embodiment 1

[0038] Nutritional content: nitrogen content is 3.5%, phosphorus content is 2.2%, potassium content is 1.8%; Microbiological indicators: The effective viable count of the three strains is 5.0×10 8 CFU / g; pH: 7.0; Antibiotic residue detection: 0.05mg / L; Heavy metal detection: None.

[0039] Embodiment 2 Nutritional content: nitrogen content is 3.2%, phosphorus content is 2.0%, potassium content is 1.6%; Microbiological indicators: The effective viable count of the three strains is 4.5×10 8 CFU / g; pH: 6.9; Antibiotic residue detection: 0.06mg / L; Heavy metal detection: None.

[0040] Embodiment 3 Nutritional content: nitrogen content is 3.8%, phosphorus content is 2.4%, potassium content is 2.0%; Microbiological indicators: The effective viable count of the three strains was 5.5×10 8 CFU / g; pH: 7.2; Antibiotic residue detection: 0.04mg / L; Heavy metal detection: None.

[0041] Embodiment 4: Nutritional content: nitrogen content is 3.4%, phosphorus content is 2.1%, potassium content is 1.7%; Microbiological indicators: The effective viable count of the three strains was 4.8×10 8 CFU / g; pH: 7.1; Antibiotic residue detection: 0.05mg / L; Heavy metal detection: None.

[0042] In summary, the feasibility and effectiveness of preparing biological fertilizer from lincomycin waste residue in the four groups of embodiments of the present invention have important practical significance and application value.

[0043] The embodiments of the present invention are described in detail above with reference to the accompanying drawings, but the present invention is not limited to the described embodiments. For those skilled in the art, various changes, modifications, substitutions and variations of these embodiments are made without departing from the principles and spirit of the present invention, and still fall within the scope of protection of the present invention.

[0044] In the description of the specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0045] The above contents are merely examples and explanations of the present invention. Various modifications or additions to the specific embodiments described or replacements in similar ways by technicians in the technical field shall fall within the protection scope of the present invention as long as they do not deviate from the invention or exceed the scope defined by the claims.

[0046] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A method for preparing biological fertilizer from lincomycin waste residue, comprising pretreatment of lincomycin waste residue, microbial inoculation, bacterial fermentation, trace element addition and liquid fertilizer finished product testing, characterized in that: The specific operation steps of producing biological fertilizer from lincomycin waste residue are as follows: Step 1: placing the lincomycin waste residue in a cleaning tank and adding acid and alkali solutions for alternate soaking pretreatment, with continuous aeration during the treatment process to obtain the cleaned residue; Step 2: Add the cleaned mushroom residue into a high-pressure cooking pot, add cooking liquid and cook at high temperature, add a composite enzyme preparation for enzymolysis after cooling, and add activated carbon for adsorption to obtain purified mushroom residue; Step 3: Select a composite microbial agent and activate and culture it. After the culture is completed, mix it and inoculate it into the purified bacterial residue, add nutrient solution for inoculation and culture, and obtain a liquid bacterial fertilizer after the culture is completed; Step 4: adding nutrients and trace element solution to the liquid fertilizer, stirring and mixing, adding a polymer stabilizer, and ultrasonically dispersing the mixture to obtain a stable liquid biological fertilizer product; Step 5: Collect and treat the waste liquid generated in step 1 to step 4, and repeat the above steps for secondary fermentation. Perform quality inspection on the obtained liquid bio-fertilizer product, and fill and bag it after passing the inspection.

2. The method for preparing biological fertilizer from lincomycin waste residue according to claim 1, characterized in that: In the step 1, the lincomycin waste residue is placed in a special corrosion-resistant cleaning tank, and is soaked in a dilute hydrochloric acid solution with a pH value of 3-4 for 30 minutes to 60 minutes. During the process, stirring is continued at a stirring rate of 30 r / min-50 r / min. After the soaking is completed, the acid is discharged, and it is rinsed to neutrality with clean water, and then soaked in a sodium hydroxide solution with a pH value of 9-10 for 50 minutes to 80 minutes. During the process, stirring is continued at a stirring rate of 30 r / min-50 r / min. After the soaking is completed, it is rinsed again with clean water to neutrality. During the acid-base alternating soaking, an aeration device is used to continuously introduce tiny bubbles into the cleaning solution, and the ventilation volume is 0.8m per cubic meter of cleaning liquid. 3 -1.2m 3 The cleaning time is controlled at 45min-60min, and after completion, the cleaned fungus residue is obtained.

3. The method for preparing biological fertilizer from lincomycin waste residue according to claim 1, characterized in that: In the step 2, the cleaned mushroom residue is transferred to a high-pressure cooking pot, and a cooking liquid prepared by mixing deionized water and ethanol solution in a ratio of 3:1 is added, the mass ratio of the mushroom residue to the cooking liquid is 1:2-3, and the mixture is cooked at 110°C-130°C and a pressure of 0.15MPa-0.25MPa for 60min-90min. Intermittent stirring is adopted during the cooking process, and the mixture is stirred for 10min and then stopped for 5min. The stirring speed is 60r / min-80r / min. After the cooking is completed, the standby mushroom residue is obtained.

4. The method for preparing biological fertilizer from lincomycin waste residue according to claim 1, characterized in that: In the step 2, the spare mushroom residue after cooking is cooled to 40°C-50°C, and then a composite enzyme preparation is added for enzymatic hydrolysis, wherein the composite enzyme preparation is prepared by mixing lipase, mannanase and chitin in a ratio of 3:2:1, and the addition amount of the composite enzyme preparation is 40-60 enzyme activity units per kilogram of the spare mushroom residue. At the same time, activated carbon powder accounting for 2%-3% of the mass of the spare mushroom residue is added, and enzymatic hydrolysis is carried out for 3h-4h at a pH value of 6-7. After adsorption is completed, purified mushroom residue is obtained.

5. The method for preparing biological fertilizer from lincomycin waste residue according to claim 1, characterized in that: In the step 3, Bacillus subtilis, Bacillus gelatinus and Rhodopseudomonas palustris are selected as composite microbial agents, and these three bacteria are activated and cultured in their respective activation culture media. The specific components of the three groups of activation culture media are as follows: The activation medium for Bacillus subtilis contains 10g / L-12g / L of peptone, 5g / L-8g / L of beef extract and 3g / L-5g / L of sodium chloride, the activation medium for Bacillus gelatinus contains 8g / L-10g / L of sucrose, 2g / L-3g / L of ammonium sulfate and 1g / L-2g / L of dipotassium hydrogen phosphate, and the activation medium for Rhodopseudomonas palustris contains 3g / L-5g / L of sodium acetate, 1g / L-2g / L of sodium bicarbonate and 0.5g / L-1g / L of magnesium sulfate. The three bacteria are inoculated in corresponding culture media respectively and cultured at 30℃-32℃ for 24h-36h respectively. The three activated bacteria are mixed in a volume ratio of 2:2:1 to prepare a composite microbial culture solution, and the composite microbial culture solution is inoculated into the purified bacterial residue in a mass ratio of 1:5-8 to the pretreated bacterial residue.

6. The method for preparing biological fertilizer from lincomycin waste residue according to claim 1, characterized in that: In the step three, a mixed nutrient solution containing 3g / L-5g / L of glucose, 1g / L-2g / L of yeast powder and 2g / L-3g / L of corn steep liquor is added to the purified bacterial residue inoculated with the composite microbial bacterial liquid, and cultured for 4d-5d at a temperature of 33°C-35°C and a light intensity of 2000lx-3000lx. During the culture process, slow stirring is performed every 8h at a stirring speed of 30r / min-40r / min, and liquid bacterial fertilizer is obtained after mixing.

7. The method for producing biological fertilizer from lincomycin waste residue according to claim 1, characterized in that: In the step 4, after the liquid bacterial fertilizer is cultured, a fine powder obtained by mixing fish bone meal, soybean meal powder and seaweed powder in a ratio of 2:3:1 and grinding the mixture is added thereto, wherein the particle size of the fine powder is between 0.08 mm and 0.1 mm, and the amount added is 5% to 8% of the total mass of the liquid bacterial fertilizer. At the same time, a trace element solution is added, wherein the trace element solution contains boron, zinc and molybdenum, and the concentrations are 10 mg to 15 mg of boron, 20 mg to 30 mg of zinc and 5 mg to 8 mg of molybdenum per liter of solution, and the amount added is 10% to 15% of the volume of the bacterial liquid. 0.5%-1%, add natural polymer stabilizer after mixing them, select a mixture of chitosan and xanthan gum, mix chitosan and xanthan gum in a mass ratio of 3:2, and add 1%-2% of the total mass of the liquid fertilizer, then use ultrasound to disperse it, the ultrasonic frequency is 30kHz-40kHz, the power is 800W-1000W, the processing time is 20min-30min, and finally adjust the pH value of the fertilizer to 6.8-7.2 to obtain a stable liquid biological fertilizer product.

8. The method for producing biological fertilizer from lincomycin waste residue according to claim 1, characterized in that: In the step 5, a corrosion-resistant collection tank is used to collect the waste liquid generated in steps 1 to 4, a mixture of calcium hydroxide and sodium carbonate is added thereto, and the mixture is stirred and mixed until the pH value of the waste liquid reaches 7-8, and the separated waste liquid is filtered and separated after precipitation to obtain a separated waste liquid, and the separated waste liquid is used to produce a bio-fertilizer according to the steps of lincomycin waste residue.

9. The method for producing biological fertilizer from lincomycin waste residue according to claim 1, characterized in that: In the step 5, the quality inspection of the finished liquid bio-fertilizer includes physical and chemical index inspection and safety inspection. After the inspection is qualified, the finished liquid bio-fertilizer is filled in food-grade plastic barrels and placed in a warehouse for light-proof and ventilated storage.

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